comparative studies on biosorption ability of metal oxide nanoparticles using marine micro and macro...
Post on 08-Apr-2016
18 Views
Preview:
DESCRIPTION
TRANSCRIPT
Comparative studies on biosorption ability of metal oxide nanoparticles using marine micro
and macro algae
A. Raj Manoj Reddy & Ankit Kumar TiwariSathyabama University
Biosorption• Biosorption is a physiochemical process that occurs naturally in
certain biomass which allows it to passively concentrate and bind contaminants onto its cellular structure
• Biosorption of metal ions using biological materials such as algae, bacteria, fungal and yeast have received greater attention due to its advantages over conventional method (Arica et al., 2001). It has been defined as the property of biomass such as algae, bacteria, fungal and yeast to bind with metal ions from aqueous solutions (Dursun, 2006; Wang and Chen, 2006; Volesky, 2007).
• The major advantages of biosorption process over conventional technologies include (Kratochvil and Volesky, 1998; Ahalya et al., 2003):
• Low cost • High efficiency • Minimization of sludge production • Biosorbent can be regenerated and • Possible of metals recovery
ObjectiveThis study is being carried out with the following objectives:
• To study the effect of different parameters (pH,time & biomass concentration) on absorption of toxic metal nanoparticles by marine macro alga Kappaphycus alvarezii and micro algae Chlorella vulgaris , Scenedesmus.
• To study the equilibrium and kinetics of heavy metal oxide (ZnO and Fe2O3) removal by freely suspended and immobilized cells of K. alvarezii in batch system.
• To study the desorption /regeneration of metal ions from the immobilized biosorbent.
• To study the best biosorbent based on the percentage of uptake of the metal nanoparticle in three different forms viz., powdered biomass, liquid extract and carrier based seaweed biosorbents.
• To study the characterization of macro and microalgae before and after biosorption process.
MethodologyBiosorbents used• Macro algae- Kappaphycus alvarezii • Micro algae – Scenedesmus sp.
Parameter OptimizationEffect of contact Time, pH and concentration of
metal oxide solutionBiosorption and Desorption of metal ions
Effect of time on absorbents for zinc and iron oxide
Effect of pH on absorbents -for zinc oxide
Effect of pH on absorbents - iron oxideEffect of pH on absorbents - iron oxide
Biosorption and Desorption
250mg of dried biomass
Added to 50ml metal oxide solution
Agitation in a rotary shaker at 180rpm for 3
hrs
Filtration
Metal oxide quantification and further analysis
0.5g of treated biomass
Added to 0.1M HCL
Agitation in rotary shaker at 150rpm for 1 hr
Filtration
Metal quantification
Biosorption Desorption
Characterization studies- Atomic Absorption Spectroscopy
PARAMETER UNIT RESULT
Iron Oxide as Fe2O3 mg/l 12.32Zinc Oxide as ZnO mg/l 7.76
Macro algae
Micro algaePARAMETER UNIT RESULT
Iron Oxide as Fe2O3 mg/l 13.7Zinc Oxide as ZnO mg/l 0.25
Metal uptake capacity of seaweeds
Q= V (Ci - Cf)/SQ - metal ion uptake capacity (mg/g)
Ci= Initial metal concentration in the solution before the sorption analysis (mg/l)
Cf =final concentration of metal in solution after the sorption analysis(mg/l)
S =dry weight of biosorbent (g)V = solution volume (L) (Volesky, 2003).
Metal uptake capacity of seaweeds
S. no
Seaweeds Biomass (g/L) Concentration of metal (mg/g)
1 Kappaphycus alvarezii
2.5 11.072
2 Scenedesmus sp. 2.5 10.52
Iron oxide
Zinc oxideS. no
Seaweeds Biomass (g/L) Concentration of metal (mg/g)
1 Kappaphycus alvarezii
2 16.12
2 Scenedesmus sp. 2.5 15.9
SEM images of macro algae
Iron Oxide Zinc oxide
SEM images of micro algae
Iron Oxide
Zinc Oxide
FT-IR Characterization of Macro algae
Control- Macro algaeSpectrum Name: IR-P-A.sp
4000.0 3000 2000 1500 1000 500 400.00.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3760.73
3399.52
2412.84
2214.10
1572.03
1482.92
1400.18
1263.54
1125.30
923.09
831.85
762.83699.80
651.52618.89
430.39
FT-IR Characterization of Macro algae
Zinc Oxide- Macro algaeSpectrum Name: IR-P-B.sp
4000.0 3000 2000 1500 1000 500 400.00.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3796.31
3402.44
2406.802168.34
1573.42
1397.48
1265.701126.53
922.84
834.59
761.87706.16
651.34
FT-IR Characterization of Macro algae
Iron Oxide- Macro algaeSpectrum Name: IR-P-C.sp
4000.0 3000 2000 1500 1000 500 400.0
0.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3400.99
2171.25
1572.35
1482.11
1400.15
1262.411124.86
923.33
831.75
762.37699.55
651.33
618.39
428.33
FT-IR Characterization of Micro algae
Spectrum Name: IR-P-D.sp
4000.0 3000 2000 1500 1000 500 400.00.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3396.37
2926.97
2855.37
2170.27
1571.72
1481.80
1401.63
1260.79
1121.34
924.53
831.03
762.59697.48
651.46
617.39578.63
428.36
Control- Micro algae
FT-IR Characterization of Micro algae
Zinc Oxide- Micro algaeSpectrum Name: IR-P-E.sp
4000.0 3000 2000 1500 1000 500 400.00.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3395.04
2925.29
2855.12
2169.84
1571.18
1402.60
1258.65
1119.84
829.78
761.64697.60
651.68
616.32
430.00
FT-IR Characterization of Micro algae
Spectrum Name: IR-P-.sp
4000.0 3000 2000 1500 1000 500 400.00.0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100.0
cm-1
%T
3390.71
2922.89
2853.94
2256.37
1570.56
1402.89
1257.51
1118.54
829.16
761.92698.10
652.06615.17
429.89
Iron Oxide- Micro algae
Thank You
top related