details of tune and method setup for ltq orbitrap application: nanospray, peptides gary woffendin,...

Details of Tune and MethodSetup for LTQ Orbitrap

Application: nanospray, peptides

Gary Woffendin, Michaela ScigelovaThermo Electron, UK

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Tune file - nanospray

Micro AS autosampler settings

Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen)Note: in sample table the vial position should be ‘1a1’

Micro AS autosampler settings

Wash solution: water

Micro AS autosampler settings

Surveyor LC settings

Surveyor LC settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

Exclusion duration set to about ½ peak width (here 15 sec)

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settings

LTQ Orbitrap settingsScan event 2 – MS/MS of the 1st most intense ion

LTQ Orbitrap settingsScan event 3 – MS/MS of the 2nd most intense ion

LTQ Orbitrap settingsScan event 4 – MS/MS of the 3rd most intense ion

Divert Valve

Method Summary

Method Summary