details of tune and method setup for ltq orbitrap application: nanospray, peptides gary woffendin,...

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Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

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Page 1: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Details of Tune and MethodSetup for LTQ Orbitrap

Application: nanospray, peptides

Gary Woffendin, Michaela ScigelovaThermo Electron, UK

Page 2: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 3: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 4: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 5: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 6: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 7: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 8: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 9: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Tune file - nanospray

Page 10: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Micro AS autosampler settings

Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen)Note: in sample table the vial position should be ‘1a1’

Page 11: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Micro AS autosampler settings

Wash solution: water

Page 12: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Micro AS autosampler settings

Page 13: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Surveyor LC settings

Page 14: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Surveyor LC settings

Page 15: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 16: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 17: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 18: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Exclusion duration set to about ½ peak width (here 15 sec)

Page 19: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 20: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 21: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 22: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settings

Page 23: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settingsScan event 2 – MS/MS of the 1st most intense ion

Page 24: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settingsScan event 3 – MS/MS of the 2nd most intense ion

Page 25: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

LTQ Orbitrap settingsScan event 4 – MS/MS of the 3rd most intense ion

Page 26: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Divert Valve

Page 27: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Method Summary

Page 28: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

Method Summary