determination of albumin concentration by mip qcm sensor

Determination of Albumin Determination of Albumin Concentration by MIP QCM SensorConcentration by MIP QCM Sensor

Speaker : Speaker : Thong-Yueh Lin Thong-Yueh Lin

Loading curves of different MIP thin filmLoading curves of different MIP thin film

Time (sec)

0 1000 2000 3000 4000 5000 6000 7000

Fre

qu

ence

(H

z)

-250

-200

-150

-100

-50

0

50

DMAPMA-TMPTMADMAPMA-PETTA filmDMAPMA-TEGDMA film

Sample nameDMAPMA-TEGDMA

DMAPMA-TMPTMA

DMAPMA-PETTA

Mass of coating (g) 1.79*10-6 4.119*10-7 1.015*10-5

Adsorption amount (g)

7.10*10-8 8*10-8 5.295*10-8

Adsorption amount (g) per gram MIP

0.04 0.1942 0.005

The amount of adsorption for different The amount of adsorption for different MIP thin filmsMIP thin films

Selectivity of DMAPMA-TMPTMA thin filmSelectivity of DMAPMA-TMPTMA thin film

Cytochrome CLysozym

eAlbumin Myoglobin

Adsorption amount mass ratio of MIP

160 1 1942 30

Adsorption amount mass ratio of non-MIP

13 1 249 86

ConclusionsConclusions

1.1. The protein mixture could be analyzed by HPLC.The protein mixture could be analyzed by HPLC.

22.The MIP thin film coated on Au-OH electrode .The MIP thin film coated on Au-OH electrode

indicated the highest albumin adsorption.indicated the highest albumin adsorption.

3.The MIP thin film coated on Au-NH3.The MIP thin film coated on Au-NH22 electrode electrode

indicated the shortest stable time.indicated the shortest stable time.

= albumin : cytochrome c : myoglobin : lysozyme= albumin : cytochrome c : myoglobin : lysozyme

= 1.4 : 1.0 : 1.5 : 3.1= 1.4 : 1.0 : 1.5 : 3.1

22. Selectivity mole ratio of MIP. Selectivity mole ratio of MIP

= albumin : cytochrome c : myoglobin : lysozyme= albumin : cytochrome c : myoglobin : lysozyme

= 9.1 : 2.9 : 2.8 : 1= 9.1 : 2.9 : 2.8 : 1

ConclusionsConclusions

4.The DMAPMA-TMPTMA MIP thin film 4.The DMAPMA-TMPTMA MIP thin film

showed the highest albumin adsorption showed the highest albumin adsorption

amount.amount.

5.The Selectivity of DMAPMA-TMPTMA thin 5.The Selectivity of DMAPMA-TMPTMA thin

film coated on QCM was cytochrome C: film coated on QCM was cytochrome C:

lysozyme: albumin: myoglobin= lysozyme: albumin: myoglobin=

160:1:1942:30. 160:1:1942:30.

..