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ElA MEDIATED ACTIVATION OF THE ADENOVIRUS E4 PROMOTER.
CHRISTOPHER JONES
A thesis submitted for the degree of
Doctor of Philosophy
at
The University of London,
1993.
Imperial Cancer Research Fund,
Clare Hall Laboratories,
Blanche Lane,
South Mimms,
Herts., EN6 3LD
and
University College London,
Gower Street,
London, WCIE 6BT
ProQuest Number: 10045891
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ABSTRACT
An important function of adenovirus ElA proteins upon an
adenoviral infection of permissive human cells is the transcriptional
activation of viral early genes during the first phase of the lytic cycle. To
this end ElA targets a number of cellular transcription factors, many of
which are sequence specific DNA binding proteins that directly interact
with early viral promoters. Studies of the adenovirus E4 promoter have
identified a promoter element, the ATP binding site, that is critical for
fr^MS-activation by ElA. Several cellular transcription factors interact with
ATE binding sites and are therefore potential targets for ElA.
There is significant evidence that E4F and a member of the ATP
family (ATF-2) can independently function with E lA to activate the
E4 promoter. To assess the role of E4F, I have monitored the effects of
point mutations in the ATF/E4F binding sites on E4 promoter activity
in vivo using a transient expression assay in HeLa cells. I found that
whilst the core motif of these sites is the only upstream requirement for
activation of the E4 promoter, an adjacent point mutation that eliminates
E4F binding (but has no effect on ATE binding) does not affect E4 promoter
activity. These findings indicate that ATE binding sites are the sole
requirement for fraws-activation of the E4 promoter by ElA. I have also
found that some point mutations that strongly reduce E4 promoter
activity in vivo have no effect on binding of ATE-2 to these sites in vitro.
Therefore these results are inconsistent with the suggestion that ATE-2
alone functions with ElA to activate the E4 promoter. Together with the
results of previous studies, these results demonstrate that ElA has
evolved multiple options for activating transcription of early viral genes.
ACKNOWLEDGEMENTS
I would like to thank my supervisors Dr. Kevin Lee (at the Imperial
Cancer Research Fund) and Dr. David Latchman (at University College
London) for their help during my studies. Thanks to Susan Szymkowski
for her valuable comments on the first draft of this thesis and to my
colleagues Norma Masson and Andrew Brown for their help,
encouragement and friendship in the laboratory.
On a non-scientific note, thank you to the following LiDs (Doctors
of Life) for their special contributions: Spencer Howell for saving me from
"the rut"; Mark Hodson, Jonathan Hamill-Keays, Kevin Brotherton,
Christopher Chiverton and David Gaves for thinking that I'm actually
doing something useful; Dave Szymkowski and Christopher J. Jones for
listening to me drone on about ATF-2 and ElA; Philip and Alison
Mitchell for being the most fantastic inspiration (go diamond!); Alan
Tunnacliffe for being incredibly patient whilst I was writing about ElA
instead of thinking about fragile sites; Sharon (I've got a rock on my
finger) Mooney, the only person who would suffer three years of hell
while I further my academic career just because I said I'd marry her when
I'd finished; and of course last, but certainly not least, my parents.
Party on dudes; and be most excellent to each other!
All men dream: But not equally.
Those who dream by night in the dusty
recesses of their minds wake in the day to
find that it was vanity: But the dreamers of
the day are dangerous men, for they may
act their dreams with open eyes, to make it
possible.
T. E. Lawrence, The Seven Pillars of Wisdom.
The winner sees an answer for every
problem; the loser sees a problem for every
answer. The winner sees a green near every
sand trap; the loser sees two or three sand
traps near every green. The winner says "It
may be difficult, but it's possible"; the loser
says "It may be possible, but it's too
difficult". Your choice is, which one do you
want to be?
Don't let anyone steal your dream.
Dexter Yager.
This thesis is dedicated to my parents.
For their love and support, I am eternally grateful.
CONTENTS
ABSTRACT................................................................................................................. 2ACKNOWLEDGEMENTS....................................................................................... 3CONTENTS.................................................................................................................. 6
FIGURES............................................................................................................... 10TABLES................................................................................................................. 12
Chapter 1: Introduction............................................................................................13
1.0 General introduction.......................................................................................... 131.1 The adenovirus replication cycle......................................................................14
1.1.1 The ElA region........................................................................................... 141.1.2 Activation of adenovirus early genes...................................................18
1.2 Mammalian transcription..................................................................................181.3 Control of RNA polymerase II transcription.................................................19
1.3.1 The TATA box........................................................................................... 191.3.2 Upstream promoter elements.................................................................. 20
1.4 Cellular factors involved in transcription by RNApolymerase II........................................................................................................ 211.4.1 RNA polymerase II.....................................................................................211.4.2 General transcription (initiation) factors...............................................22
1.4.2.1 The TATA binding protein........................................................231.4.2.2 Other factors involved in assembly of the
preinitiation complex..................................................................251.4.3 Gene-specific transcription factors.........................................................25
1.5 Trans-activation by adenovirus ElA proteins...............................................271.5.1 A unique conserved region (CR3) of the large ElA protein
is essential for frans-activation by E lA .................................................301.5.2 CR3 contains one copy of a C4 zinc finger motif that is
essential and sufficient for frans-activation.......................................... 321.5.3 CR3 consists of two subdomains............................................................321.5.4 The activating domain of ElA specifically interacts with
TBP............................................................................................................... 351.5.5 ElA can activate transcription in vitro.................................................36
1.6 Characterisation of the adenovirus E4 promoter..........................................371.6.1 Deletional analysis of the E4 promoter................................................ 37
1.6.2 Identification of cellular factor binding sites within theE4 promoter................................................................................................. 39
1.7 Characterisation of proteins that interact with the E4 promoter.................411.7.1 The ATE family......................................................................................... 41
1.7.1.1 Characterisation of ATE binding sites in the viralearly promoters............................................................................ 41
1.7.1.2 Isolation of ATE proteins............................................................ 431.7.1.3 cDNA cloning reveals a multigene family of
transcription factors.................................................................... 431.7.2 E4E................................................................................................................ 471.7.3 Other proteins that bind to the E4 promoter...................................... 48
1.8 Trans-activation of the E4 promoter by E lA ................................................. 481.8.1 E4E mediated frans-activation of the E4 promoter by E lA ..............491.8.2 ATE-2 mediates frans-activation by E lA ............................................. 54
1.9 Aims of this thesis.................................................................................................57
Chapter 2: Analysis of the requirements for E4 promoter activityin vivo.........................................................................................................58
2.0 Methods and Materials....................................................................................... 602.0.1 General strategy of construction of new recombinants.................... 602.0.2 Electrophoretic analysis of nucleic acids.............................................. 62
2.0.2.1 Agarose gel electrophoresis of nucleic acids.......................... 622.0.2.2 Denaturing polyacrylamide gel electrophoresis of
nucleic acids.................................................................................. 622.0.2.3 Gel purification of nucleic acids................................................63
2.0.3 Preparation of plasmid D N A ................................................................. 642.0.3.1 'Maxiprep' DNA............................................................................642.0.3.2 'Miniprep' DNA............................................................................ 65
2.0.4 Preparation of competent bacteria and transformationwith recombinant plasmid D N A .......................................................... 66
2.0.5 Transient transfection assay................................................................... 672.0.5.1 CaCb/phosphate transfection of plasmid DNA into
mammalian cells......................................................................... 672.0.5.2 Assay of chloramphenicol acetyl transferase
activity............................................................................................. 68
2.1 Results......................................................................................................................692.1.0 An ATF binding site that does not bind E4F can mediate
trans-activation of the E4 promoter by E lA ......................................... 692.1.1 Spl binding sites are unable to mediate efficient trans-
activation of the E4 promoter by ElA.....................................................732.1.2 E4F binding is not required for activation of the intact
E4 promoter by E lA ...................................................................................772.1.3 Upstream promoter elements other than the ATF/E4F
binding sites are not required for activation by E lA ..........................832.1.4 E4F binding sites are not required for activation of the
E4 promoter by ElA in Vero cells............................................................84
2.2 Discussion............................................................................................................ 88
Chapter 3: Determination of the DNA binding specificity of anN-terminally truncated ATF-2 protein in vitro................................ 92
3.0 Methods and Materials....................................................................................... 933.0.1 In vitro transcription and translation of ATF proteins......................933.0.2 SDS/polyacrylamide gel electrophoresis of proteins.........................943.0.3 Analysis of the DNA binding activity of proteins using a
gel retardation assay..................................................................................95
3.1 Results.................................................................................................................... 963.1.0 ATF-1 and ATF-2 bind specifically to ATF binding sites
present in various adenovirus early promoters.................................963.1.1 The effect of point mutations on the binding specificity of
ATF-1 and ATF-2.......................................................................................983.1.2 The effect of point mutations on the stability of ATF-2
binding.........................................................................................................1023.1.3 The effect of point mutations on the binding of ATF-2 to
the E4 promoter in vivo............................................................................. 104
3.2 D iscussion............................................................................................................ 109
Chapter 4: Analysis of E4 promoter activity in vitro..........................................114
4.0 Methods and Materials....................................................................................... 1174.0.1 In vitro transcription of the E4 promoter...............................................1174.0.2 Expression and purification of recombinant proteins.......................118
4.1 Results.................................................................................................................... 1204.1.1 Reduction of E4 transcription by the addition of a
competitor oligonucleotide containing an ATF bindingsite ................................................................................................................ 120
4.1.2 Analysis of the effect of preincubation of the nuclear extract with the competitor oligonucleotide on depletionof E4 promoter activity.............................................................................120
4.1.3 The effect of glutathione, thrombin and imidazole uponthe in vitro transcription reaction.........................................................123
4.1.4 Recombinant CREB restores transcription of theE4 promoter by ATF depleted nuclear extracts.................................... 126
4.1.5 A mutant recombinant CREB protein, HôCRlAP, cannot restore transcription of the E4 promoter by ATF depletednuclear extracts...........................................................................................128
4.2 Discussion............................................................................................................ 133
Chapter 5: Discussion................................................................................................. 136
5.1 The relative roles of E4F and ATF-2 in trans-activation of theE4 promoter by ElA.............................................................................................. 1365.1.1 Problems associated with interpreting the results of this
study............................................................................................................. 1385.2 Factors affecting the requirements for trans-activation by E lA ................139
5.2.1 Regulation of other factors by ElA..........................................................1395.2.2 Dimerization of ElA inducible transcription factors......................... 140
5.3 The mechanisms of E4F and ATF-2 mediated trans-activation ofthe E4 promoter by ElA....................................................................................... 141
5.4 Other examples of multiple factors binding to individualpromoter elements................................................................................................ 143
5.5 The role of ATF proteins in other viral trans-activationpathways.................................................................................................................144
5.6 Conclusions......................................................................................................... 146
REFERENCES..............................................................................................................147
Appendix: Abbreviations not described in the text............................................. 168
FIGURES
Figure 1.1: The Ad2 cytoplasmic RNA transcripts, characterised byelectron microscopy of RNAiDNA heteroduplexes................................. 15
Figure 1.1.1: Structures of the transcribed and translated products ofthe Ad2 ElA region..........................................................................................17
Figure 1.4.2.2: Schematic representation of the transcriptionalinitiation of the adenovirus major late promoter.................................... 24
Figure 1.5: General structure of the products of the adenovirus ElA region. A; Structure of the 12S and 13S mRNAs and theirprotein products. B; Structure of CR3 of the large ElA protein.............29
Figure 1.6.2: Summary of results from DNase I footprinting and méthylation interference assay analysis of the adenovirusE4 promoter........................................................................................................40
Figure 1.7.1.3: Amino acid homology in the basic domain andleucine zipper regions of several members of the ATF family and selected other proteins that are known to interact with theE4 promoter........................................................................................................44
Figure 1.8.1: Méthylation interference analysis of E4F and ATFbinding to the -39/-56 site of the E4 promoter............................................. 51
Figure 2.1.0 A: Structure of the E4A38, E4CAT, VIPE4 and E2CATtest promoters......................................................................................................70
Figure 2.1.0 B: Activation of synthetic promoters by ElA;Representative CAT assays...............................................................................71
Figure 2.1.0 C: Activation of synthetic promoters by ElA;Quantitation of CAT assays.............................................................................72
Figure 2.1.1 A: Structure of the SplCAT test promoter...................................... 74Figure 2.1.1 B: Activation of the synthetic promoter SplCAT by
ElA; Representative CAT assays....................................................................75Figure 2.1.1 C: Activation of the synthetic promoter SplCAT by
ElA; Quantitation of CAT assays...................................................................76Figure 2.1.2 A: Structure of the IE4 and WE4 series of promoters.................79
10
Figure 2.1.2 B: Effects of point mutations on E4 promoter activity;Representative CAT assays.............................................................................80
Figure 2.1.2 C: Effects of point mutations on E4 promoter activity;Quantitation of CAT assays...........................................................................81
Figure 2.1.2 D: Sequence differences between pE4CAT andpWE4WT............................................................................................................ 82
Figure 2.1.4 A: Effect of cell type on the requirement for E4F for activation of the E4 promoter by ElA; Representative CATassays..................................................................................................................85
Figure 2.1.4 B: Effect of cell type on the requirement for E4F for activation of the E4 promoter by ElA; Quantitation of CATassays..................................................................................................................86
Figure 3.1.0: A) Binding of ATF-1 to adenovirus early promoters.B) Binding of ATF-2 to adenovirus early promoters..................................97
Figure 3.1.1 A: Effects of point mutations on ATF-1 binding.......................... 99Figure 3.1.1 B: Effects of point mutations on ATF-2 binding..........................100Figure 3.1.2: Stability of ATF-2 DNA binding to point mutated
ATF/E4F binding sites.................................................................................... 103Figure 3.1.3 A: Activation of the IE4xlO series of promoters by an
ATF-2/VP16 fusion protein; Representative CAT assays........................105Figure 3.1.3 B: Activation of the IE4xlO series of promoters by an
ATF-2/VP16 fusion protein; Quantitation of CAT assays....................... 106Figure 3.2: Comparison of the zinc finger motif of ATF-2 with
several representative C2 H2 zinc fingers...................................................... 112Figure 4.1.1: Transcription of the E4 promoter in vitro is inhibited
by the addition of a competitor DNA containing an ATFbinding site..........................................................................................................121
Figure 4.1.2: Analysis of the effect of preincubation of the nuclear extract with the ATF-BS competitor oligonucleotide ondepletion of E4 promoter activity..................................................................122
Figure 4.1.3 A: Effect of glutathione and thrombin on transcriptionof the E4 and MLP promoters in vitro..........................................................124
Figure 4.1.3 B: Effect of imidazole on transcription of the E4 andMLP promoters in vitro...................................................................................125
Figure 4.1.4: Recombinant CREB (H^CRla) restores transcription of the E4 promoter by ATF depleted nuclear extracts, but the CREB dependent stimulation of transcription is not enhanced by protein kinase A (PKA)...............................................................................127
11
Figure 4.1.5 A: Purification of recombinant CREB proteins.A; HéCRla. B; HôCRlAF.................................................................................129
Figure 4.1.5 B: Analysis of the DNA binding activity ofrecombinant CREB proteins............................................................................ 130
Figure 4.1.5: A mutant recombinant CREB protein, H^CRIAP, cannot restore transcription of the E4 promoter by ATF depleted nuclear extracts.................................................................................131
TABLES
Table 1.7.1.3: The relationship between cloned bZIP proteinsbelonging to the ATF family of transcription factors..............................45
Table 1.8.1: Binding specificities of ATF and E4F for binding sitesfrom various adenovirus early promoters................................................53
12
Chapter 1: Introduction.
1.0 General introduction.
The rate and duration of transcription most frequently underlies
the differential rate of protein synthesis in eukaryotes, as it does in
prokaryotes. The relative abundance of a particular RNA transcript
directly reflects the number of translational events occurring. The central
role of transcription in the process of gene expression makes it the most
attractive control point for regulating the expression of genes; it is now
clear that in the vast majority of cases where a particular gene is expressed
only in a particular tissue or in response to a particular signal, this control
is achieved at the transcriptional level (Darnell et ah, 1986).
To begin to unravel such complex events requires the use of
simpler systems that reproduce at least the basic principles of the more
complex regulatory pathways. Researchers began studying adenovirus
gene expression in the early 1970s because it provided a good model
system for the study of eukaryotic gene expression. The adenovirus-
infected cell was considered a particularly attractive system to investigate
because the viral genome is small enough to be analysed in detail (35 kb)
yet large enough that one can expect the expression of the genome to be
regulated during the course of infection. Secondly, adenoviruses such as
type 2 (Ad2) and type 5 (Ad5) were found to be easily propagated in spinner
cultures of HeLa cells and easy to purify. In addition relatively large
quantities of viral RNA are produced during an adenovirus infection.
Thirdly, it was expected that transcription of adenovirus genes would
involve cellular processes and would mimic the host cell in its regulation
of gene expression.
13
In effect the adenovirus genome was likened to a miniature
chromosome and information gathered from the study of adenovirus
gene expression was expected to be generally applicable to gene expression
in eukaryotes. These expectations have to a large extent been fulfilled,
w ith many fundamental discoveries regarding eukaryotic gene
organisation and expression being made as a result of research on the
adenoviruses.
1.1 The adenovirus replication cycle.
Expression of adenovirus genes during a productive infection of
permissive human cells proceeds through two distinct phases, early and
late, which are defined by their punctuation by viral DNA synthesis. This
latter event occurs between 6-9 h after infection under typical conditions of
infection. During the early phase of infection before the onset of viral
DNA replication, several regions of the viral genome are expressed which
give rise to five major classes of mRNA; ElA, ElB, E2A, E3 and E4 (Chow
et ah, 1979; Kitchingman and Westphal, 1980; Nevins et ah, 1979; Shaw
and Ziff, 1980; for review, see; Flint, 1986; see Figure 1.1).
1.1.1 The ElA region.
The first region of the adenovirus genome expressed immediately
following the entry of the viral DNA into the infected cell nucleus is the
E lA region (N evins et ah, 1979; Shaw and Ziff, 1980). RNA
complementary to this region is detected 45 min after infection, with a
maximal rate of RNA synthesis reached after 3 h and maintained
thereafter for at least 6 h (Nevins et ah, 1979). The ElA gene products are a
heterogeneous family of polypeptides that arise from both alternative
14
a i
Proteins
RNAs
13 K 21 K 2 6 K 16 K 32 K 55 K
IX
13.6 K
PTNase
52- penton hexon55 K ilia III pVII V pVI II 23 K
non-viriont *--------\
33 K
14K 14.5 K 11 K 10K
1 2
100 K pVIII 16 K
x y ;
ElAE1B_
fiberIV
L5
L4
L3
L2
L1 E3_ A _
r-strand 3’l-strand 5’
RNAs
[= îVA
I10
I20
I30
I40 50 60
I70
I80 90
3'100
- ]- ]
—Y—E2A
E2B
Proteins IVa2 140 K pol
87 K p-TP
72 K DBP
~E4
11 K 13K 17K 10K 14K(19K .21 K, 2 4 K .3 5 K )
Figure 1.0.2: The Ad2 cytoplasmic RNA transcripts, characterized by electron microscopy of RNAiDNA heteroduplexes. (From Flint, 1986)
splicing of the primary transcript and extensive post-translational
modification of the primary products (Berk and Sharp, 1978; Stephens
et al., 1986). Five different mRNAs from the Ad2 and Ad5 ElA gene have
been identified, having sizes of 9 S, 10 S, 11 S, 12 S and 13 S (Berk and
Sharp, 1978; Chow et ah, 1979; Kitchingman and Westphal, 1980; Stephens
and Harlow, 1987; Ulfendahl etah, 1987; see Figure 1.1.1). The four largest
mRNAs represent differentially processed mRNAs that encode different
portions of a single open reading frame. The most abundant of these are
the 12 S and 13 S transcripts, which are synthesised early in infection and
encode proteins of 243 and 289 amino acids respectively (Perricaudet et ah,
1979). The other products are detectable in the late phase (Stephens and
Harlow, 1987; Ulfendahl etah, 1987) whereas the product of the 9 S
transcript has not yet been found in infected cells (Virtanen and Peterson,
1983).
Viruses carrying mutations that impair or prevent the expression of
E lA gene products have been shown to produce much reduced
concentrations of viral early mRNAs (Berk et ah, 1979; Jones and Shenk,
1979). A similar situation is observed when protein synthesis in cells
infected with the wild type virus is inhibited from the beginning of
infection (Lewis and Mathews, 1980; Shaw and Ziff, 1982). Thus, a product
of the ElA region must be essential for the activation of transcription of all
other transcription units expressed during the early phase of infection.
Subsequent experiments have established that products encoded by the
ElA region greatly stimulate transcription from all of the adenovirus early
promoters, a phenomenon termed trans-activation (for reviews see; Berk,
1986; Flint and Shenk, 1989; Flint, 1986). The mechanisms of this function
of the ElA gene products will be discussed in more detail in a later chapter.
16
500 1000I
1500 J l _
13S 185 aa 104 aa AAAA
12S AAAA139 aa
AAAA87 aa 104 aa
lOS AAAA104 aa41 aa
U S 26 aa
26 aa
104 aa
9 S 126 aah aa ■AAAA
Figure 1.1.1: Structure of transcribed and translated products of the adenovirus ElA region. The structure of the five ElA mRNAs that are synthesized by the alternative splicing of a primary transcript are shown by the relative positions of their coding sequences in the viral genome (numbered). Boxes indicate open reading frames, and dotted lines represent sequences removed during splicing. The shaded box indicates a different reading frame to the C-terminus of the other mRNAs. Erom Dyson and Harlow, (1992).
17
1.1.2 Activation of adenovirus early genes.
Transcription of the other early regions begins slightly later than
that of ElA, starting with the ElB region approximately 60 min after
infection. The E3 and E4 regions are expressed simultaneously at about
90 min after infection and finally the last early region to be expressed is the
E2 region (Nevins et al., 1979). Full activation of E2 transcription is
dependent on the function of a 19 kDa protein encoded by E4 orf6/7 (Cutt
et al., 1987) and this product can function independently of other viral
gene products to frflws-activate the E2A promoter (Hardy et ah, 1989;
Huang and Hearing, 1989). RNA from the E2 region is produced
approximately 2 h after infection, 30 min later than that of the E4 region
(N e v in s etah, 1979). Transcription of all of the early regions then
accelerates during the next few hours of the early phase. The E3 and E4
regions reach a maximal rate of transcription approximately 3 h after
infection, the E2 region 7 h after infection, and the ElB region 8 h after
infection (Nevins et ah, 1979).
The regulation of adenovirus gene expression that is demonstrated
by the above observations is likely to rely upon cellular factors and
presumably reflects cellular modes of regulation. Therefore trans-
activation of the adenovirus early genes by ElA represents an ideal model
system for investigating the mechanisms by which gene expression can be
modulated.
1.2 Mammalian transcription.
Transcription of mammalian genes is performed by three different
RNA polymerases, designated I, II and III (Roeder and Rutter, 1969; Roeder
and Rutter, 1970a; Roeder and Rutter, 1970b). Each polymerase catalyses
18
transcription of genes encoding different classes of RNA. RNA
polymerase I is located in the nucleus and is responsible for synthesis of
ribosomal precursor RNAs. RNA polymerase III functions outside the
nucleus and transcribes the genes for tRNA, 5S rRNA, and an array of
small RNAs. RNA polymerase II is located within the nucleus and
catalyses transcription of all the protein-coding genes; that is, it functions
in the production of mRNAs. In addition, polymerase II also produces
several small RNAs that take part in RNA processing (for review, see;
Adams et al., 1986; Lewis and Burgess, 1982; Sentenac, 1985).
1.3 Control of RNA polymerase II transcription.
The rate of transcription of a gene is controlled by a region of DNA
5' (and occasionally 3') to the transcription initiation site called the
promoter. Mutagenesis studies have demonstrated that the promoters of
genes transcribed by RNA polymerase II usually consist of two functional
elements: A TA-rich region (the TATA box) and one or more upstream
promoter elements (for review, see; Breathnach and Chambon, 1981;
Maniatis et ah, 1987; Mitchell and Tjian, 1989).
1.3.1 The TATA box.
The TATA box is a TA-rich region (consensus TATA^/jA^/t)
located approximately 30 bp upstream from the transcription start site in
many but not all genes (for review, see; Breathnach and Chambon, 1981)
and is the binding site for a 38 kDa protein called the TATA binding
protein (TBP; Hoffmann et ah, 1990; Kao et al, 1990; Peterson et al, 1990).
The interaction between TBP and the TATA box is the first step in the
initiation of transcription, which results in the formation of a stable
(template-committed) and transcriptionally active complex that includes
19
RNA polymerase II (Davison et ah, 1983; Fire et al, 1984; see below).
Deletion or mutation of the TATA box results in a dramatic decrease in
the activity of many promoters in vivo (for example, see; Corden et ah,
1980; Wasylyk and Chambon, 1981) and experiments involving its
relocation have demonstrated that it is an important determinant in the
accurate positioning of the start site of transcription (for example, see;
C orden et ah, 1980). The TATA box is the minimal promoter element
necessary for the initiation of transcription in vitro and appears to be
sufficient to direct a low unregulated (basal) level of transcription (for
reviews, see; Roeder, 1991; Sawadogo, 1990; Weinmann, 1992).
Although most polymerase II promoters contain a consensus TATA
sequence, there are many examples of functional promoter elements that
either contain a TA-rich sequence that varies from the consensus or no
apparent TATA box at all (Breathnach and Chambon, 1981). However, the
yeast TFIID complex has been shown to bind with high affinity to such
non-consensus TATA sequences (Hahn et ah, 1989) and promoters that
lack any homology to the TATA box have been shown to nevertheless
require TBP for the initiation of transcription (Pugh and Tjian, 1991).
Hence the requirement for a consensus TATA box is gene specific and is
most likely dependent upon the presence of either additional promoter
elements such as Spl binding sites (Dynan and Tjian, 1983) or an initiator
element (Smale and Baltimore, 1989). This element was first identified in
some promoters that lack a TATA box and in its absence fixes the start site
of transcription and facilitates the binding of TBP to the promoter
(Carcamo et ah, 1991; Zenzie-Gregory et ah, 1992).
1.3.2 Upstream promoter elements.
Upstream promoter elements are sequences generally between
6 and 20 bp long, often have dyad symmetry and are the binding sites for
20
sequence-specific DNA binding proteins called transcription factors. These
cfs-control elements are located at varying distances upstream of the
TATA box and reversibly enhance or repress the basal level of
transcription (Maniatis et al., 1987; Mitchell and Tjian, 1989).
Upstream promoter elements that are involved in distinct signal
transduction pathways were amongst the first to be extensively
characterised, many of which have since been shown to be present in a
number of cellular and viral promoters. For example, analysis of the
signal transduction pathway activated by cyclic AMP (cAMP) identified an
element in the proenkephalin promoter called the cAMP response
element (CRE; Comb et ah, 1986). Examination of the promoters of many
genes induced by cAMP, such as somatostatin, vasoactive intestinal
polypeptide (VIP), tyrosine hydroxylase and c-fos reveals sequences
homologous to the enkephalin CRE that are limited to a 5 bp TGACG
motif and which are also functionally important for the activation of
those genes by cAMP (Harrington et al, 1987; Montminy and Bilezikjian,
1987; Sassone-Corsi et al., 1988; Tsukada et al., 1987).
1.4 Cellular factors involved in transcription by RNA polymerase II.
1.4.1 RNA polymerase II.
Eukaryotic RNA polymerase II contains two large polypeptide
subunits of 140 and 220 kDa and 10±2 smaller subunits of between 10 and
45 kDa (Armaleo and Gross, 1985; Engelke et al., 1983; Guilfoyle et al., 1984;
Renart et al., 1985; Sentenac, 1985). The two large subunits share several
conserved regions with polymerases I and III (in which 70-80% of the
amino acids are identical) and with the p and p' subunits of the E. coli
RNA polymerase (Allison et al., 1985; Memet et al., 1988; Sweetser et al.,
21
1987). Three of the smaller subunits (14 to 28 kDa) are also found
associated with polymerases I and III, and are hence called common or
shared subunits (Woychik et al., 1990; for review, see; Sawadogo, 1990;
Woychik and Young, 1990; Young, 1991).
The largest subunit of RNA polymerase II contains an unusual
repetitive domain that is not found in the homologous large subunits of
polymerases I and III, or in the E. coli p' subunit (Allison etal., 1985;
Corden et al, 1985). This C-terminal domain (CTD), or tail, consists of a
tandem heptapeptide repeat of the consensus sequence Tyr-Ser-Pro-Thr-
Ser-Pro-Ser. This domain is highly phosphorylated in a substantial portion
of the RNA polymerase II molecules in a mammalian cell and protein
kinases have been purified that appear to be involved in CTD
phosphorylation (Cisek and Corden, 1989; Feaver et ah, 1991; Lee and
Greenleaf, 1989; Stevens and Maupin, 1989). The CTD has been shown to
be essential for initiation from some (but not all) promoters tested, but
does not appear to be required for elongation (for review see; Young, 1991).
Phosphorylation of the CTD appears to occur during initiation and is
postulated to facilitate the transition between promoter binding and RNA
elongating forms of RNA polymerase II (Laybourn and Dahmus, 1989;
Payne et ah, 1989). In addition, several lines of evidence suggest that this
domain is one of several components of the transcription apparatus that
respond to activating signals from factors associated with upstream
promoter elements (Allison and Ingles, 1989; Sigler, 1988; for review, see;
Corden, 1990; Young, 1991).
1.4.2 General transcription (initiation) factors.
The general transcription factors are defined as those factors which
are required for transcription of all Pol II genes. Together with the RNA
22
polymerase, these factors interact with core promoter elements to form a
productive complex that directs a low unregulated (basal) level of
transcription. By reconstituting the transcriptional activity of a promoter
in vitro by the addition of increasingly purified fractions of whole cell or
nuclear extracts, a number of factors required for the initiation of
transcription by RNA polymerase II have been identified. By examining
the properties of these factors the formation of the preinitiation complex is
now well defined and involves the stepwise assembly of several factors,
including RNA polymerase II, into a transcriptionally active initiation
complex (for reviews, see; Roeder, 1991; Sawadogo, 1990; Weinmann, 1992;
see Figure 1.4.2.2).
1.4.2.1 The TATA binding protein.
The TATA binding protein (TBP) is a 38 kDa protein that binds to
the TATA consensus sequence motif (Peterson et al, 1990) and is tightly
associated with several other polypeptides (TBP associated factors, or
TAFs) to form the multiprotein complex TFIID (Dynlacht e t a l , 1991;
Tanese e ta l , 1991). TBP cannot substitute for native TFIID in activator
dependent transcription, but can mediate preinitiation complex assembly
and basal (core promoter) transcription in concert with other general
initiation factors (Peterson et al, 1990). It interacts directly with the general
initiation factors TFIIA and TFIIB, the C-terminus of the large subunit of
RNA polymerase II and an initiator binding factor (TFII-I) that may be
important for transcription from TATA-less promoters (Carcamo et a l ,
1991; Smale e t a l , 1990; Zenzie-Gregory e t a l , 1992; for review, see;
Weinmann, 1992; see Figure 1.4.2.2).
23
T fllD V______ /
TATACAP
TFIIA I J TFIIB
POL II7777TFIIDTFIIA TFIIB
T F I H
POL II
TFIIA I J TFIIB
POL IITFIIDTFIIA TFIIB
CAP
Figure 1.4.2.2: Schematic representation of the transcriptional initiation of the adenovirus major late promoter. The diagram summarises the order of events that lead to the formation of a functional intiation complex and does not represent the position, size or shape of the complex at the promoter. Adapted from Weinmann (1992).
24
1.4.2.2 Other factors involved in assembly of the preinitiation complex.
The first step in the assembly of the preinitiation complex involves
the binding of the TFIID complex (which includes TBP; see above) to the
TATA box (Davison et al , 1983; Fire e ta l , 1984; see Figure 1.4.2.2). This
interaction results in a template-committed complex (a stable complex
resistant to challenge by an equivalent protein-free template; Davison
et a l , 1983; Fire et ah, 1984) which nucleates the ordered assembly of the
other general transcription factors TFIIA, TFIIB, TFIIE and TFIIF, together
with RNA polymerase II, into an active transcription complex (Buratoski
et ah, 1989; Flores et ah, 1988; Inostroza et ah, 1991; Maldonado et ah, 1990;
for review, see; Roeder, 1991; Weinmann, 1992 ; summarised in
Figure 1.4.2.2). Subsequent events involving formation of the initiation
complex and leading to RNA elongation are less well defined, but may
involve at least another four activities (TFIIG (Sumimoto et ah, 1990),
TFIIH and TFIIJ (Flores et ah, 1992) and TFIIS (Reinberg and Roeder, 1987).
1.4.3 Gene-specific transcription factors.
Transcription factors that bind to upstream promoter elements are
gene-specific; that is they are involved in the selective activation of
particular genes through their exclusive interaction with a small subset of
these short DNA elements (Dynan and Tjian, 1985; Mitchell and Tjian,
1989). Particular gene-specific transcription factors are involved in a
variety of regulatory pathways, including tissue-specificity of gene
expression (for example, the lymphoid cell-specific expression of
immunoglobulin genes; Staudt et ah, 1986; Lenardo et ah, 1987; Pierce
et ah, 1988) and cellular response to outside stimuli such as heat-shock
(Pelham, 1982; Kingston et ah, 1987) or hormones (Miesfeld et ah, 1984).
25
Many transcription factors have now been cloned and analysis of
their cDNAs has revealed several structural features common to this
category of proteins. Typically, they can be dissected into two functional
regions; a domain responsible for sequence-specific DNA binding and in
many cases dimérisation; and one or several discrete domains involved in
transcriptional activation (for review, see; Mitchell and Tjian, 1989).
Studies of the yeast activator protein Gal4 have demonstrated that this
modular structure is remarkably flexible, with domains from different
proteins generally being inter-changeable with those of Gal4 with no
apparent loss of function (Brent and Ptashne, 1985; Keegan et al., 1986).
Many transcription factors bind to DNA as dimers and employ a
limited number of structural motifs to achieve dimérisation and DNA
binding. As a result, they are often classified according to which motifs
com pose the dimérisation and /or DNA binding domain. DNA
recognition motifs include the basic domain (Johnson et ah, 1987;
Landschulz et at., 1988) and zinc fingers (Miller et ah, 1985). Dimérisation
regions include the leucine zipper (Landschulz et ah, 1988) and
helix-loop-helix motifs (Steitz et ah, 1982) and both of these regions often
adjoin a basic (DNA recognition) domain (for review, see; Busch and P.,
1990; Struhl, 1989).
The activation domains of transcription factors are presently
classified according to their amino acid composition, and regions that are
rich in acid amino acids {e.g. VP16 (Triezenberg et ah, 1988)), proline
{e.g. CTF/NFI (Mermod et ah, 1989)) or glutamine {e.g. SPl (Courey and
Tjian, 1988)) have been identified. The best studied example of an
activation domain is the acidic activating domain of the herpes simplex
virus VP16 protein (Triezenberg et ah, 1988). Mutagenesis experiments
have confirmed that the acidic nature of VP16 is important for its
potential to stimulate transcription, but that several bulky hydrophobic
26
residues {e.g. Leu, lie, Phe) in the activation domain are required
absolutely (Cress and Triezenberg, 1991). The acidic activation domain of
VP16 has been shown to directly interact with the general transcription
factors TFIID (Stringer et al., 1990) and TFIIB (Lin et al, 1991) and these
interactions are not simply ionic in nature. Mutations of a phenylalanine
residue in the activation domain that have no net effect on the charge of
the region but which eliminate the activation function (Cress and
Triezenberg, 1991) are detrimental to the interaction between VP16 and
either TFIIB (Lin et a l , 1991) or TFIID (Ingles et a l , 1991). Hence these
results are interpreted to infer a direct mechanism for the activation of
transcription by acidic domains like that of VP16.
Other reports have suggested a role in activated transcription for a
class of non-DNA binding proteins that are neither transcriptional
activators nor general factors (Berger et al, 1990; Hoey et al, 1990; Kelleher
et a l , 1990; Pugh and Tjian, 1990). These novel molecules, variously
termed adaptors, coactivators, or mediators, are envisioned to bridge or
stabilise interactions between activators bound at upstream promoter
elements and the general factors bound at the TATA box (Pugh and Tjian,
1990; for review, see; Gill and Tjian, 1992).
1.5 Trflws-activation by adenovirus El A proteins.
Adenovirus ElA proteins represent another class of transcriptional
activators that neither bind DNA in a sequence-specific manner nor target
a unique promoter element in any other way; a property shared by several
other viral frflws-activating proteins {e.g. pseudorabies virus immediate
early proteins (Abmayr et al, 1985); HTLV-1 p40* (Fujisawa et a l , 1989);
HBV X gene product (Maguire et al, 1991).
27
The first indication that ElA regulates the expression of the early
adenovirus genes came from the analysis of mutant ElA viruses. Viruses
whose E lA regions are deleted, or more subtly mutated, support the
efficient expression of no regions of the viral genome other than ElA
itself, when present (Berk et ah, 1979; Jones and Shenk, 1979). Subsequent
experiments have established that this control is at the level of
transcription and that ElA acts in trans on the viral promoters (Flint,
1986). Trflns-activation by E lA is not limited to adenovirus genes;
transcription of some cellular genes ((3-globin and preproinsulin) is
stimulated when plasmid clones of the genes are co-transfected into HeLa
cells with a plasmid expressing ElA (Gaynor et ah, 1984; Green et ah, 1983).
Curiously, the endogenous counterparts of these cellular genes are not
activated (Gaynor et ah, 1984; Svensson and Akusjarvi, 1984) and other
experiments have concluded that transcription of most endogenous genes
is not induced by ElA (Beltz and Flint, 1979). However, it does appear that
ElA can stimulate transcription from the promoters of at least two
endogenous cellular genes, p-tubulin (Stein and Ziff, 1984) and hsp70 (Kao
and Nevins, 1983). The diversity of ElA's frans-activating properties is
further emphasised by its ability to stimulate transcription by RNA
polymerase III (Gaynor et ah, 1985; Hoeffler and Roeder, 1985).
In addition to activating transcription, ElA proteins have also been
demonstrated to effectively repress SV40 or polyoma virus enhancer-
mediated transcription (Borrelli et ah, 1984; Velich and Ziff, 1985). The
enhancers negatively regulated by ElA include not only those that are
active in a wide variety of cells, but also cell-specific enhancers, such as the
mouse immunoglobulin heavy and light chain enhancers, active only in
lymphoid IB cells (Hen et ah, 1985). In contrast, the same enhancer of the
mouse immunoglobulin heavy chain gene is activated by E lA in
nonlymphoid cells (Borrelli et ah, 1986).
28
12SmRNA
13SmRNA
sssssssCRl CR2
im ECRl CR2 CR3
243 aa Phosphoprotein
289 aaPhosphoprotein
6
CRl CR2 CR313S ElA 289 aa
139 154 157 171 174 188
Figure 1.5: General structure of the products of the adenovirus ElA region. A; Structure of the 12S and 13S mRNAs and their protein products. B; Structure of CR3 of the large ElA protein. The encircled Cs denote the cysteine residues that form the zinc finger motif, chelating a single Zinc ion (Zn). Numbers below the figure denote the amino acid position in ElA.
29
How the ElA protein is able to accomplish these diverse forms of
transcriptional control is not clear. The promoters activated by ElA do not
share one common czs-acting DNA sequence motif and consistent with
this lack of sequence specificity in function, ElA proteins have only a very
low affinity for DNA and no apparent sequence specificity (Chatterjee
et al., 1988; Zu et ah, 1992). In addition, mutation or deletion of the region
of ElA responsible for this weak DNA binding activity has no effect on the
ability of ElA to frans-activate the adenovirus E4 promoter (Zu et al.,
1992). Systematic mutagenesis of the adenovirus early promoters has not
revealed promoter sequences required exclusively for ElA trans-activation
(Berk, 1986). Instead, the promoter sequences required for the maximal
level of ElA activated transcription appear to be also required for the
maximal basal level of transcription in the absence of ElA (Berk, 1986).
Such observations have led to the conclusion that ElA acts by a
mechanism that must be both indirect and general.
1.5.1 A unique conserved region (CR3) of the large ElA protein is essential
for trans-activation by ElA.
During the early phase of a productive adenovirus infection, the
ElA region gives rise to two major, differentially spliced mRNAs (Berk
and Sharp, 1978; see Figure 1.5 A). These 12 S and 13 S mRNAs encode two
closely related nuclear-localised phospho-proteins of 243 and 289 amino
acids respectively, that differ only in an internal 46 amino acid region that
is unique to the larger ElA protein (Perricaudet et al, 1979; see Figure 1.5 A
and B). This region is one of three regions that are conserved among
distantly related human adenoviruses and has been termed conserved
region 3, or CR3 (Kimelman et al., 1985; van Ormondt et al , 1980).
Trans-activation of the early viral promoters was found to be an
activity of the product of the 13 S mRNA but not the 12 S mRNA (Carlock
30
and Jones, 1981; Esche et ah, 1980; Glenn and Ricciardi, 1985; Graham et ah,
1978; Lillie et ah, 1986; Montell et ah, 1982; Ricciardi et ah, 1981) and several
mutational studies have confirmed the importance of the unique region
of the 289 amino acid ElA protein (CR3) to its frans-activation activity.
Mutations in other regions of the large ElA protein, including the two
other conserved regions (CRl and CR2), have little or no effect on the
frflns-activating activity of the large ElA protein, whereas mutations in
CR3 have been found to severely impair frans-activation (Glenn and
Ricciardi, 1985; Lillie et ah, 1986; Moran et ah, 1986; for review, see; Moran
and Mathews, 1987).
However, some evidence for transcriptional activation by products
of the ElA 12 S mRNA has been reported. Although most viruses
expressing only the 12 S mRNA grow poorly on HeLa cells (Haley et ah,
1984; Montell et ah, 1984) the cDNA virus dl347 grows well and induces
the synthesis of larger quantities of early mRNA species than does the
virus dl312 (whose ElA region has been completely deleted), although
resulting in a fivefold reduced yield of virus compared to wild type
infection (Winberg and Shenk, 1984). The 12 S ElA mRNA product has
also been observed to stimulate transcription of the E2 and E3 regions
when plasmids containing these genes are co-transfected into HeLa cells,
although less efficiently than the ElA 13 S mRNA products (Leff et ah,
1984). In addition, the 243 amino acid ElA protein produced in Escherichia
coli can complement dl312, although to a lesser extent than the bacterially
produced 289 amino acid protein (Ferguson et ah, 1985). Hence it seems
clear that activation of early viral genes during a productive infection is
largely a function of the 289 amino acid ElA protein, although the 243
amino acid E lA protein can activate transcription under certain
circumstances.
31
1.5.2 CR3 contains one copy of a C4 zinc finger motif that is essential and
sufficient for trans-activation.
Four conserved cysteine residues of CR3 constitute one copy of a
motif that bears homology to a C4 zinc finger (see Figure 1.5 B). The 289
amino acid ElA protein binds one mole Zn^+/mole protein and point
mutations in the conserved cysteines that form the zinc finger prevent
zinc binding and trans-activation (Culp et ah, 1988), indicating that the
integrity of this domain is important to both of these processes.
Remarkably, a 49 amino acid synthetic peptide that comprises the 46
amino acids of CR3, plus three adjacent and conserved amino acids, is able
to stimulate transcription from the adenovirus E2A promoter in vivo
(Green et ah, 1988; Lillie et ah, 1987; Pusztai et ah, 1989) and from the MLR
and E3 promoters in vitro (Green et ah, 1988; Loewenstein and Green,
1989). Mutational analysis of this peptide has demonstrated that the
conserved cysteine residues present in CR3 are essential for its activity
(Green et ah, 1988; Loewenstein and Green, 1989).
1.5.3 CR3 consists of two subdomains.
Further analysis of the function of both the zinc finger and adjacent
regions of CR3 has been performed utilising transient expression assays.
These involve the co-transfection of a test promoter with a plasmid
expressing the ElA gene and enable a quantitative estimation of the effects
of mutations in these regions. Lillie and Green (1989) demonstrated that a
chimeric protein, consisting of the DNA binding domain of the yeast
activator Gal4 fused to amino acids 121-223 of ElA, can activate
transcription from a promoter containing Gal4 binding sites following
their co-transfection into CHO cells. In contrast, a Gal4-E1A fusion protein
containing amino acids 150-223 of ElA cannot activate transcription, but
this activity can be rescued by the addition of the VP16 acidic activating
32
domain to the fusion protein (Lillie and Green, 1989). These results
demonstrate that ElA behaves in a similar manner to a promoter bound
activator and suggest that the region between amino acids 121-150 of ElA,
which includes the N-terminus of CR3, includes an activating domain.
Utilising a similar assay, Martin et al. (1990) found that the
boundaries of the ElA activating domain coincide with the N-terminus of
CR3 and the C-terminus of the zinc finger, and characterised point
mutations in this region that reduce or eliminate the activating function
of ElA. In addition, a Gal4-E1A fusion protein was found to inhibit
activation of a promoter containing LexA binding sites by a LexA-ElA
fusion protein. This inhibition can be removed by the same mutations in
Gal4-E1A that affect activation of the promoter containing Gal4 binding
sites (Martin et ah, 1990). Hence ElA is postulated to contain an activating
domain that directly interacts with a component of the transcriptional
machinery and inhibition by an heterologous ElA fusion protein is likely
to be due to the specific sequestering (or squelching) of this cellular target
(Martin et al., 1990).
Like the wild type ElA protein, the Gal4-E1A fusion protein also
efficiently activates the E4 promoter in this assay (Lillie and Green, 1989).
However, a second class of mutation has been characterised that destroys
the ability of Gal4-E1A to activate the E4 promoter, but which does not
affect activation of the core promoter containing Gal4 binding sites (Lillie
and Green, 1989). In contrast to the effects of mutations in the activating
domain, the effects of these mutations cannot be rescued by the addition to
the fusion protein of the VP16 acidic activating domain. These mutations
include a non-conserved point mutation in the C-terminal region of CR3
at amino acid 180, which further distinguishes this domain from the
activating domain which is localised in the N-terminal region of CR3.
These observations are consistent with this domain being required for the
33
targeting of ElA to the promoter, and taken together with the above
results, ElA is suggested to be able to function in the vicinity of the
promoter by interacting with a factor(s) already bound to it.
These results are mirrored by the observation that mutations in the
postulated promoter targeting domain of ElA exhibit a negative trans-
dominant phenotype over the wild type protein (Glenn and Ricciardi,
1985; Glenn and Ricciardi, 1987; Webster and Ricciardi, 1991). Webster and
Ricciardi (1991) performed an extensive mutational analysis of CR3 and
defined several mutations in ElA that are defective in their trans-
activation of the adenovirus E3 promoter in vivo as determined by
co-transfection in a transient expression assay. These mutant proteins
were also tested for their ability to inhibit trans-activation by the wild type
protein upon their co-transfection, an effect termed trans-dom inance.
Trans-dominant mutations were found to map to a discrete region
C-terminal to the zinc finger (Webster and Ricciardi, 1991) that
corresponds to the promoter targeting domain described above (Lillie and
Green, 1989; Martin et al, 1990). Deletion of this region produces a very
strong trans-dominant mutation, indicating that the activating domain
alone is required for trans-dominance (Webster and Ricciardi, 1991).
In contrast to mutations in the promoter targeting domain,
mutations in the activating domain and zinc finger motif of ElA do not
exhibit trans-dominance. In addition, every mutation that destroys trans
activation without displaying trans-dominance, when introduced as a
second-site substitution into a trans-dominant mutant, destroys the trans
dominant phenotype (Webster and Ricciardi, 1991). The phenotypic
disparity between the activating domain and the promoter targeting
domain mutants indicates that they are distinct functional domains
within the trans-activating domain of ElA. In agreement with the
hypothesis described by Martin et al. (1990), the results of Webster and
34
Ricciardi (1991) strongly imply that the activating domain of ElA binds a
limiting cellular factor (such as a general initiation factor or an adaptor
protein) and are consistent with the suggestion that the promoter targeting
domain of ElA serves to localise ElA in the vicinity of the promoter by
interacting with a factor already bound to it.
1.5.4 The activating domain of ElA specifically interacts with TBP.
The suggestion that the activating domain of ElA might interact
with a component of the transcription complex is supported by
observations that certain TATA box sequences can mediate activation by
E lA (Simon et ah, 1988; Wu et ah, 1987), and that adenovirus infection
increases the transcriptional activity of a partially purified TFIID fraction
from HeLa cells (Leong et ah, 1988). Lee et al. (1991) have further
demonstrated that the large ElA protein binds specifically and stably to the
human TATA binding protein (TBP) using immunoprécipitation and far
western blotting analysis. In addition, using sedimentation velocity
analysis, Lee et ah (1991) demonstrated that these two proteins form a
discrete complex that is consistent with a one-to-one complex of the two
proteins {i.e., a heterodimer) and which appears to contain no other
proteins other than ElA and TBP (Lee et ah, 1991). The 243 amino acid ElA
protein, which is a much poorer trans-activator (for review, see; Flint and
Shenk, 1989), also binds to TBP although much more weakly than the
large ElA protein (Lee et ah, 1991). The interaction between the large ElA
protein and TBP is dependent upon the integrity of the activating domain
of ElA, since deletion of part of the activation domain (AN149; Martin
et ah, 1990) or two mutations in the activating domain that impair trans-
activation (148E^G and 151G—>A; Martin et ah, 1990; Webster and
Ricciardi, 1991) also diminish binding to TBP (Lee et ah, 1991). However,
another mutation in the activation domain that impairs trans-activation
35
(145D^A; Martin et al, 1990; Webster and Ricciardi, 1991) has no effect on
binding of TBP. Hence the ability of ElA to bind to TBP would appear to be
necessary but not sufficient for trans-activation by ElA.
1.5.5 ElA can activate transcription in vitro.
Several studies have examined the ElA dependent activation of
adenovirus genes in vitro. Leong and Berk (1986) used a run-off
transcription assay with whole cell extracts (Manley et al., 1980) to analyse
the activity of the major late promoter (MLP) in vitro (Leong and Berk,
1986). Extracts from adenovirus infected cells were found to be 5-15 times
more active in transcribing the MLP than extracts from mock-infected
cells, or cells infected with viruses that do not express the 13 S ElA
mRNA. Similar results were observed for transcription from the protein
IX and E3 promoters, whereas a modest 2-fold increase was observed for
the human p-globin promoter (Leong and Berk, 1986).
Spangler et al. (1987) used a similar run-off transcription assay with
whole cell extracts (Manley et al., 1980) to analyse transcription of the E2A
and MLP promoters in the presence and absence of a bacterially expressed
large ElA protein (Spangler et al., 1987). Transcription from the E2A
promoter was stimulated up to 7-fold following addition of the 289 amino
acid protein, compared to only 2-fold stimulation of MLP (Spangler et al.,
1987). Green et al. tested a 49 amino acid synthetic peptide (PD3)
comprising the 46 amino acids of CR3 plus three adjacent and conserved
amino acids for its ability to stimulate transcription of the major late
promoter in vitro. Using a run off transcription assay with nuclear extracts
prepared as described by Dignam et al. (1983) the major late promoter was
stimulated up to 20-fold following addition of PD3, whereas peptides
containing mutations in the conserved cysteine residues of CR3 were
found to be totally inactive (Green et al, 1988). This peptide was also found
36
to stimulate transcription from the E3 promoter (Loewenstein and Green,
1989) but not from several other cellular promoters (Green et ah, 1988;
Loewenstein and Green, 1989). Finally, a bacterially produced 289 amino
acid ElA protein has also been shown to stimulate transcription from the
RNA polymerase III transcribed adenovirus virus-associated (VAl) RNA
gene in vitro, using whole cell extracts from HeLa cells (Datta et at., 1991).
1.6 Characterisation of the adenovirus E4 promoter.
All of the early viral promoters have been analysed extensively and
have been found to be similar in structure to typical cellular promoters
that direct transcription by RNA polymerase II, consisting of a TATA box
and one or more upstream promoter elements. Several experimental
approaches have led to the characterisation of upstream promoter
elements that contribute to E4 promoter activity and hence could
potentially be involved in its trans-activation by ElA. Deletional analysis
has identified regions of the promoter that are important for its activity
in vitro and in vivo. DNAse I footprint and méthylation interference
assays have identified binding sites for cellular transcription factors, some
of which overlap or coincide with functionally defined regions. DNA
binding assays and in vitro transcription assays have been used to further
characterise the cellular factors that interact with these elements and
cDNAs encoding some of these proteins have been isolated.
1.6.1 Deletional analysis of the E4 promoter.
The effects of deletions of the E4 promoter upon its activity in vivo
have been extensively studied using transient transfection assays. Gilardi
and Perricaudet (1984) examined the effect of progressive deletion into the
37
upstream region of the E4 promoter. Little transcription was detected from
the E4 promoter when it was transfected into HeLa cells, whereas over
twenty fold more transcription was detected when it was co-transfected
with a plasmid encoding the large ElA protein (Gilardi and Perricaudet,
1984). Deletion of E4 promoter sequences up to -241 has little effect on
transcription (Gilardi and Perricaudet, 1984) and hence the NFl binding
site and two homologies to the SPl binding site (present between -330 and
-241; see Figure 2.1.0 A) are not required for activation of the E4 promoter.
Further deletion to -220 results in a reduction of E4 transcription to 30% of
the wild type level (Gilardi and Perricaudet, 1984) and this deletion
removes another potential SPl binding site. Deletion to -179 has no
further effect on transcription, but deletion to -158 reduces promoter
activity to less than 5% of the wild type level (Gilardi and Perricaudet,
1984). The region between -179 and -158 includes one half of a 13 bp
inverted repeat (between -172 and -160; the complement lies between -54
and -42) and a 10 bp homology to the core sequence of an enhancer
element in the ElA promoter (Hearing and Shenk, 1983).
Lee and Green (1987) showed that a region of the promoter located
from -100 to -200 can function as an ElA dependent enhancer element.
Using a transient transfection assay, there was found to be little effect of
deleting the promoter to -200. In the presence of ElA, further deletion of
the E4 promoter to -138 resulted in a 30 fold decrease in transcription,
whereas in the absence of ElA there was no effect of this deletion. When
E4 sequences from -100 to -200 were placed 600 bp upstream of the E4
TATA box, this region could still activate transcription to normal levels,
and its effect was found to be orientation independent. In addition, this
region was shown to stimulate transcription of heterologous promoters in
the presence of ElA in a similar orientation and distance independent
manner.
38
1.6.2 Identification of cellular factor binding sites within the E4 promoter.
Footprinting experiments have demonstrated the presence of
several binding sites for cellular proteins within the E4 promoter, some of
which lie within the designated enhancer region. Lee and Green (1987)
observed footprints with crude nuclear extracts in three distinct regions of
the promoter, between positions -135 to -172; -39 to -56; and around -110
(see Figure 1.6.2). The -135/-1 72 region was shown to consist of two
overlapping sites; a site distal to the TATA box (positions -150 to -172) is
protected at low protein concentrations whereas a site proximal to the
TATA box (positions -135 to -150) is only protected at high protein
concentrations. The distal site (-150/-172) and the region adjacent to the
TATA box (-39/-56) include the 13 bp inverted repeat sequences and
competition experiments demonstrate that they bind a common factor(s)
(Lee and Green, 1987). These two sites have a 5 bp motif (TGACG) in
common with the -135/-150 site and two other sites observed by Watanabe
et al. (1988) at positions -221 to -245 and -253 to -273 (see Figure 1.6.2). This
5 bp motif is also present in the adenovirus ElA, E2A and E3 promoters, as
well as several other viral and cellular promoters, suggesting that these
promoters could be activated by a common mechanism.
Raychaudhuri et al. (1987) analysed interactions between cellular
proteins and the E4 promoter utilising fractionated cell extracts.
Adenovirus infected extracts were fractionated by heparin-agarose
chromatography and fractions were tested for their ability to bind to the
E4 promoter. Fractions that yielded well resolved and reproducible
interactions with the promoter were examined further by footprint
39
-250
TATGATAATGAGGGGGTGGAGTTTGTGACGTGGCGCGGGGCGTGGGAACGGGGCGGGATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCACCCTTGCCCCGCCC
-200
TGACGTAGGTTTTAGGGCGGAGTAACTTGTATGTGTTGGGAATTGTAGTTTTCTTACTGCATCCAAAATCCCGCCTCATTGAACATACACAACCCTTAACATCAAAAGAA
-150 • • • * * * # # " " 'AAAATGGGAAGTTACGTCACGTGGGAAAACGGAAGTGACGATTTGAGGAAGTTGTTTTTACCCTTCAATGCAGTGCACfCCTTTTGCCTTCACTGCTAAACTCCTTCAACA
-100
GGGTTTTTTGGCTTTCGTTTCTGGGCGTAGGTTCGCGTGCGGTTTTCTGGGTGTTTCCCAAAAAACCGAAAGCAAAGACCCGCATCCAAGCGCACGCCAAAAGACCCACAAA
-50
TTTGTGGACTTTAACCGTTACGTCATTTTTTAGTCCTATATATACTCGCTCTGCAAACACCTGAAATTGGCAATGCAGTAAAAAATCAGGATATATATGAGCGAGACG
Figure 1 .6 .2 : Summary of results from DNasel footprinting (from Watanabe et aL, 1988) and méthylation interference assay analysis (from Raychaudhuri et al., 1987) of the adenovirus E4 promoter. Regions that bind cellular proteins (as determined by DNasel footprinting) are indicated by black lines on each DNA strand. The thicker lines indicate footprints which include the 13 bp imperfect inverted repeat, and the thinner lines the footprint at -110 also observed by Lee and Green (1987). Residues that are directly involved in DNA/protein interactions (as determined by méthylation interference assays) are indicated by black dots. The TGACG (CGTCA) motifs and the TATA box are shown in bold type. The dashed lines indicate the E4F2 (at -150; Hai et al., 1988) and the E4TF1 (at -140; Watanabe et al., 1988) binding sites.
40
analysis to determine which sequences were protected by each heparin-
agarose fraction.
This approach demonstrated the presence of several interactions
between cellular proteins and the E4 promoter. A single factor was shown
to interact with the -39/-56 and -150/-172 sites, and was termed E4F (see
below). Other interactions were observed at positions -101, -149 to -150, -156
to -158 and -173 to -175 (Raychaudhuri et al, 1987; see Figure 1.6.2). The
interaction at position - 1 0 1 may correspond to the footprint observed with
crude cell extracts around position -110 (Lee and Green, 1987; Watanabe
et al., 1988); the interaction at -149/-150 is within the binding site of the
E4TF1 factor (Watanabe et al, 1988) and one of the homologies to the ElA
core-enhancer (Hearing and Shenk, 1983); the interaction at -156/-158
corresponds to the binding site of the E4F2 factor (Hai et al, 1988b); and
finally the interaction at -173/-1 75 is within another of the homologies to
the ElA core enhancer.
Finally, although a factor can associate with the region around
position -1 1 0 , this region can be deleted or replaced without affecting
transcription in vivo or in vitro (Handa and Sharp, 1984).
1.7 Characterisation of proteins that interact with the E4 promoter.
1.7.1 The ATF family.
1.7.1.1 Characterisation of ATF binding sites in the viral early promoters.
Lee and Green (1987) demonstrated that a single factor (or related
factors) binds to both the -150/-172 and -39/-56 sites of the E4 promoter (see
Figure 1.6.2), since footprints observed at these sites could be removed by
competition with DNA containing either site. In addition, they also
showed that the factor binding to these sites in the E4 promoter is the
41
same as (or related to) a factor binding to the E2A promoter, since
footprints at the sites in the E4 promoter can be removed by competition
with DNA containing E2A promoter sequences.
Analysis of the E2A and E3 promoters demonstrated that each of
these promoters contains a binding site for a cellular factor which includes
the 5 bp TGACG motif present in the E4 promoter. SivaRaman et al. (1986)
analysed linker scanning mutants of the E2A promoter utilising DNA
binding and footprinting assays, and demonstrated that a 17 bp sequence in
the E2A promoter, which includes the TGACG motif, is the binding site
for a cellular factor. This factor was also demonstrated to bind to the ElA,
E3 and E4 promoters, since DNA fragments containing these promoters
compete for binding of this factor to the E2A-probe DNA. Similar studies
of the E3 promoter (Hurst and Jones, 1987) demonstrated the presence of
three binding sites for cellular factors within this promoter, one of which
(BS2) includes the TGACG motif. BS2 competitor DNA was shown to
compete for binding of factors to an E2A probe, whereas competitor DNAs
containing the other E3 sites cannot. This demonstrated that the E3 BS2
site binds either the same (or a related) factor as the E2A promoter.
Both of these studies provided further evidence that the TGACG
sequence in each of the E2A, E3 and E4 promoters comprises the core motif
of the binding site for the same cellular factor. Lee et al. (1987) performed
extensive DNA binding assays with probes from each of the E2A, E3 and
E4 promoters and competitor DNAs to each of the characterised sites, and
demonstrated that the same (or related) factors bind to each of the sites
containing the TGACG motif. This factor has been termed activating
transcription factor (ATF; Lee et al., 1987a). These experiments were seen
as further evidence that these promoter may share a common mechanism
for activation by ElA.
42
1.7.1.2 Isolation of ATF proteins.
Gel mobility shift assays demonstrate that multiple factors bind
directly to ATF binding sites, and sequence-specific DNA affinity
chromatography has enabled the purification of some of these binding
activities. One family of immunologically related phosphoproteins of -43
and 47 kDa (referred to as ATF-43 and ATF-47, respectively) were purified
from HeLa cells and characterised by their ability to bind to ATF binding
sites present in the viral early promoters (Hai et ah, 1988b). A factor of
similar size, designated CREB (cAMP response element binding protein),
was purified from PC12 cells (Montminy and Bilezikjian, 1987) and rat
brain (Yamamoto et al., 1988) and characterised by its ability to bind to the
CRE (cAMP response element) present in the rat somatostatin promoter
(Montminy and Bilezikjian, 1987).
1.7.1.3 cDNA cloning reveals a multigene family of transcription factors.
Subsequent cloning of CREB revealed that it is a member of the
bZIP family of transcription factors (Gonzalez et ah, 1989; Hoeffler et ah,
1988a) and further analysis demonstrated that it corresponds to ATF-47
(Hurst et ah, 1990). Further cDNA cloning studies demonstrated that an
even more complex array of factors can interact with ATF binding sites.
Hai et ah (1989) isolated several cDNAs that encode distinct proteins which
bind to ATF binding sites. Like CREB, all of these proteins were found to
belong to the bZIP family of transcription factors, indicating that
collectively they belong to a multigene family (the ATF family).
Eight partial cDNAs were isolated (ATF-1 to -8 ) which bind with
varying efficiency to ATF binding sites. Of these, five clones (ATF-1,
ATF-2, ATF-3, ATF-4 and ATF-7) bind efficiently to a single ATF binding
43
acid basic RNR A2 -3 KCR basic spacer L L N L L L
CREB E E A A .R KREVRLM KNR EAAR ECR RKKK EYVKC L ENRVAV L ENQ N KT L lE EL K A LATF-1 TDDPQL KREIRLM KNR E A .R ECR RKKK EYVKC L ENRVAV L ENQ N KT L lE E L K T LATF-2 NEDPDE KRRKFLE RNR AAAS RCR QKRK VWVQS L EKKAED L SSL N GQ L QSEVTL L RNEVAQ LATF-3 APEEDE RKKRRRE RNK lA A A KCR NKKK EKTEC L QKESEK L ESV N AE L KAQLEE L KNEKQH LATF-4 KGEKLD KKLKKME QNK TAAT RYR QKKR AEQEA L TGECKE L EKK N EA L KERADS L AKEIQY LATF-5 ISRRRR EKENPKE RNK MAAA KCR NRRR ELTDT L QAETDQ L EDE K SA L QTELAN L LKEKEK LATF- 6 SDIAVL RRQQRMI KNR ESAC QSR KKKK EYMLG L EARLKA A LSE N EQ L KKENGR L KRQLDE LATF-a DEDPDE RRQRFLE RNR AAAS RCR QKRK LWVSS L EKKAEE L TSQ N IQ L SNEVTL L RNEVAQ Lc-JUN ESQ ERI KAERKRM RNR lA A S KCR KRKL ERIAR L EEKVKT L KAQ N SE L ASTANM L REQVAQ Lc-FOS E . . E . . KRRIRRE RNK MAAA KCR NRRR ELTDT L QAETDQ L EDE K SA L QTEIAN L LKEKEK LhXBP HLSPEE KALRRKL KNR VAAQ TAR DRKK ARMSE L EQQWD L EEE N QK L LLENQL L REKTHG LE4BP4 DEKKDA MYWEKRR KNN EAAK RSR EKRR LNDLV L ENKLIA L GEE N AT L KAELLS L KLKFGL IC/EBP VDKNSN EYRVRRE RNN lA V R KSR DKAK QRNVE T QQKVLE L TSD N DR L RKRVEQ L SRELDT L
W ENQ E
Figure 1.7.1.3: Amino acid homology in the basic domain and leucine zipper regions of several members of the ATF family and selected other bZIP proteins that are known to interact with the E4 promoter. Regions of homology are boxed, with conserved residues in bold. See Table 1.7.1.3 for references.
Equivalent (=) and like (-) proteins:
CREBi'2 = ATF-472.3; ~ CREM^ - ATF-l^ATF-15,6 = ATF-433'6 = TREB367; - CREB - CREM^ATF-25 = CREBPl» = mXBPl^ = TREBT = HB16^0; - ATF-aii ATF-45 = TREB6712 = CREB213; ~ mATF-4:4 ATF-55 - Pos:5 hXBPi6 = TREB57
Table 1.7.1.3: The relationship between purified and cloned bZIP proteins belonging to the ATF family of transcription factors. Several bZIP proteins that bind to the ATF consensus sequence have been cloned by more than one group and are hence referred to by more than one name. Above is a brief summary of the relationships between these proteins, adopting the notation according to Hai et a l (1988) as the standard.
References: ^Hoeffler etal . (1988a); Gonzalez etal . (1989); 2 Hurst et al. (1990); ^Hai et al. (1988b); Hurst and Jones (1987); ^Foulkes et al. (1991); ^Hai et al. (1989); 6 Hurst et al. (1991); ^Yoshimura et al. (1990); ^Maekawa et al. (1989); ^Ivashkiv et al. (1990); ^ Kara et al. (1990); ^^Gaire et al. (1990); i2Tsujimoto etal. (1991); ^^willems etal. (1992); ^^Mielnicki and Pruitt (1991); i^van Straaten et al. (1983); ^^Liou et al. (1990)
45
site, but not to AP-1 (Angel et ah, 1987), MLTF (Carthew et ah, 1985) or E4F2
(Hai et ah, 1988b) binding sites (Hai et ah, 1989). Clones ATF-1 to ATF- 6
were sequenced and were found to share significant amino acid similarity
within their basic domain and leucine zipper (see Figure 1.7.1.3) but shared
little homology outside these regions (Hai et ah, 1989). ATF-1 however,
was found to share extensive homology with CREB even outside the
conserved basic domain and leucine zipper regions (-75% amino acid
similarity; Hai et ah, 1989). Protein sequence analysis of tryptic fragments
of ATF-43 together with immunological analysis have since demonstrated
that ATF-1 corresponds to ATF-43 (Hurst et ah, 1991).
The basic domain/ leucine zipper (bZIP) motif was originally
described in the liver specific transcription factor C/EBP (Johnson et ah,
1987; Landschulz et ah, 1988). The leucine zipper is an amphipathic
a-helix, consisting of a heptad repeat of leucine residues that are aligned
along one face of the helix (Landschulz et ah, 1988) and the interaction
between two leucine zippers brings about the dimérisation of the two
proteins (Kouzarides and Ziff, 1988). The basic domain consists of two
clusters of the basic amino acids lysine and arginine, separated by an
alanine spacer and a conserved asparagine residue (Landschulz et ah,
1988). The leucine zipper also functions to position the two basic domains
of the dimerised proteins within the major groove of DNA (Gentz et ah,
1989) where the positively charged (basic) amino acids make direct contact
with the negatively charged DNA (Vinson et ah, 1989).
Independent investigations have identified binding sites containing
the ATF consensus sequence in several other viral and cellular promoters
and several proteins that bind to such sites have been cloned and found to
belong to the ATF family (see Figure 1.7.1.3). As a result there is duplicity
in the identification of some proteins, with different notations being used
in each case. For these proteins, I will adopt the notation described by Hai
46
et al. (1989); for reference the alternative names for these proteins are
listed in Table 1.7.1.3.
1.7.2 E4F.
Raychaudhuri et al. (1987) characterised an E4 binding activity that
is strongly induced upon adenovirus infection. Whole cell extracts from
mock and adenovirus-infected cells were fractionated using heparin-
agarose chromatography and the fractions were compared for their ability
to bind to regions of the E4 promoter in a gel-mobility shift assay. One
fraction contained a factor (E4F) that binds to E4 sequences from positions
+32 to -91 and which is strongly induced following adenovirus infection.
Using footprint analysis, E4F was shown to interact with the
E4 promoter (Raychaudhuri et al., 1987) within the -39/-56 site observed in
other studies (Lee and Green, 1987; Watanabe et al., 1988; see Figure 1.6.2).
E4F was also suggested to bind to the homologous site present at positions
-150/-172 (see Figure 1.6.2), since binding to the +32/-90 probe can be
inhibited by addition of a competitor containing E4 sequences from -91 to
-224 (Raychaudhuri et ah, 1987). In addition, E4F was shown to be an
E4-specific factor, since competitors containing sequences from the ElB, E2,
E3, MLP and hsp70 promoters do not affect binding of E4F to the
E4 promoter (Raychaudhuri et ah, 1987).
Raychaudhuri et al. (1989) purified E4F by fractionation and DNA
affinity chromatography and showed it to be a 50 kDa protein when
analysed by SDS gel electrophoresis and silver staining. Consistent with
previous suggestions that E4F may be involved in the activation of the
E4 promoter, the fractions from the DNA-affinity column that contained
E4F were shown to be able to stimulate transcription from the
E4 promoter, but not the MLP promoter, in vitro.
47
1.7.3 Other proteins that bind to the E4 promoter.
In addition to ATF and E4F, several other proteins have been found
to interact with the E4 promoter. EivF displays the same DNA binding
specificity as E4F and can specifically activate transcription of the
E4 promoter in vitro (Cortes et al, 1988). C/EBP (CCAAT-box/ enhancer
binding protein) can bind to ATF binding sites from various promoters
and activates transcription of a promoter containing the E4 -39/-56 and
-150/-172 sites in vivo (Bakker and Parker, 1991). E4BP4 displays a similar
DNA binding specificity to that of E4F and analysis of its activity in vivo
demonstrates that it functions as a transcriptional repressor (Cowell et al,
1992). E4TF1 specifically interacts with the E4 promoter in the region
between positions -132 and -152 (see Figure 1.6.2). The binding site for
E4TF1 overlaps with, but is distinct from the ATF binding site in this
region, since the binding specificity of E4TF1 is different from that of ATF
(Watanabe et al., 1988). Finally, E4F2 binds to the region between positions
-149 and -155 (see Figure 1.6.2). In the crude nuclear extract, this DNA
binding activity cannot be detected because its footprint is masked by the
ATF footprints in adjacent regions (Hai et ah, 1988b).
1.8 Trans-activation of the E4 promoter by ElA.
The above studies demonstrate that several different cellular
transcription factors interact with a limited number of binding sites in the
E4 promoter. Although the functional relationship between many of these
factors is unknown, the activities of two of these factors, E4F and a
member of the ATF family (ATF-2), has been analysed in detail in respect
to their individual roles in trans-activation of the E4 promoter by ElA.
Two models for the mechanism of ElA trans-activation of the
E4 promoter are postulated from the study of E4F and ATF-2 function. The
48
first model involves the post translational modification of a transcription
factor by an ElA associated or an ElA dependent protein kinase, that
increases its activity and hence stimulates transcription from the
E4 promoter. The second model involves a direct or indirect interaction
between ElA and a promoter bound transcription factor, that serves to
localise E lA at the E4 promoter allowing the activating domain to
stimulate transcription.
1.8.1 E4F mediated fraws-activation of the E4 promoter by ElA.
E4F is characterised as an E4 binding activity that is strongly induced
upon adenovirus infection (Raychaudhuri et ah, 1987). This suggests that
E4F may be involved in frans-activation of the E4 promoter by ElA. This
argument is strengthened by the observation that the adenovirus induced
increase in E4F DNA binding is ElA dependent, since extracts from cells
infected with the mutant ElA virus dl312 do not display induced E4F
activity (Raychaudhuri et ah, 1987). Analysis of the kinetics of induction of
E4F during infection with the wild type virus reveals a rapid increase
within 3 h of infection, and then maintenance of this level through 4.5 h
of infection. Thus, the level of E4F present in infected cells closely
corresponds to transcription of the E4 gene (Raychaudhuri et ah, 1987).
E4F DNA binding activity was also found to be regulated during
differentiation of mouse F9 teratocarcinoma cells (Raychaudhuri et ah,
1989), consistent with the presence and regulation of a cellular activity
similar to the viral ElA (Impériale et ah, 1984). Extracts of F9 cells were
found to contain substantial levels of E4F activity, independent of a viral
infection, whereas E4F activity can not be detected in extracts of
differentiated F9 cells. Adenovirus infection of the differentiated F9 cells.
49
which thus provides viral ElA function, was found to induce the
reappearance of E4F activity (Raychaudhuri et ah, 1989).
Raychaudhuri et al. (1989) showed that the DNA binding activity of
a preparation of purified E4F is inactivated upon its incubation with
agarose-bound calf intestinal phosphatase, suggesting that the regulation
of E4F activity may involve a phosphorylation event. This inactivation of
E4F DNA binding activity by phosphatase treatment is not an irreversible
event since an extract prepared from adenovirus-infected cells and
fractionated by heparin agarose chromatography to remove active E4F is
capable of reactivating E4F DNA binding activity upon incubation with
the phosphatase-treated sample. In contrast, a similarly fractionated extract
prepared from dl312- (E1A-) infected cells can not restore E4F activity.
Consistent with a phosphorylation dependent activation of E4F DNA
binding activity, a 50 kDa polypeptide that co-purifies with E4F was
labelled with [y-32p] aTP during this reactivation of E4F activity
(Raychaudhuri et ah, 1989).
Rooney et al. (1990) further characterised the DNA binding
properties of E4F and demonstrated that it can be distinguished from ATF
by their different DNA binding properties. Méthylation interference
analysis of the binding of purified E4F and ATF to the E4 -39/-56 site
clearly demonstrates that the DNA binding specificities of E4F and ATF
overlap but differ. A summary of the results of this analysis is presented in
Figure 1.8.1. Méthylation of the G residues at positions -46 and -49 on the
non-coding strand of the E4 promoter DNA and at position -48 on the
coding strand inhibit binding of both ATF and E4F to the E4 -39/-56 site.
However, méthylation of the G residue at -53 on the coding strand
prevents binding of E4F, but does not affect binding of ATF to this site (see
Figure 1.8.1). In addition, the effects of several point mutations in the
E4 -39/-56 site on the binding of purified E4F and ATF were examined in a
50
E4F
ATF
* *
AAAATGACGTAACGGTTTTTTACTGCATTGCCAA
* *
AAAATGACGTAACGGTTTTTTACTGCATTGCCAA
Figure 1.8.1: Méthylation interference analysis of E4F and ATF binding to the -39/-56 site of the E4 promoter. Methylated G-residues in the ATF/E4F binding site that inhibit binding of E4F (top) and ATF (bottom) are in bold type and are indicated by an asterix. From Rooney et al, 1990.
51
gel mobility shift assay. A summary of the results of this analysis is
presented in Table 1.8.1. A point mutation (PM4) strongly inhibits binding
of E4F, but has no effect on the binding of ATF (see Table 1.8.1). In
addition, purified E4F is unable to bind to ATF sites from the E2A and
E3 promoters (see Table 1.8.1). Hence the consensus binding site for E4F is
different to that of ATF, and the E4F binding sites from the E4 promoter
are considered to overlap with the ATF sites (Rooney et ah, 1990; see Table
1.8 .1).
Rooney et al. (1990) postulated that if ATF was responsible for
mediating this process, any ATF binding site might be able to confer ElA
inducibility. However, if E4F was the target of frans-activation by ElA,
then only E4F binding sites (which are a limited subset of ATF sites) would
be able to confer ElA inducibility. Utilising the differences in the sequence
recognition of ATF and E4F described above, Rooney et al. (1990)
attempted to define the nature of the factor involved in mediating ElA
frflns-activation of the E4 promoter by examining the relative roles of
these two activities on the activation of a heterologous promoter by ElA
in vivo. A simple promoter was constructed that contains only ATF or
E4F binding sites upstream of a TATA element and its response to ElA
was examined by co-transfection in a transient expression assay. The
TATTTAT sequence from the simian virus 40 (SV40) promoter was
chosen for this synthetic promoter (Rooney et al., 1990) since previous
experiments have shown that this element is not responsive to E lA
(Simon et ah, 1988).
Rooney et al. found that a promoter containing a duplicated
ATF/E4F binding site from the E4 promoter was ElA inducible when
assayed in Vero cells. However, promoters containing duplicated ATF
binding sites from either the E2A or E3 promoters were not ElA inducible.
That these test promoters were functional was indicated by the finding
52
a
ATF BINDING SITES SEQUENCE ATF E4F
ADENOVIRUS E3 (-55) T G A C G A A A G ++ +ADENOVIRUS E2 (-80) T G A C G T A G T ++ + -ADENOVIRUS E4 (-147) T G A C G A T T T ++ + -
(-230) T G A C G T A G G +++ -(-260) T G A C G T G G C + 4-4- -
ADENOVIRUS E4 (-50) T G A C G T A A C + + + +++(-163) T G A C G T A A C + + + +++
WT T G A C G T A A C + + + +++PMl T a A C G T A A C - -
PM2 T G A t G T A A C - +PM4 T G A C G T A t C + + + -PM5 T G A a G T A A C n /d n /dPM6 T G A C t T A A C n /d n /d
PM5/6 T G A a t T A A C n /d n /d
CONSENSUS ATF T G A C G TCONSENSUS E4F T G A C G T A A C
Table 1.8.1: Binding specificities of ATF and E4F for binding sites from various adenovirus early promoters. From Rooney et al. (1990). Bracketed numbers refer to the position of the binding site with respect to the start site of transcription.
that each was inducible by cAMP (Rooney et ah, 1990). These results were
interpreted to demonstrate that a promoter that can bind E4F (and ATF as
well) is ElA inducible, whereas a promoter that can only bind ATF is not.
To further test this hypothesis, Rooney et al. constructed another series of
promoters identical to those described above, but which contained
duplicated ATF/E4F binding sites harbouring the point mutations
described in Table 1.8.1. As described above, the promoter containing the
wild type ATF/E4F binding site was found to be ElA inducible. Promoters
containing the PMl and PM2 mutations, which inhibit both ATF and E4F
binding, were both found to be unresponsive to ElA. In addition a
promoter containing the PM4 mutation, which does not affect binding of
ATF but which eliminates binding of E4F, was also found to be
unresponsive to ElA. This promoter was shown to be functional by its
ability to respond to cAMP (Rooney et ah, 1990). This result was
interpreted as confirmation of the hypothesis described above, that a
promoter that can bind E4F is ElA inducible, whereas a promoter that can
only bind ATF is not. Hence in the context of this heterologous promoter,
binding of E4F but not ATF is essential for activation by ElA.
1 .8 . 2 ATF-2 mediates tmMS-activation by ElA.
Several of the adenovirus early promoters contain binding sites for
the ATF family of cellular transcription factors (Lee et al., 1987a),
suggesting the involvement of an ATF protein in the ElA transcriptional
response. Liu and Green (1990) examined the activities of two members of
the ATF family for their ability to recruit ElA to the promoter (as
postulated in the model described above; Martin et al., 1990) and
demonstrated that a specific member of the ATF family, ATF-2, can bind to
54
the E4 promoter and function as an ElA inducible transcription factor (Liu
and Green, 1990).
A chimeric protein consisting of ATF-2 fused to the Gal4 DNA
binding domain is able to activate transcription in vivo in a manner that
is dependent upon Gal4 binding sites in the test promoter and on the
presence of ElA (Liu and Green, 1990), as determined by co-transfection in
a transient expression assay. Mutations that destroy either the promoter
targeting domain, the activating domain, or the zinc finger motif of ElA
(Lillie and Green, 1989; Martin et ah, 1990) also prevent activation by ElA
in this assay (Liu and Green, 1990). Hence these results are consistent with
the model hypothesised by Martin et al. (1990) which requires that ElA
contains an activating domain and is able to interact with a promoter
bound protein.
As further evidence for an interaction between ElA and ATF-2, Liu
and Green demonstrated that the roles of these two proteins can be
switched. A Gal4-E1A fusion protein containing amino acids 150-223 of
ElA (and therefore lacking the activating domain) cannot stimulate
transcription from a test promoter containing Gal4 binding sites in the
same assay (Liu and Green, 1990; Martin et ah, 1990). However, this fusion
protein is able to stimulate transcription when co-transfected with an
ATF-2-VP16 fusion protein (Liu and Green, 1990). In this experiment, ElA
behaves as the promoter bound factor and ATF-2-VP16 the frans-activator.
Taken together, these results suggest an interaction between ATF-2
and the promoter targeting domain of ElA. The activating domain of ElA
is therefore localised at a promoter containing ATF binding sites, where it
is able to activate transcription. This interaction is specific to ATF-2 since
another member of the ATF family, ATF-1, does not mediate trans-
activation by E lA in this assay (Liu and Green, 1990). Another ATF
55
protein, CREE, also mediates trans-activation by ElA in this assay,
although less efficiently than ATF-2 (Flint and Jones, 1991).
Mutational analysis of ATF-2 has demonstrated that deletion of the
N-terminal 33 amino acids of this protein destroys its ability to mediate
trans-activation by ElA in the assay described above (Liu and Green, 1990).
Interestingly, this N-terminal region contains a potential zinc finger motif
that shares extensive homology with the C2 H2 zinc fingers found in
TFIIIA (Miller et al, 1985), Spl (Kadonaga et al, 1987), YYl (Shi et al, 1991)
and a variety of other regulatory proteins found in higher and lower
eukaryotes. These structures are characterised by spatially conserved pairs
of cysteine and histidine residues that can function to sequester a single
zinc ion (Miller et al, 1985). Further mutational analysis has demonstrated
that the integrity of this zinc finger motif is essential for mediating trans-
activation by ElA, since a non-conserved substitution of cysteine residues
known to be essential for zinc binding in the homologous structures of
other proteins also destroys the ability of ATF-2 to mediate trans-
activation by ElA (Flint and Jones, 1991; Zu et al, 1991). In addition the
N-terminal 112 amino acids of ATF-2, when fused to the DNA binding
domain of Gal4, were found to be sufficient to mediate frans-activation by
ElA, although further deletion of ATF-2 to leave the N-terminal 83 amino
acids also destroys this function (Flint and Jones, 1991). Hence it is possible
that a second structure in the 80-120 amino acid region is required,
together with the zinc finger motif, for ATF-2 to mediate trans-activation
by ElA.
56
1.9 Aims of this thesis.
Because multiple cellular transcription factors are able to interact
with the E4 promoter, it is difficult to assess which of these factors are
responsible for mediating frans-activation by ElA. An additional
complication is that one particular element in the E4 promoter that is
known to play an important role in this process has been shown to
interact with several distinct transcription factors which may individually
be able to co-operate with ElA to activate the E4 promoter.
The transcription factors E4F and ATF-2 have specific roles in
mediating trans-activation by ElA (Liu and Green, 1990; Rooney et ah,
1990), but their relative roles in activating the E4 promoter have not yet
been adequately addressed. Firstly, the ability of E4F and ATF proteins to
independently mediate trans-activation has only been examined in the
context of a heterologous promoter (Rooney et ah, 1990) and not in the
context of the whole E4 promoter. Secondly, the DNA binding specificity
of ATF-2 has not been examined in detail and hence cannot be compared
to that of E4F.
In order to properly assess the role of E4F in mediating trans
activation of the E4 promoter by ElA, I have introduced point mutations
into the ATF/E4F binding sites in the E4 promoter and monitored their
effects on E4 promoter activity in a transient expression assay in HeLa and
Vero cells. In addition, I have also examined the effects of these point
mutations on the binding of ATF-2 in vitro. Finally, I have explored the
possibility that recombinant ATF-2 could be utilised in an in vi tro
transcription assay to analyse its role in mediating trans-activation of the
E4 promoter by ElA.
57
Chapter 2: Analysis of the requirements for E4 promoter activity in vivo.
A recent study by Rooney et al (1990) demonstrated that a synthetic
promoter consisting of the SV40 TATA box and the two ATF/E4F binding
sites from the E4 promoter is inducible by ElA. However, when these sites
include a point mutation (PM4) which excludes binding of E4F but has no
effect on the binding of ATF, the promoter is no longer ElA inducible.
Rooney et a l therefore concluded that E4F binding to the E4 promoter was
essential for trans-activation by ElA, but that ATF binding was not
necessary.
Other studies do not appear to be consistent with this possibility. A
32 bp element from the vasointestinal peptide promoter (the VIP CRE) can
substitute for E4 promoter sequences upstream of the TATA box (Lee et al,
1989). The VIP CRE is responsible for the cyclic AMP inducibility of the
VIP promoter (Fink et al, 1988; Tsukada et al, 1987) but is not inducible by
ElA in its natural context (Lee e ta l , 1989). However a hybrid promoter
consisting of the VIP CRE fused to E4 promoter sequences including the
TATA box and CAP site (VIPE4CAT) has been shown to be ElA inducible
(Lee et al, 1989; see Figure 2.1.0). The VIP CRE consists of two ATF binding
sites, neither of which resembles the E4F binding site consensus sequence.
The activity of the VIPE4CAT construct therefore appears to be
inconsistent with the observations made by Rooney et al
The heterologous promoter used by Rooney et a l was designed to
isolate the ATF/E4F binding sites it contained as the only known
upstream promoter element (Rooney et al, 1990). However, the results of
Lee et a l (1989) suggest the presence of another element in the
E4 promoter (downstream of the TATA box) that can modify the activity
of ATF binding sites. Together with the observed inconsistency in these
results, this suggests that the conclusion of Rooney et a l that E4F binding
58
to the E4 promoter is essential for its trans-activation by E lA may be
incorrect. By analysing the effect of binding sites characterised for their
ability to bind ATF and E4F on the activity of the E4 promoter in vivo, I
will be able to elucidate the requirements for activation of the E4 promoter
by ElA.
59
2.0 Methods and Materials.
2 .0 . 1 General strategy of construction of new recombinants.
All new recombinant plasmids were constructed according to
standard protocols (Maniatis et al, 1982). Typically, 10 pg of vector was
prepared for ligation to insert DNA by 5-fold over-digestion with the
appropriate restriction enzyme(s) for 1-2 h {e.g. 50 units of enzyme were
used to digest 10 pg DNA in a 50 pi volume) in the reaction buffer
specified and/or provided by the manufacturer.
DN A was purified from the restriction reaction using
phenol/ chloroform extraction and glass milk (BiolOl/Stratech). Small
fragments (<100 bp) excised during the restriction digestion of vector DNA
are removed during this procedure due to the inability of glass milk to
bind DNA fragments smaller than 100 bp. DNA longer than 100 bp was
separated from vector DNA on a low melting point (LMP) agarose gel and
the vector fragment was purified using glass milk (see Chapter 2.0.2.3).
Insert DNA was prepared by digestion with the appropriate
restriction enzyme(s) and subsequent gel purification of the desired
fragment by one of two methods. If the insert DNA fragment was over
100 bp long, it was separated from contaminating DNA on a low melting
point (LMP) agarose gel and purified using glass milk (see Chapter 2.0.2.3).
If the insert DNA fragment was less than 100 bp long, it was separated
from contaminating DNA fragments on a 2% agarose gel and purified by
electroelution (see Chapter 2.0.2.3). Synthetic oligonucleotides were
prepared for ligation to vector DNA by their purification from a
denaturing acrylamide gel by passive elution (see Chapter 2.0.2.3).
Complimentary oligonucleotides were annealed together in 50 mM KCl by
heating to 65°C for 5 min and allowing to cool slow ly to room
temperature.
60
The prepared DNA fragments were checked by agarose gel
electrophoresis prior to their ligation. Typical ligations of vector and insert
DNA would contain 100 ng of vector, either 1 pi or 5 pi of the insert DNA
preparation and 1 pi (10 units) of T4 DNA ligase, in 10 pi of reaction buffer
specified and/or provided by the manufacturer. The products of the
ligation were transformed into competent bacteria (see Chapter 2.0.4)
which were subsequently plated onto L-agar (see Chapter 2.0.4) containing
the appropriate antibiotic for selection.
A number of colonies were picked off the plate using a sterile loop
and grown in 3 ml LB (see Chapter 2.0.3.1) + antibiotic for 5-6 hours.
"Miniprep' plasmid DNA was prepared from 1.5 ml of this culture (see
Chapter 2.0.3.2) and screened for the presence of the correct recombinant
DNA by digestion with appropriate restriction enzymes and analysis by
agarose gel electrophoresis (see Chapter 2.0.2.1). Alternatively, the products
of a restriction digest that yielded 5' overhangs were labelled with either
[32p]-dATP or P^P]-dCTP using AMV reverse transcriptase (see
Chapter 3.0.3). The labelled DNA bands were then separated on a
denaturing polyacrylamide gel (see Chapter 2.0.2.2) and visualised by
autoradiography.
The remainder of the culture used to make the "miniprep' DNA
that yielded a positive clone was used to innoculate 500 ml LB +
ampicillin. This culture was grown overnight and used to prepare a larger
stock of plasmid DNA ('maxiprep' DNA; see Chapter 2.0.3.1) that is
sufficiently pure to be used in a number of applications, including
transfection into human cells, DNA sequencing, in vitro transcription,
etc...
61
2 .0 .2 Electrophoretic analysis of nucleic acids.
2 .0 .2 .1 Agarose gel electrophoresis of nucleic acids.
Horizontal agarose gels were prepared by m elting agarose
(molecular biology grade) in Ix TAE buffer (40 mM Tris-acetate; 1 mM
EDTA pH 8.0) containing 0.5 pg/m l EtBr. Typically, 2% agarose gels were
used to separate DNA fragments less than 500 bp long, whereas 1 % agarose
gels were used to separate larger DNA fragments. The molten gel was
allowed to cool to <65°C before casting in a perspex tray and setting at
room temperature. The DNA sample was applied to the gel in a loading
buffer containing 40% (w /v) sucrose; 0.25% orange G dye and run at 100 V
for 30 min in Ix TAE buffer containing 0.5 pg EtBr. DNA bands were
visualised on a U.V. transilluminator and the gel photographed with a
Polaroid camera using polaroid '667' film.
2.0.2.2 Denaturing polyacrylamide gel electrophoresis of nucleic acids.
Denaturing polyacrylamide gels (10% acrylam ide at 20:1
acrylamide:bisacrylamide; 50% urea; in Ix TBE (90 mM Tris-borate; 2 mM
EDTA pH 8.0) were cross linked by the addition of l/lOO^k vol. 10%
ammonium persulphate and 1/2000*^ vol. of TEMED and poured into a
vertical gel assembly (Protean, BioRad) and allowed to set at room
temperature. The DNA sample was mixed with an equal volume of
loading buffer (90% deionised formalin; Ix TBE; 0.25% bromophenol blue;
0.25% xylene cyanol) and denatured by boiling for 2 min and quenching on
ice. The sample was then loaded on the gel and run at 45 mA for
approximately 2 h in Ix TBE. The gel was then wrapped in 'cling film' and
exposed to X-ray film at -70°C overnight.
62
2.0.2.3 Gel purification of nucleic acids.
DNA fragments larger than 100 bp were purified by separation on a
low melting point (LMP) agarose gel (prepared as for standard agarose gels,
except using low melting point agarose set at 4°C) and excision of the
desired band. The gel slice was melted by the addition of 1 ml saturated
Nal per gram of gel slice and heating at 65°C for 5 min. The DNA fragment
was then purified from the molten agarose using glass milk
(Biol01/Stratech), according to the manufacturers instructions.
DNA fragments smaller than 100 bp were purified by electroelution
from a standard 2% agarose gel (see above). DNA bands were visualised on
a U.V. transilluminator and a well cut in the gel behind the DNA band to
be purified. The well was filled with Ix TAE (minus EtBr) and the current
reversed such that the DNA band was eluted into the well. The current
was applied to the gel in 15-20 second pulses at full power (approximately
40 mA) with the buffer in the well being removed and saved after each
pulse of current. After several pulses, the DNA bands were again
visualised on a U.V. transilluminator to check that all of the required
band had been removed from the gel. The eluted DNA fragment was then
purified with phenol/chloroform and precipitated by the addition of
1 /lOth vol. NaOAc and 2.5 vols. EtOH.
Oligonucleotides were purified prior to their use for cloning by
separation and passive elution from a 15% denaturing polyacrylamide gel
(see above). DNA bands were visualised by U.V. shadowing against a U.V.
fluorescent TLC plate. The desired band was excised from the gel and was
'mashed' with a pipette tip in a 1.5 ml centrifuge tube. This was filled with
elution buffer (0.1% SDS; 0.5 M NH4 OAC; 10 mM MgOAc) and placed on a
rotating wheel for 16 h. The gel was pelleted by brief centrifugation and the
supernatant removed to a clean tube. DNA was precipitated by the
63
addition of 1/10^^ vol. NaOAc and 2.5 vols. EtOH and the DNA pellet was
resuspended in water.
2.0.3 Preparation of plasmid DNA.
2.0.3.1 'Maxiprep' DNA.
500 ml LB (1% w /v bacto-tryptone; 0.5% w /v yeast extract; 1% NaCl)
+ 50 |ig /m l ampicillin was inoculated with 1/1000*^ vol. of a saturated
bacterial culture harbouring the relevant plasmid, and the cells were
grown for 16 h at 37°C with shaking. Cells were harvested by
centrifugation at 3000 rpm for 15 min and cell lysates prepared by alkaline
lysis (Birnboim and Doly, 1979). Briefly, cells were resuspended in 100 ml
solution 1 (50 mM glucose; 25 mM Tris-HCl; 10 mM EDTA; pH 8.0); room
temperature for 5 min; 200 ml solution 2 (0.2 M NaOH; 0.1% SDS) added;
ice for 10 min; 150 ml solution 3 (3 M KO Ac; pH 5.0) added; ice for 30 min.
Cell debris was pelleted by centrifugation at 3000 rpm for 15 min and the
supernatant was strained through sterile gauze into a fresh bottle. DNA
was precipitated by the addition of 0 .6 vols, of isopropanol, incubation at
room temperature for 30 min and centrifugation at 3000 rpm for 15 min.
The supernatant was discarded and the DNA pellet resuspended in 7.5 ml
TE (10 mM Tris HCl; 0.1 mM EDTA; pH 8.0) and 2.5 ml of 10 M NH 4 OAC.
Following incubation on ice for 20 min, the precipitate formed was
pelleted by centrifugation at 3000 rpm for 10 min and the supernatant
transferred to a fresh tube. DNA was precipitated by the addition of
2.5 vols, of EtOH, incubation on ice for 30 min and centrifugation at
3000 rpm for 15 min. The supernatant was discarded and the DNA pellet
resuspended in 500 pi water. RNA was removed by the addition of
RNase A (boiled for 15 min to inactivate DNase) to 50 p g /m l and
incubation at 37°C for 15 min. The solution was adjusted to 1.5 M NaCl
64
and DNA was precipitated by the addition of 0.3 vols, of 30% PEG; 1.5 M
NaCl, incubation on ice for 30 min and centrifugation at 14000 rpm for
10 min. The DNA pellet was resuspended in 400 pi of a Ix proteinase K
buffer (10 mM Tris-HCl; 5 mM EDTA; 0.5% SDS; pH 7.8) and incubated at
55°C for 30 min. Proteinase K was removed by extraction with
phenol/chloroform and the DNA precipitated by the addition of 2.5 vols,
of EtOH, incubation on ice for 30 min and centrifugation at 3000 rpm for
15 min. The final DNA pellet was resuspended in 200 pi water and the
DNA concentration estimated by measuring the O.D. at 260 nm (O.D. of
1.0 = 50 pg/ml).
2.0.3.2 'Miniprep' DNA.
3 ml LB + 50 pg/m l ampicillin was inoculated with a single bacterial
colony and the cells were grown for 16 h at 37°C with shaking. 1.5 ml of
culture was transferred to a 1.5 ml centrifuge tube and cells were harvested
by centrifugation at 14000 rpm for 5 min. Cell lysates were prepared by
alkaline lysis (Birnboim and Doly, 1979). Briefly, cells were resuspended in
100 pi solution 1 (see Chapter 2.0.3.1); room temperature for 5 min; 200 pi
solution 2 (see Chapter 2.0.3.1) added; ice for 10 min; 150 pi solution 3 (3 M
NaOAc; pH 4.5) added; ice for 30 min. Cell debris was pelleted by
centrifugation at 14000 rpm for 10 min and the supernatant was pipetted
into a fresh tube. Proteins were removed by extraction with an equal
volume of phenol/chloroform and DNA was precipitated by the addition
of 2.5 vols, of EtOH, incubation at -20°C for 30 min and centrifugation at
14000 rpm for 10 min. The supernatant was discarded and the DNA pellet
washed in 1 ml 70% EtOH, air dried and resuspended in 20 pi TE (10 mM
Tris-HCl; 0.1 mM EDTA; pH 8.0). RNA was removed during subsequent
digestion with restriction enzymes by the addition of RNase A (boiled for
15 min to inactivate DNase) to 50 pg/m l to the reaction buffer.
65
2.0.4 Preparation of competent bacteria and transformation with
recombinant plasmid DNA.
Competent Eschericia coli strain MC1061 were prepared by the
standard CaCl2 procedure. Briefly, 100 ml of culture was inoculated with
1:1000* vol. of an overnight culture into LB. The cells were grown at 37°C
with shaking until the O.D. at 600nm = 0.5. The cells were cooled on ice for
15 min and harvested by centrifugation at 3000 rpm. The cell pellet was
resuspended in 20 ml ice cold 50 mM CaCl2 (pH 6.0) and the cells incubated
on ice for 30 min. The cells were again harvested by centrifugation at
3000 rpm and the cell pellet was resuspended in 50 mM CaCl2 ; 15%
glycerol. The cells were incubated on ice for between 15 min and 1 h, and
aliquots of 50 pi were snap-frozen using liquid nitrogen and stored at
-80°C.
For a typical transformation, an aliquot of competent cells were
thawed by warming in the palm of the hand and immediately placed on
ice. Approximately 10 ng of DNA (1 pi of a ligation) was added to the cells
and incubated on ice for 30 min. The cells were then heat shocked by
immersion in a water bath at 42°C for 90 sec. and returned to ice. 300 pi LB
was then added and the cells incubated at 37°C for 1 h to allow the cells to
express the antibiotic resistance gene present on the transformed plasmid.
Various amount of this mixture were then plated onto LB agar plates (1%
w /v bacto-tryptone; 0.5% w /v yeast extract; 1% NaCl; 1.5% bacto-agar)
containing 50 pg/m l ampicillin and the transformed cells grown at 37°C
overnight.
66
2.0.5 Transient transfection assay.
2.0.5.1 CaCl2 /phosphate transfection of plasmid DNA into mammalian
cells.
Mammalian cells (HeLa and Vero) were grown in E4 medium at
37°C and maintained by regular re-plating at 1/10* density from confluent
90 mm tissue culture plates. The cells were separated from the tissue
culture plates by washing with a 3:1 mix of versine/0.25% trypsin
following aspiration of the medium. Plasmid DNA containing the test
promoter linked to the CAT reporter gene was transfected into
mammalian cells by CaCl2 /phosphate precipitation. Typically, 5 pg of the
plasmid containing the test promoter and 15 pg of a carrier DNA (such as
pSP64); or 5 pg of the plasmid containing the test promoter, 5 pg of a
plasmid expressing the ElA gene (pH3G; see below) and 10 pg of a carrier
DNA, were transfected into cells split 1:8 from a confluent plate into
35 mm tissue culture plates 8 h previously. pH3G contains the Hindlll-G
fragment of adenovirus type 5 (nucleotide positions 1-2804) encoding the
entire ElA region cloned into pBR322 (Green et a l , 1983).
The precipitation of the DNA involved mixing 500 pi of a 240 mM
CaCl2 solution containing the DNA, with 500 pi of 40 mM Hepes;
275 mM NaCl; 1.4 mM Na2HP0 4 ; 1.4 mM NaH2P0 4 ; pH 7.1; dropwise and
with continual gentle mixing. The mixture was incubated at room
temperature for 30 min and 500 pi was added to the cells dropwise. The
cells were grown at 37°C for 16 h, after which the medium was changed
and the cells were grown for a further 24 h at 37°C. The cells were then
washed in 5 ml PBS and harvested by scraping off the plate in 1 ml PBS
into a 1.5 ml centrifuge tube. The cells were then pelleted by brief
centrifugation and resuspended in 50 pi 250 mM Tris-HCl; ImM EDTA;
67
pH 7.8. Crude cell extracts were prepared by three cycles of freeze/thaw and
the cell debris was pelleted by centrifuging at 14000 rpm for 2 min.
2.0.5.2 Assay of chloramphenicol acetyl transferase activity.
Crude cell extracts prepared from transfected cells was analysed for
CAT activity. 27 pi of the crude cell extract was incubated with 0.125 pCi
[ C] chloramphenicol in an 80 pi reaction containing 1.25 mM acetyl CoA;
215 mM Tris HCl; pH 7.5, for 1 h at 37°C. The products of the reaction were
extracted by vortexing with 2 0 0 pi ethyl acetate and brief centrifugation to
separate the aqueous and organic phases. 170 pi of the ethyl acetate phase,
which contains all forms of chloramphenicol, was carefully removed and
subsequently concentrated to approximately 1 0 pi by vacuum evaporation.
This was spotted onto a silica gel TLC ready foil (Schleicher and Schuell)
and allowed to dry before being subjected to ascending chromatography
with a 95:5 mixture of chloroformimethanol. Finally, the plate was
allowed to dry before being examined by autoradiography.
The activity of a promoter was deduced by excising the
[ C] chloramphenicol and acetylated-[^^C] chloramphenicol spots from the
TLC plate and counting the amount of radioactivity present in each using
a scintillation counter. The activity of the promoter is expressed as
% conversion of the chloramphenicol to its acetylated forms and is
calculated using the following formula;
% conversion = counts per minute acetylated chloramphenicol x 1 0 0
counts per minute total chloramphenicol
In each assay, this was calculated from the average of at least two
separate transfection experiments measuring the activity of multiple
preparations of the plasmid containing the test promoter.
68
2.1 Results.
2.1.0 An ATF binding site that does not bind E4F can mediate
trans-activation of the E4 promoter by ElA.
To address whether an ATF binding site that does not bind E4F can
confer ElA inducibility upon the E4 promoter, I constructed a hybrid
promoter (E2CAT) that consists of a duplicated ATF binding site from the
adenovirus E2A promoter placed upstream of the E4 TATA box and CAP
region. E2CAT therefore resembles the hybrid promoter VIPE4CAT, with
the ATF binding sites from the E2A promoter replacing the VIP CRE (see
Figure 2.1.0 A). Whereas it is unknown as to whether E4F binds to the VIP
CRE, the ATF binding site in the E2A promoter has previously been
shown not to bind E4F (Rooney et al., 1990; see Table 1.8.1).
ElA inducibility was tested in a transient expression assay in HeLa
cells, in which the test promoter is linked to the chloramphenicol acetyl
transferase gene {cat) as a reporter. The ability of ElA to induce expression
of cat was assayed in the presence and absence of a co-transfected ElA gene.
Representative autoradiograms of the assay are shown in Figure 2.1.0 B.
These illustrate the ElA inducibility, but not the absolute activity of the
test promoter, since different amounts of cell extract were used for each
individual assay. The amount of ^^C-chloramphenicol present in each of
the spots from the TLC was measured in a scintillation counter (data not
shown) and used to calculate the activity of the test promoter in the
presence and absence of ElA. This data has been adjusted to compensate
for the amount of extract used in each assay and is shown graphically in
Figure 2.1.0 C. The activity of the E2CAT promoter is expressed as a
percentage of the induced activity of a positive control (E4CAT) which
corresponds to that of the wild type E4 promoter.
69
Adenovirus E4 Promoter
EivF E4TF1 EivF
TATA
-325 -240 -174 -50 -38 +32
E4A38 ItataI 1 CAT
E4CAT TATA CAT
VIPE4
E2CAT
>TATA
TATA
Figure 2.1.0 A: Structure of the E4A38, E4CAT, VIPE4 and E2CAT test promoters. E4CAT (Lee et al , 1989) and VIPE4 (Lee et al , 1989) have been described elsewhere. E4CAT contains E4 promoter sequences (positions -240 to 4-32) fused to the chloramphenicol acetyltransferase (CAT) gene at position 4-32 relative to the E4 transcription start site. E4A38 contains E4 promoter sequences from -38 to 4-32 fused to the CAT gene. VIPE4 contains sequences between positions -94 and -61 of the vasoactive intestinal peptide (VIP) promoter (the cyclic AMP response element [CRED inserted into E4A38 at position -38 of the E4 promoter sequences.
E4A38Sac contains a S a d linker inserted at position -38 of the E4 promoter sequences in E4A38. E2CAT was obtained by cloning an oligonucleotide containing a duplicated ATE binding site from the adenovirus E2A promoter (cAACTACGTCATCTCCAACTACGTCATCT- CCgagct; E2A sequences are in uppercase, linker sequences are in lowercase, and the ATF binding sites are underlined) into the S a d site of E4A38SacCAT.
70
y / / /# # # # • • t •
E1A: - + - 4- - -k - 4-
Figure 2.1.0 B: Activation of synthetic promoters by ElA; Representative CAT assays. The test promoter is indicated above each lane and the presence (+) or absence (-) of ElA is indicated at the bottom of the figure. A 5 pg sample of the test plasmid was introduced into HeLa cells (grown on 60 mm plates at -30% confluence) with 10 pg of pSP64 (minus ElA) or 5 pg pSP64 plus 5 pg pH3G (plus ElA).
100 n
<
+E 1A
E4C A T E4A38 E2C A T
Promoter
Figure 2.1.0 C: Activation of synthetic promoters by ElA; Quantitation of CAT assays. Relative promoter activity (an arbitary scale reflecting percent converted ^^C-chloramphenicol per milligram protein) is charted for each promoter in the presence and absence of ElA, with the activity of E4CAT in the presence of ElA representing 100% activity.
72
As reported previously, ElA efficiently activates the E4 promoter in
this assay (31 fold activation by ElA). In addition, ElA also activates the
E2CAT promoter (9 fold activation), although to a lesser extent than
E4CAT. The corresponding promoter that is lacking the ATF binding sites
and contains only the E4 TATA box and CAP region (E4A38) is essentially
inactive in this assay.
Although E2CAT is induced by ElA, the activity of this promoter is
much lower than that of the E4 promoter (less than 10%). The difference
in the activities of these two promoters may be due to the presence of
binding sites for other cellular factors (such as SPl) and the additional ATF
binding sites that are present in E4CAT but not E2CAT (see Figure 2.1.0 A).
However, despite the differences in the overall activity of these two
promoters, these results demonstrate that an ATF binding site that does
not bind E4F can mediate frans-activation of the E4 promoter by ElA.
2 .1 .1 Spl binding sites are unable to mediate efficient trans-activation of
the E4 promoter by ElA.
A variety of elements other than ATF or E4F binding sites have
been shown to mediate trans-activation by ElA. Weintraub and Dean
(1992) demonstrated that both ATF and Spl binding sites could
individually confer ElA inducibility upon a heterologous promoter
containing the SV40 TATA box. In contrast, other studies have suggested
that Spl binding sites present in the E4 promoter are not necessary for its
activation by ElA, since deletion of these sites only reduces the overall
activity of the promoter and not its inducibility by ElA (Gilardi and
Perricaudet, 1984). However, this conclusion does not take into account
the possibility that more than one E4 promoter element is able to mediate
73
E4CAT ATF mm TATA CAT
E2CAT %BTATA I 1 C A T ^ ^
SplCAT TATA CAT
Figure 2.1.1 A: Structure of the SplCAT test promoter. E2CAT and E4CAT are shown for comparison and are described in Eigure 2.1.0 A. SplCAT was obtained by cloning an oligonucleotide containing a duplicated Spl binding site (AAATGGGGCGGGGCTTAGAAATGGGGCG- GGGCTTAgagct; linker sequences are in lowercase, the Spl binding sites are underlined) into the Sad site of E4A38SacCAT.
74
SplCAT E4CAT
# eElA: - + - +
Figure 2.1.1 B: Activation of the synthetic promoter SplCAT by ElA; Representative CAT assays. The test promoter is indicated above each lane and the presence (+) or absence (-) of ElA is indicated at the bottom of the figure. A 5 pg sample of the test plasmid was introduced into HeLa cells (grown on 60 mm plates at -30% confluence) with 10 pg of pSP64 (minus ElA) or 5 pg pSP64 plus 5 pg pH3G (plus ElA).
100 n-E lA
+E 1A8 0 -
> 6 0 -u<
40 -
2 0 -
E4C A T S P IC A T
Promoter
Figure 2 .1 .1 C: Activation of the synthetic promoter SplCAT by ElA; Quantitation of CAT assays. Relative promoter activity (an arbitary scale reflecting percent converted chloramphenicol per milligram protein) is charted for each promoter in the presence and absence of ElA, with the activity of E4CAT in the presence of ElA representing 100% activity.
76
frans-activation by ElA, and that SPl binding sites can indeed mediate this
response in the absence of other upstream promoter elements.
To address whether Spl binding sites are interchangeable with ATE
binding sites in the context of the E4 promoter, I constructed a synthetic
promoter that contains a duplicated binding site for Spl upstream of the
E4 TATA box and CAP region (SplCAT). SplCAT therefore corresponds to
E2CAT, with the Spl sites replacing the ATE binding sites from the E2A
promoter (see Figure 2.1.1 A).
SplCAT activity was assayed in HeLa cells in the presence and
absence of ElA. Representative autoradiograms of the assay are shown in
Figure 2.1.1 B and the data is shown graphically in Figure 2.1.1 C. Like
E2CAT, the induced activity of SPICAT is much lower than that of E4CAT
(less than 15% as active). However in contrast to E2CAT, SplCAT is only
induced 3 fold by ElA, compared to the 9 fold induction of E2CAT. This
result demonstrates that activation of E2CAT by ElA is specific to the ATE
binding sites from the E2A promoter and is not a general effect of placing a
promoter element upstream of the E4 TATA box. This would suggest that
the E4 promoter does have specific requirements for activation by ElA and
that these are distinguishable from the requirements of other E lA
inducible promoters.
2 .1 .2 E4F binding is not required for activation of the intact E4 promoter by
ElA.
The results described above suggest that E4F binding is not required
for activation of the intact E4 promoter by ElA. However, the activity of
E2CAT is far less than the E4 promoter (approximately 5% compared to
E4CAT) suggesting that this synthetic promoter may not be representative
of the activity of the intact E4 promoter. To properly assess the
77
requirements for activation of the E4 promoter whilst avoiding the
limitations of the experiments described above, I constructed a series of
promoters that correspond to the normal E4 promoter, but which contain
point mutations in their ATF/E4F binding sites. The mutations used in
this study have already been characterised for their effects on the binding
of E4F and ATE in an in vitro DNA binding assay (Rooney et ah, 1990) and
are summarised in Table 1.8.1.
pWE4WT corresponds to the normal E4 promoter and was
constructed by the ligation of synthetic oligonucleotides into a vector
containing the cat reporter gene, to recreate E4 promoter sequences
between +32 and -174. This region of the E4 promoter has previously been
shown to give activity equivalent to that of the entire E4 promoter (Gilardi
and Perricaudet, 1984; Lee and Green, 1987). Base substitutions in the E4
sequence were made to create unique restriction sites in the
oligonucleotides in order to facilitate the construction of the mutant
promoters and were chosen to minimise the sequence differences between
these promoters and the normal E4 promoter (see Figure 2.1.2 D).
WE4PM1, WE4PM2 and WE4PM4 correspond to pWE4WT but contain the
corresponding point mutation in each of the two ATF/E4F binding sites
present at positions -50 and -163 in the E4 promoter (see Figure 2.1.2 A).
The promoters were assayed in HeLa cells in the presence and
absence of FIA. Representative autoradiograms of the assay are shown in
Figure 2.1.2 B and the data is shown graphically in Figure 2.1.2 C. WF4WT
is comparable with F4CAT in overall activity and FIA inducibility.
Therefore minor changes to the F4 promoter sequence arising from the
introduction of novel restriction sites (Figure 2.1.2 D) have no effect on
F4 promoter activity. Consistent with previous studies, upstream
sequences to position -174 give activity that is comparable with that of
F4CAT. Mutation PM2 (WF4PM2) causes a substantial reduction in
78
E4CAT mm TATA CAT
IE4WT
IE4PM
CAT
—^ It a t a I 1 C A T ^ ^
WE4WT
WE4PM
TATA CAT^ (0 I^ IC A T ^
Figure 2.1.2 A: Structure of the IE4 and WE4 series of promoters. E4CAT is shown for comparison, and is described in Eigure 2.1.0 A. Restriction site overhangs were chosen so as to minimize the number of base changes introduced by the construction procedure (see Eigure 2.1.2 D). pE4Sac2CAT was constructed by inserting an oligonucleotide containing E4 promoter sequences from -33 to +32 and a Sad site at position -33, between the Hindlll and Ndel sites of RSVCAT. pIE4WT was obtained by inserting an oligonucleotide containing E4 promoter sequences from -38 to -60 and from -154 to -174 (separated by a Sall/Sacll linker) into the Sad site of pE4Sac2CAT. pIE4WT therefore contains the two ATF/E4E binding sites from the E4 promoter positioned upstream of the TATA box. pIE4PMl, 2 and 4 were constructed in the same manner with oligonucleotides harboring the corresponding point mutations. pWE4WT was obtained by ligating an oligonucleotide containing E4 promoter sequences from positions -67 to -148 to pIE4WT that had been digested with Sail and Sadi. pWE4PMl, 2 and 4 were constructed in the same manner but using pIE4PMl, 2 and 4 digested with Sail and Sadi as the source DNA.
79
y y y y• • • • # # # # # #
EMA: - + - 4 - - - h - - h - - f
Figure 2.1.2 B: Effects of point mutations on E4 promoter activity; Representative CAT assays. The test promoter is indicated above each lane and the presence (+) or absence (-) of ElA is indicated at the bottom of the figure. A 5 pg sample of the test plasmid was introduced into HeLa cells (grown on 60 mm plates at -30% confluence) with 10 pg of pSP64 (minus ElA) or 5 pg pSP64 plus 5 pg pH3G (plus ElA).
u<
120-
100-
8 0 -
6 0 -
4 0 -
20-
-E lA
+ E1A
IE4PM 1 IE4PM 2 IE4PM 4 IE4W T W E4PM 1 W E 4PM 2 W E 4P M 4 E 4C A T
Promoter
Figure 2.1.2 C: Effects of point mutations on E4 promoter activity; Quantitation of CAT assays. Relative promoter activity (an arbitary scale reflecting percent converted [ C] chloramphenicol per milligram protein) is charted for each promoter in the presence and absence of ElA, with the activity of E4CAT in the presence of ElA representing 100% activity.
81
-174 ATF/E4F *** ATF -136pE4CAT GGAAGTGACGTAACGTGGGA a a a CGGAAGTGACGATTTG
pWE4WT GGAAGTGACGTAACGTGGGA cca CGGAAGTGACGATTTG
SacII
-72 ATF/E4F ** TATA -22TTTTTTGT g GACTTTAACCGTTACGTCATTTTTTAG t C CTATATATACTCG
TTTTTTGT C GACTTTAACCGTTACGTCATTTTTTAG C t CTATATATACTCG
Sail
F igure 2.1.2 D: Sequence differences between pE4CAT and pWE4WT. Base changes introduced into the E4 sequence during the construction of pWE4WT are indicated by an asterix and lowercase letters, and the unique restriction sites created are underlined. ATF and ATF/E4F binding sites are in bold type and positions with respect to the start site of E4 transcription are noted.
82
activity (-10% compared to WE4WT) but remains ElA inducible, although
to a lesser extent (10 fold induction). Mutation PMl (WE4PM1) results in
very low activity (-5% compared to WE4WT) but remains somewhat ElA
inducible (-5 fold induction). In contrast the mutation PM4 (WE4PM4) has
no effect on overall promoter activity or ElA inducibility.
The effect of mutations PMl and PM2 demonstrates that the activity
of the promoter is dependant at least in part on the two ATF/E4F binding
sites and that their functioning is critical for normal promoter activity. In
addition, the lack of an effect of mutation PM4 on E4 promoter activity
demonstrates that binding of E4F to these sites is not required for
activation of the intact E4 promoter by ElA.
2.1.3 Upstream promoter elements other than the ATF/E4F binding sites
are not required for activation by ElA.
The reconstructed promoters described above contain other
upstream promoter elements in addition to the two ATF/E4F binding
sites, including an ATF binding site at position -147. To analyse the
requirement of these additional promoter elements, I tested the activity of
another series of promoters (IE4WT, PM l, PM2 and PM4). IE4WT
corresponds to WE4WT but lacks sequences between -67 and -148 and
therefore only contains downstream sequences inclusive of the TATA box
and CAP region, plus the two ATF/E4F binding sites (Figure 2.1.2 A).
The promoters were assayed in HeLa cells in the presence and
absence of ElA. IE4WT is efficiently induced by ElA (Figure 2.1.2 C, 15 fold
induction) but is somewhat reduced in overall activity (-25% of E4CAT).
The effects of mutations PMl, PM2 and PM4 on this series of promoters
mirror the effects of these mutations on the WE4 series of promoters, but
are correspondingly lower in overall activity; PMl and PM2 give rise to
83
both a substantial reduction in promoter activity and ElA inducibility,
whereas PM4 has no effect on either promoter activity or ElA inducibility.
The reduction in the activity of this series of promoters caused by
removing the E4 promoter sequences between -67 and -148 (compare
IE4WT with E4CAT, Figure 2.1.2 C) could be due to the ATF site at -147 or
the SPl site at position -104 (Figure 2.1.0 A). These results demonstrate that
upstream elements other than the ATF/F4F binding sites are not necessary
for activation of the F4 promoter by FIA. However, these elements do
contribute to the overall activity of the promoter by stimulating both the
basal and induced activities.
2.1.4 F4F binding sites are not required for activation of the F4 promoter by
FIA in Vero cells.
As noted previously, a recent study by Rooney et al. concluded that
F4F binding sites are required for activation of the F4 promoter by FIA,
whereas ATF binding sites are insufficient. These conclusions were based
on the results of experiments using an heterologous promoter, consisting
of F4F binding sites placed upstream of the SV40 TATA box, which was
tested for its ability to mediate FIA inducibility in Vero cells. To determine
whether the different cell types might account for the differences between
my results and those of Rooney et ah, I analysed the activity of the
F4 promoter mutants in Vero cells.
The activity of the IF4WT and IF4PM4 promoters (see
Figure 2.1.2 A) was assayed in Vero cells in the presence and absence of
FIA. Representative autoradiograms of the assay are shown in
Figure 2.1.4 A and the data is shown graphically in Figure 2.1.4 B.
Mutation PM4 (IF4PM4) had no effect on either basal or FlA-induced
promoter activity in Vero cells when compared to IF4WT. This result
84
IE4WT IE4PM4
# •
# # * eElA: 4- - +
Figure 2.1.4 A: Effect of cell type on the requirement for E4F for activation of the E4 promoter by ElA; Representative CAT assays. The test promoter is indicated above each lane and the presence (+) or absence (-) of ElA is indicated at the bottom of the figure. A 5 pg sample of the test plasmid was introduced into Vero cells (grown on 60 mm plates at -30% confluence) with 10 pg of pSP64 (minus ElA) or 5 pg pSP64 plus 5 pg pH3G (plus ElA).
Vero cells
100-
80-
•S 60-u <
4 0 -
2 0 -
-E lA
i. 1 +E 1A
IE4W T IE4PM 4
Promoter
Figure 2.1.4 B: Effect of cell type on the requirement for E4F for activation of the E4 promoter by ElA; Quantitation of CAT assays. Relative promoter activity (an arbitary scale reflecting percent converted [^^C] chloramphenicol per milligram protein) is charted for each promoter in the presence and absence of ElA, with the activity of IE4WT in the presence of ElA representing 100% activity.
86
demonstrates that, as is the case in HeLa cells, E4F binding sites are not
required for activation of the E4 promoter by ElA in Vero cells. Therefore
the differences between the results presented here and those of Rooney
et al. are not due to the different cell lines used in these two assays.
87
2.2 Discussion.
Because the E4 promoter is able to interact with multiple cellular
transcription factors, it has been difficult to assess which of these factors
are responsible for mediating trans-a.ctivation of the E4 promoter by ElA.
Both E4F (Rooney et al, 1990) and ATF-2 (Liu and Green, 1990) have been
suggested to play critical roles in this process and I have examined
whether the binding of either of these factors correlates with E4 promoter
activity in HeLa cells. My results can be summarised as follows. The
ATF/E4F binding sites present upstream of the E4 TATA box are critical
for activation of the E4 promoter by ElA. However, the ability of these
sites to bind E4F does not correlate with trans-activation by ElA, whereas
ATF binding sites alone are sufficient to mediate trans-activation.
These results are in contrast to those of Rooney et a l (1990) who
demonstrated that in the context of a heterologous promoter containing
the SV40 TATA box, E4F binding sites are essential but ATF binding sites
are insufficient for mediating trans-activation by ElA. The overall activity
of these heterologous promoters is extremely low compared to that of the
E4 promoter (RE4WT is less than 4% as active in the presence of ElA;
Jones and Lee, 1991), which together with the contradiction between the
results presented here and those of Rooney et a l , demonstrates the
limitations of analysing the effects of a promoter element upon the
activity of a synthetic promoter. More significantly, a previous report has
demonstrated that ATF binding sites can confer quite distinct properties
when placed in different promoters (Lee et a l , 1989). Therefore the
rationale for using a heterologous promoter to investigate the specific role
of ATF/E4F binding sites in trans-activation of the E4 promoter by ElA
(Rooney et al, 1990) is highly questionable.
88
Despite their limitations, the results of Rooney et al. (1990)
demonstrate that E4F does play a role in this process under some
circumstances, and indeed there is significant circumstantial evidence
suggesting that E4F is involved in mediating frans-activation of the
E4 promoter by E lA (see Chapter 1; Raychaudhuri et ah, 1989;
Raychaudhuri et ah, 1987). Therefore the obvious interpretation of these
results is that ElA can target more than one cellular transcription factor to
activate the E4 promoter. E4F and ATF may be equally able to mediate
frflns-activation of the E4 promoter by ElA, but either factor may be
sufficient in the absence of the other.
There are several reasons for the possible differences in the
activities of the heterologous promoters examined by Rooney et ah and
the E4 promoter. Firstly, it is possible that ATF binding sites can only
confer ElA inducibility in a relevant promoter context, whereas E4F
binding sites do not share the same promoter dependent constraints. For
example, the presence of another E4 promoter element involved in
mediating frans-activation of the E4 promoter by ElA is suggested by the
results of Lee et ah (1989). The cyclic AMP response element (CRE) of the
vaso-intestinal polypeptide (VIP) promoter does not respond to ElA in its
natural context, but when placed upstream of the E4 promoter this
element mediates frans-activation by ElA (Lee et ah, 1989). Hence it is
possible that some other element within the E4 promoter affects the
activity of ATF binding sites such that they can mediate activation by ElA.
Alternatively, functional differences between the TATA boxes of these
promoters may affect the activity of ATF binding sites. The nature of
functional differences between different TATA boxes is not yet
understood, but is likely to reflect the DNA binding activity of general
factors other than TBP and to involve sequences surrounding the defined
TATA box. At least two different TFIID complexes have been described
89
(Timmers and Sharp, 1991) that differ in their complement of TBP
associated factors (TAFs) and it is possible that these factors may contribute
to the sequence recognition of the TATA region. Indeed, other factors
within the TFIID complex contact DNA (Purnell and Gilmour, 1993)
although the nature or specificity of these interactions has not yet been
defined. In addition, TAFs have been shown to act as adaptor proteins in
the activation of transcription by some classes of activator proteins (Tanese
et a l , 1991). Hence a requirement for a specific TFIID complex at a TATA
box would result in a particular complement of TAFs and hence specific
adaptor properties at that promoter. Different adaptor requirements for
ATF and E4F mediated trans-activation by ElA may therefore be
manifested as requirements for a particular TATA box.
Secondly, I cannot rule out the possibility that differences in the
experimental assays used to determine the activity of test promoters in
this study and by Rooney et al. may account for these conflicting results.
One such difference that I have not addressed experimentally is that
unlike the Vero cells used in this study, the assay performed by Rooney
et al. used Vero cells that had been grown under conditions of serum
starvation. Therefore it is possible that dependence on E4F binding for
trans-activation by ElA is linked to the cellular growth state.
It has been proposed that transcriptional activation of the
E4 promoter occurs through an ElA dependent increase in the DNA
binding activity of E4F (Raychaudhuri et al , 1987). This is thought to be
due to a change in the phosphorylation status of E4F that is mediated by an
ElA dependent cellular kinase (Raychaudhuri et al, 1989). Similarly, ElA
trans-activation of the polymerase Ill-transcribed VA RNA genes in vivo
occurs through an ElA dependent phosphorylation of TFIIIC that is
accentuated when cells are grown under low serum conditions (Hoeffler
et al., 1988b). This ElA dependent phosphorylation results in an increase
90
in the association rate of TFIIIC with the VA promoter and produces a
more salt resistant TFIIIC/promoter complex. In addition, these
physiological changes in the DNA binding activity of TFIIIC correlate with
the stimulation of transcription from the VA promoter by the
phosphorylated form of the protein. If TFIIIC and E4F are phosphorylated
by the same cellular kinase, these observations suggest that the activity of
E4F could be regulated by cellular growth factors and might therefore be
influenced by the proliferative state of infected cells.
91
Chapter 3: Determination of the DNA binding specificity of an
N-terminally truncated ATF-2 protein in vitro.
Previous studies have suggested that one member of the ATF
family, ATF-2, mediates activation of the E4 promoter by ElA (Flint and
Jones, 1991; Liu and Green, 1990). This effect is specific to ATF-2 since
another member of the ATF family, ATF-1, was found to be unable to
mediate an ElA-response in the same assay (Flint and Jones, 1991; Liu and
Green, 1990). The results described above are consistent with this
observation, since I have shown that ATF binding sites are required for
trans-activation of the E4 promoter by ElA. However, the binding affinity
of ATF-1 and ATF-2 for the sites characterised in vivo has not previously
been examined. By determining the specificity of binding of these two
members of the ATF family for the mutated ATF/E4F binding sites
described in the above experiments, I will be able to further establish the
nature of the factor(s) required for activation of the E4 promoter by ElA.
92
3.0 Methods and Materials.
3.0.1 In vitro transcription and translation of ATF proteins.
ATF RNA was synthesised in vitro using the T7 transcription
system. A plasmid consisting of the relevant ATF cDNA cloned
downstream of a T7 promoter was linearised by cutting with a restriction
enzyme 3' to the translation stop signal. The cut plasmid was then
extracted with phenol/ chloroform and ethanol precipitated. The DNA was
resuspended in water that had been autoclaved, treated with 0 .2 % diethyl
pyrocarbonate (DEPC) and then re-autoclaved. All further manipulation of
the DNA and RNA were performed using fresh DEPC treated water,
double autoclaved pipette tips and tubes and whilst wearing clean gloves,
in order to minimise contamination of the RNA preparation with
RNases.
4 |ig of the linearised plasmid DNA was added to a transcription
reaction containing; 40 mM Tris-HCl pH 7.5; 6 mM MgCl2; 2 mM
spermidine; 100 |ig /m l BSA (RNase free; Boehringer Mannheim); 10 mM
DTT; 100 |iM GTP; 500 [iM each of ATP, CTP and UTP; 500 |iM CAP
analogue (m^GpppG; Boehringer Mannheim); 100 u RNasin (Boehringer
Mannheim); 10 u T7 RNA polymerase (Boehringer Mannheim) in a total
volume of 80 pi. The reaction was incubated for 1 h at 40°C, after which
the plasmid DNA was removed by the addition of 40 u of DNase I (RNase
free; Boehringer Mannheim) and incubation at 37°C for 10 min. 15 pg
tRNA and 1.5 vols, of 0.7 M NH 4 OAC were added, followed by extraction
with phenol/chloroform and ethanol precipitation of the RNA. The RNA
pellet was resuspended in 10 pi DEPC water and 1 pi was checked on a 2%
agarose gel.
Protein was synthesised from this RNA preparation in vitro using a
lysate from rabbit reticulocytes (Boehringer Mannheim). To test the RNA
93
preparation as a template for translation, 1 pi was incubated with 24 pi of
the lysate and 30 pCi P^SJ-methionine for 1 h at 30°C. The translation
reaction was then run on a 10% SDS/PAGE gel (see Chapter 3.0.2) and the
synthesised ATF protein was visualised by autoradiography. For a large
scale translation, 12 pi RNA was incubated with 380 pi of lysate and
120 pCi p5S]-methionine for 1 h at 30°C. Aliquots of 20 pi the translation
reaction were quick frozen in liquid nitrogen and stored at -80°C.
3.0.2 SDS/polyacrylamide gel electrophoresis of proteins.
SDS/polyacrylamide gels were prepared with a resolving gel of 10%
acrylamide (at 37.5:1 acrylamideibisacrylamide; in a buffer containing
375 mM Tris-HCl pH 8 .8 ; 0.1% SDS) and a stacking gel of 5% acrylamide (at
37.5:1 acrylamide:bisacrylamide; in a buffer containing 125 mM Tris-HCl
pH 6.8; 0.1% SDS) and were cross linked by the addition of 1 / 80th vol. 10%
ammonium persulphate; l/lOOO i vol. of TEMED, poured into a vertical
gel assembly (Protean, BioRad) and allowed to set at room temperature.
The protein sample was mixed with an equal volume of loading buffer
(125 mM Tris-HCl pH 6.8; 20% Glycerol; 4% SDS; 10% p-mercaptoethanol;
0.005% bromophenol blue; 0.25% xylene cyanol) and denatured by boiling
for 2 min. The sample was then loaded on the gel and run at 45 mA for
approximately 2 h in Ix running buffer (25 mM Tris; 190 mM Glycine;
0.1% SDS).
Proteins were visualised by staining the gel with coomassie blue.
The gel was soaked in staining solution (0.2% coomassie blue; 50%
methanol; 7.5% glacial acetic acid) for 10-20 min and then destained in 10%
methanol; 7.5% glacial acetic acid until protein bands were clearly
distinguished from the background staining.
94
3.0.3 Analysis of the DNA binding activity of proteins using a gel
retardation assay.
A DNA fragment containing an ATF binding site was excised from
a plasmid using relevant restriction enzymes and isolated from a 2 %
agarose gel by electroelution (see Chapter 2.0.2.3). 50 ng of the purified
DNA fragment was labelled with 20 pCi [a^^F]-dATP or [a^^P]-dCTP using
10 u AMV reverse transcriptase (Boehringer Mannheim) for 30 min at
37°C in 20 pi of a reaction buffer specified by the manufacturer. The
reaction mixture was made up to 50 pi total volume and extracted with
phenol/chloroform before use.
1 ng of the DNA probe was incubated with the protein sample for
15 min in 15 pi of a buffer containing 10 mM Tris-HCl pH 7.5; 60 mM
NaCl; 1 mM DTT; 1 mM EDTA; 5% glycerol, either in the presence or
absence of a competitor oligonucleotide. 2 pi of loading buffer (50%
glycerol; 0.25% bromophenol blue; 0.25% xylene cyanol) was then added
and the reaction was loaded on a neutral acrylamide gel (5% acrylamide at
50:1 acrylamide:bisacrylamide; 6.7 mM Tris-HCl pH 8.3; 1 mM EDTA;
3.3 mM NaOAc). Gels were run in Ix retard buffer (6.7 mM Tris-HCl
pH 8.3; 1 mM EDTA; 3.3 mM NaOAc) at 45 mA for approximately 2 h, with
continuous circulation of the electrophoresis buffer.
95
3.1 Results.
3.1.0 ATF-1 and ATF-2 bind specifically to ATF binding sites present in
various adenovirus early promoters.
To determine whether the requirement for an ATF binding site for
activation of the E4 promoter by ElA is consistent with a requirement for
ATF-2, I analysed the specificity of ATF-2 for ATF binding sites and
compared this to the binding specificity of another ATF protein, ATF-1. I
was not able to efficiently produce full length ATF-2 protein using a
T7 polymerase/rabbit reticulocyte lysate in vitro system (data not shown)
so an N-terminally truncated form of the protein (DL2; Benbrook and
Jones, 1990) which was efficiently produced in this system was instead
used for these assays. This truncated protein lacks amino acids 1-232, but
still contains the leucine zipper and basic domains (located between amino
acids 350-410) that are thought to be sufficient for sequence specific DNA
binding activity.
Studies of the binding specificity of ATF-1 and ATF-2 were
performed using an in vitro gel-mobility shift assay. ATF proteins were
produced by the in vitro transcription and translation of their cDNA and
bound to a 32p_labelled DNA probe containing an ATF binding site. When
run on a neutral polyacrylamide gel, the resulting D N A /protein
complexes are retarded compared to the unbound probe, due to their
slower mobility on the gel (Figure 3.1.0). No binding is observed when
ATF-1 (data not shown) and ATF-2 (Figure 3.1.0 B) mRNA is omitted from
the translation reaction, demonstrating that the protein/DNA complexes
formed are dependent upon ATF proteins.
Binding specificity was analysed by using a competition assay in
which unlabelled oligonucleotides were added to the binding reaction to
96
B
COMPETITOR
COMPLEXlATF1
UNBOUNDDNA
ex CM ^ ^ ^ LU UJ UJ UJ lü
nLU
i nf I
1 2 3 4 5 6 7 8
C - ^A T F 2
1 2
s 3 sa CM ^ ^ CO «
LU UJ LU UJ UJ UJ
3 4 5 6 7 8 9
Figure 3.1.0: (A) Binding of ATF-1 to adenovirus early promoters. Gel mobility shift assays were performed with ATF-1 synthesized in vitro in rabbit reticulocyte lysate and incubated with a ^^P-labelled DNA probe containing an ATF binding site. Unbound DNA and ATF-1/DNA complexes are indicated on the left. Assays were performed in the presence of a 200 fold excess of competitor DNA as indicated above the lanes. Competitor DNAs are described in the text.
(B) Binding of ATF-2 to adenovirus early promoters. ATF-2 protein was synthesized in vitro, and gel mobility shift assays were performed as described for ATF-1. Unbound DNA (U) and ATF-2/DNA complex (C) are indicated at the left. Lane 1, incubation with ATF-2 in the absence of competitor DNA; Lane 2, incubation of 32p-labelled DNA probe with rabbit reticulocyte lysate in the absence of ATF-2.
compete with the labelled probe for binding of ATF proteins. ATF-1
(Figure 3.1.0 A) and ATF-2 (Figure 3.1.0 B) bind to several competitor
oligonucleotides containing ATF binding sites found in the adenovirus
E2A (E2WT) and E3 (E3WT) promoters or a consensus ATF binding site
(E4WT). This is demonstrated by the absence of the shifted probe in these
lanes of the gels. However neither protein binds to the corresponding
competitor oligonucleotides carrying a mutation or deletion of the ATF
binding site (E2LS, E3M, E4M1 and E4M2), as is demonstrated by the
presence of the shifted probe. Thus ATF-1 and ATF-2 specifically bind to
all of the ATF binding sites tested. This further demonstrates the
difference in the binding specificity between E4F and specific members of
the ATF family, since E4F does not bind to ATF binding sites found in the
adenovirus E2A and E3 promoters.
3.1.1 The effect of point mutations on the binding specificity of ATF-1 and
ATF-2.
By characterising the DNA binding specificity of ATF-1 and ATF-2
using the point mutations that affect E4 promoter activity, I will be able to
determine whether the binding specificity of either protein is consistent
with their being involved in activation of the E4 promoter by ElA.
Binding specificity was analysed by adding increasing amounts of
unlabelled competitor oligonucleotides containing certain point
mutations, to a binding assay using a 32p_labelled DNA probe containing
an ATE binding site. Similar to the assay described above, an unlabelled
competitor that binds the protein efficiently will cause a reduction in the
amount of shifted probe on the gel with an increasing amount of
competitor. An unlabelled competitor containing a mutated site that
cannot bind the protein will have no effect on the shifted probe. The
98
ATF1
MOLAR EXCESS 60 , 240 , 960 X
#= liiwt
U
WT PM1 PM2 PM4 PM5 PM6
COMPETITOR
Figure 3.1.1 A: Effects of point mutations on ATF-1 binding. Gel mobility shift assays were performed as described in the legend to Figure 3.1.0 A. Unbound DNA (U) and ATF-1/DNA complexes (C) are indicated at the left. Assays were performed in the presence of increasing amounts of unlabelled oligonucleotide competitors containing either a wild type (WT) ATF binding site from the F4 promoter or the point mutations described in Table 1.8.1 as indicated at the bottom of the figure. The molar excess of competitor oligonucleotide is indicated at the top of the figure.
MOLAR EXCESS : 50150500.
ATF2
COMPETITOR: W T P M 4
0 20 60180
* - # - BC:. BBUD
PM 1 P M 2 P M 4 P M 5 P M 6 P M 5 /6
Figure 3.1.1 B : Effects of point mutations on ATF-2 binding. Gel mobility shift assays were performed as described in the legend to Figure 3.1.0 B. Unbound DNA (U) and ATF-2/DNA complexes (C) are indicated at the left. Assays were performed in the presence of increasing amounts of unlabelled oligonucleotide competitors containing either a wild type (WT) ATF binding site from the E4 promoter or the point mutations described in Table 1.8.1 as indicated at the bottom of the figure. The molar excess of competitor oligonucleotide is indicated at the top of the figure.
molar excess of competitor oligonucleotide containing the wild type ATF
binding site required to compete for binding of ATF proteins to the probe
DNA was deduced from the results of several preliminary experiments
(data not shown). A range of amounts of competitor DNA up to this pre
determined molar excess of the wild type competitor were then compared
for binding to competitor DNAs containing mutated the ATF binding
sites.
In this assay ATF-1 binds the competitor oligonucleotide containing
mutation PM4 as efficiently as that containing the wild type (WT) ATF
binding site (see Figure 3.1.1 A). Oligonucleotides containing mutations
PMl and PM2 compete very weakly and oligonucleotides containing
mutations PM5 or PM6 do not compete at all in the range tested (see
Figure 3.1.1 A). Thus PMl, PM2, PM5 and PM6 are strong down mutations
for ATF-1 binding whereas mutation PM4 has no effect. The lack of effect
of the PM4 mutation on ATF-1 binding further establishes the different
binding specificity of ATF-1 and E4F.
The effect of point mutations on ATF-2 binding are shown in
Figure 3.1.1 B. As is the case for ATF-1 binding, an oligonucleotide
containing mutation PM4 competes for ATF-2 binding as efficiently as the
wild type (WT) oligonucleotide. However, oligonucleotides containing
mutations PMl or PM2 also compete efficiently for ATF-2 binding. My
supervisor. Dr. Kevin Lee, also demonstrated that oligonucleotides
containing mutations PM5 or PM6 compete less efficiently for ATF-2
binding and that the effect of these mutations is confirmed by the inability
of an oligonucleotide containing the double mutation (PM5/6) to compete
for ATF-2 binding (Jones and Lee, 1991; see Figure 3.1.1 C). The lack of an
effect of PMl and PM2 on the binding of ATF-2 suggests that the binding
specificity of ATF-2 does not correlate with activation of the E4 promoter
by ElA in vivo.
101
3.1.2 The effect of point mutations on the stability of ATF-2 binding.
The above experiments demonstrate the affinity of a protein for a
particular binding site, but they give no indication of the stability of
binding. This can be assessed by binding the protein to a ^^P-labelled DNA
probe containing the relevant site in the absence of competitors. An
unlabelled competitor oligonucleotide containing a site known to bind the
protein efficiently is then added for varying lengths of time before loading
the binding reaction on the gel. The stability of binding can be expressed as
the time it takes the competitor to reduce the amount of shifted probe by
50% (tl/2 ).
To further assess the effect of these point mutations on ATF-2
binding my supervisor. Dr. Kevin Lee, examined the stability of ATF-2
binding to ^^P-labelled DNA probes containing mutations PMl, PM2 and
PM4 (Jones and Lee, 1991; see Figure 3.1.2). Before the addition of
competitor oligonucleotides (time=0) ATF-2 binds equally well to probes
containing the mutations PMl, PM2 and PM4, thus confirming the lack of
effect of mutations PMl and PM2 on ATF-2 binding. Upon addition of
competitor oligonucleotides, ATF-2 dissociates from each probe at a
similar rate, demonstrating that mutations PMl and PM2 do not affect
ATF-2 binding stability.
In addition to further demonstrating that mutations PMl and PM2
have no effect upon the binding affinity of ATF-2, this result also
establishes that these mutations have no effect upon the stability of
binding of ATF-2. Therefore the effects of these mutations on the DNA
binding specificity of ATF-2 do not correlate with their effects on the
activity of the E4 promoter in vivo.
102
TIME : 0 0 0 10 30120
ATF2
U
J L J L J L
PROBE : PM4 PM1 PM2 PM4
Figure 3.1.2: Stability of ATF-2 DNA binding to point mutated ATF/E4F binding sites. ^^P-labelled oligonucleotide containing mutations PM l, PM2 and PM4 (indicated at the bottom) were used to examine the effects of these mutations on the stability of ATF-2 binding. Gel mobility shift assays for ATF-2 were performed as described in the legend to Figure 3.1.0. At various times before the samples were loaded on the gel (time in minutes at the top of the figure), an excess of specific competitor DNA (PM4 oligonucleotide) was added to the binding reaction. Lane 1, control containing no competitor throughout the incubation period; Lane 2, control containing competitor before formation of stable complexes. Provided by Dr. Kevin Lee; from Jones and Lee (1991).
3.1.3 The effect of point mutations on the binding of ATF-2 to the
E4 promoter in vivo.
Contradictory with previous reports which demonstrate that ATF-2
can mediate frans-activation of the E4 promoter by ElA (Flint and Jones,
1991; Liu and Green, 1990), the results described above suggest that it is
unlikely that ATF-2 homodimers mediate activation of the E4 promoter
by ElA in HeLa cells. However, it is possible that the in vitro DNA binding
assay described above may not reflect the binding activity of ATF-2 to the
mutated E4 promoters in vivo, or alternatively that the DNA binding
specificity of the truncated ATF-2 protein is not the same as that of the
wild type protein.
Previous studies have demonstrated that ATF-2 binds to the
ATF/E4F binding sites in the E4 promoter in vivo (Liu and Green, 1990)
utilising a chimeric protein consisting of the full length ATF-2 protein
(Hai et al, 1989) fused to the acidic activation domain of VP16 (Triezenberg
et al., 1988). Activation of the E4 promoter by the ATF-2/VP16 fusion
protein was demonstrated in a transient expression assay in which the test
promoter (E4CAT) was co-transfected with a plasmid expressing
ATF-2/VP16 (pAct-CR2; Liu and Green, 1990). ATF-2 alone does not
efficiently activate transcription in this assay, and activation by the
ATF-2/VP16 fusion protein is dependent on the presence of ATF binding
sites in the test promoter (Liu and Green, 1990). In this assay, the DNA
binding domain of ATF-2 serves to recruit the acidic activation domain of
VP16 to the E4 promoter in order to activate transcription.
This assay was used to analyse the effects of point mutations PMl
and PM2 upon the DNA binding activity of ATF-2 in vivo, by measuring
activation by the ATF-2/VP16 fusion protein of a test promoter containing
the mutated ATF/E4F binding sites. Initial attempts to observe efficient
104
IE4WTxlO IE4PMlxlO IE4PM2xlO
#
ATF-2/VP16######
Figure 3.1.3 A: Activation of the IE4xlO series of promoters by an ATF-2/VP16 fusion protein; Representative CAT assays. The test promoter is indicated above each lane and the presence (+) or absence (-) of a plasmid expressing the ATF-2/VP16 fusion protein (pAct-CR2) is indicated at the bottom of the figure. A 5 ig sample of the test plasmid was introduced into HeLa cells (grown on 60 mm plates at -30% confluence) with 10 pg of pSP64 (minus ATF-2/VP16) or 5 |ig pSP64 plus 5 pg pAct-CR2 (plus ATF-2/VP16).
-A T F -2 /V P 1 6
Ü + A T F -2 /V P 1 6
IE4W T 1E4PM1 IE4PM 2 xlO xlO xlO
Promoter
Figure 3.1.3 B: Activation of the IE4xlO series of promoters by an ATF-2/VP16 fusion protein; Quantitation of CAT assays. Relative promoter activity (an arbitary scale reflecting percent converted [^^C] chloramphenicol per milligram protein) is charted for each promoter in the presence and absence of the ATF-2/VP 16 fusion protein, with the activity of IF4WTxlO in the presence of ATF-2/VP16 representing 100% activity.
106
activation of both the WE4WT and IE4WT promoters by the ATE-2/VP16
fusion protein were unsuccessful, giving rise to a very low level of
transcriptional activation (data not shown). In order to observe more
efficient activation by the ATF-2/VP16 fusion protein, I constructed a
series of promoters consisting of 5 duplicated pairs of ATE/E4F binding
sites placed upstream of the E4 TATA box and CAP region (IE4WTxlO,
PMlxlO and PM2xlO), supposing that the presence of multiple binding
sites in the test promoter may enhance activation by the ATF-2/VP16
fusion protein. These promoters was constructed by the cloning of
additional oligonucleotides containing the relevant binding sites into the
IE4 series of promoters, such that each construct contains the relevant
point mutation in each of its 10 ATF/E4F binding sites.
The promoters were assayed in HeLa cells in the presence and
absence of a plasmid expressing the ATF-2/VP16 fusion protein described
above. Representative autoradiograms of the assay are shown in
Figure 3.1.3 A and the data is shown graphically in Figure 3.1.3 B.
IF4WTxlO is induced 6.5 fold by ATF-2/VP16 in this assay, demonstrating
that ATF-2 binds to the ATF/F4F binding sites in vivo. Mutation PM2
(IF4PM2xlO) causes a slight reduction in the inducibility by ATF-2/VP16
(~3 fold activation) and was less than 50% as active as IF4WTxlO in the
presence of ATF-2/VP16. Mutation PMl (IF4PMlxlO) also causes a
reduction in the inducibility by ATF-2/VP16 (4.5 fold activation) and in
addition substantially reduces the overall activity in the presence of
ATF-2/VP16 to less than 25% that of IF4WTxlO.
This experiment has only been performed once in duplicate and the
variability was such that in order to confirm the precise level of activation
of each of these promoters by the ATF-2/VP16 fusion protein this
experiment will need to be repeated. However, if there proves to be a
reproducible effect of these point mutations on activation by the
107
ATF-2/VP16 fusion protein as is suggested by the above results, I would
conclude that although ATF-2 binds to PMl and PM2, either the DNA
binding affinity and/or binding stability of ATF-2 to these mutations is
impaired to some extent in vivo. However, the small effect of these
mutations on ATF-2 binding in vivo does not correlate with their more
pronounced effects on activation of the E4 promoter in vivo. Therefore,
these results are further evidence that the DNA binding specificity of
ATF-2 does not correlate with activation of the E4 promoter in vivo.
108
3.2 Discussion
A previous study by Rooney et a l (1990) has analysed the effects of
several point mutations in the ATF/E4F binding sites of the E4 promoter
on the binding of E4F and an ATF binding activity purified from HeLa
cells. Utilising the effects of these point mutations upon the DNA binding
activity of E4F, I have shown that E4F binding is not required for trans-
activation of the E4 promoter by ElA. Liu and Green (1990) have
demonstrated that a specific member of the ATF family, ATF-2, can
mediate trans-activation by ElA and that this protein binds to the
E4 promoter in vivo. However, the precise nature of the DNA binding
specificity of ATF-2 has not previously been assessed. I have analysed the
DNA binding specificity of ATF-2 in vitro to determine whether binding
of ATF-2 to the E4 promoter correlates with its trans-activation by ElA
in vivo. My results can be summarised as follows. The DNA binding
specificity of an N-terminally truncated ATF-2 protein in vitro does not
correlate with trans-activation of the E4 promoter by E lA in vivo.
Although these point mutations may have a small effect on the DNA
binding activity of an ATF-2/VP16 fusion protein in vivo, these results are
nevertheless inconsistent with their more pronounced effects on
activation of the E4 promoter by ElA in vivo.
Previous reports have demonstrated that ATF-2 can function with
ElA to activate the E4 promoter (Liu and Green, 1990) and that ATF-2
mRNA is expressed in HeLa cells (Maekawa et a/., 1989). Therefore the
results described above which demonstrate that the DNA binding
specificity of ATF-2 does not correlate with activation of the E4 promoter
in HeLa cells is unexpected. One explanation for this result is that ATF-2 is
not present as a homodimer in HeLa cells, but only as a heterodimer with
another bZIP protein. ATF-2 is known to form heterodimers with ATF-3
109
(Hai et al., 1989) and c-jun (Benbrook and Jones, 1990) and it is possible that
either of these heterodimers could display a different affinity for the
mutated ATF/E4F binding sites to that of the ATF-2 homodimer.
It is also possible that the DNA binding assays used do not
accurately reflect the DNA binding specificity of ATF-2 in vivo, and
differences in the results from the in vitro and in vivo assays described
suggest that this may indeed be the case. These differences could be
attributed to one or more of several factors. Firstly, the in vitro gel
retardation assay utilised an N-terminally deleted ATF-2 protein which
may not display the same DNA binding properties as the full length
protein. This would suggest that another unknown functional domain,
distinct from the basic domain, can modulate the DNA binding activity of
ATF-2.
Recent evidence has demonstrated that the DNA binding activity of
CREB is influenced by its phosphorylation at a site distinct from the basic
domain (Nichols et ah, 1992). CREB contains a phosphorylation site for
protein kinase A (PKA) that is distant to the basic domain and leucine
zipper region and which is not required for DNA binding. However,
phosphorylation of this site in vivo increases the occupancy by CREB of a
weak, asymmetric binding site present in the tyrosine aminotransferase
(TAT) promoter (Nichols et ah, 1992). Hence although the PKA site is not
directly involved in DNA binding, it can influence the function of the
basic domain and indirectly affect the DNA binding specificity and/or
affinity of CREB for a particular site.
ATF-2 has been shown to contain several sites for cellular kinases
(Sakurai et ah, 1991) and hence the DNA binding activity of this protein
may also be regulated in a similar maimer. If this is the case, then different
phosphorylation patterns of the truncated ATF-2 protein made in vitro
and of the full length ATF-2 protein in vivo could account for differences
110
in the DNA binding specificity of these two proteins. Such differences in
the phosphorylation of the two proteins could be attributed either to
different compliments of kinases in HeLa cells and the rabbit reticulocyte
lysate, or to the lack of one or more phosphorylation sites in the truncated
ATF-2 protein.
Alternatively, the putative zinc finger present in the N-terminal
region may modulate the DNA binding specificity of ATF-2. The C2 H2 zinc
finger of ATF-2 shares homology to the zinc fingers of several other
proteins (see Figure 3.2) such as TFIIIA (Miller et al, 1985), Spl (Kadonaga
et a l , 1987) and YYl (Shi et al, 1991), and the zinc fingers of these proteins
are known to be involved in the sequence-specific recognition of DNA.
Unlike ATF-2, all of these other proteins contain multiple copies of the
C2H2 motif which each contribute to the overall DNA binding specificity
of the protein. Single units of these zinc fingers do not recognise specific
DNA sequences (Parraga et al, 1988) and therefore the single zinc finger of
ATF-2 may be expected not to impart sequence specificity upon the
protein. However it is possible that the zinc finger motif of ATF-2 may
contact DNA in a relatively non-specific manner such as to modulate the
DNA binding specificity conferred by the basic domain.
Secondly, it is possible that the DNA binding specificity of ATF-2
could be altered upon interaction with ElA, such that it cannot bind to the
mutated ATF/E4F binding sites. Interestingly, the hepatitis B virus (HBV)
X gene product (pX) has been reported to have a similar effect on the
binding of both ATF-2 and CREB to the HBV enhancer (Maguire et al,
1991). Neither pX, ATF2 or CREB alone can bind to the HBV enhancer;
however, pX has been shown to form complexes with either ATF-2 or
CREB and to specifically alter their DNA binding specificity such that these
complexes can then interact with the HBV enhancer (Maguire et al, 1991).
I ll
N>
ATF-2: K P F L C T A P 6 C G Q R F T N E D H L A V H K H K H E
S p l2 (1 ) K Q H I C H I Q G C G K V Y G K T S H L R A H L R W H T(2) R p F M C T W S Y C G K R F T R S D E L Q R H K R T H T
YY13 (1) R T I A c P H K G c T K M F R D N S A M R K H L H T H G(3) K P F Q c T F E G c G K R F S L D F N L R T H V R I H T(4) R P Y V c P F D G c N K K F A Q S T N L K S H I L T H A
TFIIIA4 (1) K R Y I c S F A D c G A S Y N K N W K L R A H L C K H T(2) K P F p c K E E G c D K G F T S L H H L T R H S I T H T(4) C V Y V c H F E G c D K A F K K H N Q L K V H Q F T H T(5) L P Y K c P H E G c D K S F S V P S C L K R H E K V H A(9) R P F A c E H A E c G K S F A M K K S L E R H S V V H D
Consensus K F c c K F L R H H
Figure 3.2: Comparison of the zinc finger motif of ATF-2 with several representative C2 H2 zinc fingers. Consensus residues are boxed, and homology to ATF-2 is in bold. Bracketed numbers denote the position of the zinc finger with respect to the other zinc fingers in the protein.
(1985)References: ^Hai et al. (1989); Maekawa et al. (1989); ^Kadonaga et al. (1987); ^Shi et al. (1991); ^Miller et al.
Finally, the in vivo binding assay that I have described is highly
artificial and I cannot discount either the effects of the binding of ATF-2 to
10 sites in such close proximity, or of the VP16 fusion on the binding
specificity of ATF-2. I have shown that PMl and PM2 have a strong
negative effect on binding of ATF-1 (and presumably CREB binding also).
Therefore these mutations may increase the opportunity for the
ATF-2/VP16 fusion protein to occupy these sites in vivo compared to
binding to the wild type site or to a site containing the PM4 mutation.
Hence any deleterious effect of these mutations on the DNA binding
specificity of ATF-2 in vivo may be masked rather than accentuated in the
absence of binding of other factors. Hence that I observe any effect at all of
the PMl and PM2 mutations on ATF-2 binding could be enough of an
indication that ATF-2 binding does in fact correlate with activation of the
E4 promoter in vivo.
To establish the nature of the differences in the DNA binding
specificity of these two proteins, we must first determine whether or not
the DNA binding specificity of full length ATF-2 is different from that of
the truncated protein in vitro. This was originally not possible because full
length ATF-2 could not be expressed efficiently in the in vitro system
described above or by overexpression in bacteria. However, I have since
constructed a His-tagged ATF-2 protein lacking the N-terminal 15 amino
acids that is efficiently overexpressed in E. coli (see Chapter 4.2). The effects
of functional domains of ATF-2 other than the basic domain (such as the
phosphorylation sites or the zinc finger) upon the DNA binding specificity
of the full length protein can hopefully be examined in vitro b y
mutational analysis of this recombinant ATF-2. Obviously it is possible
that this protein will still not reflect the binding specificity of the full
length protein, but since the missing 15 amino acids account for only 3%
of the proteins length, this is an unlikely possibility.
113
Chapter 4: Analysis of E4 promoter activity in vitro.
Because ATF binding sites are able to interact with multiple cellular
transcription factors (Hai et al, 1989) it is difficult to assess the role of an
individual factor in mediating trans-activation of the E4 promoter by ElA
in vivo. An in vitro transcription assay that faithfully reproduces trans
activation of the E4 promoter by ElA could be useful in examining the
role that ATF-2 plays in this process and in further analysing the
mechanisms involved. However, neither the ability of ATF-2 to activate
transcription in v it ro , nor the ability of ElA to t rans -ac t iva te the
E4 promoter in vitro, have been previously demonstrated.
Previous studies have demonstrated that the E4 promoter can be
accurately transcribed in vitro using crude nuclear extracts (Dignam et al,
1983; Lee and Green, 1987). Lee and Green (1987) demonstrated that the
E4 promoter was extremely transcriptionally active in such an assay and
was at least 1 0 -fold more active than the highly efficient adenovirus major
late promoter (MLP). When low amounts of promoter template were used
in this assay, the ratio of E4/MLP transcription was found to be particularly
high (Lee and Green, 1987).
It is now clear that the majority of the ATF binding activity present
in HeLa cells is attributable to ATF-1 and CREB. In vitro DNA binding
assays in which nuclear extracts from HeLa cells are incubated with a
32P-labelled DNA probe containing an ATF binding site give rise to
complexes that correspond to those produced by ATF-1, CREB and their
heterodimer (Hurst e ta l , 1991). DNA affinity purification of ATF binding
proteins from HeLa cell nuclear extracts results in high yields of both
43 kDa and 47 kDa proteins (relative to other proteins) that correspond to
ATF-1 and CREB by several criteria (Hai et al, 1988b; Hurst et al, 1990).
These affinity purified proteins and cloned CREB have been shown to
114
stimulate transcription in vitro (Gonzalez et al, 1991; Hai et al , 1988b) and
cloned CREB and ATF-1 have been shown to activate transcription
in vivo (Flint and Jones, 1991; Gonzalez e t a l , 1991; Lee e t a l , 1990;
R ehfuss e t a l , 1991). Therefore it is likely that ATF-1 and CREB are
responsible for the high level of transcriptional activity of the E4 promoter
in vitro observed by Lee and Green (1987). However, neither of these
proteins appears to be involved in mediating trans-activation by ElA
in vivo (Flint and Jones, 1991; Liu and Green, 1990) and this may explain
why trans-activation of the E4 promoter by ElA has not previously been
demonstrated in vitro.
Studies of trans-activation of the E2A and MLP promoters by ElA
in vitro have demonstrated that the addition of recombinant ElA to a
transcription assay is sufficient to bring about activation (Spangler et al,
1987). In addition, a 49 amino acid synthetic peptide that comprises the 46
amino acids unique to the larger ElA protein, plus 3 adjacent and
conserved amino acids, was found to be capable of stimulating
transcription from the E3 and MLP promoters in vitro (Green et al, 1988;
Loewenstein and Green, 1989). However, the effect of ElA in these assays
is not as pronounced as its effect on the same promoters in vivo (for
example see; Impériale et al, 1985; Lewis and Manley, 1985). Together with
the observation that the E4 promoter is extremely active in vitro (Lee and
Green, 1987) and that this high level of activity is most likely due to CREB
and ATF-1, this suggests that it is unlikely that ElA-dependent activation
of the E4 promoter could be observed in a standard in vitro transcription
assay utilising crude nuclear extracts.
The transcriptional activity of the E4 promoter in vitro can be
reduced significantly by the addition of a specific competitor
oligonucleotide containing an ATF binding site to the nuclear extract prior
to a transcription assay (Lee and Green, 1987), due to sequestering of ATF
115
binding proteins endogenous to the nuclear extract. Transcriptional
activity can then be restored by the subsequent addition of purified ATF
proteins (Hai etal., 1988a). Using this approach, it may be possible to
develop a transcription assay that demonstrates activation of the
E4 promoter by ElA and which is dependent on the addition of
recombinant ATF-2. ATF-2 has been shown to be a relatively poor
activator in vivo (Flint and Jones, 1991; Liu and Green, 1990) and hence
addition of recombinant ATF-2 to an ATF depleted in vitro transcription
assay may be expected to have little or no effect on the activity of the
E4 promoter. Hence I may be able to demonstrate ElA dependent
activation of an ATF depleted transcription reaction by either one of two
strategies. The simplest approach would be the comparison of activation
by recombinant ATF-2 and ElA together, to that of ATF-2 alone.
Alternatively, I could compare the effect of adding recombinant ATF-2 to
an ATF depleted transcription reaction using nuclear extracts from
adenovirus infected HeLa cells to one using nuclear extracts from
uninfected cells.
116
4.0 Methods and Materials.
4.0.1 In vitro transcription of the E4 promoter.
Activity of the E4 promoter was analysed using pE4CAT as a
template for transcription. This consists of the E4 promoter from positions
+240 to -38 fused to the chloramphenicol acetyl transferase (CAT) gene
(Lee et a l , 1989). Activity of the MLP promoter was analysed using pMLP
as a template for transcription. This consists of the Ball-E fragment from
adenovirus type 2 (map units 14.7-21.5) including the major late promoter,
cloned into the BamHI site of pBR322 (Manley et a l , 1980). Nuclear
extracts were prepared as described by Dignam et a l (1983) with slight
modifications and were used to direct transcription from the E4 promoter
as described by Lee and Green (1987). Briefly, 1 pg of the promoter template
was incubated with 26 pi of nuclear extract in 40 pi of a buffer containing
0.5 mM each of ATP, CTP, GTP and UTP; 7.5 mM MgCl2 ; 50 u RNasin
(Boehringer Mannheim); at 30°C for 1 h. The transcription reaction was
treated with proteinase K (at 600 pg/m l) in 260 pi of a buffer containing
0.4% NP40; 100 mM NaCl; 7.75 mM Tris-HCl pH 8 .6 ; 1.15 mM MgCl2 ; at
55°C for 30 min. The mixture was then extracted with an equal volume of
phenol/ chloroform and ethanol precipitated.
Detection of the RNA transcribed from each promoter was by a
previously described method of primer extension, using ^^P-end-labelled
synthetic oligonucleotides as probes to either the CAT or MLP products.
10 pmoles of primer was labelled with 100 pCi [y32p]-ATP using 20 u
polynucleotide kinase in 15 pi of a buffer specified by the manufacturer.
The reaction was made up to 100 pi volume and extracted with
phenol / chloroform prior to use. The RNA pellet from the transcription
reaction was resuspended in 15 pi 0.5% SDS (in DEPC water) and
hybridised to 0.1 pmole of this labelled primer in 30 pi of hybridisation
117
buffer (40 mM PIPES pH 6.4; 1 mM EDTA; 400 mM NaCl; 80% formamide)
overnight at 37°C. The primer/RNA hybrid was then precipitated by the
addition of 400 |il of 60% isopropanol; 400 mM NH^OAc and
centrifugation at 14000 rpm. The RNA pellet was washed with 70%
ethanol and resuspended in 10 pi of DEPC water. The primer was extended
with 2 u AMV reverse transcriptase (Boehringer Mannheim) in 20 pi of a
buffer specified by the manufacturer, for 1 h at 42°C. The products of the
reaction were then resolved on a denaturing (urea) polyacrylamide gel (see
Chapter 2.0.2.2) and visualised using autoradiography.
4.0.2 Expression and purification of recombinant proteins.
Recombinant CREB proteins were overexpressed in E. coli by
cloning the rat CREB cDNA into the pET vector, such that the CREB open
reading frame was fused (in frame) to pET sequences encoding a poly
histidine tail. This fusion protein is expressed under the control of an
inducible T7 promoter and the BL21 host strain (a protease deficient strain
of E. coli) expresses the T7 polymerase. pET/CRla (encoding the H^CRla
fusion protein) was constructed as follows. The rat CREB cDNA was
completely digested with H ind l l l (which cuts the cDNA in the 3'
untranslated region) and then partially digested with Ncol (which cuts the
cDNA at the methionine start codon). The 'sticky' ends of the DNA created
by these restriction enzymes were blunted w ith AMV reverse
transcriptase) and the desired fragment isolated from a 1 % LMP agarose gel
using glass milk. This was then ligated to pET 15b that had been digested
with Xhol and blunted with AMV reverse transcriptase.
To construct a transcriptionally deficient CREB protein, a P v u l l
fragment (within the H gC R la coding sequences) was excised from
pE T /C R la. The resulting construct, pET/CRlAP, encodes the H^CRIAP
fusion protein, which lacks CREB amino acids 58 to 192 and hence all
118
regions of the CREB protein known to be involved in transcriptional
activation.
The pET constructs encoding the recombinant CREB proteins were
transformed into BL21, a protease deficient strain of E. coli. A single colony
containing the vector was used to inoculate 5 ml LB and grown overnight
at 37°C. This culture was used to inoculate 11 NZYCM (85 mM NaCl;
9.8 mM MgCli; 1% NZ amine; 0.5% yeast extract; 0.1% cas-amino acids) and
was grown at 37°C until the culture was in the log phase of growth. This
was determined by measuring the absorbance of the culture at 600 nm
(A5 0 0) until it reached A qo = 0.4. Expression of the recombinant fusion
protein was induced by the addition of IPTG to a final concentration of
0.5 mM and the cells were grown for a further 2 h. The cells were collected
by centrifugation, resuspended in 20 ml ice cold lysis buffer (Ix PBS; 0.5%
Triton XlOO; 1 mM EDTA; 1 mM PMSF; 5 p g /m l trasylol; 5 p g /m l
leupeptin), lysed by sonication on ice and the cell debris removed by
centrifugation at 4°C. 1 ml of a nickel-agarose column (Ni^+-NTA agarose;
Qiagen) was added to the supernatant and incubated on a rotating wheel at
4°C for 30 min. The histidine tail of the fusion protein forms a strong
interaction with the nickel column and the bound protein was washed
free of contaminating bacterial proteins with ice cold lysis buffer. The
fusion protein was eluted from the column by mixing with 1 ml of
250 mM imidazole in Ix PBS for a few minutes. The purified protein was
dialysed against 1000 volumes of 20 mM Hepes pH 8.0; 125 mM KCl; 1 mM
DTT; 15% glycerol overnight and aliquots of 20 pi were quick frozen on
liquid nitrogen and stored at -80°C. The yield and size of the purified
protein was checked by SDS/PAGE (followed by coomassie staining) and
gel retardation analysis.
119
4.1 Results.
4.1.1 Reduction of E4 transcription by the addition of a competitor
oligonucleotide containing an ATF binding site.
Previous reports have dem onstrated that a com petitor
oligonucleotide containing an ATF binding site can reduce transcription
from the F4 promoter in vitro. This is demonstrated in Figure 4.1.1.
pF4CAT was used as a template for transcription in this assay and a
plasmid containing the major late promoter (pMLP) was included as a
negative control, since the activity of this promoter is not affected by ATF
proteins. Various amounts of the competitor oligonucleotide (ATF-BS)
were added to the transcription reaction immediately before addition of
the template DNA. In this assay, 100 ng of the ATF-BS oligonucleotide
effectively reduces transcription of the F4 promoter, but has no effect on
transcription from the major late promoter.
4.1.2 Analysis of the effect of preincubation of the nuclear extract with the
competitor oligonucleotide on depletion of F4 promoter activity.
Transcription of the F4 promoter at lower amounts of competitor
DNA may be due to the activity of ATF proteins prior to their being
sequestered by the competitor. Preincubation of the nuclear extract with
the competitor oligonucleotide may ensure that upon initiation of the
transcription reaction, all ATF proteins are bound to the competitor DNA
and none to the promoter DNA. This may result in less competitor DNA
being required to reduce transcription and subsequently, the addition of
less recombinant protein being required to restore activity to the promoter.
120
ATF-BS (ng) 0 25 50 100 150 200
E4
MLP
Figure 4.1.1: Transcription of the E4 promoter in vitro is inhibited by the addition of a competitor DNA containing an ATF binding site. Lane 1, transcription reaction containing 0.5 pg pE4CAT and 0.75 pg pMLP as templates for transcription; Lanes 2-6, as Lane 1 but with increasing amounts of a competitor oligonucleotide containing an ATF binding site (ATF-BS) added to the transcription reaction.
No Freine. Freine.
ATF-BS (ng) 0 25 50 100 25 50 100
E4
MLF
Figure 4.1.2: Analysis of the effeet of preineubation of the nuelear extraet with the ATF-BS eompetitor oligonueleotide on depletion of E4 promoter aetivity. Lane 1, transeription reaetion eontaining 0.5 pg pE4CAT and 0.75 pg pMLF as templates for transeription; Lanes 2-4, as Lane 1 but with inereasing amounts of the ATF-BS eompetitor oligonueleotide added at the start of transeription (No Freine.); Lanes 5-6, as Lane 1 but with inereasing amounts of the ATF-BS eompetitor oligonueleotide ineubated with the nuelear extraet at 4°C for 15 min before the start of transeription (Freine.).
Various amounts of competitor were tested for their ability to
reduce transcription of the E4 promoter either with or w ithout
preincubation with the nuclear extract in the absence of promoter DNA.
Figure 4.1.2 shows that preincubation of the competitor DNA with the
nuclear extract has no effect on the amount of competitor DNA that is
required to reduce transcription of the E4 promoter.
4.1.3 The effect of glutathione, thrombin and imidazole upon the in vitro
transcription reaction.
In order to reconstitute the transcriptional activity of the ATF
depleted transcription reaction, recombinant ATF proteins must be added
to the transcription reaction. Two systems for the expression and
subsequent one step purification of recombinant proteins in E. coli
(his-tagged and glutathione-S-transferase (GST) fusion proteins) were
examined for use in this procedure. His-tagged fusion proteins are purified
by binding to a nickel-agarose column and eluting in 250 mM imidazole,
whereas GST fusion proteins are purified by binding to a glutathione-
agarose column and eluting in 5-15 mM glutathione. These fusion
proteins can be cleaved with thrombin to separate the recombinant
protein from its poly-histidine or GST fusion.
I examined the effect of the purification conditions of each of these
systems on the in vitro transcription reaction in order to determine
whether proteins purified in such a way could be added directly to the
transcription reaction, or whether they would require dialysis prior to
their use in the transcription assay.
Figure 4.1.3 A shows that addition of up to 3 mM glutathione does
not adversely affect the transcription reaction, whereas addition of
0.6 units of thrombin (Figure 4.1.3 A) or 25 mM imidazole (Figure 4.1.3 B)
123
Units thrombin mM glutathione
0 0.06 0.2 0.6 0 0.12 0.6 3.0
E4
MLP
Figure 4.1.3 A; Effect of glutathione and thrombin on transcription of the E4 and MLP promoters in vitro. Lanes 1 and 5, transcription reaction containing 0.5 jig pE4CAT and 0.75 pg pMLP as templates for transcription; Lanes 2-4, as Lane 1 but with increasing amounts of thrombin added to the transcription reaction; Lanes 6 -8 , as Lane 5 but with increasing amounts of glutathione added to the transcription reaction.
E4
MLP
mM Imidazole
6 12 25 37 50
Figure 4.1.3 B: Effect of imidazole on transcription of the E4 and MLP promoters in vitro. Lane 1, transcription reaction containing 0.5 pg pE4CAT and 0.75 pg pMLP as templates for transcription; Lanes 2-6, increasing amounts of imidazole added to the transcription reaction.
cause a substantial reduction in transcription. Hence GST-fusion proteins
eluted in 10 mM glutathione can be used directly in this assay, whereas
His-tagged fusion proteins will require dialysis before addition to the
transcription reaction. It is likely that more than 0.6 unit of thrombin
would be added to the transcription reaction when using cleaved fusion
proteins. Hence proteins purified using the His- and GST-tagged fusion
protein systems will be used uncleaved.
4.1.4 Recombinant CREB restores transcription of the E4 promoter by ATE
depleted nuclear extracts.
To restore transcription from the E4 promoter in the ATE depleted
assay, various amounts of a recombinant His-tagged CREB (HôCRla) were
added to the transcription reaction immediately after addition of
competitor and template DNA. Figure 4.1.4 shows that addition of 4.5 |xl
(approximately 500 ng) of HéCRla to the transcription reaction effectively
restores transcription from the E4 promoter.
It is possible that the transcriptional stimulation observed is not due
to the activity of H ôC R la , but due to the activity of ATE proteins
endogenous to the nuclear extract. Rather than activating transcription
directly, H^CRla may be competing with the endogenous ATE proteins for
binding to the competitor DNA, thereby releasing these proteins from the
competition effect and allowing them to stimulate transcription. In an
attempt to establish whether or not this is the case, I examined the effect of
pretreatment with protein kinase A (PKA) upon the activation of
transcription by HôCRla. Phosphorylation of CREB by PKA has been
shown to significantly increase activation of transcription of the
somatostatin promoter by CREB in vitro (Gonzalez et a l , 1991; Yamamoto
et al., 1988). Transcriptional stimulation upon addition of H^CRla and
126
HéCRla+PKA HéCRla PKA
0.5 1.5 4.5 0.5 1.5 4.5 0.5 1.5 4.5 ni
E4
ATF-BS (ng) 0 25 50 100
Figure 4.1.4: Recombinant CREB (HôCRla) restores transcription of the E4 promoter by ATE depleted nuclear extracts, but the CREB dependent stimulation of transcription is not enhanced by protein kinase A (PKA). Lane 1, transcription reaction containing 0.5 pg pE4CAT as the template for transcription; Lanes 2-4, as Lane 1 but with increasing amounts of competitor oligonucleotide (ATF-BS) added to the transcription reaction; Lanes 5-7, as Lane 4 (100 ng ATF-BS) but with increasing amounts of PKA- treated HsCRla added to the transcription reaction; Lanes 8-10, as Lane 4 but with increasing amounts of H éC Rla added to the transcription reaction; Lanes 11-13, as Lane 4 but with increasing amounts of PKA added to the transcription reaction.
PKA to the transcription assay, but not PKA alone, would demonstrate
that the recombinant CREB protein is specifically activating transcription.
Figure 4.1.4 shows that PKA has only a small stimulatory effect on the
activity of H^CRla in this assay. This activation is due to phosphorylation
of H ôCRla and not due to phosphorylation of other components of the
transcription reaction, since addition to the assay of PKA alone has no
effect on transcription.
4.1.5 A mutant recombinant CREB protein, HôCRlAP, cannot restore
transcription of the E4 promoter by ATE depleted nuclear extracts.
To further analyse the effect of adding HôCRla to the transcription
reaction, I examined the activity of a mutant CREB protein (HôCRlAP) that
lacks sequences known to be associated with transcriptional activation.
HeCRlAP has an internal deletion of 134 amino acids that include the
kinase inducible domain (KID; which contains PKA, PKC and casein
kinase II phosphorylation sites; Gonzalez e ta l , 1991; Lee et a l , 1990) and
the alternately spliced a-peptide (Yamamoto et ah, 1990). These domains
have previously been shown to be important for transcriptional activation
by CREB (Gonzalez et ah, 1991; Lee et ah, 1990; Yamamoto et ah, 1990) and
partial deletion of some of these sequences renders CREB transcriptionally
inactive (Gonzalez et ah, 1991; Lee et ah, 1990). Therefore any stimulation
of E4 promoter activity in the ATE depleted transcription reaction after
addition of HôCRIAP will almost certainly be due to the activity of ATE
proteins endogenous to the nuclear extract.
Firstly, I quantified the relative amounts of the two recombinant
CREB proteins that are to be added to the transcription reaction.
Figure 4.1.5 A demonstrates that 5 |xl of H ôC R la gives an equivalent
density band to 5 pi of H^CRIAP when analysed by SDS/PAGE and stained
128
— — 97.4 kDa— 6 6 .2 kDa
— — — 42.7 kDa
J — 31.0 kDa
1 2 3 4 M
H^CRIAP H^CRla
Figure 4.1.5 A: Quantitation of the relative amounts of recombinant CREB proteins. Proteins were run on an 8 % SDS/polyacrylamide gel and visualised by staining with coomassie blue. Lane 1, 5 pi H5 CRIAP; lane 2, 1 pi H^CRIAP; lane 3, 1.0 pi H^CRla; lane 4, 5 pi H^CRla; M, molecular
weight markers.
ComplexH^CRIAP
ComplexH^CRla
Unbound _D N A " —
1 2 3 4 5 6
H^CRIAP H^CRla
F igu re 4 .1 .5 B: Quantitation of the DNA binding activity of recombinant CREB proteins. Various amounts of H ^CRla and H^CRIAP
were incubated with a ^^P-labelled DNA probe containing an ATP binding site. Unbound DNA and protein/DNA complexes are indicated beside the figure. Lane 1, 0.25 pi H^CRla; lane 2, 0.5 pi H^CRla; lane 3, 1.0 pi H^CRla; lane 4, 0.25 pi H^CRIAP; lane 5, 0.5 pi H^CRIAP; lane 6,1.0 pi H^CRIAP.
HéCRla HôCRlAP
0.5 1.5 4.5 13.5 0.5 1.5 4.5 13.5
E4
MLP
ATF-BS (ng) 0 25 50 100
Figure 4.1.5: A mutant recombinant CREB protein, HéCRlAP, cannot restore transcription of the E4 promoter by ATP depleted nuclear extracts. Lane 1, transcription reaction containing 0.5 pg pE4CAT and 0.75pg pMLP as the templates for transcription; Lanes 2-4, as Lane 1 but with increasing amounts of competitor oligonucleotide (ATF-BS) added to the transcription reaction; Lanes 5-8, as Lane 4 (100 ng ATF-BS) but with increasing amounts of H eC R la added to the transcription reaction; Lanes 9-12, as Lane 4 but with increasing amounts of HeCRlAP added to the transcription reaction.
with Coomassie blue. This estimate of the relative concentrations of these
two proteins is confirmed by analysis in a gel mobility shift assay
performed as described in Chapter 3 and explained briefly in the legend to
Figure 4.1.5 B. Figure 4.1.5 B demonstrates that 1 pi of H^CRla binds an
equivalent amount of a ^^P-labelled probe containing the E4 -50 ATF
binding site as 1 pi of HôCRlAP.
Figure 4.1.5 C shows the effect of the addition of these two proteins
to the ATF depleted transcription reaction. As demonstrated in the
previous section, addition of 4.5 pi of H ^CRla results in the efficient
stimulation of E4 transcription. However, addition of an excess of
FîéCRlAP (13.5 pi) has no effect on E4 transcription. This demonstrates that
the stimulatory effect of adding HeCRla to the transcription reaction is not
due to HôCRla competing with endogenous ATF proteins for binding to
the competitor DNA, but is due to HôCRla binding to and activating the
E4 promoter directly.
132
4.2 Discussion.
In vitro transcription of the E4 promoter can be inhibited by the
addition of a specific competitor oligonucleotide containing an ATF
binding site to the transcription reaction (Lee and Green, 1987) and
transcriptional activity can be restored by the subsequent addition of
purified ATF proteins (Hai et a l , 1988a). I have demonstrated that
transcriptional activity can be effectively restored to a transcription assay
depleted of ATF in this way, by the addition of purified recombinant
CREB. In addition, I have demonstrated that this stimulation is specific to
the activity of the recombinant CREB rather than ATF proteins
endogenous to the nuclear extract, since a mutant CREB protein lacking all
known activation domains does not activate transcription in this assay.
Hence this strategy for depleting a transcription reaction of ATF proteins
will be suitable for the development of an in v i tro assay that will
hopefully demonstrate ElA activated transcription of the E4 promoter
mediated by recombinant ATF-2.
Further development of this assay requires the addition of
recombinant ATF-2 to the ATF depleted transcription reaction, but
unfortunately the GST- and His-tagged ATF-2 proteins that I initially
constructed were not efficiently expressed in E. coli. A recent report by
Furlong et al. (1992) determined several parameters that affect the
expression efficiency of proteins cloned into a pET vector. They observed
that the nature of the second amino acid of a protein affects the efficiency
of its expression, and concluded that the rules for protein expression were
complicated and require direct experimentation. I examined the possibility
that amino acids at the N-terminus of ATF-2 could affect its expression by
constructing a His-tagged ATF-2 protein lacking the N-terminal 15 amino
acids and testing its expression in E. coli. This protein was in fact efficiently
133
expressed and purified (data not shown), but this was unfortunately only
achieved immediately prior to the composition of this work and was not
tested in the assays described above.
Further development of this assay such as to demonstrate E lA
dependent activation of the E4 promoter could proceed by either of two
strategies. The simplest approach would be the comparison of activation
by recombinant ATF-2 and ElA together, to that of ATF-2 alone. This
approach demonstrates efficient ElA dependent activation of other
adenovirus promoters (Green e ta l , 1988; Loewenstein and Green, 1989;
Spangler et al, 1987) and hence in the absence of other ATF proteins that
may normally stimulate basal levels of transcription, this approach may be
sufficient to demonstrate activation of the E4 promoter. Alternatively, I
could compare the effect of adding recombinant ATF-2 to an ATF depleted
transcription reaction using nuclear extracts from adenovirus infected
HeLa cells to one using nuclear extracts from uninfected cells. The
possibility that other proteins, in addition to ATF proteins, may be
required for the ATF dependent activation of the E4 promoter by ElA has
been suggested by the contradictory activities of ATF binding sites in
differing promoter contexts (Jones and Lee, 1991; Lee et a l , 1989; Rooney
et a l , 1990 and this thesis). This approach would allow for the possibility
that such unknown activities may not be present in nuclear extracts from
uninfected cells.
Once an in vitro assay has been established that demonstrates
efficient trans-activation of the E4 promoter by ElA, the role of ATF-2
could be further characterised by mutational analysis of the recombinant
protein. In addition, the minimal requirements for this process can be
determined by fractionation of the nuclear extract into increasingly pure
components. This approach would be useful in identifying other proteins
that may be involved in trans-activation by ElA and would also improve
134
the usefulness of other methods of analysis in determining the roles of
proteins involved in this process.
Raychaudhuri et al. (1987) demonstrated that band shifts of the
whole E4 promoter using crude extracts of adenovirus infected HeLa cells
give rise to an extremely complex, unresolved band shift pattern. In
contrast, by using a smaller DNA probe and by performing a simple
fractionation of the extracts, a more defined and discrete band shift pattern
was obtained. Determining the minimal requirements for frflns-activation
of the E4 promoter by ElA in vitro would allow us to perform band shift
analysis upon the whole E4 promoter using the purified components
required for transcriptional activity. In parallel with the transcription
assay, this approach could be used to demonstrate functional interactions
between ElA and other factors (such as ATF-2 and/or TBP) present in the
transcription complex. Further fractionation of the components of this
assay could identify all factors that are required for such an interaction,
thereby further characterising the functional requirements for trans-
activation.
I have already discussed several possibilities that could explain the
conclusion that the DNA binding specificity of ATF-2 in vitro does not
correlate with activation of the E4 promoter in vivo when ATF-2 was
expected to mediate trans-activation by ElA. This conclusion could also be
examined at the functional level to determine whether mutations in the
ATF/E4E binding sites could affect the binding of ATF-2, such as to
interfere with its role in trans-activation by ElA. Such effects may not
necessarily be manifested as a change in the affinity of ATF-2 for these
mutated binding sites and hence would not be observed in the binding
assays employed in Chapter 3. More importantly, this possibility cannot be
addressed in vivo because of the large number of other proteins that
would be available to interact with this site.
135
Chapter 5: Discussion.
5.1 The relative roles of E4F and ATF-2 in trans-activaüon of the
E4 promoter by ElA.
Although the results presented in this study demonstrate that E4F
binding is not necessary for trans-activation of the E4 promoter by ElA,
they do not discount a role for E4F in this process. To date there are no
mutations known to eliminate ATF binding to the E4 promoter without
affecting E4F binding (Rooney et al, 1990) and it is therefore not possible to
determine the role of E4F in the absence of ATF binding. However, the
properties previously described for E4F (Raychaudhuri et al., 1989;
Raychaudhuri et al, 1987; Rooney et al, 1990) indicate that it is critical for
frans-activation by ElA under some circumstances. In addition, a recent
report suggests that trans-activation of the E4 promoter by ElA is primarily
mediated by E4F in HeLa cells (Bondesson et al, 1992).
Bondesson et al. (1992) demonstrated that the non-conserved
C-terminal exon of ElA contains two interchangeable elements, auxiliary
regions 1 and 2 (ARl and AR2), either one of which is required for
efficient CR3 and E4F dependent trans-activation of the E4 promoter in
HeLa cells. Bondesson et al. examined the requirements of these regions of
ElA for activation of the heterologous promoters described by Rooney
et al. (1990). ElA trans-activation of a heterologous promoter mediated by
E4F (RE4PM2) was found to be ARl and AR2 dependent. However,
neither ATF-2 mediated ElA trans-activation nor ElA trans-activation of a
heterologous promoter mediated by ATF (RE4PM4) require the integrity of
these two elements. Hence trans-activation of the E4 promoter by ElA
would appear to be primarily mediated by E4F (and not ATF-2) in HeLa
cells (Bondesson et al, 1992).
136
However, it is possible that the conclusions of Bondesson et al. are
incorrect. By assuming that trans-activation of the heterologous RE4
promoters by ElA (Rooney et al, 1990) represents trans-activation of the
E4 promoter, Bondesson et al. do not take into account the results of this
thesis, which demonstrate that these promoters are not functionally
comparable. Because of the different conclusions drawn from these results
and those of Rooney et al. (1990), it is apparent that the RE4 promoters do
not reflect the activity of ATF/E4F binding sites in the context of the
E4 promoter. Hence the dependence on the ARl and AR2 regions for
fraws-activation of either of these promoters by ElA may also not be
comparable.
However, the function of these regions and their effect on the
activity of ElA is extremely interesting. Ideally, any future examination of
the activity of these regions should be performed using mutated
E4 promoters such as those described in this thesis. Only by comparing the
activity of a mutated E4 promoter that only binds ATF (WE4PM4) with
that of a mutated E4 promoter that only binds E4F, can the nature of ARl
and AR2 dependent activation be determined in the context of the
E4 promoter. However, a mutation that prevents ATF binding without
affecting binding of F4F has not yet been described.
If the results of Bondesson et al. (1992) are correct, why does ATF
not contribute more significantly to fra ws-activation by FI A when it is
evident from my results that the F4 promoter is efficiently activated in the
absence of F4F binding? It is possible that the ATF protein that mediates
fraws-activation by FIA may have a lower affinity for ATF/F4F binding
sites than F4F. Rooney et al. (1990) demonstrated that F4F forms a stable
complex with the F4 promoter, whereas purified ATF forms much less
stable complexes that are rapidly dissociated. Although the precise nature
of the ATF proteins present in the ATF fraction used by Rooney et al. is
137
not known, their study does serve to demonstrate the high affinity that
E4F has for ATF/E4F binding sites in the E4 promoter. The dissociation
rates of E4F and ATF-2 binding to the ATF/E4F binding sites have not
been examined together and the individual analyses reported for ATF-2
(Jones and Lee, 1991 and described in this thesis) and for E4F (Rooney et al,
1990) used different probe DNA, competitor DNA and reaction conditions
and hence, unfortunately, are not comparable.
5.1.1 Problems associated with interpreting the results of this study.
Although the transient expression assay is an extremely useful tool
for defining the activity of a particular promoter, it does not necessarily
truly reflect the activity of the promoter in its natural context, whether a
cellular or viral promoter. This is very obvious when examining the effect
of ElA on the expression of cellular genes. Many plasmid-borne cellular
promoters are induced by ElA in a transient expression assay, whereas
their endogenous cellular counterparts are not. However, such differences
in activity are less obvious when examining the activity of viral
promoters. Mutational analysis of the adenovirus E2A promoter has
demonstrated different requirements for activation in the context of the
viral chromosome to activation of the plasmid-borne promoter in
transfection assays. In transient transfection assays, mutations in any one
of four promoter elements in the E2A promoter (the TAG A motif, the
ATF binding site and the two E2F binding sites) have no effect on ElA
induced transcription. In a stable transfection assay, a mutant promoter
with only the TAG A motif is sufficient for both basal and ElA induced
transcription. However, in its natural context in the viral chromosome,
mutation of any one of these elements substantially reduces both basal and
ElA induced transcription.
138
5.2 Factors affecting the requirements for trans-activation by ElA.
5.2.1 Regulation of other factors by ElA.
Other studies have demonstrated that ElA regulates the activity of
some cellular transcription factors by mediating their phosphorylation by a
cellular kinase (Hoeffler e ta l , 1988; Raychaudhuri et a l , 1989). Hence the
activity of such ElA dependent kinases in a cell may also affect the
transcription factor requirements in mediating trans-activation in a
particular cell type. For instance, if the ElA dependent kinase that is
responsible for the phosphorylation (and thence the increased DNA
binding activity) of E4F is not expressed or active in a particular cell type,
then trans-activation of the E4 promoter by ElA might be preferentially
mediated by an ATF protein in these cells.
Previous reports have demonstrated that ElA is associated with a
60 kDa polypeptide (Harlow et al., 1986) that has since been identified as
hum an cyclin A (Giordano et a l , 1989; Pines and Hunter, 1990).
Subsequent reports have demonstrated that the ElA complex containing
cyclin A also contains a cellular kinase that is related to the cell cycle
control protein (Giordano e ta l , 1991; Kleinberger and Shenk, 1991).
This kinase has since been cloned and is known as (Tsai et a l , 1991)
Interestingly, p3 4 ^ ‘ ‘ 2 kinase has been demonstrated to phosphorylate the
C-terminal domain of RNA polymerase II (Cisek and Corden, 1989), which
is postulated to be involved in the activator dependant stimulation of
transcription (see above).
It is intriguing to speculate as to the role of these proteins in both
normal cellular transcription and in transcriptional and cell cycle control
during an adenovirus infection. In an uninfected cell, it is possible that the
phosphorylation and dephosphorylation of the RNA polymerase II CTD is
a switch that is responsible for the arrest of transcription during M-phase.
139
In the adenovirus infected cell, the association of ElA with a cellular
kinase that can phosphorylate the CTD may allow the adenovirus to
circumvent the normal cellular controls on transcription during DNA
synthesis. In addition, this kinase may be responsible for the
phosphorylation of cellular factors involved in the transcription of
adenovirus genes (such as E4F) either as a result of its normal cellular
activity, or due to a change in context due to its association with ElA.
5.2.2 Dimérisation of ElA inducible transcription factors.
Another factor that is likely to affect the requirements for trans-
activation by ElA is the complement of proteins capable of forming
heterodimers with an ElA inducible transcription factor such as ATF-2
within a particular cell type. The bZlP family of transcription factors also
includes the Fos/Jun family (for review, see; Curran and Franza, 1988) that
bind to the TRE (TPA response element; TGACTCA; Lee et al., 1987b;
Angel et a l , 1987) which differs from the ATF consensus motif by only the
absence of one base pair. Initially, members of the Fos/Jun and ATF
families were regarded as distinct sets of transcription factors that share the
basic dom ain/leucine zipper region, but which have different DNA
binding specificities. However, more recent studies have indicated that
members of each family can form intrafamilial heterodimers through
interaction of their respective leucine zipper regions (Benbrook and Jones,
1990; Hai and Curran, 1991) which can alter the DNA binding specificity of
the proteins (Hai and Curran, 1991). Hence the availability of ATF-2 to
interact with ATF binding sites in the E4 promoter may not only rest upon
the relative abundance of ATF-2 within a particular cell type, but also
upon the nature of the dimerised form of the protein.
140
5.3 The mechanisms of E4F and ATF-2 mediated frans-activation of the
E4 promoter by ElA.
Independent studies have examined the mechanism of ATF-2
mediated fr^ns-activation by ElA and have demonstrated a requirement
for the potential zinc finger motif present at the N-terminus of ATF-2.
(Flint and Jones, 1991; Zu et al., 1991). A recent study has demonstrated
that ATF-a mediates frflws-activation by E lA through a similar
mechanism to ATF-2 (Chatton etal., 1993). ATF-a shares extensive
homology with ATF-2 (Caire et al., 1990), especially in the N-terminal zinc
finger region and like ATF-2, the integrity of this region is essential for
ATF-a to mediate trans-activation by ElA (Chatton et al., 1993). In
addition, ATF-a was found to associate with ElA and mutational analysis
demonstrated that two separate domains of ATF-a, in the N-terminal and
the C-terminal regions, can independently interact with ElA. The zinc
finger of ATF-a was shown to be responsible for the N-terminal
interaction with ElA, but the nature of the domain at the C-terminus that
interacts with ElA has not been elucidated (Chatton et a l, 1993).
Previous studies have suggested that the mechanisms for activation
mediated by either E4F or ATF-2 are different (Liu and Green, 1990;
R aychaudhuri et a l , 1989) and indeed the results of Bondesson et al.
would at first appear to confirm this suggestion since these two proteins
exhibit different requirements for the integrity of the ARl and AR2
regions of ElA (Bondesson e ta l , 1992). However, if the conclusions of
Bondesson et al. are correct, 1 propose that these two mechanisms are not
dissimilar. Previous studies have demonstrated that ElA functions at the
promoter complex in a similar manner to many cellular activators and
mutational analysis of ElA has demonstrated a requirement for the
activation and promoter targeting domains of ElA in CHO cells (Lillie and
141
Green, 1989; Martin e ta l , 1990). Bondesson e t al . confirmed the
requirement for the promoter targeting domain of E lA for trans-
activation of the E4 promoter in HeLa cells (Bondesson et a l , 1992).
Therefore, E4F activity correlates with the requirement for the promoter
targeting domain of E lA and would be predicted to mediate trans-
activation by ElA via a similar mechanism to that proposed for ATF-2
(Liu and Green, 1990). A direct interaction between E4F and ElA has not
previously been postulated since it has been assumed that stimulation of
the DNA binding activity of E4F by ElA would account for the ElA
induced increase in E4 promoter activity (Raychaudhuri et a l, 1989).
This revised model for the mechanism of E4F activity and the
conclusions of Bondesson et al. are mutually inclusive. E4F is only
predicted to function by recruiting ElA to the E4 promoter if trans-
activation of the E4 promoter by ElA is indeed primarily mediated by E4F.
If E4F does not function by such a mechanism, then conversely it is
unlikely that the observed AR1/AR2 dependent activation of the
E4 promoter is primarily mediated by E4F.
The suggestion that E4F functions to recruit ElA to the promoter
complex also presents a possible function for ARl and AR2. These two
domains are adjacent to the promoter targeting domain of ElA (Lillie and
Green, 1989) and could represent a functional extension of the activity of
this domain. Whereas the previously defined boundaries of the promoter
targeting domain may be a universal requirement for the interaction of
ElA with several transcription factors (such as ATF-2 and E4F), ARl and
AR2 may cooperate with this domain in a manner specific to an
interaction with E4F, but may not be required for the interaction with
ATF-2.
142
5.4 Other examples of multiple factors binding to individual promoter
elements.
The ATF/E4F binding sites in the E4 promoter are not the only
known example of a composite promoter elem ent consisting of
overlapping binding sites for distinct factors. Analysis of factors that bind
to the herpes simplex virus thymidine kinase promoter identified two
CCAAT binding activities, CTF and C/EBP (Jones et a l , 1985; Graves et al.,
1986). Although these proteins both interact with the CCAAT element,
they exhibit slightly different DNA binding specificities. A C-to-G
mutation at the first residue of the CCAAT sequence has opposing effects
on the binding of these two factors, increasing the affinity of C/EBP
(Graves et al., 1986) whilst reducing the affinity of CTF (Johnson and
McKnight, 1989). Subsequent cloning of C/EBP and CTF has demonstrated
that they are encoded by different genes, and somewhat surprisingly, that
they utilise different DNA binding motifs (Landschulz et a l , 1988; Santoro
et al., 1988). Together with the more recent cloning of a family of bZIP
proteins that are closely related to C/EBP, this situation is highly
analogous to that of ATF and E4F binding to the E4 promoter. Whether
E4F is a bZIP protein or utilises a different DNA binding domain will be
revealed when E4F is cloned.
This example demonstrates that the functional redundancy
characterised by multiple transcription factors binding to the E4 promoter
is not specific to viral promoters but is also common to cellular promoters.
This is consistent with the general structure of cellular and viral
promoters demonstrating essential similarities, together with the original
hypothesis that the regulation of viral transcription would reflect cellular
modes of regulation.
143
5.5 The role of ATF proteins in other viral frans-activation pathways.
The results of this and previous studies investigating the roles of
cellular transcription factors in viral trans-activation suggest that the ATF
family is commonly targeted by viral trans-activators in the activation of
viral and cellular genes. In addition to the adenovirus early promoters,
ATF binding sites are also present in the long terminal repeat (LTR) of
human T-cell leukaemia virus type 1 (HTLV-1) and the promoters of the
Krox-20 and Krox-24 genes (Giam and Xu, 1989; Alexandre et a l , 1991).
Recent studies have demonstrated that binding of CREB to the LTR is
enhanced by the HTLV-1 trans-activator, p40 ® , via a direct interaction
between the two proteins (Zhao and Giam, 1992). This is similar to the
mechanism by which CREB and ATF-2 are proposed to mediate activation
of the hepatitis B virus (HBV) enhancer by the HBV X gene product, pX
(Maguire et a l, 1991; see Chapter 3.2). Therefore in at least three distinct
viral systems, ATF proteins have been proposed to mediate tran s
activation by a direct interaction with the viral trans-activator. In addition
to p40 ^ , the bovine leukaemia virus (BLV) trans-activator p38‘® and the
cytomegalovirus (CMV) tegument protein pp71 also target promoters
containing ATF binding sites (Willems et a l , 1992; Hunninghake et a l ,
1989). Future studies will hopefully determine whether ATF proteins are
involved in mediating activation by these viral trans-activators and if so,
the mechanisms involved.
The use of similar pathways by unrelated viruses in the activation
of viral genes is probably a reflection of the mechanisms by which cellular
signal transduction pathways operate. Cellular equivalents of the viral
trans-activators are likely to be adaptor proteins (see Chapter 1.4.3); the
suggestion that these proteins function by bridging and/or stabilising
interactions between transcription factors and the general factors is similar
144
to the proposed mechanism of activation by ElA and other viral trans-
activators. However, whether these factors can activate transcription when
tethered to a DNA binding domain (as has been shown to be the case with
ElA; Lillie and Green, 1989) has yet to be deduced. Once the cellular
equivalent of ElA has been identified, the role of this factor can be studied
in relation to which cellular genes it regulates and what regulatory
pathway it is part of.
145
5. 6 Conclusions.
Together with studies of other viral systems, the study of trans-
activation of adenovirus early genes by ElA has identified many cellular
transcription factors and provided insights into how the cell modulates
transcription. However, the mechanisms by which E lA achieves the
regulation of early gene expression have proved to be more numerous
and varied than was initially expected. The characterisation of the early
viral genes led to the identification of the ATF family and it was
considered likely that ElA utilised a common mechanism, via ATF
binding sites, to activate these promoters. However, we now know that
trans-activation of the E2A promoter is mediated by the E2F transcription
factor, by a mechanism distinct to ATF mediated activation of the
E4 promoter (Hardy and Shenk, 1989; Hardy et al., 1989; Bagchi et ah, 1990).
In addition, the characterisation of E4F as an ElA inducible transcription
factor distinct from the ATF family has further demonstrated the diversity
of ElAs activity.
By targeting multiple transcription factors involved in the
regulation of adenovirus genes, ElA potentially increases the host range of
the virus such that it can efficiently replicate in a wide variety of cell types.
It is likely that the requirements for this process are a complex
combination of several factors, relating not only to the availability of an
individual transcription factor in a particular cell type, but also on the
availability of other proteins involved in trans-activation mediated by that
transcription factor. The different processes by which ElA activates
transcription can be directly related to normal cellular modes of regulation
and hence other factors required for trans-activation by ElA are likely to
also be involved in cellular signal transduction pathways.
146
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Appendix: Abbreviations not described in the text.
Ad2 (Ad5) adenovirus type 2 (type 5)AMV avian myeloblastosis virus
ATP adenosine-5'-triphosphatebp base pairs
cDNA complementary DNACHO Chinese hamster ovary
C-terminus (-terminal) carboxy-terminus (-terminal)CTP cytidine-5’-triphosphate
dATP 2'-deoxy-adenosine-5'-triphosphate
dCTP 2’-deoxy-cytidine-5'-triphosphateDEPC diethyl pyrocarbonatedGTP 2'-deoxy-guanosine-5'-
triphosphateDNA deoxyribonucleic acid
DNase I deoxyribonuclease IDTI 1,4-dithiothreitol (Cleland's
reagent)EDTA ethylenediaminetetraacetic acidEtOH ethanolGTP guanosine-5’-triphosphate
Hepes N-[2-hydroxyethyl] piperazine- N'-[2-ethanesulfonic acid]
His histidinelie isoleucine
IPTG isopropyl p-D- thiogalactopyranoside
kb kilobaseskDa kilodaltonsLeu leucine
MgOAc magnesium acetateNaOAc sodium acetate
NH 4 OAC ammonium acetateN-terminal (-terminus) amino-terminal (-terminus)
PAGE polyacrylamide gel electrophoresis
168
PBS phosphate buffered saline PEG polyethylene glycol Phe phenylalanine
PIPES piperazine-N,N'-bis [2-ethane- sulfonic acid]; 1,4-piperazine- diethanesulfonic acid
PMSF phenylmethylsulfonyl fluoride pol II RNA polymerase II
Pro proline RNA ribonucleic acid
RNasin ribonuclease inhibitor rRNA ribosomal RNA
SDS sodium dodecyl sulfate Ser serine
TEMED N,N,N',N'-tetramethylethylene- diamine
Thr threonineTLC thin layer chromatography Tris tris[hydroxymethy 1]amino-
methane tRNA transfer RNA
TIP thymidine-5'-triphosphate Tyr tyrosine
UTP uridine-5'-triphosphate U.V. ultraviolet
169
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