koch’ postulates 병원균과 질병의 지속적 연관성 분리, 순수배양 건강한...
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Koch’ Postulates
• 병원균과 질병의 지속적 연관성• 분리 , 순수배양• 건강한 개체에 접종시 동일한 증상• 재분리 후 동일한 병원균 확인
Plant vs. Animal
• No antibody, T cell response• No circulating cell• Systemic signal through plant• Disposable and replaceable parts in
plant
Gene for Gene theory
• Avr : avr (avirulence) gene• R : r (resistance) gene
• R * Avr = Resistance• R * avr = Susceptible• r * Avr = Susceptible• R * avr = Susceptible
Signal Molecules
• ROD, reactive oxygen species• NO, nitric oxide• SA, salicylic acid• JA, jasmoic acid• C2H2, ethylene
신호전달 후• HR, hypersensive response• Phytoalexins• PR, pathogenesis related proteins• Cell wall reinforcing, H2O2
• Ca++
• Active oxygens• SA
R and Avr interactions
• PAMP(pathogen associated molecular pattern) through TTSS(type III secretion system), Fls2(flagellin receptor)
• AvrRpm1 increases phosphorylation of Rin4 and decrease PAMP signaling
• AvrRpt2, protease cleaves Rin4
• Rpm1 interact with Rin4 and detect change in Rin4
내병성 육종Exogeneous genes
- pathogen origin (M, Coat proteins, replicase) - plant origin (RIP)
Endogenous genes - SAR - HR - PR - Signal transduction
내병성 분자육종• Engineering of R for broad spectrum• Expression of R in an inducible manner - broad specificity of pathogens - multifactorial defense mechanism - only w/I infected tissue
• 내병성 육종의 한계 : 병원균의 진화
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