metagenomics rob edwards mcs. the soudan mine, minnesota red stuff oxidized black stuff reduced
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Metagenomics
Rob Edwards
MCS
The Soudan Mine, MinnesotaRed Stuff OxidizedBlack Stuff Reduced
Red and Black Samples Are Different
Cloned and 454 sequenced16S are indistinguishable
Black stuff
Red
ClonedRed
There are different amounts ofsubstrates in each environment
BlackStuff
RedStuff
There are different amounts of metabolism in each environment
Nitrification differentiates the samples
Edwards (2006)
The SEED metagenomics server
Metabolic potential in environments
Phylogenomics
Comparing Metagenomes to Genomes(or other metagenomes!)
Lots of sequencesall pyrosequencing
Metabolic potential in environments
Sulfur
CDA 60.2%
CD
A 2
1.7
% Respiration
Capsule Motility
Membranetransport
Stress
Signaling
Phosphorus
RNA
MineSaltern
MarineMicrobialites
CoralFish
AnimalsFreshwater
From sequences to environments
Dinsdale et al. unpublished
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