phage status report #2

PHAGE STATUS REPORT #2

Anna Phipps and Janna Anderson

PHAGE NAME AND REASONING

We decided name our phage Banana7 because Banana rhymes with both our names.

SOURCE OF OUR PHAGE

We got our phage from Dr. K’s clean compost sample

PLAQUE MORPHOLOGY

i. Size: 1.5 mm

Ii. Appearance: Our phage is clear without a halo

TITER OF HTL

The titer of our HTL is 1.95*10 ^7

DNA ISOLATION

A. Concentration: 1.95µg/µl

B. Yield: 19.5µg

C. : 0.01733

D.: Restriction Digest Results: PstI:2cm, SalI:2cm, EcoRv: 2cm, NcoI:3cm,

2ND DNA GEL RESULTS

Our second round of using restricting enzymes on our gel was very faint, but the results were interesting.

Controle: 1cm, BanHl:0.5cm, Clal: 1cm, EcoRl:1cm, Haell &Hindll: 1cm.

DOES YOUR P HAGE LOOK L IKE ANY OTHER PHAGES FROM THE CLASS OR

DATABASE?

As far as we have looked, our phage does not look like any others from the class or the database.

A N Y T H I N G E L S E T H AT I S I N T E R E S T I N G A B O U T Y O U R P H A G E ?

Our phage is a siphoviridae. It looks like the head has been broken into and it is now

dead.

WHY SHOULD THE CLASS SELECT YOUR PHAGE AS THE ONE TO

SEQUENCE?

I would say that our phage should be picked because of its clarity and awesome name.