restriction enzymes

Describe digestion of DNA by

LearningOutcomes:1.DefineandGiveexamplesofRestrictionEndonucleases.

2.ExplainhowrestrictionenzymesproduceRecombinantDNA.

DNA Manipulation:

Enzymescalledrestrictionendonucleasesrevolutionisedmolecularbiologybecauseoftheirabilityto“cut”DNAatspecificsites.

ButHowDOrestrictionenzymeswork?

Theyallowthecreationofrecombinantmolecules;theseenzymesrecogniseaspecificDNAsequence,rangingfromandcuttheDNAataspecificbasewithinthissequence.

TheRecognitionsitesformanyRestrictionEnzymesarepalindromes...

Apalindromeisawordorphrasethatreadsthesameforwardandinreverse,e.g...

SO!...ThepalindromicDNAsequencereadsthesamefrom5'to3'ononestrand

asitdoesonthecomplementarystrand.

CuttingtheDNAatthesamebaseoneitherstrandproducesstaggeredcutsCalled“stickyends.”

SO,These“stickyends”allowDNAfromdifferentsourcestobeeasilyjoinedtogether!

Theseshort,unpairedsequencesarethesameforanyDNAthatiscutbythisenzyme...

Describe this Diagram!! Work in pairs, You have 5 minutes to provide a step by step, explanation of what it is going on! (use the animation to help!)