rna polymerase binding
Post on 31-Dec-2015
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RNA Polymerase binding
*Constitutive or basal level transcription
Allosteric activation of RNA polymerase
Inhibition of transcription
DNA-protein interactions
Negative Control of the lac Operon
Proposed CAP-cAMP Activation of lac Transcription
S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929
Three major classes of RNA:
Transcription in Eukaryotes
Type Encodes Copies/genome % Total RNA
mRNA proteins Single or few 10%
tRNA functional RNA Few 20%
rRNA functional RNA Many 70%
But there are more!!!
Epitope Tagging of RNA Polymerase II
*Genetically “tag” Polymerase II subunit
*Grow cells in radioactively labeled amino acids
*Allow assembly of active polymerase
*Add antibody that binds epitope
*”Grab” antibody and what ever it is bound to
*SDS electrophoresis
Transcription*RNA polymerase II
Transcription*RNA polymerase II
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