time kill assay (mic calculations)

7/23/2019 Time Kill Assay (Mic Calculations)

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TIME KILL ASSAY 

Bacteria

MIC (1% concentration)  

0 MIC = no extract added (1000μL MHB primer)0.5 MIC = μg/mL1 MIC = μg/mL2 MIC = μg/mL MIC = μg/mL

! MIC = μg/mL

0.5 MIC = μ g/mL

M1 = 10000 μg/mL"1  = #M2 = μg/mL"2  = 1000 μL

(10000 μg/mL)( "1) = ( μg/mL)( 1000 μL)

"1 = μL

  μL (extract $o&me) ' μL (MHB

primer)

1 MIC = μ g/mL

M1 = 10000 μg/mL"1  = #M2 = μg/mL"2  = 1000 μL

(10000 μg/mL)( "1) = ( μg/mL)( 1000 μL)

"1 = μL

  μL (extract $o&me) ' μL (MHBprimer)

2 MIC = μ g/mL

M1 = 10000 μg/mL"1  = #M2 = μg/mL"2  = 1000 μL

(10000 μg/mL)( "1) = ( μg/mL)( 1000 μL)

"1 = μL

  μL (extract $o&me) ' μL (MHBprimer)

MIC = μ g/mL

M1 = 10000 μg/mL"1  = #

M2 = μg/mL"2  = 1000 μL

(10000 μg/mL)( "1) = ( μg/mL)( 1000 μL)

"1 = μL

  μL (extract $o&me) ' μL (MHBprimer)

! MIC = μ g/mL

M1 = 10000 μg/mL"1  = #M2 = μg/mL"2  = 1000 μL

(10000 μg/mL)( "1) = ( μg/mL)( 1000 μL)

"1 = μL

  μL (extract $o&me) ' μL(MHB primer)