and

UW Chemistry Faculty Directory (pp. 67-83)

Friday, September 29, 2017

Wisconsin Room, UWM Student Union Map p. 88/89

4:30 – 7:00 pm Check-in 5:00 pm Poster Session pp. 11-66

6:30 pm Opening Remarks and Dinner 7:30 pm Plenary Address p. 5

8:30 – 10:00 pm Mixer

Saturday, September 30, 2017

Kenwood Interdisciplinary Research Complex (KIRC) Map p. 88/90

7:30 am Chairs Breakfast

9:00 am Coffee/Snacks 9:15 am Workshop Concurrent Sessions I p. 10

10:15 am Break

10:30 am Workshop Concurrent Sessions II p. 10

11:30 am Lunch & Luncheon Keynote Address p. 6

Sponsored by

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Department of Chemistry & Biochemistry 29th September 2017

Welcome UW Chemists,

It is with enthusiasm that I welcome you to the 44th UW-System Chemistry Faculties Meeting.

A sturdy 44-year record is testament to the importance of this conference and the conviction of the UW Chemistry faculty. UW-Milwaukee Chemistry and Biochemistry is very pleased to host this event for 2017 and our organizing committee has worked diligently to make this years’ meeting noteworthy and enjoyable for all attendees. Of particular note, we are very fortunate to have a current Nobel Laureate in Chemistry offer the Friday night plenary address. Ben Feringa has rightly garnered acclaim for his work with stereochemistry, organic synthesis, asymmetric catalysis, having been awarded the Koerber European Science Award (2003), the Spinoza Award (2004), the Prelog gold medal (2005), the Norrish Award of the ACS (2007), the Paracelsus medal (2008), the Chirality medal (2009), the RSC Organic Stereochemistry Award (2011), Humboldt Award (2012), the Grand Prix Scientifique Cino del Duca (French Academy 2012), the Marie Curie medal and the Nagoya Gold Medal (2013) and of course most recently the Nobel (2016). The Nobel prize was specifically bestowed for his work with self-assembling molecular switches, motors and molecular machines, that will of course be the subject of his lecture. The conference is indebted to the generosity of our sponsors who have made it possible for each attendee to bring a guest to the Friday evening dinner, plenary and mixer; a particularly appropriate concession given the importance of the plenary address. Shimadzu Scientific, Eurofins and UW-System are the primary donors for this years’ conference, but we have also received significant support from TWD Tradewinds, ThermoFisher, Vernier, Bruker, Waters, Turningpoint Technologies, Pearson, Tophat, Macmillian and McGraw Hill. UW-system’s contribution has been specifically put toward the Saturday workshops and exhibitions that will occur in the new KIRC building. We have tried to make the workshops relevant to all by including discussions of specific professional considerations for UW chemistry faculty, pedagogy and strategies for education, along with current instrumentation and techniques. The Saturday morning luncheon address will be given by Kyle Swanson who spear-headed efforts at UWM in program alignment and retention of at-risk students. His lecture will describe how to reach and target cohorts of students for tertiary education successes.

It is the sincere desire of the Chemistry and Biochemistry faculty that you have an enjoyable, enriching, and relevant experience at the 2017 UW-System Chemistry Faculties Meeting. We acknowledge that many of you have travelled considerable distances to gather with us in Milwaukee, and hope that you depart the event with a sense of renewed connection to your system colleagues and re-invigorated within the chemistry education discipline.

Dr Graham R. Moran Chair, Department of Chemistry and Biochemistry University of Wisconsin-Milwaukee

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Eurofins is a global network of laboratories specializing in chemical, bioanalytical, microbiological,

and physical testing services. We offer solutions for both routine analysis and specialized testing

and project management. Our technical expertise ensures the highest quality results and

reporting, and our scientists are here to assist with your project challenges.

Eurofins was founded in 1987 by Gilles Martin in France with 4 people having a focus on food

authenticity and quality. Since then the company has grown to over 30,000 staff in more than

375 laboratories with over 130,000 methods across 41 countries. In the United States alone,

there are 39 laboratories serving the needs of industry, research institutions, and academia.

Our services include testing for food, pharma, environmental, consumer products, and food

safety training. The Eurofins path has been and continues to be fulfilling the mission “To

contribute to global health and safety by providing our customers with high quality laboratory

and advisory services…”

The Eurofins S-F Analytical laboratory in New Berlin, WI continues to enjoy a close relationship

with the UW system. Included in our staff of chemist/scientists are 5 chemists with degrees from

UW-M and 4 others in senior chemist and managerial positions having degrees from UW

programs. A talented group of individuals comprised of local and national sources allows us to

excel at providing testing services in Food Chemistry, Specialty Environmental, Microbiology and

Special Investigative Analysis.

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We greatly appreciate support for the meeting from the Office of the Vice President for Academic & Student Affairs, UW System.

The University of Wisconsin System is one of the largest systems of public higher education in the country, serving approximately 180,000 students each year and employing more than 39,000 faculty and staff statewide.

The UW System is made up of 13 four-year universities, 13 freshman-sophomore UW Colleges campuses, and statewide UW-Extension. Together, these institutions are a tremendous academic, cultural, and economic resource for Wisconsin, the nation, and the world.

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Plenary Address

Friday, September 29, 2017 at 7:30 pm Wisconsin Room, UWM Student Union

The Art of Building Small

Bernard L. Feringa Nobel Laureate in Chemistry 2016

Jacobus van't Hoff Distinguished Professor of Molecular Sciences Stratingh Institute for Chemistry

University of Groningen, Netherlands

Beyond the fascinating designs of nature, the creative power of synthetic chemistry provides unlimited opportunities to build our own molecular world. For centuries, chemists have built their own molecular tools to create the building blocks of this world, which in turn enabled them to create life-sustaining drugs, smart materials, and molecules that have shaped modern society. In their art of building small, chemists have changed the macroscopic world; however, some have taken on the fundamental challenge to understand the principles of dynamic molecular systems to gain control of molecular motion at the nanoscale. In my presentation, I will describe our journey in the world of molecular switches and motors in addition to our contributions to control the regio- and stereochemistry of organic reactions with the aid of homogeneous catalysts. Prof. Ferigna is the Jacobus van’t Hoff Distinguished Professor of Molecular Sciences, Stratingh Institute for Chemistry, University of Groningen, Netherlands. Among his many honors, he was awarded: the Koerber European Science Award (2003), the Spinoza Award (2004), the Prelog gold medal (2005), the Norrish Award of the ACS (2007), the Paracelsus medal (2008), the Chirality medal (2009), the RSC Organic Stereochemistry Award (2011), Humboldt Award (2012), the Grand Prix Scientifique Cino del Duca (French Academy 2012), the Marie Curie medal and the Nagoya Gold Medal (2013), and the Nobel Prize in 2016. The Nobel Prize was specifically bestowed for his work with self-assembling molecular switches, motors and molecular machines.

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Luncheon Keynote Address

Saturday, September 30, 2017 at 11:30 am KIRC 1150

Doubling completion rates: Impact of acceleration and intensification in early career STEM education for at-risk students

Kyle Swanson

Dean, College of Sciences Metropolitan State University

St. Paul, MN Retention of academically at-risk students to major and degree in STEM fields is challenging for a number of reasons, most notably extensive pre-requisite course sequences necessary for entry into upper-level courses and difficulties in completing mathematics requirements. Here we describe the impact of an intense early-college STEM cohort experience on introductory STEM course sequence completion rates for academically at-risk STEM-intended first-year students, where at-risk is defined as placement into high-school level algebra and chemistry courses. Students in the cohort took introductory mathematics, chemistry and biology courses as co-requisites, participated in a professional development/academic support seminar, and had extensive access to senior faculty during their first year. In both quasi-experimental and limited randomization studies, students in the cohort completed introductory major-level course sequences in biology and chemistry at roughly double the rate of students in the control groups. This suggests that early career academic intensification and acceleration through co-requisite models may provide a means to keep more students in STEM majors. Kyle Swanson, PhD, is the Dean of the College of Sciences at Metropolitan State University. Prior to joining Metropolitan State, he held the positions of Professor, Department Chair and Associate Dean in the College of Letters and Science at the University of Wisconsin-Milwaukee. He holds Bachelor’s degrees in mathematics and physics, magna cum laude with honors, from St. Olaf College in Northfield, MN, and a PhD in Geophysical Sciences from the University of Chicago. For two decades he has been engaged in theoretical and observational research on the fluid dynamic character of the atmosphere, emphasizing variability on time scales ranging from days to years. He also held postdoctoral appointment at the Geophysical Fluid Dynamics Laboratory in Princeton, NJ and was a NSF/NATO fellow at the Laboratoire de Météorologie Dynamique at the École Normale Supérieure in Paris, France. He is the author or coauthor of over 40 peer-reviewed scientific articles, appearing in such journals as Science, Proceedings of the National Academy of Sciences and Physical Review Letters. He has given over 50 interviews and talks, including presentations at MIT, University of Illinois, University of Wisconsin, and the California Institute of Technology. At UW-Milwaukee he was instrumental in the implementation of remedial mathematics reform at institutional scale, as well as integrating early college career experiences for STEM majors. For these and other activities he was awarded the UW-Milwaukee Distinguished Faculty University Service Award in 2016.

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The 44th Annual UW System Chemistry Faculties Meeting

Exhibitors

Bruker BioSpin is the market leader in analytical research tools based on magnetic resonance. Our comprehensive portfolio includes NMR, EPR and TD-NMR, delivering a range of research tools to enable life science, materials science, analytical chemistry and process control. For more information please visit www.bruker.com.

Macmillan Learning brings together some of the most respected imprints in Chemistry Education to enhance the classroom and lab. Learn how we partner with thought leaders in chemistry education to produce the best in scientific publishing from W.H. Freeman, Roberts & Company, Hayden-McNeil, Sapling Learning, and Late Nite Labs. www.macmillanlearning.com/chemistry

Personalize learning with MasteringChemistry. MasteringChemistry is the leading online homework, tutorial, and engagement system, designed to improve results by engaging students with vetted content. The enhanced eText 2.0 and MasteringChemistry work with the book to provide seamless and tightly integrated videos and other rich media and assessment throughout the course. Instructors can assign interactive media before class to engage students and ensure they arrive ready to learn. Students further master concepts

through book-specific MasteringChemistry assignments, which provide hints and answer-specific feedback that build problem-solving skills. With Learning Catalytics™ instructors can expand on key concepts and encourage student engagement during lecture through questions answered individually or in pairs and groups. MasteringChemistry now provides students with the new General Chemistry Primer for remediation of chemistry and math skills needed in the general chemistry course.

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Kemtech America has been marketing exclusively Synthware brand specialty glassware in North America for over 20 years. Synthware is one of the premium bands, recognized for its quality and availability in synthetic chemical laboratories. While our products are available through all major laboratory product distributors, we also supply directly to universities and research laboratories directly from warehouse in Wisconsin. Additional information including our competitive online pricing are available on our website at www.kemtech-america.com.

Thermo Fisher Scientific’s Laboratory Chemicals Division is a leading chemical provider serving scientific laboratories and production customers globally. With a strong global manufacturing footprint and secure supplier partnerships, our world class brands deliver convenience, quality, and safety. Our team of knowledgeable sales professionals and chemical experts collaborate with researchers to

provide comprehensive scientific consultation, simplifying the day-to-day duties of lab supplies management. All to help our customers reach their goal of making the world healthier, cleaner, and safer.

Top Hat's interactive, cloud-based teaching platform enables professors to engage students inside and outside the classroom with compelling content, tools and activities. Millions of students at 700 leading North American colleges and universities use the Top Hat teaching platform. To learn more, visit www.tophat.com.

Turning Technologies interactive solutions are designed to support proven learning theories and help enhance learning outcomes. Seamlessly poll with PowerPoint®, over any application, on the web or conduct self-paced

assessments. Collect student responses through the use of web-enabled mobile devices or clickers and gather detailed reports. www.TurningTechnologies.com.

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Vernier Software & Technology has been an innovator of data-collection technology for over 30 years. Creating easy-to-use and affordable science interfaces, sensors, and software, their products can be found in education in over 125 different countries. Vernier helps teachers enhance their science

curriculum, increase learning, and build students' critical thinking skills. For more information visit www.vernier.com.

Waters Corporation is a supplier of separations equipment that includes HPLC, UHPLC, UPLC, and SFC (UPC2) systems. They also provide a complete line of mass spectrometers, including a low cost single quad, a full featured single quad, various

tandem (triple) quads as well as a full line of TOF and qTOF instruments. Informatics solutions include chromatography data systems, sample management systems, electronic lab notebooks and mass spectrometry software. Waters also has a full line of columns and supplies. Kevin McKaveney, the Madison based Account Manager, can be reached at 1 800 252-4752 x6035 or by emailing kevin_J_mckaveney@waters.com.

The Graduate School embodies the Chancellor’s graduate education vision to maintain UWM’s status as a top-tier doctoral research university that is the best place to learn and work for graduate students, postdoctoral scholars, and graduate faculty. UWM offers 135+ master’s, doctoral, professional, and graduate certificate programs, more than any other university in Southeastern Wisconsin. Contact: gradschool@uwm.edu or (414) 229-6569.

The Milwaukee Institute for Drug Discovery (MIDD) advances research and later-stage development of new drugs from research at the UW-Milwaukee and collaborating institutions. The MIDD focuses on several areas of research

strength at UWM; with initiatives in neuroscience, cancer, and infectious diseases. The MIDD established the Shimadzu Laboratory for Advanced Applied and Analytical Chemistry to support drug discovery research and a wide range of academic and industrial collaborative programs. Visit http://uwm.edu/drug-discovery or contact dcstaff@uwm.edu.

Graduate School

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The 44th Annual UW System Chemistry Faculties Meeting

Workshops

Saturday, September 30, 2017 Kenwood Interdisciplinary Research Complex (KIRC)

Theme Room Session I 9:15 am Session II 10:30 am

Education KIRC 1140 Classroom and programmatic assessment / ACS Exams

Program coordination and alignment between secondary and post-secondary institutions

Research Instrumentation

KIRC 1160

Advanced methods for protein separations, offered by Shimadzu Scientific Instruments

Instructional laboratory equipment and instrumentation including new wireless sensors, offered by Vernier

Faculty Development and Collaboration

KIRC 1130 Workload policy and post-tenure review

Undergraduate program development and retention efforts

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The 44th Annual UW System Chemistry Faculties Meeting

Poster Session

Friday, September 29, 2017 at 5:00 pm Wisconsin Room, UWM Student Union

List of Presenters

Poster Presenter Affiliation

001 Mohamed Ayoub UW-Washington County

002 Jamie L. Schneider UW-River Falls 003 Pamela Doolittle UW-Madison

004 Victoria Fisher UW-Milwaukee 005 Lamia Tabassum Badhon UW-Milwaukee

006 Joseph V. Roman UW-Milwaukee 007 Matthew R. Hoag UW-Milwaukee

008 Afsana A. Mahim UW-Milwaukee

009 Heli Fan UW-Milwaukee 010 Jennifer E. Mihalick UW-Oshkosh

011 Eric D. Lund UW-Milwaukee 012 Jaclyn Trate UW-Milwaukee

013 Thao Yang UW-Eau Claire

014 Bassam Z. Shakhashiri UW-Madison 015 Amanda N. Nieman UW-Milwaukee

016 Nemanja Vuksanovic UW-Milwaukee

017 Lisa Kroutil UW-River Falls

018 Oliva Yu UW-Milwaukee

019 Margaret L. Guthrie UW-Milwaukee

020 Tania Mutchie UW-Milwaukee

021 Daniel E. Knutson UW-Milwaukee

022 Brandon Patterson UW-Milwaukee

023 Guanguan Li UW-Milwaukee

024 Joseph T. Labeots UW-Milwaukee 025 Mizzanoor Rahaman UW-Milwaukee

026 Yujuan Liu UW-Parkside

027 Md Toufiqur Rahman UW-Milwaukee

028 Joseph H. Aldstadt UW-Milwaukee

029 David Koltermann UW-Milwaukee

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030 Mahbbat Ali UW-Milwaukee 031 Sarah A. Oehm UW-Milwaukee

032 Md Shadiqur Rashid Roni UW-Milwaukee

033 Farjana Rashid UW-Milwaukee

034 Revathi Kodali UW-Milwaukee

035 V. V. N. Phani Babu Tiruveedhula UW-Milwaukee 036 Gloria S. Forkuo UW-Milwaukee

037 Lanlan Han UW-Milwaukee

038 Alec Huber UW-Milwaukee

039 Shamsul Arefin Ahmed UW-Milwaukee

040 Elliot Di Milo UW-Milwaukee 041 Rajwana Jahan UW-Milwaukee

042 Liana Lamont UW-Madison

043 Anja Blecking UW-Milwaukee

044 Bridgette Walters UW-Milwaukee

045 Curtis J. Czerwinski UW-La Crosse 046 Tyler Fenske UW-Milwaukee

047 Zechao Lin UW-Milwaukee 048 Rob McClain UW-Madison

049 Dana C. Haagenson UW-Marshfield/Wood County 050 Ilia A. Guzei UW-Madison

051 Xavier S. Udad UW-Milwaukee

052 Cheri A. Barta UW-Madison 053 Quintus Owen UW-Milwaukee

054 Yiming Yang UW-Milwaukee

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POSTER 001

Natural Bond Orbitals and Hydrogen Bonding

Mohamed Ayoub

Department of Chemistry, University of Wisconsin-Washington County We employ hybrid density functional theory in augmented correlation, consistent triple-zeta basis level (B3LYP/aug-cc-pVTZ) performed with Gaussian09 program and analyzed with Natural Bond Orbitals program (NBO 6.0), to confirm that charge- transfer (QCT) is a primary reason for

the formation of hydrogen bonding (B…HA). A wide variety of B…HA dimers: normal (HB),

isomeric, charge-assisted (CAHB), pi-denoting (-HB) and anti-electrostatic (AEHB) were examined. The conclusion is supported by the good correlation between NBO theoretical

descriptors such as: QCT (e), stabilization energy (EQCTkcal/mol), NRT bond orders (bB…H,bHA) and structural and energetic signature known to be associated with the formation of hydrogen

bonding such as: hydrogen bond energy (EHB,kcal/mol), elongation of HA bond (RHA, Å), and

IR frequency shifts (HA,cm-1).

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POSTER 002

Delayed answer-until-correct general chemistry test feedback improves repeat performance more than passive reading of correct answers after chemistry test

Jamie L. Schneidera, Kristen L. Murphyb, Panayiota Kendeouc, Shalini Srinivasanb,

and Arunendu Chatterjeed

Departments of Chemistrya and Mathematicsd, University of Wisconsin River Falls; Department of Chemistry and Biochemistryb, University of Wisconsin Milwaukee;

Department of Educational Psychologyc, University of Minnesota. Research supports that testing with corrective feedback is a viable strategy to improve learning. Common testing feedback mechanisms used in large enrollment general chemistry courses on multiple-choice exams include combinations of the following: scores, bubble response forms with incorrect answers checked, bubble response forms correct answers identified, and/or posting of answer keys with and without explanations. We investigated whether some of these common delayed feedback approaches improved student performance upon repeat testing in general chemistry. In addition, we investigated an additional feedback option whereby an online homework system was used to provide delayed answer-until-correct (AUC) feedback. We hypothesized increased item performance upon retesting with corrective feedback mechanisms as compared to non-corrective feedback. We also hypothesized that reworking the problems until correct for feedback would be superior to reading correct answers. Analysis revealed statistical differences between the 1st and 2nd tests when students were asked to re-answer the items until correct as the delayed corrective feedback mechanism. Statistical differences were not observed between the 1st and 2nd tests with more passive reading of correct answers.

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POSTER 003

Growing functional nanolasers in a large analytical chemistry class

Pamela Doolittlea, Yongping Fua, Vila Rajaratnamb, and Song Jina

Department of Chemistrya, University of Wisconsin Madison; Department of Chemistry and Biochemistryb, University of Wisconsin Milwaukee

Methylammonium lead halide perovskites (CH3NH3PBX3 X=I, Br, Cl), which are the focus of attention for highly efficient solar cells, can be grown as single-crystal nanowires via a simple solution route to make extremely efficient nanolasers. Learning the solution conditions to best grow these materials falls in the realm of analytical chemistry and therefore serves as a wonderful backstory to teach quantitative analysis. We’ve enlisted a large undergraduate analytical chemistry class to help us learn more about how initial solution conditions impact the morphology of products. Students develop methods from the literature to measure concentrations of lead and halides for the reactions. SEM and optical microscopy images analyzed in ImageJ allow students to correlate morphologies to solution conditions. We’ll share our strategies for managing workflow, and meeting learning and research objectives.

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POSTER 004

Investigating Absolute and Relative Scale in an Introductory Anatomy and Physiology Course

Victoria Fisher, Jaclyn Trate, Anja Blecking, Peter Geissinger, and Kristen Murphy

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

The American Association for the Advancement of Science (AAAS) has outlined four

themes that define science literacy; these are systems, models, constancy and change, and scale. More recently, the National Research Council has released the framework for K-12 science education that includes “Scale, Proportion, and Quantity”. Our research has already shown that scale literacy is a better predictor for success in a general chemistry course than traditional measures and integrating scale as a theme in the undergraduate general chemistry curriculum has led to increases in student learning gains as measured by increases in student final exam performance. With the ultimate goal to generalize this research across STEM disciplines, we have begun looking into how students within the biological sciences discipline understand scale and concepts related to scale and how this relates to the understanding of other important biology concepts. One activity, adapted from the work of Gail Jones, and subsequently adapted from a course-wide activity done previously in this research, that targeted these goals was used in a one-on-one interview setting with both novice and experienced students. In this activity students created “bins” to sort objects spanning a wide range of sizes and then given 15 cards containing the names of objects to sort into their bins. After sorting the objects, students were also asked to place the objects on a logarithmic number line. Preliminary data collected from this activity shows that students are less comfortable working with objects and units that fall outside of the visible realm and that students frequently operate within a very narrow range of scale, typically centered around the height of an adult. The complete analysis of this activity and the implications for these findings as they relate to developing scale-themed instruction for the biological sciences will be presented.

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POSTER 005

Uncharacterized Proteins in Pseudomonas brassicacearum

Lamia Tabassum Badhon, Lanlan Han, and Nicholas R. Silvaggi

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Our laboratory recently reported the structural and functional characterization of the enzyme MppP from Streptomyces wadayamensis. We showed that this enzyme is a novel type of pyridoxal-5’-phosphate-dependent enzyme that oxidizes L-arginine to 4-hydroxy-2-ketoarginine in the biosynthetic pathway of L-enduracididine (L-End), a non-proteinogenic amino acid found in the cyclic glycopeptide antibiotic mannopeptimycin. Surprisingly, a BLASTp search of the NR database using the sequence of S. wadayamensis MppP revealed close homologs in a number of species of pseudomonas, including Pseudomonas brassicacearum, P. syringae, P. amygdali, and P. aeruginosa. The gene context of the pseudomonad MppP homologs are different from that of the mannopeptimycin-producing Streptomyces enzyme. In fact, none of the pseudomonad genomes examined contained other key mannopeptimycin biosynthetic genes like the nonribosomal peptide synthetases mppA and mppB, or the other two L-End biosynthetic genes mppQ and mppR. All of this suggests that the pseudomonad MppP homologs are involved in a different pathway. We cloned, expressed, and purified the MppP homolog from Pseudomonas brassicacearum, a soil-dwelling bacterium associated with the roots of plants in the mustard/cabbage family that protects the plants from several bacterial and fungal pathogens. We show here that the P. brassicacearum MppP (PbrMppP) catalyzes the same reaction as the S. wadayamensis enzyme, with nearly identical kinetics. The “MppP operon” in P. brassicacearum consists of a regulatory gene, the MppP homolog, and four additional open reading frames annotated as “hypothetical protein (PbrHYP1),” dihydrodipicolinate synthase family protein (PbrDHPS), “hypothetical protein” (similar to ketoacyl-ACP synthase; PbrKAS), and 2Fe2S-binding protein (similar to ferredoxin, PbrFD), respectively. In order to determine the product of the pseudomonas MppP operon, we have begun cloning and expressing the other enzymes of the pathway. Thus far we have determined the structure of PbrKAS and found that it has a pocket with two histidine and one glutamate residue pointing towards center, which is similar to iron binding site in “UndA fatty acid oxidase”. Future work will focus on identifying the functions of these proteins.

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POSTER 006

Is 2-Hydroxyglutarate an Oncometabolite?

Joseph V. Roman, Khalil I. Ouellette, and Graham R. Moran

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Isocitrate dehydrogenase is a well-known primary metabolism enzyme. Its role in the citric acid cycle is to convert isocitrate into α-ketoglutarate (αKG), while concurrently reducing a molecule of nicotinamide adenine dinucleotide (NAD+) to NADH. In eukaryotes, in addition to its role in primary metabolism this activity is found both in mitochondria and the cytoplasm where it plays a role in secondary metabolism, IDH1. In the cytoplasm IDH1 provides αKG as a substrate to other enzymes such as the Jumonji family of histone demethylases and a key contributor of NADPH to myriad biosynthetic processes. Over the past decade there have been numerous studies of variant forms of IDH1 being associated with several forms of cancer in humans. One notable mutation is the active site arginine 132 to histidine alteration. This change diminishes IDH1’s activity and confers a neomorphic activity, which takes the αKG product and reduces it to 2-hydroxyglutarate (2HG) utilizing the other product, NADPH as a reductant. This variant form is associated with almost 90% of primary gliomas. 2HG is believed to be an oncometabolite that accumulates and inhibits αKG dependent enzymes involved in histone modification, this loss in ability to regulate gene expression is then thought to lead to uncontrolled cell growth. There is a need for a stringent kinetic analysis of ICDH arising from four glaring inconsistencies in the literature, 1.) There are reports of mutations to the cytosolic enzyme that do not confer this neomorphic activity, while still causing cancer. 2.) The R132H mutation seems to be a rather slow enzyme with respect to 2HG formation. 3.) Humans have a 2HG dehydrogensase, whose role in the cell is to take the “oncometabolite”, 2HG, and oxidize it to αKG (apparently nullifying the claimed toxicity). 4.) Furthermore, people with an inactive form of 2HG dehydrogensase suffer from a condition known as 2 hydroxyglutaric aciduria while very infrequently showing any signs of cancer. Our goal is to do a direct comparison of the mechanism by which both the wild type and R132H mutant, and possibly others, do their respective chemistries. In doing so, we hope to lay down a strong mechanism based foundation for understanding the true role of ICDH1 and the metabolic consequences of aberrant activity in diploid organisms.

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POSTER 007

Biochemical Characterization of Atypical Diaphorase Enzymes

Matthew R. Hoag, Joseph V. Roman, Brett A. Beaupre, Nicholas R. Silvaggi, Audrey L. Lamb, and Graham R. Moran

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Human renalase is a flavoenzyme discovered in 2005 that was originally claimed to modulate blood pressure and heart rate by oxidizing catecholamines in blood. This assignment of activity has since been demonstrated to be erroneous, and the enzyme has instead been shown to oxidize toxic isomers of β-NAD(P)H into β-NAD+. Such an important metabolic function would be expected to be present in all kingdoms of life. The structure of human renalase was solved in 2011 by Milani et al., using the pdb coordinates of an unknown oxidoreductase from Pseudomonas syringae as the search model for molecular replacement. We expressed and purified this enzyme and identified its 2- and 6DHNAD(P)-oxidizing activity, making it the first example of a renalase identified in prokaryotes. The Pseudomonas enzyme has proven much more apt to crystallize than the human enzyme, and has afforded high-resolution crystal structures with the reaction product, β-NAD+, and substrate analogues, β-NAD(P)H, bound in the active site. Stopped-flow experiments studying the reductive half-reactions of each enzyme have revealed distinct specificity profiles. To the bacterial enzyme, the NADH isomers bind more tightly than NADPH isomers, and the 2DH isomers reduce the active-site flavin more rapidly than the 6DH isomers. For the human enzyme, similar specifies among all four substrates were measured. Old yellow enzyme is another diaphorase enzyme that is being investigated in our lab. The first flavoprotein to be discovered (Warburg & Christian, 1934), it has puzzled researchers for decades with regard to its metabolic role. While it has been shown to rapidly oxidize β-NADPH, it has shown remarkable promiscuity with regard to the oxidative half-reaction. Our lab has hypothesized that this enzyme may exist to preserve the functioning of glycolysis and the pentose phosphate pathways under anaerobiosis, by oxidizing β-NADH and β-NADPH using a variety of endogenous electron acceptors. Our lab recently characterized two forms of the enzyme from Saccharomyces cerevisiae, finding similar specificities for both NADH and NADPH consistent with this hypothesis. We have also begun metabolomics studies on cerevisiae knockout mutants using LC/MS, in order to identify any endogenous substrates of the OYE oxidative half reaction.

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POSTER 008

Understanding proteome dependent cellular zinc trafficking to form native Zn-proteins

Afsana A. Mahim and David H. Petering

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Trafficking pathways from the entry of Zn2+ into cells to the formation of native Zn-proteins are poorly understood. Studies have reported that Zn2+ binds to apo-metallothionein to make Zn-metallothionein (Zn-MT), which then transfers Zn2+ to apo-proteins. However, other studies revealed the existence of a substantial concentration of adventitious protein binding sites for Zn2+, suggesting that the proteome may compete for binding intracellular Zn2+ or act as an intermediate along the pathway to constitute Zn-proteins. The present study focuses on the hypothesis that proteomic binding sites for Zn2+ are involved in the trafficking mechanism related to the formation of native Zn-proteins. The Zn2+ buffering capacity of proteome was monitored with a weak Zn2+ sensor, Zincon (ZI). The Zn2+ titration of proteome (LLC-PK1 cells) shows that

initially ZI (Kd ~ 10-5 M) could not compete for added Zn2+ with the proteome’s higher affinity Zn2+

binding sites (4x concentration of native Zn-proteins). This region of the titration was followed by an increase in absorbance previously thought to represent free Zn-ZI. However, the absorbance spectrum of Zn-ZI was red shifted. This part of the titration revealed the presence of lower affinity binding sites for Zn2+ (about 15x concentration of native in the proteome Zn-proteins). Fractionation of the resultant reaction mixture showed that absorbance due to Zn-ZI was confined to the proteome, suggesting the formation of ternary adducts, proteome-Zn-ZI. Also, preincubation of the proteome with N-ethyl-maleimide (NEM) abolished the first phase of the titration. Therefore, proteome contains large concentration of both high and low affinity, nonspecific binding sites that compete with ZI for Zn2+ or participate in ternary complex formation. To further investigate the role of proteome in Zn2+ trafficking, Zn-MT was induced in LLC-PK1 cells by exposure to Zn2+. Besides binding to MT, added Zn2+ also nonspecifically associated with proteome, showing that proteome binding sites have high affinity for Zn2+. When apo-carbonic anhydrase (apo-CA) was titrated with Zn2+ in the presence of proteome, the formation of Zn-CA occurred but was depressed. These results show that proteomic non-specific zinc binding sites can play a significant role in Zn2+ trafficking to form native Zn-proteins and possibly in Zn2+ signaling. Supported by NIH grant ES-024509 and RGI award from the University of Wisconsin-Milwaukee.

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POSTER 009

Substituent Effects on Photo -Induced DNA Interstrand Cross-Link Formation Via Carbocation

Heli Fan and Xiaohua Peng

Department of Chemistry and Biochemistry and the Milwaukee Institute for Drug Discovery,

University of Wisconsin-Milwaukee

Several bifunctional compounds with various aromatic substitutions and benzylic leaving groups were synthesized. Time dependent ICL formation and ICL yield measurement were used for reactivity and ICL efficiency study. Radical or carbocation trapping both during ICL formation and monomer trapping process was used for the mechanism investigating. Heating stability study for the ICL products was used for the cross-linking sites study. Substitutions and leaving groups affect the reactivity and ICL efficiency. The ICL formation was undergo carbocation mechanism which was generated either through radical or via direct heterolysis. Purines were the major cross-linking sites for most compounds.

22

POSTER 010

Effects of Polymers on Viscosity and Adhesion of Silica to Nonporous Surfaces

Aimee Maher and Jennifer E. Mihalick

Departments of Art and Chemistry, University of Wisconsin-Oshkosh Painting on glass with glass as an artistic expression, in terms of using a room-temperature process like a silica sol-gel, is an uncommon application. The sol-gel process makes the high heat of a foundry obsolete in the creation of a glass monolith from a liquid. In this process, colloidal particles polymerize into a semi-solid that will continue to decrease in volume and increase in density as surplus liquids precipitate out of the sol-gel. The product of this irreversible gel process is a porous silica glass. Colorants, such as metal salts, dyes, and other organic materials, can be added to a sol-gel in varying concentrations. Viscosity and adhesion to non-porous surfaces can be impacted by using a hybrid PVA solution.

23

POSTER 011

Zn- and Cd-protein Identification in Proteomic Samples Using LA-ICP-MS: Current Status

Eric D. Lund and David H. Petering

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Zinc is an essential metal in all life forms. From protein folding and stability to enzyme catalysis, zinc is used widely to support many functions inside the cell. The collection of proteins within the cell commonly known as the proteome contains as many as 2800 Zn-Proteins. Most of these Zinc proteins have not been characterized. The problem are the missing methods to isolate and identify Zn-proteins at the proteomic level. Potentially, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) can act as a powerful and sensitive microanalytical technique to detect metals bound to proteins. Native methods for the determination of such proteins has been lacking until recently. The new method of native sodium dodecyl sulfate polyacrylamide gel electrophoresis (NSDS-PAGE) solves this issue. The present work involves LA-ICP-MS of Zn- and Cd- proteins from LLC-PK1 cell supernatant that have been separated by DEAE-HPLC and then NSDS-PAGE. Repetitions of LA-ICP-MS resolve reproducible patterns of peaks of Zn and Cd qualitatively but not quantitatively. Proteins can be visualized by silver staining and correlated to corresponding gel runs. Special Zn-protein staining is under investigation. Pre-electrophoresis with EDTA reduces the background level of Zn in the gel. Isotopically labelling cells with 70Zn to distinguish 70Zn-proteins from background spikes fails due to in-gel exchange with adventitious Zn. Proteomic Zn also exchanges with Cd2+. Exchange has been confirmed at the individual protein level with many bands of Zn in control lanes transformed into bands containing both metals in proteome samples exposed to Cd. Some have lost all of their Zn. Problems and prospects for this methodology will be described. Supported by NIH grant ES-024509 and a Research Growth Initiative grant from the University of Wisconsin-Milwaukee.

24

POSTER 012

Integration of Scale-Themed Instruction Across the General Chemistry Curriculum

Jaclyn Trate, Anja Blecking, Peter Geissinger, and Kristen Murphy

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

In 1982, in response to a growing demand for a scientifically literacy population, two

organizations, the AAAS and NCISE published reports that proposed using themes to bridge scientific disciplines. The NCISE report identified “9 explanatory concepts” which included organization, cause and effect, systems, scale, models, change, structure or function, discontinuous and continuous properties, and diversity. The AAAS report, as part of Project 2061, identified 4 themes that define science literacy which included systems, models, constancy and change, and scale. In 1993, the AAAS released the Benchmarks for Science Literacy which outlined what all students should know or be able to do related to each common theme by the end of grades 2, 5, 8, and 12. However, prior to the release of the Framework for K-12 Science Education in 2012, and subsequent release of the Next Generation Science Standards in 2013, scale was not included in any national science education standards. Now incorporated as one of seven crosscutting concepts, “scale, proportion, and quantity”, little is known regarding the degree to which scale is incorporated into instruction.

In disciplines like chemistry, undergraduate students are routinely confronted with concepts of scale and consistently demonstrate underdeveloped skills in understanding and applying concepts of scale. Previous research in this field led to the development of two assessments, the Scale Literacy Skills Test and Scale Concept Inventory, for measuring student scale literacy. Using these assessments, scale literacy was found to better predict student success in general chemistry than other traditional predictors of student success such as ACT and placement test scores. Expanding upon the work of Gerlach and co-workers, the work described here outlines the development and systematic integration of a scale-themed curriculum in both general chemistry I and II courses. Throughout 10 semesters of testing, supplemental instruction, laboratory experiments, and lecture instructional materials were developed and adapted to feature explicit themes of scale and implemented into both courses. When all three instructional methodologies are simultaneously administered, consistent positive conceptual learning gains are observed over repeated semesters of testing.

25

POSTER 013

Unnatural Amino Acid Containing Mucin Mimotope-Antibody Binding Study by STD NMR

Lysengkeng Her and Thao Yang

Department of Chemistry, University of Wisconsin-Eau Claire

The binding epitope GVTSAPDTRP, found within the tandem-repeat domain of MUC1 mucin, is recognized by the immune system and binds mucin monoclonal antibody SM3. This study concerns the synthesis, characterization and antibody binding of a MUC1 mimotope of the upstream epitope GVTSAPD that the P6 residue has been substituted by an unnatural amino acid isonipecotic acid. Peptide-Antibody binding study was carried out by the Saturation Transfer Difference (STD) NMR technique. The STD NMR results obtained indicate that the binding between the mimotope and the antibody occurred mainly at the piperidyl ring and at the CH3- groups of V2, T3 and A5 residues. The synthesized peptide sample contained an isomer that could not be separated by usual means. The mimotope-antibody binding suggests that only one of the isomer peptide binds the antibody. Supported by NSF REU grant award #1460728 to the University of Wisconsin-Eau Claire, summer 2017.

26

POSTER 014

FOSTERING COMMUNITY APPRECIATION OF SCIENCE IN AND OUT OF THE CLASSROOM

Bassam Z. Shakhashiri

Wisconsin Initiative for Science Literacy, Department of Chemistry, University of Wisconsin-

Madison

The University of Wisconsin System chemistry faculty and staff are scientists-citizens entrusted with advancing chemistry and communicating chemistry. We aim to educate future scientists and to achieve science literacy among all students, institutional colleagues, and employees. Science literacy enlightens and enables people to make informed choices, to be skeptical, and to reject shams, unproven conjecture, and to avoid being bamboozled into making foolish decisions where matters of science and technology are concerned. Science literacy is for everyone—scientists, artists, humanists, all professionals, the general public, youth and adults alike. We must continue to improve connectivity between science and society. I lead an integrated, comprehensive approach aimed at reaching audiences in communities throughout Wisconsin and neighboring states. In keeping with the best traditions of the Wisconsin Idea, one major goal is to improve the connectivity between the UW System and the citizens of Wisconsin and neighboring states. Another goal is to foster collaboration between various communities and the UW System to better serve mutual needs. Listening to youth and adults in schools and community settings and improving the quality of education in urban and rural settings requires deliberate, deep involvement by all stakeholders. Providing opportunities to enhance the quality of learning and nurturing personal growth of students is crucial to fulfilling human potential. Professional development of faculty teachers and inclusion of families in meaningful experiences greatly contribute to creating a sustainable environment for the betterment of society. Those interested in joining my efforts, please visit www.scifun.org. Contact us at scifun@chem.wisc.edu.

27

POSTER 015

Automated Patch Clamp to Determine the Pharmacology and GABAA receptor Subtype Selectivity of Imidazobenzodiazepines

Amanda N. Nieman, Gloria S. Forkuo, Nina Y. Yuan, Ted W. Harris, Md. S. R. Roni, Rajwana Jahan, Guanguan Li, Michael Rajesh Stephen, Douglas A. Steeber, Douglas C. Stafford, James M.

Cook, and Leggy A. Arnold

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee Asthma is characterized by increased mucus hyperplasia, airway hyperresponsiveness, and inflammation mediated leukocytes including CD4+ T-cells.1 CD4+ T-lymphocytes express distinct GABAA receptor (GABAAR) subtypes, which are ligand-gated pentameric chloride channels that bind benzodiazepines and other compounds.2 We observed a reduction of CD4+ T-cell counts in mice with lung infection after treatment with imidazobenzodiazepines. As a result, we show that these compounds can induce CD4+ T-cell transmembrane current changes in the presence of GABA, the endogenous ligand of the GABAARs. The pharmacological effect was determined using the IonFlux16, which adapts the normally tedious and time-consuming patch clamp assay through automation for high throughput screening. The pharmacological effects of imidazobenzodiazepines is governed by their ability to interact with different α-subunit containing GABAARs. Therefore, we established mammalian cell lines that express α1-6β3γ2 GABAARs. The characterization of cells expressing α1β3γ2 and α4β3γ2 GABAAR will be presented. 1Long, A. 2005. Addressing unmet needs in asthma care. In Kaliner, M.A. (ed), Asthma. P&T Digest. 30(11):16-22. 2Mendu, S. K., Bhandage, A., Jin, Z., & Birnir, B. (2012). Different Subtypes of GABA-A Receptors Are Expressed in Human, Mouse and Rat T Lymphocytes. PLoS ONE, 7(8), e42959

28

POSTER 016

Deciphering the Function of NTF2-like Proteins Associated with Polyketide Biosynthesis in Actinomycetes

Nemanja Vuksanovic1 , Xuechen Zhu2 , Charles E. Melançon III2, and Nicholas R. Silvaggi1

1Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

2Department of Chemistry and Chemical Biology, University of New Mexico Bioactive natural products are the active components in many therapeutic drugs, and as antibiotic resistance emerges as a global threat, a quest for novel natural products with antibiotic activity becomes increasingly important. Actinomycetes are known to produce many bioactive secondary metabolites, such as polyketides, some of which are powerful antibacterial agents. The gene cluster involved in production of actinorhodin, a benzoisochromanequinone antibiotic found in Streptomyces coelicolor A3(2), has been extensively studied. However, the role of the ActVI-ORFA gene has remained controversial. Strains where the gene has been disrupted still produce actinorhodin, only at muchreduced levels compared to the wild-type strain. A number of studies have suggested a regulatory role for ActVI-ORFA. Our in vivo kinetic studies of S. Coelicolor mutants show that strains without functional ActVI-ORFA accumulate at least one off-pathway intermediate. We also report the structures of ActVIORFA and a homologue, CA-Cyc3, from Catenulispora acidiphila. Both proteins exhibit the α-and-β barrel fold characteristic of NTF2-like superfamily proteins and possess a His/Asp diad, a motif found in a variety of enzymes that perform acid/base catalysis. Taken together, our functional and structural data suggest that ActVI-ORFA may have an enzymatic role, directing the pathway away from the shunt product and toward the synthesis of actinorhodin.

29

POSTER 017

Antibiotic and Antioxidant Activities of Herbal Preparations

Adrianne Bunn, Miranda Rang, Wyatt Cook, Brandon Kaufman, and Lisa Kroutil

Department of Chemistry and Biotechnology, University of Wisconsin-River Falls Herbal medicines have been used to treat numerous common illnesses throughout recorded history, but they fell out of favor with the development of modern pharmaceuticals. These remedies have seen a recent resurgence in popularity. Much folk lore, sometimes conflicting, surrounds how to prepare herbal remedies. This highlights the need to examine such claims in order to determine their validity and to determine optimal formulation methods for specific uses. We initiated studies with Artemisia vulgaris (mugwort) investigating its antibacterial, antioxidant, and radical scavenging properties, as an extension of literature reports on other members of the genus. We also decided to screen a number of available herbal tinctures. These preliminary studies have shed some light on considerations for preparing ethanolic tinctures of mugwort for their antibacterial and antioxidant properties. They have also identified additional promising herbal remedies for further research. HPLC analysis has begun for the identification of active components.

30

POSTER 018

Investigation of the Biological Function of Calcitroic Acid and Its Phase Two Metabolites

Olivia Yu, Elliot DiMilo, Viktoriia Senych, Kelly A. Teske, and Leggy A. Arnold

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Calcitroic acid was isolated and characterized more than four decades ago. These experiments used radioactive 1,25-dihydroxyvitamin D3 to enable the identification of radioactive calcitroic acid formed in vivo, which was subsequently identified by using extraction and derivatization. Calcitroic acid was found to be predominantly formed in the liver and secreted into the gut via the bile duct (enterohepatic circulation) leading to higher concentrations in the intestine. The enzyme responsible for the formation of calcitroic acid is CYP24A1, which is predominantly expressed in the liver and kidney; however, recent research has shown that this enzyme is also expressed in many other cells types. Herein we describe the synthesis of calcitroic acid. We demonstrated that calcitroic acid can bind the vitamin D receptor (VDR) using a fluorescence polarization-based binding study. Other findings include the calcitroic acid-induced gene regulation of p450 enzymes in colon and prostate cancer cells. We hypothesize that calcitroic acid is a regulatory ligand for VDR especially in the intestine in order to facilitate the metabolism of irritants by p450 enzymes.

31

POSTER 019

Localization of novel imidobenzodiazepines in asthmatic mouse lung tissue by MALDI imaging mass spectrometry

Margaret L. Guthrie, Nicolas Zahn, Guanguan Li, Revathi Kodali, Glorio Forkuo, Morgan E.

Stevenson, Shama Mirza, James M. Cook, Douglas C. Stafford, and Leggy A. Arnold

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Asthma is a prevalent inflammatory disease of the airways with unmet needs for improved treatment strategies. Imidobenzodiazepines have been shown to reduce inflammation and airway hyperresponsiveness through a novel mechanism of action: targeting the GABAA receptor. Imaging mass spectrometry (IMS) provides the ability to image tissue, organ, or even whole body sections by employing matrix assisted laser desorption ionization (MALDI) technology. MALDI-IMS provides the capability to generate an ion density based image for the drug compound mass-to-charge ratio (m/z) that serves as a ‘heat map’ to locate where in that particular tissue the drug is located. Mice were dosed orally with novel benzodiazepines that have been shown previously to be alpha-5 selective GABAA receptor modulators. Tandem mass spectrometry was employed using a MALDI-TOF/TOF instrument to identify the drug compounds in lung tissue sections cut at 20 µm thickness. The mass spectrum image was then compared with histological stains to further illustrate the distribution morphologically.

32

POSTER 020

Synthesis of novel compounds to study expression and localization of the vitamin D receptor

Tania Mutchie, Rodrigo Ramos Morales, Fernando Ramos Morales, and Leggy A. Arnold

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

The vitamin D receptor (VDR) is a nuclear hormone receptor that regulates cell

proliferation, cell differentiation, and calcium homeostasis. VDR is a transcription factor that can be found in the cytosol and the nuclei. Upon binding to 1,25-dihydroxyvitamin D3 (calcitriol) and other vitamin D active metabolites, VDR localizes in the nuclei where it interacts with coregulators and DNA. Universal tool compounds to further elucidate the expression and localization of VDR are not available; therefore, we are developing new VDR ligands that can easily be traced in vitro and in vivo. The partial synthesis of these ligands is presented.

33

POSTER 021

Novel Deuterated GABAAR-a6 Subtype Selective Ligands with Improved Metabolic Stability and Enhanced Bioavailability: Targeting Trigeminal Orofacial

Pain, Neuropsychiatric Disorders, and Depression

Daniel E. Knutsona, Revathi Kodalia, Michael R. Stephena, Nicolas M. Zahna, Leggy A. Arnolda, James M. Cooka, Vladimir Dobricicb, Miroslav M. Savićb, Marko D. Mihovilovicc, Marco Trevenc,

Margot Ernstc , and Werner Sieghartc

a Department of Chemistry & Biochemistry, Milwaukee Institute for Drug Discovery, Univ. of Wisconsin-Milwaukee

b Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia c Center for Brain Research, Department of Molecular Neurosciences, Medical University

Vienna, Vienna, Austria GABAA receptors (GABAAR) are the major inhibitory neurotransmitter receptors in the mamma-lian brain and the target of many clinically important drugs which act at GABAAR binding sites.

GABAAR containing the subtype are primarily expressed in the granule cells of the cerebellum, as well as in the cochlear nucleus and olfactory bulb. Lower expression has recently been re-

ported also for the hippocampus. Recent publications suggest that receptors may play a role in trigeminal orofacial pain, neuropsychiatric disorders with sensori-motor gating deficits (such as tic disorders, certain symptoms of schizophrenia, obsessive compulsive disorder and atten-

tion deficit disorders), and depression. However, the function of -containing GABAAR in brain physiology is still largely unclear. Consequently, designing compounds selective for the GABAAR

subtype would greatly assist in the determination of which physiological processes they me-

diate. Recently, the GABAAR binding site was discovered6 and pyrazoloquinolinones such

as PZ-II-029 were reported as the first GABAAR subtype functionally selective ligands to date. However, the bioavailability and the half-life of these ligands was of concern due to the possible O-demethylation in vivo as well as their limited solubility in water. To increase the bioavailability of the active ligands, we utilized the primary deuterium kinetic isotope effect (DIE) by selective replacement of H atoms for D atoms at key metabolic positions (methoxy groups) on the ligands. In addition, water solubility of the ligands was improved by N-hetero substitution into the “A-ring” or “D-ring”. Here we report the synthesis of the first deuterated GABAAR-a6 subtype functionally selective ligands and demonstrate that the OCD3 substituted ligands, such as DK-I-56-1, exhibited retained

efficacy for the GABAAR subtype and improved metabolic stability as well as increased brain concentrations (Cmax) with longer half-lives (t1/2) over the parent OCH3 counterparts.

34

POSTER 022

Engineering Glyoxylate Reductase’s Active Site

Brandon Patterson, Nemanja Vuksanovic, and Nicholas R. Silvaggi

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee

Emerging antibiotic resistance represents a global threat and a quest for novel antibiotics is becoming of increasing importance. Our work is focused on elucidating structural and enzymatic properties of proteins involved in the biosynthetic pathway of a non-proteinogenic amino acid, L-enduracididine, which is a component of a number of antibiotics such as teixobactin and mannopeptimycin (MPP). These peptides demonstrate potent activity against methycilin-resistant Streptomyces Aureus (MRSA). We are currently studying three enzymes in MPP biosynthetic clusters; MppP, MppR, and MppQ. Our preliminary data indicates that MppQ possesses transaminase activity towards 2-keto argininine, and to obtain accurate kinetic parameters we coupled this reaction with E.Coli Glyoxylate reductase (GR). However, we discovered that Glyoxylate reductase surprisingly has a higher affinity towards 2-keto arginine over its native ligand, glyoxylate. Our structural studies have confirmed that 2-keto arginine is indeed a substrate for GR. This work shows steady state kinetic data for Glyoxylate reductase and 2-keto arginine, Glyoxylate, and NADPH. We have found that there is significant substrate inhibition when reaction was conducted with Glyoxylate, but not with 2-keto arginine. This suggests that there could be separate binding sites for the substrates, as well as two different reaction mechanisms. Understanding the differences in binding between these substrates would allow us to modify the active site and design an enzyme that exclusively reacts with Glyoxylate to be used in our studies of MppQ.

35

POSTER 023

Synthesis of Novel Imidazobenzodiazepine Oxazole Bioisosteres as Potential Alpha 2, 3 Subtype Selective GABA(A) Receptors Agonists with Excellent

Metabolic Stability, Pharmacokinetics, and Anxiolytic Efficacy

Guanguan Li1, Kashi R. Methuku1, Michael M. Poe1, Jeffrey M. Witkin2, Jeffrey M. Schkeryantz2, Margot Ernst3, and James M. Cook1

1 Department of Chemistry & Biochemistry and Milwaukee Institute Drug Discovery, University of Wisconsin-Milwaukee

2 The Lilly Research Labs, Eli Lilly and Company, Indianapolis, IN 42685 3 Department for Molecular Neurosciences, Medical University of Vienna, Vienna, Austria

Benzodiazepines (BZD) are commonly used in the treatment of mental disorders such as anxiety disorders, schizophrenia, depression and neuropathic pain which affect over 18% of the US population. However, many patients respond poorly to the classical BZD’s by suffering a variety of adverse effects including sedation, ataxia, tolerance, and addiction. Hz-166 (Zeilhofer et al.), a previously characterized 1,4-Benzodiazepine was identified as an αlpha 2/αlpha 3 GABA(A)R subtype-selective ligand (Rivas et al.), with significantly reduced ataxia/sedative effect, but with anticonvulsant, anxiolytic and anti-nociceptive properties. The ester moiety in Hz-166 was quickly metabolized in rodents to its corresponding carboxylic acid, which resulted to a suboptimal pharmacokinetics and precluded further study of it in rodents. A series of new heterocyclic bioisosteres at C-3 with various substituents were then designed, synthesized, and evaluated to improve brain penetrability and increase plasma and brain exposure. A novel lead compound, the 1,3-oxazole KRM-II-81 (Poe et al., J. Med. Chem., 2016) was the most promising candidate of the bioisostere series as a potent, αlpha1 sparing GABA(A)R ligand with excellent PK, and drug stability in rodent anxiety models. In addition, KRM-II-81 exhibited increased anticonvulsant potency and efficacy in rodent seizure-induction models. Moreover, it exhibited the anxiolytic-like effects in a Vogel conflict test and a mouse marble-burying assay compared to the parent Hz-166, with no sedative effects. Thus, KRM-II-81 represents a significant advance in the pharmacology of these anxiolytic and anticonvulsant imidazobenzodiazepines. This work was supported by NIH grants, NS076517 and MH096463.

36

POSTER 024

Optical Methods for Detecting Heavy Metals in Aqueous Applications

Joseph T. Labeots, Paul E. Henning, and Joseph H. Aldstadt

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee Heavy metals that are discarded into the environment may pose severe threats to public health, and are thus heavily regulated. Presently, there is no reliable, real-time monitoring methods for heavy metals in water. This lack of feedback for industrial applications often leads to wasted resources due to massive overtreatment to avoid costly penalties. Current methods involve sample collection and subsequent off-site analysis, which can take several days. This does not allow for quick feedback should metal ion fluctuations occur in the treatment process. Optical detection of metals is a viable option assuming a suitable sensor molecule is available as seemingly conflicting requirements may occur. A sensor molecule that is highly specific for one metal with a strong binding affinity can handcuff the ability to measure multiple metals continuously. Thus, one must be careful when trying to obtain highly specific sensors. Two methods have been used to approach this problem. First, a method that can overcome this restriction is the use of an array of sensors that respond uniquely to a set of distinct metals. These novel sensors exhibit a spectral change in their absorbance when metal ions are present. A multivariate approach can be used to build a predictive model that depends on which metals exist in the solution. This model can then simultaneously predict the concentrations of multiple metals in an aqueous sample with exquisite accuracy. The second method that was investigated was fluorescence lifetime based measurements. This was done with the use of a commercially available fluorescent sensor. The fluorescence lifetime is an intensity-independent quantity and does not require periodic recalibration, which is a key benefit for optical sensing. The sensor tested exhibits steady-state fluorescence quenching by many metals, but also exhibits a unique “lifetime-based selectivity” for copper and cobalt.

37

POSTER 025

Histone Deacetylase (HDAC) Inhibitors as Anti-Cancer Agents

Mizzanoor Rahamana, Damon Hinza, Sreya Biswasa, Doug Steeberb, and M. Mahmun Hossaina

Departments of Chemistry & Biochemistrya and Life Sciencesb, University of Wisconsin-

Milwaukee Natural product-based histone deacetylase (HDAC) inhibitors such as vorinostat (SAHA), thailandepsin and romedepsin (FK228) are usually very potent, moderately isoform-selective, but are often associated with poor solubility, ineffective against solid tumors and excessive cytotoxicity. We then designed and synthesized a series of compound fragments based on the scaffolds of thailandepsins A and B, and FK228 from molecular modeling. In general, all of the fragments had significantly improved solubility and much less cytotoxicity compared to the parent compounds and retained potent HDAC inhibitory activity towards class I HDACs, particularly HDAC1. These structure–activity studies provide new chemical entities for research exploration and new knowledge for the design of more desirable HDAC inhibitors. This synthetic protocol provides a convenient approach to many new HDAC inhibitor analogues with a TDP-A scaffold.

38

POSTER 026

Evaluating Student Motivation in Organic Chemistry with Flipped-Peer-Led Team Learning Approach

Yujuan Liua, Jeffrey R. Rakerbc, and Jennifer E. Lewisbc

aDepartment of Chemistry, University of Wisconsin-Parkside

bDepartment of Chemistry and cCenter for Improvement of Teaching and Research on Undergraduate STEM, University of South Florida

The chemistry-specific instrument, Academic Motivation Scale-Chemistry (AMSChemistry), was based on Self-Determination Theory. AMS-Chemistry was used to evaluate the integration of two active learning pedagogical approaches in the same organic chemistry course. Data were gathered with AMS-Chemistry in two organic chemistry courses taught by the same instructor, one was lecture based and the other integrated flipped classroom and peer-led team learning (PLTL). Descriptive statistics showed that students in both courses were more extrinsically motivated and their motivation moved in negative directions across a semester. Factorial multivariate analysis of covariance revealed a main effect of pedagogical approach. Students in the Flip-PLTL environment were more motivated toward chemistry at the end of the semester while controlling for the motivation pre-test scores. Correlation results revealed variable relationships between motivation subscales and academic achievement at different time points. In general, intrinsic motivation subscales were significantly and positively correlated with student academic achievement; Amotivation was negatively correlated with academic achievement. The results provided evidence in support of AMS-Chemistry score interpretation in both lecture-based and active learning environments. The findings suggest that Flip-PLTL pedagogies are important in improving student motivation toward chemistry.

39

POSTER 027

General Strategy for the Total Synthesis of C-19 Methyl Substituted Sarpagine/Macroline Indole Alkaloids

Md Toufiqur Rahman†, Jeffrey R. Deschamps‡, and James M. Cook†

†Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee ‡Center for Biomolecular Science and Engineering, Naval Research Laboratory

Sarpagine/macroline/ajmaline is a large group of biosynthetically related indole alkaloids. These alkaloids occur mainly in the Apocynaceae family of plants and different species of Alstonia, Rauwolfia, and Strychnos. To date, many alkaloids, both monomeric indole and dimeric bisindole alkaloids with potential biological activities have been isolated. The C-19 methyl substituted macroline/sargapine/ajmaline-type alkaloids are an emerging subgroup of alkaloids featuring a methyl function at the C-19 position of the core structure. Currently, more than thirty (30) alkaloids belong to this group. The alkaloids have been isolated from different species of Alstonia, Rauwolfia, and Vinca medicinal plants, which have been used in traditional and folk medicines in many countries for centuries. The majority of these alkaloids have never been evaluated for their bioactivities presumably, due to the paucity of isolated materials. Recently, alkaloids from this group have been reported to exhibit potential biological activities such as NF-κB inhibition (i.e. anticancer, N(4)-methyltalpinine), potent leishmanicidal (Nb-methyl, Nb-21-secotalpinine), anti-plasmodial (talcarpine), and ganglionic blocking (verticillatine). Consequently, developing a general strategy for the total synthesis of any member of this subgroup is of great importance. This general and practical approach would allow one to synthesize these alkaloids in required amount for biological evaluation and structure-activity relationship (SAR). Besides, the enantiomers of the alkaloids would be accessible following the same route. The asymmetric Pictet-Spengler (P. S.) reaction has been one of the most versatile reactions in organic synthesis. To date, numerous improvements and applications of this reaction have been achieved. Furthermore, the asymmetric version of this reaction has been utilized in the total synthesis of many sarpagine-related indole alkaloids, as well as in many other scaffolds. In our latest endeavor, we have developed a shorter and improved route to the key tetracyclic core-structure towards C-19 methyl substituted sarpagine/macroline-type indole alkaloids. This novel approach entails an entirely new asymmetric Pictet-Spengler reaction with internal asymmetric induction. This new asymmetric P. S. reaction allowed us to access the key intermediates either from L- or D-tryptophan. Besides, a copper-mediated enolate-driven cross-coupling reaction has been executed as a successful alternative to the previously applied palladium-catalyzed cross-coupling process. This ultimately resulted in the first total synthesis of more than eight C-19 methyl-substituted sarpagine/macroline-type indole alkaloids.

40

POSTER 028

Development of a Solid-Phase Microextraction-Gas Chromatography Method for the Maillard Reaction

L. Heidenreich and J. Aldstadt

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

The Maillard Reaction involves a series of pathways by which sugars and amino acids condense. Although the Maillard Reaction has implications in food processing, environmental chemistry, and human physiology, intermediate mechanisms and kinetics are not well understood. Reaction factors such as pH, temperature, and ionic strength result in a wide variety of products. The initial stages of the reaction of a simple sugar (methyl glyoxal) and amino acid (phenylalanine methyl ester) were studied. Solid-Phase Microextraction (SPME) with gas chromatography (GC) was employed to determine the complexity of the reaction mixtures and to quantify changes in concentrations over time. Optimization of the SPME GC method, including derivatization of the reaction product, will be described.

41

POSTER 029

Cloning of Hydroxylamine Oxidoreductase from Nitrosomonas europaea into Shewanella oneidensis

David Koltermann and A. Andrew Pacheco

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Hydroxylamine Oxidoreductase (henceforth HAO) is a trimeric protein found to contain 7 c-type hemes and one unique heme called P-460 for every monomer of the protein. HAO is a necessary part of the bacterium Nitrosomonas europaea’s ability to participate in the nitrogen cycle. The function of HAO in N. europaea is to catalyze the oxidation of hydroxylamine to nitrite. The primary goal of this project was to clone the HAO gene into the bacterium Shewenella oneidensis. Whereas N. europaea cultures take many weeks to grow a minimum usable optical density (OD), S. oneidensis cultures grow to a much higher OD overnight. This in turn means that substantially higher quantities of HAO can be purified from S. oneidensis than from N. europaea cultures, and they can be obtained more rapidly. This is an enormous advantage for experiments performed in the Pacheco group, which require large quantities of HAO. An important reason why S. oneidensis cells in particular were chosen is for their ability to readily produce c-type hemes. This poster will present the progress made to date in the attempt to clone HAO into S. oneidensis.

42

POSTER 030

Cytochrome c nitrite reductase (ccNiR)-catalyzed reduction of nitrite to nitric oxide by ferrocyanide: insights into the mechanism of ccNiR-catalyzed

ammonification

Mahbbat Ali and A. Andrew Pacheco

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee Cytochrome c Nitrite Reductase (ccNiR) is a periplasmic, decaheme homodimeric enzyme that catalyzes the six-electron reduction of nitrite to ammonia (ammonification). Under physiological conditions ccNiR catalyzes the process without release of intermediates. However, in vitro we have found it possible to trap putative intermediates, or to release partially reduced nitrogen species such as nitric oxide, by controlling the electrochemical potential at which reduction takes place. Such experiments provide valuable insights regarding ccNiR-catalyzed ammonification of nitrite. When the weak reductant ferrocyanide is used as the electron source, S. oneidensis ccNiR catalyzes the one-electron reduction of nitrite to nitric oxide. The reaction rate has hyperbolic dependence on nitrite and ferrocyanide concentrations, and linear dependence on ccNiR concentration. Kinetic studies also show that the rate of NO production is pH-dependent, and that an amino acid with pKa of 6.9, probably His268, needs to be protonated for the enzyme to be active. The mechanistic implications of these findings will be discussed in the context of earlier investigations of ccNiR.

43

POSTER 031

Monitoring Metal Pollutants in Waste Water

Sarah A. Oehm, Tyler Fenske, Trevor Hagemann, Joe Labeots, Paul Henning, Peter Geissinger, and Alan W. Schwabacher

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Current technology in wastewater treatment consists of the overtreatment of water with precipitation agents that adsorb metal and generate solid waste and may take several weeks to verify that the effluent water has met environmental standards. Industrial generators of wastewater must send samples to a third party lab, off-site, and wait for a return analysis of their wastewater quality. Our research seeks to mitigate the costs of resources and time to help companies comply with industrial regulation, while maintaining their economic bottom line and ensuring that spikes in pollution are treated appropriately before discharging to our fresh water systems. We have developed technology using absorbance dye probes that when bound covalently to a solid support can be used as an array of sensors in a flow cell to monitor metal ion concentration in real time.

44

POSTER 032

Microsomal stability determination of novel Imidazobenzodiazepines

Md Shadiqur Rashid Roni and Leggy A. Arnold

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

The microsomal stability of potential drug candidates plays a very important role during drug development. The in vitro stability is determined in the presence of liver microsomes that contain P450 enzymes responsible for metabolism of approximately 60% of all marketed drugs. The enzymatic reaction is followed in time by taking aliquots at various time points and the amount of the parent compound is quantified to determine important properties such as metabolic rate and half-life. The analysis is carried out with the help of a triple quadrupole equipped LC and requires quantitative method development and optimization for each drug. Simplified steps include: 1) Detection of drug molecule; 2) Identification of product ions; 3) Determination of a suitable internal standard (ISTD); 4) Identification of precursor and product ions for the ISTD; 5) Chromatographical baseline separation of drug and ISTD; 6) Determination of response ratio of drug and ISTD at different time points to calculate the metabolic rate and half-life. We will present the microsomal stability of several new imidazobenzodiazepines using this method, which were generated at the Shimadzu Laboratory for Advanced and Applied Analytical Chemistry using a Shimadzu 8040 LCMS/MS.

45

POSTER 033

Synthesis of New Benzodiazepines that Function as α5-GABAA Receptor Ligands to Target Group 3 Medulloblastomas

Farjana Rashid, Taukir Ahmed, Kashi Reddy Methuku, Oliver Jonas, Soma Sengupta, and James

M. Cook

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee Medulloblastoma is the most common childhood malignant brain tumor. The most lethal medulloblastoma subtype exhibits a high expression of the GABAA receptor α5 subunit gene and MYC amplification. New benzodiazepines have been synthesized to function as α5-GABAA receptor ligands. To compare their efficacy with that of standard-of-care treatments, we have employed a newly developed microscale implantable device that allows for high-throughput localized intratumor drug delivery and efficacy testing. Microdoses of each drug were delivered into small distinct regions of tumors, as confirmed by tissue mass spectrometry, and the local drug effect was determined by immunohistochemistry. We have identified a benzodiazepine derivative, KRM-II-08, as a new potent inhibitor in several α 5-GABAA receptor expressing tumor models. This is the first instance of in vivo testing of several benzodiazepine derivatives and standard chemotherapeutic drugs within the same tumor. Obtaining high-throughput drug efficacy data within a native tumor microenvironment as detailed herein, prior to pharmacological optimization for bioavailability or safety and without systemic exposure or toxicity, may allow for rapid prioritization of drug candidates for further pharmacological optimization.

46

POSTER 034

Pharmacokinetic Studies of Drug Candidates for Neurological Disorders and Asthma Based on GABAA Receptor Subtype Selective Ligands

Revathi Kodali,a Nicolas M. Zahn,a Gloria S. Forkuo,a Rashid Roni,a Margaret L. Guthrie,a

Rajwana Jahan,a Guanguan Li,a Charles W. Emala,b James M. Cook,a Douglas Stafford,a and Leggy A. Arnolda

Department of Chemistry & Biochemistry and Milwaukee Institute for Drug Discovery,

University of Wisconsin-Milwaukee bDepartment of Anesthesiology, College of Physicians and Surgeons of Columbia University

Development of pre-clinical experimental models to understand the in vivo performance of a drug is important in the field of drug discovery. GABA-ergic drugs are historically used for the treatment of neurological disorders such as neuropathic pain, schizophrenia, and anxiety but recently have shown potential to treat asthma. In the present study, an in vivo pharmacokinetic study was designed to evaluate the drug absorption, distribution, metabolism, and excretion of GABAA receptor subtype selective ligands using rodent models. An LC-MS/MS method was developed to quantify the amount of drug distributed in different tissues over a period. Herein, we will report the development, analysis, and standardization of a mice pharmacokinetic study using the Shimadzu LCMS-8040 triple quadrupole instrument.

47

POSTER 035

Identifying of the Molecular Target for the Potent Antimicrobial Agent TI-I-100

to Treat Drug Resistance Bacteria

V. V. N. Phani Babu Tiruveedhula,a Revathi Kodali,a Lanlan Han,a Sonia L. Bardy,b Shama P.

Mirza,a Nicholas R. Silvaggi,a Douglas Stafford,a Leggy A. Arnold,a and James M. Cooka

aDepartment of Chemistry & Biochemistry and Milwaukee Institute for Drug Discovery,

University of Wisconsin-Milwaukee bDepartment of Biological Sciences, University of Wisconsin-Milwaukee

The alarming increase in bacterial resistance over the last decade, coupled with the lack of new classes of antibiotics to treat resistant infections, is a growing healthcare threat. In continued efforts to develop new potent antimicrobial agents, a novel class of acrylic esters was synthesized. To determine the molecular target for this class of compounds, the novel alkyne TI-I-100 was designed as a substrate for the copper catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC). Gratifyingly, TI-I-100 exhibited promising MIC values of 0.25-4.0 μg/mL against clinically significant antibiotic-resistant strains like MRSA, MDR VISA, and VRE. Consequently, this stimulated the search for the molecular target employing the CuAAC reaction using the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit with fluorescently tagged Alexa Fluor 647 picolyl azide (AF647) within Staphylococcus aureus lysate. This permitted experiments to identify S. aureus proteins that had been covalently modified by the alkyne, TI-I-100 to be seen on SDS-PAGE under fluorescence. Encouraged by the results, AF647 was replaced with biotin azide in order to isolate the targeted proteins using streptavidin beads. Later, the purified protein fractions were subjected to peptide mass fingerprinting for protein identification and results will be presented.

48

POSTER 036

Novel orally bioavailable GABA A receptor positive allosteric modulator for alleviating multiple asthma symptoms

Gloria S. Forkuo1, Ted Harris William1, Amanda N. Nieman1, Olivia B. Yu, Margaret L. Guthrie1,

Revathi Kodali1, Guanguan Li1, Rajwana Jahan1, Michael R. Stephen1, Charles W. Emala2, James M. Cook1, Douglas Stafford1, and Leggy A Arnold1

1Department of Chemistry and Biochemistry and the Milwaukee Institute for Drug Discovery,

University of Wisconsin-Milwaukee 2Columbia University, College of Physicians and surgeons of Columbia University

Asthma is a chronic inflammatory disease of the airways characterized by airway inflammation, excessive mucous secretion and airway hyper-responsiveness (AHR). γ-Aminobutyric acid (GABA) is an important neurotransmitter, which acts through the subtype A GABA receptor (GABAA receptor) to induce inhibition in the brain. Studies have shown the expression of GABAA receptor subunits in three main cell types known to modulate the pathogenesis of the asthma phenotype namely; inflammatory cells, airway epithelial cells and airway smooth muscles. GABAA receptor activation in inflammatory cells and smooth muscle cells reduce inflammation and relaxes the airway smooth muscles respectively whereas its activation in the epithelial cells increase mucous production. Using novel orally bioavailable GABAA receptor agonists, we investigated their effect on the asthma phenotype in a murine asthma model.

49

POSTER 037

Streptomyces wadayamensis MppP: A Novel PLP-Dependent L-Arginine Hydroxylase in L-Enduracididine Biosynthesis

Lanlan Han and Nicholas Silvaggi

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

The nonproteinogenic amino acid L-enduracididine (L-End) is a critical component of the mannopeptimycins, a family of cyclic glycopeptide antibiotics with potent activities against drug-resistant pathogens like MRSA. Creating derivatives of mannopeptimycin and other L-End-containing natural products is hampered by the limited availability of L-End. We are investigating the L-End biosynthetic pathway in order to develop an efficient enzymatic or chemo-enzymatic route to produce this unusual amino acid. Recently, we found that MppP is a PLP-dependent L-Arg hydroxylase in L-End biosynthesis in Streptomyces wadayamensis. It reacts with L-Arg and dioxygen to yield two products, 2-ketoarginine and 4-hydroxy-2-ketoarginine in a ratio of ~1.7:1. Surprisingly, 1 equivalent of H2O2 is produced for each equivalent of O2 consumption. In addition, the ratio of O2 consumption to L-Arg consumption was ~1.4:1, suggesting that one product requires 2 equivalent of O2, and the other just needs 1 equivalent. We have determined the structures of MppP in four states: the internal aldimine, the external aldimine with the substrate L-Arg, the product complex with 4-hydroxy-2-ketoarginine, and the product complex with 2-ketoarginine. According to the structures, the N terminal helix is disordered in internal aldimine state and covered the active site when the substrate bound. So the mutants E15A/Q and N terminal truncated mutant as well as five point mutants in the active site were made to explore the functional roles of specific residues. NMR and MS results demonstrated that most mutants only catalyze the formation of 2-ketoarginine except E15Q and F191Y. The ratio of the two products of E15Q and F191Y variants were ~1:2.4 and ~1.6:1, respectively. The structures of several mutants E15Q, H29A, H29S, F115Y, and D218S were also obtained. There is no PLP in the active site of D218S mutant. There is no dramatic conformation change for other mutants. The steady state results showed that the catalytic efficiency of those mutants with L-Arg in anaerobic half reaction was similar except D218S and the N terminal truncated mutant. The D218S and the N terminal truncated mutants exhibited a ~20 fold lower catalytic efficiency.

50

POSTER 038

An Immunotoxicity Safety Study of GL-II-93

Alec Huber, Nicolas M. Zahn, Guanguan Li, Md S.R. Roni, and Leggy A. Arnold

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee Asthma is among the most common of health ailments, but established modes of treatment leave much to be desired, and invite novel pharmaceutical approaches. Evidence indicates the feasibility of the use of subunitselective GABAA receptor (GABAAR) agonists as a means of manipulating certain non-neuronal targets, such as airway smooth muscle (ASM) cells. Such compounds have been found to induce relaxation of ASM cells, leading to alleviation of airway constriction. In this study currently in progress, the immunotoxic properties of the subunitselective imidazobenzodiazepine GL-II-93 are evaluated by multiple measures in order to determine whether chronic administration of the compound is conducive to undesired pharmacological effects. The results obtained thus far are discussed.

51

POSTER 039

Synthesis of Benzofuran ring-containing compound BRL-37959 and its analogs

Shamsul Arefin Ahmed, Damon Hinz, and M. Mahmun Hossain

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Synthesis of BRL-37959 has previously been reported. As a NSAID, the compound was tested for, and found that it had, very low gastric irritancy. We have developed a short and more cost-effective procedure for the synthesis of BRL-37959. The old method was tedious and had very low yields (≤ 5%). Our developed method is simple and uses inexpensive starting material, as well as giving high overall yields (up to 89%). Moreover, in molecular modeling (docking) studies it has been found that the compound selectively binds with COX-2 enzymes. Hopefully, the drug will act as a selective COX-2 inhibitor.

52

POSTER 040

Quantification of Calcitroic Acid using Liquid Chromatography Tandem Mass Spectrometry

Elliot Di Milo, Margaret Guthrie, Revathi Kodali, Olivia Yu, and Leggy A. Arnold

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

The physiological role of vitamin D can be better hypothesized with a deeper understanding of the metabolic contribution of calcitroic acid (CTA), one of its biochemical metabolic products. Vitamin D is formed in vivo through a pathway starting with 7-dehydrocholesterol, which is converted into 1,25(OH)2D3. Some vitamin D is metabolized to 24,25(OH)2D3 before forming calcitroic acid. This study is intended to establish the concentration of CTA in mouse blood samples as well as its stability through microsomal stability assays. A method was developed for the quantification of CTA and a series of other bile acid derivatives were explored as internal standards through high pressure liquid chromatography tandem mass spectrometry (HPLC–MS/MS) in multiple reaction monitoring (MRM) mode. Calibration curves of CTA were created and the limit of detection was determined to be 37.3 ng/mL. A microsomal stability assay will be run with both mouse and human models to further understand CTA’s metabolic life span. Blood, and homogenized mouse liver samples will be analyzed in respect their concentrations of CTA.

53

POSTER 041

Novel Strategy for The Treatment of Asthma by Targeting the α4 Subunit of GABAA Receptors in Airway Smooth Muscle

Rajwana Jahan and James M. Cook

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Asthma is a major health concern and millions of individuals are affected. Current asthma treatments, both chronic maintenance and acute rescue therapy, use β2-agonists and corticosteroids, both of which suffer from inadequate efficacy and safety issues. As a result, there is an unmet demand for more effective and safer treatments for asthma. Our previous studies demonstrated that airway smooth muscle (ASM) cells express GABAA receptors (GABAARs) of the α4 and α5 subunits and agonists of GABAAR can relax ASM acutely. Targeting the limited and overlapping α subunits with subtype selective GABAAR agonists would cause both ASM relaxation and suppression of inflammation without having any off-target CNS activity. Bz/GABAAergic agents have been proven to be safe and have a long clinical safety record. As a result targeting Bz/GABAAR in the lung would be a novel and effective strategy in asthma management. Novel GABAAR positive allosteric modulators designed for α4/α6 subunit selectivity were synthesized using iterative computational analyses. Two compounds from our library, namely CMD-45 and XHe-III-74 have shown α4 subtype selectivity and serve as leads for designing novel drugs for treating asthma. Recent results in this area will be presented.

54

POSTER 042

Curriculum Transformation and Student Engagement in General Chemistry 103 and 104

Liana Lamont1, Lindy Stoll1, Clark Landis1,2, and Ned Sibert1,2

1Department of Chemistry, University of Wisconsin-Madison

2REACH Co-Leads (Chemistry Initiative) Our poster describes a major transformation of the 103/104 General Chemistry curriculum occurring on the Madison campus since 2015. This transformation is part of a broad campus educational innovation initiative called REACH (REdesign for ACtive Learning in High Enrollment Courses). The goal of REACH is to transform high-enrollment, lecture-based courses into more active and inclusive learning environments. The initiative will inspire greater student responsibility for learning, increase student inquiry and engagement with the subject matter, and improve student learning. The REACH Initiative in Chemistry, Physics, and Math started a campus-wide effort that now includes eleven courses. Chemistry 103 is in its third semester as a fully transformed course, and Chemistry 104 is in its first semester as a fully transformed course. Course redesign is iterative, and we continue to improve both courses. The Chemistry curriculum transformation has occurred both within and outside of the classroom. Our poster describes the newly designed courses as well as the challenges faced in the creation of this course sequence.

55

POSTER 043

Rethinking science teacher preparation in face of changing challenges – The Milwaukee Master Teacher Partnership (MMTP)

Anja Blecking

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

K-12 science education has undergone significant changes over the last decade, along with any educational shift, teacher expectations have changed as well. The adaptation of the Next Generation Science Standards in one of the largest public urban school districts in the Midwest, the Milwaukee Public Schools, requires teachers to rethink their practices to effectively implement the three dimensions, Scientific and Engineering Practices, Crosscutting Concepts, and Disciplinary Core Ideas into their science classrooms. As one of the certifying institutions in the area, UW-Milwaukee strives to effectively prepare future science teachers for these challenges and also work with in-service teachers to effectively transition to the new standards. This presentation describes the current 5-year collaboration between the Milwaukee Public Schools, UWM College of Letters and Science, and the UWM School of Education working with MPS mathematics and science teachers.

56

POSTER 044

Qualitative Analysis of the Impact of Classroom Salon on the Exam Item Performance of Introductory Chemistry Students

Bridgette Walters and Anja Blecking

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Classroom Salon (CLS) is a social online learning platform that has been developed by educators at Carnegie Mellon University. The platform allows students to interactively comment and discuss ideas, texts, videos, news articles and other reading material. Classroom Salon has been implemented into introductory chemistry classes at UW-Milwaukee in 2012 as delivery method for content reading assignments prior to covering the content in class. Students comment and ask questions about the text which then allows instructors to directly incorporate these comments into instruction. As part of the assigned reading, students were also asked to answer questions that were embedded in the text and which were meant to promote critical thinking skills. Quantitative analysis has shown that students who utilize CLS perform on average higher in exams than students who enrolled in a different lecture section of the course and were assigned the same reading on paper. As an extension of this research, this project describes a more detailed analysis of specific final exam items that correlate directly with each of the ten content areas of the reading assignments. Items were rated according their complexity by expert raters, compared to the questions in the reading assignments, and then student performance on these items was compared. The analysis is meant to reveal strength and shortcomings of this methodology and efficacy of the reading assignments as well as suggest possible changes to improve student course performance in the future.

57

POSTER 045

Solid state analysis of dihedral angles in (halobiphenyl) chromium tricarbonyl complexes

Curtis J. Czerwinski*, Ilia A. Guzei†, Lara C. Spencer†, Sondra C. Buechel*, and Leah B. Kaufmann*

*Department of Chemistry, University of Wisconsin-La Crosse †Department of Chemistry, University of Wisconsin-Madison

A series of halogen-substituted (biphenyl)chromium(0)tricarbonyl complexes have been characterized by single-crystal X-ray diffraction. Syntheses of these compounds involved heating (2-X-biphenyl) or (4-X-biphenyl) (X = F, Cl, Br) with Cr(CO)6. These conditions led to formation of (biphenyl)chromium(0)tricarbonyl complexes where in all cases the non-halogenated ring became ligated to the metal center. A series of solid state structures of (2-X-biphenyl)Cr(CO)3 (X = H, F, Cl, Br) shows a notable trend in increasing arene-arene dihedral angles that may be rationalized through a combination of halogen size and carbon-halogen bond length. Special emphasis was placed on fluorinated derivatives, where two (fluorobiphenyl)-chromium(0)tricarbonyl complexes, as well as their respective free ligands, were characterized by single-crystal X-ray diffraction. In the solid state [(η6-C6H5)(2-F-C6H4)]Cr(CO)3 exhibits a 55.77(4)° dihedral angle, and [(η6-C6H5)(4-F-C6H4)]Cr(CO)3 exhibits a 52.4(5)° dihedral angle. While the angle for the ortho-fluoro complex is not unexpected, the angle for the para-fluoro complex is surprisingly large, especially since the solid state structures of the free ligands (2-F-biphenyl) and (4-F-biphenyl) exhibit arene–arene dihedral angles of 54.83(7)° and 0.71(8)°, respectively. Structural features of these complexes are compared and contrasted to those of DFT-optimized geometries of ten [(η6-(C6H5)(4-F-C6H4)]chromium(0) tricarbonyl model complexes. Packing diagrams for the solid-state structures reveal the presence of intermolecular C-H…F non-classical hydrogen bonding, which may be the basis for the large dihedral angles that are observed in the organometallic complexes.

58

POSTER 046

Real-Time Monitoring of Heavy Metal Ions in Wastewater

Tyler Fenske, Sarah A. Oehm, Trevor Hagemann, Paul Henning, Joe Labeots, Alan W.

Schwabacher, and Peter Geissinger

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee Quantitation of heavy metal ions in wastewater by atomic absorption spectroscopy has drawbacks. Due to the high cost of the instrumentation, off-site analysis causes lag time between sampling and results. The development of a method for the on-site and real-time quantitation of multiple heavy metal in wastewater is highly desirable for industrial facilities because it can allow for a more precise treatment of the wastewater produced. Herein we describe the development of hydrogel polymer sensors that are capable of binding to metal ions from solution, resulting in a change of UV/Vis absorbance profiles. These sensors are robust, reversible, and provide information on the solution composition in real-time. Although each sensor exhibits only a semi-specific response for each metal ion, a collection of these different sensors can be used as a multiplex sensor array that can produce specific metal responses and is capable of elucidating the composition of an aqueous solution containing a variety of metal ions.

59

POSTER 047

Substituent Effects on Photo-induced Cross-Link in DNA Formation by Naphthalene Precursors via a Carbocation

Zechao Lin, Qi Zhang and Xiaohua Peng

Department of Chemistry & Biochemistry, University of Wisconsin-Milwaukee

Photochemical DNA-cross-linking agents have found wide biological applications. Very recently, several bifunctional aryl boronates have been reported to induce DNA interstrand cross-link (ICL) via carbocations upon photoirradiation. However, it is mainly limited to bifunctional aryl boronates compounds. In addition, these bifunctional naphthalene boronates showed very low DNA cross-linking efficiency. Herein, we synthesized a series of bifunctional naphthalene compounds with various aromatic substituents and leaving groups in order to study the effect of structure variation on the DNA cross-linking efficiency and the reaction mechanism. Although compounds bearing different aromatic substituents and/or different leaving groups showed a different reactivity toward DNA, all compounds can be activated by 350 nm irradiation to induce DNA ICL formation via carbocations. The alkylation sites were dG, dA, and dC, which were determined by studying the heat stability of the ICL products as well as by isolating and characterizing the adducts formed between the naphthalene cations and four natural nucleotides.

60

POSTER 048

CHOPS/PGSEC: Chemistry Opportunities/Partners for Graduate School Experiences

Desiree Bates, Arrietta Clauss, Michael Schwartz, and Rob McClain

Department of Chemistry, University of Wisconsin-Madison

The Department of Chemistry at the University of Wisconsin-Madison has two recruiting programs targeting talented students from underrepresented groups, who might not have considered graduate school in chemistry as a career option. For these programs, CHOPS and PGSEC, we invite about 20 students to our campus for an all-expense paid weekend, where we highlight life as a Ph.D student in chemistry. During the weekend, these students meet current graduate students, have one-on one meetings with selected faculty, and attend presentations about chemistry research, careers options for Ph.D chemists, and applying to graduate school. We would like to use this poster session to share our experiences from these programs with faculty at the University of Wisconsin campuses. We hope these faculty will help our recruiting efforts, especially with students from within the state of Wisconsin.

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POSTER 049

Lab Demonstration of the Kinetics for the Hydrogenation of 1-Octene

Dana C Haagenson

University of Wisconsin-Marshfield/Wood County

A series of 1-octene hydrogenations catalyzed by Pd/C are performed by the instructor. The results are presented to the class and discussed. The experiments and discussion will be presented.

62

POSTER 050

Annual Wisconsin Crystal Growing Contest

Ilia A. Guzei

Department of Chemistry, University of Wisconsin–Madison The state-wide Wisconsin Crystal Growing Contest is open to middle and high school students ages 11-18 as well as home-schooled youths and science teachers. The objective of the Contest is to introduce the students to the scientific method, chemistry, crystallography, and optical microscopy. The students compete for prizes by growing large, high quality crystals of safe chemical compounds that are evaluated and ranked by a panel of professional chemists. This engaging cross-curriculum activity allows students to learn an advanced vocabulary and essential laboratory skills such as working safely, creatively and collaboratively, while keeping lab records of their experiments. The contestants are invited to visit the University of Wisconsin-Madison Chemistry department to attend the award ceremony, listen to lectures, observe and participate in hands-on chemical demonstrations, and appreciate how scientific research is conducted at a flagship university. The participants visit the teaching and research laboratories, X-ray crystallography laboratory and glass-blowing shop.

63

POSTER 051

Corral Trapping of Nanoparticles in Solution

Xavier S. Udad, Christine A. Carlson, and Jörg C. Woehl

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee The controlled manipulation of nanoscale objects (and particularly of single molecules) is one of the great outstanding challenges for the fabrication of nanosensors and nanodevices that will drive future technologies. Any such interaction requires an efficient and reliable method for confining these objects on demand and in a non-invasive way, which is a formidable task in the case of single molecules. Our lab has developed a trapping technique based on the creation of an electric potential well in solution placed above a circular, non-conductive area in a thin metal layer (corral trap), and successfully trapped single molecular ions (DNA fragments) and other charged micro- and nanoparticles in dilute, aqueous electrolyte solutions. We will outline trap fabrications methods, present some recent experimental results using both ADC and AC fields, and discuss mechanisms for corral trapping (dielectrophoretic vs. electrostatic).

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POSTER 052

Fostering Undergraduate Research Skills

Cheri A. Barta

Department of Chemistry, University of Wisconsin-Madison

Undergraduate research has been shown to be instrumental in the retention and persistence of students in the STEM discipline. Teaching undergraduate students how to become effective researchers, however, can be challenging. At UW-Madison, a year-long course has been designed to help guide beginning undergraduate research students. The first semester of the course gives students insights into how to find research on campus and provides the support needed for students to be successful in their pursuits. Basic research skills are also discussed such as how to read scientific literature and how to document their work. The second semester of the course focuses on communication skills to a variety of audiences. Concurrently, mentors also have the opportunity to engage in a workshop series aimed at introducing evidence-based mentoring best-practices to help foster positive research experiences for both the undergraduate mentee and for the research mentor. Topics for the two courses and for the mentoring workshop will be shared.

65

POSTER 053

Towards a super-resolution map of the electric double layer

Quintus Owen and Jörg C. Woehl

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee The formation of an electric double layer (EDL) at the interface between charged surfaces and an electrolyte solutions is crucial for understanding the properties of colloidal systems and biomolecules in solution. It is also of great interest when electrostatic forces are employed to manipulate charged particles in dilute electrolyte solutions, such as in the case of the (DC) corral trap, a trapping technique developed in our group that relies on electrostatic interactions between a charged surface and similarly charged nanoparticles or ions in solution. The formation of an EDL effectively shields the charges brought to the surface, and as a result diminishes the strength of the electrostatic potential well in solution. To analyze the formation and concentration profile of the EDL, we will employ super-resolution microscopy to image the locations of charged, fluorescent particles in solution between two electrodes patterned onto a glass coverslip before, during, and after the application of an electric voltage. The height of the electrode walls must be smaller than the axial extension of the point spread function of the optical imaging system to ensure that all particles remain in focus during the experiments. We will present fabrication methods and first experimental results, discuss some classical EDL theories and their shortcomings, and offer a more adequate theoretical description of the EDL in finite systems.

66

POSTER 054

A Simple and Short Laboratory Practice on Chemical Equilibria and Kinetics: The Tetramethyl Rosamine/Sodium Hydroxide Reaction

Yiming Yang and Guilherme L. Indig

Department of Chemistry and Biochemistry, University of Wisconsin-Milwaukee.

(Undergraduate Research – Spring 2017) The reaction of the fluorescent dye tetramethyl rosamine (TMR+) with sodium hydroxide in aqueous media leads to the formation of the respective carbinol base (TMR-OH). Because addition of OH- breaks the conjugation in the parent dye cation, the reaction product does not absorb light (and does not fluoresce) in the visible region of the spectrum. In this study we have used visible spectroscopy to track the progress of this reaction at room temperature. Here the

use of the initial rates method, applied under conditions of pseudo-first order ([OH-] >> [TMR])

has provided for the characterization (in just about one hour of laboratory work) of the reaction order with respect to each reactant (and therefore the overall reaction order) and the characterization of the rate constant associated with the forward reaction. Besides, because under selected experimental conditions (e.g. [OH-] = 0.5 M; [TMR] = 10-5 M) reaction equilibria is reached in just about 15 minutes of reaction time, the equilibrium constant and rate constant associated with the reverse reaction were also easily and conveniently characterized within the time frame available to most undergraduate laboratory practices. Therefore, this reaction may find good use as a novel undergraduate physical chemistry laboratory practice aimed at the simple and fast characterization of reaction orders, rate constants, and equilibrium constant. Additionally, because TMR+ is a highly fluorescent dye, this practice can also be used to introduce fluorescence spectroscopy to undergraduate students.

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The 44th Annual UW System Chemistry Faculties Meeting

UW Chemistry Faculty Directory

Amy Kabrhel, UW-Manitowoc, Associate Professor (Chemistry Dept. Chair for UW Colleges), amy.kabrhel@uwc.edu , 920-683-2746

705 Viebahn St., Manitowoc, WI 54220 www.uwc.edu/depts/chemistry

Baraboo/Sauk County Stephen Swallen Asst. Lecturer stephen.swallen@uwc.edu 608-355-5226

Barron County Abbey Fischer Asst. Professor abbey.fischer@uwc.edu 715-234-8176

Fond du Lac Roger Traxinger Senior Lecturer roger.traxinger@uwc.edu 920-929-1160

Ronald Theys Assoc. Professor ronald.theys@uwc.edu 920-929-1159

Fox Valley Caroline Geary Professor caroline.geary@uwc.edu 920-832-2851

Peter Gibson Senior Lecturer peter.gibson@uwc.edu 920-832-2654

Rose Marie Lewis Laboratory Technician rosemarie.lewis@uwc.edu 920-832-2654

Martin Rudd Professor and Regional CEO/Dean

martin.rudd@uwc.edu 920-832-2612

Brian Rukamp Senior Lecturer brian.rukamp@uwc.edu 920-832-2654

Kimberly Schatz Professor kimberly.schatz@uwc.edu 920-832-2855

Manitowoc Amy Kabrhel Assoc. Professor, Chair amy.kabrhel@uwc.edu 920-683-2746

Tracy Smith Leiker Senior Lecturer tracy.smithleiker@uwc.edu 920-683-4736

Breeyawn Lybbert Asst. Professor breeyawn.lybbert@uwc.edu 920-683-2746

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Marathon County Kaitlyn Mahoney Asst. Professor kaitlyn.mahoney@uwc.edu 715-261-6319

Scott Sargent Instructional Specialist/ Associate Lecturer

scott.sargent@uwc.edu 715-261-6315

Penny Workman Assoc. Professor penny.workman@uwc.edu 715-261-6316

Marinette Mark Klemp Assoc. Professor mark.klemp@uwc.edu 715-735-4329

Marshfield/Wood County Dana Haagenson Assoc. Professor dana.haagenson@uwc.edu 715-389-6526

Online Thomas Franz Assoc. Lecturer thomas.franz@uwc.edu 715-234-8176

Anthony Millevolte Professor anthony.millevolte@uwc.edu 715-234-8176

Gail Vojta Senior Lecturer gail.vojta@uwc.edu

Richland Brandon Fetterly Assoc. Professor Brandon.fetterly@uwc.edu 608-647-6186 x103

Rock County Michael Gorman Lecturer michael.gorman@uwc.edu 608-758-6531

Kim Kostka Professor kim.kostka@uwc.edu 608-758-6532

Kristin Plessel Assoc. Professor kristin.plessel@uwc.edu 608-758-6569

Eric Stoner Lecturer eric.stoner@uwc.edu 608-758-6565

Sheboygan James Kabrhel Assoc. Professor james.kabrhel@uwc.edu 920-459-4427

Karrie Rukamp Senior Lecturer karrie.rukamp@uwc.edu 920-459-6626

Washington County Mohamed Ayoub Professor mohamed.ayoub@uwc.edu 262-335-5250

Erin O’Connell Assoc. Lecturer erin.oconnell@uwc.edu 262-335-5262

Talitha Selby Assoc. Professor talitha.selby@uwc.edu 262-335-5250

Waukesha Jeremiah Erickson Lecturer jeremiah.d.erickson@uwc.edu 262-521-5526

Asif Habib Professor asif.habib@uwc.edu 262-521-5526

David Olszewski Lecturer david.olszewski@uwc.edu 262-521-5216

Phillip Owens Asst. Professor phillip.owens@uwc.edu 262-521-5524

69

UW-Eau Claire Department of Chemistry

Patricia Benson, Academic Department Associate, jennemp@uwec.edu, 715-836-3417 430 Phillips Science Hall, 105 Garfield Ave., Eau Claire, WI 54701

www.uwec.edu/chemistry

Scott Bailey-Hartsel Professor, Biochemistry hartsesc@uwec.edu 715-836-4746

Sudeep Bhattacharyay Assoc. Professor, Physical/Computational bhattas@uwec.edu 715-836-2278

James Boulter Assoc. Professor, Analyt./Environ. & Watershed Inst. Dir.

boulteje@uwec.edu 715-836-4175

Michael Carney Professor, Inorganic & Assoc. V. Chancellor carneymj@uwec.edu 715-836-4353

Patricia Cleary Asst. Professor, Analytical/Environmental clearypa@uwec.edu 715-836-5518

Bart Dahl Assoc. Professor, Organic dahlbj@uwec.edu 715-836-4179

Jennifer Dahl Assoc. Professor, Inorganic/Nanotechnology dahljenn@uwec.edu 715-836-5788

Stephen Drucker Professor, Physical druckers@uwec.edu 715-836-5390

Warren Gallagher Professor, Biochemistry/Biophysical (Chair) wgallagh@uwec.edu 715-836-5388

*Elizabeth Glogowski Asst. Professor, Organic/Polymer glogowem@uwec.edu 715-836-3445

Jason Halfen Professor, Inorganic halfenja@uwec.edu 715-836-4360

Sanchita Hati Professor, Biochemistry hatis@uwec.edu 715-836-3850

Fred King Professor, Physical fking@uwec.edu 715-836-3744

David Lewis Professor, Organic lewisd@uwec.edu 715-836-4744

*Laurel McEllistrem Analytical Scientist mcellilj@uwec.edu 715-836-2723

*Marc McEllistrem Professor/Director, Material Science & Engineering

mcellimt@uwec.edu 715-836-4081

Cheryl Muller Assoc. Professor, Organic mullercl@uwec.edu 715-836-4019

James Phillips Professor, Physical/Environmental phillija@uwec.edu 715-836-5399

Chris Rhode Senior Lecturer, General rohderc@uwec.edu 715-836-5736

Denis Setwyn Chemical Stockroom Manager setwyndj@uwec.edu 715-836-5369

Roslyn Theisen Asst. Professor, Inorganic/Bioinorganic theiserm@uwec.edu 715-836-4334

Kurt Wiegel Professor, Organic/Polymer wiegelkn@uwec.edu 715-836-4815

Thao Yang Professor, Biochemistry yangt@uwec.edu 715-836-4190

*Materials Science Faculty with Chemistry Emphasis

70

UW-Green Bay Department of Chemistry

Janet Ludke, Academic Department Associate, ludkej@uwgb.edu, 920-465-2371 2420 Nicolet Drive, ES 317, Green Bay, WI 54311

www.uwgb.edu/chemistry

Mandeep Bakshi Assoc. Professor, Chemistry bakshim@uwgb.edu 920-465-5169

Franklin Chen Assoc. Professor, Physical chenf@uwgb.edu 920-465-2834

Georgette Heyrman Assoc. Professor, Biochemistry heyrmang@uwgb.edu 920-465-2275

Jeremy Intemann Assoc. Professor, Organic intemanj@uwgb.edu 920-465-2796

Michael McIntire Assoc. Professor, Physical (Chair) mcintirm@uwgb.edu 920-465-5131

Debra Pearson Assoc. Professor, Nutritional Biochemistry

pearsond@uwgb.edu 920-465-2280

Nydia Villanueva Senior Lecturer villanun@uwgb.edu 920-465-2991

Julie M. Wondergem Assoc. Professor, Organic wondergj@uwgb.edu 920-465-5173

Michael Zorn Professor, Analytical/Environmental zornm@uwgb.edu 920-465-5758

71

UW-La Crosse Department of Chemistry and Biochemistry Lori Hanson, Academic Department Associate, lhanson@uwlax.edu, 608-785-8268

1725 State Street, La Crosse, WI 54601 www.uwlax.edu/Chemistry-and-Biochemistry

Keith Beyer Professor, Physical kbeyer@uwlax.edu 608-785-8292

Basudeb Bhattacharyya Assoc. Lecturer, Biochemistry bbhattacharyya@uwlax.edu 608-785-5275

Jeff Bryan Professor, Nuclear/ Radiochemistry/Inorganic

jbryan@uwlax.edu 608-785-8271

Nadia Carmosini Asst. Professor, Environmental/Analytical

ncarmosini@uwlax.edu 608-785-5276

Tanya Cordes Lecturer, Organic tcordes@uwlax.edu 608-785-8279

Curtis Czerwinski Professor, Organic cczerwinski@uwlax.edu 608-785-8701

Ressano DeSouza-Machado

Senior Lecturer, Analytical/Instrumental

rdesouza-machado@uwlax.edu

608-785-8273

Kate Friesen Lecturer, Analytical kfriesen@uwlax.edu 608-785-5277

Roghaieh (Pari) Ghodsian

Senior Lecturer, General/Analytical rghodsian@uwlax.edu 608-785-8280

Kelly Gorres Asst. Professor, Biochemistry kgorres@uwlax.edu 608-785-5284

Daniel Grilley Asst. Professor, Biochemistry dgrilley@uwlax.edu 608-785-5278

Sandra Grunwald Professor, Biochemistry sgrunwald@uwlax.edu 608-785-8265

Benjamin Haenni Assoc. Lecturer, Organic bhaenni@uwlax.edu 608-785-5285

Justin Jens Lab Manager I jjens@uwlax.edu 608-785-8277

Janet Kirsch Assoc. Professor, Physical jkirsch@uwlax.edu 608-785-8655

Sandra Koster Senior Lecturer, Organic skoster@uwlax.edu 608-785-8282

Daniel Little Assoc. Lecturer, Inorganic dlittle@uwlax.edu 608-785-5283

Adrienne Loh Professor, Physical/Biophysical/NMR

aloh@uwlax.edu 608-785-8275

John May Asst. Professor, Biochemistry jmay@uwlax.edu 608-785-5281

Robert McGaff Professor, Inorganic rmcgaff@uwlax.edu 608-785-8656

Nicholas McGrath Asst. Professor, Organic nmcgrath@uwlax.edu 608-785-8287

Aaron Monte Professor, Organic/Medicinal (Chair)

amonte@uwlax.edu 608-785-8260

Kendric Nelson Asst. Professor, Analytical/Inorganic knelson@uwlax.edu 608-785-8263

Joshua Neukom Assoc. Lecturer, Organic jneukom@uwlax.edu 608-785-8278

Aric Opdahl Professor, Surface/Analytical/ Instrumentation

aopdahl@uwlax.edu 608-785-8274

Kris Rolfhus Professor, Analytical/Environmental krolfhus@uwlax.edu 608-785-8289

Heather Schenck Professor, NMR Spectroscopy/Organic

hschenck@uwlax.edu 608-785-8288

Valeria Stepanova Asst. Professor, General vstepanova@uwlax.edu 608-785-8267

Yevgeniya Turov Assoc. Lecturer, General/Inorganic yturov@uwlax.edu 608-785-5282

Todd Weaver Professor, Biochemistry tweaver@uwlax.edu 608-785-8269

72

UW-Madison Department of Chemistry

Kayla Driscoll, Assistant to the Chair, kayla.driscoll@wisc.edu, 608-262-1480 1101 University Avenue, Madison, WI 53706-1322

www.chem.wisc.edu

John F. Berry Assoc. Professor, Inorganic/Organic berry@chem.wisc.edu 608-262-7534

Tim Bertram Assoc. Professor, Analytical/Physical tbertram@chem.wisc.edu 608-890-3422

Helen Blackwell Professor, Chemical Biology/Organic blackwel@chem.wisc.edu 608-262-1503

Stephen Block General Chemistry Lab Director sblock@chem.wisc.edu 608-262-1511

Thomas Brunold Professor, Chemical Biology/ Inorganic

brunold@chem.wisc.edu 608-265-9056

Andrew Buller Asst. Professor, Chemical Biology/ Organic

arbuller@wisc.edu 608-265-8431

Steve Burke Professor, Organic burke@chem.wisc.edu 608-262-4941

Judith N. Burstyn Professor, Inorganic (Chair) burstyn@chem.wisc.edu 608-262-0328

Silvia Cavagnero Professor, Analytical/Chemical Biology/Organic/Physical

cavagner@chem.wisc.edu 608-262-5430

Kyoung-Shin Choi Professor, Analytical/Inorganic/Materials

kschoi@chem.wisc.edu 608-262-5859

Joshua J. Coon Professor, Analytical/Chemical Biology/Biochemistry

jcoon@chem.wisc.edu 608-263-1718

Qiang Cui Professor, Physical/Chemical Biology cui@chem.wisc.edu 608-262-9801

Pamela Doolittle Analytical Chemistry Lab Director pam@chem.wisc.edu 608-262-9679

Mark D. Ediger Professor, Materials/Physical ediger@chem.wisc.edu 608-262-7273

Brian Esselman Organic Lab Director besselman@chem.wisc.edu 608-262-1479

Katrina Forest Professor, Chemical Biology forest@bact.wisc.edu 608-265-3566

Daniel C. Fredrickson Assoc. Professor, Inorganic/Materials

danny@chem.wisc.edu 608-890-1567

Charles G. Fry Senior Scientist, NMR Director fry@chem.wisc.edu 608-262-3182

Etienne Garand Asst. Professor, Physical egarand@chem.wisc.edu 608-890-4905

Ying Ge Assoc. Professor, Cell & Regenerative Bio & Chem

ge2@wisc.edu 608-263-9212

Samuel H. Gellman Professor, Organic gellman@chem.wisc.edu 608-262-3303

Pupa Gilbert Professor, Physics/Chemistry pupa@physics.wisc.edu 608-262-5829

Jennifer Golden Asst. Professor, Pharmacy Jennifer.golden@wisc.edu 608-263-7287

Randall Goldsmith Asst. Professor, Materials/Physical rhg@chem.wisc.edu 608-263-8315

Padma Gopalan Professor, Materials/Organic pgopalan@wisc.edu 608-265-4258

Ilia A. Guzei Senior Scientist, Crystallography iguzei@chem.wisc.edu 608-263-4694

Jeanne Hamers Undergraduate Chemistry Director jhamers@chem.wisc.edu 608-263-4852

Robert J. Hamers Professor, Analytical/Materials/Physical

hamers@chem.wisc.edu 608-262-6371

73

Ive Hermans Assoc. Professor hermans@chem.wisc.edu 608-262-4966

Nicholas Hill Organic Lab Director Hill@chem.wisc.edu 608-262-2306

Heike Hofstetter Asst. NMR Director hofstetter@chem.wisc.edu 608-262-7536

Richard Hsung Professor, Organic rphsung@pharmacy.wisc.edu 608-890-1063

Catherine Jackson Asst. Professor, History of Science Cjackson8@wisc.edu NA

Song Jin Professor, Analytical/Inorganic/ Materials/Physical

jin@chem.wisc.edu 608-262-1562

Thomas Kuech Professor, Chem & Biological Engr kuech@engr.wisc.edu 608-263-2922

Clark Landis Professor, Inorganic/Organic landis@chem.wisc.edu 608-262-0362

Lingjun Li Professor, Analytical lli@pharmacy.wisc.edu 608-265-8491

David Lynn Professor, Organic dlynn@engr.wisc.edu 608-262-1086

Rob McClain Analytical Instruments Lab Director mcclain@chem.wisc.edu 608-262-5615

Robert J. McMahon Professor, Materials/Organic mcmahon@chem.wisc.edu 608-262-0660

Sandro Mecozzi Assoc. Professor, Organic mecozzi@chem.wisc.edu 608-262-7810

Cathy Middlecamp Professor, Gaylord Nelson Inst. Environ. Study

cathy@chem.wisc.edu 608-263-5647

John W. Moore Professor, Inorganic jwmoore@chem.wisc.edu 608-262-5154

Gilbert Nathanson Professor, Physical nathanso@chem.wisc.edu 608-262-8098

Joel A. Pedersen Professor, Soil Science joelpedersen@wisc.edu 608-263-4971

Thomas Record Professor, Organic/Physical/Chemical Biology

record@chem.wisc.edu 608-262-5332

Jordan J. (JR) Schmidt Assoc. Professor, Physical schmidt@chem.wisc.edu 608-262-2996

Jennifer Schomaker Assoc. Professor, Organic schomakerj@chem.wisc.edu 608-265-2261

David Schwartz Professor, Analytical schwartz@chem.wisc.edu 608-265-0546

Bassam Z. Shakhashiri Professor, Inorganic bassam@chem.wisc.edu 608-262-0538

Ned Sibert Professor, Physical sibert@chem.wisc.edu 608-262-0265

Lloyd M. Smith Professor, Analytical/Materials smith@chem.wisc.edu 608-263-2594

Shannon Stahl Professor, Inorganic/Organic stahl@chem.wisc.edu 608-265-6288

Weiping Tang Assoc. Professor, Medicinal Chemistry

wtang@pharmacy.wisc.edu 608-890-1846

Martha M. Vestling Senior Scientist, Mass Spec vestling@chem.wisc.edu 608-262-3449

Douglas B. Weibel Professor weibel@biochem.wisc.edu 608-890-1342

James C. Weisshaar Professor, Analytical/Physical weisshaa@chem.wisc.edu 608-262-0266

Daniel Weix Assoc. Professor Inorganic / Organic dweix@wisc.edu 608-262-0421

Mark Wendt Physical Chemistry Lab Director wendt@chem.wisc.edu 608-262-0806

Chad Wilkinson General and Inorganic Chemistry Lab Director

wilkinson@chem.wisc.edu 608-263-4851

Claude Woods Professor, Physical woods@chem.wisc.edu 608-262-2892

John C. Wright Professor, Analytical/Materials/Physical

wright@chem.wisc.edu 608-262-0351

Arun Yethiraj Professor, Materials/Physical yethiraj@chem.wisc.edu 608-262-0258

Tehshik P. Yoon Professor, Inorganic/Organic tyoon@chem.wisc.edu 608-262-2268

Lian Yu Professor, Physical lyu@pharmacy.wisc.edu 608-263-2263

Martin Zanni Professor, Physical zanni@chem.wisc.edu 608-262-4783

74

UW-Milwaukee Department of Chemistry and Biochemistry

Wendy Grober, Assistant to the Chair, wgrober@uwm.edu , 414-229-4098 3210 N. Cramer Street, Milwaukee, WI 53211-3029

www.uwm.edu/chemistry

Joseph Aldstadt Assoc. Professor, Analytical/Instrumentation

aldstadt@uwm.edu 262-689-2766

Charles Allen Lecturer ceallen@uwm.edu 414-229-4899

Alexander (Leggy) Arnold

Assoc. Professor, Organic/Medicinal/Drug Disc.

arnold2@uwm.edu 414-229-2612

Anja Blecking Asst. Professor, Chemical Education blecking@uwm.edu 414-229-2974

Christine Carlson Senior Lecturer cac4@uwm.edu 414-229-4411

Jian Chen Assoc. Professor, Organic/Medicinal, Materials/Nano

jianchen@uwm.edu 414-229-6464

James M. Cook Dist. Professor, Organic/Medicinal, Drug Disc.

capncook@uwm.edu 414-229-5856

Mark L. Dietz Professor, Analytical/Instrumentation dietzm@uwm.edu 414-229-1748

Holger F. Foersterling Director of the NMR Laboratory holger@uwm.edu 414-229-5896

Gloria Freschl Senior Lecturer freschl@uwm.edu 414-229-3759

David N. Frick Professor, Biological/Structural Bio., Drug Disc.

frickd@uwm.edu 414-229-6670

M. Mahmun Hossain Professor, Organic/Medicinal, Drug Disc. mahmun@uwm.edu 414-229-6698

Matthew Huisman Laboratory Manager mhuisman@uwm.edu 414-229-4383

Guilmerme L. Indig Assoc. Professor, Biological/Structural, Drug Disc.

glindig@uwm.edu 414-229-5034

Neal Korfhage Scientific Glassblower korfhage@uwm.edu 414-229-5224

Leonid Lerner Instr. Innovator, Director of the Research Instrumentation Laboratory

lernerl@uwm.edu 414-229-5032

Vincent Maberry Laboratory Technician vmaberry@uwm.edu 414-229-4460

Shama Mirza Asst. Professor mirza@uwm.edu 414-229-3658

Graham R. Moran Professor, Biological/Structural (Chair) moran@uwm.edu 414-229-5031

Kristen Murphy Assoc. Professor, Chemical Education kmurphy@uwm.edu 414-229-4468

A. Andrew Pacheco Professor, Biological/Structural apacheco@uwm.edu 414-229-4413

Xiaohua Peng Assoc. Professor, Organic/Medicinal, Drug Disc.

pengx@uwm.edu 414-229-5221

David H. Petering Dist. Professor, Biological/Structural, Drug Disc.

petering@uwm.edu 414-229-5853

Alan W. Schwabacher Assoc. Professor, Organic/Medicinal, Drug Disc.

awschwab@uwm.edu 414-229-4410

Nicholas Silvaggi Assoc. Professor, Biological/Structural, Biophys., Drug Disc.

silvaggi@uwm.edu 414-229-2647

Morgan Smith Laboratory Technician smit2774@uwm.edu 414-229-5418

Thomas E. Sorensen Senior Lecturer tes@uwm.edu 414-229-4012

75

Doug Stafford Director of Milwaukee Institute for Drug Disc.

dcstaff@uwm.edu 414-229-5489

Kristene K. Surerus Assoc. Professor, Biological/Structural, Biophys.

surerus@uwm.edu 414-229-2977

Wilfred T. Tysoe Dist. Professor, Materials/Nano wtt@uwm.edu 414-229-5222

Jorg C. Woehl Assoc. Professor, Analy./Instr., Biophys., Materials/Nano

woehl@uwm.edu 414-229-5223

76

UW-Oshkosh Department of Chemistry

Peggy Adamski, Academic Department Associate, adamski@uwosh.edu, 920-424-1400 800 Algoma Blvd., Oshkosh, WI 54901

www.uwosh.edu/chemistry

Christopher Bianchetti Asst. Professor, Biochemistry bianchec@uwosh.edu 920-424-2039

Kevin Crawford Professor, Analytical crawfork@uwosh.edu 920-424-7433

Michael Foley Lecturer, Organic foleym@uwosh.edu 920-424-1314

Jonathan Gutow Professor, Physical gutow@uwosh.edu 920-424-1326

Sharon Hawi Senior Lecturer, Analytical hawi@uwosh.edu 920-424-1029

Brant Kedrowski Professor, Organic kedrowsk@uwosh.edu 920-424-3488

Sheri Lense Asst. Professor, Inorganic lenses@uwosh.edu 920-424-3476

Jennifer Mihalick Professor, Physical (Chair) mihalick@uwosh.edu 920-424-7095

George Vater Olsen Senior Lecturer, Analytical olsengp@uwosh.edu 920-424-2398

James Paulson Professor, Biochemistry paulson@uwosh.edu 920-424-7100

Greg Potratz Stockroom Manager potratzg@uwosh.edu 920-424-1488

Jennifer Schuttlefield Christus Assoc. Professor, Analytical schuttlj@uwosh.edu 920-424-7101

Yijun Tang Assoc. Professor, Analytical tangy@uwosh.edu 920-424-7097

William Wacholtz Professor, Inorganic wacholtz@uwosh.edu 920-424-1482

Lauren Waters Asst. Professor, Biochemistry watersl@uwosh.edu 920-424-7099

Carol Willihnganz Lecturer, Biochemistry willihnc@uwosh.edu 920-424-7093

Linfeng Xie Professor, Organic xie@uwosh.edu 920-424-0436

77

UW-Parkside Department of Chemistry

Nicole Ryan, Academic Department Associate, sturdeva@uwp.edu, 262-595-2316 900 Wood Road, PO Box 2000, Kenosha, WI 53141-2000 www.uwp.edu/learn/departments/chemistry/index.cfm

Lori B. Allen Assoc. Professor (Chair) allen@uwp.edu 262-595-3420

Tsun-Mei (Alice) Chang

Assoc. Professor chang@uwp.edu 262-595-2426

Steve Doelder Assoc. Lecturer doelder@uwp.edu

Jolene Jarrett Chemistry Lab Manager wall@uwp.edu 262-595-2633

Yujuan Liu Lecturer liuy@uwp.edu

Jozef Magonski Senior Lecturer magonski@uwp.edu

Francis Mann Asst. Professor mannf@uwp.edu

Emmanuel Otu Professor (Dean, College of Natural & Health Sci.) otu@uwp.edu 262-595-2977

Daryl Sauer Asst. Professor sauer@uwp.edu 262-595-2430

Gary M. Wood Assoc. Professor woodg@uwp.edu

78

UW-Platteville Department of Chemistry

Kristina Foley, Administrative Assistant, sarrisk@uwplatt.edu, 608-342-7302 1 University Plaza, 201 Ottensman Hall, Platteville, WI 53818

www.uwplatt.edu/chemistry

Raja Annamalai Asst. Professor, Organic annamalaiv@uwplatt.edu 608-342-6091

Brian Barry Asst. Professor, Inorganic barryb@uwplatt.edu 608-342-7377

Soma Chattopadhyay Asst. Professor, Physical chattopadhys@uwplatt.edu 608-342-1662

Charles Cornett Professor, Analytical cornettc@uwplatt.edu 608-342-1658

Kari Frederick Lab Manager frederik@uwplatt.edu 608-342-1467

Molly Fuller Lab Assistant fullermol@uwplatt.edu 608-342-1467

Roger Gurira Lecturer, Analytical gurirar@uwplatt.edu 608-342-7134

James Hamilton Professor, Physical/Nanotechnology hamiltoj@uwplatt.edu 608-342-1670

Qiong (June) Li Professor, Organic (Chair) liq@uwplatt.edu 608-342-1498

Farzana Marni Lecturer marnif@uwplatt.edu 608-342-7376

Bruna Pelucchi Lecturer pelucchib@uwplatt.edu 608-342-1273

Kimberly Pulkrabek Lecturer, Organic pulkrabekk@uwplatt.edu 608-342-7135

Raymond Pugh Asst. Professor, Biochemistry pughr@uwplatt.edu 608-342-6019

Mohammad Rabbani Asst. Professor, Inorganic rabbanim@uwplatt.edu 608-342-7344

Scott Sandholm Lecturer, Physical sandholms@uwplatt.edu 608-342-6090

Steven Steiner Professor, Analytical steiners@uwplatt.edu 608-342-1396

Joseph Wu Assoc. Professor, Analytical wut@uwplatt.edu 608-342-6018

Holly Ziobro Lecturer, Biochemistry ziobroh@uwplatt.edu 608-342-1510

79

UW-River Falls Department of Chemistry and Biotechnology

Janie Huot, Academic Program Assistant, janie.huot@uwrf.edu, 715-425-3523 410 S. Third Street, River Falls, WI 54022

www.uwrf.edu/CHEM

Samuel Alvarado Asst. Professor, Inorganic samuel.alvarado@uwrf.edu 715-425-3871

Dustin Andert Laboratory Manager dustin.andert@uwrf.edu 715-425-4016

Ross Jilk Professor, Biochemistry ross.jilk@uwrf.edu 715-425-4299

Ekaterina Kadnikova Assoc. Lecturer, Organic ekaterina.kadnikova@uwrf.edu 715-425-3654

Mike Kahlow Professor, Physical michael.a.kahlow@uwrf.edu 715-425-4313

Lisa Kroutil Professor, Biochemistry lisa.c.kroutil@uwrf.edu 715-425-4352

Dan Marchand Professor, Medicinal daniel.marchand@uwrf.edu 715-425-4398

Kevin McLaughlin Professor, Physical/Polymer k.w.mclaughlin@uwrf.edu 715-425-4425

Barbara Nielsen Professor, Analytical barb.s.nielsen@uwrf.edu 715-425-4460

Matthew O’Reilly Asst. Professor, Organic matthew.oreilly@uwrf.edu 715-425-3523

Yuliya Paukku Assoc. Lecturer, Physical yuliya.paukku@uwrf.edu 715-425-4842

Karl Peterson Professor, Organic (Chair) karl.peterson@uwrf.edu 715-425-4515

Brent Ristow Assoc. Lecturer, Organic brent.ristow@uwrf.edu 715-425-3523

Jamie Schneider Professor, Chemical Education jamie.schneider@uwrf.edu 715-425-4590

Kurt Torgersen Lecturer, Biochemistry kurt.torgersen@uwrf.edu 715-425-4678

80

UW-Stevens Point Department of Chemistry

Cristina Altobelli, Academic Department Associate, caltobel@uwsp.edu, 715-346-2888 Chemistry.Department@uwsp.edu

www.uwsp.edu/chemistry

Robert C. Badger Professor, Organic rbadger@uwsp.edu 715-346-3700

Nathan Bowling Professor, Organic nbowling@uwsp.edu 715-346-3706

Laura J. Cole Assoc. Professor, Analytical lcole@uwsp.edu 715-346-4302

Tim Corcoran Instructor Tim.Corcoran@uwsp.edu 715-346-3894

Kevin Czerwinski Professor, Organic kczerwin@uwsp.edu 715-346-4154

Jason D'Acchioli Assoc. Professor, Inorganic/Analytical (Chair)

jdacchio@uwsp.edu 715-346-4253

Paul W. Hladky Professor, Physical/Polymer phladky@uwsp.edu 715-346-3711

Amanda Jonsson Asst. Professor, Biochemistry amanda.jonsson@uwsp.edu 715-346-2600

Jim Lawrence Assoc. Professor, Biochemistry jlawrenc@uwsp.edu 715-346-3699

Arin Lemke Lab Instructor alemke@uwsp.edu 715-346-4467

Gary Lueck Lab Instructor glueck@uwsp.edu 715-346-4914

Kathryn McGarry Asst. Professor, Organic kmcgarry@uwsp.edu 715-346-3328

Joseph Mondloch Asst. Professor, Inorganic jmondloc@uwsp.edu 715-346-3715

Gee Pope Instructor gpope@uwsp.edu 715-346-2601

Shannon Riha Asst. Professor, Analytical sriha@uwsp.edu 715-346-2171

Gary J. Shulfer Instructional Specialist, General gshulfer@uwsp.edu 715-346-4138

David Snyder Asst. Professor, Analytical dasnyder@uwsp.edu 715-346-2155

Brent Speetzen Stockroom Manager bspeetze@uwsp.edu 715-346-3759

Erin Speetzen Assoc. Professor, Biophysical espeetze@uwsp.edu 715-346-3258

Dave Szpunar Asst. Professor, Physical dszpunar@uwsp.edu 715-346-2888

Robin Tanke Professor, Organic rtanke@uwsp.edu 715-346-4325

Tony P. Timerman Professor, Biochemistry atimerma@uwsp.edu 715-346-3167

James Tuszka Sr. Electronics Tech jtuszka@uwsp.edu 715-346-4201

81

UW-Stout Department of Chemistry

Tammy Wolf, Department Associate, wolft@uwstout.edu, 715-232-2268 410 10th Ave., Jarvis Hall Science Wing 331A, Menomonie, WI 54751

www.uwstout.edu/chemistry

Jonathan Frisch Asst. Professor frischj@uwstout.edu 715-232-5305

Rebecca Hoeft Laboratory Manager/Chemistry Hygiene Officer

hoeftr@uwstout.edu 715-232-2151

Dmitry Kadnikov Assoc. Professor kadnikovd@uwstout.edu 715-232-4073

Shane Medin Lecturer, General medins@uwstout.edu 715-232-2148

Marcia Miller-Rodeberg Assoc. Professor, Biochemistry miller-rodebergm@uwstout.edu

715-232-2146

Josiah Ray Laboratory Manager rayjo@uwstout.edu 715-232-2292

Matthew Ray Asst. Professor, Polymer/Materials/Nanotech

rayma@uwstout.edu 715-232-2292

Forrest Schultz Professor, Organic/Nanotechnology schultzf@uwstout.edu 715-232-5009

Ana Vande Linde Professor, Analytical/Inorganic (Chair)

vandelindea@uwstout.edu 715-232-3497

82

UW-Superior Natural Sciences Department

Sandra Orr, Academic Department Associate, sorr@uwsuper.edu, 715-394-8322 Barstow Hall 202, Belknap and Catlin, P.O. Box 2000, Superior, WI 54880

www.uwsuper.edu/acaddept/naturalsciences

Nicholas Danz Assoc. Professor, Plant Science (Chair) ndanz@uwsuper.edu 715-394-8161

James Lane Professor, Organic/Biochemistry jlane@uwsuper.edu 715-394-8204

Lorena Rios-Mendoza Assoc. Professor lriosmen@uwsuper.edu 715-394-8205

Michael Waxman Professor, Physical mwaxman@uwsuper.edu 715-394-8261

83

UW-Whitewater Department of Chemistry Julia Rowehl, Academic Department Associate, rowehlj@uww.edu, 262-472-1070

Upham Hall 220, 800 West Main Street, Whitewater, WI 53190 www.uww.edu/cls/chemistry

Steven W. Anderson Professor, Organic andersos@uww.edu 262-472-5121

Jessica Bonjour Asst. Professor menkej@uww.edu 262-472-1088

Catherine Chan Assoc. Professor, Biochemistry chanc@uww.edu 262-472-5133

John Ejnik Assoc. Professor, Analytical (Chair) ejnikj@uww.edu 262-472-1083

Steven Girard Asst. Professor girards@uww.edu 262-472-1096

Marsha Goodell Laboratory Manager I goodellm@uww.edu 262-472-1610

John Grutsch Lecturer grutschj@uww.edu 262-472-5120

Baocheng Han Professor, Analytical/Inorganic hanb@uww.edu 262-472-5122

Paul House Assoc. Professor, Physical/Inorganic housep@uww.edu 262-472-1551

Hepsi Kumpaty Professor, Organic kumpatyh@uww.edu 262-472-1097

Kimberly Naber Lecturer, Analytical naberk@uww.edu 262-472-1245

Hassimi Traore Assoc. Professor, Physical traoreh@uww.edu 262-472-5123

Christopher Veldkamp Assoc. Professor, Biochemistry veldkamc@uww.edu 262-472-5267

84

The 44th Annual UW System Chemistry Faculties Meeting

Past UW System Chemistry Faculties Meetings Host Institutions

1. 1972 River Falls 2. 1973 La Crosse

3. 1974 Madison 4. 1975 Stevens Point

5. 1976 Milwaukee

6. 1977 Oshkosh

7. 1978 Green Bay

8. 1979 Superior 9. 1980 Parkside

10. 1981 Eau Claire

11. 1982 Platteville

12. 1983 Stout

13. 1984 Marathon 14. 1985 River Falls

15. 1986 Madison

16. 1987 La Crosse

17. 1988 Stevens Point

18. 1989 Milwaukee 19. 1990 Green Bay

20. 1991 Oshkosh

21. 1992 Superior

22. 1993 Eau Claire

23. 1994 Parkside

24. 1995 Stout 25. 1996 Platteville

1997 No Meeting 26. 1998 Whitewater

27. 1999 River Falls

28. 2000 La Crosse

29. 2001 Madison

30. 2002 Stevens Point 2003 No Meeting

31. 2004 Milwaukee

32. 2005 Washington

33. 2006 Oshkosh

34. 2007 Green Bay 35. 2008 Eau Claire

36. 2009 Parkside

37. 2010 Platteville

38. 2011 Stout

39. 2012 Whitewater 40. 2013 River Falls

41. 2014 La Crosse

42. 2015 Madison

43. 2016 Stevens Point

44. 2017 Milwaukee

http://uwc.edu/depts/chemistry

86

The 44th Annual UW System Chemistry Faculties Meeting

List of Participants

Name email Institution

Aldstadt, Joe aldstadt@uwm.edu UW-Milwaukee Allen, Lori allen@uwp.edu UW-Parkside

Annamalai, Raja annamalaiv@uwplatt.edu UW-Platteville

Arnold, Alexander arnold2@uwm.edu UW-Milwaukee

Ayoub, Mohamed mohamed.ayoub@uwc.edu UW-Washington County

Badger, Robert rbadger@uwsp.edu UW-Stevens Point Barta (Rossi), Cheri cbarta@chem.wisc.edu UW-Madison

Benko, Anna abenko@uwm.edu UW-Milwaukee

Blecking, Anja blecking@uwm.edu UW-Milwaukee

Chang, Tsun-Mei chang@uwp.edu UW-Parkside

Chattopadhyay, Soma chattopadhys@uwplatt.edu UW-Platteville Chen, Franklin chenf@uwgb.edu UW-Green Bay

Cole, Laura lcole@uwsp.edu UW-Stevens Point Cook, James capncook@uwm.edu UW-Milwaukee

Cornett, Chuck cornettc@uwplatt.edu UW-Platteville

Crawford, Kevin crawfork@uwosh.edu UW-Oshkosh D'Acchioli, Jason jdacchio@uwsp.edu UW-Stevens Point

Dietz, Mark dietzm@uwm.edu UW-Milwaukee Doolittle, Pamela psdoolittle@yahoo.com UW-Madison

Ejnik, John ejnikj@uww.edu UW-Whitewater

Fischer, Abbey abbey.fischer@uwc.edu UW-Barron County Gallagher, Warren wgallagh@uwec.edu UW-Eau Claire

Grutsch, John grutschj@uww.edu UW-Whitewater Gurira, Roger gurirar@uwplatt.edu UW-Platteville

Gutow, Jonathan gutow@uwosh.edu UW-Oshkosh

Guzei, Ilia iguzei@chem.wisc.edu UW-Madison Hamilton, James hamiltoj@uwplatt.edu UW-Platteville

Hladky, Paul phladky@uwsp.edu UW-Stevens Point House, Paul housep@uww.edu UW-Whitewater

Indig, Gil glindig@uwm.edu UW-Milwaukee

Jilk, Ross ross.jilk@uwrf.edu UW-River Falls

Kabrhel, Amy amy.kabrhel@uwc.edu UW-Manitowoc

Kabrhel, James james.kabrhel@uwc.edu UW-Sheboygan

87

Kahlow, Michael michael.a.kahlow@uwrf.edu UW-River Falls Kroutil, Lisa lisa.c.kroutil@uwrf.edu UW-River Falls

Kumpaty, Hephzibah kumpatyh@uww.edu UW-Whitewater

Lamont, Liana lblamont@wisc.edu UW-Madison

Li, June liq@uwplatt.edu UW-Platteville

Liu, Yujuan liuy@uwp.edu UW-Parkside Mann, Francis mannf@uwp.edu UW-Parkside

Marni, Farzana marnif@uwplatt.edu UW-Platteville

McGarry, Kathryn kmcgarry@uwsp.edu UW-Stevens Point

McIntire, Michael mcintirm@uwgb.edu UW-Green Bay

McMahon, Robert mcmahon@chem.wisc.edu UW-Madison Mihalick, Jennifer mihalick@uwosh.edu UW-Oshkosh

Mondloch, Joseph jmondloc@uwsp.edu UW-Stevens Point

Moore, John jwmoore@chem.wisc.edu UW-Madison

Moran, Graham moran@uwm.edu UW-Milwaukee

Murphy, Kristen kmurphy@uwm.edu UW-Milwaukee Naber, Kimberly naberk@uww.edu UW-Whitewater

Pacheco, Andy apacheco@uwm.edu UW-Milwaukee Pelucchi-Addison, Bruna pelucchib@uwplatt.edu UW-Platteville

Peng, Xioahua pengx@uwm.edu UW-Milwaukee Petering, David petering@uwm.edu UW-Milwaukee

Peterson, Karl karl.peterson@uwrf.edu UW-River Falls

Pugh, Raymond pughr@uwplatt.edu UW-Platteville Pugh, Samantha pughs@uwplatt.edu UW-Platteville

Rabbani, Mohammad rabbanim@uwplatt.edu UW-Platteville Riha, Shannon sriha@uwsp.edu UW-Stevens Point

Sauer, Daryl sauer@uwp.edu UW-Parkside

Schneider, Jamie jamie.schneider@uwrf.edu UW-River Falls Schwabacher, Alan awschwab@uwm.edu UW-Milwaukee

Sibert, Edwin elsibert@wisc.edu UW-Madison Silvaggi, Nicholas silvaggi@uwm.edu UW-Milwaukee

Snyder, Dave dave.snyder@uwsp.edu UW-Stevens Point

Stafford, Douglas dcstaff@uwm.edu UW-Milwaukee Tanke, Robin rtanke@uwsp.edu UW-Stevens Point

Timerman, Tony atimerma@uwsp.edu UW-Stevens Point Vande Linde, Ana vandelindea@uwstout.edu UW-Stout

Wacholtz, William wacholtz@uwosh.edu UW-Oshkosh

Woehl, Jorg woehl@uwm.edu UW-Milwaukee Yang, Thao yangt@uwec.edu UW-Eau Claire

Zhu, Lingchao lzhu@chem.wisc.edu UW-Madison

Zorn, Michael zornm@uwgb.edu UW-Green Bay

88

89

90

91

NOTES

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