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Chapter 8: Antioxidant Activity
178 Ph. D. Thesis: Miss Sandhya V. Rodge, 2015
ANTIOXIDANT ACTIVITY
The antioxidant activity of selected medicinal plants was performed at Chemistry
Research Laboratory, Deogiri College, Aurangabad.
8.1 Preparation of sample solution
About 0.10 g of dry plant powder was accurately weighed in a stopper tube. In
stopper tube, 10.0 ml of aqueous methanol (40%) was added and incubated in a incubator
for about 24 hrs. The contents of the tube were filtered through Whatman filter paper No.
41 (Merck, India). The filtrate was used for further analysis.
8.2 DPPH radical scavenging assay
DPPH (2, 2-dipheny l-2- picrylhydrazyl) radical scavenging assay was carried out as
per reported method with some modifications Blois (1958) and Roberta, et al., (2006).
About 1 ml of test solution was added to equal quantity of 0.1 mM solution of DPPH in
ethanol. After 20 min of incubation at room temperature, the DPPH reduction was
measured by reading the absorbance at 517 nm. Ascorbic acid (1mM) was used as
reference compound and it has 51.33 ± 1.02 % of DPPH antioxidant assay.
Sr. No Plant name DPPH antioxidant assay (%)
1 Cucumis setosus fruit 59.66 ± 1.52
2 Lagenaria siceraria fruit 23.60 ± 1.41
3 Trichosanthes tricuspidata fruit 65.00 ± 1.00
4 Coccinia grandis fruit 72 .14 ± 0.52
5 Diplocyclos palmatus fruit 60.33 ± 0.25
6 Cucumis setosus leaf 29.26 ± 0.64
7 Trichosanthes tricuspidata leaf 54.00 ± 1.47
8 Coccinia grandis leaf 18.40 ± 0.78
9 Diplocyclos palmatus leaf 22.33 ± 0.49
10 Lagenaria siceraria leaf 14.85 ± 0.73
Standard Ascorbic Acid 51.33 ± 1.02
Table No 128: Antioxidant activity of selected medicinal plants
Chapter 8: Antioxidant Activity
179 Ph. D. Thesis: Miss Sandhya V. Rodge, 2015
8.3.1 Antioxidant activity of Cucumis setosus fruit
The antioxidant activity of methanol fruit extract of C. setosus was examined by
comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH
antioxidant assay percentage is 59.66 ± 1.52.
8.3.2 Antioxidant activity of Lagenaria siceraria fruit
The antioxidant activity of methanol fruit extract of Lagenaria siceraria was
examined by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 23.60 ± 1.41
8.3.3 Antioxidant activity of Trichosanthes tricuspidata fruit
The antioxidant activity of methanol fruit extract of Trichosanthes tricuspidata
was examined by comparing it to the activity of standard antioxidant such as Ascorbic
acid. The DPPH antioxidant assay percentage is 65.00 ± 1.00
8.3.4 Antioxidant activity of Coccinia grandis fruit
The antioxidant activity of methanol fruit extract of Coccinia grandis was examined
by comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH
antioxidant assay percentage is 72 .14 ± 0.52
8.3.5 Antioxidant activity of Diplocyclos palmatus fruit
The antioxidant activity of methanol fruit extract of Diplocyclos palmatus was examined
by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 60.33 ± 0.25
8.3.6 Antioxidant activity of Cucumis setosus leaf
The antioxidant activity of methanol leaf extract of Cucumis setosus was examined by
comparing it to the activity of standard antioxidant such as Ascorbic acid. The DPPH
antioxidant assay percentage is 29.26 ± 0.64.
Chapter 8: Antioxidant Activity
180 Ph. D. Thesis: Miss Sandhya V. Rodge, 2015
8.3.7 Antioxidant activity of Trichosanthes tricuspidata leaf
The antioxidant activity of methanol leaf extract of Trichosanthes tricuspidata was
examined by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 54.00 ± 1.47
8.3.8 Antioxidant activity of Coccinia grandis leaf
The antioxidant activity of methanol leaf extract of Coccinia grandis was
examined by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 18.40 ± 0.78
8.3.9 Antioxidant activity of Diplocyclos palmatus leaf
The antioxidant activity of methanol leaf extract of Diplocyclos palmatus was
examined by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 22.33 ± 0.49
8.3.10 Antioxidant activity of Lagenaria siceraria leaf
The antioxidant activity of methanol leaf extract of Lagenaria siceraria was
examined by comparing it to the activity of standard antioxidant such as Ascorbic acid.
The DPPH antioxidant assay percentage is 14.85 ± 0.73
The DPPH free radical scavenging activity of standard reference ascorbic acid was
51.33 ± 1.02. In present study the DPPH free radical scavenging activity of fruit was
more than that of reference standard ascorbic acid.
Antioxidant analysis of present study reveals that the maximum DPPH antioxidant
assay percentage was observed in Coccinia grandis fruit 72.14 ± 0.52, Trichosanthes
tricuspidata fruit 65.00 ± 1.00, Diplocyclos palmatus fruit 60.33 ± 0.25 and Cucumis
setosus fruit 59.66 ± 1.52 which was more than that of standard ascorbic acid.
The Trichosanthes tricuspidata leaf has DPPH free radical scavenging activity 54.00
± 1.47 which also more than that of standard ascorbic acid. The results were dedicated in
Table No 128 and Graph Nos 11& 12).
Chapter 8: Antioxidant Activity
181 Ph. D. Thesis: Miss Sandhya V. Rodge, 2015
0
10
20
30
40
50
60
Cucumis setosus
Trichosanthes tricuspidata
Coccinia grandis
Diplocyclos palmatu
Lagenaria siceraria
Plant Part of leaf
Graph No: 11 Antioxidant activity of leaf of selected plants
Plant Part Ascorbic Acid
Chapter 8: Antioxidant Activity
182 Ph. D. Thesis: Miss Sandhya V. Rodge, 2015
0
10
20
30
40
50
60
70
80
Cucumis setosus
Lagenaria siceraria
Trichosanthes tricuspidata
Coccinia grandis
Diplocyclos palmatus
Plant Part of fruit
Graph No :12 Antioxidant activity of fruit of selected plants
Plant Part Ascorbic Acid