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APPLIED MICROBIOLOGYVOLUME 28 * NUMBER 6 * DECEMBER 1974
EDITORIAL BOARD
MARVIN P. BRYANT, Editor-in-Chief (1976)University of Illinois, Urbana
ALBERT BALOWS, Editor (1975)Center for Disease Control,
Atlanta, Ga.
R. H. DEIBEL, Editor (1976)University of Wisconsin, Madison
A. L. DEMAIN, Editor (1976)Massachusetts Institute of Technology, Cambridge
NATHALIE J. SCHMIDT, Editor (1975)California State Department of
Public Health, BerkeleyJAMES M. TIEDJE, Editor (1979)
Michigan State University,East Lansing
Bernard J. Abbott (1977)Libero Ajello (1976)Milton J. Allison (1974)Richard Bartha (1976)Martha D. Berliner (1977)Jerome Birnbaum (1976)Lee A. Bulla, Jr. (1977)Francis F. Busta (1975)William R. Chesbro (1976)Alex Ciegler (1974)Rita R. Colwell (1974)Richard A. Consigli (1976)Charles Cooney (1976)Hugo L. David (1975)Jacob A. Donkersloot (1976)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1975)Richard Elander (1976)Bernice England (1977)Martin S. Favero (1975)John C. Feeley (1974)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Morris Fishbein (1976)Heinz G. Floss (1977)Edwin E. Geldreich (1976)S. E. Gilliland (1974)John M. Goepfert (1976)
Charles T. Goodhue (1977)Morris A. Gordon (1975)Robert D. Hamilton (1976)Richard S. Hanson (1975)James L. Hardy (1975)Paul A. Hartman (1974)Melvin T. Hatch (1975)Clifford W. Hesseltine (1974)Lillian V. Holdeman (1976)John J. Iandolo (1974)M. B. Ingle (1976)Henry D. Isenberg (1974)Holger W. Jannasch (1975)Donald E. Jensen (1977)S. S. Kalter (1975)Edward Katz (1976)Lloyd L. Kempe (1976)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Barbara D. Lago (1977)Hubert Lechevalier (1976)Paul Lemke (1976)John Liston (1974)Warren Litsky (1975)Robert Mah (1974)William J. Martin (1975)Joseph L. Melnick (1976)William I. Metzger (1974)Thomas L. Miller (1976)
David Paretsky (1976)W. J. Payne (1976)D. Perlman (1974)David Pramer (1974)Antonio H. Romano (1977)E. L. Schmidt (1974)Oldrich K. Sebek (1974)Warren S. Silver (1976)Anthony J. Sinskey (1976)Louis DS. Smith (1975)Peter B. Smith (1976)S. S. Socransky (1975)Alex C. Sonnenwirth (1975)Mortimer P. Starr (1975)Guenther Stotzky (1974)Robert B. Tompkin (1976)Marvin Turck (1974)Carl Vanderzant (1976)Claude Vezina (1976)D. I. C. Wang (1975)Lewis W. Wannamaker (1975)John A. Washington H (1975)Robert E. Weaver (1975)Sydney J. Webb (1975)Fred D. Williams (1977)R. P. Williams (1975)Benjamin J. Wilson (1975)G. N. Wogan (1974)Alan G. Wolin (1974)
ROBERT A. DAY, Managing EditorGISELLA POLLACK, Director of Editorial Services
CHERYL A. CROSS, Production Editor1913 I St., N.W., Washington D.C. 20006
EX OFFICIOPHILIPP GERHARDT, President (1974-1975) HELEN R. WHITELEY, Vice-President (1974-1975)J. MEHSEN JOSEPH, Secretary
Applied Microbiology, a publication of the American Societyfor Microbiology, 1913 I St., N.W., Washington, D.C. 20006, isdevoted to the advancement and dissemination of applied knowl-edge as well as ecological knowledge, both applied and funda-mental, concerning microorganisms. Applied Microbiology is pub-lished monthly, two volumes per year. The subscription price is$60 (foreign, $61) per year. Single copies are $6.00 (foreign,$6.25). Members of the American Society for Microbiology mayreceive Applied Microbiology as part of their dues. Correspond-ence relating to subscriptions, nonreceipt of journals, reprints, de-fective copies, availability of back issues, and lost or late proofsshould be directed to the ASM Publications Office, 1913 I St.,
T. J. CARSKI, TreasurerN.W., Washington, D.C. 20006 (area 202 833-9416). Correspond-ence from ASM members relating to membership dues, membersubscriptions, changes of address, incorrect address, incorrectjournals, etc., should be directed to the Executive Secretary,American Society for Microbiology, 1913 I St., N.W., Washington,D.C. 20006. Published monthly by the ASM at 1913 I St., N.W.,Washington, D.C. 20006.
Second-class postage paid at Washington, D.C. 20006, andat additional mailing offices. Made in the United States ofAmerica.Copyright © 1975, American Society for Microbiology.All Rights Reserved.
Author IndexAlexander, M., 1084Applegate, Kenneth L., 1027Arnold, Wilfred N., 1047Arthur, L. O., 1040Ayres, J. C., 1094
Battat, E., 901, 906Bergdoll, Merlin, 946Berryhill, D. L., 1030Biano, S. A., 992Boyd, Karen S., 1047Brown, M. W., 1069Bryant, M. P., 1018
Caldwell, Daniel It., 1088Carpenter, J. A., 1063Casida, L. E., Jr., 951Chang, Te Wen, 992Chipley, John II., 1027Cho, John J., 987Colwell, R. IR., 977, 982, 1009Crowley, Julia, 943
Daniels, J. B., 992Dawes, E. A., 999Deal, P. H., 1066DiCuollo, C. J., 995Dominik, Joseph W., 929Dreyfuss, Mark S., 1027
Edwards, E. A., 972Elliot, J. G., 1063Evans, James B., 1086
Fare, L. It., 995Fine, D. L., 1040
Gaafar, Hassan, 1073Garrison, Robert G., 1047Gilliland, S. E., 1090Glassman, Armand B., 943Goldberg, I., 906Gould, Sara, 946
Green, Sylvia K., 987
Haines, J. R., 1084Hamann, A. C., 1076Hamdy, M. K., 1063Hart, A., 1069Hechemy, Karim, 1073Herbeck, Janice L., 1018Hillman, E. A., 1040Hutchinson, Harriet D., 935
Jurtshuk, Peter, 1079
Kaneko, Tatsuo, 1009Klein, F., 1040Koehler, P. E., 1094Kominos, Spyros D., 987Kunsman, Joseph E., 1088
Larson, Edgar W., 929Larson, G. L., 972Liu, Kang-chien, 951Liu, Paul I., 943
Mabee, Michael S., 1027McDorman, Donald J., 968Mack, H. M., 1066Mallette, M. F., 999Mateles, RI. I., 901, 906Mayner, Ronald E., 968Meyer, Barbara C., 968Miller, J. A., 995Milligan, Thomas W., 1079Moon, Nancy J., 1076Mulkins-Phillips, G. J., 915Munoz, Elaine F., 960
Ohishi, Iwao, 923
Pagano, J. F., 995Parkman, Paul D., 968Peleg, Ben-Ami, 1055
Plowman, J. K., 1040
Reece, Paul, 999Reinbold, G. W., 1076Richmond, Shirley, 912Riley, P. S., 1071Robbins, Ruth, 946Ron, Nily, 1055
Sakaguchi, Genji, 923Saldick, Jerome, 1004Schipper, I. A., 1030Schroth, Milton N., 987Schwarz, J. It., 977, 982Schwert, David P., 1082Silverman, Melvin P., 960Sompolinsky, David, 912Souza, K. A., 1066Speck, M. L., 1090Sroka, Jean, 1073Stevens, Roy, 1073Stewart, James E., 915Sullivan, James D., Jr., 1023Switzer, R. E., 1086
Tschider, S. R., 1030Turnbill, C. E., 1066
Valenta, J. R., 995Vitanza-Jack, Vilma B., 987
Walker, J. D., 982Watson, Stanley W., 1023Weaver, It. E., 1071White, James P., 1082Wiebe, W. J., 1033Wu, M. T., 1094
Yetka, J. E., 1033Young, Harold W., 929
Ziegler, I)onald W., 935
Acknowledgment
The Editors acknowledge with appreciation the able assistance and guidance generously pro-vided by the following invited, special reviewers during 1974:
Bernard J. AbbottIrving AbrahamsDonald AhearnMartin AlexanderS. D. AllenH. L. BachrachEdward F. BaerSamuel BaronA. L. BarronEverett BenekeMerlin S. BergdollMartha D. BerlinerMarjorie BissettNilambar BiswalGabriel BittonHoward Bladen, Jr.Donna J. BlazevicPaul M. BorickThomas L. BottVirginia C. BowenWinston J. BrillThomas D. BrockF. BrownGene M. BrownRobert D. BrunerThomas BuchananSonja M. BuckleyLee A. Bulla, Jr.Robert H. BussellErcole Canale-ParolaL. E. CarmichaelHelen CaseyL. E. Casida, Jr.Richard CastenholzT. H. CateB. Wesley CatlinW. A. CauseyW. B. CherryTom D. Y. ChinVincent P. CirilloAnita J. ClemetsonDean 0. CliverWallace A. Clyde, Jr.Michael ColeCharles D. CoxNatalie CremerJoan CrickE. V. CrisanG. K. DarlandEugene A. DelwicheW. E. DeWitt
Frank J. DixonRichard DixonJacob A. DonkerslootGordon DreesmanCharles L. DuncanHenry L. EhrlichHerman C. Ellinghausen, Jr.Richard EmmonsBeatrice EnglandJerald C. EnsignJ. B. EvansRichard R. FacklamJ. J. Farmer IIIJames J. FerrisR. A. FinkelsteinRobert K. FinnHeinz G. FlossDennis D. FochtJ. W. FosterLloyd R. FrederickLorraine FriedmanPaul GerberPhillip GerhardtRonald GibbonsMillicent GoldschmidtRichard E. GoldstrandDwight GordonRuth GordonSteve A. GouldenMichael GrossmanL. B. GuzeAshley HaaseAdeline J. HackettG. J. HageageL. D. HaleyR. P. HansonJanet W. HartleyW. J. Hausler, Jr.Leonard HayflickCharles W. HendricksGertrude HenleS. D. HenricksenG. J. HermannJ. C. HierholzerRonald D. HinsdillF. B. HollingerKing HolmesHope E. HoppsE. C. HorningDennis P. H. HsiehRudolph Hugh
Nino F. InsalataDonald E. JensenR. C. JohnsonW. L. JonesElliot JuniIrving G. KaganAlbert Z. KapikianA. G. KarlsonDavid T. KarzonLeo KaufmanDonald A. KautterD. S. Kellogg, Jr.Donald A. KleinG. C. KleinM. J. KlugAlexis J. KniazeffPatrick J. KonkelLawrence R. KoupalS. J. KrausR. M. KrauseC. M. KuninBarbara D. LagoR. C. LancefieldM. P. LechevalierJ. LederbergG. E. LewisJ. M. LindseyDon C. MackelJ. D. MacLowreyHoward MalmstadtMary MandelsRobert E. MarquisJ. E. MartinW. McCormackGordon A. McFetersBetty E. McGrewR. M. McKinneyD. R. MertensRichard C. MeyerRalph MitchellC. J. MirochaDan H. MooreG. K. MorrisC. W. MossG. N. MowatA. J. NahmiasJohn D. NelsonWalter A. Nelson-ReesH. C. NeuRobert F. NystromZ. John Ordal
Richard A. OrmsbeeStephen OroszlanLyndon OshiroBruce OstlerJohn C. ParkerJack L. PateHerbert PerkinsJohn T. PfefferNorbert PfennigJ. C. PickettBertie PittmanSteven PorterJ. R. PostgateRobert PurcellT. J. QuanWilliam E. RawlsC. A. ReddyE. T. ReeseL. B. RellerH. Y. ReynoldsJohn L. RiggsP. S. RileyWilliam S. RobinsonMorrison RogosaAntonio H. RomanoPhilip K. RussellRobert S. SaffermanJoseph SalanitroWilliam E. SandineR. W. Scales
Julius SchachterPriscilla SchafferEdward J. SchantzWilliam F. SchererJack H. SchiebleRamon SeidlerJohn C. SherrisV. A. SilcoxPeter SkaliyRalph SlepeckyEugene B. SmallH. L. Smith, Jr.Paul F. SmithRodney F. SmithPeter H. A. SneathMark D. SobseyMarion D. SocolofskyWilliam SpanglerRex S. SpendloveDon F. SplittstoeserJames T. StaleyW. E. StammEric StanbridgeJ. L. StokesHenry 0. Stone, Jr.Hiroshi SugiyamaC. R. SulzerR. D. SwisherR. A. TerryB. M. Thomason
Clyde ThornsberryEdwin TraismanHugh L. TrenkHenry M. TsuchiyaJon TuttleRobert L. UffenLarry N. VanderhoefGail E. VandermollenPeter Van SoestAlexander von GraevenitzCharles H. WalkinshawK. W. WallsMichael WeberThomas H. WellerD. W. S. WestlakeBruce WetzelDavid C. WhitePaul J. WiesnerT. J. WiktorHazel W. WilkinsonFred D. WilliamsJ. B. WilsonKenneth E. WolfP. C. WolkMeyer J. WolinSan-Pin WongR. J. WoodDavid S. YohnV. M. YoungClaude E. Zobell
APPLIED MICROBIOLOGY
INSTRUCTIONS TO AUTHORS
Correspondence. Submit manuscripts in duplicate(original and one carbon) to ASM Publications Office,1913 I St., N.W., Washington, D.C. 20006.General policy. Any manuscript submitted must be
a report of unpublished original research, which is notbseing considered for publication elsewhere. Eachmanuscript should present the results of an inde-pendent, cohesive study; "series" papers are discour-aged.The "editorial style" of Applied Microbiology essen-
tially follows the CBE Style Manual (3rd ed., AIBS,1972). Genetics symbols should essentially follow therecommendations of Demerec et al. (Genetics 54:61,1966). Biochemical abbreviations and nomenclatureshould essentially follow "Biochemical Nomencla-ture" in Handbook of Biochemistry (2nd ed., 1970, H.A. Sober, ed., The Chemical Rubber Co., Cleveland,p. A4-A24). Normally, abbreviations (except those ofstandard units of measurement and symbols of theelements) should be defined and introduced paren-thetically at first use in the text. Enzyme activity shouldhe expressed in terms of international units (EnzymeNomenclature, Elsevier Publishing Co., 1972), and theEC number should be given parenthetically at first usein the text. In expressing lengths, weights, and vol-umes, the prefixes nano (n) and pico (p) should beused instead of millimicro (mA) and micromicro (gut).Express lengths in nanometers (nm; 10-9 m) or in mi-crometers (,Am; 10-6 m) instead of millimicrons (mg;10- m), microns (,s; 10-6 m), or Angstroms (A;10-1i m). Express parts per million (ppm) as micro-grams per milliliter (,gg/ml), micrograms per gram(;&g/g), or microliters per liter (,dliters/liter), as ap-propriate. In general, measurements should be ex-pressed in terms of standard international metric units.Applied Microbiology reserves the privilege of editingmanuscripts to make them conform with the adoptedstyle.Form of manuscript. All parts of the manuscript
should be typed double-space or, preferably, triple-space. Most manuscripts can and should be dividedinto the following sections: Abstract, Introduction,Materials and Methods, Results, Discussion, Acknowl-edgements, and Literature Cited. Only one weight ofheading (paragraph lead-in) should be used withineach section.
Title. A single statement of the subject of a paperis preferred to a main title-subtitle arrangement. Ashort version of the title (no more than 46 charactersand spaces) should be supplied for use as a runninghead.
Abstract. An Abstract appears at the beginning ofeach paper. The Abstract should not exceed 250words.
Literature Cited. In the text, references are cited bynumber. The Literature Cited section should be typedin alphabetical order, by first author, and numbered.Names of journals are abbreviated according toBibliographic Guide for Editors & Authors (AmericanChemical Society, 1974). Literature citations shouldbe restricted to closely pertinent papers. Citations ofabstracts, theses, "unpublished data," "personal com-
munications," and "in press" will not be accepted inthe Literature Cited.
Tables. Each table should be typed on a separatepage. The data should be arranged so that columns oflike material read down, not across. The headingsshould be sufficiently clear so that the meaning of thedata will be understandable without reference to thetext. Explanatory footnotes are permitted, but detaileddescriptions of the experiments are not. The materialsand methods used to gain the data should properlyremain in the section of that name.
Figures. A complete set of figures, preferably glossyphotographs, should accompany each of the twocopies of the manuscript. Graphs (submit as photo-graphs) should be finished drawings not needingfurther artwork or type-setting. Absolutely no partof a graph should be typewritten (except the legend,which should be typed on a separate page). Each fig-ure should be numbered and should include the nameof the author, either in the margin or on the back(marked lightly with a soft pencil). All letteringshould be done with a lettering set. Most graphswill be reduced to 6.6 cm in width, and all elementsin the drawing should be prepared to withstand thisreduction. The legend of the figure should provideenough information so that the figure is understand-able without reference to the text. Experimental de-tails from Materials and Methods should not berepeated in figure legends.Nomenclature of microorganisms. In general, the
nomenclature for bacteria presented in Bergey'sManual of Determinative Bacteriology (8th ed., 1974)is used. If an author challenges this nomenclature,his own judgment will be followed, but the name inBergey's Manual should follow in parentheses thefirst time the name is used in the text and in theAbstract. Papers which include extensive taxonomicmaterial (e.g., description of new taxa) will not bepublished in this journal. The proper place for pub-lication of taxonomic material is the InternationalJournal of Systematic Bacteriology (IJSB), which ispublished by the ASM for the International Associa-tion of Microbiological Societies. If the main thrustof such a paper is not taxonomy, the paper should bedivided, the taxonomy portion being submitted toIJSB and the other portion to this journal.
Notes. The accepted form for Notes is somewhatdifferent from the foregoing. Contributors shouldconsult a recent issue of Applied Microbiology forstyle. Notes should not exceed 500 words. The ab-stract should not exceed 25 words.
Copyright. Once a paper has been published inApplied Microbiology, which is a copyrighted publica-tion, the legal ownership of all parts of the paper, in-cluding the illustrations, has passed from the authorto ASM. If the same author, or any author, wishes torepublish material previously published in AppliedMicrobiology, he must first receive written permissionfrom ASM.
Reprints. Reprints (in multiples of 100) may be pur-chased by contributors. A table showing the cost ofreprints, and an order form, will be sent with the proof.
AUTHOR INDEX
VOLUME 28
Abbott, Bernard J., 58Adams, D. M., 793Adams, James N., 423Aemura, Akinori, 366Ahearn, D. G., 723Ahmed, Aftab, 326Ajello, L., 218Alexander, A. D., 505Alexander, M., 248, 1084Allen, Alfred M., 600Amundson, Stanley, 298Anderson, Roger L., 778Anthony, Darrell W., 885Anthony, Nathaniel C., 501Antoine, A. D., 41Applegate, Kenneth L., 1027Appleman, M. D., 460Arnold, Wilfred N., 1047Arthur, L. 0., 1040Ash, Ronald J., 26Ayres, J. C., 1094
Baccash, P., 417Bacchin, Paolo, 737Bachrach, Uriel, 169Bachrach, Zelilah, 169Back, Arthur F., 392, 400Baer, Herman, 618Baker, F. L., 708Balamuth, William, 64Baldwin, Jack N., 86Baldwin, K. A., 342Balfour, Henry H., Jr., 807Bancroft, William H., 600Band, R. Neal, 64Barach, J. T., 793Barksdale, Alma W., 544Bartlett, K. H., 35Battat, E., 901, 906Bell, J. B., 474Benjamin, Denis R., 47, 568Bergdoll, Merlin, 946Bergeron, G., 717Berkowitz, David M., 651Berryhill, D. L., 1030Beuscher, Davis B., 288Biano, S. A., 992Bibel, David, 271Bigner, Darell D., 156Bilimoria, S. L., 133Biswal, Nilambar, 239Blake, I. G., 439Blasi, Dianna, 112Blazevic, Donna J., 22, 759Blosser, Russell O., 672Bohlool, B. B., 11Bolin, H., 262
Bolin, Richard C., 320Bond, Walter W., 406, 822Border, Maryon M., 730Boyd, Karen S., 1047Braswell, J. R., 328Braude, Abraham I., 298Brendle, J. J., 505Brennan, K., 668Britt, Lois, 320Britt, Michael R., 95Brock, T. D., 11Brown, Bruce L., 518Brown, L. R., 753Brown, M. W., 1069Brown, R. W., 768Bryant, Marvin. P, 251, 594,
1018Buchanan, Jack R., 589Buckley, Sonja M., 319Buddemeyer, Edward U., 177Bulla, Lee A., Jr., 124, 129Bullock, G. L., 811Burke, John P., 95Butler, M., 530
Cabelli, V. J., 515Cagle, Gerald D., 312Caldwell, Daniel R., 456, 1088Calisher, Charles H., 881Callahan, Lynn T., III, 326Camargo, Edwaldo E., 452Capone, J. J., 498Carbonero, Pilar, 165Carpenter, David F., 628Carpenter, J. A., 1063Carson, Loretta A., 822Casals, Jordi, 319Casida, L. E., Jr., 951Caslow, M., 435Cassel, William A., 726Castellano, Carmine, 383Causey, William A., 193Champion, R. A., 723Chang, Te Wen, 992Chang-Chien, Mei-Mon, 456Chen, P. K., 811Chen, T. C., 679, 681Chen, Y-K., 892Chibata, Ichiro, 117Chipley, John R., 1027Cho, C. T., 557Cho, Hyun J., 897Cho, John J., 987Christiansen, L. N., 262Clark, Maxine J., 641Cliver, Dean O., 895Coleman, Philip H., 351
i
Coltorti, Emilio A., 1Colwell, R. R., 185, 977,982,1009Cook, R. A., 154Cook, T. M., 579Cook, W. L., 723Coriell, Lewis L., 647Covert, Scott V., 374Crowley, Julia, 943Curran, B. J., 276Curran, Donald, 655Curtis, Roy W., 362
Daghfous, Toufique, 688Dalland, Eli, 856Daniels, J. B., 992David, Hugo L., 696David, Thomas J., 406Davis, Charles E., 298Dawes, E. A., 999Dawson, Chandler R., 688Dayton, Sandra L., 112Deal, P. H., 1066De Jonckheere, J., 159Demain, Arnold L., 265de St. Jeor, S. C., 145Detroy, R. W., 733DiCuollo, C. J., 995Dominik, Joseph W., 929Dreesman, Gordon, 239Dreyfuss, Mark S., 1027Dugan, D. L., 205Duncan, C. L., 815Duncan, I. B. R., 534Dutka, B. J., 474Duxbury, J. M., 248Dyckman, John D., 431
Edelman, Charlene K., 807Edwards, E. A., 972Efthymiou, C. J., 411, 417Ehrlich, H. L., 785El Kholy, Aziz, 836Elliot, J. G., 1063Ellner, P. D., 435, 513, 668Epstein, D. S., 417Evans, James B., 1086Evans, Ralph L., 288
Fare, L. R., 995Favero, Martin C., 406Favero, Martin S., 822Federici, Brian A., 885Feng, K. K., 557Fernandez De Caleya, R., 165Fields, M. L., 638Findley, J., 460Fine, D. L., 1040
AUTHOR INDEX
Finn, R. K., 181Firehammer, B. D., 730Fischer, J. R., 515Floyd, Julia, 91Forghani, Bagher, 661Fortlage, Robert J., 589Francis, A. J., 248Fredrickson, A. G., 831Fujimura, Setsuo, 5
Gaafar, H. A., 306Gaafar, Hassan, 1073Gabridge, Michael G., 774Galyan, Elizabeth L., 456Garcia, W. J., 763Garrison, Robert G., 1047Gaustad, J. W., 748Ghiorse, W. C., 785Gilden, R. V., 100Gilliland, S. E., 1090Gjertsen, P., 31Glassman, Armand B., 943Goldberg, I., 906Goode, Ronald L., 86Gould, Sara, 946Gray, Samuel B., Jr., 320Green, James H., 320Green, Sylvia K., 987
Haferkamp, Otto, 867Haines, J. R., 1084Hald, B., 31Hamann, A. C., 1076Hamdy, M. K., 1063Hankin, Lester, 523Hansen, S. L., 798Hanson, Robert P., 802Harada, Tokuya, 336Hardesty, D. R., 798Harding, Gherry, 802Harrell, William K., 320Harris, Robert E., 351Hart, A., 1069Harwig, Joost, 892Hatcher, H. J., 748Hayashi, Takayoshi T. A., 5Hazard, Edwin I., 885Hechemy, K., 306Hechemy, Karim, 1073Hendricks, Charles W., 572Herbeck, Janice L., 1018Hernandez-Lucas, C., 165Herrmann, John E., 895Hersch, Paul A., 582Heymer, Berno, 867Hill, William F., Jr., 501Hillman, E. A., 1040Hinds, D. B., 521Ho, Monto, 651Hoadley, A. W., 328Hoff, John C., 501Hofstad, T., 856Holdeman, L. V., 594Holder, Ian A., 465
Hollis, D. G., 355Hoshiwara, Isao, 688Howard, A. T., 521Hsieh, D. P. H., 212Hsieh, Dennis P. H., 52Hudson, Bruce W., 323Huebner, Robert J., 614Huisingh, Joellen, 489Huq, Imbadul, 586Hutchinson, Harriet D., 935
Iandolo, John J., 510Ichishima, Eiji, 742Irwin, Gilbert R., 600
Jackson, M. J., 474Jaeger, June, 271Jakubowski, Walter, 501Janardan, Konanur G., 605Johnson, Erious, 513Jones, Henry E., 271Joseph, S. W., 411Jurtshuk, Peter, 1079
Kakimoto, Toshio, 117Kalmakoff, J., 133Kaneko, Tatsuo, 1009Kasatiya, S. S., 317Kaufman, Leo, 641Kautter, Donald A., 722Kelloff, Gary J., 614Kennedy, Barry P. C., 892Kiehn, T. E., 435, 668King, Robert D., 271Kirk, B. E., 17Klein, F., 1040Knowles, R., 608Knudsen, Richard C., 326Ko, Swan Djien, 347Koehler, P. E., 1094Kominos, Spyros D., 987Konowalchuk, J., 717Kostenbader, Kenneth D., Jr.,895
Krause, Richard M., 836Krogh, P., 31Kunsman, Joseph E., 1088Kurtzman, C. P., 708
Labombardi, V. J., 417Lafleur, Lucette, 469Lambe, Dwight W., Jr., 561Lambert, M. A., 80Lambert, N. G., 317Lambrow, M., 763Langford, Edgar V., 897Larson, Edgar W., 929Larson, G. L., 972Larson, Steven M., 452La Scolea, Leonard J., Jr., 70Laskin, Allen I., 58Lasure, Linda L., 544Lazaroff, Norman, 872LeBlanc, Armand J., 722
Lee, Inn Hee, 831Lee, P. P. Chen, 638Lee, Wie-Shing, 651Lennette, Edwin H., 661Levin, M. A., 515Levin, R. E., 679, 681Lillehoj, E. B., 763Lilly, Timothy, Jr., 722Lin, Shundar, 288Lindell, Kermit F., 406Liston, John, 655Liu, Kang-chien, 951Liu, Paul I., 943Long, Maxine C., 605Lund, Marlys E., 22Lynt, Richard K., 722
Mabee, Michael S., 1027McCarthy, C. M., 151McCaskill, Kathy M., 723McComb, Dorothy E., 727McCoy, C. J., 58McDorman, Donald J., 968McDuff, C. R., 748McGarrity, Gerald J., 647McGinnis, M. R., 218Mack, H. M., 1066McKay, L. L., 342Mackowiak, P., 586MacLowry, James D., 691Macmillan, Bruce G., 465McNeill, J. J., 489Mahony, D. E., 172Maker, Myron D., 303Maki, Dennis G., 778Maki, Leroy R., 456Makino, Toshikazu, 5Mallavia, Louis, 713Mallette, M. F., 999Maness, Kathryn S. C., 881Martin, M. L., 481Martin, Mary L., 713Masurekar, Prakash S., 265Matches, Jack R., 655Mateles, R. I., 901, 906Matrone, G., 489Matsen, John M., 22, 672May, Deborah Charsha, 145Mayner, Ronald E., 968Melnick, Joseph L., 232, 861Merkal, R. S., 276Merricks, D. L., 106Merwin, W. H., 80Mesadi, Mohammed, 688Meyer, Barbara C., 968Michaud, G. L., 223Miller, J. A., 995Milligan, Thomas W., 1079Moffatt, Craig M., 95Molskness, T. A., 753Moncada, Luz H., 138Moon, Nancy J., 1076Moore, W. E. C., 594Morahan, Page S., 351
* *
APPL. MICROBIOL.
AUTHOR INDEX
Moss, C. Wayne, 80Muirhead, P. A., 439Mulkins-Phillips, G. J., 547,915Munnecke, Douglas M., 212Munoz, Elaine F., 507, 960Myers, B. M., 798Myers, Donald M., 1Myers, L. L., 730Myken, F., 31
Nagle, Stanley C., 518Nagodawithana, Tilak W., 383Nathan, Paul, 465Nawar, W. W., 679Nelson, David R., 258Neufeld, R., 608Nickerson, Kenneth W., 124,
129Niilo, L., 889Nishimura, Noriyuki, 117
Oberhofer, Thomas R., 720Obijeski, J. F., 481Ohishi, Iwao, 923Oliver, James D., 185Olpin, J., 100Oobayashi, Akira, 742Oroszlan, S., 100Ottaviano, Paul J., 271
Padhye, A. A., 218Pagano, J. F., 995Palmer, E. L., 481Palmer, Erskine L., 713Parisi, A., 41Parisi, Joseph T., 503Pariza, Michael W., 510Parkinson, A. J., 133Parkman, Paul D., 968Pazin, George J., 651Peleg, Ben-Ami, 1055Peters, Robert L., 614Petersen, Norman J., 822Pettiss, Katherine F., 156Plowman, J. K., 1040Pontefract, R. D., 717Poole, G. M., 154Porschen, Richard K., 851Puzniak, Constance I., 727
Queener, S. W., 498
Rabstein, Louise S., 614Ranhand, Jon M., 66Ray, C. George, 47,623Reece, Paul, 999Reinbold, G. W., 1076Rennie, R. P., 534Restrepo, Angela, 138Reynolds, Marcel C., 406Rhodes, R. A., 448, 845Richmond, Shirley, 912
Riley, P. S., 355, 1071Robbins, Ruth, 946Robertiello, Andrea, 737Roberts, Glenn D., 226Roberts, T. Richard, 91Robertson, E. Arthur, 691Romero, Redolfo, 199Ron, Nily, 1055Rosner, Richard, 245, 644Rowen, Joyce W., 720Rusmin, Simon, 347Russell, A. D., 331Russell, Philip K., 600
Sacks, Henry S., 374St. Julian, Grant 129Sakaguchi, Genji, 923Salanitro, J. P., 439Saldick, Jerome, 1004Salsbury, R. L., 106Sandine, W. E., 753Sands, D. C., 523Schaefer, J. 0., 151Schaeffer, David J., 605Schaffer, F. L., 280Scherer, R. K., 768Schipper, I. A., 1030Schleifer, Karl H., 867Schmidt, Nathalie J., 392, 400,
661Schoutens, E., 525Schreckenberger, Paul C., 759Schreiber, Minnie, 618Schroth, Milton N., 987Schwarz, J. R., 977, 982Schwert, David P., 1082Scott, Peter M., 892Seawright, Gary L., 802Self, Donnie J., 156Sell, Kenneth W., 326Shaparis, A. B., 262Sharpe, A. N., 223Shibatani, Takeji, 117Shulman, Jonas A., 778Silverman, Gerald J., 628Silverman, Melvin P., 507, 960Simpson, S., 608Smiley, J. J., 708Smith, Rodney F., 112, 359Sobsey, Mark D., 232, 861Soergel, M. E., 280Sommer, Noel F., 589Sompolinsky, David, 912Songer, J. Glenn, 359S0rensen, K. J., 553Souza, K. A., 1066Spahn, Gerard J., 614Spaulding, Earle H., 851Speck, M. L., 793, 1090Speirs, J. I., 717Spindler, Joyce A., 672Spira, Gad, 239Sroka, Jean, 1073Staneck, Joseph L., 226
Stanton, G. John, 145Steiner, Michael, 872Steinkraus, Keith H., 383Stevens, Roy, 1073Stevens, R. W., 306Stevenson, Walter R., 362Stewart, James E., 547, 915Still, P. E., 733Stoenner, Herbert G., 540Stuckey, H. M., 811Sudo, Sara Z., 582Sugimoto, Toshiyuki, 336Sullivan, James D., Jr., 1023Switzer, R. E., 1086
Talbot, Henry W., Jr., 503Tanabe, Osamu, 742Teh, J. S., 840Tepper, Byron S., 452Thatcher, Robert C., 887Thayer, D. W., 700Thomas, Frederick C., 802Thomas, S., 331Toma, S., 469Tompkin, R. B., 262Traci, P. A., 815Trieff, N. M., 359Trowbridge, R. S., 366Trust, T. J., 35, 340Tschider, S. R., 1030Tsuchiya, H. M., 831Tuite, John, 362Turnbill, C. E., 1066Tyler, J. E., 181Tzianabos, T., 481
Valenta, J. R., 995van de Voorde, H., 159Van Dijck, P., 159Varel, Vincent H., 251, 594Varela-Dfaz, Vfctor M., 1Vassall, John H., II, 623Vastine, David W., 688Vela, G. R., 77Viglia, Aurelio, 737Vitanza-Jack, Vilma B., 987Voll, M. J., 579
Wagner, Henry N., Jr., 452Walker, J. D., 982Wallis, Craig, 232, 861Wannamaker, Lewis W., 836Washington, John A., II, 303Wasilauskas, Benedict L., 91Watson, Stanley W., 1023Weaver, R. E., 355, 1071Weaver, Terry L., 887Webster, Janice M., 17Weiner, B. A., 448, 845Weiner, R. M., 579Welkos, Susan, 618Wende, Reuben D., 431White, James P., 1082
* *
VOL. 28, 1974
AUTHOR INDEX APPL. MICROBIOL.
Wiebe, W. J., 1033Wildfeuer, Alexander, 867Wilkinson, Hazel W., 199Willhight, Milton, 600Williams, Robert P., 431Windsor, G. D., 530Wolff, Katherine L., 323Wreghitt, T. G., 530
Wright, D. N., 205Wu, M. T., 1094
Yao, Raymond C., 52Yee, Robert B., 651Yen, T. F., 460Yetka, J. E., 1033Yokoyama, Sadaji, 742
Yoneda, Chieko, 688Young, Frank E., 70Young, Harold W., 929Young, Nancy P., 26Yourassowsky, E., 525
Zeikus, J. G., 258Ziegler, Donald W., 935
iv
SUBJECT INDEX
VOLUME 28
Acetamide agarnonfermentative bacteria, differentiation, 720
Acetylene reductionby Klebsiella isolated from effluents, 608by pulp and paper mill effluents, 608
Achlya heterosexualishormone B production, 544
Achromobacter from fishery sourcesphenethyl alcohol production, 681
Acid carboxypeptidaseP. janthinellum IFO-8070, 742
Acinetobacter calcoaceticus, compositioneffect of growth rate and nutrient limitation, 58
Acinetobacter, non-saccharolytic strainsnitrate reduction, 1071
Acriflavine treatmentS. lactis Lac- mutants, 753
Adenoviruses, serotypingimmune electron microscopy, 623
Adhesive tapenosocomial bacteria, 651
Aerobic actinomycetes, identificationthin-layer chromatography, 226
Aflatoxin biosynthesis, pathwaydichlorvos inhibition, 52
Alpha-glucosidase, formationPseudomonas SB15, 336
Alpha-toxins, purifiedS. aureus, 86
Anaerobic bacteriabiochemical testing, rapid method, 759
Anaerobic cocci, gram-positivefluorescent antibody study, 851
Antibioticsrespiratory inhibitors of bacterial populations,
1033Antimicrobial agents, zonal concentration gradi-
entbacterial colonies, 525
API Enteric 20 Profile Registeranalysis using a computer diagnostic model, 691
Arboviruses, California groupmicroculture plaque neutralization test, 802
Arthrobacter globiformisphage in soil, 951
Ascitic fluidsmouse interferon, 319
Aspergilli and penicillia, toxigenicaged, cured meats, 1094
Aspergillus flavusinfection in corn, 763
Aspergillus nigermalformin in onion bulbs, 362
Atmospheremonitoring in an anaerobic chamber, 582
Automated microtransferpoliovirus and mumps virus neutralizing anti-
bodies, assay, 968
Automated reagin testcolor-coded antigen, 317
Autotrophic bacteriairon oxidation, determination, 872
Azotobactersurvival in dry soil, 77
Bacillus, marineMnO2-reductase activity, 785
Bacillus pumilus, vegetativeradiation resistance, 41
Bacillus stearothermophilusin Icelandic soils, 638
Bacillus thuringiensiscarbohydrate metabolism, radiorespirometricsurvey, 129
minimal nutritional requirements, 124BACTEC systembacteremia detection, 435
Bacteremia detectionBACTEC system, 435
Bacteria, disruptionsimple, inexpensive procedure, 66
Bacterial coloniescount and size evaluation, 525
Bacterial extracellular polymer, determinationcritical-point drying, 312
Bacterial growtheffect of dispersants on crude oil, 547
Bacterial identificationquantitative infrared photoanalysis, 205
Bacterial respirationantibiotics as inhibitors, 1033
Bacterial spores, soilthermoradiation inactivation, 406
Bacterial toxins, microassaymultiple automated sample harvester, 326
Bacteriocin typing schemaC. perfringens, 172
Bacteriophage isolationfrom lysogenic bacteria, 503improved rapid plate method, 503
Bacteriuria, miniature testuse of dehydrated media and nitrite pads, 95
Bacteroides fragilisgrowth at controlled pH, 856
Bacteroides fragilis subsp. fragilisnutritional features, 251
Bacteroides melaninogenicus serogroupsdetermination by FA staining, 561
Bacteroides species, rumensphingolipid content, 1088
BBL Minitek systemEnterobacteriaceae, identification, 798
Beta-hemolytic streptococciextraction procedure for serological grouping,
836
v
SUBJECT INDEX
grouping directly on sheep blood agar plates,new method, 972
Biochemical testsanerobic bacteria, 759
Biophysical-immunological assayRNA type C viruses, 100
Blood culturesmacroscopic, microscopic, and radiometricexams, 641
Blood culture systems, evaluationuse of parallel culture methods, 245
Borrelia hermsiibiology in Kelly medium, 540
Bovine fecesgroup D streptococci, enumeration, 1086
Bovine mycoplasma antigensdetection by counterimmunoelectrophoresis, 897
Bovine omasumFMDV growth, 1055
Brewer's yeastsinhibition by wheat purothionin, 165
Brucella canis antibodiesgel-diffusion and tube agglutination tests, 1
Bunker C oilbacterial degradation, 915
Burn wound infectioneffect of a synthetic dressing, 465
Burn wound specimensselective isolation of yeasts, 112
['4C]aflatoxintissue distribution and metabolism, 1027
California (La Crosse) encephalitisdiagnosis by precipitin techniques, 807
Campylobacter fetus (Vibrio fetus)tube culture method for viable counts, 730
Carbohydrate metabolismB. thuringiensis, 129
Carotenoid pigmentsM. kansasii, 696
Cl compoundsPseudomonas C, growth, 906
Cecal microflora, commercial broiler chickens, 439Cerebrospinal fluid
gas-liquid chromatography, 298Chesapeake Bay microfloracomputer program to follow fluctuations, 185
Chlamydia trachomatisgrowth in Cyt B-treated McCoy cells, 912micro cell culture method, 727
Cholera vibrio biotyperapid method for determination, 586
Citrobacter, H2S-negativebiochemical and antibiotic susceptibility
studies, 22Cladosporium resinaeshort-chain fatty acids and alcohols, toxicity,
8408-Classtype tallying device and counter, 423Clostridium botulinum
in crabmeat from the blue crab, 722Clostridium botulinum type E toxinmolecular weight, 374
Clostridium botulinum progenitor toxinpurification, 923
Clostridium pasteurianumgrowth as function of sulfate concentration, 999
Clostridium perfringensbacteriocin susceptibility, 172cold shock lethality and injury, 815in the environment, 655
Clostridium perfringens enterotoxinligated intestinal loops in chickens, 889
Clostridium perfringens spores, heatedrecovery on selected media, 793
Coal refuse materialT. acidophilus ecology, 11
Coccidioidomycosis, diagnosisCF and ID tests, concomitant use, 641
Coenzyme AS. lutea, 117
Cold shock lethalityC. perfringens, 815
Coliform organismsrecovery on Gelman and Millipore filters, analy-
sis, 605radiometric method for detection, 169
Common urease testsfailure in isolation of P. productus, 594
Complement fixation and immunodiffusion testscoccidioidomycosis, diagnosis, 641
Complement-fixing antigenR. rickettsii, 481
Computer diagnostic modelAPI Enteric 20 Profile Register, analysis, 691
Continuous culturemedia optimization, 901
Corynebacterial kidney diseasesalmonids, 811
Corynebacteriumn-decane oxidation products, 737
Critical-point dryingbacterial extracellular polymer, determination,312
Crude oilbiodegradation and growth of bacteria, 547
Cyanuric acid biodegradation, 1004Cytochalasin BMcCoy cells, 912
Deep-sea bacteriahydrocarbons, growth and utilization, 982
Degradation, bacterialBunker C and crude oils and hydrocarbons, 915
Dehydrated mediaminiature test for bacteriuria, 95
Desulfovibrio speciessulfate reduction, 489
Dethiomethylation of methioninevitamin B6 involvement, 106
Diauxic growthP. shermanii, 831
2,4-Dichlorophenoxyacetic acidpseudomonad, growth rate, 181
Dichlorvos inhibitionaflatoxin biosynthesis, pathway, 52
Diketopiperazine formationP. italicum, 892
Dimethylselenideevolution from soils, 248
vi APPL. MICROBIOL.
SUBJECT INDEX
Disinfectionholding-tank sewage, 861
Dissolved oxygen conditions, controlledenterotoxin B and nuclease produwtion, 628
Dressing, syntheticcontrol of wound infection, 465
Drought, prairiemicrobial response, 700
Ehrlich ascites tumor preservationsimple method, 726
Electrical conductivity of soil solutionsextraterrestrial life, detection, 960
Electrophoresisdifferentiation of staphylococcal and micro-
coccal proteinases, 768Endotoxin detectionLimulus test, 867
Enteric virusesconcentration from water with lettuce extract,
717Enterobacteriaceae, identificationBBL Minitek system, evaluation, 668, 798
Enterococci in cheeseisolation and differentiation, 417
EnterotoxicityS. aureus strains, 946
Enterotoxin B and nuclease productioncontrolled dissolved oxygen conditions, 628
Enteroviruses in lake water, 895Epoxy-fiberglass absorbent
virus concentration, 501Epstein-Barr virusfrom P3HR-1 cells, 518
Escherichia coliH2S-producing variants, 303recovery from chlorinated secondary sewage,328
Escherichia coli antibodylatex test, 1073
Escherichia coli antigenslatex agglutination test, 306
Eutrophicationsewage treatment, 359
Extraterrestrial life, detectionchanges in electrical conductivity of soil solu-
tions, 960
Fatty acids, bacterialmethods for analysis, comparison, 80
Feedlot wasteindigenous organisms, growth, 448
Feedlot waste filtratefermentation by fungi and streptomycetes, 845
Filterscoliform organisms, recovery, 605
Fish waterpotential pathogens, occurrence, 35
Flat gel polyacrylamide electrophoresisporcine mycoplasmas, 530
Fluorescence microscopyhigh-quality microphotographs, 1063
Fluorescent antibody stainingB. melaniinogenicus serogroups, 561
Fluorescent antibody study
gram-positive anaerobic cocci, 851Food and feed enrichment
lactobacilli, lysine-excreting mutants, 523Foot-and-mouth disease virusgrowth in bovine omasum, 1055
Fungifeedlot waste filtrate, fermentation, 845
Fungi and yeastswater-stored cultures, 218
Gaseous end products, anaerobic metabolismradioisotopic analysis, 258
Gas-liquid chromatographyanalysis of sugars in cerebrospinal fluid, 298fatty acid analysis, 80
Gel-diffusion and tube agglutination testsB. canis antibody detection, 1
Glutaraldehydesporicidal activity, 331
Gnotobiotic salmonid fish, hatching, 340Gonococcal Genetic MediumN. gonorrhoeae, growth, 70
Gonorrhea, low incidenceGonosticon Dri Dot test, 431
Gonosticon Dri Dot test, evaluationgonorrhea, low incidence, 431
Group B streptococci, identificationimmunofluorescence staining, 199
Group D streptococcienumeration in bovine feces, 1086
Growth ratecomposition and biomass yield of A. calcoaceti-
cus, effect, 58
Haemophilus speciesmedia for isolation, 688
Hemadsorption inhibitionmicroadaptation, 547
Hemingrowth requirement for Naegleria, 64
Hemodialysis machinesmicrobial contamination of fluids, 822
Hepatitis B antigenradioimmunoassay, specificity, 600
Herpes simplex virus strains, typingindirect immunoperoxidase method, 568
Herpesvirus hominisIHA tests, 392solid phase radioimmunoassay, 661
Herpesvirus hominis, antibodies and isolatestyping by IHA inhibition, 400
Heterotrophic and enteric bacteriasorption to glass surfaces, 572
High-molecular-weight alkanesmicrobial degradation, 1084
Homocitrate synthaseP. chrysogenum extracts, 265
Homogeneous spore suspensionsmethod for preparation, 498
Hormone B productionA. heterosexualis, 544
Hydrocarbonsgrowth at ambient and in situ pressure, 982
Hydrogen sulfide-producing coloniesTergitol-7 agar for differentiation, 521
*i-VOL. 28, 1974
SUBJECT INDEX
Hydrogen-sulfide producing variantsE. coli, 303
Hydrophobic grid-membrane filtersimproved microbiological enumeration, 223
Hydrostatic pressureeffect on V. parahaemolyticus, 977
12I- and [3H]acetate-labeled antibodiesiridescent virus detection, 133
Ice nucleationP. syringae, 456
Immune electron microscopyadenoviruses, serotyping, 623
Immunodiffusion test for paracoccidioidomycosischaracterization of precipitin bands, 138
Immunofluorescence staininggroup B streptococci, identification, 199
Immunofluorescent cell-counting assaylymphocytic choriomeningitis virus, 17
Immunoperoxidasemyxoviruses and paramyxoviruses, identifica-
tion, 47InactivationMVM stability, 351
Indigenous organisms, growthfeedlot waste, 448
Indirect fluorescent-antibody techniqueNaegleria sp., 159
Indirect hemagglutination inhibitionH. hominis, typing, 400
Indirect hemagglutination testsH. hominis types 1 and 2, 392
Indirect immunoperoxidase methodherpes simplex virus strains, typing, 568
Infectious bovine rhinotracheitis virusmembrane concentration, 1030
Infrared photoanalysisbacterial identification, 205
Interferon, mouseascitic fluids, 319
Intravenous infusion fluidin-use contamination, 778
In-use contaminationi.v. infusion fluid, 778
In vitro metabolismradiometric measurement, 177
lodinated globulinspreparation for RIA, 935
Iridescent virus detection1251- and [3H]acetate-labeled antibodies, 133
Iron oxidationdetermination by autotrophic bacteria, 872
Isoamylase, formationPseudomonas SB15, 336
Isolation of yeasts, selectiveevaluation of media, 112
Kelly mediumB. hermsii, biology, 540
Klebsiellaacetylene reduction, 608biochemical and serological typing, 534natural receiving waters, 672
Lactobacilli, lysine-excreting mutantsuse in food and feed enrichment, 523
Lactobacillus bulgaricusrecovery on agar media, 1076
Lac+ transductantsS. lactis, 753
Laminar flow biological safety cabinet, modified,647
Latex agglutination testE. coli antigens, detection, 306
Latex testE. coli antibody, determination, 1073
Leptospira biflexabacteriological media, contamination, 505
Limulus lysatefactors affecting sensitivity, 1023
Limulus testendotoxin detection, 867
Liquid scintillation radioassaydisposable microcentrifuge tubes, 280
Liquid scintillation vialin vitro metabolism, measurement, 177
Lymphocytic choriomeningitis virusimmunofluorescent cell-counting assay, 17
McCoy cellsC. trachomatis, growth, 912
Maidi-progressive pneumonia-visna virusesplaque assay, 366
MalforminA. niger-infected onion bulbs, 362
Malting and brewing experimentsfate of ochratoxin A and citrinin, 31
Manganese dioxide-reductase activityseawater cations and temperature, effects, 785
Manganous chloriderubella hemagglutination inhibition, 992
Media optimizationuse of continuous culture, 901
Membrane filtersautoclave and ethylene oxide-sterilized, com-
parison, 474Metabolic activityM. lepraemurium, 452
Methane-oxidizing bacteriagas metabolism and growth, concurrent meas-urement, 507
Microbial contamination of fluids, 822Microbial ecology, fluctuationscomputer program, 185
Microbial transformationmethyl 5(6)-butyl-2-benzimidazolecarbamate,995
Microbiological enumeration, improvedhydrophobic grid-membrane filters, 223
Microcentrifuge tubes, disposableliquid scintillation radioassay, applications, 280
Microculture plaque neutralization testCalifornia-group arboviruses, 802
Microorganismsgrowth under extremely alkaline conditions, 1066
Microorganisms, critical-point dryingspecimen holder for SEM, 708
* * @
APPL. MICROBIOL.
SUBJECT INDEX
Milk culturesS. aureus and S. typhimurium, 1090
Minitek systemEnterobacteriaceae, identification, 668
Minute virus, micestability to chemical and physical agents, 351
Modified spinning top spray apparatusrespiratory disease studies, 929
Molecular weightC. botulinum type E toxin, 374
Moraxella urethralis59 strains, characterization, 355
Mouse mammary tumor virusproduction, in vitro system, 1040
Multiple automated sample harvesterbacterial toxins, microassay, 326
Mumps virus neutralizing antibodiesautomated microtransfer, 968
Murine leukemia viruses, detectionin vivo assay, 614
Murine sarcoma-leukemia virus group-specificantigen
radioimmunoassay, 239Mycobacterium aviumresponse to UV irradiation, 151
Mycobacterium chelonei subspecies abscessusisolation from pus, 943
Mycobacterium kansasiicarotenoid pigments, 696
Mycobacterium lepraemuriummetabolic activity, 452
Mycobacterium paratuberculosisgrowth and metabolic characteristics, 276
Myxoviruses and paramyxovirusesidentification by using immunoperoxidase, 47
Naegleriahemin as growth requirement, 64
Naegleria sp., identificationindirect fluorescent-antibody technique, 159
Nalidixic acid enrichmentS. typhimurium, 579
Natural receiving watersKlebsiella isolates, 672
n-Decane oxidation productsCorynebacterium cultures, 737
Neisseria gonorrhoeae, growthdefined minimal medium, 70
Neisseria speciesTMPD oxidase reaction, 1079
Newcastle disease virus infectionviral envelope, influence, 26
Nitrate reductionAcinetobacter, 1071
Nitrification, in situ measurement, 1082Nocardia caviaenew isolations, 193
Nonfermentative bacteriaacetamide agar for differentiation, 720
n-immunological precipitation of serumy SDS in agar diffusion, 557
Nosocomial bacteriaadhesive tape, 651
Nutrional requirementsB. fragilis subsp. fragilis, 251
0-1 bacteriophageSalmonella identification, 618
Ochratoxin A and citrininfate during malting and brewing experiments, 31
Oil shale, degradationsulfur-oxidizing bacteria, 460
Paper-strip blood-sampling techniqueantibody to Y. pestis, detection, 323
Parabiotic chamber for organ cultures, improved,774
Parathiondecontamination in aqueous media, 212
Parbendazolemicrobial transformation, 995
Pathogens, potentialoccurrence in fish water, 35
Patulin productionP. expansum, 589
Penicillium chrysogenumhomocitrate synthase, insensitivity, 265
Penicillium expansumpatulin production, 589
Penicillium italicumdiketopiperazine formation, 892
Penicillium janthinellumacid carboxypeptidase, submerged production,742
Penicillium stoloniferum virusconcentration by polyethylene glycol, 733
Peptostreptococcus productus, ureolyticisolation from feces using defined medium, 594
Periplasmic bodiesS. rouxii (Boutroux), 1047
pH, controlledB. fragilis growth, 856
Phenethyl alcohol productionAchromobacter from fishery sources, 681
P3HR-1 cellsEpstein-Barr virus, 518
Pig hemagglutinating encephalomyelitis virushemadsorption inhibition, microadaptation, 553
Plant and soil reservoirsP. aeruginosa, 987
Plaque assaymaidi-progressive pneumonia-visna viruses, 366
p-Nitrophenoldecontamination in aqueous media, 212
Poliovirus neutralizing antibodiesautomated microtransfer, 968
Polyethylene glycolP. stoloniferum virus, concentration, 733
Porcine mycoplasmasflat gel polyacrylamide electrophoresis, 530
Potassium sorbateeffect on salmonellae, 262
Precipitin bands, characterizationimmunodiffusion test for paracoccidioidomy-
cosis, 138
VOL. 28, 1974 1X
SUBJECT INDEX
Precipitin techniquesCalifornia (La Crosse) encephalitis, diagnosis,807
Propionibacterium shermaniidiauxic growth, 831
Propylene oxidesterilizing agent, 1069
Proteinase- and lactose-utilizing enzymesS. lactis, 342
Proteinases, staphylococcal and micrococcaldifferentiation by electrophoresis, 768
Pseudomonadgrowth on 2,4-dichlorophenoxyacetic acid and
2,4-dichlorophenol, 181Pseudomonas aeruginosaplant and soil reservoirs, 987
Pseudomonas Cgrowth on C1 compounds, 906
Pseudomonas SB15isoamylase and a-glucosidase, formation, 336
Pseudomonas syringaeice nucleation, 456
Radiation resistancevegative B. pumilus, 41
Radioimmunoassayhepatitis B antigen, 600iodinated globulins, 935murine sarcoma-leukemia virus gs antigen, 239
Radioisotopic analysisgaseous end products of anaerobic metabolism,
258Respiratory disease studies
use of modified spinning top homogeneous sprayapparatus, 929
Rhizopus oligosporusinoculum for tempeh fermentation, 347
Ribonucleic acid type C virusesbiophysical-immunological assay, 100
Rickettsia akariultrastructure, 713
Rickettsia-like organisminfection of freshwater amphipod, 585
Rickettsia prowazekiultrastructure, 713
Rickettsia rickettsiicomplement-fixing antigen, 481
River water, continuous cultureheterotropic and enteric bacteria, sorption, 572
Rubella hemagglutination inhibitionnonspecific agglutination removal, 992
Ruminococcus bromiinutritional features, 1018
Saccharomyces cerevisiaeeffect of dissolved oxygen, cell count, and sugar
concentration, 383viability in rapid fermentations, 383
Saccharomyces rouxiiperiplasmic structure, 1047
Salmonellaidentification with 0-1 phage, 618
Salmonellaepotassium sorbate, effect, 262
Salmonella enteritidisisolation from eutrophic regions of a freshwater
lake, 723Salmonella 0 and Shigella antisera, preparation
reuse of Salmonella and Shigella absorbing cells,320
Salmonella typhimuriumnalidixic acid enrichment, 579
Salmonid roe, sterility, 340Salmonids
corynebacterial kidney disease, causative bac-terium, 811
Sampling technique, large volume, 515Sarcina luteacoenzyme A, production, 117
Scanning electron microscopymicroorganisms, critical-point drying, 708
Seasonal conjunctivitis casesHaemophilus isolation, 688
Selective enterococcus mediummanganese ion deficiency, sodium azide, and al-
kaline pH, 411Serological groupingbeta-hemolytic streptococci, 836
SerotypingKlebsiella isolates, 534
Serratia speciesdistribution in clinical specimens, 513
Sewage, holding tankchemical disinfection, 861
Sewage treatmentcontrolled eutrophication, 359
Sheep blood agar platesgrouping beta-hemolytic streptococci, 972
5% Sheep blood agar plates, preparationsodium polyanethol sulfonate, 91
Silica gel media, preparationsimplified method, 887
Sodium dodecyl sulfatenon-immunological precipitation of serum, 557
Sodium polyanethol sulfonate5% sheep blood agar plates, preparation, 91
SoilA. globiformis phage, 951
Solid phase radioimmunoassayH. hominis, identification, 661
Solid waste landfill leachatesviruses, detection, 232
Sphingolipid contentrumen Bacteroides sp., 1088
Sporeforming bacteria, physiology, 124, 129Sporicidal activity
glutaraldehyde, 331Staphylococcus aureus
enterotoxicity detection, 946purified alpha-toxins, comparison, 86selected typing bacteriophages, genome size, 510staphylokinase, complex form, 5
Staphylokinase, complex formoccurrence in S. aureus, 5
Streptococci, lacticantagonism toward S. aureus and S. typhi-murium in milk cultures, 1090
Streptococcus lactis
x APPL. MICROBIOL.
SUBJECT INDEX
lac prt- mutants, 342LAC+ transductants, 753
Streptococcus thermophilusrecovery on agar media, 1076
Sulfate concentrationfunction of C. pasteurianum growth, 999
Sulfate reductionDesulfovibrio species from sheep rumen, 489
Sulfur-oxidizing bacteriaoil shale, degradation, 460
Surface disinfectantsvirucidal efficacy, 748
Susceptibility studies, biochemical and antibioticH2S-negative Citrobacter, 22
Syphilisautomated reagin test, 317
Tempeh fermentationrice-grown R. oligosporus, 347
Tetramethyl-p-phenylenediamine oxidase reactionNeisseria sp., 1079
Thermoplasma acidophilumecology in coal, 11
Thermoradiation inactivationspores in soil, 406
Thin-layer chromatographyaerobic actinomycetes, 226
Tissue distribution[14C]aflatoxin in chickens, 1027
Trace element levelsA. flavus infection and aflatoxin production in
corn, 763Trichophytin extraction
T. mentagrophytes grown in a complex and a de-fined medium, comparison, 271
Trichophyton mentagrophytestrychophytin extraction, 271
Trypsin solutions, sterile filtered and heat-steri-lized
factors influencing activity, 154
Venezuelan equine encephalomyelitis virusvirulence for laboratory hosts, 881
Vibrio parahaemolyticusdistribution in Atlantic Ocean, 1009hydrostatic pressure, effect, 977
Viral envelopeNewcastle disease virus infection, 26
Viral infectivity assaysstaining and eliminating focus formation, im-proved, 156
Virucidal efficacythree common liquid surface disinfectants, 748
Virus detectionsolid waste landfill leachates, 232
Vitamin B6dethiomethylation of methionine, 106
Volatile compounds, high boilingproduced during storage of haddock fillets, 679
Water qualitySpoon River, bacteriological assessment, 288
Water storagefungi, yeasts, and aerobic actinomycetes, 218
Western equine encephalitis virus plaque variantsdetection in crowded cultures and plaqueprogeny, 145
Wheat purothioninsinhibition of brewer's yeasts, 165
Yersinia enterocolitica infectionisolation, in Canada, 469
Yersinia pestis antibody, detectionpaper-strip blood-sampling technique, 323
VOL. 28, 1974 Xi
INDEX TO DATE OF ISSUE
Month Date of Issue Pages
July 31 July 1974 1-157August 27 August 1974 159-330September 30 September 1974 331-524October 25 October 1974 525-735November 23 November 1974 737-899December 3 January 1975 901-1096
APPLIED
MICROBIOLOGY
VOLUME 28
BALTIMORE, MD. 21202
1974
APPLIED MICROBIOLOGYVOLUME 28 * 1974
EDITORIAL BOARD
MARVIN P. BRYANT, Editor-in-Chief (1976)University of Illinois, Urbana
ALBERT BALOWS, Editor (1975)Center for Disease Control,
Atlanta, Ga.
R. H. DEIBEL, Editor (1976)University of Wisconsin, Madison
A. L. DEMAIN, Editor (1976)Massachusetts Institute of Technology, Cambridge
NATHALIE J. SCHMIDT, Editor (1975)California State Department of
Public Health, Berkeley
JAMES M. TEEDJE, Editor (1979)Michigan State University,
East Lansing
Bernard J. Abbott (1977)Libero Ajello (1976)Milton J. Allison (1974)Richard Bartha (1976)Martha D. Berliner (1977)Jerome Birnbaum (1976)Lee A. Bulla, Jr. (1977)Francis F. Busta (1975)William R. Chesbro (1976)Alex Clegler (1974)Rita R. Colwell (1974)Richard A. Consigli (1976)Charles Cooney (1976)Hugo L. David (1975)Jacob A. Donkersloot (1976)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1975)Richard Elander (1976)Bernice England (1977)Martin S. Favero (1975)John C. Feeley (1974)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Morris Fishbein (1976)Heinz G. Floss (1977)Edwin E. Geldreich (1976)S. E. Gilliland (1974)John M. Goepfert (1976)
Charles T. Goodhue (1977)Morris A. Gordon (1975)Robert D. Hamilton (1976)Richard S. Hanson (1975)James L. Hardy (1975)Paul A. Hartman (1974)Melvin T. Hatch (1975)Clifford W. Hesseltine (1974)Lillian V. Holdeman (1976)John J. Iandolo (1974)M. B. Ingle (1976)Henry D. Isenberg (1974)Holger W. Jannasch (1975)Donald E. Jensen (1977)S. S. Kalter (1975)Edward Katz (1976)Lloyd L. Kempe (1976)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Barbara D. Lago (1977)Hubert Lechevalier (1976)Paul Lemke (1976)John Liston (1974)Warren Litsky (1975)Robert Mah (1974)William J. Martin (1975)Joseph L. Melnick (1976)William I. Metzger (1974)Thomas L. Miller (1976)
David Paretsky (1976)W. J. Payne (1976)D. Perlman (1974)David Pramer (1974)Antonio H. Romano (1977)E. L. Schmidt (1974)Oldrich K. Sebek (1974)Warren S. Silver (1976)Anthony J. Sinskey (1976)Louis DS. Smith (1975)Peter B. Smith (1976)S. S. Socransky (1975)Alex C. Sonnenwirth (1975)Mortimer P. Starr (1975)Guenther Stotzky (1974)Robert B. Tompkin (1976)Marvin Turck (1974)Carl Vanderzant (1976)Claude Vezina (1976)D. I. C. Wang (1975)Lewis W. Wannamaker (1975)John A. Washington H (1975)Robert E. Weaver (1975)Sydney J. Webb (1975)Fred D. Williams (1977)R. P. Williams (1975)Benjamin J. Wilson (1975)G. N. Wogan (1974)Alan G. Wolin (1974)
ROBERT A. DAY, Managing EditorGISELLA POLLACK, Director of Editorial Services
CHERYL A. CROSS, Production Editor1913 1 St., N.W., Washington D.C. 20006
EX OFFICIOPHILIPP GERHARDT, President (1974-1975) HELEN R. WHITELEY, Vice-President (1974-1975)
J. MEHSEN JOSEPH, SecretaryApplied Microbiology, a publication of the American Society
for Microbiology, 1913 I St., N.W., Washington, D.C. 20006, isdevoted to the advancement and dissemination of applied knowl-edge as well as ecological knowledge, both applied and funda-mental, concerning microorganisms. Applied Microbiology is pub-lished monthly, two volumes per year. The subscription price is$60 (foreign, $61) per year. Single copies are $6.00 (foreign,$6.25). Members of the American Society for Microbiology mayreceive Applied Microbiology as part of their dues. Correspond-ence relating to subscriptions, nonreceipt of journals, reprints, de-fective copies, availability of back issues, and lost or late proofsshould be directed to the ASM Publications Office, 1913 I St.,
T. J. CARSKI, TreasurerN.W., Washington, D.C. 20006 (area 202 833-9416). Correspond-ence from ASM members relating to membership dues, membersubscriptions, changes of address, incorrect address, incorrectjournals, etc., should be directed to the Executive Secretary,American Society for Microbiology, 1913 I St., N.W., Washington,D.C. 20006. Published monthly by the ASM at 1913 I St., N.W.,Washington, D.C. 20006.
Second-class postage paid at Washington, D.C. 20006, andat additional mailing offices. Made in the United States ofAmerica.Copyright @) 1975, American Society for Microbiology.All Rights Reserved.
Volume 28 Contents for July Number 1
Environmental Microbiology and EcologyImmunofluorescence Approach to the Study of the Ecology of Thermoplasma acido-
philum in Coal Refuse Material. B. B. BOHLOOL AND T. D. BROCK.......... 11Occurrence of Potential Pathogens in Water Containing Ornamental Fishes. T. J.
TRUST AND K. H. BARTLETT.............................................. 35Survival of Azotobacter in Dry Soil. G. R. VELA............................... 77
Food Microbiology and ToxicologyFate of Ochratoxin A and Citrinin During Malting and Brewing Experiments. P.
KROGH, B. HALD, P. GJERTSEN, AND F. MYKEN............................. 31
Metabolism and ProductsOccurrence of a Complex Form of Staphylokinase in the Course of Cultivation of
Staphylococcus aureus. SETSUO FUJIMURA, TOSHIKAZU MAKINO, AND TAKAYOSHIT. A. HAYASHI............................................ ............ 5
Increased Radiation Resistance of Vegetative Bacillus pumilus. A. PARISI AND A. D.ANTOINE ............................................................... 41
Step of Dichlorvos Inhibition in the Pathway of Aflatoxin Biosynthesis. RAYMONDC. YAO AND DENNIS P. H. HSIEH .......................... ........... 52
Effect of Growth Rate and Nutrient Limitation on the Composition and BiomassYield of Acinetobacter calcoaceticus. BERNARD J. ABBOTT, ALLEN I. LASKIN, ANDC. J. McCoy .......................................................... 58
Hemin Replaces Serum as a Growth Requirement for Naegleria. R. NEAL BAND ANDWILLIAM BALAMUTH............................. ........................64
Simple, Inexpensive Procedure for the Disruption of Bacteria. JON M. RANHAND ... 66Comparison of Rapid Methods for Analysis of Bacterial Fatty Acids. C. WAYNE
Moss, M. A. LAMBERT, AND W. H. MERWIN............................... 80Involvement of Vitamin B6 in the Dethiomethylation of Methionine by Rumen Micro-
organisms. D. L. MERRICKS AND R. L. SALSBURY......................... 106Production of Coenzyme A by Sarcina lutea. NORIYUKI NISHIMURA, TAKEJI SHI-
BATANI, TOSHIO KAKIMOTO, AND ICHIRO CHIBATA........................... 117Physiology of Sporeforming Bacteria Associated with Insects: Minimal Nutritional
Requirements for Growth, Sporulation, and Parasporal Crystal Formation ofBacillus thuringiensis. KENNETH W. NICKERSON AND LEE A. BULLA, JR....... 124
Physiology of Sporeforming Bacteria Associated with Insects: RadiorespirometricSurvey of Carbohydrate Metabolism in the 12 Serotypes of Bacillus thuringiensis.KENNETH W. NICKERSON, GRANT ST. JULIAN, AND LEE A. BULLA, JR.......... 129
Clinical Microbiology and ImmunologyComparative Sensitivity of Gel-Diffusion and Tube Agglutination Tests for the De-
tection of Brucella canis Antibodies in Experimentally Infected Dogs. DONALDM. MYERS, VICTOR M. VARELA-DIAZ, AND EMILIo A. COLTORTI.1
Biochemical and Antibiotic Susceptibility Studies of H2S-Negative Citrobacter.MARLYS E. LUND, JOHN M. MATSEN, AND DONNA J. BLAZEVIC................ 22
Development of a Defined Minimal Medium for the Growth of Neisseria gonorrhoeae.LEONARD J. LA SCOLEA, JR., AND FRANK E. YOUNG......................... 70
Comparison of Purified Alpha-Toxins from Various Strains of Staphylococcus aureus.RONALD L. GOODE AND JACK N. BALDWIN.................................. 86
Use of Sodium Polyanethol Sulfonate in the Preparation of 5% Sheep Blood AgarPlates. BENEDICT L. WASILAUSKAS, JULIA FLOYD, AND T. RICHARD ROBERTS. 91
Evaluation of Miniature Test for Bacteriuria Using Dehydrated Media and NitritePads. CRAIG M. MOFFAT, MICHAEL R. BRITT, AND JOHN P. BURKE........... 95
Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and BurnWound Specimens. RODNEY F. SMITH, DIANNA BLASI, AND SANDRA L. DAYTON. 112
CONTENTS xv
Characterization of the Precipitin Bands Detected in the Immunodiffusion Test forParacoccidioidomycosis. ANGELA RESTREPO AND Luz H. MONCADA.......... 138
Response of Mycobacterium avium to Ultraviolet Irradiation. C. M. MCCARTHY ANDJ. 0. SCHAEFER......................................................... 151
Virology and Viral ImmunologyImmunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus.
JANICE M. WEBSTER AND B. E. KIRK...................................... 17Influence of Viral Envelope on Newcastle Disease Virus Infection. NANCY P.
YOUNG AND RONALD J. ASH..................................... ..........26Use of Immunoperoxidase for the Rapid Identification of Human Myxoviruses and
Paramyxoviruses in Tissue Culture. DENIS R. BENJAMIN AND C. GEORGE RAY. . 47Biophysical-Immunological Assay for Ribonucleic Acid Type C Viruses. J. OLPIN,
S. OROSZLAN, AND R. V. GILDEN......................................... 100Comparative Study of [l25I]- and [3H]Acetate-Labeled Antibodies in Detecting Iri-
descent Viruses. S. L. BILIMORIA, A. J. PARKINSON, AND J. KALMAKOFF........ 133Enhanced Detection of Western Equine Encephalitis Virus Plaque Variants in
Crowded Cultures and Plaque Progeny-Potential Use in Genetic Studies. G.JOHN STANTON, DEBORAH CHARSHA MAY, AND S. C. DE ST. JEOR.............. 145
Factors Influencing the Activity of Sterile Filtered and Heat-Sterilized Trypsin-Solu-tions. R. A. COOK AND G. M. POOLE..................................... 154
Improved Techniques for Staining and Enumerating Focus Formation in Viral In-fectivity Assays. DONNIE J. SELF, KATHERINE F. PETTISS, AND DARELL D.BIGNER................................................................ 156
Volume28 Contents for August Number2
Environmental Microbiology and EcologyRadiometric Method for the Detection of Coliform Organisms in Water. URIEL
BACHRACH AND ZELILAH BACHRACH....................................... 169Computer Program Designed to Follow Fluctuations in Microbial Populations and
Its Application in a Study of Chesapeake Bay Microflora. JAMES D. OLIVERAND R. R. COLWELL..................................................... 185
Quantitative Infrared Photoanalysis of Selected Bacteria. D. L. DUGAN AND D. N.WRIGHT............................................ ................... 205
Microbial Decontamination of Parathion and p-Nitrophenol in Aqueous Media.DOUGLAS M. MUNNECKE AND DENNIS P. H. HSIEH......................... 212
Hydrophobic Grid-Membrane Filters: New Approach to Microbiological Enumera-tion. A. N. SHARPE AND G. L. MICHAUD.................................. 223
Development of Methods for Detecting Viruses in Solid Waste Landfill Leachates.MARK D. SOBSEY, CRAIG WALLIS, AND JOSEPH L. MELNICK.................. 232
Evolution of Dimethylselenide from Soils. A. J. FRANCIS, J. M. DUXBURY, ANDM. ALEXANDER......................................................... 248
Nutritional Features of Bacteroides fragilis subsp. fragilis. VINCENT H. VAREL ANDMARVIN P. BRYANT..................................................... 251
Rapid Method for the Radioisotopic Analysis of Gaseous End Products of AnaerobicMetabolism. DAVID R. NELSON AND J. G. ZEIKUS......................... 258
Bacteriological Assessment of Spoon River Water Quality. SHUNDAR LIN, RALPH L.EVANS, AND DAVIS B. BEUSCHER.......................................... 288
Recovery of Escherichia coli from Chlorinated Secondary Sewage. J. R. BRASWELLAND A. W. HOADLEY.................................................... 328
Food Microbiology and ToxicologyInhibition of Brewer's Yeasts by Wheat Purothionins. C. HERNANDEZ-LUCAS, R.
FERNANDEZ DE CALEYA, AND PILAR CARBONERO............................ 165
xvi CONTENTS
Effect of Potassium Sorbate on Salmonellae, Staphylococcus aureus, Clostridium per-fringens. and Clostridium botulinum in Cooked, Uncured Sausage. R. B. TOMP-KIN, L. N. CHRISTIANSEN, A. B. SHAPARIS, AND H. BOLIN.................... 262
Use of Microculture Plates and the Multiple Automated Sample Harvester for InVitro Microassay of Bacterial Toxins. RICHARD C. KNUDSEN, LYNN T. CALLA-HAN III, AFTAB AHMED, AND KENNETH W. SELL........................... 326
Metabolism and ProductsLiquid Scintillation Vial for Cumulative and Continuous Radiometric Measurement
of In Vitro Metabolism. EDWARD U. BUDDEMEYER......................... 177Growth Rates of a Pseudomonad on 2,4-Dichlorophenoxyacetic Acid and 2,4-Di-
chlorophenol. J. E. TYLER AND R. K. FINN. . . .. . ......................... 181Storage of Stock Cultures of Filamentous Fungi, Yeasts, and Some Aerobic Actino-
mycetes in Sterile Distilled Water. M. R. McGINNIs, A. A. PADHYE, AND L.AJELLO. ............................................................... 218
Insensitivity of Homocitrate Synthase in Extracts of Penicillium chrysogenum toFeedback Inhibition by Lysine. PRAKASH S. MASUREKAR AND ARNOLD L.DEMAIN.................................. ............................. 265
Growth and Metabolic Characteristics of Mycobacterium paratuberculosis. R. S.MERKAL AND B. J. CURRAN.............................................. 276
Critical-Point Drying: a Rapid Method for the Determination of Bacterial Extra-cellular Polymer and Surface Structures. GERALD D. CAGLE................ 312
Clinical Microbiology and ImmunologyEvaluation of the Indirect Fluorescent-Antibody Technique for Identification of
Naegleria Species. J. DE JONCKHEERE, P. VAN DIJCK, AND H. VAN DE VOORDE. 159Bacteriocin Susceptibility of Clostridium perfringens: a Provisional Typing Schema.
D.E.MAHONY.......................................................... 172Nocardia caviae: a Report of 13 New Isolations with Clinical Correlation. WILLIAM
A. CAUSEY............................................................. 193Identification of Group B Streptococci by Immunofluorescence Staining. REDOLFO
ROMERO AND HAZEL W. WILKINSON... 199Simplified Approach to Identification of Aerobic Actinomycetes by Thin-Layer
Chromatography. JOSEPH L. STANECK AND GLENN D. ROBERTS.............. 226Evaluation of Four Blood Culture Systems Using Parallel Culture Methods. RICHARD
ROSNER.............................................................. 245Trichophytin Extraction: Biological Comparison of Trichophytin Extracted from
Trichophyton mentagrophytes Grown in a Complex Medium and a Defined Me-dium. PAUL J. OTTAVIANO, HENRY E. JONES, JUNE JAEGER, ROBERT D. KING,AND DAVID BIBEL....................................................... 271
Rapid Diagnosis of Infection by Gas-Liquid Chromatography: Analysis of Sugars inNormal and Infected Cerebrospinal Fluid. STANLEY AMUNDSON, ABRAHAM I.BRAUDE, AND CHARLES E. DAVIS.......................................... 298
Hydrogen Sulfide-Producing Variants of Escherichia coli. MYRON D. MAKER ANDJOHN A. WASHINGTON II................................................. 303
Detection of Escherichia coli Antigens by a Latex Agglutination Test. K. HECHEMY,R. W. STEVENS, AND H. A. GAAFAR . ........................... 306
Color-Coded Antigen for the Automated Reagin Test for Syphilis. S. S. KASATIYAAND N. G. LAMBERT..................................................... 317
Reuse of Salmonella and Shigella Absorbing Cells for Preparing Monospecific Sal-monella 0 and Shigella Antisera. SAMUEL B. GRAY, JR., JAMES H. GREEN,WILLIAM K. HARRELL, LOIS BRITT, AND RICHARD C. BOLIN.................. 320
Paper-Strip Blood-Sampling Technique for the Detection of Antibody to the PlagueOrganism Yersinia pestis. KATHERINE L. WOLFF AND BRUCE W. HUDSON..... 323
Virology and Viral ImmunologyModified Radioimmunoassay for Murine Sarcoma-Leukemia Virus Group-Specific
*iiCONTENTS
Antigen. GAD SPiRA, NILAMBAR BISWAL, AND GORDON E. DREESMAN......... 239Liquid Scintillation Radioassay in Disposable Microcentrifuge Tubes: Radioimmune
Precipitates and Other Applications. F. L. SCHAFFER AND M. E. SOERGEL..... 280Mouse Interferon in Ascitic Fluids. SONJA M. BUCKLEY AND JORDI CASALS........ 319Erratum................................................................... 330
Volume28 Contents for September Number3
Environmental Microbiology and EcologySterility of Salmonid Roe and Practicality of Hatching Gnotobiotic Salmonid Fish.
T. J. TRUST............... J.340
Sewage Treatment by Controlled Eutrophication: Bacterial Study. J. GLENNSONGER, RODNEY F. SMITH, AND N. M. TRIEFF............................ 359
Thermoradiation Inactivation of Naturally Occurring Bacterial Spores in Soil.MARCEL C. REYNOLDS, KERMIT F. LINDELL, THOMAS J. DAVID, MARTIN S.FAVERO, AND WALTER W. BOND.......................................... 406
8-Classtype Tallying Device and Counter. JAMES N. ADAMS.................... 423Studies on the Cecal Microflora of Commercial Broiler Chickens. J. P. SALANITRO,
I. G. BLAKE, AND P. A. MUIRHEAD........................................ 439Growth of Indigenous Organisms in Aerated Filtrate of Feedlot Waste. B. A. WEINER
AND R. A. RHODES......................................................448Ice Nucleation Induced by Pseudomonas syrtngae. LEROY R. MAKI, ELIZABETH L.
GALYAN, MEI-MON CHANG-CHIEN, AND DANIEL R. CALDWELL.............. 456Comparison of Autoclave and Ethylene Oxide-Sterilized Membrane Filters Used in
Water Quality Studies. B. J. DUTKA, M. J. JACKSON, AND J. B. BELL........ 474Sulfate Reduction by a Desulfovibrio Species Isolated from Sheep Rumen. JOELLEN
HUISINGH, J. J. MCNEILL, AND G. MATRONE............................. 489Epoxy-Fiberglass Adsorbent for Concentrating Viruses from Large Volumes of Potable
Water. WALTER JAKUBOWSKI, JOHN C. HOFF, NATHANIEL C. ANTHONY, ANDWILLIAM F. HILL, JR.................................................... 501
Quantitative Large-Volume Sampling Technique. M. A. LEVINE, J. R. FISCHER,AND V. J. CABELLI...................................................... 515
Food Microbiology and ToxicologySimultaneous Loss of Proteinase- and Lactose-Utilizing Enyzme Activities in Strepto-
coccus lactis and Reversal of Loss by Transduction. L. L. MCKAY AND K. A.BALDWIN........................ ........ 342
Rice-Grown Rhizopus oliosporus Inoculum for Tempeh Fermentation. SIMONRUSMIN AND SWAN DJIEN Ko........................................... 347
Malformin in Aspergitlus niger-Infected Onion Bulbs (Allium cepa). ROY W. CURTIS,WALTER R. STEVENSON, AND JOHN TUITE .................................. 362
Clostridium botulinum Type E Toxin: Effect of pH and Method of Purification onMolecular Weight. HENRY S. SACKS AND SCOTT V. COVERT .................. 374
Effect of Dissolved Oxygen, Temperature, Initial Cell Count, and Sugar Concentrationon the Viability of Saccharomyces cerevisiae in Rapid Fermentations. TILAK W.NAGODAWITHANA, CARMINE CASTELLANO, AND KEITH H. STEINKRAUS .... 383
Development of a Selective Enterococcus Medium Based on Manganese Ion De-ficiency, Sodium Azide, and Alkaline pH. C. J. EFTHYMIOU AND S. W. JOSEPH.. 411
Improved Isolation and Differentiation of Enterococci in Cheese. C. J. EFTHYMIOU,P. BACCASH, V. J. LABOMBARDI, AND D. S. EPSTEIN......................... 417
Selecting Lysine-Excreting Mutants of Lactobacilli for Use in Food and Feed En-richment. D. C. SANDS AND LESTER HANKIN.............................. 523
* *i. CONTENTS
Metabolism and ProductsFormations of Extracellular Isoamylase and Intracellular a-Glucosidase and Amy-
lase(s) by Pseudomonas SB15 and a Mutant Strain. TOSHIYUKI SUGIMOTO,AKINORI AMEMURA, AND TOKUYA HARADA................................ 336
Radiometric Measurement of Metabolic Activity of Mycobacterium lepraemurium.EDWALDO E. CAMARGO, STEVEN M. LARSON, BYRON S. TEPPER, AND HENRYN. WAGNER, JR.......................................................... 452
Degradation of Oil Shale by Sulfur-Oxidizing Bacteria. J. FINDLEY, M. D. APPLE-MAN, AND T. F. YEN............. 460
Simple Method for Preparation of Homogeneous Spore Suspensions Useful in IndustrialStrain Selection. S. W. QUEENER AND J. J. CAPONE ..... ................... 498
Gas-Tight Flask for the Concurrent Measurement of Gas Metabolism and Growth inMethane-Oxidizing Bacteria. ELAINE F. MUNOZ AND MELVIN P. SILVERMAN... 507
Clinical Microbiology and ImmunologyCharacterization and Differentiation of 59 Strains of Moraxella urethralis from Clinical
Specimens. P. S. RILEY, D. G. HOLLIS, AND R. E. WEAVER................ 355Indirect Hemagglutinating Antibody Response to Herpesvirus hominis Types 1 and 2 in
Immunized Laboratory Animals and in Natural Infections of Man. ARTHUR F.BACK AND NATHALIE J. SCHMIDT.......................................... 392
Evaluation of the Gonosticon Dri Dot Test in Females with a Low Incidence ofGonorrhea. JOHN D. DYCKMAN, REUBEN D. WENDE, AND ROBERT P. WILLIAMS. 431
Comparison of the BACTEC System with Blind Subculture for the Detection ofBacteremia. M. CASLOW, P. D. ELLNER, AND T. E. KIEHN.................. 435
Effect of a Synthetic Dressing Formed on a Burn Wound in Rats: a Comparison of Allo-grafts, Collagen Sheets, and Polyhydroxyethylmethacrylate in the Control ofWound Infection. PAUL NATHAN, BRUCE G. MACMILLAN, AND IAN A. HOLDER .. 465
Survey on the Incidence of Yersinia enterocolitica Infection in Canada. S. TOMAAND LUCETTE LAFLEUR.................................................. 469
Origin and Structure of the Group-Specific, Complement-Fixing Antigen of Rickettsiarickettsii. T. TzIANABOS, E. L. PALMER, J. F. OBIJESKI, AND M. L. MARTIN.... 481
Contamination of Bacteriological Media by Leptospira biflexa. J. J. BRENDLE ANDA. D. ALEXANDER ...................................................... 505
Distribution of Serratia Species in Clinical Specimens. ERIOUS JOHNSON AND PAULD. ELLNER............................................................. 513
Modification of Tergitol-7 Agar for Differentiation of Hydrogen Sulfide-ProducingColonies. D. B. HINDS AND A. T. HOWARD................................ 521
Virology and Viral ImmunologyStability of Minute Virus of Mice to Chemical and Physical Agents. ROBERT E.
HARRIS, PHILIP H. COLEMAN, AND PAGE S. MORAHAN........................ 351Evaluation of a Plaque Assay for the Maedi-Progressive Pneumonia-Visna Viruses.
R. S. TROWBRIDGE...................................................... 366Typing Herpesvirus hominis Antibodies and Isolates by Inhibition of the Indirect
Hemagglutination Reaction. ARTHUR F. BACK AND NATHALIE J. SCHMIDT.... 400Improved Rapid Plate Method for the Isolation of Bacteriophages from Lysogenic
Bacteria. JOSEPH T. PARISI AND HENRY W. TALBOT, JR................... 503Determination of Genome Size of Selected Typing Bacteriophages of Staphylococcus
aureus. MICHAEL W. PARIZA AND JOHN J. IANDOLO ........................ 510Epstein-Barr Virus from P3HR-1 Cells Grown in Chemically Defined Medium.
STANLEY C. NAGLE AND BRUCE L. BROWN.................................. 518
DisinfectantsTemperature-Induced Changes in the Sporicidal Activity and Chemical Properties
of Glutaraldehyde. S. THOMAS AND A. D. RUSSELL ........................ 331
X1XCONTENTS
Volume 28 Contents for October Number 4
Environmental Microbiology and EcologyAutomated Count and Size Evaluation of Colonies of Bacteria Grown in a Zonal
Concentration Gradient of Antimicrobial Agent. E. YOURASSOWSKY ANDE. SCHOUTENS .......................................................... 525
Effect of Four Dispersants on Biodegradation and Growth of Bacteria on Crude Oil.G. J. MULKINS-PHILLIPS AND JAMES E. STEWART.......................547
Sorption of Heterotrophic and Enteric Bacteria to Glass Surfaces in the ContinuousCulture of River Water. CHARLES W. HENDRICKS.................... 572
Isolation of Ureolytic Peptostreptococcus productus from Feces Using Defined Medium;Failure of Common Urease Tests. V. H. VAREL, M. P. BRYANT, L. V. HOLDE-MAN, AND W. E. C. MOORE............................................... 594
Statistical Analysis of the Recovery of Coliform Organisms on Gelman andMilliporeMembrane Filters. DAVID J. SCHAEFFER, MAXINE C. LONG, AND KONANUR G.JANARDAN.............................................................. 605
Acetylene Reduction (Nitrogen Fixation) by Pulp and Paper Mill Effluents and byKlebsiella Isolated from Effluents and Environmental Situations. R. KNOWLES,R. NEUFELD, AND S. SIMPSON............................................. 608
Bacillus stearothermophilus in Soils of Iceland. M. L. FIELDS AND P. P. CHEN LEE.... 638Clostridium perfringens in the Environment. JACK R. MATCHES, JOHN LISTON, AND
DONALD CURRAN ............................................. 655Microbial Response to Drought in a Texas Highplains Shortgras§ Prairie. D. W.
THAYER................................................................ 700Isolation of Salmonella enteritidis Serotype Agona from Eutrophic Regions of a Fresh-
water Lake. W. L. COOK, R. A. CHAMPION, AND D. G. AHEARN............... 723
Food Microbiology and ToxicologyProduction of Patulin by Penicillium expansum. NOEL F. SOMMER, JACK R. Bu-
CHANAN, AND ROBERT J. FORTLAGE ........................................ 589Staphylococcal Enterotoxin B and Nuclease Production Under Controlled Dissolved
Oxygen Conditions. DAVID F. CARPENTER AND GERALD J. SILVERMAN........ 628Identification of Major High-Boiling Volatile Compounds Produced During Re-
frigerated Storage of Haddock Fillets. T. C. CHEN, W. W. NAWAR, AND R. E.LEVIN................................................................. 679
Taxonomic Significance of Phenethyl Alcohol Production by Achromobacter Isolatesfrom Fishery Sources. T. C. CHEN AND R. E. LEVIN........................ 681
Incidence of Clostridium botulinum in Crabmeat from the Blue Crab. DONALD A.KAUTTER, TIMOTHY LILLY, JR., ARMAND J. LEBLANC, AND RICHARD K. LYNT........ 722
Metabolism and ProductsBiology of Borrelia hermsii in Kelly Medium. HERBERT G. STOENNER ......... 540Production of Hormone B by Achlya heterosexualis. ALMA W. BARKSDALE AND LINDA
L. LASURE............................................................. 544Nalidixic Acid for Enrichment of Auxotrophs in Cultures of Salmonella typhimurium.
R. M. WEINER, M. J. VOLL, AND T. M. COOK.............................. 579Carotenoid Pigments of Mycobacterium kansasii. HUGO L. DAVID ........... 696
Clinical Microbiology and ImmunologyFlat Gel Polyacrylamide Electrophesis of Porcine Mycoplasmas. T. G. WREGHITT,
G. D. WINDSOR, AND M. BUTLER.......................................... 530Combined Biochemical and Serological Typing of Clinical Isolates of Klebsiella.
R. P. RENNIE AND I. B. R. DUNCAN ....................................... 534Non-Immunological Precipitation of Serum by Sodium Dodecyl Sulfate in Agar
Diffusion. C. T. CHO AND K. K. FENG.557
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Determination of Bacteroides melaninogenicus Serogroups by Fluorescent AntibodyStaining. DWIGHT W. LAMBE, JR......................................... 561
Monitoring the Atmosphere in an Anaerobic Chamber. SARA Z. SUDO AND PAULA. HERSCH......................... 582
Rapid Method of Determining Cholera Vibrio Biotype. P. MACKOWIAK AND IMBADULHuQ.................................................. ............. 586
Identification of Salmonella with the 0-1 Bacteriophage. SUSAN WELKOS, MINNIESchreiber, and Herman Baer.............................................. 618
Value of the Concomitant Use of Complement Fixation and Immunodiffusion Testsin the Diagnosis of Coccidioidomycosis. LEO KAUFMAN AND MAXINE J. CLARK.. 641
Comparison of Macroscopic, Microscopic, and Radiometric Examinations of ClinicalBlood Cultures in Hypertonic Media. RICHARD ROSNER.................... 644
Modified Laminar Flow Biological Safety Cabinet. GERALD J. MCGARRITY ANDLEWIS L. CORIELL...................................................... 647
Adhesive Tape: Potential Source of Nosocomial Bacteria. DAVID M. BERKOWITZ,WIE-SHING LEE, GEORGE J. PAZIN, ROBERT B. YEE, AND MONTO Ho.651
Evaluation of the Miitek System for Identification of Enterobacteriaceae. T. E.KIEHN, K. BRENNAN, AND P. D. ELLNER.................................. 668
Characterization of Klebsiella Isolates from Natural Receiving Waters and Compari-son with Human Isolates. JOHN M. MATSEN, JOYCE A. SPINDLER. AND RUSSELL0. BLOSSER............................................................ 672
Comparison of Media for the Isolation of Haemophilus Species from Cases of SeasonalConjunctivitis Associated with Sever Endemic Trachoma. DAVID W. VASTINE,CHANDLER R. DAWSON, ISAO HOSHIWARA, CHIEKO YONEDA, TOUFIQUE DAGH-FOUS, AND MOHAMMED MESSADI.688
Mathematical Analysis of the API Enteric 20 Profile Register Using a ComputerDiagnostic Model. E. ARTHUR ROBERTSON AND JAMES D. MACLOWRY. 691
Ultrastructure of the Surface of Rickettsia prowazeki and Rickettsia akari. ERSKINEL. PALMER, MARY L. MARTIN, AND LOUIS MALLAVIA ........................ 713
Acetamide Agar for Differentiation of Nonfermentative Bacteria. THOMAS R.OBERHOFER AND JOYCE W. ROWEN.. 720
Micro Cell Culture Method for Isolation of Chlamydia trachomatis. DOROTHY E.MCCOMB AND CONSTANCE I. PUZNIAK..................................... 727
Tube Culture Method for Viable Counts of Campylobacter fetus (Vibrio fetus).MARYON M. BORDER, B. D. FIREHAMMER, AND L. L. MYERS................. 730
Virology and Viral ImmunologyMicroadaptation of Hemadsorption Inhibition for Neutralization Tests with Pig
Hemagglutinating Encephalomyelitis Virus. K. J. S0RENSEN................ 553Rapid Typing of Herpes Simplex Virus Strains Using the Indirect Immunoperoxidase
Method. DENIS R. BENJAMIN ........................................... 568Specificity and Sensitivity of Radioimmunoassay for Hepatitis B Antigen. GILBERT
R. IRWIN, ALFRED M. ALLEN, WILLIAM H. BANCROFT, MILTON WILLHIGHT, ANDPHILIP K. RUSSELL..................................................... 600
Sensitive In Vivo Assay for Detection of Murine Leukemia Viruses. ROBERT L.PETERS, GERARD J. SPAHN, LOUISE S. RABSTEIN, ROBERT J. HUEBNER, ANDGARY J. KELLOFF.. 614
Serotyping of Adnoviruses Using Immune Electron Microscopy. JOHN H. VASSALLII AND C. GEORGE RAY.................................................. 623
Solid Phase Radioimmunoassay for Identification of Herpesvirus hominis Types 1 and2 from Clinical Materials. BAGHER FORGHANI, NATHALIE J. SCHMIDT, ANDEDWIN H. LENNETrE.................................................... 661
Concentration of Enteric Viruses from Water with Lettuce Extract. J. KONOWAL-CHUK, J. I. SPEIRS, R. D. PONTEFRACT, AND G. BERGERON ................... 717
Ehrlich Ascites Tumor Preservation for Fifteen Years -a Simple Method. WILLIAMA. CASSEL AND KATHY M. MCCASKILL.726
Penicillium stoloniferum Virus: Large-Scale Concentration and Purification by Poly-ethylene Glycol. R. W. DETROY AND P. E. STILL.733
xxiCONTENTS
MethodsSpecimen Holder to Critical-Point Dry Microorganisms for Scanning Electron Micros-
copy. C. P. KURTZMAN, F. L. BAKER, AND J. J. SMILEY....................... 708
Volume 28 Contents for November Number 5
Environmental Microbiology and EcologyTest Method for the Evaluation of Virucidal Efficacy of Three Common Liquid Sur-
face Disinfectants on a Simulated Environmental Surface. J. W. GAUSTAD, C. R.MCDUFF, AND H. J. HATCHER............................................ 748
Effects of Seawater Cations and Temperature on Manganese Dioxide-ReductaseActivity in a Marine Bacillus. W. C. GHIORSE AND H. L. EHRLICH............. 785
Fermentation of Feedlot Waste Filtrate by Fungi and Streptomycetes. B. A. WEINERAND R. A. RHODES...................................................... 845
Direct Method for Continuous Determination of Iron Oxidation by Autotrophic Bac-teria. MICHAEL STEINER AND NORMAN LAZAROFF.......................... 872
Rickettsia-Like Organism Causing Disease in a Crangonid Amphipod from Florida.BRIAN A. FEDERICI, EDWIN I. HAZARD, AND DARRELL W. ANTHONY............ 885
Simplified Method for the Preparation of Silica Gel Media. ROBERT C. THATCHERAND TERRY L. WEAVER.................................................. 887
Persistence of Enteroviruses in Lake Water. JOHN E. HERRMANN, KENNETH D.KOSTENBADER, JR., AND DEAN 0. CLIVER.................................. 895
Food Microbiology and ToxicologyCharacterization of Lac+ Transductants of Streptococcus lactis. T. A. MOLSKNESS,
W. E. SANDINE, AND L. R. BROWN........................................ 753Aspergillusflavus Infection and Aflatoxin Production in Corn: Influence of Trace Ele-
ments. E. B. LILLEHOJ, W. J. GARCIA, AND M. LAMBROW................... 763Recovery of Heated Clostridium perfringens Type A Spores on Selective Media. J. T.
BARACH, D. M. ADAMS, AND M. L. SPECK.................................. 793Response of Ligated Intestinal Loops in Chickens to the Enterotoxin of Clostridium
perfringens. L. NIILO................................................... 889
Metabolism and ProductsIdentification of n-Decane Oxidation Products in Corynebacterium Cultures by
Combined Gas Chromatography-Mass Spectrometry. PAOLO BACCHIN, ANDREAROBERTIELLO, AND AURELIO VIGLIA....................................... 737
Submerged Production, Purification, and Crystallization of Acid Carboxypeptidasefrom Penicilliumjanthinellum IFO-8070. SADAJI YOKOYAMA, AKIRA OOBAYASHI,OSAMU TANABE, AND EIJI ICHISHIMA...................................... 742
Cold Shock Lethality and Injury in Clostridium perfringens. P. A. TRACI AND C. L.DUNCAN ............................................................... 815
Diauxic Growth of Propionibacterium shermanii. INN HEE LEE, A. G. FREDRICKSON,AND H. M. TSUCHIYA............................................... .....831
Toxicity of Short-Chain Fatty Acids and Alcohols Towards Cladosporium resinae.J. S. TEH.............................................................. 840
Growth of Bacteroides fragilis in Continuous Culture and in Batch Cultures at Con-trolled pH. ELI DALLAND AND T. HOFSTAD................................ 856
Formation of Diketopiperazines by Penicillium italicum Isolated from Oranges.PETER M. SCOTT, BARRY P. C. KENNEDY, JOOST HARWIG, AND Y-K. CHEN...... 892
Clinical Microbiology and ImmunologyRapid Methods for Biochemical Testing of Anaerobic Bacteria. PAUL C. SCHRECKEN-
BERGER AND DONNA J. BLAzEvIC ......................................... 759
*xii CONTENTS
Differentiation of Staphylococcal and Micrococcal Proteinases by Electrophoresis.R. K. SCHERER AND R. W. BROWN........................................ 768
Parabiotic Chamber for Organ Cultures: Improved Model. MICHAEL G. GABRIDGE .. 774In-Use Contamination of Intravenous Infusion Fluid. DENNIS G. MAKI, ROGER L.
ANDERSON, AND JONAS A. SHULMAN....................................... 778Evaluation of the BBL Minitek System for the Identification of Enterobacteriaceae.
S. L. HANSEN, D. R. HARDESTY, AND B. M. MYERS.798Corynebacterial Kidney Disease of Salmonids: Growth and Serological Studies on the
Causative Bacterium. G. L. BULLOCK, H. M. STUCKEY, AND P. K. CHEN. 811Factors That Influence Microbial Contamination of Fluids Associated with Hemo-
dialysis Machines. MARTIN S. FAvERo, LORETTA A. CARSON, WALTER W. BOND,AND NORMAN J. PETERSEN.822
Simplified Extraction Procedure for Serological Grouping of Beta-Hemolytic Strepto-cocci. AzIz EL KHOLY, RICHARD M. KRAUSE, AND LEWIS W. WANNAMAKER 836
Fluorescent Antibody Study of the Gram-Positive Anerobic Cocci. RICHARD K.PORSCHEN AND EARLE H. SPAULDING.851
Investigations on the Specificity of the Limulus Test for the Detection of Endotoxin.ALEXANDER WILDFEUER, BERNO HEYMER, KARL H. SCHLEIFER, AND OTTOHAFERKAMP.867
Rapid Detection of Bovine Mycoplasma Antigens by Counterimmunoelectrophoresis.HYUN J. CHO AND EDGAR V. LANGFORD.897...............................897
Virology and Viral ImmunologyMicroculture Plaque Neutralization Test for California-Group Arboviruses. GARY L.
SEAWRIGHT, GHERRY HARDING, FREDERICK C. THOMAS, AND ROBERT P. HANSON. 802Diagnosis of California (La Crosse) Encephalitis by Precipitin Techniques: a Prospec-
tive Study. HENRY H. BALFOUR, JR., AND CHARLENE K. EDELMAN.807Virulence of Venezuelan Equine Encephalomyelitis Virus Subtypes for Various Lab-
oratory Hosts. CHARLES H. CALISHER AND KATHRYN S. C. MANESS.......... 881
DisinfectantsChemical Disinfection of Holding-Tank Sewage. MARK D. SOBSEY, CRAIG WALLIS,
AND JOSEPH L. MELNICK.861
Volume 28 Contents for December Number 6Environmental Microbiology and Ecology
Effect of Environmental Parameters on Bacterial Degradation of Bunker C Oil,Crude Oils, and Hydrocarbons. G. J. MULKINS-PHILLIPS AND JAMES E. Stew-art.915
Modified Spinning Top Homogeneous Spray Apparatus for Use in ExperimentalRespiratory Disease Studies. HAROLD W. YOUNG, EDGAR W. LARSON, ANDJOSEPH W. DOMINIK............................... ............. 929
Arthrobadter globiformis and Its Bacteriophage in Soil. L. E. CASIDA, JR., ANDKANG-CHIENLiu. 951
Microbial Metabolism and Dynamic Changes in the Electrical Conductivity of SoilSolutions: a Method for Detecting Extraterrestrial Life. MELVIN P. SILVERMANAND ELAINE F. MUNOZ .................................................. 960
Effect of Hydrostatic Pressure on Growth and Viability of Vibrio parahaemolyticus.J. R. SCHWARZ AND R. R. COLWELL ..977
Deep-Sea Bacteria: Growth and Utilization of Hydrocarbons at Ambient and In SituPressure. J. R. SCHWARZ, J. D. WALKER, AND R. R. COLWELL ..982
Agricultural Plants and Soil as a Reservoir for Pseudomonas aeruginosa. SYLVIA K.GREEN, MILTON N. SCHROTH, JOHN J. CHO, SPYROS D. KOMINOS, AND VILMA B.VITANZA-JACK.......................................................... 987
*x*iaCONTENTS
Biodegradation of Cyanuric Acid. JEROME SALDICK............................. 1004Distribution of Vibrio parahaemolyticus and Related Organisms in the Atlantic Ocean
off South Carolina and Georgia. TATSUO KANEKO AND RITA R. COLWELL.... 1009Nutritional Features of the Intestinal Anaerobe Ruminococcus bromii. JANICE L.
HERBECK AND M. P. BRYANT................................. ......... 1018Membrane Concentration of Infectious Bovine Rhinotracheitis Virus from Water.
S. R. TsCHIDER, D. L. BERRYHILL, AND I. A. SCHIPPER................... 1030Ecological Application of Antibiotics as Respiratory Inhibitors of Bacterial Popula-
tions. J. E. YETEA AND W. J. WIEBE................................... 1033Growth and Reproduction of Microorganisms Under Extremely Alkaline Conditions.
K. A. SOUZA, P. H. DEAL, H. M. MACK, AND C. E. TURNBILL........... 1066Method for In Situ Measurement of Nitrification in a Stream. DAVID P. SCHWERT
AND JAMES P. WHITE................................................... 1082Microbial Degradation of High-Molecular-Weight Alkanes. J. R. HAINES AND M.
ALEXANDER ........................................................... 1084Evaluation of Selective Media for Enumeration of Group D Streptococci in Bovine
Feces. R. W. SWITZER AND JAMES B. EVANS............................. 1086
Food Microbiology and ToxicologyPurification of Clostridium botulinum Type F Progenitor Toxin. IWAO OHISHI AND
GENJI SAKAGUCHI ....................................................... 923Detecting the Enterotoxicity of Staphylococcus aureus strains. RUTH ROBBINS,
SARA GOULD, AND MERLIN BERGDOLL.................................... 946Further Characterization of Tissue Distribution and Metabolism of [49C]Aflatoxin
B1 in Chickens. JOHN R. CHIPLEY, MICHAEL S. MABEE, KENNETH L. APPLE-GATE, AND MARK S. DREY USS ...........................................1027
Periplasmic Structure in Saccharomyces rouxii (Boutroux), an Osmophil. WILFREDN. ARNOLD, ROBERT G. GARRISON, AND KAREN S. BOYD.................. 1047
Technique for Preparing High-Quality Microphotographs by Fluorescence Micros-copy. J. G. ELLIOT, J. A. CARPENTER, AND M. K. HAMDY ................... 1063
Recovery of Lactobacillus bulgaricus and Streptococcus thermophilus on Nine CommonlyUsed Agar Media. NANCY J. MOON, A. C. HAMANN, AND G. W. REINBOLD.. 1076
Antagonism of Lactic Streptococci Toward Staphylococcus aureus in AssociativeMilk Cultures. S. E. GILLILAND AND M. L. SPECK......................... 1090
Toxigenic Aspergilli and Penicillia Isolated from Aged, Cured Meats. M. T. Wu,J. C. AYRES, AND P. E. KOEHLER......................................... 1094
Metabolism and ProductsContinuous Culture Used for Media Optimization. R. I. MATELES AND E. BATTAT. 901Growth of Pseudomonas C on C1 Compounds: Continuous Culture. E. BATTAT, I.
GOLDBERG, AND R. I. MATELES .......................................... 906Microbial Transformation of Methyl 5(6)-Butyl-2-Benzimidazolecarbamate. J. R.
VALENTA, C. J. DICUOLLO, L. R. FARE, J. A. MILLER, AND J. F. PAGANO..... 995Culture of Clostridium pasteurianum in Defined Medium and Growth as a Function
of Sulfate Concentration. M. F. MALLETTE, PAUL REECE, AND E. A. DAWES. 999Quantitation of the Tetramethyl-p-Phenyletediamine Oxidase Reaction in Neisseria
Species. PETER JURTSHUK AND THOMAS W. MILLIGAN.................... 1079Comparison of the Sphingolipid Content of Rumen Bacteroides Species. JOSEPH E.
KUNSMAN AND DANIEL R. CALDWELL.................................... 1088
Clinical Microbiology and ImmunologyGrowth of Chlamydia trachomitis in McCoy Cells Treated with Cytochalasin B.
DAVID SOMPOLINSKY AND SHIRLEY RICHMOND ............................. 912Criteria for Preparing, Evaluating, and Standardizing lodinated Globulins for Radio-
immunoassay Procedures. HARRIET D. HUTCHINSON AND DONALD W. ZIEGLER. 935Primary Isolation of Mycobacterium chelonei Subspecies abscessus from Pus Inoculated
xxiv CONTENTS
into Peptone Broth. JULIA CROWLEY, PAUL I. Liu, AND ARMAND B. GLASSMAN. 943New Method of Grouping Beta-Hemolytic Streptococci Directly on Sheep Blood
Agar Plates by Coagglutination of Specifically Sensitized Protein A-ContainingStaphylococci. E. A. EDWARDS AND G. L. LARSON........................ 972
Factors Affecting the Sensitivity of Limulws Lysate. JAMES D. SULLIVAN, JR., ANDSTANLEY W. WATSON................................................... 1023
Observation of Nitrate Reduction in Some Non-Saccharolytic Strains of Acinetobacter.P. S. RILEY AND R. E. WEAvER ........................................ 1071
Latex Test for Quantitative Determination of Escherichia coli Antibody. KARIMHECHEMY, ROY STEvENS, JEAN SROKA, AND HASSAN GAAFAR.............. 1073
Virology and Viral ImmunologyAutomated Microtransfer Technique for the Assay of Poliovirus- and Mumps Virus-
Neutralizing Antibodies. RONALD E. MAYNER, DONALD J. McDoRMAN, BAR-BARA C. MEYER, AND PAUL D. PARKMAN................................ 968
Rubella Hemagglutination Inhibition: Removal of Nonspecific Agglutination Dueto Manganous Chloride. S. A. BIANO, TE WEN CHANG, AND J. B. DANIELS.. 992
In Vitro System for Production of Mouse Mammary Tumor Virus. D. L. FINE,L. O. ARTHUR, J. K. PLOWMAN, E. A. HILLMAN, AND F. KLEIN............ 1040
Growth of Foot-and-Mouth Disease Virus in the Different Layers of Bovine Omasumin Suspended Cultures. BEN-AMI PELEG AND NILY RON................... 1055
DisinfectantsPropylene Oxide as Sterilizing Agent. A. HART AND M. W. BROWN............. 1069
D[VCONTENTS