atmospheric solids analysis probe - waters corporation · 2012-01-10 · inserting the inner probe...

Atmospheric Solids Analysis Probe

Operator’s Guide Supplement

Revision H

Copyright © Waters Corporation 20092011All rights reserved

Copyright notice

© 20092011 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use.

Trademarks

ESCi, SYNAPT, and Waters are registered trademarks of Waters Corporation, and LCT Premier, Lockspray, MassLynx, Q-Tof Premier, “THE SCIENCE OF WHAT’S POSSIBLE.”, and Xevo are trademarks of Waters Corporation.

Krytox is a registered trademark of E. I. du Pont de Nemours and Company.

PEEK is a trademark of Victrex Corporation.

Other registered trademarks or trademarks are the sole property of their owners.

ii

Customer comments

Waters Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at [email protected].

Contacting Waters

Contact Waters® with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization’s safety representative for guidance.

Waters contact information

Contacting medium Information

Internet The Waters Web site includes contact information for Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-HPLC, or fax 508 872 1990.For other locations worldwide, phone and fax numbers appear in the Waters Web site.

Conventional mail Waters Corporation34 Maple StreetMilford, MA 01757USA

iii

Considerations specific to the ASAP

High temperature hazard

Puncture hazard

Gas leakage hazard

Sample leakage hazard

Safety advisories

Consult Appendix A for a comprehensive list of warning and caution advisories.

Warning: To avoid burn injuries, avoid touching the probe tip or probe sheath of the ASAP directly.

Warning: To avoid puncture injuries, do not handle broken capillary ends. Glass capillaries can break easily.

Warning:

• To avoid excessive gas escaping into the work environment, do not hold the ASAP’s sealing lever open during instrument operation.

• Do not use the ASAP if the sealing lever spring clamping mechanism appears loose or defective.

• The instrument source exhaust system must not be closed or impeded.

• Blank off the instrument nebulizing gas outlet on the front panel with the supplied blanking plug when ASAP is fitted, to avoid excessive gas escaping into the work environment.

Warning: The ASAP is not completely leak proof, and you must undertake an appropriate hazard analysis when analyzing toxic samples.

iv

Operating this device

When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section.

Applicable symbols

Audience and purpose

This guide presents installation, operation, and safety information for an atmospheric solids analysis probe (ASAP) used with compatible instruments.

Intended use of the ASAP

Waters designed the ASAP to allow rapid, direct analysis of samples using the atmospheric pressure ionization (API) source on compatible instruments. The ASAP is for research use only and is not intended for use in diagnostic applications. It is intended for use only by qualified laboratory personnel, installation engineers, and field service engineers.

Symbol Definition

Manufacturer location

Authorized representative of the European Community

Confirms that a manufactured product complies with all applicable European Community directives

Australia C-Tick EMC compliant

Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements

Consult instructions for use

v

ISM classification

ISM Classification: ISM Group 1 Class A

This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class A products are suitable for use in commercial (that is, nonresidential) locations and can be directly connected to a low voltage, power-supply network.

EC authorized representative

Waters Corporation (Micromass UK Ltd.)Floats RoadWythenshaweManchester M23 9LZUnited Kingdom

Telephone: +44-161-946-2400

Fax: +44-161-946-2480

Contact: Quality manager

vi

Table of Contents

Copyright notice ................................................................................................... ii

Trademarks ............................................................................................................ ii

Customer comments ............................................................................................ iii

Contacting Waters ............................................................................................... iii

Safety considerations .......................................................................................... iii Considerations specific to the ASAP.................................................................. iv Safety advisories ................................................................................................. iv

Operating this device ........................................................................................... v Applicable symbols .............................................................................................. v Audience and purpose.......................................................................................... v Intended use of the ASAP ................................................................................... v

ISM classification ................................................................................................. vi ISM Classification: ISM Group 1 Class A ......................................................... vi

EC authorized representative ........................................................................... vi

1 The Atmospheric Solids Analysis Probe (ASAP) Option .............. 1-1

Introduction and overview ............................................................................. 1-2

ASAP operating principles ............................................................................. 1-3

Identifying the device parts ........................................................................... 1-4

Installing the outer assembly ......................................................................... 1-6

Inserting the inner probe ................................................................................ 1-7

2 Using the ASAP ....................................................................................... 2-1

Preparing the ASAP for use ........................................................................... 2-2 Calibration ....................................................................................................... 2-2 Optimizing the probe position......................................................................... 2-2 Baking the source ............................................................................................ 2-2

Table of Contents vii

Loading samples ................................................................................................ 2-3 Solid samples ................................................................................................... 2-3 Liquid samples ................................................................................................. 2-3 Samples in solution.......................................................................................... 2-4

Analyzing samples ............................................................................................ 2-4 Preparing to acquire data................................................................................ 2-4 Acquiring data.................................................................................................. 2-5 Ionization mechanisms.................................................................................... 2-7 Analyzing non-polar compounds ..................................................................... 2-8 Thermal fragmentation ................................................................................. 2-10 Using modifiers .............................................................................................. 2-11 Instrument methods ...................................................................................... 2-12

A Safety Advisories .................................................................................. A-1

Warning symbols ............................................................................................... A-2 Task-specific hazard warnings........................................................................ A-2

Caution symbol .................................................................................................. A-3 Specific warnings ............................................................................................. A-3

Warnings that apply to all Waters instruments ......................................... A-5

Electrical and handling symbols ................................................................... A-7 Electrical symbols ............................................................................................ A-7 Handling symbols ............................................................................................ A-8

viii Table of Contents

1 The Atmospheric Solids Analysis Probe (ASAP) Option

This chapter provides an overview of the ASAP and detailed instructions for installation on compatible instruments.

Contents:

Topic Page

Introduction and overview 1-2

ASAP operating principles 1-3

Identifying the device parts 1-4

Installing the outer assembly 1-6

Inserting the inner probe 1-7

1-1

Introduction and overview

The ASAP is an optional device used with compatible instruments. It facilitates rapid analysis of volatile and semi-volatile compounds in solids, liquids, and polymers. It is particularly suited to analyzing low-polarity compounds.

The ASAP directly replaces the electrospray or APCI probe in the instrument’s source housing and has no external gas or electrical connections.

The ASAP is compatible with the following instruments:

* These instruments require the Xevo version of the ASAP.

• LCT Premier™ XE • Xevo TQ MS* • SYNAPT G2 MS*

• SYNAPT® • TQ Detector • SYNAPT G2 HDMS*

• Q-Tof Premier™ • SQ Detector • Xevo G2 QTof*

• Xevo™ QTof MS* • 3100 Mass Detector • Xevo G2 Tof*

• Xevo TQ-S* • Xevo TQD* • SQ Detector 2*

1-2 The Atmospheric Solids Analysis Probe (ASAP) Option

ASAP operating principles

Illustration of ASAP technique:

The ASAP technique depends on heated nitrogen desolvation gas to vaporize the sample and corona discharge for ionization. It can ionize low-polarity compounds not amenable to electrospray (ESI), atmospheric pressure chemical ionisation (APCI), and atmospheric pressure photoionization (APPI), at high sensitivity. You can also use the technique to analyze complex samples, without needing to extensively prepare them.

The ASAP device is designed to modify an existing atmospheric pressure ionization (API) source, and you can use it as a substitute for electron-ionization (EI) or chemical-ionization (CI) vacuum, solids-probe analyses.

MS inlet

Heated gas

ASAP

Glass capillary

Corona discharge

ASAP operating principles 1-3

Identifying the device parts

ASAP parts identification:

Inner probe

Outer assembly

Glass capillary

Probe sheath

Adjuster knob

Probe tip

Sealing lever

Attachment thumbscrews

Body cover

Spring clip


ASAP parts identification (Xevo version):

Inner probe

Outer assembly

Glass capillary

Probe sheath

Probe grip

Probe tip

Sealing lever

Body cover

Spring clipLocking ring

Identifying the device parts 1-5

Installing the outer assembly

Requirement: Calibrate the instrument before you fit the ASAP. To do so, follow the calibration procedures in the MassLynx online Help (see page 2-4).

To install the outer assembly:

1. Remove any existing probe from the source housing, see the instrument’s Operator’s Overview and Maintenance Guide.

2. Where applicable, blank off the nebulizing gas outlet on the front panel using the supplied blanking plug.

3. Fit an APCI corona pin into the source, adjusting it to point to the sample cone.

Requirement: If you are using the LockSpray interface, use the supplied ESCi® corona pin to avoid making contact with the lock-spray baffle.

4. Holding the outer assembly by the body cover, fit it into the source housing and secure it with the attachment thumbscrews.

Tip: The Xevo version of the probe does not have thumbscrews. To secure it, rotate the locking ring clockwise until tight.

Result: The outer assembly is now ready to accept the inner probe.

Warning: For instruments with a nebulizing gas outlet on their front-panel, to avoid excessive gas escaping into the work environment, blank off the instrument nebulizing gas outlet.


Inserting the inner probe

Refer to the figure on page 1-8 while following this procedure. If you are using the Xevo version of the probe, refer to the figure on page 1-9.

To insert the probe:

1. Depress and hold down the spring clip on the inner probe.

2. Insert one of the supplied glass capillaries as far as possible into the probe tip.

Tip: Avoid handling the capillary end to reduce contamination.

3. Release the spring clip.

Requirement: Ensure the capillary is held firmly in place before proceeding.

4. Depress and hold the sealing lever on the outer assembly to expose the central cavity.

5. Insert the inner probe fully into the central cavity, making sure the capillary end does not contact any part of the outer assembly.

6. Release the sealing lever.

Warning: To avoid burn injuries, avoid touching the probe tip or probe sheath.


Warning: To avoid excessive gas escaping into the work environment, do not hold the ASAP’s sealing lever open during instrument operation.


Inserting the inner probe:


Inserting the inner probe (Xevo version):


2 Using the ASAP

This chapter explains the ASAP’s operation with compatible instruments.

Contents:

Topic Page

Preparing the ASAP for use 2-2

Loading samples 2-3

Analyzing samples 2-4

2-1

Preparing the ASAP for use

Calibration

Normal practice is to calibrate the instrument before you fit the ASAP. To do so, follow the calibration procedures in the MassLynx online Help.

However, if your source contains a lock-spray reference probe, you can calibrate the instrument after fitting the ASAP by using the reference probe to introduce the calibration reference solution.

A suitable calibration compound for all compatible instruments is sodium formate.

Optimizing the probe position

You can optimize the position of the capillary end relative to the sample cone and corona pin by adjusting the micrometer on the source housing. A good starting position for the capillary end is mid-way between the two.

You can move the capillary end closer to the sample cone and corona pin by rotating the adjuster knob of the inner probe, ensuring that the capillary end clears the rotating lock-spray baffle (if fitted). If the baffle is not fitted, you can move the capillary end closer to the sample cone and corona pin to achieve better sensitivity. In this case, use the APCI corona pin supplied with your instrument.

You can also make fine adjustments to the probe position as the sample vaporizes, using both controls while monitoring the sample intensity of a suitable compound in the MassLynx Tune window. Once you find the optimal position, you can use it when analyzing subsequent samples.

Tip: You cannot adjust the probe position when using the Xevo version of the probe. It is designed such that the capillary end is already in the correct position for optimal sensitivity.

Baking the source

Because the ASAP technique is highly sensitive, you should “bake out” the source (with the inner probe fitted) after installing it on an instrument.

2-2 Using the ASAP

After the first installation, bake the source overnight using the settings shown below. On subsequent installations, baking for one hour reduces the presence of background ions to a minimum.

See page 2-7 for details on the importance of baking out.

Loading samples

Load samples onto the exposed end of a sealed glass capillary before fitting the capillary into the inner probe (see page 1-7).

Solid samples

Load the sample by rubbing the capillary gently across the surface of the solid, removing excess sample with a tissue.

Note, however, that using a tissue creates a background spectrum. You can compensate for this unwanted effect by obtaining a separate spectrum from the tissue and subtracting it from the sample spectrum. Alternatively, in an extraction hood, blow the excess sample from the capillary end using a stream of nitrogen.

Liquid samples

Load the sample by dipping the capillary end in the liquid, removing as much liquid as possible with a tissue.

Note, however, that using a tissue creates a background spectrum. You can compensate for this unwanted effect by obtaining a separate spectrum from the tissue and subtracting it from the sample spectrum. Alternatively, in an extraction hood, blow the excess sample from the capillary end using a stream of nitrogen.

Recommended bake-out settings:

Source Temp 150 °C

Desolvation Gas Flow 1200 L/h

Desolvation Gas Temp 400 to 500 °C


Loading samples 2-3

Samples in solution

Follow the procedure for liquid samples, or apply a 1-µL amount to the capillary end using a syringe or pipette.Use a fresh capillary to reduce contamination. If doing so is not practical, heating to 500 ºC will clean the capillary for most volatile and semi-volatile samples. You can reduce cooling time by operating the instrument with a desolvation gas flow of 1200 L/h and gas temperature of 50 ºC.

Analyzing samples

Preparing to acquire data

Before acquiring data from a loaded sample, set your instrument to operate in ESCi mode. To do so, in the MassLynx Tune window, click Ion Mode > ESCi.

The starting temperature for the desolvation gas heater is sample-dependent, although a temperature between 50 ºC and 100 ºC generally applies. When the heater reaches the starting temperature, you can vaporize the sample by gradually increasing the temperature, separating the mixtures by boiling point. If obtaining a vaporization profile of the sample is unnecessary, you can set a higher temperature. Although temperatures from 100 ºC to 350 ºC are typical, you can nevertheless specify higher temperatures for less volatile samples. Note, however, that higher temperatures can require increasing the lock mass reference flow rate to improve the stability of the reference peak.

Tip: The desolvation temperature readback value displayed in the Tune window measures temperature only at the desolvation heater block. The actual desolvation gas temperature around the capillary end depends on the desolvation gas flow rate, the heater temperature selected, and the probe position.

Recommended starting parameters

Parameter Value

Source temperature 120 ºC

Sample cone 30 V (requires tuning for optimum sensitivity)

Corona current 5 µA (requires tuning for optimum sensitivity)

Desolvation gas flow rate 500 to 1200 L/h

Desolvation gas heater 50 to 450 ºC (sample dependent)

2-4 Using the ASAP

Acquiring data

To ensure good results and obtain exact mass measurements throughout the vaporization profile of the sample, begin an acquisition before inserting the inner probe. Doing so allows you to acquire reference scans before introducing the sample.

Acquire data via the sample list with the Tune window open (refer to the MassLynx online Help for detailed instructions). Also create a method suitable for your instrument. See page 2-12 for examples of method parameters for each compatible instrument.

Once you insert the inner probe, increase the desolvation temperature to vaporize the sample. During an acquisition, you can manually change the temperature. When you complete the acquisition, the desolvation temperature returns to the value set in the Tune window.

Tip: For the LCT Premier XE, with lock spray enabled, when you withdraw the inner probe during the acquisition, the desolvation temperature returns to the start value set in the Tune window. Using this technique, you can save multiple analyses to a single data file.

As the instrument operates in ESCi mode, sample data are acquired in APCI mode, and lock-spray reference data (for lock-mass correction) are acquired in ESI mode. Both data types appear in the Tune window.


Tune window on LCT Premier XE:

2-6 Using the ASAP

Ionization mechanisms

In the ASAP technique, ion generation in positive-ion mode comes about by corona discharge, forming both radical cations (M+•) and protonated cations (M+H)+. Source conditions greatly affect the ions' state. A dry source—one that underwent overnight baking and to which no lock reference is introduced—favors the formation of radical cations. A source that is not dry favors the formation of protonated cations because it contains water vapor or deliberately introduced reference solution. The presence of water or other solvents in the source can severely affect the sensitivity of many nonpolar species, which tend to form radical cations.

The following examples illustrate the different ionization mechanisms, using progesterone as the sample.

Ionization by charge transfer:

This example uses a background ion for lock mass correction. The principal ion generated is the radical cation at m/z 314, but evidence of the protonated molecular ion also exists. The peak at m/z 315 is bigger than expected for the carbon 13C isotope alone because of traces of water vapor in the source.

Calculated mass for radical cationM+• = 314.2241


Ionization by proton transfer:

In this example, the protonated molecule appears as the major species, with little evidence of radical cations. The same sample and source conditions used in the previous example were used in this one, except that a lock reference solution was introduced, at 2 µL/min. Note that the degree of protonation also depends on the proton affinity of the analyte molecule. High proton affinity favors the formation of the protonated molecule.

Analyzing non-polar compounds

The ASAP technique particularly suits analyzing nonpolar compounds. See the figures on page 2-9, which show the results obtained from hexachlorobenzene (C6Cl6), which is normally done using the EI/CI technique under vacuum conditions.

In the following examples, the sample is introduced directly into the source. Because of the source chamber’s dry nitrogen atmosphere, radical cations form rather than the protonated ones (see page 2-7).

Calculated mass for protonated molecule(M+H)+ = 315.2324

2-8 Using the ASAP

Predicted and actual spectra (hexachlorobenzene):

The example shows the predicted isotope model for hexachlorobenzene as compared with the actual spectrum acquired using the ASAP technique. Comparison of the two spectra shows that they are a very close match, indicating the usefulness of the ASAP in analyzing compounds that normally require analysis with a vacuum solids probe technique.

Hexachlorobenzene (predicted)

Hexachlorobenzene (actual)


Thermal fragmentation

Controlling the temperature of the desolvation gas when analyzing samples significantly changes the appearance of the resulting spectrum because thermal fragmentation occurs at higher temperatures. The following example illustrates this effect using the hydrocarbon tetracontane (C40H82) as the sample.

Thermal fragmentation (tetracontane):

In this example, the lower spectrum shows the formation of the radical cation at m/z 562.6 when the temperature reaches 100 ºC. The upper spectrum acquired at a temperature of 250 ºC clearly shows that thermal fragmentation occurred.

Temperature = 250 ºC

Temperature = 100 ºC

2-10 Using the ASAP

Using modifiers

You can use to your advantage the tendency to form either protonated or solvated cations during an ASAP analysis. The following example shows how an analysis of Krytox® (a perfluorinated synthetic lubricant) produces different spectra under dry conditions versus the separate addition of methanol and ammonium hydroxide. Adding the modifiers generates solvent adduct ions (M+CH3OH+H)+ or (M+NH4)

+ of the polymer species, which simplifies the spectrum and aids in determining molecular weight.

Analysis of Krytox using modifiers:

Recommendation: You can introduce modifiers into the source by placing an unstoppered vial of the modifier inside the source chamber or infusing the modifier directly via the LockSpray interface.

Methanol

Ammonium Hydroxide

Nitrogen (dry)


Instrument methods

The following screen captures illustrate the method parameters to use for each compatible instrument when analyzing samples with the ASAP. The exact appearance of these screens can change depending on your software version.

Method parameters for LCT Premier XE:

2-12 Using the ASAP

Method parameters for Q-Tof Premier:

Acquisition tab

TOF MS tab


Method parameters for Q-Tof Premier (continued):

Collision Energy tab

Lock Mass tab

2-14 Using the ASAP

Method parameters for SYNAPT:

Acquisition tab

TOF MS tab


Method parameters for SYNAPT (continued):

Transfer CE Control tab

Trap CE Control tab

Lock Mass tab

2-16 Using the ASAP

Method parameters for Xevo TQ, Xevo TQD, and SQ Detector 2:

Method parameters for Xevo QTof:


Method parameters for SQ, TQ, and 3100 detectors:

2-18 Using the ASAP

Method parameters for SYNAPT G2 MS and HDMS:

Acquisition tab

TOF MS tab


Method parameters for SYNAPT G2 MS and HDMS (continued):

Trap CE Control tab

Transfer CE Control tab

2-20 Using the ASAP

Method parameters for Xevo G2 QTof and Xevo G2 Tof:

Method parameters for Xevo TQ-S:


2-22 Using the ASAP

A Safety Advisories

Waters® instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This appendix presents all the safety symbols and statements that apply to the entire line of Waters products.

Contents:

Topic Page

Warning symbols A-2

Caution symbol A-3

Warnings that apply to all Waters instruments A-5

Electrical and handling symbols A-7

A-1

Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument’s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution.

Task-specific hazard warnings

The following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others.

When the following symbols appear in a manual’s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of sharp-point puncture injury.)

Warning: (Risk of hand crush injury.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

A-2 Safety Advisories

Caution symbol

The caution symbol signifies that an instrument’s use or misuse can damage the instrument or compromise a sample’s integrity. The following symbol and its associated statement are typical of the kind that alert you to the risk of damaging the instrument or sample.

Specific warnings

The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts.

Biohazard warning

This warning applies to Waters instruments that can be used to process material that might contain biohazards: substances that contain biological agents capable of producing harmful effects in humans.

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Caution: To avoid damage, do not use abrasives or solvents to clean the instrument’s case.

Warning: Waters instruments and software can be used to analyze or process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials. To avoid infection with these agents, assume that all biological fluids are infectious, observe Good Laboratory Practices, and consult your organization’s biohazard safety representative regarding their proper use and handling. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).

Caution symbol A-3

Chemical hazard warning

This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material.

Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practices (GLP), and consult your organization’s safety representative regarding proper use and handling. Guidelines are provided in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals.


Warnings that apply to all Waters instruments

When operating this device, follow standard quality control procedures and the equipment guidelines in this section.

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.

注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設備的權利。

注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操作该设备的合法性。

주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

注意：規制機関から明確な承認を受けずに本装置の変更や改造を行うと、本装置のユーザーとしての承認が無効になる可能性があります。

Warnings that apply to all Waters instruments A-5

Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。

경고: 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다.

警告：ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。


Electrical and handling symbols

Electrical symbols

These can appear in instrument user manuals and on the instrument’s front or rear panels.

Electrical power on

Electrical power off

Standby

Direct current

Alternating current

Protective conductor terminal

Frame, or chassis, terminal

Fuse

Recycle symbol: Do not dispose in municipal waste.

Electrical and handling symbols A-7

Handling symbols

These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments.

Keep upright!

Keep dry!

Fragile!

Use no hooks!


Index

AAPCI corona pin 1-6API vASAP

compatibility with instruments 1-2operating principles 1-3

audience and purpose v

Bbackground ions, reducing 2-3biohazard warning A-3

Ccalibration 2-2caution symbol A-3chemical hazard warning A-4

Ddata, acquiring 2-4desolvation gas

flow rate 2-3, 2-4temperature 2-3, 2-10

EEC Authorized Representative vielectrical symbols A-7equipment guidelines v, A-5ESCi corona pin 1-6

Ggas leakage hazard iv

Hhandling symbols A-8hazards

gas leakage ivhigh temperature ivpuncture iv

hexachlorobenzene spectra 2-9high temperature hazard iv

Iintended use vISM classification vi

LLCT Premier XE 2-6liquid 2-3loading 2-3LockSpray interface 1-6

Mmethod parameters

3100 detector 2-18LCT Premier XE 2-12Q-Tof Premier 2-13SQ Detector 2-18SQ Detector 2 2-17SYNAPT 2-15SYNAPT G2 HDMS 2-19SYNAPT G2 MS 2-19TQ detector 2-18Xevo G2 QTof MS 2-21Xevo G2 Tof 2-21Xevo QTof MS 2-17Xevo TQ MS 2-17Xevo TQD 2-17Xevo TQ-S 2-21

Nnon-polar compounds, analyzing 2-8

Pparameters, starting 2-4puncture hazard ivpurpose and audience v

Index-1

Ssafety advisories iv, A-1samples 2-3

analyzing 2-4solution 2-4

solid 2-3source baking 2-3starting parameters 2-4symbols

caution A-3electrical A-7handling A-8warning A-2

Tthermal fragmentation 2-10

Uusing modifiers 2-11

Wwarning symbols A-2, A-5

Index-2

atmospheric solids analysis probe - waters corporation · 2012-01-10 · inserting the inner probe...

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