JOURNAL OF BACTERIOLOGYVOLUME 101 * NUMBER 3 * MARCH 1970

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)Unziversity of Illinois, UrbanaL. S. BARON, Editor (1970)

Wailer Reed Army Institute of ResearchWashington, D.C.

R. D. DeMOSS, Editor (1970)University oJ Illinois, Urbana

ALLEN G. MARR, Editor (1973)University oj California, Davis

Howard I. Adler (1970)R. L. Anderson (1972)W. Lane Biirksdale (1972)Robert W. Bernlohr (1970)S. Gaylen Bradley (1970)Arnold F. Brodie (1970)Allan Campbe!l (1972)V. P. Cirillo (1970)Royston C. Clowes (1972)Wa'nllacc A. Clyde, Jr. (1970)V. W. Cochrane (1972)S. F. Conti (1972)Roy Curtiss HI (1972)A. L. Demain (1972)John A. DeMoss (1972)Martin Dworkin (1970)Ellis Englesberg (1972)Stanley Falkow (1972)Leonard Frank (1970)Martin Freundlich (1970)Herman Friedman (1972)A. T. Ganesan (1972)Howard Goldfine (1972)Joseph S. Gots (1972)

Harlyn 0. Halvorson (1972)F. M. Harold (1970)Leonard Hayflick (1972)George Hegeman (1971)Charles E. Helmstetter (1972)D. S. Hoare (1971)Joseph T. Holden (1972John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1970)John H. Law (1971)Edward R. Leadbetter (1970)Hillel S. Levinson (1972)H. C. Lichstein (1970)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)M. Nomura (1971)Richard P. Novick (1970)Kenneth Paigen (1972)

Harry D. Peck (1970)H. J. Phaff (1972)Jack Preiss (1971)H. V. Rickenberg (1970)M. R. J. Salton (1972)Moselio Schaechter (1970)David Schlessinger (1971)W. R. Sistrom (1972)Paul F. Smith (1970)Roger Y. Stanier (1970)M. P. Starr (1970)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1970)Arthur Weissbach (1971)N. E. Welker (1970)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1970)Barbara E. Wright (1971)Frank E. Young (1970)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOE. M. FOSTER, President (1969-1970)

DONALD E. SHAY, SecretaryThe Journal of Bacteriology, a publication of the American

Society for Microbiology, 4715 Cordell Ave., Bethesda, Md.20014, is devoted to the advancement and dissemination of funda-mental knowledge concerning bacteria and other microorganisms.The Journal is published monthly, and the twelve numbers aredivided into four volumes per year. The subscription price is$40.00 (Postal Union Countries, $42; other foreign, $43) peryear. Single copies are $4.00 (foreign, $4.25). Members of theAmerican Society for Microbiology may receive the Journal aspart of their dues Correspondence relating to reprints, defectivecopies, availability of back issues, lost or late proofs, disposi-tion of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Office, 4715 CordellAve., Bethesda, Md. 20014. Correspondence from ASM mem-

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bers relating to membership dues, member subscriptions,changes of address, incorrect address, incorrect journals, etc.,should be directed to the American Society for Microbiology,1913 I St., N.W., Washington, D.C. 20006. Nonmembers ofthe Society should write to The Williams & Wilkins Co., 428 E.Preston St., Baltimore, Md. 21202, regarding institution of non-member subscriptions and nonreceipt of journals. Publishedmonthly by the ASM at 428 E. Preston St., Baltimore, Md.21202.

Second class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices.Made in the United States of America.Copyright © 1970, American Society for Microbiology.All Rights Reserved

Author IndexAdams, Elijah, 948Alexander, A. D., 827Angelo, Noreen, 717

Barlati, Sergio, 925Beattie, Kenneth L., 808Becker, George E., 821Benson, Charles E., 872Bonamy, Celine, 1027Borek, Ernest, 705Brendle, J. J., 827Brody, Stuart, 802Burman, Lars G., 659Burnham, Jeffrey C., 997Burris, R. H., 794Bussey, Howard, 1081

Cabrera-Juairez, Emiliano, 681Carbonell, Luis M., 675Chai, Ning-Chun, 1005Cole, Roger M., 1046Conti, S. F., 989, 997, 1038Costerton, J. W., 1014

Davies, Martin, 836De Ley, J., 738Demohn, V., 1073DeMoss, R. D., 813Denny, C. F., 989De Voe, I. W., 1014Dickinson, Ellen S., 1090Diedrich, D. L., 989Drews, G., 669Dugan, Patrick R., 873, 982Dunnick, June K., 892

Elbein, Alan D., 777Elkan, Gerald H., 698Epstein, Wolfgang, 836Eriksson-Grennberg, Kerstin G., 659

Freese, Elisabeth Bautz, 1046Freese, Ernst, 1046Friedman, John, 921Fukui, Sakuzo, 685, 692

Goldman, Dexter S., 781Goto, Nobuichi, 856Gottlieb, David, 755Griffiths, S. K., 813Gryder, Rosa M., 948

Haapala, D. K., 827Hamilton, Pat B., 698

Hashimoto, T., 989, 997, 1038Hayano, Koichi, 692Hey-Ferguson, Ann, 777Higgins, Thomas E., 885Hirai, K., 1086Hirshfield, I. N., 725Hoar, David I., 916

Inouye, Masayori, 770Ishikawa, Yoshinori, 881Iwashima, Akio, 1076

Jamaluddin, M., 786Jansen, H. M., 1083Jensen, Roy A., 763Johnson, Marvin J., 885Jorstad, Caroline M., 731

Kanetsuna, Fuminori, 675Kato, Masahiko, 709Keele, Bernard B. Jr., 698Kindel, Paul K., 910Klofat, Walther, 1046Kosower, Edward M., 1092Kosower, Nechama S., 1092Kusaka, Takashi, 781

Laporte, J., 1027Lark, K. G., 1005Leifer, Z., 725Leighton, T. J., 931Lieberman, Michael M., 959, 965Lindstedt, G., 1094Lindstedt, S., 1094Love, Samuel H., 872

Maas, W. K., 725MacLeod, Robert A., 1014MacMillan, Carol B., 973, 982Mahadevan, P. R., 941Mahadkar, U. R., 941Mandel, M., 844Markovitz, Alvin, 959, 965Melville, Donald B., 881Midtvedt, T., 1094Miyairi, Sachio, 685Morris, David R., 731

Nakaya, Rintaro, 856Neal, Donna L., 910Newsom, S. W. B., 1079Nordstrom, Kurt, 659Nose, Yoshitsugu, 1076

Obrig, T. G., 755Oelze, J., 669O'Leary, William M., 892Omenn, Gilbert S., 921Ornston, L. N., 1088Ornston, M. K., 1088Otsuki, Koichi, 1063

Pardee, Arthur B., 770, 901Pfister, Robert M., 973, 982Polsinelli, Mario, 851Ponce-De Leon, Manuel, 681

Rao, P. V. Subba, 786Reinhold, Vernon N., 881Remy, Charles N., 872Renner, E. D., 821Richmond, M. H., 1079Rogul, M., 827Rosenberg, Eugene, 1092Rosenfeld, H. J., 725

Samsonoff, William A., 1038Schroeder, J., 669Setlow, Jane K., 808Shaparis, Anna, 959Sharma, Opendra K., 705Sinohara, Hyogo, 1070Smith, Helene S., 901Somma, Sergio, 851Stenmark, Sherry L., 763Stock, J. J., 931Sundaram, T. K., 1090Suzuki, Kenji, 856Sykes, R. B., 1079Szulmajster, J., 1027

Terawaki, Yoshiro, 856Tevethia, M. J., 844Thompson, J., 1014Tofft, M., 1094Tomasz, Alexander, 860

Vaidyanathan, C. S., 786Vandecasteele, Jean-Paul, 794van Houte, J., 1083

Wang, Lynn, 781Wax, Richard, 1092Weisblum, B., 1073Wittenberger, Charles L., 717

Yanagawa, Ryo, 1063, 1086Yoshida, Yoko, 856

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AUTHOR INDEX

VOLUME 101

Abram, Dinah, 250Adams, Elijah, 948Alexander, A. D., 827Anderson, Robert S., 568Angelo, Noreen, 717Armstrong, Donald, 346

Baarda, J. R., 152Barile, Michael F., 346Barlati, Sergio, 166, 330, 350,

355, 925Bayer, M. E., 304Beaman, T. Cabrera, 196Beattie, Kenneth L., 808Beck, Reta S., 408Becker, George E., 821Benson, Charles E., 872Berg, Alan P., 438Bergendahl, Janet, 333Bernheim, Naomi J., 384Bicknell, J. N., 505Boatman, Edwin S., 262Bonamy, Celine, 1027Boman, Hans G., 218Borek, Ernest, 705Brendle, J. J., 827Brody, Stuart, 802Brock, Thomas D., 541Brown, Albert T., 456Burden, Linda,Burman, Lars G., 659Bumham, Jeffrey C., 997Burris, R. H., 794Bussey, Howard, 1081Byers, B. R., 181

Cabrera-Juarez, Emiliano, 681Carbonell, L. M., 636, 675Carmody, Janice M., 525Carrow, J. A., 141Carski, Theodore R., 346Catena, Anthony, 476Chai, Ning-Chun, 1005Ciferri, Orio, 166, 350Cole, Roger M., 1046Conti, S. F., 989, 997, 1038Costerton, J. W., 1014Costilow, Ralph N., 77

Darnall, Dennis W., 65Daron, Harlow H., 145Davies, Martin, 836Davis, W. B., 181DeCicco, B. T., 339De Ley, J., 738Decker, G. L., 618Del Giudice, Richard A., 346Delafield, F. P., 551Delwiche, E. A., 138, 141

Demohn, V., 1073DeMoss, R. D., 476, 813Denny, C. F., 989De Voe, I. W., 1014Dickinson, Ellen S., 1090Diedrich, D. L., 989Dobrogosz, Walter J., 384Dodson, J. L., Jr. 333Domnas, A., 652Donaldson, David M., 314Douglas, Howard C., 505Downer, D. N., 181Drews, G., 669Dugan, Patrick R., 973, 982Dunnick, June K., 892Dworkin, Martin, 325, 531Dyer, John K., 77

Eanes, E. D., 464Eberhart, Bruce M., 408Elbein, Alan D., 777Elkan, Gerald H., 698Elmer, Gary W., 592, 595Epstein, Wolfgang, 836Eriksson-Grennberg, Kerstin G.,659

Evenchik, Z., 418

Field, Marvin F., 628Fisher, Robert J., 292Fister, George, 188Fjellstedt, Thorsten A., 108Fowler, Audree V., 438Freese, Elisabeth Bautz, 1046Freese, Ernst, 1046Freeze, Hudson, 541Friedman, John, 921Fukui, Sakuzo, 685, 692

Garrison, Robert G., 628Gerhardt, Philipp, 196Gherna, R. L., 464Gil, F., 636Gillespie, David, 584Gilvarg, Charles, 323Goldman, Dexter S., 781Goto, Nobuichi, 856Gottlieb, David, 755Grant, Neil G., 652Greenawalt, J. W., 618Griffiths, S. K., 813Gryder, Rosa M., 948Guild, W. R., 361

Haapala, D. K., 827Hageage, G. J., Jr., 464Hamilton, Pat B., 698Hanson, R. S., 24

i

Harold, F. M., 152Hartman, Philip E., 490, 649Harvey, R. J., 574Hashimoto, T., 989, 997, 1038Hayano, Koichi, 692Held, William A., 202, 209Hendriks, T., 423Herriott, Roger M., 513, 517, 525Hey-Ferguson, Ann, 777Higgins, Michael L., 643Higgins, Thomas E., 885Hildebrand, D. C., 9Hirai, K., 1086Hirshfield, I. N., 725Hoar, David I., 916Hyatt, Mildred T., 58

Igambi, Levi, 333Imsande, John, 173Inouye, Masayori, 770Ishikawa, Yoshinori,881Issahary, Gabriela, 418Iwashima, Akio, 1076

Jamaluddin, M., 786Jansen, H. M., 1083Jensen, Roy A., 763Johnson, John L., 568Johnson, Marvin J., 885Jordan, B., 657Jorstad, Caroline M., 731

Kanazir, Dusan T., 649Kanetsuna, F., 636, 675Kato, Masahiko, 709Keele, Bernard B., Jr., 698Keith, Alex D., 160Kempner, E. S., 561Kenny, George E., 262Keynan, A., 418Khuller, G. K., 654Kindel, Paul K., 910Klofat, Walther, 1046Knudson, Dennis L., 609Koffler, Henry, 250Kornberg, Arthur, 327Kosower, Edward M., 1092Kosower, Nechama S., 1092Kusaka, Takashi, 781

Lacks, Sanford, 373Lane, James W., 628Lang, Dennis R., 483Laporte, J., 1027Lark, K. G., 1005Lechowich, Richard V., 118Lederberg, S., 398Leifer, Z., 725Leighton, T. J., 931

AUTHOR INDEX

Levinson, Hillel S., 58Lewis, Harold L., 65Lieberman, Michael M., 959, 965Lindqvist, R. Christer, 232Lindstedt, G., 1094Lindstedt, S., 1094Lindstrom, E. Borje, 218Love, Samuel H., 872Lovett, Paul S., 603Lundgren, D. G., 483

Maas, W. K., 725McElhaney, Ronald N., 72MacKechnie, Ian, 24MacLeod, Robert A., 1014MacLeod, Roderick, 609MacMillan, Carol B., 973, 982Mahadevan, P. R., 941Mahadkar, U. R., 941Majerfeld, Irene, 350, 355Mallavia, Louis P., 127Mandel, M., 333, 844Manire, G. P., 278Marcus, Leon, 286, 292Markovitz, Alvin, 959, 965Marmur, Julius, 449Marquis, Robert E., 92Marr, A. G., 561Matheson, Audria, 314Matsumoto, A., 278Matz, L. L., 196Melville, Donald B., 881Meyer, Eleanor Y., 513, 517Miao, Raymond, 361Michaud, R. N., 138, 141Midtvedt, T., 1094Mitchen, J. R., 250Miyairi, Sachio, 685Mizuno, D., 102Modan, Michaela, 513Morris, David R., 731Muhammed, Amir, 444

Nakaya, Rintaro, 856Nanninga, N., 297Neal, Donna L., 910Newsom, S. W. B., 1079Nickerson, Walter J., 592, 595Nixon, Ralph, 584Nordstrom, Kurt, 232, 659Nose, K., 102Nose, Yoshitsugu, 1076

Obrig, T. G., 755Oelze, J., 669Oeschger, Nicole S., 490Ogur, Maurice, 108

O'Leary, William M., 892Omenn, Gilbert S., 921Ono, M., 102Oppenheim, Joel, 286, 292Ordal, Erling J., 568Ornston, L. N., 1088Ornston, M. K., 1088Otsuki, Koichi, 1063Ou, Li-Tse, 92

Pardee, Arthur B., 770, 901Patterson, David, 584Pavlasova, E., 152Pfister, Robert M., 973, 982Polsinelli, Mario, 851Ponce-De Leon, Manuel, 681Previc, Edward, 188

Raj, H. D., 240Ramaley, Robert F., 1Ramsey, W. Scott, 531Rao, P. V., Subba, 786Ravin, Arnold W., 38Razin, Shmuel, 346Reichenbach, Hans, 325Reinhold, Vernon N., 881Remsen, C. C., 304Remy, Charles N., 872Renner, E. D., 821Resnick, Michael R., 160Reyngoud, D. J., 423Richmond, M. H., 1079Rittenberg, S. C., 551Rizza, Victor, 84Rogul, M., 827Rosenberg, Eugene, 1092Rosenfeld, H. J., 725Roth, Robert, 53

Samsonoff, William A., 1038Sands, D. C., 9Savic, Dragutin, 649Scheltgen, E., 333Schroeder, J., 669Schroth, M. N., 9Setlow, Jane K., 444, 808Shaparis, Anna, 959Sharma, Opendra K., 705Shively, J. M., 618Shockman, Gerald D., 643Siersma, P. W., 398Silverman, G. J., 429Sinclair, Peter R., 365Sinohara, Hyogo, 1070Sinskey, T. J., 429Smith, Helene S., 901

Smith, Oliver H., 202, 209Snellen, James E., 240Somerville, H. J., 551Somma, Sergio, 851Steele, Barbara B., 218Stenmark, Sherry L., 763Stewart, Charles R., 449Stock, J. J., 931Stukus, Philip E., 339Strauss, Norman, 35Subrahmanyam, D. 654Sundaram, T. K., 1090Suzuki, Kenji, 856Sykes, R. B., 1079Szulmajster, J., 1027

Takahashi, Elaine A., 38Terawaki, Yoshiro, 856Tevethia, M. J., 844Thompson, J., 1014Tofft, M., 1094Tomasz, Alexander, 860Tourtellotte, Mark E., 72Tucker, Anne N., 84Tully, Joseph G., 346

Vaidyanathan, C. S., 786Vandecasteele, Jean-Paul, 794Van den Ende, H., 423van Houte, J., 1083Vary, James C., 327Vatter, A. E., 250Vogt, Marcella, 513, 517Voss, J. G., 392

Wagner, Conrad, 456Wang, Lynn, 781Wax, Richard, 1092Webster, Francis H. 118Weinberger, Steven, 323Weinstein, Mark 584Weisblum, B., 1073Weiss, Emilio, 127, 133White, David C., 84, 365Wiechmann, A. H. C. A., 423Wildermuth, H., 318Wilson, P. W., 292Winkler, Herbert H., 470Wisnieski, Bernadine J., 160Wittenberger, Charles L., 717

Yanagawa, R., 1063, 1086Yoshida, Yoko, 856Young, Frank E., 603

Zabin, Irving, 438

* @

J. BACTERIOL.

SUBJECT INDEX

VOLUME 101

Acetate-utilization in E. coli and A. cloacae, 885Acetyl blocking groups-in diaminopimelic acid biosynthesis, 323N-acetylglucosamine-and nongrowth of E. coli mutants, 384Acid mine water (pH 2)-heterotrophic growth, 973, 982Acid streamers-in coal mine drainage, 973, 982Acidic coal mine drainage-characterization, 973, 982Acridine half-mustards (ICR191)-and mutations in the histidine operon of Salmonella,490

Actinobacillus mallei-similarity of DNA to that of P. multivoransand P. pseudomallei, 825

Actinomycin-and amino acid starvation in B. subtilis, 925Actinomycin D-effect of transfer of RNA, 1005Actinoplanaceae-cytochrome composition, 652Activation-of B. megaterium spores, 58S-adenosylmethionine hydrolase-bacteriophage-induced, 398Aerobacter aerogenes-metabolism of D-apiose reductase from, 910Aerobacter cloacae-pathways of anaerobic acetate utilization, 885Aging-and protein synthesis in R. solani, 755Agrobacterium tumefaciens-active transport of glucose-l-phosphate, 685-a-3-ketoglucosidase, 692Air-grown Azotobacter-membrane network, 286Aldolase-thermostable, from T. aquaticus, 541Allostery-comparative, of DAHP synthetase, 763Alpha-3-ketoglucosidase-of A. tumefaciens, 692Amino acid starvation-in B. subtilis, 925Amino sugar sensitivity-in E. coli, 384Ampicillin-resistant mutant-of E. coli, 659Anaerobic acetate utilization-in A. cloacae, 885-in E. coli, 885Anthranilate synthetase-of B. alvei, 476Antibiotic resistance-and bacterial lipids, 892

iii

D-Apiose reductase-metabolism from A. aerogenes, 910Aromatic biosynthesis-in E. coli, 202Aspergillus niger-fatty acid toxicity and methyl ketone production, 65-metabolism of mandelic acid, 717Aspergillus oryzae-induction of enzymes in dormant spores, 1070Autoradiography-in study of competence and DNA incorporation

in B. subtilis, 851Autotrophic growth-regulation by organic substrates, 339Azotobacter vinelandii-correlation of ultrastructure with nitrogen source

for growth, 286-properties of a soluble nitrogenase, 292

Bacillus alvei-anthranilate synthetase, 476-L-serine dehydratase and tryptophanase from, 813Bacillus cereus-chemical compostion of exosporium from spores,

196-kinetics of penicillinase induction, 173-liquid composition, 483Bacillus cereus spores-low-pH activation, 418Bacillus cereus T-microcycle sporogenesis, 24Bacillus megaterium-sporulating; dihydrodipicolinic acid synthetase, 118Bacillus megaterium spores-effects of temperature on activation, germination,and outgrowth, 58

Bacillus pumilus-differentiation within the bacterial flagellum and

isolation of the proximal hook, 250-genetic analysis by PBSl-mediated transduction, 603Bacillus sp.-fatty acid composition of lipid extracts, 145Bacillus subtilis-amino acid starvation, 925-competence and DNA incorporation, 851-EDTA requirement for transformation, 844-effect of j3-Jysis on cell walls and protoplasts, 314-factors responsible for tryptophanless death, 355-glucosamine mutants, 1046-incorporation into DNA, 330-incorporation of 5-methyl- and 5-hydroxy-trypto-phan into protein, 166

-lytic activity and DNA uptake, 449-polyribosomes, 925-repression of phenolic acid-synthesizing enzymesand its relation to iron uptake, 181

-temperature-sensitive sporulation mutant, 1027-tryptophanless death, 350, 355

SUBJECT INDEX

Bacillus subtilis 168-replacement sporulation, 1Bacillus thuringiensis-urea-mercaptoethanol-soluble protein from spores,

551Bacteria-indigenous to acid mine water, 973, 982-merodiploid states, 38-slime-producing, in acid mine water, 982Bacterial deoxyribonucleic acid-correlation of melting temperature and cesium

chloride buoyant density, 333Bacterial division-requirement for polyamines, 770Bacterial flagellum-differentiation, 250Bacterial germination-biochemical studies, 327Bacterial lipids-and antibiotic resistance, 892Bacterial sporulation-biochemical studies, 327Bacteriochlorophyll synthesis-and thylakoid formation in R. rubrum, 669Bacteriophage-induced S-adenosylmethionine hy-

drolase-in vivo suppression of coding, 398Bacteriophage RP-28-and transduction in C. renale, N-7Bacteriophage SPP1-incorporation of uridine into DNA, 330Bacteroides melaninogenicus-lipids, 84Bdellovibrio bacteriovorus-facultatively parasitic strain, 989, 997Beta-galactosidase-isolation, 438Beta-glucosidases-localization in N. crassa, 408Beta-lysin-effect on cell walls and protoplasts of B. subtilis, 314Biosynthesis-of diaminopimelic acid, 323-of purine, inhibition, 872-of tryptophan in E. coli, 202, 209Biosynthesis of lysine-and supersuppressor genes, 108Blakeslea trispora-and M. mucedo, hormonal interactions, 423Blastomyces dermatitidis-yeast-to-mold morphogenesis, 628Blocking groups-in diaminopimelic acid biosynthesis, 323Branching-the role of cell wall-bound enzymes, 941Buoyant density-association with melting point and chemical base

composition of DNA, 738-of DNA from temperature-sensitive R factor, 856y-Butyrobetaine hydroxylase-activity in Pseudomonas sp., 1094

Calcium-effect of deficiency on morphogenesis and fine

structure of L. mucor, 240

Canine mycoplasmas-new classification, 346Capsular polysaccharide synthesis-in E. coli K-12, 965Carbenicillin-,-lactamase active against in P. aeruginosa, 1079Carbohydrate accumulation-during sporulation of yeast, 53Carbon dioxide-and growth of M. rouxii, 595Carnitine dehydrogenase-activity in Pseudomonas sp., 1094Catabolic activities-of N. meningitis, 127, 133Catabolism-of gluconate in R. japonicum, 698Cations (monovalent)-induction of myxospore formation in S. aurantiaca,

325Cell envelope-of E. coli B after freeze-etching, 297Cell wall-of N. crassa, 941Cell wall tonus-of cocci, 92Cellular metabolism-in genetic transformation of pneumococci, 860Cesium chloride buoyant density-correlation with melting point of bacterial DNA,

333Chemical composition-association with melting point and buoyant density

of DNA, 738Chitin-and glucan in P. brasiliensis, 636Chlamydia psittaci-effect of penicillin on moprhology, 278Chloroform-methanol-extraction of lipids of B. melaninogenicus, 84Chromatium species-sulfur globules, 464Chromosomally mediated penicillinase-in E. coli, 218Clostridial spore-germination, 1038Clostridium bifermentans-germination of appendage-bearing spores, 1038Clostridium pasteurianum-constituents of the nitrogenase complex, 794Clostridium sticklandii-anaerobic oxidation of ornithine, 77Clostridium-and 2,4-diaminovaleric acid, 77Coal mine drainage,-indigenous bacteria, 973, 982Cocci-gram-positive; cell wall tonus, 92Coding-in vivo suppression of associated with phage-

induced S-adenosylmethionine hydrolase, 398Colicin E2-actions, 102-stabilizing effect on spheroplast membrane, 102Colony growth-of Mycoplasma, 609

iV J. BACTERIOL.

SUBJECT INDEX

Competence-in B. subtilis, 851-in H. influenzae, 517Corynebacterium acnes-differentiation of two groups, 392Corynebacterium diphtheriae-action of a toxic glycolipid on mitochondrial

structure and function, 709Corynebacterium nephridii-production of nitric oxide and nitrous oxide, 821Corynebacterium renale-generalized transduction, 1086-properties of pili, 1063Cytochrome composition-of Actinoplanaceae, 652

Defined medium-for competence development in H. influenzae, 517-for growth of H. influenzae, 513Denitrification-by C. nephridii, 8213-Deoxy-D-arabino-heptulosonate-7-phosphate syn-

thetase-comparative allostery, 763Deoxyribonucleases-mutants of D. pneumoniae lacking, 373Deoxyribonucleic acid-incorporation in B. subtilis, 851-labeling in H. influenzae, 525-melting point, buoyant density, and chemical base

composition, 738-of B. subtilis and SPP1 bacteriophage; incorporation

of uridine, 330-of temperature-sensitive R factor, 856-single and double stranded; acceptance by com-petent D. pneumoniae, 361

-single stranded, transformation of B. subtilis, 844-similarities among pseudomonads, 825-ultraviolet-irradiated transforming, 808Deoxyribonucleic acid (bacterial)-correlation of melting temperature and cesium

chloride buoyant density, 333Deoxyribonucleic acid (transforming)-decrease in integration, UV induced, 444-early energy-dependent step in entry, 35-from H. influenzae, 681Deoxyribonucleic acid uptake-in B. subtilis, 449Derepression-of GDP-mannose pyrophosphorylase, 965Diamide-effect on growth of E. coli, 1092Diaminopimelic acid biosynthesis-succinyl and acetyl blocking groups, 3232, 4-Diaminovaleric acid-and anaerobic oxidation of ornithine, 77S-Diazouracil-2-14C-incorporation into RNA of E. coli, 188Dictyostelium discoideum-inhibitor of tRNA methylases in, 705Differentiation-within the bacterial flagellum, 250Dihydrodipicolinic acid synthetase-from sporulating B. megaterium, 118-purification and characterization, 118

Diplococcus pneumoniae-acceptance of single- and double-stranded DNA

361-mutants lacking deoxyribonucleases, 373Division-bacterial, requirement for polyamines, T-16-in E. coli, 901Division cycle-of E. gracilis, 561Double-bond requirement-in a fatty acid desaturase mutant of S. cerevisiae,

160Duplication cycle-of E. coli, 901

Electromechanical interactions-in cell walls of gram-positive cocci, 92Electron microscopy-of colony growth of Mycoplasma, 609-of effect of penicillin on morphology of C. psittaci,

278Electron transport system-of H. parainfluenzae, 365Elongation

of fatty acids in M. tuberculosis, 781End-product inhibitor-insensitive mutants-in yeast, 1081Endoparasitic mutant

of B. bacteriovorus, 989, 997Energy-dependent step-in the entry of transforming DNA, 35Enzyme activities-in extracts of cells grown without substrate, 1094Enzyme defects-of glucosamine mutants of B. subtilis,. 1046Enzymes-cell wall-bound, 941-in growth and morphology of N. crassa, 941-involved in conversion of tryptophan to NAD, 456-phenolic acid-synthesizing, 181Escherichia coli-accumulation of protein required for division, 901-amino sugar sensitivity, 384-aromatic biosynthesis, 202-chromosomal location of a gene defining nicotin-amide deamidase, 1090

-effect of diamine on growth, 1092-effect of glycine on thiazole biosynthesis, 1076-effect of 6-methylpurine on purine biosynthesis and

interconversion, 872-Hexose-6-phosphate transport system, 470-incorporation of 5-diazouracil-2-14C into RNA, 188-injury incurred by freeze-drying, 429-mutant blocked in the synthesis of putrescine, 725-pathways of anaerobic acetate utilization, 885-putrescine-deficient mutants, 731-regulation of ribosomal protein synthesis, 574-regulation of the tryptophan operon, 202, 209-resistance to penicillins, 218, 232, 659-ribosome-membrane complex, 584Escherichia coli-structure after freeze-etching, 304Escherichia coli-uridine diphosphate-glucose dehydrogenase in

wild-type, K-12, 959

VOL. 101 , 1970 V

SUBJECT INDEX

Escherichia coli B-ultrastructure of the cell envelope after freeze-

etching, 297Escherichia coli K-12-capsular polysaccharide synthesis, 965-glycolate uptake by mutant species, 1088-mutants dependent upon potassium, 836Escherichia coli W3110-treatment with col E2, 102Ethylenediaminetetraacetic acid-in single-strand DNA transformation, 844Euglena gracilis-growth in volume during division cycle, 561Exosporium-from spores of B. cereus, 196

Fatty acid desaturase mutant-of S. cerevisiae, 160Fatty acid elongation-in M. tuberculosis, 781Fatty acid synthesis-and thylakoid formation in R. rubrum, 669Fatty acid toxicity-and methyl ketone production in A. niger, 65Fatty acids-of a Bacillus sp., 145Fertility regulation-in F-like resistance transfer factors, 916Filamentous growth-of M. rouxii under nitrogen, 592Fine structure-of L. mucor, 240Frameshift mutations-in the histidine operon of Salmonella, 490Freeze-drying-injury to E. coli, 429Freeze-etching-of E. coli, 304-of E. coli cell envelope, 297-to determine surface structure of streptomycete

spores, 318Fructose-I, 6-diphosphate activated-lactate dehydrogenase from S. faecalis, 717Fumarate-effect on electron transport system of H. parain-fluenzae, 365

Fungal sporulation-biochemical changes, 931Fungal sporulation and spore germination-biochemical changes, 931Fungi-morphogenesis, 628

,j-Galactosidase-isolation, 438Gene-specifying nicotinamide deamidase in E. coli, 1090Gene-protein relationship-in the lac operon, 438Genes (supersuppressor)-and lysine biosynthesis, 108Genetic transformation-of pneumococci, 860Germination-of a clostridial spore, 1038

-of B. megaterium spores, 58-of M. xanthus microcysts, 531-temperature-sensitive mutants for initiation, 327Glucan-and chitin in P. brasiliensis, 636Glucans-of P. brasiliensis, 675Gluconate-catabolism in R. japonicum, 698Glucosamine mutants-of B. subtilis, 1046Glucose-i-phosphate-active transport in A. tumefaciens, 685,-Glucosidases-localization in N. crassa, 408Glutamate-utilization in N. meningitis, 127Glutathione-oxidation in E. coli by diamine, 1092Glycine-effect on thiazole biosynthesis in E. coli, 1076Glycogen-role in survival of S. mitis, 1083Glycolate-uptake by mutants of E. coil K-12, 1088Glycolipid (toxic)-of C. diphtheriae, action on mitochondrial structureand function, 709

Glycolysis-multiple impairment in V. alcalescens, 138Gram-negative bacterium-cells, mureinoplasts, and protoplasts, 1014Gram-positive cocci

electromechanical interaction in cell walls, 92Growth-filamentous, of, M. rouxii under nitrogen and underC02, 592, 595-in volume of E. gracilis during division cycle, 561-of H. influenzae, 513, 517Growth and sporulation-of B. cereus, 483Guanosine diphosphate-mannose pyrophosphorylase-derepression, 959

Haemophilus influenzae-defined medium for growth, 513-defined nongrowth media for stage II development

of competence, 517-labeling of DNA, 525-native and denatured transforming DNA, 681-nongrowth media, 517-repair of UV-irradiated DNA, 808-thymine and thymidine uptake, 525-UV-induced decrease in integration, 444Haemophilus parainfluenzae-electron transport system, 365Hercynine-conversion from histidine by N. crassa, 881Heterotrophic growth-in acid mine water, 973, 982Hexokinase deficiency-in V. alcalescens, 138Hexose-6-phosphate transport system-of E. coli, 470

V1 J. BACTERIOL.

SUBJECT INDEX

Histidine-conversion to hercynine by N. crassa, 881Histoplasma capsulatum-yeast-to-mold morphogenesis, 628Hormonal interactions-in M. mucedo and B. trispora, 423Hormones-in Mucor and Blakeslea, 423Host-dependent growth-of B. bacteriovorus, 989, 997Host-independent growth-of B. bacteriovorus, 989, 997Hydrogen and sodium ions-extrusion by S. faecalis, 152Hydrogenomonas-metabolism, 3395-Hydroxy-tryptophan-incorporation into protein of B. subtilis, 166Hydroxyproline-transport in P. putida, 948

Inducible hydroxyproline transport-of P. putida, 948Induction-of enzymes in dormant spores of A. oryzae, 1070-of penicillinase in B. cereus 569, 173-of the hexose-6-phosphate transport system, 470Injury-to E. coli by freeze-drying, 429Ions-of sodium and hydrogen by S. faecalis, 152Iron uptake-in B. subtilis, and repression of phenolic-acid

synthesizing enzymes, 181Isomerase (D-mannose)-from M. amegmatis, purification, 777

a-3-ketoglucosidaseof A. tumefaciens, 692

Kineticsof anthranilate synthetase of B. alvei, 476

j3-Lactamase-active against carbenicillin in P. aeruginosa, 1079Lactate dehydrogenase-from S. faecalis, 717-fructose-l, 6-diphosphate activated, from S. faecalis,717

Lactobacillus acidophilus-effect of actinomycin D on RNA transfer, 1005

14C leucine-effect of thiostrepton on incorporation, 1073Leucothrix mucor

-morphogenesis and fine structure, 240Lipid composition-and antibiotic resistance, 892Lipids-bacterial, and antibiotic resistance, 892-in B. cereus, 483-of B. melaninogenicus, 84Lysine biosynthesis-and supersuppressor genes, 108Lysozyme-mediation of the ribosome-membrane complex ofE. coli, 584

Lytic activity-in cells of B. subtilis after DNA uptake, 449

Macroconidial germination-inhibition by phenyl methyl sulfonyl fluoride, 931-phenyl methyl sulfonyl fluoride inhibition in M.gypseum, 931

Macromolecular synthesis-during microcycle sporogenesis of B. cereus T, 24Mandelic acid metabolism-by A. niger, 786D-Mannose isomerase-from M. amegmatis, purification, 777Medium-defined, for growth of H. influenzae, 513Melting point-association with buoyant density and chemical

base composition of DNA, 738Melting temperature-correlation with cesium chloride buoyant density

of bacterial DNA, 333Membrane network-in air-grown Azotobacter, 286Membrane polar lipids-of M. laidiawii B, 72Merodiploid ribosomal loci-by transformation and mutation in pneumococcus,

38Merodiploid states-in bacteria, 38Messenger ribonucleic acid-and germination of M. xanthus microcysts, 531Metabolic inhibitors-effect on genetic transformation in pneumococci,860

Metabolic turnover-of polar lipids of M. laidiawii B, 72Metabolism-of D-apiose reductase from A. aerogenes, 910-of Hydrogenomonas, 339

of mandelic acid by A. niger, 786-of polar lipids, 72Methyl ketone production-and fatty acid toxicity in A. niger, 655-Methyl-tryptophan-incorporation into protein of B. subtilis, 1663-Methylanthranilic acid derepression-of the tryptophan operon in E. coli, 209Methylases (transfer ribonucleic acid)-inhibitor of in D. discoideum, T-86-Methylpurine-inhibitory effect, 872Microcycle sporogenesis-of B. cereus T, 24Microcysts-of M. xanthus, 531Microsporum gypseum-inhibition of macroconidial germination, 931-phenyl methyl sulfonyl fluoride inhibition of

macroconidial germination, 931Mitochondrial structure and function-effect of a toxic glycolipid of C. diphtheriae, 709Molecular classification-by comparative allostery of DAHP, 763

* a

VOL. 101, 1970

SUBJECT INDEX

Monovalent cations-induction of myxospore formation in S. aurantiaca,

325Moraxella species-nucleic acid homologies, 568Morphogenesis-of fungi, 628-of L. mucor, 240Morphology-of C. psittaci, effect of penicillin, 278-of M. felis, 262-of N. crassa, T-41-of strains of N. crassa, T-22Mucor mucedo-and B. trispora, hormonal interactions, 423Mucor rouxii-growth under nitrogen and under Co2, 592, 595Mureinoplasts-of a gram-negative bacterium, 1014Mutant-of E. coli blocked in the synthesis of putrescine,725

-sporulation in B. subtilis, 1027Mutant-fatty acid desaturase, of S. cerevisiae, 160Mutants-end-product inhibitor-insensitive in yeast, 1081-of D. pneumoniae that lack deoxyribonucleases, 373-of E. coli, ampicillin resistant, 659-of E. coli, potassium dependent, 836-of E. coli, putrescine deficient, 731-of E. coli unable to grow on N-acetylglucosamine,

384-of R. rubrum, thylakoid formation, 669-of S. aureus lacking extracellular nuclease activity,921

Mutants of Escherichia coli-glycolate uptake, 1088Mutations (frameshift)-in the histidine operon of Salmonella, 490Mycelial form-of P. brasiliensis, 675 -

Mycobacterium, 607-ornithinyl ester of phosphatidylglycerol, 654Mycobacterium amegmatis-purification of a D-mannose isomerase from, 777Mycobacterium tuberculosis-elongation of fatty acids, 781Mycoplasma edwardii sp. n.-classification, 346Mycoplasma felis-three-dimensional morphology, ultrastructure, and

replication, 262Mycoplasma laidlawii B-polar lipids, 72Mycoplasma pneumoniae-colony growth, 609Mycoplasma salivarium-colony growth, 609Mycoplasmas-no classification, 346Myxococcus xanthus-mRNA and germination of microcysts, 531Myxospore formation-in S. aurantiaca by monovalent cations, 325

Neisseria meningitis-catabolic activities, 127, 133-utilization of glutamate, 127-utilization of succinate, 133Neurospora crassa-conversion of histidine to hercynine, 881-enzymes in growth and morphology, 941-localization of the ,B-glucosidases, 408-morphological changes, 802-NADPH in a variety of strains, 802Nicotinamide adenine dinucleotide-from tryptophan in X. pruni, 456Nicotinamide adenine dinucleotide phosphate-in N. crassa, 802Nicotinamide deamidase-in E. coli, chromosomal location of a gene defining,

1090Nitrate-effect on electron transport system of H. parain-fluenzae, 365

Nitric oxide-production by C. nephridii, 821Nitrogen-and filamentous growth of M. rouxii, 592Nitrogenase-in Azotobacter, 292Nitrogenase complex-from C. pasteurianum, purification and properties

of the constituents, 794Nitrous oxide-production by C. nephridii, 821Nonoxidative pentose phosphate pathway-in V. alcalescens, 141Nuclease-deficient mutants-of S. aureus, 921Nucleic acid homologies-among oxidase negative Moraxella sp., 568-among Saccharomyces sp., 505Nucleic acids-33P as an indicator for 32p pulse labeling, 657

Organic substrates-regulation of autotrophic growth, 339Ornithine-anaerobic oxidation by C. sticklandii, 77Ornithinyl ester-of phosphatidylglycerol of Mycobacterium 607, 654Osmotic lysis-of A. vinelandii, 292Outgrowth-of B. megaterium spores, 58Oxidation-anaerobic, of ornithine by C. sticklandil, 77-of glutathione in E. coli by diamide, 1092Oxygen-effect on electron transport system of H. parain-fluenzae, 365

Paracoccidioides brasiliensis-glucan and chitin in the yeast phase, 636-glucans of the yeast and mycelial forms, 675Parasitic strain

f B. bacteriovorus, 989, 997PBS1-mediated transduction-in B. pumilus, 603

* *i- J. BACTERIOL.

SUBJECT INDEX

Penicillin-effect on morphology of C. psittaci, 278Penicillinase-chromosomally mediated in E. coli, 218-from E. coli, 218, 232-mediated by the R factor RI, 232-R factor-mediated in E. coli, 232Penicillinase induction-in B. cereus, 173Penicillinase synthesis-regulation, 173Penicillins-resistance of E. coli, 218, 232, 659Pentose phosphate pathway (nonoxidative)-in V. alcalescens, 141pH (low)-activation of B. cereus spores, 418Phenolic acid-synthesizing enzymes-repression and its relation to iron uptake in B.

subtilis, 181Phenyl methyl sulfonyl fluoride-inhibition of macroconidial germination, 9314C-phenylalanine-effect of thiostrepton on incorporation, 1073NP-phosphate-as indicator in pulse-labeling experiments, 657nP-phosphate labeling-of nucleic acids, 657Phosphatidylglycerol-of Mycobacterium 607, 654Photodynamic action-on native and denatured transforming DNA fromH. influenzae, 681

Phylogenetic relatedness-among oxidase-negative Moraxella sp., 568-among Saccharomyces sp., 505Phytopathogenic pseudomonads-taxonomy, 9Pili-of C. renale, 1063Pneumococci-cellular metabolism in genetic transformation, 860-that lack deoxyribonucleases, 373Pneumococcus-merodiploid ribosomal loci arising by transforma-

tion and mutation, 38Polar lipid turnover-and membrane transport in M. laidlawii B, 72Polar lipids-of M. laidlawii, 72Polyamines-requirement in bacterial division, T-16Polypeptide chain-prematurely terminated, antibodies to, 438Polyribosomes-from B. subtilis, 925Potassium-E. colt mutants dependent upon, 836Potassium-dependent mutants-of E. coli, 836Potassium ion accumulation-by S. faecalis, 152Protein-of B. subtilis, 166

-produced by strains of E. coli carrying mutations inthe,6-galactosidase structural gene, 438

-required for cell division in E. coli, 901-urea-mercaptoethanol soluble, 551Protein synthesis-and aging in vitro in R. solani, 755-and thylakoid formation in R. rubrum, 669-ribosomal, in E. coli, 574Proteins-essential to cellular competence, 860Protocatechuate pathway-in the metabolism of mandelic acid by A. niger, 786Protoplasts-of a gram-negative bacterium, 1014-of B. subtilis; effect of ,B-lysin, 314Proximal hook-isolation of in cells of B. pumilus, 250Pseudomonads-phytopathogenic, taxonomy of, 9Pseudomonas aeruginosa-detection of j-lactamase active against carbenicillin

in, 1079Pseudomonas multivorans-similarity of DNA to that of P. pseudomallel and

A. mallet, 825Pseudomonas pseudomallei-similarity of DNA to that of P. multivorans and

A. mallei, 825Pseudomonas putida-inducible hydroxyproline transport system, 948Pseudomonas species-activities of -y-butyrobetaine hydroxylase and carni-

tine dehydrogenase, 1094Purine biosynthesis-inhibition, 872Putrescine-synthesis, 725Putrescine-deficient mutants-of E. coli, 731

R factor-temperature sensitive, Rtsl, 856R factor-mediated penicillinase-in E. coli, 232R-factor-mediated piliation-regulation, 916Replacement sporulation-of B. subtilis 168, 1Replication-of M. felis, 262Resistance transfer factors-fertility regulation in, 916Reversions-of Salmonella his frameshift mutations, UV induced,

649Rhizobium japonicum-gluconate catabolism, 698Rhizoctonia solani-in vitro protein synthesis and aging, 755Rhodospirillum rubrum-thylakoid formation in mutant strains, 669Ribonucleic acid-of E. coli; incorporation of S-diazouracil-2-14C, 188-transfer in L. acidophilus, 1005

iXVOL. l01, 1970

SUBJECT INDEX

Ribonucleic acid (messenger)-and germination of M. xanthus microcysts, 531Ribonucleic acid (transfer) methylases-inhibitor of in D. discoideum, 705Ribosomal protein synthesis-in E. coli, 574Ribosome-membrane complex-of E. coli, effect of lysozyme, 584Ribosome units (SOS)-detection of biological activity, 1073

Saccharomyces-control of lysine biosynthesis, 108Saccharomyces cerevisiae-double-bond requirement in a fatty acid desaturasemutant, 160-fatty acid desaturase mutant, 160Saccharomyces species-nucleic acid homologies, 505Salmonella his frameshift mutations-UV-induced reversions, 649Salmonella typhimurium-ICR-induced frameshift mutations in the histidine

operon, 490L-Serine dehydratase-from B. alvei, 813Slime mold-inhibitor of tRNA methylases in, 705Slime-producing bacteria-in acid mine water, 982Sodium and hydrogen ions-extrusion by S. faecalis, 152Spheroplast membrane-effect of col E2, 102Spore (clostridial)-germination, 1038Spore germination-biochemical changes, 931Spore germination and fungal sporulation-biochemical changes, 931Spores-dormant, of A. oryzae, induction of enzymes, 1070-of B. cereus, chemical composition of exosporium,

196-of B. cereus; low pH activation, 418-of B. megaterium; effects of temperature, 58-of B. thuringiensis, 551-streptomycete, surface structure, 318Sporogenesis-in Bacillus sp., 24Sporulating organisms-isolation and characterization of dihydrodipicolinic

acid synthetase, 118Sporulation and growth-of B. cereus, 483Sporulation mutant-of B. subtilis, 1027Sporulation of yeast-carbohydrate accumulation, 53Sporulation (replacement)-of B. subtilis 168, 1Staphylococcus aureus-mutants lacking extracellular nuclease activity, 921-nuclease-deficient mutants, 921

Stigmatella aurantiaca (Myxobacterales)-induction of myxospore formation by monovalent

cations, 325Streptococcus faecalis-extrusion of sodium and hydrogen ions, 152-fructose-1, 6-diphosphate activated lactate dehy-

drogenase from, 717-potassium ion accumulation, 152Streptococcus mitis-role of glycogen in survival, 1083Streptomycete spores-surface structure, 318Substrates (organic)-regulation of autotrophic growth, 339Succinate-utilization in N. meningitis, 133Succinyl blocking groups-in diaminopimelic acid biosynthesis, 323Sulfur globules-of Chromatium, 464Supersuppressor genes-and lysine biosynthesis, 108Synthesis-of bacteriochlorophyll, fatty acid, and protein in

R. rubrum, 669-of protein in vitro in R. solani, 755-of putrescine, 725-of ribosomal protein in E. coli, 574Synthesis (macromolecular)-during microcycle sporogenesis of B. cereus T, 24

Taxonomy-of C. acnes, 392-of phytopathogenic pseudomonads, 9Temperature-effect on activation, germination, and outgrowth of

B. megaterium spores, 58Temperature-sensitive mutants-for initiation of germination, 327Temperature-sensitive R factor-Base composition of DNA, 856Thermophilic Bacillus sp.-fatty acid composition of lipid extracts, 145Thermostable aldolase-from T. aquaticus, 541Thermus aquaticus-thermostable aldolase, 541Thiazole biosynthesis-in E. coli, effect of glycine, 1076Thiobacilli-comparative ultrastructure, 618Thiostrepton-inhibitor of 50S ribosome subunit function, 1073Thylakoid-formation in R. rubrum, 669Thymidine and thymine uptake-by H. influenzae, 525Thymine and thymidine uptake-by H. influenzae, 525Toxic glycolipid-of C. diphtheriae, action on mitochondrial structureand function, 709

x J. BACTERIOL.

SUBJECT INDEX

Transduction-in C. renale, 1086-PBS1 mediated, in B. pumilus, 603Transfer-of RNA from nucleus to cytoplasm, 1005Transfer ribonucleic acid methylases-inhibitor of in D. discoideum, 705Transformation-energy-dependent step, 35-of B. subtilis with single-stranded DNA, 844Transforming activity-of complementary strands of D. pneumoniae DNA,

361Transforming deoxyribonucleic acid-decrease in integration, UV induced, 444-early energy-dependent step in entry, 35-from H. influenzae, 681-repair, 808Transport-of glucose-l-phosphate in A. tumefaciens, 685-of hexose-6-phosphate in E. coli, 470-of hydroxyproline in P. putida, 948Transport capacity-of cells, mureinoplasts, and protoplasts of a gram-

negative bacterium, 1014Transport (membrane)-and polar lipid turnover in M. la;dlawii B, 72Trifluoroleucine-resistant yeast mu' nts, 1081Tryptophan-conversion to NAD, 456Tryptophan auxotroph-of B. subtilis, 350Tryptophan biosynthesis-in E. coli, 202, 209Tryptophan metabolism-regulation in X. pruni, 456Tryptophan operon-mechanism of 3-methylanthranilic acid derepres-

sion, 209-regulation in E. coli, 202, 209Tryptophanase-from B. alvei, 813Tryptophanless death-in B. subtilis, 350, 355

-in A. vinelandii; correlation with nitrogen source forgrowth, 286

-of M. felis, 262-of the cell envelope of E. coli B after freeze-etching,

297-of the thiobacilli, 618

Ultraviolet-and reversions of Salmonella his frameshift muta-

tions, 649Ultraviolet-irradiated deoxyribonucleic acid-repair in H. influenzae, 808Ultraviolet irradiation-effect on integration of H. influenzae transformingDNA, 444

Uptake-of thymine and thymidine by H. influenzae, 525Urea-mercaptoethanol-soluble protein-from spores of B. thuringiensis, 551Uridine-incorporation into B. subtilis DNA, 330-incorporation into SPP1 bacteriophage DNA, 330Uridine diphosphate-in wild-type E. coli, 959Uridine diphosphate-glucose dehydrogenase-synthesis in E. coli K-12, 959Uridine diphosphate-glucuronic acid-accumulation in E. coli K-12, 959

Vegetative cells-sporulation in B. subtilis, 1Veillonella alcalescens-multiple impairment of glycolysis, 138-nonoxidative pentose phosphate pathway, 141

X-ray diffraction studies-of sulfur globules of Chromatium, 464Xanthomonas pruni-and tryptophan metabolism, 456

Yeast form-of P. brasiliensis, 675Yeast mutants-trifluoroleucine resistant, 1081Yeast sporulation-carbohydrate accumulation, 53

VOL. 101 ) 1970 Xi

INDEX TO DATE OF ISSUEMonth Date of Issue Pages

January 1/27/70 - 1-332February 2/11/70 333-658March 3/6/70 659-1096

JOURNAL

OF

BACTERIOLOGY

VOLUME 101

BALTIMORE, MD. 21202

1970

JOURNAL OF BACTERIOLOGYVOLUME 101

EDITORIAL BOARD

L. LEON CAMPBELL, Editor-in-Chief (1975)University of Illinois, UrbanaL. S. BARON, Editor (1970)

Walter Reed Army Institute of ResearchWashington, D.C.

R. D. DeMOSS, Editor (1970)University oJ Illinois, Urbana

ALLEN G. MARR, Editor (1973)University oj California, Davis

Howard l. Adler (1970)R. L. Anderson (1972)W. Lane Barksdale (1972)Robert W. Bernlohr (1970)S. Gaylen Bradley (1969)Arnold F. Brodie (1970)Alan Campbell (1972)V. P. Cirfllo (1970)Royston C. Clowes (1972)Wallace A. Clyde, Jr. (1970)V. W. Cochane (1972)S. F. Conti (1969)Roy Curtiss III (1972)A. L. Demain (1972)John A. DeMoss (1972)Martin Dworkin (1970)Ellis Englesberg (1972)Stanley Falkow (1972)Leonard Frank (1970)Martin Freundlich (1970)Herman Friedman (1972)A. T. Giwesan (1972)Joseph S. Gots (1972)

Harlyn 0. Halvorson (1972)F. M. Harold (1970)Leonard Hayffick (1972)George Hegeman (1971)D. S. Hoare (1971)John G. Holt (1972)Stanley C. Holt (1971)John Ingraham (1970)John H. Law (1971)Edward R. Leadbetter (1970)Hillel S. Levinson (1972)H. C. Lichstein (1970)W. R. Lockhart (1972)Donald G. Lundgren (1971)Manley Mandel (1972)Paul Margolin (1972)Julius Marmur (1971)Robert G. Martin (1971)M. L. Morse (1972)M. Nomura (1971)Richard P. Novick (1970)Kenneth Paigen (1969)Harry D. Peck (1970)

H. J. Phaff (1972)Jack Preiss (1971)H. V. Rickenberg (1970)M. R. J. Salton (1969)Moselio Schaechter (1970)David Schlessinger (1971)W. R. Sistrom (1972)Paul F. Smith (1970)Roger Y. Stanier (1970)M. P. Starr (1970)Bernard S. Strauss (1972)Noboru Sueoka (1972)Paul S. Sypherd (1971)A. L. Taylor (1971)H. E. Umbarger (1970)Arthur Weissbach (1971)N. E. Welker (1970)R. W. Wheat (1972)Helen R. Whiteley (1972)Meyer J. Wolin (1970)Barbara E. Wright (1971)Frank E. Young (1970)Stanley A. Zahler (1971)

ROBERT A. DAY, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIOE. M. FOSTER, President (1969-1970)

DONALD E. SHAY, SecretaryThe Jouirnal of Bacteriology, a publication of the American

Society for Microbiology, 4715 Cordell Ave., Bethesda, Md.20014, is devoted to the advancement and dissemination of funda-mental knowledge concerning bacteria and other microorganisms.The Journal is published monthly, and the twelve numbers aredivided into four volumes per year. The subscription price is$40.00 (Postal Union Countries, $42; other foreign, $43) peryear. Single copies are $4.00 (foreign, $4.25). Members of theAmerican Society for Microbiology, may receive the Journal aspart of their dues. Correspondence relating to reprints, defectivecopies, availability of back issues, lost or late proofs, disposi-tion of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Office, 4715 CordellAve., Bethesda, Md. 20014. Correspondence from ASM mem-

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bers relating to membership dues, member subscriptions,changes of address, incorrect address, incorrect journals, etc.,should be directed to the American Society for Microbiology,1913 I St., N.W., Washington, D.C. 20006. Nonmembers ofthe Society should write to The Williams & Wilkins Co., 428 E.Preston St., Baltimore, Md. 21202, regarding institution of non-member subscriptions and nonreceipt of journals. Publishedmonthly by the ASM at 428 E. Preston St., Baltimore, Md.21202.

Second class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices.Made in the United States of America.Copyright 0 1970, American Society for Microbiology.All Rights Reserved

Volume 101 Contents for January Number 1Taxonomy and Ecology

Taxonomy of Phytopathogenic Pseudomonads. D. C. SANDS, M. N. SCHROTH, ANDD. C. HILDEBRAND....................................................... 9

Morphology and UltrastructureMorphogenesis and Fine Structure of Leucothrix mucor and Effects of Calcium De-

ficiency. JAMES E. SNELLEN AND H. D. RAJ.................................... 240Differentiation Within the Bacterial Flagellum and Isolation of the Proximal Hook.

DINAH ABRAM, J. R. MITCHEN, HENRY KOFFLER, AND A. E. VATTER............ 250Three-Dimensional Morphology, Ultrastructure, and Replication of Mycoplasma felis.

EDWIN S. BOATMAN AND GEORGE E. KENNY................................. 262Electron Microscopic Observations on the Effects of Penicillin on the Morphology of

Chlamydia psittaci. A. MATSUMOTO AND G. P. MANIRE......................... 278Correlation of Ultrastructure in Azotobacter vinelandii with Nitrogen Source for

Growth. JOEL OPPENHEIM AND LEON MARCUS............................... 286Ultrastructure of the Cell Envelope of Escherichia coli B After Freeze-Etching. N.

NANNINGA.............................................................. 297Structure of Escherichia coli After Freeze-Etching. M. E. BAYER AND C. C. REMSEN..... 304Effect of ,3-Lysin on Isolated Cell Walls and Protoplasts of Bacillus subtilis. AUDRIA

MATHESON AND DAVID M. DONALDSON............................ 314Surface Structure of Streptomycete Spores as Revealed by Negative Staining and

Freeze-Etching. H. WILDERMUTH ......... ................................. 318

Genetics and Molecular BiologyEarly Energy-Dependent Step in the Entry of Transforming Deoxyribonucleic Acid.

NORMAN STRAUSS.........................................................35Merodiploid Ribosomal Loci Arising by Transformation and Mutation in Pneumococ-

cus. ARNOLD W. RAVIN AND ELAINE A. TAKAHASHI.......................... 38Effects of Supersuppressor Genes on Enzymes Controlling Lysine Biosynthesis in

Saccharomyces. THORSTEN A. FJELLSTEDT AND MAURICE OGUR................ 108Incorporation of 5-Methyl- and 5-Hydroxytryptophan into the Protein of Bacillus

subtilis. SERGIO BARLATI AND ORIO CIFERRI.................................. 166Regulation of Penicillinase Synthesis: Evidence for a Unified Model. JOHN IMSANDE .... 173Incorporation of Uridine Into Bacillus subtilis and SPP1 Bacteriophage Deoxyribonu-

cleic Acid. SERGIO BARLATI............................................... 330

Physiology and MetabolismReplacement Sporulation of Bacillus subtilis 168 in a Chemically Defined Medium.

ROBERT F. RAMALEY AND LINDA BURDEN................................... 1Macromolecular Synthesis During Microcycle Sporogenesis of Bacillus cereus T. IAN

MACKECHNIE AND R. S. HANSON.......................................... 24Carbohydrate Accumulation During the Sporulation of Yeast. ROBERT ROTH........ 53Effects of Temperature on Activation, Germination, and Outgrowth of Bacillus

megaterium Spores. HILLEL S. LEVINSON AND MILDRED T. HYATT.............. 58Fatty Acid Toxicity and Methyl Ketone Production in Aspergillus niger. HAROLD L.

LEWIS AND DENNIS W. DARNALL................................. ........ 65Metabolic Turnover of the Polar Lipids of Mycoplasma laidlawii Strain B. RONALD N.

MCELHANEY AND MARK E. TOURTELLOTTE.................................. 722,4-Diaminovaleric Acid: an Intermediate in the Anaerobic Oxidation of Ornithine

by Clostridium sticklandii. JOHN K. DYER AND RALPH N. COSTILOW............ 77Lipids of Bacteroides melaninogenicus. VICTOR RIZZA, ANNE N. TUCKER, AND DAVID

C. WHITE............................................................... 84Electromechanical Interactions in Cell Walls of Gram-Positive Cocci. LI-TsE OU 92

AND ROBERT E. MARQUIS.... .............................................

Stabilizing Effect of Colicin E2 on the "Spheroplast Membrane." K. NoSE, M. ONO,AND D . MIZUNO ........................................................ 102

xv

CONTENTS

Catabolic Activities of Neisseria meningitidis: Utilization of Glutamate. Louis P. MAL-LAVIA AND EMILIO WEISS.................................................. 127

Catabolic Activities of Neisseria meningitidis: Utilization of Succinate. EMILIO WEISS. .. 133Multiple Impairment of Glycolysis in Veillonella alcalescens. R. N. MICHAUD AND E. A.

DELWICHE........................................ .................... 138Nonoxidative Pentose Phosphate Pathway in Veillonella alcalescens. R. N. MICHAUD,

J. A. CARROW, AND E. A. DELWICHE.141Fatty Acid Composition of Lipid Extracts of a Thermophilic Bacillus Species. HARLOW

H. DARON.............................................................. 145Extrusion of Sodium and Hydrogen Ions as the Primary Process in Potassium Accumu-

lation by Streptococcusfaecalis. F. M. HAROLD, J. R. BAARDA, AND E. PAVLASOVA. 152Incorporation of 5-Diazouracil-2-'4C into Ribonucleic Acids of Escherichia coli During

Division Inhibition. EDWARD PREVIC AND GEORGE FISTER .................... 188Chemical Composition of Exosporium from Spores of Bacillus cereus. L. L. MATZ, T.

CABRERA BEAMAN, AND PHILIPP GERHARDT................................. 196Regulation of the Escherichia coli Tryptophan Operon by Early Reactions in the

Aromatic Pathway. WILLIAM A. HELD AND OLIVER H. SMITH... 202Mechanism of 3-Methylanthranilic Acid Derepression of the Tryptophan Operon in

Escherichia coli. WILLIAM A. HELD AND OLIVER H. SMITH... 209Bacterial Distribution of the Use of Succinyl and Acetyl Blocking Groups in Diaminopi-

melic Acid Biosynthesis. STEVEN WEINBERGER AND CHARLES GILVARG.323Induction of Myxospore Formation in Stigmatella aurantiaca (Myxobacterales) by

Monovalent Cations. HANS REICHENBACH AND MARTIN DWORKIN.325Biochemical Studies of Bacterial Sporulation and Germination. XXI. Temperature-

Sensitive Mutants for Initiation of Germination. JAMES C. VARY AND ARTHURKORNBERG......................................................... 327

Enzymology

Partial Purification and Characterization of Dihydrodipicolinic Acid Synthetase fromSporulating Bacillus megaterium. FRANCIS H. WEBSTER AND RICHARD V. LECHO-WICH.118

Double-Bond Requirement in a Fatty Acid Desaturase Mutant of Saccharomyces cere-visiae. BERNADINE J. WISNIESKI, ALEC D. KEITH, AND MICHAEL R. RESNICK. 160

Repression of Phenolic Acid-Synthesizing Enzymes and Its Relation to Iron Uptake inBacillus subtilis. D. N. DOWNER, W. B. DAVIS, AND B. R. BYERS.181

Resistance of Escherichia coli to Penicillins. VI. Purification and Characterization ofthe Chromosomally Mediated Penicillinase Present in ampA-Containing Strains.E. B6RJE LINDSTR6M, HANS G. BOMAN, AND BARBARA B. STEELE.218

Resistance of Escherichia coli to Penicillins. VII. Purification and Characterization of aPenicillinase Mediated by the R Factor RI. R. CHRISTER LINDQVIST AND KURTNORDSTR6M . ............................................................ 232

Properties of a Soluble Nitrogenase in Azotobacter. JOEL OPPENHEIM, ROBERT J.FISHER, P. W. WILSON, AND LEON MARCUS.292

Volume 101 Contents for February Number 2Taxonomy and Ecology

Proposal for Classifying Strain PG-24 and Related Canine Mycoplasmas as Myco-plasma edwardii sp. n. JOSEPH G. TULLY, MICHAEL F. BARILE, RICHARD A.DEL GIUDICE, THEODORE R. CARSKI, DONALD ARMSTRONG, AND SHMUEL RAZIN 346

Differentiation of Two Types of Corynebacterium acnes. J. G. Voss.............. 392Nucleic Acid Homologies Among Species of Saccharomyces. J. N. BICKNELL ANDHOWARD C. DOUGLAS.505

Nucleic Acid Homologies Among Oxidase-Negative Moraxella Species. JOHN L.JOHNSON, ROBERT S. ANDERSON, AND ERLING J. ORDAL........................ 568

XVi

CONTENTS

Morphology and UltrastructureX-Ray Diffraction Studies of the Sulfur Globules Accumulated by Chromatium Species.

G. J. HAGEAGE, JR., E. D. EANES, AND R. L. GHERNA.......................... 464Mycoplasma pneumoniae and Mycoplasma salivarium: Electron Microscopy of ColonyGrowth in Agar. DENNIS L. KNUDSON AND RODERICK MACLEOD.............. 609

Comparative Ultrastructure of the Thiobacilli. J. M. SHIVELY, G. L. DECKER, ANDJ. W. GREENAWALT........................................................ 618

Ultrastructural Changes During the Yeastlike to Mycelial-Phase Conversion of Blasto-myces dermatitidis and Histoplasma capsulatum. ROBERT G. GARRISON, JAMES W.LANE, AND MARVIN F. FIELD................................................ 628

Chemical Morphology of Glucan and Chitin in the Cell Wall of the Yeast Phase ofParacoccidioides brasiliensis. L. M. CARBONELL, F. KANETSUNA, AND F. GIL ... 636

Model for Cell Wall Growth of Streptococcus faecalis. MICHAEL L. HIGGINS ANDGERALD D. SHOCKMAN..................................................... 643

Genetics and Molecular BiologyCorrelation of Melting Temperature and Cesium Chloride Buoyant Density of Bac-

terial Deoxyribonucleic Acid. M. MANDEL, LEVI IGAMBI, JANET BERGENDAHL,J. L. DODSON, JR., AND E. SCHELTGEN....................................... 333

Tryptophanless Death in Bacillus subtilis. IRENE MAJERFELD, SERGio BARLATI, ANDORIO CIFERRI............................................................. 350

Partial Characterization of the Factor Responsible for Tryptophanless Death inBacillus subtilis. SERGIO BARLATI AND IRENE MAJERFELD...................... 355

Competent Diplococcus pneumoniae Accept Both Single- and Double-Stranded De-oxyribonucleic Acid. RAYMOND MIAO AND W. R. GUILD.................... 361

Mutants of Diplococcus pneumoniae that Lack Deoxyribonucleases and Other Activi-ties Possibly Pertinent to Genetic Transformation. SANFORD LACKS............ 373

In Vivo Suppression of Coding Associated with Bacteriophage-Induced S-Adenosyl-methionine Hydrolase. P. W. SIERSMA AND S. LEDERBERG..................... 398

f8-Galactosidase: Isolation of and Antibodies to Incomplete Chains. ALAN P. BERG,AUDREE V. FOWLER, AND IRVING ZABIN.............................. 438

Ultraviolet-Induced Decrease in Integration of Haemophilus influenzae TransformingDeoxyribonucleic Acid in Sensitive and Resistant Cells. AMIR MUHAMMED ANDJANE K. SETLOW ......................................................... 444

Increase in Lytic Activity in Competent Cells of Bacillus subtilis After Uptake of De-oxyribonucleic Acid. CHARLES R. STEWART AND JULIUS MARMUR.............. 449

ICR-Induced Frameshift Mutations in the Histidine Operon of Salmonella. NICOLES. OESCHGER AND PHILIP E. HARTMAN........................................ 490

Defined Medium for Growth of Haemophilus influenzae. ROGER M. HERRIOTT,ELEANOR Y. MEYER, MARCELLA VOGT, AND MICHAELA MODAN................ 513

Defined Nongrowth Media for Stage II Development of Competence in Haemophilusinfluenzae. R. M. HERRIOTT, E. M. MEYER, AND M. VOGT .................... 517

Thymine and Thymidine Uptake by Haemophilus influenzae and the Labeling of De-oxyribonucleic Acid. JANICE M. CARMODY AND ROGER M. HERRIOTT.......... 525

Stable Messenger Ribonucleic Acid and Germination of Myxococcus xanthus Micro-cysts. W. SCOTT RAMSEY AND MARTIN DWORKIN............................. 531

Regulation of Ribosomal Protein Synthesis in Escherichia coli. R. J. HARVEY ...... 574Interaction of Ribosomes and the Cell Envelope of Escherichia coli Mediated by

Lysozyme. DAVID PATTERSON, MARK WEINSTEIN, RALPH NIXON, AND DAVIDGILLESPIE ................................................................ 584

Genetic Analysis in Bacillus pumilus by PBSl-Mediated Transduction. PAUL S.LOVETT AND FRANK E. YOUNG.............................................. 603

Ultraviolet-Induced Reversions of Salmonella his Frameshift Mutations. DUSAN T.KANAZIR, PHILIP E. HARTMAN, AND DRAGUTIN SAVIC.......................... 649

Use of 33P as an Indicator for 32p Pulse Labeling of Nucleic Acids in Bacterial Cul-tures. B. JORDAN......................................................... 657

x*ii

CONTENTS

Physiology and MetabolismAutotrophic and Heterotrophic Metabolism of Hydrogenomonas: Regulation of

Autotrophic Growth by Organic Substrates. PHILIP E. STUKUS AND B. T.DECICCO................................................................. 339

Effect of Nitrate, Fumarate, and Oxygen on the Formation of the Membrane-BoundElectron Transport System of Haemophilus parainfluenzae. PETER R. SINCLAIR ANDDAVID C. WHITE......................................................... 365

Amino Sugar Sensitivity in Escherichia coli Mutants Unable to Grow on N-Acetyl-glucosamine. NAOMI J. BERNHEIM AND WALTER J. DOBROGOSZ................ 384

Low-pH Activation of Bacillus cereus Spores. GABRIELA ISSAHARY, Z. EVENCHIK,AND A. KEYNAN................................... .........................418Hormonal Interactions in Mucor mucedo and Blakeslea trispora. H. VAN DEN ENDE,A. H. C. A. WIECHMANN, D. J. REYNGOUD, AND T. HENDRIKS................... 423

Characterization of Injury Incurred by Escherichia coli upon Freeze-Drying. T. J.SINSKEY AND G. J. SILVERMAN....................................... ....... 429

Regulation of Enzymes Involved in the Conversion of Tryptophan to NicotinamideAdenine Dinucleotide in a Colorless Strain of Xanthomonas pruni. ALBERT T.BROWN AND CONRAD WAGNER.............................................. 456

Compartmentation in the Induction of the Hexose-6-Phosphate Transport System ofEscherichia coli. HERBERT H. WINKLER.................................... 470

Lipid Composition of Bacillus cereus During Growth and Sporulation. DENNIS R.LANG AND D. G. LUNDGREN...................................... ...........483Urea-Mercaptoethanol-Soluble Protein from Spores of Bacillus thuringiensis andOther Species. H. J. SOMERVILLE, F. P. DELAFIELD, AND S. C. RITTENBERG ...... 551

Growth in Volume of Euglena gracilis During the Division Cycle. E. S. KEMPNERAND A. G. MARR.......................................................... 561

Filamentous Growth of Mucor rouxii Under Nitrogen. GARY W. ELMER AND WALTERJ. NICKERSON............................................................. 592

Nutritional Requirements for Growth and Yeastlike Development of Mucor rouxiiUnder Carbon Dioxide. GARY W. ELMER AND WALTER J. NICKERSON .......... 595

Cytochrome Composition of Some Members of the Actinoplanaceae. A. DOMNASAND NEIL G. GRANT...................................................... 652

On the Ornithinyl Ester of Phosphatidylglycerol of Mycobacterium 607. G. K.KHULLER AND D. SUBRAHMANYAM........................................... 654

EnzymologyLocalization of the 3-Glucosidases in Neurospora crassa. BRUCE M. EBERHART ANDRETA S. BECK............................................................. 408

Physiological and Kinetic Studies with Anthranilate Synthetase of Bacillus alvei.ANTHONY CATENA AND R. D. DEMOSS....................................... 476

Thermostable Aldolase from Thermus aquaticus. HUDSON FREEZE AND THOMAS D.BROCK......................... ........................................... 541

Volume 101 Contents for March Number 3

Taxonomy and EcologyReexamination of the Association Between Melting Point, Buoyant Density, and

Chemical Base Composition of Deoxyribonucleic Acid. J. DE LEY........... 738Comparative Allostery of 3-Deoxy-D-arabino-Heptulosonate-7-Phosphate Synthetase

as a Molecular Basis for Classification. Roy A. JENSEN AND SHERRY L. STEN-MARK .. 763

Nucleic Acid Similarities Among Pseudomonas pseudomallei, Pseudomonas multi-vorans, and Actinobacillus mallei. M. ROGUL, J. J. BRENDLE, D. K. HAAPALA,AND A. D. ALEXANDER................................................... 827

Aerobic Heterotrophic Bacteria Indigenous to pH 2.8 Acid Mine Water: Micro-scopic Examination of Acid Streamers. PATRICK R. DUGAN, CAROL B. MAC-MILLAN; AND ROBERT M. PFISTER......................................... 973

xviii

CONTENTS

Aerobic Heterotrophic Bacteria Indigenous to pH 2.8 Acid Mine Water: Predomi-nant Slime-Producing Bacteria in Acid Streamers. PATRICK R. DUGAN, CAROLB. MACMILLAN, AND ROBERT M. PFISTER...... ............ 982

Morphology and UltrastructureCell Wall Glucans of the Yeast and Mycelial Forms of Paracoccidioides brasiliensis.

FUMINORI KANETSUNA AND LuIs M. CARBONELL............................. 675Facultatively Parasitic Strain of Bdellovibrio bacteriovorus. D. L. DIEDRICH, C. F.

DENNY, T. HASHIMOTO, AND S. F. CONTI................................... 989Ultrastructure and Cell Division of a Facultatively Parasitic Strain of Bdellovibrio

bacteriovorus. JEFFREY C. BURNHAM, T. HASHIMOTI, AND S. F. CONTI......... 997Ultrastructural Changes Associated with Germination and Outgrowth of an Append-

age-Bearing Clostridial Spore. WILLIAM A. SAMSONOFF, T. HASHIMOTO, ANDS. F. CONTI. .................... .................................. .. 1038

Some Properties of the Pili of Corynebacterium renale. RYO YANAGAWA AND KOICHIOTSUKI. .. .. .. .. .. .. .. .. .. ...... .. ... ....... .... ... ... ... ... .. 1063

Genetics and Molecular BiologyBacteriochlorophyll, Fatty-Acid, and Protein Synthesis in Relation to Thylakoid

Formation in Mutant Strains of Rhodospirilluim rubrum. J. OELZE, J. SCHROEDER,AND G. DREWS......................................................... 669

Photodynamic Action on Native and Denatured Transforming Deoxyribonucleic Acidfrom Haemophilus influenzae. MANUEL PONCE-DE LEON AND EMILIANO CABRERA-JUAREZ....................................... 681

In Vitro Protein Synthesis and Aging in Rhizoctonia solani. T. G. OBRIG AND DAVIDGOTTLIEB............................ .....................................755

Requirement of Polyamines for Bacterial Division. MASAYORI INOUYE AND ARTHURB. PARDEE.............................................................. 770

Repair of Ultraviolet-Irradiated Transforming Deoxyribonucleic Acid in Haemophilusiqifluenzae. KENNETH L. BEATTIE AND JANE K. SETLOW....................... 808

Potassium-Dependent Mutants of Escherichia coli K-12. WOLFGANG EPSTEIN ANDMARTINDAVIES...836MARTIN DAVIES

..Nature of the Ethylenediaminetetraacetic Acid Requirement for Transformation ofBacillus subtilis with Single-Stranded Deoxyribonucleic Acid. M. J. TEVETHIAAND M. MANDEL........................................................ 844

Quantitative Autoradiographic Study of Competence and Deoxyribonucleic AcidIncorporation in Bacillus subtili,s. SERGIO SOMMA AND MARIO POLSINELLI ...... 851

Base Composition of Deoxyribonucleic Acid of the Temperature-Sensitive Kana-mycin-Resistant R Factor, Rtsl. NOBUICHI GOTO, YOKO YOSHIDA, YOSHIROTERAWAKI, RINTARO NAKAYA, AND KENJI SUZUKI........................... 856

Cellular Metabolism in Genetic Transformation of Pneumococci: Requirement forProtein Synthesis During Induction of Competence. ALEXANDER TOMASZ..... 860

Accumulation of a Protein Required for Division During the Cell Cycle of Escherichiacoli. HELENE S. SMITH AND ARTHUR B. PARDEE ............................ 901

Fertility Regulation in F-Like Resistance Transfer Factors. DAVID L. HOAR........ 916Isolation of Mutants of Staphylococcus aureus Lacking Extracellular Nuclease Ac-

tivity. GILBERT S. OMENN AND JOHN FRIEDMAN............................ 921Polyribosomes from Bacillus subtilis During Amino Acid Starvation in the Presence

and in the Absence of Actinomycin. SERGIO BARLATI ...................... 925Control of Uridine Diphosphate-Glucose Dehydrogenase Synthesis and Uridine

Diphosphate-Glucuronic Acid Accumulation by a Regulator Gene Mutation inEscherichia coli K-12. MICHAEL M. LIEBERMAN, ANNA SHAPARIS, AND ALVINMARKOVITZ ............................................................. 959

Derepression of Guanosine Diphosphate-Mannose Pyrophosphorylase by Mutationsin Two Different Regulator Genes Involved in Capsular Polysaccharide Synthe-sis in Escherichia coli K-12. MICHAEL M. LIEBERMAN AND ALVIN MARKOVITZ.. 965

Effect of Actinomycin D on the Transfer of Ribonucleic Acid from Nucleus to Cyto-plasm in Lactobacillus acidophilus. NING-CHUN CHAI AND K. G. LARK....... 1005

X1X

Thiostrepton, an Inhibitor of 50S Ribosome Subunit Function. B. WEISBLUM ANDV. DEMOHN................................ ..............................1073Simple Selection for End-Product Inhibitor-Insensitive Mutants in Yeast. HOWARDBUSSEY.................................... 1081

Generalized Transduction in Corynebacterium renale. K. HIRAI AND R. YANAGAWA 1086Chromosomal Location of a Gene Defining Nicotinamide Deamidase in Escherichia

coli. ELLEN S. DICKINSON AND T. K. SUNDARAM........................... 1090

Physiology and MetabolismResistance of Escherichia coli to Penicillins. VIII. Physiology of a Class II Ampicillin-

Resistant Mutant. KURT NORDSTR6M, LARS G. BURMAN, AND KERSTIN G.ERIKSSON-GRENNBERG....................................................659Active Transport of Glucose-l-Phosphate in Agrobacterium tumefaciens. SAKUZOFUKUI AND SACHIO MIYAIRI .............................................685Gluconate Catabolism in Rhizobium japonicum. BERNARD B. KEELE, JR., PAT B.HAMILTON, AND GERALD H. ELKAN........................................ 698

Action of a Toxic Glycolipid of Corynebacterium diphtheriae on Mitochondrial Struc-ture and Function. MASAHIKO KATO...................................... 709

Isolation and Characterization of a Mutant of Escherichia coli Blocked in the Synthe-sis of Putrescine. I. N. HIRSHFIELD, H. J. ROSENFELD, Z. LEIFER, AND W. K.MAAS............................................................. 725

Isolation of Conditionally Putrescine-Deficient Mutants of Escherichia coli. DAVIDR. MORRIS AND CAROLINE M. JORSTAD............................ 731

Elongation of Fatty Acids in Mycobacterium tubercu/iosis. LYNN WANG, TAKASHIKUSAKA, AND DEXTER S. GOLDMAN........................................ 781

Involvement of the Protocatechuate Pathway in the Metabolism of Mandelic Acid byAspergillus niger. M. JAMALUDDIN, P. V. SUBBA RAO, AND C. S. VAIDYANATHAN 786

Correlation Between Reduced Nicotinamide Adenine Dinucleotide Phosphate Levelsand Morphological Changes in Neurospora crassa. STUART BRODY........... 802

Production of Nitric Oxide and Nitrous Oxide During Denitrification by Coryne-bacterium nephridii. E. D. RENNER AND GEORGE E. BECKER.................. 821

Inhibition of De Novo Purine Biosynthesis and Interconversion by 6-Methylpurine inEscherichia coli. CHARLES E. BENSON, SAMUEL H. LOVE, AND CHARLES N. REMY 872

Conversion of Histidine to Hercynine by Neurospora crassa. VERNON N. REINHOLD,YOSHINORI ISHIKAWA, AND DONALD B. MELVILLE............................ 881

Pathways of Anaerobic Acetate Utilization in Escherichia coli and Aerobacter cloacae.THOMAS E. HIGGINS AND MARVIN J. JOHNSON .............................. 885

Correlation of Bacterial Lipid Composition with Antibiotic Resistance. JUNE K.DUNNICK AND WILLIAM M. O'LEARY ................................. .... 892

Biochemical Changes During Fungal Sporulation and Spore Germination. I. PhenylMethyl Sulfonyl Fluoride Inhibition of Macroconidial Germination in Micro-sporum gypseum. T. J. LEIGHTON AND J. J. STOCK.......................... 931

Properties of the Inducible Hydroxyproline Transport System of Pseudomonas putida.ROSA M. GRYDER AND ELIJAH ADAMS....................948.................

Stability and Comparative Transport Capacity of Cells, Mureinoplasts, and TrueProtoplasts of a Gram-Negative Bacterium. I. W. DE VOE, J. THOMPSON, J. W.COSTERTON, AND ROBERT A. MACLEOD..................................... 1014

Isolation and Properties of a Temperature-Sensitive Sporulation Mutant of Bacillussubtilis. J. SZULMAJSTER, CELINE BONAMY, AND J. LAPORTE ...... ............ 1027

Growth, Sporulation, and Enzyme Defects of Glucosamine Mutants of Bacillussubtilis. ELISABETH B. FREESE, ROGER M. COLE, WALTHER KLOFAT, AND ERNSTFREESE ..... .. ....... .... ...... .. ..... ..... .. .. .. .......... ...... .. 1046

Effect of Glycine on Thiazole Biosynthesis in Escherichia coli. AKIO IWASHIMA ANDYOSHITSUGU NOSE............................................. 1076

Role of Glycogen in Survival of Streptococcus mitis. J. VAN HOUTE AND H. M.JANSEN............................................. 1083

Glycolate Uptake by Mutant Strains of Escherichia coli K-12. L. N. ORNSTON ANDM. K. ORNSTON............................................. 1088

CONTENTSxx

CONTENTS

Effect of the Thiol-Oxidizing Agent Diamide on the Growth of Escherichia coli.RICHARD WAX, EUGENE ROSENBERG, NECHAMA S. KoSOWER, AND EDWARD M.KOSOWER ....................................................1092

Enzymologya-3-Ketoglucosidase of Agrobacterium tumefaciens. KOICHI HAYANO AND SAKUZO

FUKUI................................................................. 692Inhibitor of Transfer Ribonucleic Acid Methylases in the Differentiating Slime Mold

Dictyostelium discoideum. OPENDRA K. SHARMA AND ERNEST BOREK.......... 705Purification and Properties of a Fructose- 1,6-Diphosphate-Activated Lactate De-

hydrogenase from Streptococcus faecalis. CHARLES L. WITTENBERGER ANDNOREEN ANGELO...........................7............................ 717

Purification of a D-Mannose Isomerase from Mycobacterium smegmatis. ANNHEY-FERGUSON AND ALAN D. ELBEIN...................................... 777

Purification and Properties of the Constituents of the Nitrogenase Complex fromClostridium pasteurianum. JEAN-PAUL VANDECASTEELE AND R. H. BURRIS..... 794

Physiological Comparison of L-Serine Dehydratase and Tryptophanase from Bacillusalvei. S. K. GRIFFITHS AND R. D. DEMOSS................................ 813

D-Apiose Reductase from Aerobacter aerogenes. DONNA L. NEAL AND PAUL K.KINDEL................................................................. 910

Role of Enzymes in Growth and Morphology of Neurospora crassa: Cell-Wall-BoundEnzymes and Their Possible Role in Branching. P. R. MAHADEVAN AND U. R.MAHADKAR . ........................................................... 941

Induction of Enzymes in Dormant Spores of Aspergillus oryzae. HYOGO SINOHARA.. 1070Detection of a f-Lactamase Markedly Active Against Carbenicillin in a Strain of

Pseudomonas aeruginosa. S. W. B. NEWSOM, R. B. SYKES, AND M. H. RICHMOND 1079Inducible -y-Butyrobetaine-Degrading Enzymes in Pseudomonas Species AK 1. G.

LINDSTEDT, S. LINDSTEDT, T. MIDTVEDT, AND M. TOFFT...................... 1094

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