noreen moin
TRANSCRIPT
Evaluation of resistance in wheat against Rhopalosiphum padi (L.) (Homoptera: Aphididae)
Noreen Moin
05-arid-345
PMAS-Arid Agriculture University Rawalpindi
CONTENTS
IntroductionMaterials and methods
Seedling bulk testAntibiosis testTolerance test
Results Seedling bulk testAntibiosis testTolerance test
Conclusion Recommendation
INTRODUCTION
Aphids are consider as serious pests of crops (Wheat, Barely, Maize etc)
Transmit virus (MMSV, BYDV)
Persist through out the wheat season
Reproduce Sexually
Asexually
INTRODUCTION continue…
Some important species of aphids in Pakistan
Bird cherry oat aphid (Rhopalosiphum padi (L))Wheat grain aphid (Sitobion avenae) Grain aphid (Sitobion miscanthi)
Corn leaf aphid (Rhopalosiphum maidis)
Control strategies Insect pest management
Benefits of IPMEnvironmentally sound method Economic
OBJECTIVES
Evaluation of resistant varieties of National Uniform Wheat Yeild Trials (NUWYT) Rain Fed (RF) against Bird cherry oat aphid by applying following tests
Seedling bulk test
Antibiosis test
Tolerance test
MATERIALS AND METHODS
Host was wheat 11 varieties of (NUWYT) Rain Fed (R.F) of year 2004-05.
Experiments were conducted under controlled environment
28+2 oC, 40-70% RH and photoperiod of 16:8h
Mass rearing was done in NARC by establishing the culture of Rhopalosiphum padi (L)
Resistance was evaluated by Seedling bulk test
Antibiosis test
Tolerance test
SEEDLING BULK TESTMetal trays measuring(51x35x9 cm)Eight rows of one cm depth25 seeds per genotype was sownTen aphids were released at seedling height of 5-6 cmDamage rating was recorded after 10-15 days
ANTIBIOSIS TEST
Two seeds were sown in 7 cm diameter potAt height of 5-6 cm, each variety was thinned to one One adult aphid was released Covered with plastic cage having two side ventilation holes, 6 cm diameter and 30 cm height One nymph was allowed to grow until it becomes sexually mature Nymphs reproduced daily were removed and counted for each seedling
TOELRANCE TEST
Seeds were sown as described in antibiosis testSeedlings were infested by aphids at the height of 5-6 cmTen adults were maintained
RESULTS Seedling bulk test
Varieties NUWYT (RF) No. of seedlings Damage rating (DR)
Nature of resistance
PR-87 21 4 MR
NR-241 17 4 MR
SN-128 20 5 MR
V-00BT004 16 5 MR
V-00055 18 6 MR
V-5 20 3 R
NRL-2017 22 7 S
NR-234 15 5 MR
RESULTS Seedling bulk test CONTI….
Varieties NUWYT (RF)
No. of seedlings Damage rating (DR) Nature of resistance
V-002467 15 4 MR
DN-44 18 4 MR
PR-83 25 5 MR
Chakwal-97 14 7 S
R = Resistant varieties with damage rating = 3MR = Moderately Resistant varieties with damage rating = 4-6S = Susceptible varieties with damage rating =7-9
RESULTSANTIBIOSIS TESTVarieties NUWYT (RF) Average no. of nymphs Remarks
PR-87 1.3 MF
NR-241 1.3 MF
SN-128 1.5 HF
V-00BT004 1.3 MF
V-00055 1.2 MF
V-5 1.0 MF
NRL-2017 0.9 MF
NR-234 1.1 MF
Varieties NUWYT (RF) Average no. of nymphs Remarks
V-002467 0.5 LF
DN-44 1.2 MF
PR-83 0.7 LF
Chakwal-97 1.6 HF
Wafaq-2001 1.3 MF
RESULTSANTIBIOSIS TEST CONTI..
LF = Least Fecund MF = Moderately Fecund HF = Highly Fecund
RESULTSTOLERANCE TESTVarieties NUWYT (RF) Average DR Remarks
PR-87 3.8 MT
NR-241 3.6 HT
SN-128 3.6 HT
V-00BT004 6.2 LT
V-00055 4.4 MT
V-5 3.6 HT
NRL-2017 4.4 MT
NR-234 5.4 MT
RESULTSTOLERANCE TEST CONTI..
Varieties NUWYT (RF) Average DR Remarks
V-002467 5.2 MT
DN-44 4.6 MT
PR-83 3.6 HT
Chakwal-97 4.2 MT
Wafaq-2001 4.6 MT
HT = Highly Tolerant MT = Moderately Tolerant LT = Least Tolerant
3.83.6 3.6
6.2
4.4
3.6
4.4
5.45.2
4.6
3.6
4.2
4.6
0
1
2
3
4
5
6
7
Wheat varieties NUWYT (RF) 2004-05 along with Wafaq 2001
Av
g D
am
ag
e R
ati
ng
PR-87
NR-241
SN-128
V-00BT004
V-00055
V-5
NRL-2017
NR-234
V-002467
DN-44
PR-83
Chakwal-97
Wafaq-2001
RECOMANDATIONS
The use of resistant varieties will remain the most logical and economical way of reducing damage of pests in wheat germplasm
CONCLUSIONS
In seedling bulk test V-5 was highly resistantIn antibiosis test least fecund line was PR-83In tolerance test four lines NR-241, SN-128, V-5 and PR-83 was found to be tolerant to aphid R.padi