avian tumor
DESCRIPTION
patologiTRANSCRIPT
AVIAN TUMOR
Prepared By
DEWI RATIH AGUNGPRIYONO, DVM, PhD
Division of PathologyDepartment of Veterinary Clinic, Reproduction and Pathologyp y , p gyFaculty of Veterinary Medicine, Bogor Agricultural University
D Diseases causing avian tumor :
1. Marek (MD)( )
2 Avian Leukosis (AL)2. Avian Leukosis (AL)
3 Reticul end theli sis (RE)3. Reticuloendotheliosis (RE)
ETIOLOGYMDV ALV REVMDV ALV REV
α Herpes virus Retrovirusα Retrovirusp RetrovirusSerotype 1 oncogenicSerotype 2 non-
oncogenic A i L k i Vi
Avian Sarcoma Virus
Sub grup A
oncogenicSerotype 3 HVT non-
oncogenic
Avian Leukosis Virus
Sub grup Boncogenic
Sub grup J
DNA Virus RNA Virus RNA VirusDNA Virus RNA Virus RNA Virus
TYPE OF TUMOR CELLSMD ALV REV
Lymphoma LymphomaSarcoma Lymphoma (mononuclear pleiomorphic cells :
LymphomaTumor of blood vessel, tumor of loose connective tissue
uniform lymphoblast p p
lymphocytes, lymphoblast, plasma
connective tissue, epithelial tumor
tumor cells, immature and mature
cell, macrophage) Lymphoma and Leukosis
uniform lymphoblast tumor cells, erythroblast erythroblast, myeloblast, myelocyte
TYPE OF TUMOR CELLSMD ALV REV
TRANSMISSIONMD ALV REV
Horizontal Horizontal, Vertical
Horizontal, Vertical Direct Contact Direct Contact
Indirect : Inhalation
Gross Pathology MD ALV REV
Nerve : enlarged Nerve : enlargedg
Skin : feather follicle Viscera : visceral tumor Viscera : visceral
tumor Viscera : visceral tumorSkin : feather follicle
tumorEye : greyy g y
8 – 10 weeks > 24 weeks > 24 weeks
Not all tumor cases could be observed grossly !
Gross Pathology gy
Grey Eye (right)lymphoid infiltration of iris that causes white discoloration. The pupil is often irregular (as shown here) and does not respond to ) pchanges of light. A normal eye is on the left.
Visceral Tumor (liver)Visceral Tumor (liver)
Gross Pathology
b /111/02patoipb U/111/02
Visceral Tumor(liver and spleen)( p )
Gross Pathology
Visceral Tumor (spleen)
patoipb U/30/01
Thickened proventricular wallGross Pathology
T i l (h ti)Tumor viseral (hati)patoipb U/60/01
Gross Pathology Gross Pathology
patoipb U/30/01
Visceral Tumor (lung)
Gross Pathology
Visceral Tumor (kidney)
Gross Pathology gy
Nerve Tumor (nervus Brachialis)
Gross Pathology
Nerve Tumor (nervus Ischiadicus)
Gross Pathology
The dorsal root ganglia from bird with Marek's disease
Gross Pathology
Skin Tumor / Feather Follicle Tumor
Gross Pathology gy
Skin Tumor / Feather Follicle Tumor
Gross Pathology gy
large subcutaneous lymphomas may be observed throughout the body (shown here in the leg on the left). A normal leg is shown on the right for comparison
Gross Pathology
os sternum tumor
Gross Pathology Gross Pathology
Costae Tumor
G P th l Gross Pathology
B F b i i TBursa Fabricious Tumor
Gross Pathology
Liver Hemangioma
Microscopic Lesion MD ALV REV
Nerve : infiltration Nerve : infiltration of pleiomorph lymphocyte, demyelinization,
mature and immature lymphoblast
Schwann cells proliferationB i i l Brain : perivascular accumulation lymphocyte pleiomorph lymphocyte pleiomorph, spread to neuropil, necrotizing malaciag
Histopathology p gy
N m lNormal
patoipb S/30/02Nerve Tumor
Histopathology p gy
Demyelinization
Histopatology p gy
tumor cells perivascular cuffing
Histopathology p gy
patoipb S/30/02Malacia
Histopathology MD ALV REV
Viscera : interstitial i filt ti f l i h
Visceral : interstitial i filt ti f
Visceral : interstitial i filt ti f infiltration of pleiomorph
lymphocytes tumor cellsinfiltration of lymphoblast tumor cells in many visceral
infiltration of lymphoblast tumor cells in many visceral
Skin : accumulation of pleiomorph lymphocyte
cells in many visceral organ
cells in many visceralorgan
pleiomorph lymphocyte at feather follicle epitheliumBone Marrow : multifocal accumulation l h t l i h
Bone Marrow : diffuse accumulation limfoblast tumor cellslymphocyte pleiomorph limfoblast tumor cells
Histopathology
Liver Tumor
Histopathology
interstitial ovary tumor cells
Histopathology
Feather Follicle Tumor
Histopathology MD ALV REV
Lymphoreticular : Lymphoreticular : lymphoid cells cytolisis → follicle depletion; Bursal interfollikular
Proliferation of lymphoblast tumor cells at spleen (red Bursal interfollikular
pleiomorph lymphocyte tumor cells infiltration
cells at spleen (red pulp) and bursa (intrafollicullar); tumor cells infiltration (intrafollicullar);
Vascular : Atherosclerosis
Vascular : Circulation of proliferated tumor cells (leukosis)cells (leukosis)
Histopathology
Interfollicular Bursa Fabricious Tumor, Lymphoid Follicle Atrophy
Histopathology
Interfollicular Bursa Fabricious Tumor
Intrafollicular Bursa Fabricious Tumor
Histopathology MD ALV REV
antigen Detection of antigen Detection of antigen Detection of gsurface
MATSA 5-40% MATSA negatif MATSA negatif
gsurface
gsurface
Ig M < 5 %Sel B 3-25%Sel T 60-90%
MATSA negatif Ig M 91-99 %Sel B 91-99%Sel T rare
MATSA negatif Ig M ?Sel B rareSel T plentySel T 60 90% Sel T rare Sel T plenty
MATSA : Marek’s Disease Associated Tumor Surface Antigen
Clinical Sign
MD ALV REVTransient Paralysis Sub clinic Sub clinicy
(Late clinical onset) (Late clinical onset)
patoipb U/116/02
ALV Infected Male
PreventionMD ALV REV
Vaccination Biosecurity BiosecurityBiosecurity
yBreeder control
y
(Culling positive parents)Breeder control
(Culling positive parents)
Non-specific Lesion
Egg Yolk Peritonitis
Non-Spesific Lesion
interstitial ovary tumor cells
Non-spesific Lesion
patoipb U/111/02
Necrotic Enteritis
patoipb U/111/02
Necrotic Enteritis
Non Spesific Lesion
T ll ithi i t ti l l i iTumor cells within intestinal lamina propria