AxION DSA System A Reference Notebook of DSA Applications—First Edition, 2012

MASS SPEC RESULTSIN SECONDS WITHDIRECT SAMPLE ANALYSIS

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REVOLUTIONIZE YOUR MASS SPECWORKFLOW Going from sample to results can often be complex

and time-consuming. That is why PerkinElmer has introduced a unique solution: the AxION® DSA (Direct

Sample Analysis) system. Nothing simplifies workflows more than AxION DSA. Diminish cumbersome method development, eliminate chromatography and dramatically reduce sample preparation.

Results in secondsThe innovative AxION DSA technology was developed for the rapid direct analysis of solid, liquid or gaseous samples. It offers a novel alternative to standard chromatographically interfaced solutions. Laboratories can now screen, compare and confirm virtually any sample, by introducing it directly to a mass spectrometer in a matter of seconds.

• No chromatography required.

• Avoid time consuming method development.

• Minimal sample preparation.

This reference notebook of direct sample analyses, highlights the performance characteristics of the AxION DSA. It features a diverse selection of applications, sample types and results across a broad array of industries.

The AxION DSA system will change the way you work by providing results-driven decisions in seconds.

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Index of Applications

• Atrazine in Water ................................................................................................................................................................14

• Carbamates in River Water ..................................................................................................................................................15

• Insecticide on Mosquito Nets ..............................................................................................................................................16

• Personal Care Products in River Water ................................................................................................................................. 17

environmental

• Limonene in Orange Peel ....................................................................................................................................................18

• Parabens in Lotions .............................................................................................................................................................19

chemical

Food and BeveraGe• Capsaicin and Dihydrocapsaicin in Peppers ...........................................................................................................................4

• Curcumins in Turmeric Powder ..............................................................................................................................................5

• Fungicide on Lemon Peel ......................................................................................................................................................6

• Fungicides on Oranges ..........................................................................................................................................................7

• Malathion on Peaches ...........................................................................................................................................................8

• Melatonin in Lazy Cake® .......................................................................................................................................................9

• Propanethiol S-oxide in Onions ...........................................................................................................................................10

• Resveratrol in Red Wine ...................................................................................................................................................... 11

• Sterols in Olive Oil ............................................................................................................................................................... 12

• Sweeteners in Diet Cola ...................................................................................................................................................... 13

• Components of Multivitamin Powder ..................................................................................................................................22

nUtraceUtical

ForenSicS• Date Rape Drug in Cola .......................................................................................................................................................20

• Testosterone in Steroid Powder ...........................................................................................................................................21

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TOF MS

Direct Sample analySiS

No sample preparation

Easy to use

Semi-quantitative

• Thesignalintensitiesprovidedtherelativeamountsofcapsaicinanddihydrocapsaicininthepeppers

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Capsaicinanddihydrocapsaicinarethecompoundsresponsibleforpepperhotness

• 1cm3piecesofcubanelle,chilli,andjalapeñopeppersweredirectlyanalyzed

Food and Beverage: Capsaicin and Dihydrocapsaicin in Peppers

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TOF MS

Direct Sample analySiS

No sample preparation

Easy to use

Complete characterization

• DSA/TOFanalysisofturmericpowderdetectsall3curcuminoidswithhighmassaccuracy

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Curcuminsaretheactiveingredientinturmericpowderusedforfoodflavoring,dyeing,andpossiblemedicalbenefits

• Turmericpowderwasdirectlyanalyzedinaglasscapillary

Food and Beverage: Curcumins in Turmeric Powder

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TOF MS

Direct Sample analySiS

Outstanding sensitivity

Fast accurate mass

Assures food safety

• Residualimazalilwasconfirmedwithhighmassaccuracy

• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration

• Pesticidessuchasimazalilareoftenusedtoprotectfruitsandvegetablesfrominsects

• Awashed1cm3pieceoflemonpeelwasextractedwithmethanolandanalyzed

Food and Beverage: Fungicide on Lemon Peel

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TOF MS

Direct Sample analySiS

No sample preparation

Easy to use

Assures food safety

• DSA/TOFanalysisdetectedthepresenceoftwofungicidesontheorangeskin

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Tracesoffungicidesonourfoodposeapotentialrisktohumanhealth

• Aswabbingwithmethanolfromanorangeboughtatagrocerystorewasanalyzed

Food and Beverage: Fungicides on Oranges

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TOF MS

Direct Sample analySiS

Easy to use

Low detection limits

Fast, accurate mass

• DSA/TOFanalysisoftheswabsampleconfirmedmalathion,withhighmassaccuracy,at800timeslowerthantheEPAtolerancelevel

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Malathionisacommonlyusedinsecticide.TheEPAtolerancelevelformalathiononpeachesis8000ng/g

• Apeachsurfacewasspikedwith10ng/gmalathion,thenswabbedwithmethanolandanalyzed

Food and Beverage: Malathion on Peaches

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TOF MS

Direct Sample analySiS

No sample preparation

Confirms active ingredient

Rapid analysis

• Directanalysisconfirmstheactiveingredientmelatoninwithhighmassaccuracy

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• LazyCakes®,marketedas“relaxationbrownies”arelacedwiththesleepaidmelatonin

• Thepackagingiskid-friendlyandchildrenhaveeatenthemandfallenasleep

• Apieceofcakewasanalyzedtodeterminetheingredientthatwascausingsleepiness

Food and Beverage: Melatonin in Lazy Cake®

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TOF MS

Direct Sample analySiS

No sample preparation

Easy to use

Fast, accurate mass

• Withnosamplepreparation,DSA/TOFanalysisoftheonionsampledetectedPropanethiolS-oxidewithhighmassaccuracy

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• PropanethiolS-oxideinonionsisknowntocausetheeye’stotear(whencuttingtheonion)

• 1cm3pieceofonionwasdirectlyanalyzed

Food and Beverage: Propanethiol S-oxide in Onions

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TOF MS

Direct Sample analySiS

Easy to use

Increased sensitivity

Rapid analysis

• DSA/TOFanalysisinTrapPulse™modeof1uLofredwine(onamesh)providestherequiredsensitivityfordetectionandconfirmationofresveratrol

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Resveratrolisnaturallyoccurringpolyphenolpresentingrapeskinandseeds

• Itisanantioxidantthoughttobepresentinhigherlevelsinredwineandlinkedtomanyhealthbenefits

Food and Beverage: Resveratrol in Red Wine

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TOF MS

Direct Sample analySiS

Easy to use

Comparison of products

Rapid analysis

• Oliveoilscanrapidlybecomparedfordetectionofbeneficialsterols

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Somesterolsinoliveoilarethoughttodecreasebloodcholesterol

• Saponifiedoliveoilwasdirectlyanalyzedforsterols

• DSA/TOFanalysisdetectsthebeneficialsterolsinoliveoilwithminimalsamplepreparation

Food and Beverage: Sterols in Olive Oil

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TOF MS

Direct Sample analySiS

Easy to use

Component analysis

No sample preparation• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Artificialsweetenersareoftenusedindietcola

• Sweetenersindietcolawasdirectlyanalyzedwithoutanysamplepreparation

• DSA/TOFanalysisconfirmssweetenersusedindietcolaaswellasotheradditiveswithaccuratemass

Food and Beverage: Sweeteners in Diet Cola

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TOF MS

Direct Sample analySiS

Environmental: Atrazine in Water

Results in seconds

Increased sensitivity

Confirms water safety

• DSA/TOFanalysisof2uLofwateronameshconfirmspresenceofatrazinewithamassaccuracy<1ppm

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• UseofTrapPulse™modeincreasessensitivity8foldandgivesgreatermassaccuracy

• Thepesticideatrazineisacontaminantofsurfaceanddrinkingwater

• Themaximumlevelofatrazineinwaterhasbeensetas0.003mg/L

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TOF MS

Direct Sample analySiS

Environmental: Carbamates in River Water

No complex method development

Fast, accurate mass

Ensures water quality

• DSA/TOFanalysisconfirmspresenceofcarbamates,withhighmassaccuracy

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• 10ppbspikedriversamplessimplyfilteredandpipettedontoamesh

• Carbamatesareknowncontaminantsofriverwater,EPAmaximumconcentrationof10ug/L

• ExistingprotocolsrequirelengthySPEworkupfollowedbyLC,LC/MSorGC/MSanalysis

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TOF MS

Direct Sample analySiS

Environmental: Insecticide on Mosquito Nets

Results in seconds

Assures product authenticity

Protects consumers

• DSA/TOFanalysisofanetsectionconfirmspresenceofdeltamethrinattherequiredleveltobeeffective

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• ExistingprotocolsrequireextractionfollowedbyHPLCorGC/MSanalysis

• DeltamethrinisaWHOapprovedinsecticidefortreatmentofmosquitonets

• Treatednetscommandahigherpricebutsomesuppliersomittheinsecticide

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TOF MS

Direct Sample analySiS

No complex method development

Comprehensive analysis

Ensures water quality

• 10ppbspikedriversamplessimplyfiltered,10mMammoniumformateadded,andpipettedontoamesh

• DSA/TOFanalysisconfirmspresenceofpersonalcareproductsandpesticides,withhighmassaccuracy

• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration

• Personalcareproductsposearisingconcernasacontaminantinpostwatertreatmentriverwaters

• ExistingprotocolsrequirelengthySPEworkupfollowedbyLC,LC/MSorGC/MSanalysis

Environmental: Personal Care Products in River Water

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TOF MS

Direct Sample analySiS

Easy to use

Compound confirmation

No sample preparation

• Limoneneismeasuredinorangepeelwithdirectanalysisandaccuratemassconfirmation

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Limoneneisamoleculethatgivesorangestheirstrongsmellandisoftenusedincleaningproductsandaprecursorforchemicalsynthesis

• Samplepreparationandmethoddevelopmentforanalysisoflimoneneislengthyandtimeconsuming

Chemical: Limonene in Orange Peel

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TOF MS

Direct Sample analySiS

Easy to use

Complete characterization

No sample preparation

• DSA/TOFdirectanalysisoflotionconfirmsparabensinlotionwithaccuratemass

• Theanalysiswasperformedin15secondswithminimalsamplepreparationandexternalcalibration

• Parabensareaclassofchemicalsusedinpreservationofcosmetics

• Samplepreparationandmethoddevelopmentofparabensislengthyandtimeconsuming

Chemical: Parabens in Lotions

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TOF MS

Direct Sample analySiS

No sample preparation

Low detection limits

Fast, accurate mass

• Withnosamplepreparation,DSA/TOFanalysisofthecolasampledetectedthedrugwithhighmassaccuracy

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Theminimumlevelofthedaterapedrug,Gamma-HydroxyButyricAcid,whichaffectshumansis1mg/mL

• Acolasamplewasspikedwiththedrugat0.1mg/mL,10-foldlessthantheminimumdetectionlimit

Forensics: Date Rape Drug in Cola

21www.perkinelmer.com/DSA

TOF MS

Direct Sample analySiS

Easy to use

Confirms compound identity

Fast, accurate mass

• Theanalysiswasperformedin15secondswithnosamplepreparationandexternalcalibration

• Testosteroneisananabolicsteroidthatcanbeusedtoenhancephysicalperformanceinathletes

• Testosteronepowderwasdirectlyanalyzedinaglasscapillary

• Confirmationoftestosteronewasachievedinsecondswithhighmassaccuracy

Forensics: Testosterone in Steroid Powder

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TOF MS

Direct Sample analySiS

No sample preparation

Complete component analysis

Confirms product authenticity

• DSA/TOFanalysisofEmergen-C®powderinaglasscapillarydetectscomponentswithahighmassaccuracy

• Emergen-C®isadietarysupplementdesignedtosupporthealthymetabolicfunction

• Analysisinbothpositiveandnegativemodesensurescompleteproductcharacterization

Nutraceuticals: Components of Multivitamin Powder

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