BACTERIAL CAUSES OF FIN BACTERIAL CAUSES OF FIN ROT IN SOME FRESH WATER ROT IN SOME FRESH WATER

FISHES.FISHES.EnanyEnany, M. E., El , M. E., El SayedSayed, M. E., , M. E., DiabDiab. A. S.,. A. S.,

HassanHassan, S. M., and El , S. M., and El –– GamalGamal, R.M.**, R.M.***Department of Microbiology, Faculty of Vet. Medicine, *Department of Microbiology, Faculty of Vet. Medicine,

Suez Canal University.Suez Canal University.** Central Lab. For aquaculture, ** Central Lab. For aquaculture, AgrAgr. Research Center; . Research Center;

AbbessaAbbessa

170 naturally infected fishes (90 tilapia spp.,

50 Clarias lazeraand 30 Commen carp)

with fin rotrevealed clinically progressive erosions,

congestion and hemorrhages of the body finsespecially the caudal and dorsal fins with edema

and sloughing in some cases .

naturally infected fishesrevealed the presence of 468 bacterial isolates

related to 8 bacterial genera and species .A. hydrophila (198)P. fluorescens (102)

Streptococcus sp.(36)F. columnaris (36)Klebsiella sp. (48)

E. coli (24)Proteus sp(12)

And Shigٍٍella sp. (12)

Table (1): Collective data of bacterial isolates from examined naturally infected fish species.

34.434.44684681212(2.6)(2.6)

1212(2.6)(2.6)

2424(5.1)(5.1)

3636(7.7)(7.7)

4848((10.310.3))

3636(7.7)(7.7)

102 102 (21.8)(21.8)

198 (42.3)198 (42.3)170170TotalTotal

6.36.3868611(1.16%)(1.16%)

11(1.16%)(1.16%)

88(9.30%)(9.30%)

99(10.47%)(10.47%)

99((10.47%10.47%))

6 6 (6.98%)(6.98%)

18 18 (20.93%)(20.93%)

34 34 (39.53%)(39.53%)

3030CarpCarp

10.010.013613644(2.94%)(2.94%)

33(2.21%)(2.21%)

66(4.41%)(4.41%)

99(6.62%)(6.62%)

1414((10.29%10.29%))

11 11 (8.09%)(8.09%)

30 30 (22.06%)(22.06%)

59 59 (43.38%)(43.38%)

5050ClariasClarias

18.118.124624677(2.85%)(2.85%)

88(3.25%)(3.25%)

1010(4.06%)(4.06%)

1818(7.32%))(7.32%))

2525((10.16%10.16%))

19 19 (7.73%)(7.73%)

54 54 (21.95%)(21.95%)

105 105 (42.68%)(42.68%)

9090TilapiaTilapia

ShegellaShegellaProteus Proteus speciesspecies

E. Coli E. Coli StreptocoStreptococc speciesspecies

KlebsiellaKlebsiella speciesspecies

FlecibacteFlecibacterr speciesspecies

PseudomoPseudomonasnas

species species

AeromonAeromonasas speciesspecies

%%TotalTotalNo.No.

No. of No. of infected infected

fishes with fishes with fin rotfin rot

Fish Fish speciesspecies

N. B.: % was calculated according to total number of isolates of each species.Total No. was calculated according to the total number of the samples.

Table (2): Distribution of Bacterial isolates among various tissues and organs of naturally infected fish species with tail and fin rot.

----------------50.0050.003316.6716.6711----33.3333.332266CarpCarp

----------------18.1818.182245.4545.4555----36.3636.36441111ClariasClarias

----------------36.8436.847731.5831.5866----31.5831.58661919TilapisTilapisF.F.columnariscolumnaris

----16.6716.673311.1111.1122----5.565.561127.7827.785516.6716.673322.2222.22441818CarpCarp

----6.676.67223.333.3311----6.676.67223.003.009920.0020.006633.3333.3310103030ClariasClarias

----12.9612.96775.565.5633----5.565.563329.6329.63161616.6716.679929.6329.6316165454TilapisTilapisPsPs..fluorscencefluorscence

ss

----2.942.941114.7114.71555.885.882220.5920.597711.7611.764414.7114.715529.4129.4110103434CarpCarp

3.393.39226.776.774410.1710.17666.776.774418.6418.6411118.468.465520.3920.39121225.4225.4215155959ClariasClarias

3.813.81446.676.677712.3712.3713135.705.706617.1417.1418188.598.599918.118.1191937.6237.622929105105TilapisTilapisA. A. hydrophilahydrophila

%%No.No.%%No.No.%%NoNo..

%%NNoo..

%%No.No.%%NoNo..

%%No.No.%%No.No.isolateisolatess

SpeciesSpecies

Ascitic fluidAscitic fluidOvariesOvariesMuscleMuscleSpleenSpleenGillsGillsKidneyKidneyLiverLiverFin & tail Fin & tail lesionslesions

TotalTotalFishFishIsolatesIsolates

!!Fig. (1) Tilapia Fig. (1) Tilapia niloticanilotica showing progressive erosions showing progressive erosions of body fins, especially caudal and ventral fin, focal to of body fins, especially caudal and ventral fin, focal to diffuse necrosis of muscle and detachment of scales.diffuse necrosis of muscle and detachment of scales.

!!Fig. (2) Tilapia Fig. (2) Tilapia niloticanilotica showing erosions of body showing erosions of body fins, detachment of scales and skin congestion.fins, detachment of scales and skin congestion.

!!Fig.(3) Fig.(3) ClariasClarias lazeralazera showing erosions and showing erosions and congestion of the body fins.congestion of the body fins.

The postmortem changes of naturally infected fishes

wereabdominal ascitis,

enlargement and congestion of the liver, kidneys, spleen and intestine

with distension and congestion of the gall bladder.

!!Fig.(4) Common carp showing congestion of Fig.(4) Common carp showing congestion of internal organs specially liver as well as inflamed internal organs specially liver as well as inflamed

musclemuscle

!!Fig. (5)Common carp showing detachment of Fig. (5)Common carp showing detachment of scales, erosion of caudal fin, congestion of internal scales, erosion of caudal fin, congestion of internal

organs & bloody ascitic fluid.organs & bloody ascitic fluid.

!!Fig. (6) Muscles showing extensive necrosis and Fig. (6) Muscles showing extensive necrosis and focal replacement to the necrotic muscles by edema, focal replacement to the necrotic muscles by edema,

hemorrhage, and mononuclear leukocyteshemorrhage, and mononuclear leukocytes

!!Fig. (7) Gills showing edema, congestion, and hemorrhage Fig. (7) Gills showing edema, congestion, and hemorrhage in the gill arch, necrosis and desquamation in the gill in the gill arch, necrosis and desquamation in the gill

lamellae along with mononuclear lamellae along with mononuclear leukocyticleukocytic infiltrationinfiltration

!!Fig. (8) Kidney showing wide spread necrosis of Fig. (8) Kidney showing wide spread necrosis of the renal tubules along with interstitial edema, the renal tubules along with interstitial edema,

hemorrhage and mononuclear leukocyteshemorrhage and mononuclear leukocytes

!!Fig. (9) Liver showing extensive vacuolar Fig. (9) Liver showing extensive vacuolar degeneration and focal areas of degeneration and focal areas of coagulativecoagulative

necrosisnecrosis

The pathogencity of isolated strains revealed that

A.hydrophila appeared to be highly virulent ( 87-100%) mortality in injected groups

followed by P .fluorescens (50%)and F .columnaris (37.5%)

Table (3):Route of infection and pattern of mortality in armout catfish experimentally inoculated with fish pathogenic isolated bacteria.

--------I/MI/MCytophagaCytophaga88IXIX

--------I/PI/PSterile brothSterile broth88VIIIVIII

87.587.57711--11------111111--22--------I/MI/MA. A. ydrophilaydrophila &&P.fluorescensP.fluorescens & &

F.columnarisF.columnaris

88VIIVII

252522--11----------11--------------I/PI/PP.fluorescensP.fluorescens&&

F.columnarisF.columnaris

88VIVI

10010088--11--11----11222211----------I/MI/MA. A. ydrophilaydrophila & & F.columnarisF.columnaris

88VV

87.587.577----------11--1111221111------I/PI/PA. A. ydrophilaydrophila &&P.fluorescensP.fluorescens

88IVIV

37.537.533--11------11------------11----I/MI/MF. F. olumnarisolumnaris88IIIIII

50504411--1111------11--------------I/PI/PP.fluorescensP.fluorescens88IIII

10010088--11--11--11------2233--------I/PI/PA. A. ydrophilaydrophila88II

%%No.No.

TotalTotal151514141313121211111010998877665544332211Route of Route of inf.inf.

Infected organismInfected organismNo. fishNo. fishFish Fish groupgroup

8 fish inoculated

Sensitivity test of isolated strains showed that

kanamycin and nalidexic acid were the drugs of choice used for control and

treatment of fin rot disease.

Table(4): Antibiotics sensitivity of Bacteria isolated from naturally infected fish

+2+2+4+4--+1+1--+4+4+4+4----------Carp Carp ((6 isolates6 isolates((

+1+1+4+4--+1+1+1+1+4+4+3+3--+1+1--+1+1--Claris Claris ((11 isolates11 isolates((

+2+2+4+4--+1+1--+4+4+4+4----+1+1--+1+1Tilapia Tilapia ((19 isolates19 isolates((columnariscolumnaris. . FF

+2+2+3+3+2+2+2+2+3+3+3+3+2+2--+1+1+2+2+2+2+1+1Carp Carp ((18 isolates18 isolates((

+3+3+2+2+1+1+3+3+4+4+3+3+1+1+1+1+1+1+2+2+2+2+2+2Claris Claris ((30 isolates30 isolates((

+3+3+2+2+2+2+3+3+4+4+4+4+2+2--+2+2+2+2+2+2--Tilapia Tilapia ((54 isolates54 isolates((fluorscencesfluorscences. . PsPs

+1+1+3+3----+4+4+4+4+4+4+1+1+2+2+1+1+2+2+2+2Carp Carp ((34 isolates34 isolates((

+3+3+3+3--+3+3+3+3+4+4+3+3+1+1+1+1--+2+2+1+1Claris Claris ((59 isolates59 isolates((

+2+2+3+3--+1+1+3+3_+4_+4+4+4+1+1+3+3+3+3+2+2+2+2Tilapia Tilapia ((105 isolates105 isolates((AA. . hydrophilahydrophila

SXTSXTTETEGGSSNNNANAKKEEDDCLCLCCAMAMXX

AntibioticsAntibioticsFishFish

SpeciesSpecies

IsolatesIsolates

AMX= Amoxicillin E= Erythromycin G= SulfonamidesC= Chloramphincol K= Kanamycin NA= Nalidixic acidCL= Colistin N= Neomycin SXT= Trimethoprim sulfonamidesD= Doxycycline S= Streptomycin Te= Tetracycline

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