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Bioinformatics Lab Non-duplicate Unigenes Selection Protocol Large-scale EST assembly using phrap Contigs Unassembled ESTs Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs Rearray selected ESTs to 384-well plates Plasmid DNA preps 5’ sequencing 3’ sequencing PCR amplification of inserts Unigenes Sequence processing EST validation and duplicates removal Probe delivery Bioinformatics Lab

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Non-duplicate Unigenes Selection Protocol. Large-scale EST assembly using phrap. Contigs. Unassembled ESTs. Unigenes. Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs. Rearray selected ESTs to 384-well plates. - PowerPoint PPT Presentation

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Page 1: Bioinformatics

Bioinformatics

Lab

Non-duplicate Unigenes Selection Protocol

Large-scale EST assembly using phrap

Contigs Unassembled ESTs

Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs

Rearray selected ESTs to 384-well plates

Plasmid DNA preps

5’ sequencing

3’ sequencing PCR amplification of inserts

Unigenes

Sequence processing

EST validation and duplicates removal

Probe delivery

Bioinformatics

Lab

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