Bioinformatics

Lab

Non-duplicate Unigenes Selection Protocol

Large-scale EST assembly using phrap

Contigs Unassembled ESTs

Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs

Rearray selected ESTs to 384-well plates

Plasmid DNA preps

5’ sequencing

3’ sequencing PCR amplification of inserts

Unigenes

Sequence processing

EST validation and duplicates removal

Probe delivery

Bioinformatics

Lab