bioknowledgy presentation on a.5 neuropharmacology
TRANSCRIPT
Essential idea: Communication between neurons can be altered through the manipulation of the release and reception of chemical messengers.
A.5 Neuropharmacology
The synaptic cleft between neurons (shown in the image) are the points at which a neural pathways can be influenced by neurotransmitters and other molecules, both natural and artificial.
By Chris Paine
http://www.bioknowledgy.info/ https://i.ytimg.com/vi/mItV4rC57kM/maxresdefault.jpg
Understandings, Applications and SkillsStatement Guidance
A.5.U1 Some neurotransmitters excite nerve impulses in postsynaptic neurons and others inhibit them.
A.5.U2 Nerve impulses are initiated or inhibited in post-synaptic neurons as a result of summation of all excitatory and inhibitory neurotransmitters received from presynaptic neurons.
A.5.U3 Many different slow-acting neurotransmitters modulate fast synaptic transmission in the brain.
A.5.U4 Memory and learning involve changes in neurones caused by slow-acting neurotransmitters.
A.5.U5 Psychoactive drugs affect the brain by either increasing or decreasing postsynaptic transmission.
A.5.U6 Anesthetics act by interfering with neural transmission between areas of sensory perception and the CNS.
A.5.U7 Stimulant drugs mimic the stimulation provided by the sympathetic nervous system.
A.5.U8 Addiction can be affected by genetic predisposition, social environment and dopamine secretion.
A.5.A1 Effects on the nervous system of two stimulants and two sedatives.
Examples of stimulants are nicotine, cocaine or amphetamines. Examples of sedatives are benzodiazepines, alcohol or tetrahydrocannabinol (THC).
A.5.A2 The effect of anesthetics on awareness.A.5.A3 Endorphins can act as painkillers.A.5.S1 Evaluation of data showing the impact of MDMA
(ecstasy) on serotonin and dopamine metabolism in the brain.
http://outreach.mcb.harvard.edu/animations/synaptic.swf
Review: 6.5.U7 Synapses are junctions between neurons and between neurons and receptor or effector cells.
Review: 6.5.U8 When presynaptic neurons are depolarized they release a neurotransmitter into the synapse. AND 6.5.U9 A nerve impulse is only initiated if the threshold potential is reached.
Action potential is the reversal (depolarization) and restoration (repolarization) of the membrane potential as an impulse travels along it.
Review: 6.5.U4 An action potential consists of depolarization and repolarization of the neuron.
The depolarisation of the membrane potential causes the voltage gated Na+ channels to close and the voltage gated K+ channels open. K+ diffuses out of the neuron rapidly and the membrane potential becomes negative again (repolarisation)
3
http://www.ib.bioninja.com.au/_Media/action_potential_med.jpeg
4 Before the neuron is ready to propagate another impulse the distribution of Na+ (out) and K+ (in) needs to be reset by the Na+/K+ pump, returning the neuron to resting potential. This enforced rest (refractory period) ensures impulses can only travel in a single direction.
The sodium-potassium pump (Na+/K+ pump) maintains the electrochemical gradient of the resting potential. Some K+ leaks out of the neuron (making the membrane potential negative, -70mv).
1
In response to a stimulus (e.g. change in membrane potential) in an adjacent section of the neuron some voltage gated Na+ channels open and sodium enters the neuron by diffusion. If a sufficient change in membrane potential is achieved (threshold potential) all the voltage gated Na+ channels open. The entry of Na+ causes the membrane potential to become positive (depolarisation)
2
Review: 6.5.A1 Secretion and reabsorption of acetylcholine by neurons at synapses.
http://faculty.pasadena.edu/dkwon/chap%208_files/images/image61.png
Acetylcholine is a neurotransmitter used in many synapses through the nervous system
One use is at the neuromuscular junction, i.e. it is the molecule that motor neurons release to activate muscles. Interfering with the action of acetylcholine can cause a range of effect from paralysis to convulsions.
A.5.U1 Some neurotransmitters excite nerve impulses in postsynaptic neurons and others inhibit them.
Excitatory and inhibitory neurotransmitters Acetylcholine (ACh) is an example of an excitatory neurotransmitter.
It causes an influx of positive sodium ions (Na+) into the post-synaptic neuron making the membrane potential more positive until the threshold is reached (-50mv) at which point the post-synaptic neuron starts to depolarise.
-70mv
time
threshold
resting potential
depolarisation
hyperpolarisation
-50mv
Gamma aminobutyric acid (GABA) is the main inhibitory neurotransmitter in mammals.
It hyperpolarizes post-synaptic neuron by causing an influx of negative chlorine ions (Cl-) making the neuron’s membrane potential more negative. A release of GABA means that it is harder for ACh to inact depolarisation in the post-synaptic neuron. It either takes longer for the threshold to be reached or the action potential is inhibited and does not occur.
A.5.U2 Nerve impulses are initiated or inhibited in post-synaptic neurons as a result of summation of all excitatory and inhibitory neurotransmitters received from presynaptic neurons.
Summation of post-synaptic potential (PSP)When a post-synaptic neuron receives excitatory and/or inhibitory potentials it 'adds' the potentials together. If the summation makes the membrane potential sufficiently positive to reach the threshold then depolarisation is occurs and an action potential is generated in the post-synaptic neuron.
http://www.old-ib.bioninja.com.au/_Media/summation_med.jpeg
Spatial summation is where the addition of PSPs occurs across a number of synapses
Temporal summation is when two or more PSPs arrive from the same synapse in rapid succession
Summation involves the addition of both Excitatory (EPSP) and inhibitory (IPSP) potentials
A.5.U3 Many different slow-acting neurotransmitters modulate fast synaptic transmission in the brain.
Slow-acting neurotransmittersACh and GABA are examples of fast acting neurotransmitters. Slow-acting neurotransmitters (such as Epinephrine, Norepinephrine, Dopamine and Serotonin) have a different, slower mode of action, which can modulate the effect of fast-acting neurotransmitters.
Fast-acting neurotransmitters Slow-acting neurotransmitters
Milliseconds Hundreds of milliseconds
Specific - Act across a single synapse Localised to an area – can diffuse to affect a number of neurons
Bind to post-synaptic receptors
Cause an influx of ions Cause the release of secondary messengers within the neuron
Short-lived effect - the neurotransmitter is usually broken down rapidly
Longer-lived effect – secondary messengers can still be working several days later
Examples: ACh, GABA Examples: Epinephrine, Norepinephrine, Dopamine and Serotonin
A.5.U4 Memory and learning involve changes in neurons caused by slow-acting neurotransmitters.
Improving our understanding of slow-acting neurotransmitters therefore can lead to therapeutic applications in related conditions, including
depression, Parkinson's disease, epilepsy and neuropathic pain.
Slow-acting neurotransmitters – memory and learningLong-term potentiation (LTP) is the persistent strengthening of synapses based on recent patterns of neural activity.
LTP associated with slow-acting neurotransmitters increasing synaptic transmission in the affected neurons
LTP is widely considered one of the major cellular mechanisms that underlies learning and memory.
The strengthening of synapses caused by LTP is an example of neural plasticity.
n.b. It is dangerous to assume that without LTP and neural plasticity that no learning or memory can occur; these are highly complex processes influenced by a range of factors.
https://commons.wikimedia.org/wiki/File:Epinephrine.svg
Epinephrine
Dopamine
https://commons.wikimedia.org/wiki/File:Dopamin_-_Dopamine.svg
A.5.A3 Endorphins can act as painkillers.
Endorphins – natural painkillers
https://commons.wikimedia.org/wiki/File:Alpha-endorphin.svg
Pain receptors are specialised cells that transmit impulses along neural pathways to the CNS when damage is done to the body, e.g. a cut in the skin.
Endorphins (type of oligopeptide) are secreted by the pituitary gland. They act upon the synapses of the neural pathways involved in the perception of pain.
Endorphins bind to opioid receptors in the synapse inhibiting synaptic transmission and hence lessening or preventing the perception of pain.
A.5.U5 Psychoactive drugs affect the brain by either increasing or decreasing postsynaptic transmission.
Learn more using the animation: http://www.jellinek.eu/brain/index.html?KeepThis=true&TB_iframe=true&height=588&width=672
How do psychoactive drugs affect the brain?Before thinking about how drugs affect the synapses, be sure you understand how they work and are reset. • Some NTs have a normal excitatory function• Other NTs have a normal inhibitory function
In general, psychoactive drugs can: 1. Increase or decrease the release of NTs (e.g.
THC – cannabis)2. Breakdown re-uptake proteins which are
responsible for returned used components of NTs to the pre-synaptic neuron (ready to use again)
3. Block re-uptake proteins (e.g.cocaine) so the NTs remain in the synapse
4. Mimic or block NTs, binding to the receptors on post-synaptic membranes
5. Inhibit production of new NTs
A.5.U5 Psychoactive drugs affect the brain by either increasing or decreasing postsynaptic transmission.
e.g. alcohol enhances GABA’s effects
The sympathetic nervous
system either prepares for action or
promotes activity. Common changes
that it causes are to heighten
awareness, increase energy levels, self-
confidence, heart rate, blood pressure
and body temperature. Stimulant drugs have similar
effects upon the body. Mild stimulants
can be found in food, for example
caffeine in tea and coffee, and
theobromine in chocolate. Stronger
stimulants, for example amphetamines,
can be used in medicine to treat
conditions such as depression or
Attention-Deficit/Hyperactivity Disorder
(AD/HD). Stimulants, such as
amphetamines and cocaine, are also
misued illegally used for recreation.
A.5.U7 Stimulant drugs mimic the stimulation provided by the sympathetic nervous system.
A.5.A1 Effects on the nervous system of two stimulants and two sedatives.
Stimulant drugs increase post-synaptic transmission
Examples: • Nicotine, amphetamines, cocaine
What is the effect of cocaine? Without cocaine: • Dopamine is re-uptaken by pumps on the pre-synaptic
membrane.
With Cocaine:• Cocaine blocks re-uptake pumps• Dopamine remains in synaptic cleft• More dopamine continues to be released• Summative increase in post-synaptic transmission
Effects on mood: • Dopamine is involved in reward pathways and causes
enhanced feelings of pleasure, which last longer than normal.
Effects on behaviour:• feelings of euphoria• increased energy and alertness• highly addictive• association with depression as body reduces production
of own dopamine over timehttp://is.gd/Jellinek
A.5.A1 Effects on the nervous system of two stimulants and two sedatives.
Stimulant drugs increase post-synaptic transmission
Examples: • Nicotine, amphetamines, cocaine
What is the effect of nicotine? Without cocaine: • Dopamine is re-uptaken by pumps on the pre-synaptic
membrane.
With nicotine:• Nicotine mimics the action of ACh; it binds to Ach
receptors triggering action potential in the post-synpatic neuron
• Unlike ACh Nicotine is not easily broken down and remains in synaptic cleft and continues to stimulate the post-synpatic neuron
Effects on mood: • Ach causes the release of dopamine, which in turn
causes enhanced feelings of pleasure
Effects on behaviour:• feelings of euphoria• increased calmness and alertness• Addictive - as the brain develops a tolerance for nicotine
is becomes needed for normal functioninghttp://is.gd/Jellinek
A.5.A1 Effects on the nervous system of two stimulants and two sedatives.
http://is.gd/Jellinek
Sedative drugs decrease post-synaptic transmission
Examples: • Alcohol, benzodiazapines, THC
What is the effect of tetrahydrocannibol (THC)? Without THC: • Dopamine release is moderated (inhibited) by GABA
With THC:• THC mimics anandamide cannabinoids and inhibits
GABA release by binding to cannabinoid receptors• GABA cannot inhibit dopamine release therefore
more dopamine is released
Effects on mood: • Dopamine is involved in reward pathways and
causes enhanced feelings of pleasure
Effects on behaviour:• intoxication• hunger• memory impairment• potential dependency
A.5.A1 Effects on the nervous system of two stimulants and two sedatives.
http://is.gd/Jellinek
Sedative drugs decrease post-synaptic transmission
Examples: • Alcohol, benzodiazapines, THC
What is the effect of Alcohol? Without Alcohol: • GABA inhibits the post-synaptic transmission• Glutamate is an excitatory NT that binds to receptors
increasing post-synaptic transmission
With Alcohol:• Alcohol increases the inhibitory effect of GABA by causing
GABA to remain bound to it’s receptor for longer • Alcohol binds to glutamate receptors preventing glutamate
from stimulating the the post-synaptic neuron.
Effects on mood: • Alcohol indirectly causes a dopamine release that in turn
causes enhanced feelings of pleasure
Effects on behaviour:• Calm relaxed feeling• memory impairment• Increases (worsens) reaction speeds• Affects breathing, body temperature regulation and
appetite• potential dependency
A.5.U6 Anesthetics act by interfering with neural transmission between areas of sensory perception and the CNS. AND A.5.A2 The effect of anesthetics on awareness.
Anaesthetics Anaesthetics cause a temporary loss of sensation, there are two main types of anaesthetic:• Local - cause numbness (a loss of sensation) in an area of the
body, e.g. no feeling of the gums during a dental procedure• General - cause unconsciousness and therefore a lack of
sensation throughout the body
https://commons.wikimedia.org/wiki/File:Preoxygenation_before_anesthetic_induction.jpg
Many anaesthetics have multiple roles and hence affect the body in different ways, due to this and the incomplete understanding we have of them they are usually administered by specialised medical practitioners called Anaesthetists.
Anaesthetics are very varied group of molecules that affect neural pathways in different ways. How different anaesthetics work is not fully understood, but it is known that many do affect the receptors found in synapses.
General anaesthetics block ‘long-distance communication’ hence affecting (often inhibiting) the transmission of impulses from receptors (areas of sensory perception) and the CNS.
A scale of harm for drugsDraw markers on the graph for each drug to indicate how much physical harm and dependence (addiction) you think it causes:• Nicotine• Cocaine• Amphetamines (speed)• Alcohol• Canabis (THC)• Ecstasy (MDMA)
Discuss:• How can drugs cause
physical harm? • How can drug use lead to
addiction (dependency)? • What factors contribute to
development of addiction?
https://commons.wikimedia.org/wiki/File:Rational_scale_to_assess_the_harm_of_drugs_(mean_physical_harm_and_mean_dependence). svg
A scale of harm for drugs
https://commons.wikimedia.org/wiki/File:Rational_scale_to_assess_the_harm_of_drugs_(mean_physical_harm_and_mean_dependence). svg
Draw dots on the graph for each drugs to indicate how much physical harm and dependence (addiction) you think they cause:• Nicotine• Cocaine• Amphetamines (speed)• Alcohol• Canabis (THC)• Ecstasy (MDMA)
Discuss:• How can drugs cause
physical harm? • How can drug use lead to
addiction (dependency)? • What factors contribute to
development of addiction?
A.5.U8 Addiction can be affected by genetic predisposition, social environment and dopamine secretion.
Addiction
Breaking an addiction is very difficult and can cause severe physical and mental reactions (withdrawal symptoms).
Genetic predisposition• Many genes with roles in addiction have been identified
(with the help of animal models, especially mice)• For example the A1 allele of the dopamine receptor gene
DRD2 is more common in people addicted to alcohol or cocaine.
dependence on a substance or activity resulting in its repeated and compulsive use
Causes of Drug
Addiction
Dopamine secretion• Dopamine activates pleasure
pathways of the brain, it is normally released to reward positive behaviours.
• Many drugs affect, often enhacing, dopamine activity (e.g. cocaine, heroin)
• Abuse of some drugs to lead to down-regulation of dopamine receptors, requiring higher doses to achieve same effect (habituation)
Social environment factors• The social environment plays a significant role; individuals raised
in environments with prevalent substance abuse are at a higher risk
• Peer pressure (from those that use drugs), poverty and social deprivation, traumatic life experiences and mental health all make drug addiction more likely
• Culture, religion and traditions can both positively and negatively affect addiction and this in part explains the variation in addiction patterns found between different countries
A.5.U8 Addiction can be affected by genetic predisposition, social environment and dopamine secretion.
Addiction dependence on a substance or activity resulting in its repeated and compulsive use
Learn more about addiction from the links below:
http://learn.genetics.utah.edu/content/addiction/
http://learn.genetics.utah.edu/content/addiction/mouse/
https://www.drugabuse.gov/publications/drugs-brains-behavior-science-addiction/drug-abuse-addiction
“The mentality and behaviour of drug addicts and alcoholics is wholly irrational until you understand that they are completely powerless over their addiction and unless they have structured help they have no hope.”
Russell Brand: my life without drugs
https://www.theguardian.com/culture/2013/mar/09/russell-brand-life-without-drugs
A.5.S1 Evaluation of data showing the impact of MDMA (ecstasy) on serotonin and dopamine metabolism in the brain.
The impact of MDMA (ecstasy) on serotonin and dopamine metabolism
https://www.atrainceu.com/images/img_166_designer_drugs/stimulants_in_bath_salts_NIDA.png
Mephedrone and Methylone are active components of the recreational designer drug ‘Bath Salts’
Ecstasy is a recreational designer drug that users take to feel energised, happy, to stay awake and to dance for hours. MDMA is the active component of ecstasy.The effects take about half an hour to start and tend to last between 3 to 6 hours, followed by a gradual comedown. Users often develop temporary feelings of love and affection however some users experience anxiety or panic attacks.
Saline is the control.
Serotonin is neurotransmitter that contributes to a sense of well being and happiness
The data shown in the graphs was collected from studies using rats.
A.5.S1 Evaluation of data showing the impact of MDMA (ecstasy) on serotonin and dopamine metabolism in the brain.
The impact of MDMA (ecstasy) on serotonin and dopamine metabolism
https://www.atrainceu.com/images/img_166_designer_drugs/stimulants_in_bath_salts_NIDA.png
1. Describe the effect 0.3 mg/kg (body mass) and 1.0 mg/kg of MDMA on serotonin levels over time.2. Compare and contrast the effect of MDMA on serotonin and dopamine.3. Discuss whether the changes measured are significant or not.
Nature of science: Assessing risks associated with scientific research - patient advocates will often press for the speeding up of drug approval processes, encouraging more tolerance of risk. (4.5)
https://natureofscienceib.wordpress.com/category/option-a-neurobiology/
Rigorous testing of drugs is important to determine: • Effectiveness• That it is safe to use• Appropriate dosage• Side effects
One ethical concern is that the testing process understandably takes years to complete, but during that time patients are suffering and possibly dying from a condition that a drug under development could treat. In this situation patients and their advocates will pressure health services and governments to speed-up the drug’s approval. This increases the risk of the drug causing harm to the patients.
Speeding up drug approval
http://images.techtimes.com/data/images/full/138196/ebola-treatment-research.jpg
In the US, the Food and Drug Administration (FDA) has the
responsibility to review and approve drugs for use. There are
four pathways for faster approval
Nature of science: Assessing risks associated with scientific research - patient advocates will often press for the speeding up of drug approval processes, encouraging more tolerance of risk. (4.5)
https://natureofscienceib.wordpress.com/category/option-a-neurobiology/
Rigorous testing of drugs is important to determine: • Effectiveness• That it is safe to use• Appropriate dosage• Side effects
One ethical concern is that the testing process understandably takes years to complete, but during that time patients are suffering and possibly dying from a condition that a drug under development could treat. In this situation patients and their advocates will pressure health services and governments to speed-up the drug’s approval. This increases the risk of the drug causing harm to the patients.
Speeding up drug approval
http://images.techtimes.com/data/images/full/138196/ebola-treatment-research.jpg
FDA Gives Fast Track Approval To ZMapp Experimental Ebola Drug
http://www.techtimes.com/articles/85927/20150918/fda-gives-fast-track-approval-to-zmapp-experimental-ebola-drug.htm
Experimental Ebola drug ZMapp gets fast track status from FDA
http://edition.cnn.com/2015/09/17/health/zmapp-status-fast-tracked/
The Ebola outbreak in West Africa in 2014-15 prompted the fast tracking of an experimental drug called Zmapp.
https://youtu.be/eZundDVPIYw