biol 105 sum12 lecture 5 photosynthesis and cell communication
DESCRIPTION
Lecture on Photosynthesis and Cell Communicaation.TRANSCRIPT
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Photosynthesis
Turning sunlight into cellular energy
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Photosynthesis only LOOKS like the opposite of Respiration!
6 CO2 + 12 H2O + → C6H12O6 + 6 O2 + 6 H2O
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Photosynthetic Organisms Prokaryotes: Plasma Membranes
Eukaryotes: Chloroplasts
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Why are plants Green?
Light Chlorophyll Green pigment Absorbs red, violet, blue
light Reflects green light
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Where does photosynthesis happen? Leaves Pigments
Chlorophyll a (blue green) Chlorophyll b (olive green) Carotenoids (yellow and
orange)
By absorbing slightly different λs, increase the amount of visible light that can be harvested for energy
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Chlorophyll
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Absorption and Action Spectra
1883!
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What happens to excited electrons when light hits a chlorophyll molecule?
Fluorescence Photosynthesis
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What happens in Photosynthesis?
Photosynthesis is a redox process in which H2O is oxidized and CO2 is reduced
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Steps in Photosynthesis
Light Reactions Water e- + H+ + O2
Occurs in thylakoid membranes ATP made by
chemiosmosis and photophosphorylation
Calvin Cycle CO2 sugar
Occurs in the stroma
Also called Carbon
Fixation or the “Dark Reactions”
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A. Light Reactions 1: Light Energy Splits Water
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A. Light Reactions 2: NADP+ reduced, ATP made, O2 released
Which mechanism is used to make the ATP?
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B. Calvin Cycle 1: ATP and NADPH used to fix carbon
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B. Calvin Cycle 2: carbon turned into sugar, NADP+ recycled
Sugar exported to rest of plant
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Chlorophyll a captures light energy
Photosystems 1 and 2 A photosystem (PS) has a
reaction-center complex surrounded by light-harvesting complexes
Both have chlorophyll a different environments absorb best at different λs
Reaction Center Chlorophyll
Photosystem 2: P680 Photosystem 1: P700
Photosystem structure
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Linear Electron Flow (1) Light activates an e-, which eventually
reaches P680 P680 transfers the e- to the primary e- acceptor in the reaction-center complex
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Linear Electron Flow (2) P680+ (missing an electron) is
very strong oxidizer H2O is split and P680+, reduced O2 is by-product
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Linear Electron Flow (3) The electrons “fall”
down e- transport chain (PS II PS I) H+ pumped across thylakoid membrane ATP synthesized
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Linear Electron Flow (4)
P700+ accepts e- OR excited by light
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Linear Electron Flow (5)
Another e- transport chain transfers e- to NADP+ to create NADPH
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PSII and PSI work together
NADPH carries e- to Calvin Cycle
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Making ATP by Chemiosmosis
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The Light Reactions: All Together
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Carbon Fixation: CO2 from the atmosphere is reduced (fixed) to form sugars
REQUIRES the ATP and NADPH made during light
reactions
Takes three rounds of the cycle (and 3 CO2 molecules) to make 1 sugar
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Carbon Fixation
Rubisco adds CO2 to RuBP: most abundant protein in chloroplasts; PROBABLY most abundant on Earth
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Reduction
ATP and NADPH from light reactions
Regenerates NADP+
3 CO2 make one new sugar
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Regeneration
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To make 1 G3P molecule: 9 ATP + 6 NADPH
The energy comes from the light reactions
(i.e., the sun) G3P enters many biosynthetic pathways
to be turned into other organic molecules
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Compare Metabolic Cycles
Citric Acid Cycle Catabolic pathway Oxidizes glucose to CO2
and water Synthesizes ATP
Calvin Cycle Anabolic pathway Reduces CO2 to make
complex carbohydrates Uses ATP (made in the
light reactions)
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(almost) ALL THE FOOD ON EARTH COMES FROM PHOTOSYNTHESIS Chemical Energy Carbon Skeletons (to make all organic
molecules) Chloroplasts make billions of tons of
carbohydrate each year About 50% is burned in plant mitochondria Rest is transported in plant as sucrose or
turned into cellulose and starch
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Cell Communication
Exchanging information between cells
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All Cells Communicate
Processes arose early in evolution
Same molecules found in prokaryotes and eukaryotes Recognition Mating Development
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Direct communication between cells
Cell-cell recognition
Cell junctions
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Communication via secreted signals
Local signaling Long distance signaling
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Signal Transduction
Reception Transduction Response Reset
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Reception ligand binds receptor
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Transduction Signal is amplified by a series of molecules
(second messengers)
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Response Cell reacts to signal, then it resets the signal transduction
cascade back to the starting point
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Reception requires receptors
Ligands are secreted molecules that bind receptors on or in cells
Ligand – Receptor interaction is specific: Only cells with proper receptor can receive the signal Receptor changes shape when ligand binds - activation
Receptors can be in plasma membrane OR cytoplasm
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Common Receptors
Receptors Plasma membrane
G protein-coupled receptors
Tyrosine kinase receptors
Ion channel receptors
Cytosolic
Steroid hormone receptors
Mechanisms Activate another protein
Dimerize; gain P groups
Open or close a channel
Move to nucleus and bind
DNA
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G protein-coupled receptors Structure
Function
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Tyrosine Kinase Receptors
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Ligand-gated Ion Channels
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Cytoplasmic Receptors
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Signal Transduction Cascades Amplify Signals
1 signal molecule can generate 100,000,000 response molecules in a few seconds or minutes
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Phosphorylation Cascades Adding/removing phosphate
groups can activate/inactivate proteins
Kinases: add phosphates (usually on ser or thr)
Phosphatases: remove phosphates
There is a specific kinase and phosphatase for each molecule in the cascade!
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Cytoplasmic Organization: Scaffolding proteins
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Second Messenger Systems
cAMP Ca++ and IP3
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Cellular Responses
Turn genes on or off Activate or inactivate
proteins Stimulate cell division Stimulate apoptosis
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Signals interact to fine-tune responses: Same molecule can have different effects in different cells
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Review of Cell Communication