biosensors yang yang 9/28/2004. outlines biomems enzyme-coated carbon nanotubes microcantilever...
TRANSCRIPT
BioSensors
Yang Yang
9/28/2004
Outlines
BioMEMS
Enzyme-coated carbon nanotubes
Microcantilever biosensor with environmentally responsive hydrogel
Cantilever array based nanotechnology olfactory sensors
Conclusion
BioMEMS
Inetegration of lifescience/biomedicalDisciplines with micro- and nano-Scale systems andmaterials.
BioMEMS
Materials used for fabricationBiosensors
Biosensors are analytical devicesthat combine a biologically sensitiveElement with a physical or chemical transducer to selectively and quan-Titatively detect the presence of specific compounds in a given external environment.
BioMEMSBiosensor—Mechanical detection
Mass mode cantilever sensor Stress mode cantilever sensor
BioMEMSBiosensor-- Electrical detection
Ampero, monitors formation of H+ in a redox process
Potentio, measures the potentialat an electrode in reference to another
Conducto, measures the changes in Electrical impedance between 2 electrodes. Both ampero- and potentio-
need reference electrodes,which makes the fabricationdifficult. Conducto- does not.
BioMEMSBiosensor-- Optical detection
Based on fluorescence or chemiluminescence.
Fluorescence- fluorescent markers emits light at specific wavelengths, thus any change in optical signal indicates a binding reaction.
Chemiluminescence- light is generated by the release of energy as a result of a chemical reaction.
Cited paper
R. Bashir, BioMEMS: state-of-the-art in detection, opportunities and prospects,Advanced drug delivery reviews, 56 (2004) 1565-1586
Enzyme-Coated Carbon Nanotubes
Chemical VaporDeposit(CVD)
Manufacturing method
Semi-conducting CarbonNanotubes(SWNTs)
Immobize SWNTs
Soaking and Washingin solution
Enzyme-Coated Carbon NanotubesEffects of GOx immobilization
After immobilization1. AFM height: 5 nm → 8 nm;2. 1 GOx molecule per 12 nm;3. Conductance of SWNT decreases significantly (black → cyan);
50 GOx molecules on SWNT
bare SWNT
4. pH dependent;
Enzyme-Coated Carbon Nanotubes
Effects of GOx immobilization
5. Glucose sensitive.
Pros
Very sensitive to testing entities.1. Excellent nanosize pH sensor: can measure pH as low as 0.1;2. Enzyme detector: can measure the enzymatic activity of a single redox enzyme.
Cited paperK. Besteman, et al, Enzyme-coatedCarbon nanotubes as single-Molecule biosensors, vol. 3, no. 6,727-730, 2003
Microcantilever Biosensor withEnvironmentally Responsive Hydrogel
Method of fabrication
Silicon-On Insulator (SOI) wafer
Oxide layer
Silicon layer
Buried silicon layer
Etch using photoresist mask
Etch out 3 layers at selected locations
Grown oxide
Dry etch out oxide on substrate
Use tetramethylammonium hydroxide to etch the silicon substrate and to form theCantilever/oxide composite
Cantilever/oxide combo
Soaked in hydro-Fluoric acid to etchOff all oxide
Released cantilever
Soaked in organosilanegaining bonding betweenpolymers and cantilever
Precise photo-etchingto form coated cantilever
Microcantilever Biosensor withEnvironmentally Responsive Hydrogel
Testing results
Hydrogel film capable ofSensing the change in pH;
Sensitivity of pH isPer 1 nm bending
Increasing pH path
Decreasing pH path
pH 5105
Microcantilever Biosensor withEnvironmentally Responsive Hydrogel
Pros
1. Very sensitive to changes in pH;2. Response features can be controlled by modifying swelling properties of the coating material, by changing the dimension of cantilever, etc;3. Can be testing sample specific by bonding other environmentally responsive hydrogels.
Cited paper
J.Z. Hilt, et al, Ultrasensitive biomems sensors based on microcantileversPatterned with environmentally responsive hydrogels, Biomedical microdevice5:3, 177-184, 2003
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
m1
Cantilever sensors array and polymer coatings
Cantilever length: m500
m100
thickness:
width:
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
Schematic setup
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
Data acquisition
Signal magnitude for each one of the 8 cantilevers at 5 acquisitionInstants with the injection of etha-nol
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
Data processing (Principal Compo-nents Analysis)
PCA is a statistical method that rotates a data set such that themax. variability is visible and theMost important gradients areIdentifiedmore.
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
Raw data
Differential measurement in liquids
Noises occur in liquid envir. measurement:changes in pH;Ion concentration;temperature and etc.
Compen. data
Cantilever Array Based Nanotechnology Olfactory Sensors (NOSE)
Pros and ConsReference sensors can be used for differential measurements;
It can be used in various media;
High sensitivity over a wide range of operating temperatures;
It can be integrated into other systems;
Relatively long response time.
Cited paper
M.K. Baller, et al, A cantilever array-based artificial nose, Ultramicroscopy, 82 (2000) 1-9
Conclusion
Biosensors are very small, very sensitive and
can be transplanted to human body; Dramatically improve efficiency in drug
discovery research; Multidisciplinary