blood physiology 1432 lecture 3 leucocytes 1 professor a m abdel gader md, phd, frcp (lond., edin),...
TRANSCRIPT
Blood Physiology1432
Lecture 3
Leucocytes 1
Professor A M Abdel GaderMD, PhD, FRCP (Lond., Edin), FRSH (London)
Professor of Physiology, College of Medicine &the Blood Bank, King Khalid University Hospital
King Saud UniversityRiyadh
LeucocytesWhite Blood Cells
(WBCs)
Objectives of Lecture -3At the end of this lecture the student should be able to:1.Describe the different types of WBCs2.Recognize the general functions of WBCs3.Describe the genesis and site of formation of WBCs4.Describe the stages of neutrophil formation5.Describe the role of the neutrophil in defending the body against infections6.Describe the process of phagocytosis
Leucocytes (WBCs)Contents
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis– Structure and function of Eosinophils and
basophils
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis– Structure and function of Eosinophils and
basophils
Leucocytes (WBCs)Contents
Blood Film
Hematopoiesis
Formed Elements of Blood• Red blood cells ( erythrocytes )• White blood cells ( leukocytes )
– granular leukocytes• neutrophils• eosinophils• basophils
– agranular leukocytes• lymphocytes = T cells, B cells, and natural killer
cells• monocytes
• Platelets
Leucocytes (WBCs) – cont.
General Characteristics & types of WBCs
• Types of WBC1. Granular (polymorphnuclear):
• Neutrophil 62%.– 10-16um, nucleus 2-5 lobes, purple
cytoplasmic granules
• Eosinophil 2.3%.– 12-18um, 2 lobes nucleus, coarse red
granules
• Basophil 0.4%.– 10-14um, rarely segmented nucleus,
nucleus hidden by large round bluish granules
Leucocytes (WBCs) – cont.
General Characteristics & types of WBCs
• Types of WBC2. Agranular WBC
– Monocytes 5.3%• 15-20um, kidney shape nucleus
– Lymphocyte 30%• round nucleus
– small (5-8um) – large (9-15um)
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
Leucocytes (WBCs) – cont.
Genesis (Production) of WBCs
Pluripotential stem cell
Committed Stem cell
RBCs WBCs Platelets
Mylocytic Lymphocytic Linage Linage
Figure 16-5c: Bone marrow
Hematopoiesis
21
Leucocytes (WBCs) – cont.
Genesis (Production) of WBCs- leucopoiesis)
Sites of WBC formation• Granulocytes (neutrophil, basophil,
eosinophil):
– bone marrow
• Agranulocytes – lymphocytes- bone marrow,
thymus, lymphoid tissues– monocytes- bone marrow
23
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
Life Span of WBCs
Granulocytes:• 4 to 8 hrs (transit time ) in blood circulation• 4 to 5 hrs in tissues
– In infections life span a few hours
Monocytes :– 10 to 20 hrs in blood circulation– Leave capillaries to tissues, increase in size
to become tissue macrphages which live for months
Life Span of WBCs-cont.
Lymphocytes:
• A few hrs in blood circulation >> tissues >> lymph >>> Blood (Recirculation)
?Life span: weeks to months
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils (& macrophages)
– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Leucocytes (WBCs)-cont.
Contents
Leucocytes (WBCs)
• General Characteristics & types of WBCs• Genesis (Production) of WBCs• Life Span of WBCs• Defense properties of neutrophils &
macrophages– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Defense properties of neutrophils (& macrophages)
• Attack and destroy bacteria, viruses
• Sequence of events:– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Netrophils function-cont.
Defense properties of neutrophils & macrophages-cont
Phyagocytosis: Engulfing and killing of bacteria or any
invading organism
Steps: Chemotaxis:
– Bacterial & viral toxins• Products of damaged tissues:
attract neutrophil to accumulate at infected site.
– Opsonization: plasma substances (IgG) attached to the bacteria to make them easy to phagocyte
Diapedesis
Diapedesis
http://www.whfreeman.com/immunology/CH01/diapedesis.htm
Defense properties of neutrophils & macrophages
• Attack and destroy bacteria, viruses
• Sequence of events:– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Defense properties of neutrophils & macrophages
• Attack and destroy bacteria, viruses
• Sequence of events:– Chemotaxis– Diapedesis– Amaeboid Motion– Phagocytosis
Phagocytosis- cont.
Phagocytosis is selective:Distinguish self from non-self…... How?
– Normal tissues have smooth surface– Normal tissues have protective
protein surface– Antibodies coating bacteria
(Opsonization)
Phagocytosis
Microbial killing
41
Phagocytosis by neutrophils- cont.
Neutrophils attach to bacteria & encircled it with pseudopodia and take it into a vacuole (phagosome).
• One Neutrophil can engulf 3 to 20 bacteria• One Macrophage can engulf up to 100 bacteria
Microbial killing: fusion of neutrophil granules with vacuole,
– Discharge of lysozyme, myeloperoxidase enzymes into the vacuole, killing and digesting the engulfed bacteria.
– Release of Free radicals by oxidizing agents: superoxide, hydrogen peroxide to kill the bacteria
Leucocytes (WBCs) – cont.
• Types of WBC1. Granular (polymorphnuclear):
• Neutrophil 62%.– 10-16um, nucleus 2-5 lobes, purple
cytoplasmic granules
• Eosinophil 2.3%.– 12-18um, 2 lobes nucleus, coarse red
granules
• Basophil .4%.– 10-14um, rarely segmented nucleus,
nucleus hidden by large round bluish granules
Blood Film
Eosinophils
Function: • Phagocytosis: Phagocytosis is same as neutrophil,
but less efficient
• Chemotaxis: eosinophil attracted towards chronic inflammation/allergic tissue (allergic disease of skin & lungs)
By eosinophil chemotactic factor
Phagocytose (& detoxify) antigen/antibody complexes
Eosinophils cont,
•High eosinophil count:– Parasitic (hook worm, ascaris,
bilharzia)– Allergic (asthma, rhinitis, drug
reaction)– Allergic skin diseases
Leucocytes (WBCs) – cont.
• Types of WBC1. Granular (polymorphnuclear):
• Neutrophil 62%.– 10-16um, nucleus 2-5 lobes, purple
cytoplasmic granules
• Eosinophil 2.3%.– 12-18um, 2 lobes nucleus, coarse red
granules
• Basophil .4%.– 10-14um, rarely segmented nucleus,
nucleus hidden by large round bluish granules
Leucocytes (WBCs) – cont.
• Types of WBC1. Granular (polymorphnuclear):
• Neutrophil 62%.– 10-16um, nucleus 2-5 lobes, purple
cytoplasmic granules
• Eosinophil 2.3%.– 12-18um, 2 lobes nucleus, coarse red
granules
• Basophil .4%.– 10-14um, rarely segmented nucleus,
nucleus hidden by large round bluish granules
Blood Film
Basophils
• Similar to tissue mast cells• Non-phagocytic cells• Granules: dark blue color.• Granules contain:
– Heparin– Histamine – Serotonin (5HT).
Released during allergic reactions
Objectives of Lecture -3At the end of this lecture the student should be able to:1.Describe the different types of WBCs2.Recognize the general functions of WBCs3.Describe the genesis and site of formation of WBCs4.Describe the stages of neutrophil formation5.Describe the role of the neutrophil in defending the body against infections6.Describe the process of phagocytosis
THANK YOU
جزيل لكمالشكر