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Book of abstractsSeaweed product potential: as extract and whole product








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Table of content

Preface 3

Advisory Committee: 4

Organisers, partners and sponsors 5

Normandy area & Cherbourg 7

Mont Saint Michel 8

CONFERENCE PROGRAM DAY 1 9

CONFERENCE PROGRAM DAY 2 11

List of attendees 13

Poster presentations 16

Speaker info day 1 18

Speaker info day 2 48

Press release algae Europe 65

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Preface Welcome at the 4th international Seaweed Conference in Cherbourg.

The objective of the conference is to discover the Seaweed Product Potential, with Seaweed as extract and whole product.

The conference travelled to Lower Normandy with a reason. Normandy has a great potential for economic development with a vast coastline, a network of major companies and recognized competence in research, training and innovation. The Region has invested in 2014 more than 600000 euros in research and development in this area, it also included aquaculture as priority Strategies Research and Innovation for smart specialization.

The region created the CENTRAQUA, the regional center for aquaculture. The center includes the network of teachers, researchers, industrial and institutional partners for the development of aquaculture in Lower Normandy. Production, marketing, processing and use, CENTRAQUA is involved in all levels of the industry. It offers prospective project developers a complete toolbox to support all phases of their initiative. It is a continuous exchange cell, which shares its expertise in aquaculture and its links with national and international experts. The region has local resources such as the scientific Alogobank of the University of Caen, the largest library in Europe on microalgae.

There is a considerable economic fabric area around aquaculture. Aquaculture accounts in Normandy more than 320 companies, more than 3,200 direct jobs, more than 11 000 indirect jobs, and finally a turnover of more than € 758 million. One of the strengths of the region is to have salmon in France the only two breeders that produce a premium product that stands out for its quality of aquaculture products on markets. During Seagriculture a site visit is dedicated to one of the salmon farms.

During the Seaweed conference, the following sessions are addressed to explore the potential: - Seaweed harvest and aquaculture - Market, quality and standardization - Seaweeds for humans (food, health and cosmetics) - Seaweeds for animals (feed, health, hygiene) - Seaweeds for plants (ex biostimulants and elicitor) - Green chemistry - Panel: Economics

There are scientists and corporate speakers in every session to discover the in depth background and show how products are valorized.

Enjoy the conference and your stay in Lower Normandy! the advisory committee

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The conference is organized by DLG BENELUX with an advisory committee by its side which has the following members:

Advisory Committee

Aude JOUAUX, CENTRAQUA , CRBN (France)

Isabelle Dupont Morral, CENTRAQUA , CRBN (France)

Jacques Mazoyer, Cargill (France)

Willem Brandenburg, Wageningen UR (Netherlands)

Yannick Lerat, CEVA (France)

Phillip Potin, Roscoff Biological Station (France)

Pi Nyvall Collen, Amadeite / Solmix (France)

Sébastien Pien, SMEL (France)

Stefan Kraan, Ocean Harvest Technology (Ireland)

Bela Buck, AWI - Alfred Wegener Institute (Germany)

Birgit Schmidt-Puckhaber, DLG eV (German Agriculture Society) (Germany)

Klaas Timmermans, NIOZ (Netherlands)

Paulien Hoftijzer, DLG BENELUX (Netherlands)

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Organisers, partners and sponsors DLG BENELUX

Region Basse Normandie

Cargill

Station Biologique Roscoff

Ocean Harvest Technology

Wageningen UR

CEVA

Cité de la Mer

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SMEL

NIOZ

St. Noordzeeboerderij

EABA

Olmix

Alfred Wegener Institute

Lunch partner

Contact information DLG BENELUX P.O. Box 346 3830 AJ Leusden +31 6 52011884 [email protected]

www.seagriculture.eu

VAT number: NL 8004.15.681

Chamber of Commerce: 39051673

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Normandy area & Cherbourg

Appart'City Cherbourg Centre Port 65 Avenue Carnot, 50100 Cherbourg

+33 2 36 81 32 89

Hotel Chantereyne Port de Plaisance, 50100 Cherbourg, France

+33 2 33 93 02 20

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Mont Saint Michel A great place to visit if you are around, á 2 hours’ drive.

Some history:

Mont Saint-Michel is a community with 41 inhabitants (as of January 1, 2012) in the Manche department in the Basse-Normandie, which was founded in 708. It lies on the rocky island of Mont Saint-Michel in the bay of Mont-Saint-Michel in the Wadden Sea of Normandy. The island is about a kilometer away from the coast and only about 55,000 square meters in size (circumference approx 830 meters). Nearby are the mouth of the Couesnon, the city Avranches and the border with Brittany. The landscape is Avranchin.

The non-construction 92 meters high island is known for the abbey of Mont Saint-Michel. The fortified abbey dominates the small island and is an example of French Norman architecture (beginning around 1022). In the monastery lived and worked until the 1960s Benedictine, since 2001 religious communities living there from Jerusalem.

The Mont has become a major tourist attraction, it is visited annually by about 3.5 million people. In still low, but increasing extent it is again - visited by pilgrims, among others related to the Way of St. James - as in the Middle Ages. The mountain and its bay are since 1979 a World Heritage Site by UNESCO. It is also listed since 1998 as part of the World Heritage Camino de Santiago in France.

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CONFERENCE PROGRAM DAY 1 Seaweed product potential: as extract and whole product

DAY 1 - 22nd of September 2015

Opening and key notes 9.00 Official opening of the conference by Jacques Mazoyer and Paulien Hoftijzer 9.05 Keynote: Stéphane Travert, French Deputy and Regional Councilor in charge of fisheries and marine resources 9.20 Keynote: Willem Brandenburg, Wageningen UR; The Netherlands Seaweed Cultivation – a Futuristic View 9.50 Introduction of each committee member on stage

Session 1: Seaweed harvest and aquaculture Chair: Yannick Lerat 10.00 Dr. Martial Laurans, Ifremer Brest, France Laminaria harvested in Brittany, annual trends of the fleet and improvement of the managements rules thanks to electronic equipment. 10.20 Michele Stanley, SAMS, United Kingdom Establish Marine Farming in Europe 10.40 Q&A and panel discussion

11.00 Networking Break

Session 2: Market, quality and standardization Chair: Willem Brandenburg 11.30 Julie Maguire, Indigo Rock Marine Station, Ireland An overview of the European algal industry and guide to best practice 11.50 Paz Perez, Tradecorp International, Spain Regulating seaweeds: a long journey 12.10 Yannick Lerat, CEVA, France Seaweed markets: from the Ocean of Opportunities to Real Life Economics 12.30 Q&A and panel discussion

13.00 – 14.30 Networking / Lunch break / poster sessions

Session 3: Seaweeds for humans (Food & health) Chair: Pi Nyvall Collén 14.30 Jos Hugense, Meatless, The Netherlands Hydrocolloids in food, a practical case 14.50 Maria Hayes, Teagasc Food Research Centre, the Irish Agricultural and Food Development Authority, Ireland Seaweeds as a source of functional food ingredients with enhanced health

benefits beyond human nutrition

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15.10 Willem Sodderland, Seamore, the Netherlands How to market seaweed? The business case of Seamore 15.30 Q&A and panel discussion


Session 4: Seaweeds for animals (feed, health, hygiene) Chair: Birgit Schmidt-Puckhaber 16.15 Mustapha Berri, Inra Tours, France Marine sulfated polysaccharides-riche extract exhibits antimicrobial activity and modulates cytokines expression in intestinal epithelial cells through the TLR4-Akt signalling pathway 16.30 Pi Nyvall Collén, Olmix, France Turning a problem into a resource: green algal products as a tool to reduce the use of antibiotics in animal husbandry 16.45 Håvard Steinshamn, Norwegian Institute of Bioeconomy Research (NIBIO), Norway Seaweed as a supplement to ruminants winter diet – lessons learned 17.00 Simon Faulkner, Ocean Harvest Technology, Ireland Improving fish health with OceanFeed™” 17.15 Q&A and panel discussion

17.30 - 17.45 Wrap up day one by Yannick Lerat

20.00 – 23.00 Dinner in Cité de la Mer

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DAY 2 - 23rd of September 2015

9.00: Movie Algae Sector 2050 (Green Algae Valorization)

Session 5: Seaweeds for Plants (ex biostimulants and elicitor) Chair: Anne Guiboileau and Monique Ras 9.15 Franck Hennequart, Oilean Glas Teo, Ireland Standardisation of Seaweed extracts for plant biostimulation: some insights on the industrial constraints 9.30 Dr. Anne Guiboileau, Goëmar-Arysta, France The biostimulant action and the elicitor action of seaweed extracts 9.45 Dr. Xavier Daire INRA Dijon, France The potential of algal saccharides as resistance inducers for crop protection 10.15 Dr. Gabriel Krouk, CNRS-IBIP, France How Plants respond to a combination of signals: Are Biostimulant effects triggered by a cocktail of molecules? 10.30 Q&A and panel discussion

10.45 Networking Break and poster sessions

Session 6: Green chemistry Chair: Sebastian Pien, SMEL 11.15 Hidetaka Doi, Ajinomoto, Research Institute for Bioscience Products and Fine Chemicals, Japan Method for producing l-amino acid from seaweed-derived biomass 11.30 Thierry Benvegnu, Professor of Organic Chemistry and Scientific Director of Surfact’Green®, ENSCR, France Green/Blue Surfactants and Cationized Polysaccharides from Seaweeds 11.45 Nicolas Neveux, James Cook University, Ulva ohnoi for the bioremediation of aquaculture effluents and the production of biofuels and bio-products 12.00 Q&A and panel discussion

Economics and debate 12.10 Miguel Verhein, Algasol Floating PBRs, Patented platform for low-cost, CAPEX/OPEX, and resource-efficient production of algae with industrial scalability 12.15 Debate: Economics moderated by Willem Brandenburg and Birgit Schmidt-Puckhaber

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Jeremy Brebion, ALGANACT, France Cargill, SB Roscoff, Olmix, Centr´Aqua, Batteur

13.00 Wrap up conference day 2 by Willem

13.15 Networking / Lunch break 14.00 Start/ departure site visits Salmon Farm and tour Cité de la Mer with guide (biologist)

18.00 End of the conference

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List of attendees List of attendees Seagriculture Cherbourg (Update 11 September 2015)

Company Name

Country

AFRIKELP PTY LTD K Duven South Africa Ajinomoto Hidetaka Doi Japan ALGANACT Jeremy Brebion France Algasol Verhein Miguel Bangladesh Allsea Seaweedfoods Toine Wilke the Netherlands Allsea Seaweedfoods Bojan van Weert the Netherlands Amadeite / Olmix Pi Nyvall Collen France Amber Josephine Design Productions Amber van Dillen the Netherlands Annet van Essen Annet Van Essen the Netherlands Biomark Biotechnology B.V Mark de Wit the Netherlands Cargill Jacques Mazoyer France Cargill Aurélie Loaec France Cargill Barbara Malmezat France CEVA Yannick Lerat France CEVA Pierre James France Cinvestav-Unidad Mérida México Hugol Pliego-Córtes Mexico Cinvestav-Unidad Mérida México Daniel Robledo Mexico Cité de la Mer Bernard Cauvin France Cité de la Mer Laurent Fouré France Cité de la Mer Sylvie Brieau France Cité de la Mer Virginie Brenot-Beaufrere France Cité de la Mer, Biologist Pierre-Yves Bouis France CNAM Martine Bertrand France CNRS-IBIP Dr Gabriel KROUK France CRBN Dupont Morral Isabelle France CRBN Jouaux Aude France Daithi O'Murchu Marine Research Station Julie Maguire Ireland

DIANA S.L. Langlais France DLG BENELUX Christie de Vrij the Netherlands DLG BENELUX Paulien Hoftijzer the Netherlands DLG e.V. Birgit Schmidt-Puckhaber the Netherlands ENSCR Thierry Benvegnu France French National Deputy Stéphane Travert France Gerard Roos Websites & Fotografie Gerard Roos the Netherlands Goëmar-Arysta Anne Guiboileau France GREENSEA SAS Thibaut Michel France HZ University Of Applied Sciences Jorik Creemers the Netherlands

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Ifremer Martial Laurans France IHC MTI B.V. M.B. Castro the Netherlands INRA Dijon Xavier Daire France Inrada Group Doug Kalkwarf the Netherlands Inrada Group Charles Laarhuis the Netherlands INVIVO-NSA Guyonvarch Am France Laboratory of Applied Biotechnology: Biomolecules Asma Chbani Lebanon

Laboratory of Applied Biotechnology: Biomolecules Hiba MAWLAWI Lebanon

LEI Sander Van den Burg the Netherlands LPMA Cherbourg Yann Robert France LPMA Cherbourg Students Students Students Meatless Jos Hugense the Netherlands NIBIO Havard Steinshamn Norway Nutri-Use BV Henk Schreurs the Netherlands Ocean Harvest Technology (Included in sponsor fee) Simon Faulkner Ireland

OILEAN GLAS TEO Franck Hennequart Ireland Provinciale Ontwikkelingsmaatschappij West-Vlaanderen Lien Loosvelt België

Ruitenberg Ingredients Marian Verbruggen the Netherlands Ruitenberg Ingredients Lilo de Bok the Netherlands Ruitenberg Ingredients Tomas Kuijpers the Netherlands SB Roscoff Monique Ras France Scottish Marine Institute Michele Stanley UK Seamorefood Willem Sodderland the Netherlands Seamorefood Noor Boudewijn the Netherlands Seaweed Energy Solutions AS Jon Funderud Norway Seaweed Energy Solutions AS Maren Sæther Norway Seaweed Research Unit, Faculty of Science and Natural Resources, University of Malaysia Sabah Sulaiman Olanrewaju Malyasia

SINTEF Materials and Chemistry Inga Aasen Norway SMEL Sebastian Pien France St Noordzeeboerderij Eef Brouwers the Netherlands St Noordzeeboerderij Marlies Draaisma the Netherlands TEAGASC Maria Hayes Ireland Thomas Cook University Nicolas Neveux Australia Tours INRA Mustapha Berri France Trade Corporation International, Paz Pérez Spain Université Blaise Pascal Polytech Clermont-Ferrand Cédric Delattre France

Université de Bretagne-Sud - Vannes Romain Boulho France

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Vilmorin & Cie - DS Baptiste Lelong France Wageningen UR Willem Brandenburg the Netherlands Wageningen UR Julia Wald the Netherlands Wageningen UR Willem de Visser the Netherlands Wageningen UR Paulien Harmsen the Netherlands Wageningen UR Jelle van Leeuwen the Netherlands Ad Merks the Netherlands John Benneman USA

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Poster presentations University of Caen Hatchery of alga

University of Caen Collect of a green algae ulva sp on the oysters bags

Le CNAM

Design by Amber - Amber van Dillen Sea-dish – dining without creating waste

Toine Wilkes – All Sea Seaweedfoods Seaweed enrichment of feed supplied to farm-raised Atlantic salmon (Salmo salar) is associated with higher total fatty acid and LC n-3 PUFA concentrations in fish flesh

SB Roscoff

Wageningen UR

HZ University of Applied Sciences Feasibility study cultivation of Gracilaria

Cinvestav-Merida The Mexican Seaweed Research and our cooperation program with France on extraction of bioactive compounds

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Opening and key notes 9.00 Official opening of the conference by Jacques Mazoyer and Paulien Hoftijzer 9.05 Keynote: Stéphane Travert, French Deputy and Regional Councilor in charge of fisheries and marine resources 9.20 Keynote: Willem Brandenburg, Wageningen UR; The Netherlands Seaweed Cultivation – a Futuristic View 9.50 Introduction of each committee member on stage

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Stéphane Travert French national deputy and Regional councelor of Basse-Normandie

Born on the 12th of October 1969 in Carentan, Manche, France

Education Law school – CAEN University HND business – CNED E-learning Business school – RENNES

Career 1994 - 1997: Sales executive – OCOPA - RENNES 94 – 97 1997 - 2005: Superior sales executive - ONDULYS GROUP - VPK – LOMME 2005 - 2008: Principal private Secretary of Philippe DURON – President of Basse-Normandie Region and French national deputy – CAEN 2008 - June 2012: Principal private Secretary of Philippe DURON - French national deputy and Mayor of Caen

Mandates Since 2010: - Regional councilor of Basse-Normandie in charge of Shipping, maritime resources and maritime policies

Since June 2012: - French National deputy of the third district of the Manche department - Member of the parliamentary committee of Education and Cultural affairs - Member of the Socialist group

The National Assembly

The National Assembly consists of 577 MPs, who are elected to five-year terms. Since June 2012 (the 14th Parliament), 11 MPs are elected by French citizens living outside of France. They are national officials, but also represent local constituencies, and as such, act as intermediaries between citizens, who have delegated to them part of their sovereignty, and the power of the State.

The Basse-Normandie Region (Lower Normandy)

It was created in 1956, when the Normandy region was divided into Lower Normandy and Upper Normandy. The region includes three departments, Calvados, Manche and Orne that cover the part of Normandy traditionally termed "Lower Normandy". The traditional districts of Lower Normandy include the Cotentin Peninsula and La Hague, the Campagne de Caen, the Norman Bocage, the Bessin, and the Avranchin.

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Seaweed cultivation: a futuristic view Brandenburg, W,A,

Speaker Information: Dr Ir W.A. Brandenburg Senior scientist seaweed cultivation Email: [email protected] (Mobile) phone number +31 6 20 496 396

About the author:

Willem Brandenburg is senior scientist seaweed cultivation at Agrosystem Research is. Sustainable development in combination with the big issues for the future , such as warranting food security for a fast growing human population, mitigation measures with regard to climate change, an green building blocks for the biobased industry has led him to the design of seaweed-based seafarms. The business case seaweed proteins is a central theme.

About the company/institute: Wageningen UR Agrosystems Research 6700 AA Wageningen, the Netherlands

Direct telephone number +317 480755 Agrosystems Research works on the design of sustainable agrosystems,efficient use and recycling of natural resources.

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Abstract:

In order to warrant the food security for ten billion people in about 40 years the production of proteins is essential. It happens to be that the protein content and composition of certain seaweeds is such that it is suitable for human food. With regard to the daily need of good quality protein per individual, to protein content as percentage of dry matter, we calculated that no more than 2% of the worlds’ ocean surface is sufficient to produce the proteins for 10 billion people. It is, however, still 360,000km2 of seaweed-based seafarms. In order to do it sustainable, meaning economically feasible, ecologically sound (restoring habitats) and societally accepted, we need at the same time to yield also other components like sugars, the emulsifiers, lipids, etc., we need to sequester GHGs, we need to recycle micronutrients and phosphorus, in short, all elements we already lost to the sea. We have to design seafarms in such a way that they contribute, e.g., to coastal defence.

Especially we need to think avbout the roadmap to meet these obejctives in 40 years. Therefore, it is necessary to distinguish between different scenarios:

Onshore seafarms; Nearshore seafarms Offshore seafarms

All of them with different opportunities to act in multifunctional activities at sea.

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Session 1: Seaweed harvest and aquaculture Chair: Yannick Lerat 10.00 Dr. Martial Laurans, Ifremer Brest, France Laminaria harvested in Brittany, annual trends of the fleet and improvement of the managements rules thanks to electronic equipment. 10.20 Michele Stanley, SAMS, United Kingdom Establish Marine Farming in Europe 10.40 Q&A and panel discussion


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Laminaria harvested in Brittany, annual trends of the fleet and improvement of the managements rules thanks to electronic equipment. Martial Laurans

Speaker Information Martial Laurans researcher on seaweed and crustaceans [email protected] (Mobile) phone number 00 33 2 29 00 85 02

About the author:

Laurans Marial works to Ifremer since 2005. He is involved in stock assessment for large crustaceans and Laminaria. The aims is to develop more accurate index to follow the status of resource and ensure an long term exploitation.

About the company/institute: IFREMER Technopole, Pointe du Diable 29280 PLOUZANE Direct telephone number 0033 2 98 22 40 40 www.ifremer.fr

National institute of marine research, the French public institute for marine research, Ifremer contributes, through studies and expert assessments, to knowledge about the ocean and its resources, monitoring of marine and coastal zones and the sustainable development of maritime activities.

To these ends, it designs and operates observational, experimental and monitoring tools and facilities. Ifremer manages the ocean research fleet for the French scientific community.

5 centres (Boulogne-sur-mer, Brest, Nantes, Toulon, Tahiti) and 26 stations along the entire coastline of metropolitan France and in French overseas departments and territories. 25 research departments. A set of aquaculture rearing and experimental facilities. 7 vessels (4 of them deep-sea vessels), 1 manned submersible, 1 remotely-operated vehicle for deep sea explorations, a full set of test facilities

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Abstract:

For a long time, Laminaria are harvested along the Brittany coast with a specific fleet. The main trends of this fleet are presented and analysed thanks to 40 years time series. In parallel, independent data enable to follow the state of the Laminaria digitata resource. More recently, new approaches have been developed as a statistic model to estimate the biomass of Molene Archipelago and currently a dynamic model. The aim is really to get more accurate data on the Laminaria stock and improve the management rules. In this context, the vessels are equipped with an automatic GPS. The positions of vessel are known every 1 or 5 minutes and permit to develop precise data as the daily harvest efforts and spatial distribution of harvest. In correlation with daily harvest declaration, an estimation of daily yield is calculated. All this new informations on the status of the resource are used to develop more accurate management rules which will be presented.

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Establish Marine Farming in Europe Michele Stanley

Speaker Information: Dr Michele S Stanley Senior Lecturer Email: [email protected] (Mobile) phone number

About the author:

Michele Stanley has over 21 years’ research experience in the area of biochemistry and molecular biology. She has worked on applied phycology projects for more than 17 years. Over the last 8 years, she has initiated and led research investigating marine biomass, both macro- and microalgal, as forms of biofuels at SAMS and is also developing other areas of applied research investigating the biotechnology application of algae. She a member of EPSRC’s Energy Strategic Advisory Team, a member of the Scottish Governments Science and Research Working Group for the Ministerial Group for Sustainable Aquaculture representing Blue Technology, a member of the UK Cross Research Councils Bioenergy Group, vice-chair for the Scientific Advisory Board of the Industrial Biotechnology Innovation Centre (IBioIC), a member of the steering committee for both the Aquaculture forum under Marine Alliance of Science and Technology Scotland (MASTS). She is also a member of the Society for Underwater Technology Renewable Energy Committee as well as being a Fellow for the Royal Society of Biology. About the company/institute: Scottish Assoication for Marine Science Scottish Marine Institute Oban PA37 1QA 01631 559295 www.sams.ac.uk

SAMS is (i) a learned society with a 130 year history; (ii) a National Capability delivery partner of the Natural Environment Research Council (NERC) undertaking strategic research and hosting UK national capability expertise and infrastructure for long term oceanographic time series, algal culture and scientific diving ; and (iii) an academic partner delivering undergraduate and postgraduate eduction as a part of the University of the Highlands and Islands. SAMS operates from the labs of the Scottish Marine Institute and supporting infrastructures (library, seaweed farm, research aquarium, research vessels, workshops, teaching and diving facilities). SAMS has an international reputation in marine science with

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active research in microbial & molecular biology, ecology, biogeochemistry and physics. A wide range of fundamental and applied research projects undertaken use observational, experimental, genomic, bioinformatic and chemoinformatic approaches to investigate the biology, ecology and biotechnology of microbes in coastal and ocean habitats from the tropics to the poles.

Abstract:

There is considerable interest within Europe linked to the cultivation of seaweeds initially for biofuels but now increasingly for food and biotechnology applications. What has become clear is that there is a disconnect between potential producers and the markets open to them. A key objective if this new “marine farming” industry is to establish itself within Europe is improvements in crop yield coupled with the ability to tailor the crop to market needs. Plus, although not specifically mandated at present, companies are becoming increasingly aware of the benefits of demonstrating environmental sustainability and maintenance or improvement of ecosystem services on consumer purchasing choices. Sustainable production of seaweeds for human food consumption/high value chemicals is of vital importance to the development of robust supply chains for algal products, both now and in the future. What we do know is that seaweed growth is dependent on the presence of suitable physical and chemical conditions including temperature, salinity, water motion, nutrient concentrations, carbon dioxide/ pH, light and ultra-violet radiation. This also means that the biochemical content of the seaweed will be affected by geographic origin, climate and season leading potentially to a variable final product. With enough information it may be possible to tailor seaweed biomass cultivation either through strain selection or environmental conditions for its particular market creating more opportunities for the final product. But as we see an increase in the amount of seaweed being cultivated questions now needed to be answered in terms of the potential associated risks to this form of agriculture and likely mitigation strategies.

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Session 2: Market, quality and standardization Chair: Willem Brandenburg 11.30 Julie Maguire, Indigo Rock Marine Station, Ireland An overview of the European algal industry and guide to best practice 11.50 Paz Perez, Tradecorp International, Spain Regulating seaweeds: a long journey 12.10 Yannick Lerat, CEVA, France Seaweed markets: from the Ocean of Opportunities to Real Life Economics 12.30 Q&A and panel discussion

13.00 – 14.30 Networking / Lunch break / poster sessions

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An overview of the European algal industry and guide to best practice Maguire, J., Abreu, H., Rebours, C.

Speaker Information: Dr. Julie Maguire Research Director Email: [email protected] (Mobile) phone number: +353 27 29180

About the author:

The central theme to Julie Maguire’s research has related to molluscan and macro-algal aquaculture. In 1998 she was awarded her PhD from University College Cork. She then was employed at the Seafish Industry Authority in Scotland as a shellfish research officer. Then she returned to UCC to work as a post-doctorate on an EU project investigating the impact of dredging. In 2002 she became projects officer for the Environmental Research Institute at UCC which involved project management of some of the ERI’s >200 projects. In 2005 she began work as Research Director of the Daithi O’Murchu Marine Research Station. She currently coordinates; ORION “Organic waste management by small scale anaerobic digesters” and SEABIOPLAS "Seaweeds from Sustainable Aquaculture as Feedstock for Biodegradable Bioplastics" both funded under FP7. She has also recently co-ordinated other algae projects including the Atlantic INTERREG project NETALGAE “Inter-regional network to promote sustainable development in the marine algal sector” and FP7 Marie Curie project MABFUEL “Marine algae as biomass for biofuel”.

About the company/institute: Daithi O Murchu Marine Research Station Gearhies, Bantry Cork Direct telephone number: +353 27 29181 Website: www.dommrc.com

The DOMMRS has been in operation since 1991 originally part of the Aquaculture Development Centre (ADC), University College Cork. However in 2005 it was established as an independent research station with a hatchery. It has sister companies with commercial scale shellfish and finfish farms (60ha). Research at the station has focussed on aquaculture husbandry, macro-algal culture and IMTA, disease and fouling control, biofuel production, minimising waste in the aquaculture and fisheries production process, developing new products from waste, environmental monitoring and harmful algal blooms research and

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prediction. Some current projects (not listed above) include; ACCIPHOT “Environmental Acclimation of Photosynthesis” (Marie Curie FP7-ITN Project), IDREEM “Increasing Industrial Resource Efficiency in European Mariculture” (FP7 Environment theme). This project involves the practice of Integrated Multi-trophic Aquaculture (IMTA), SAFI “Services for the Aquaculture and Fishing Industry” (FP7-Space), ODIN “Food based solutions for optimal vitamin D nutrition and health through the life cycle” (FP7 – Agriculture). Abstract:

Worldwide macro-algae production has increased by 5.7 % every year, with an annual value of $10 billion. 93% of this 15 million tons of macroalgae was produced from aquaculture. However, the European macroalgae industry is mainly based on harvesting of natural resources of macroalgae. The further expansion of the industry depends on sustainable increased access to raw material (both wild and farmed), development of valuable products and the transfer of expertise between developed and less developed regions. Therefore, the project NETALGAE created a European network of relevant stakeholders within the marine macroalgae sector. Compilation of information from different regions has resulted in a wide ranging policy study of existing practice within the macroalgae industry. Analysis of the results has established a best practice model and suggested policies for the successful, sustainable commercial utilization of marine macroalgae resources. By creating this diverse network, significant progress has been made towards developing industrial, commercial and scientific links and fostering a culture of trade and co-operation between the membership. Before there was a lack of communication vehicles, varying language capabilities, of functional contact points and limited knowledge amongst industry players of the resources and expertise that are dispersed outside of their own specific regions. A series of business tools has been developed: web-based communication portal, trade directory, training and development manuals. The project has established technology transfer initiatives and supported the formation of a European representative body for entities trading in macro-algae resources and products. Outputs Publications

General baseline study of all national algal industries across the project area (6 published reports)

Assessment of algae regulation, administration and management systems (6 published reports)

Development of best practice guidelines for management and sustainability of algae resources (1 published report)

Guidelines have included advice on: Size of plants, Seasonal rules, Spatial rules, Maximum landings, Licensing, Reporting, Management and enforcement, Traceability, Social issues, Seaweed farming, Organic Farming

7 other raising awareness documents The network

Development of a European industry database Creation of a European industry portal and business tools (www.netalgae.eu)

o 131 registrations in the network (all self registered)

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Regulating seaweeds: a long journey Pérez, P.

Speaker Information: Paz Pérez European Regulatory Manager Email: [email protected] Mobile: (+34) 673 36 63 49

About the author:

Paz Pérez is a regulatory affairs expert with broad experience in international markets. She started her career in the pharmaceutical domain, where she was involved in the registration and pharmacovigilance of medicinal products for human use, particularly in the Spanish territory. Paz moved to the agricultural sector in 2009 when she joined Tradecorp, where she provided regulatory leadership for Brazil, Mexico, Latin America, Arabic countries and the Asian-Pacific region during 4 years. She holds now the European Regulatory Manager position, leading the registration strategy of the company in the European market. She especially works to develop regulatory strategies for biostimulants, in support of sustainable agriculture. In this segment, she actively collaborates with EBIC to ensure and defend an appropriate and transparent framework for this emerging industry.

Paz holds a MSc in Industrial Pharmacy and a Master of Business Administration (MBA) degree.

About the company/institute: Tradecorp International C/ Alcalá 498, 2nd Floor 28027, Madrid (Spain) (+34) 91 327 32 00 www.tradecorp.com.es

Tradecorp is a company specialized in crop nutrition, with wide experience in micronutrients, special fertilizers and biostimulants. Founded in 1985, Tradecorp has been progressively making its presence felt on the main world markets. Purchased by the Belgian Group Sapec in 2000, our strategy is based on the value of continuity, professionalism and entrepreneurial spirit, features which Sapec has displayed throughout its more than 80-year existence. We have a complete, diversified and differentiated catalogue, with high-quality products and

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innovative solutions packages for the grower. Growers all over the world turn to Tradecorp solutions in order to maximise crop production and achieve the quality parameters that customers and the current food industry demand. We are present on all the world’s major markets and offer a customised and professional service through our several offices located in Europe, North Africa, Middle East, Asia-Pacific, Mexico, Brazil and Latin America.

Abstract:

Seaweeds are fantastic products. They are well-known for their capacity to stimulate crops, and for years have helped growers in their crop management.

There are several factors that influence the commercialization of seaweed products. But of them all, the regulatory process has become one of the most difficult to contend with.

Historically, seaweeds have been regarded as plant protection products. But the truth is that seaweeds cannot be confined to this category. Due to their properties, and most importantly, due to their mode of action, seaweeds should be considered biostimulant products. As its name states, seaweeds stimulate the plants natural processes and confer crops on an increased tolerance to abiotic stress.

In Europe's regulatory framework today, only mineral fertilisers and plant protection products benefit from a harmonized position. Thus, national laws are still the only way to market seaweeds in Europe. This situation obliges the industry to multiply their regulatory efforts, looking for compliance with the requirements of each Member State.

But its future looks promising. DG GROW (Commission's Department for Internal Market, Industry, Entrepreneurship and SMEs) is aware of this fragmented scenario. Consequently, three years ago they initiated a huge project for the revision of the fertilisers regulation to extend its scope, and eventually to embrace non-mineral fertilisers and biostimulant products that nowadays lack a common framework.

Since the beginning of the revision project, EBIC (European Biostimulants Industry Council) has worked hand in hand with DG GROW. EBIC was founded in 2011 by Tradecorp International and 10 other biostimulant producers, with a clear and shared goal: to secure an EU single market with appropriate regulation for biostimulants. Recognized as the global reference for the biostimulant industry, EBIC provides expertise on the specificities of these products, and helps the European authorities to better understand the reality of our industry. A first draft of the revised regulation is expected for this year end, and will be followed by an interservice consultation process and a full legislative procedure, after which the final regulation will be (hopefully) adopted in 2018 or 2019.

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Seaweed markets : from the Ocean of Opportunities to Real Life Economics Lerat Y1*, 1 CEVA, presqu’île de pen lann, 22610 Pleubian, France. [email protected]

Speaker Information:

Yannick LERAT CEVA [email protected]

Abstract:

Almost 500 scientific papers per month, the same for patents, this is the rate of literature production related to Seaweed and Algae. These numbers only include documents in English language, they could be multiply by two or three when including publications issued in Asian countries. By including articles in newspapers, media, blogs, social networks … numbers can be multiply by a factor 10 or 100…There is, indeed, a growing interest in Algae and Seaweed around the planet. This Ocean of information has the advantage to give a quite exhaustive view of possible applications for Algae and Seaweed biomasses, how it compares to other vegetal sources, what are technical novelties, possible advantages, opportunity of substitution of undesired petrochemical products… This motivated numerous public and private funds to be invested in R&D studies, pilots and development of new production systems and processes. Tentative roadmaps are set at various levels to help coordinating all players. All of this drives Algae and Seaweed to an Ocean of Opportunities that investors, politics and industries are currently exploring, hunting for economic value. The aim of this presentation is to navigate on this Ocean with the help of CEVA’s experts, empowered by 30 years of experience in Algae applications. To draw maps for the route from opportunities to economical values is our core business. We’ll share locations of safe harbour and mysterious island by browsing food, feed, agricultural, cosmetic applications ; ride turbulent seas by of energy, material, and chemical markets. The goal is not to deny some explored opportunity and to favor other ones, but to give pragmatic quantitative data on the time to market, technical and economic challenges for set of applications: a playground for new research projects. Will it be objective? Certainly not, but we expect that this vision shared by recognized CEVA’s experts, will bring useful maps to future navigators or at least open discussions.

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Session 3: Seaweeds for humans (Food & health) Chair: Pi Nyvall Collén 14.30 Jos Hugense, Meatless, The Netherlands Hydrocolloids in food, a practical case 14.50 Maria Hayes, Teagasc Food Research Centre, the Irish Agricultural and Food Development Authority, Ireland Seaweeds as a source of functional food ingredients with enhanced health benefits beyond human nutrition 15.10 Willem Sodderland, Seamore, the Netherlands How to market seaweed? The business case of Seamore 15.30 Q&A and panel discussion


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Hydrocolloids in food, a practical case Jos Hugense


Business address; Gebr. Spykerstraat 1, 4462 GJ Goes, Netherlands T ++31 113 271288 email; [email protected]

About the author:

Jos Hugense has more than 30 years’ experience in the meat processing industry, mainly in commercial and management positions. From 1990 to 2003 he was CEO of Hubro, a company highly specialized in cooked beef- and pork tongues. When the company was sold in 2007 it had a dominant position in its field in Holland, France and Germany. From 2003 Hugense developed a company called Meatless, an ingredient supplier to the industry. Meatless created the opportunity of a new category between meat and meat free. It was a startup, based on the idea that health and sustainability would be future drivers for the meat industry. Based on hydrocolloid technique, Meatless presents a new generation of meat extension at a much higher quality level in comparison to basic extruded products. Meatless products provide excellent taste, texture and succulence to both meat products and vegetarian products, as well as fish and dairy. The company grows at a rate of >20% per year from 2010 and is expecting to accelerate growth to even higher levels in the next five years. Meatless uses seaweed products and Hugense will give a practical view on the potential of hydrocolloids made out of seaweed in food today.

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Seaweeds as a source of functional food ingredients with enhanced health benefits beyond human nutrition Hayes, M.

Speaker Information: Dr Maria Hayes Scientific Researcher at the Teagasc Food Research Centre, the Irish Agricultural and Food Development Authority, Dublin 15, Ireland. Email:[email protected] (Mobile) phone number: +353 (0) 86 1531888

About the author:

Dr Maria Hayes joined Teagasc in 2008. She has a PhD in Microbiology and protein chemistry from the National University of Ireland, UCC and a B.Sc in Industrial Microbiology from UCD. As a researcher at Ashtown Food Research Centre, Dr Hayes’ main area of research focuses on the use of enrichment technologies for protein and valuable carbohydrate isolation as well as the development of bioassays to screen for compounds and ingredients with health beneficial activities. Dr Hayes is interested in bioactivities and bioassay development and their use in the identification of ingredients beneficial for heart and mental health and the prevention of diseases associated with metabolic syndrome including diabetes. She has worked with international collaborators from CSIC Valencia, Nottingham University (UK), University of Santiago de Compostela (Spain), Wuhan (China), France and Germany and many others. Dr Hayes also works on the Marine Functional Foods Research Initiative (MFFRI/NutraMara project), which includes collaboration with five Irish universities. She has experience in the isolation and characterisation of chitinolytic enzymes from marine waste streams and the generation and characterisation of bioactive carbohydrates, specifically chitinoligosaccharides (COS) from shellfish waste. Dr Hayes is also a task leader on a number of other research projects, including ReValueProtein and NutriCerealsIreland. These projects explore Irish meat processing streams and cereals, respectively, for recovery of high value protein based ingredients for food and non-food uses. She has authored over 40 academic, peer-reviewed international publications and is a Springer Book editor of the book: Marine Bioactive compounds: Source, Characterization and Applications.

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About the company/institute: Company/Institute name: Teagasc, The Irish Agricultural and Food Development Authority Company/Institute address: Ashtown, Dublin 15, Ireland. Postal code + city: Dublin 15, Dublin Direct telephone number: +353 1 8059957 Website : www.teagasc.ie

The Teagasc Food BioSciences laboratory leads the Irish research programme NutraMara – The Marine Functional Foods Research Initiative (www.nutramara.ie). This looks at the sustainable use of Macroalgae, Microalgae and Marine co-products for Human and Animal food use. Teagasc have developed expertise in Marine Food and Nutraceutical development especially with regards to the isolation and extraction of Proteins and by-products of this extraction process, and their chemical and bioactivity characterisation to enable their use as Functional and Health ingredients. Teagasc also employs expertise in the manipulation of these ingredients using traditional and novel processing methods to enable their innovative application and novel food product development. In addition, Teagasc has expertise in consumer acceptance and sensory analysis of novel food products. In particular, our group has expertise in novel macroalgal cereal and dairy product development and we possess a skill-set for the characterisation, up-scale production and delivery of Macroalgal proteins/peptides with proven health benefits in carrier Foods. Teagasc also coordinate the Irish Phytochemical and Food Network (www.IPFN.ie) which looks at proteins extraction from plants and their novel use as food ingredients.

Abstract:

Dr Maria Hayes, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.

It has long been established that nutrients can affect health. For example, nutrients can act on receptors in the peripheral nervous system or through direct inhibition of enzymes important in the regulation of heart and mental health and other age related diseases including diabetes and multiple sclerosis. These enzymes include but are not limited to Angiotensin Converting Enzyme-I, renin, PAF-AH, Acetyl cholinesterase (AChE), β-site APP cleaving enzyme (BACE1), Prolylendopeptidase (PEP) and Dipeptidyl peptidase 4 (DPP-IV). Bioactive peptides or cryptides are generated from food proteins by hydrolysis with proteolytic enzymes, fermentation with generally recognised as safe (GRAS) bacteria and through food processing. This presentation collates information concerning bioactive peptides and other seaweed derived ingredients and their potential health effects in relation to their ability to inhibit enzymes important in the prevention of diseases associated with heart health, circulation and mental health disorders such as AD. It details potential peptide “hits” against enzyme targets currently examined and describes known, food-derived peptides that hold potential for future development as drugs and/or functional foods. In addition, it details glycine betaine and DMSP as well as peptides and phlorotannins from seaweeds and their application as functional foods. The platforms used to extract bioactive agents from seaweeds are also discussed along with limitations concerning their application and health claims.

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Some seaweed, we seamore: the story of I sea pasta Willem Sodderland

Speaker Information: Willem Sodderland Founder & Seaweirdo [email protected] +31654777363

About the author: Willem Sodderland is an entrepreneur, marketing innovator and speaker. His latest adventure: Seamore, an ambitious food startup with a disruptive vision: to replace pasta with seaweed.After finishing Law school with an award-winning thesis on the patentability of Jurassic Park’s Dino-inventions, he entered the world of strategy consulting as a pupil of Michael Porter, at Monitor Company. He started his entrepreneurial career in 1998, launching the first incubator in The Netherlands. In 2005, he created Buzzer, Europe’s first word of mouth marketing platform in 2005. When it had become a celebrated multinational agency, Willem had built a reputation as an international marketing pioneer. While growing Seamore, he spends a small part of his energy coaching entrepreneurs, blogging about innovation and speaking at conferences through his personal platform Buzzable.

About the company/institute: Seamore Nieuwe Hemweg 2 1013 BG Amsterdam www.seamorefood.com

Seamore is a startup that has successfully launched seaweed as an alternative to pasta and created massive publicity and interest. We have the ambition to upgrade our favorite foods with a secret weapon: seaweed. And to become a key player in one of the word’s greatest opportunities: the future of food.

Abstract:

Peope active in the seaweed industry sell their favorite stuff for what it is: seaweed. But what if you don’t? What if you don’t take the weed as the starting point but people’s habits? If you embrace the lessons of innovation and how people adopt them. If you consider marketing – not meaning advertising – as the key instrument needed to successfully launch new ideas, products. Then you could create the circumstances to radically speed up the adoption of seaweed. You might even get people to exchange pasta for seaweed. Willem will show how his company has launched I sea pasta following these principles and what we can learn from other cases.

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Session 4: Seaweeds for animals (feed, health, hygiene) Chair: Birgit Schmidt-Puckhaber 16.15 Mustapha Berri, Inra Tours, France Marine sulfated polysaccharides-riche extract exhibits antimicrobial activity and modulates cytokines expression in intestinal epithelial cells through the TLR4-Akt signalling pathway 16.30 Pi Nyvall Collén, Olmix, France Turning a problem into a resource: green algal products as a tool to reduce the use of antibiotics in animal husbandry 16.45 Håvard Steinshamn, Norwegian Institute of Bioeconomy Research (NIBIO), Norway Seaweed as a supplement to ruminants winter diet – lessons learned 17.00 Simon Faulkner, Ocean Harvest Technology, Ireland Improving fish health with OceanFeed™” 17.15 Q&A and panel discussion

17.30 - 17.45 Wrap up day one by Yannick Lerat

20.00 – 23.00 Dinner in Cité de la Mer

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Marine sulfated polysaccharides-riche extract exhibits antimicrobial activity and modulates cytokines expression in intestinal epithelial cells through the TLR4-Akt signalling pathway

Speaker Information: Mustapha BERRI Position: Research engineer (pHD) Email: [email protected] (Mobile) phone number: 07 81 36 99 68 About the author: Dr. Mustapha BERRI received his PhD degree in Biochemistry in 1995 from the University of Blaise-Pascal of Clermont-Ferrand in France. He completed also a three years postdoctoral training at the University of Madison-Wisconsin (USA) in 1998, and joined the French National Institute of Agronomic Research as a biochemist to work at Lymphocytes and Mucosal Immunity laboratory. His scientific research interests involved the development and trafficking of immune cells within mucosal surfaces of pig as a model. His team have improved the scientific knowledge to elicit and enhance maternal lactogenic protection in sows to develop earlier active mucosal immunity in the neonate piglet in order to increase the animal’s resistance at the weaning, and to decrease the use of antibiotics. His research is also focusing to study the cellular and molecular host/pathogen interactions in the pig model, and to test candidate vaccines or new potential therapeutic molecules such as probiotics, prebiotics or feed additives. Because of the similarity with humans regarding the anatomy, physiology, nutrition and immunology, his laboratory is investigating to use pig as an alternative model to mice to study human’s diseases.

About the company/institute: Company/Institute name: French National Institute for Agronomic Research (INRA) UMR 1282 Infectiology and Public Health & François Rabelais University of Tours Company/Institute address: INRA Val de Loire, Route de Crotelles Postal code + city: 37 380 Nouzilly, France Direct telephone number: 33 (02) 47 42 77 00 Website: http://www.val-de-loire.inra.fr/en/Center-Researches/Animal-health-and-Public-health

The Infectious diseases and public healthjoint research unit (Infectiologie et santé Publique- ISP) is a part of the “Animal and public health” cluster of the INRA Val de Loire centre ranked among the largest European research facilities dedicated to studying animal pathogens, mainly those which are common to both humans and animals. The ISP research unit linking INRA and the University François Rabelais of Tours, aims to understand and control infectious diseases of farm animals (infectious and parasitic diseases) which represent a risk

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to public health (notably linked to consuming food of animal origin), or which are responsible for high economic losses in livestock farming sectors. The purpose of this research is therefore to understand pathogens, their mechanisms of infection and their control via the immune response. The on-going work spans the molecule to the disease. The host-pathogen relationship is thus studied at different levels, from the molecule to the cell and the whole animal.

Abstract: - Introduction

Antibiotics have been used for a long time in pig production to protect animals against pathogens. However, EU policy has been adopted to implement a sustainable production without adding antibiotics as growth promoters. Marine algae contain in their cell wall water-soluble sulfated polysaccharides with potential biological activities such as antiviral, antibacterial and immunomodulating that are explored to be used as an effective alternative to antibiotics. A marine extract of sulfated polysaccharides (MSP) was prepared from the green algae Ulva armoricana harvested in Brittany region (France) and tested for its antibacterial activity as well as its capacity to modulate the immune response.

Material and Method

Green tide algae Ulva sp. were collected on the beach at Plestin les Grèves in Britany (France), and a marine sulfated polysaccharides-rich extract (MSP) was prepared to be tested as an antibacterial compound against 40 strains of bacterial pathogens found in livestock animals. The antimicrobial activity of this extract and the MIC value were determined using mutispot inoculation assay on agar plates containing increased concentration of the extract. The stimulation of the host’s intestinal immune response mediators was evaluated using an in vitro system of porcine intestinal epithelial cells (IPEC-1). To identify the involved receptor, this extract was tested on HEK293 cell line expressing TLR4, TLR2, TLR5, TLR9, NOD1 and NOD2 receptors. We also examined the effect of MSP on the activation of different signalling patways involved in cytokine’s gene expressions (ERK1/2, p38, JNK, Akt).

Results

The incorporation of the MSP exhibited selective inhibition of tested microorganisms’ growth in a dose-response relationship compared to untreated control. Both gram-positive and gram-negative bacteria growths were affected. The most susceptible pathogens were Pasteurella, Mannheimia haemolytica, Erysipelothris, Streptococcus suis, Staphylococcus aureus and chromogenes, Bordetella brochiseptica, Corynebacterium and Actinomyces pyogenes. The treatment of polarised IPEC-1 cells with the MSP induced an up-regulation of broad range of intestinal cytokines such as TNFalpha, IL1alpha, IL1beta, IL-6, IL-8, IL12p40 and CCL20 chemokine. This stimulation of cytokine’s expression involved the Akt signalling pathway activated following the interaction of the MSP extract with TLR4 receptor.

Conclusions

Our results showed that this MSP extract could be used in animal diets to inhibit the pathogens growth and stimulate the immune response, thereby to reduce the occurrence of infections in animal herds and the subsequent use of antibiotics. This extract may also constitute a potential adjuvant in the design of mucosal vaccine approaches to improve both innate and adaptive immunity.

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Turning a problem into a resource: green algal products as a tool to reduce the use of antibiotics in animal husbandry

Speaker Information: Pi Nyvall Collén, Ph.D. Position R&D Manager Email: pnyvallcollé[email protected] (Mobile) phone number +33 6 33 61 30 46

About the author: Pi Nyvall Collén realized a master in Biology at the University of Uppsala, Sweden, followed by a thesis in Plant Physiology at Stockholm University, Sweden on starch metabolism in red algae. Seaweed have since been her organisms of choice. After a Post-doctorate period at the University of São Paulo, Brazil she arrived at the Roscoff Biological Station in 2001 where she studied different aspects of cell wall polysaccharides from brown, red and green algae as well as different algal or bacterial enzymes involved in their degradation or modification. Her experience in algae ranges from genomics, biochemistry, enzymology and physiology. She was hired as R&D manager of the Olmix group in October 2012 to coordinate the development of algal extracts and their development for plant, animal and human care.

About the company/institute: Olmix Group ZA du Haut du Bois 56580 Bréhan - FRANCE +33 (0)297 38 81 03 www.olmix.com Olmix: algae-based natural solutions. A desire to provide natural alternatives to agricultural additives led to the creation of Olmix Group in Bréhan, at the heart of Brittany, in 1995. In 20 years, the company has become one of the major global specialists in marine biotechnology and green chemistry. Olmix Group brings natural sources of nutrition and good health to animals, plants and people, for a complete, consistent food and health chain. Olmix Group employs 320 people and has a turnover of EUR 66 million in 2014, 80% of them of sales exported. Olmix commercial structure is based on a network of 21 establishments covering more than 100 countries on the five continents. The company, which is listed on the Paris Stock Exchange, has seven production sites in Europe and its innovative character, in tune with the change of environmental regulations in the world, constitutes an important reference in sustainable development.

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Abstract: The green seaweeds Ulva armoricana and Ulva rotundata (Ulvophyceae) are widespread on the coasts of Brittany, France and are a regular cause of green tides along the Breton intertidal. The green tides represent 200 000 tons of algae of which a part are caught on beaches and mudflats. The surface affected by the strandings vary between 2 000 and 4 000 ha. The Ulvans program, financed by BPI France, was launched in 2012 with the aim to develop an entire valorization chain based on the green tide biomass, developing the tools and processes for harvesting, pretreatment and extraction to allow formulation and commercialization of algae based products for agriculture. Based on the extracts developed by the Ulvans program a novel range of products for animal health have been formulated to be distributed via the drinking water. The range include products for immune stimulation, improvement of digestive health and for digestive stimulation. The influence of these Algae-based seaweed products have been extensively tested in trials on poultry and pigs in commercial farm conditions, both in France and abroad, with positive effects on zootechnical and economical performances. A focus will be made on the results using the immune stimulating product, SEARUP, in poultry where trials have been réalised with pullets as well as layers and broilers demonstrating its impact on the immune system. The protocol used for the trials are based on the comparison of one control house (using standard farm prophylaxis) and one test house (using “algae” prophylaxis) at the same farm. 60 000 pullets were included in the trial and the impact on mortality, feed consumption and antibody titers was examined. Mortality due to crowding was significantly reduced (145 and 102 in the two control groups and 9 in the treated group). A tendency of increase in the antibody titers is observed in combination with a reduction in the coefficients of variation (CV) of the titer for the SearUp treated animals. The trial on laying hens was realised in Brittany on 55 000 animals/group. The impact on egg number and weight, layer weight and antibody titers were examined. A significant improvement of the number of eggs/ layer were observed (75,35 eggs/ layer in the treated group and 74,56 in the control group). Antibody titers showed a slight increase in combination with a reduction of the coefficients of variation (CV) for the SearUp treated animals. The broiler trial was conducted on over 450 000 day old chickens distributed in sixteen houses in each of eight farms participating in the trial. A significant reduction was observed of the CV of the antibodytiters of Searup treated animals. In conclusion, irregardless of type of poultry production SEARUP improve immune system performance reducing the CV of the antibody titer providing a more homogenous vaccination and improved protection.

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Seaweed as a supplement to ruminants winter diet – lessons learned Steinshamn, H. & Novoa-Garrido, M.

Speaker Information: Håvard Steinshamn Senior Researcher [email protected] (Mobile) phone number : +47 404 80 314

About the author:

Håvard Steinshamn is a grassland scientist with interest in forage production and ruminant nutrition, particularly in organic managed systems. Recent research focus has been on grassland management, the relationship between grassland management and ruminant productivity and product quality, and replacement of synthetic vitamins with natural sources. Steinshamn co-ordniated a national funded project on the use of macro-algae as a supplement to ruminant and is currently involved in a new national funded research project (Promac) on the utilization of seaweed as food and feed. His task in Promac is to assess extracts from macro-algae as protein feed supplement to ruminants.

About the company/institute: Norwegian Institute of Bioeconomy Research (NIBIO), Department of Grassland and Forage Sdience Fredrik A Dahls vei 20 1430 Ås +47404 80 314

Bioforsk, the Norwegian Institute for Agricultural and Environmental Research, conducts applied and specifically targeted research linked to multifunctional agriculture and rural development, plant sciences, environmental protection and natural resource management. Bioforsk's objective is to provide industries, governments and consumers with new knowledge, services and solutions within these scientific fields. International collaboration is given high priority. From July 1.st 2015 the three research institutes Bioforsk, Skog og landskap and Norsk institutt for landbruksøkonomisk forskning (NILF) will be merged into a new institute: Norwegian Institute of Bioeconomy Research – NIBIO.

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Abstract:

Seaweeds have been used as supplementary feed for livestock in Norway for centuries. Research activities on the use of seaweed as feed started early last century and continued until the late 1960s. The results were elusive, partly because the design of the experiments were imperfect. However, a long term experiment in the 1960’s demonstrated 6% higher milk production by cows supplemented mineral fortified Ascophyllum nodosum meal than in cows offered standard mineral supplement. The authors suggested that seaweed compounds might have had benficial effect on the rumen microflora. Seaweeds are a rich source of Se and antioxidants such as substituted phenols, polyphenols, vitamins, and vitamin precursors. Results from research last 10-20 years suggests that dietary supplementation with A. nodosum meal has positive effects on ruminant product quality and stress tolerance. Alginates have been documented to be non-specific immunostimulants.

A. nodosum is currently commersially harvested and processed and sold as a feed supplement. Winter fed sheep and cattle in Norway needs to be given extra fat soluble vitamins and minerals, particularly vitamin E and Se, in order to ensure good animal health and production. Based on the aquired knowledge from international reseach on A. nodosum and its possible beneficial health effect, we tested if A. nodosum has immunestimulating effect and can be used as a substitute for synthetic vitamin E in sheep and cattle. Our hypothesis were that supplementing the diet with seaweed to sheep and lactating dairy cows would produce better adaptive immune response following immunization compared to no supplementation and similar to animals given extra vitamin E. Two feeding experiments were conducted, one continous with 40 pregnant ewes and one with 24 lactating dairy cows in a replicated Latin square design. The four supplement treatments applied were: A. Nodosum meal (SW), Natural vitamin E, Synthetic vitamin E, or Control. The average daily rate of A. Nodosum meal per ewe and cow in SW was 80 and 200 g DM, respectively. The ewes and their newborn lambs were monitored the entire indoor feeding period, from mating until pasture let out (200 d). In the ewes, supplementation with SW had no health effects compared to the other treatments, and serum IgG concentrations were reduced in the SW group.The adaptive immunity of the lambs was not affected by supplementation, and seaweed reduced the counts of different intestinal bacterial groups. However, seaweed interferred with the lambs passive immunity resulting in a mortality rate of 35 %, compared with 10% in Control. All cows responded well to immunization, but there were no significant effects of the diet on the immune response measured. The immunesupression observed in newborn lambs from ewes offered SW was likely du to impaired Ig absorption from colostrum, and we conclude that ruminants should not be supplemented with seaweed during peripartum. More research is needed on the identification of bioactive components in seaweed, their effects in animal health, the mechanisms related to their effects on the animal health. and testing before seaweed should be used as a feed supplement to ruminants.

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Improving fish health with OceanFeed™ Simon Faulkner & Stefan Kraan

Speaker Information: Dr Simon Faulkner Senior Scientist Email: [email protected] (Mobile) phone number: 09351807

About the author:

Simon graduated with a first class honours degree in applied biopharmaceutical and healthcare science from Galway-Mayo Institute of Technology in 2008 and was awarded a Walsh fellowship to complete a PhD in collaboration with Teagasc and University College Dublin. Simon graduated with a PhD in reproductive biology in December 2012 and joined Ocean Harvest Technology in Milltown, Co. Galway. Since then, Simon has worked as a scientist, bridging the gap between academia and industry. In December 2013, Simon graduated with a postgraduate diploma in management practice from the UCD Michael Smurfit Graduate Business School. Simon is currently responsible for a number of projects which involve the development of new products for the animal feed, human food and cosmetic sectors. These products are all based on specific bioactive ingredients that are present in various seaweed species. Simon’s main areas of expertise include seaweed and nutrition, the extraction of various components from seaweed, seaweed and animal health and new product development.

About the company/institute: Ocean Harvest Technology Ltd Milltown Business Park, Milltown, Tuam, Co. Galway, Ireland H54 D722. (Co. Galway) Direct telephone number 09351807 Website www.oceanharvest.ie

Ocean Harvest Technology was founded in 2005 by Dr. Stefan Kraan and Patrick Martin and has grown from a single headquarters in Galway, Ireland to having a strong presence in North America, Asia, Australia and Europe. Ocean Harvest Technology supplies patented seaweed-derived formulas to aquaculture, human food and pet industries as well as providing macroalgae extracts for various applications. The formulas are based on the bioactive ingredients found in different seaweed species and, in addition to providing various health benefits, these formulas can help to replace the synthetic ingredients found in feeds in a sustainable manner.

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Abstract:

Ocean Harvest Technology produces a number of seaweed blends for various applications. These blends include OceanFeed ™ Salmon, Swine, Equine, Bovine, Shrimp and OceanFeed™ Pet. The blends utilise selected seaweed species, sourced worldwide, with a favourable composition and bioactive properties. In general, seaweeds are a good source of carbohydrates protein and minerals. In addition to being an excellent source of minerals, trace elements and some vitamins, the OceanFeed™ range can offer additional functional benefits for the animal. For instance, OceanFeed™ has been shown to favourably influence the fatty acid profile of Salmon flesh and reduce susceptibility to infection. This product has allowed farmers to at least partially replace some feed components with OceanFeed™ thus reducing reliance on some undesirable ingredients. In addition, OceanFeed™ represents a sustainable feed source which is significant, given that the global population is on the rise and with it the challenge to continually produce high-quality and safe products.

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9.00: Movie Algae Sector 2050 (Green Algae Valorization)

Session 5: Seaweeds for Plants (ex biostimulants and elicitor) Chair: Anne Guiboileau and Monique Ras 9.15 Franck Hennequart, Oilean Glas Teo, Ireland Standardisation of Seaweed extracts for plant biostimulation: some insights on the industrial constraints 9.30 Dr. Anne Guiboileau, Goëmar-Arysta, France The biostimulant action and the elicitor action of seaweed extracts 9.45 Dr. Xavier Daire INRA Dijon, France The potential of algal saccharides as resistance inducers for crop protection 10.15 Dr. Gabriel Krouk, CNRS-IBIP, France How Plants respond to a combination of signals: Are Biostimulant effects triggered by a cocktail of molecules? 10.30 Q&A and panel discussion


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Standardisation of Seaweed extracts for plant biostimulation: some insights on the industrial constraints

Speaker Information: Name and titles if applicable: Mr. Franck Hennequart Position Seaweed Product Global Manager Email: [email protected] (Mobile) phone number +33 6 33 34 66 88

About the author:

Franck hennequart is an Engineer, graduated in the Marine Biology and Biotechnology Laboratory of the University of Caen (Normandy, France), specialised in the valorisation of marine resources. Franck initiated his career in France with several aquaculture research and lecturing contracts; He worked then for 3 years in British Columbia in Canada as R&D project assistant for a Provincial organisation promoting partnership between Industry and Research groups for marine technological transfer. Back in Europe, Franck worked as a consultant for the seaweed industry and has been then appointed Project Coordinator in the Molecular Glycobiotechnology Group of the Dept. of Biochemistry at National University of Ireland Galway. In parallel, he participated to the creation and the setup of OGT, an Irish company developing novel seaweed extracts. After 5 years in his academic position, Franck has then joined OGT full time, where he was the Technical Director. His role in OGT was to assist technical and new product development as well as support any client towards technical applications. Now, Franck works in the Marketing and R&D Department in Tradecorp as global Seaweed Product Manager. He is also share-holder of a French consulting company called SEANEO specialised in marine environment impact studies and Marine Protected Areas.

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About the company/institute: Oilean Glas Teoranta (OGT) – a Tradecorp company Ballymoon Industrial Estate, Kilcar, Co. Donegal, Ireland +33 6 33 34 66 88 http://ogt.ie/

OGT is an Irish company created about 10 years ago and designed around a unique gentle extraction industrial technique to produce high quality fresh seaweed extract from fresh hand harvested Ascophyllum nodosum. Developping speciality seaweed based products for sport turf and high value agricultural crops, OGT has grown up to 24 collaborators selling product in more than 20 countries over the world in June 2014. About a year ago, OGT has been acquired by the Spanish company Tradecorp International, specialised in plant nutrition and part of the SAPEC Group.

Abstract:

Biostimulation of leaving organisms is based on the initiation of specific physiological processes within these organisms by active compounds. In most of the cases, the main goal of biostimulation is to improve the general physiology towards a situation related to a stressed environment. Plants can thus also be biostimulated using different types of natural and non-natural compounds. Recent evolutions in science and analytical techniques have greatly evolved understand some of the physiological mechanisms involved. Seaweed extracts, among other biostimulants have passed from the “snake oil” status to fertilisers for many years. Indeed, few compounds naturally present in seaweeds are well identified as key active agents on those plant effects.

However, seaweeds being natural organisms are subjected to natural variations, even intra-specific, which forces seaweed processors to adapt to it. Indeed, both at the harvesting level and the processing levels, the producers need to implement some controls and protocols in order to limit the effect of those natural variations on their end product. This is the only way to further development of this industry and globalisation of the use of seaweed extracts as plant biostimulant, both on technical terms and regulatory terms.

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The biostimulant action and the elicitor action of seaweed extracts Guiboileau, A.

Speaker Information Anne GUIBOILEAU, Ph.D. BioSolutions Biosolution R&D Laboratory Manager Laboratoires GOËMAR – ARYSTA Email: [email protected] (Mobile) phone number: +33 6 76 10 88 11

About the author:

CURRENT POSITION – FORMER EXPERIENCES 2011 now 2008 2011 2007 2008

Biosolution R&D Laboratory Manager – Laboratoires GOËMAR (France) - ARYSTA Assay of efficacy of new molecules in plant physiology, plant nutrition and biocontrol Biostimulant and biocontrol knowledge

PhD at INRA Versailles (France) Autophagy and nitrogen recycling in Arabidopsis thaliana

Research project at INRA Caen (France)

Nitrate uptake regulation in oilseed rape

DIPLOMA 2008 2011 2007 2008 2006 2007

PhD at Université Paris-Sud XI and INRA Versailles Second year of Master degree on plant physiology done in University of Caen First year of Master degree on plant physiology done in Finland

About the company/institute: Goëmar Parc Technopolitain Atalante, CS41908 35435 SAINT-MALO Cedex +33 2 99 19 19 19 www.goemar.com and arystalifescience.com

Goëmar, based in Saint-Malo, France, develops Bioperformance technologies for crop production, to support a positive agricultural model. These Biostimulant, Innovative Nutrition and Biocontrol technologies get the most out of plant's natural forces and their environnement to stimulate, feed, protect crops and meet growers’ needs. With sales in over 40 countries, Goëmar is an innovative company, investing every year more than 10 % of its turnover in research and development.

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Abstract:

The challenge of modern agriculture is to adapt itself to the worldwide population increase by upgrading yield while preserving the environment. Methods for achieving this goal are to improve the intrinsic capacity of plants. Even if plants are static organisms, they have developed high performed mechanisms to protect themselves against biotic or abiotic attacks. The idea is to stimulate these mechanisms in order to have more robust plants and more yield.

Plants cells are able to recognize some molecules called plant resistance inducers or elicitors. These molecules can be endogenous or external. Laminarin is an oligosaccharide extracted from the seaweed Laminaria digitata. This external elicitor triggers plant natural resistance via the induction of plant resistance mechanisms. In fact, it has been shown that laminarin is rapidly recognized by plant cells; this recognition is characterised by membrane ionic flux stimulation. It has also been demonstrated that phenylpropanoid and oxylipin pathways are stimulated by laminarin application. Laminarin contributes to different plant diseases resistance, as apple scab or botrytis on strawberry.

Another method used to improve plant growth, yield and quality parameters is the use of biostimulants such as seaweed filtrates. In this domain, some of their modes of action have been elucidated. Nitrogen is a very important element for plants to produce proteins, nucleic acids and chlorophyll. Nitrate is absorbed from the soil and represents the major source of nitrogen for the plant. The nitrate uptake is performed by nitrate transporters belonging to the NRT family. Then, nitrate is converted to nitrite by the nitrate reductase. It has been shown that plants treated with seaweed extracts present a higher activity of this enzyme. Phosphorus is also an important macro-nutrient. Tomato plants treated with seaweed extracts present a higher phosphatases activity. Iron is participating to the chlorophyll synthesis and represents an important micro-element. Seaweed extract applications on plants increase the ferric chelate reductase activity. However, the bioactive ingredients have not been well identified and their mechanisms of action remain not enough understood. The aim of our ongoing project is first to determine, using a metabolomics profiling of seaweed filtrates, the bioactive compounds responsible for the plant growth. Then, the physiological effects of these seaweed extracts will be studied in the plant model Arabidopsis thaliana through transcriptomic and metabolomics approaches.

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The potential of algal saccharides as resistance inducers for crop protection Daire, X.

Speaker Information: Daire Xavier. PhD. Researcher at INRA UMR 1347 Agroécologie AgroSup-INRA-uB [email protected]

About the author:

X. Daire is a phytopathologist. He has been studying for 15 years the mode of action of saccharidic as resistance inducers against plant pathogens.One of his main objective is to bridge the gap between basic and applied research. About the company/institute: Institut national de la recherche agronomique: 17, rue Sully 21 065 Dijon cedex 0033 3 80 69 31 04 www.inra.fr INRA is the french public institute of research dedicated to food and agriculture

Abstract:

Algal polysaccharides have been known as elicitors of terrestrian plant defenses since the early 2000s. Studies focused on laminarin, ulvans and alginates. These compounds trigger a typical defense syndrome in various plant species. That is to say, they elicit defense reactions such ions fluxes, ROS production, MAPK activation, upregulation of defense genes and production of antimicrobial compounds.

Foliar application of these saccharides can induce resistance against plant pathogens. Thus, they are commonly considered as microbial associated molecular patterns (MAMP) although their cognate receptors (PRR) in plant remain unknown so far.

Commercial resistance inducers with algal polysaccharides as active ingredients have been developed (e. g. laminarin). They represent an attractive means for crop protection, without any detrimental effect on health and environment. Polysaccharide-based products can provide interesting level of disease control in certain cases. Factors affecting their overall performance will be discussed during the presentation.

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How Plants respond to a combination of signals: Are Biostimulant effects triggered by a cocktail of molecules? Krouk, G.

Speaker Information: Dr Krouk Gabriel CNRS researcher Email: [email protected] (Mobile) phone number +33499612937

About the author:

I'm a French CNRS Researcher working in B&PMP lab (Montpellier France) applying Systems Biology* in order to decipher how nutrient related signaling pathways influence plant growth and development. https://sites.google.com/site/gabrielkroukresearch/

About the company/institute: Company/Institute name Institut Claude Grignon,IBIP, B&PMP Company/Institute address 2 place pierre viala Postal code + city 34060 Montpellier Cedex Direct telephone number +33499612937 Website https://sites.google.com/site/gabrielkroukresearch/

Abstract:

As sessile organisms plants have to adapt to varying external conditions. Plants perceive their environment and try to modify their metabolism and growth accordingly. But what is actually perceived as a signal by plants? Is it a single molecule? Or is a combination of molecules?

Even if molecular receptors of single molecules have been identified so far (ex: hormones [auxin, cytokinins, etc.], nitrate, phosphate…), I will show that plants are able to detect and interpret combinations of signaling molecules.

In particular we have been investigating hormone vs. nitrogen interactions; as well as nitrate and phosphate signaling interactions. This recent field of investigations will be presented and discussed in the context of Biostimulant potential effects. In other words, are Biostimulant effects triggered by a cocktail of molecules?

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Session 6: Green chemistry Chair: Sebastian Pien, SMEL 11.15 Hidetaka Doi, Ajinomoto, Research Institute for Bioscience Products and Fine Chemicals, Japan Method for producing l-amino acid from seaweed-derived biomass 11.30 Thierry Benvegnu, Professor of Organic Chemistry and Scientific Director of Surfact’Green®, ENSCR, France Green/Blue Surfactants and Cationized Polysaccharides from Seaweeds 11.45 Nicolas Neveux, James Cook University, Ulva ohnoi for the bioremediation of aquaculture effluents and the production of biofuels and bio-products 12.00 Q&A and panel discussion

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The potential of algal saccharides as resistance inducers for crop protection Doi, H., Chinen, A., Fukuda, H., Usuda, Y.

Speaker Information: Hidetaka Doi, Master of Science Chief Researcher Email: [email protected]

About the author:

Hidetaka Doi started his career in Ajinomoto Co.,Inc as a microbial metabolic engineer after getting a master degree in The University of Tokyo, Japan in 2005.

About the company/institute: Company/Institute name Ajinomoto Co., Inc. Company/Institute address1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan Postal code + city 210-8681 Direct telephone number +81-44-210-5714 Website http://www.ajinomoto.com/en/

Ajinomoto Group is one of the biggest Japanese Food and Seasoning companies (Foundation : 20 May 1909

Number of Employees : 3,398(Unit), 27,579(Consolidated)). The objective of Ajinomoto Group is to be "A group of companies that contributes to human health globally" by continually creating unique value to benefit customers.

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Abstract:

Amino acids are mainly made from landcrops and terrestrial sugars over million metric tons scale per year using fermentation technology. Ordinary amino acid fermentation is one of biggest consumers of sugars and corns. Recent studies suggested that red algal agarose and brown algal alginate are candidates for novel sustainable resources for fermentative production (Wargacki et al., 2012 and Youngdeuk et al., 2013). Thus we started to isolate a novel Vibrio strain which could degradate and assimilate both agarose and alginate from the gut of a Japanese turban shell (Turbo cornutus). We succeeded in isolating a novel Vibrio sp. strain which could degradate and assimilate both agarose and alginate (SA2T =DSM29824T =NBRC111146T). Using genetically modified Vibrio sp. SA2 strain, we could accumulate L-Glutamate from seaweed biomass (Patent Number:WO/2015/041264). Whole genome analyzing of Vibrio sp. SA2 strain and metabolic engineering technique enabled us to degradate and bioconvert seaweed biomass to amino acids with other host microbial species (Patent Number:WO/2015/041265). In this presentation, we would like to introduce our research trial of seaweed biomass fermentation for producing amino acids.

References

Wargacki, A.J. et al., (2012). An engineered microbial platform for direct biofuel production from brown macroalgae. Science. 335, 308-313.

Youngdeuk, L. et al., (2013). Molecular cloning, overexpression, and enzymatic characterization of glycosyl hydrolase family 16 β-Agarase from marine bacterium Saccharophagus sp. AG21 in Escherichia coli. Journal of Microbiology and Biotechnology. 23, 913-22.

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Ulva ohnoi for the bioremediation of aquaculture effluents and the production of biofuels and bio-products

N. Neveux*1, M. Magnusson1, A. Cole1, A. Angell1, C. Carl1, R. Lawton1, L. Mata1, D. Roberts1, A.K. Yuen2, B.S. Haynes2, N.A. Paul1, T. Maschmeyer2, R. de Nys1 1 James Cook University, Australia; 2 The University of Sydney, Australia


Nicolas Neveux.

Bachelor in Biological Sciences at the University of Nice, France.

Master Degree in Aquaculture at the University of Montpellier, France.

Research Assistant with MACRO – the Centre for Macroalgal Resources & Biotechnology at James Cook University, Australia.

Doctoral Degree in Aquaculture (culture of macroalgae for the production of liquid biofuels) with MACRO – the Centre for Macroalgal Resources & Biotechnology at James Cook University, Australia.

Currently employed as a Research Scientist with MACRO – the Centre for Macroalgal Resources & Biotechnology at James Cook University, Australia.

[email protected]

About the company/institute:

MACRO group.

Our R&D includes the assessment of valuable bioproducts and biotechnologies, based on the unique biochemistry and properties of macroalgae, and the co-location of algal production with existing industries for bioremediation (treating waste streams). We utilise the broad distribution of both marine and freshwater macroalgae and the ability of species to grow in industrial waste water. Our goal is to develop and commercialise marine and freshwater macroalgae for fuel, feed and fertiliser applications (https://research.jcu.edu.au/macro). We do this through a strong industry partnership with MBD Energy and support from the Australian Federal Government. Our research group was recently awarded the Excellence in Sustainable Water Management Prize by the United Nations Association of Australia at the World Environment Day Awards 2015 for our work on the treatment of urban, agricultural and industrial wastewaters using macroalgae.

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Abstract:

The integrated production of seaweeds in land-based aquaculture systems in tropical Australia is unique due to the interaction between an expanding aquaculture industry and stringent regulations for the release of effluent water to the environment of the Great Barrier Reef.

Aquaculture effluent released by intensive land-based farms in Northern Australia must now achieve zero-nett discharge of nutrients to the receiving environment. Notably, this effluent is a resource for the cultivation of seaweeds in intensive land-based systems which reduce nutrients to meet compliance before discharge. This method relies on the selection of reliable species of seaweed that grow and thrive in this tropical environment to deliver a high productivity of biomass for bio-products.

We have developed, with industry partner MBD Energy, a functional and scalable high-rate algal pond (HRAP) system for the production of Ulva ohnoi. This system is integrated within the production framework of a 100 ha (1000 tonnes p.a.) prawn farm (Pacific Reef Fisheries) and provides compliance with effluent discharge regulations and sustainable year-round production of U. ohnoi. In parallel we have developed the market potential of U. ohnoi as a nutraceutical (soluble fibres), a fertiliser (biochar, soil conditioner), and as a feedstock for renewable fuels (biocrude) to improve the economics of the overall process.

The aim in this conference is to present the latest results for the cultivation of U.ohnoi in HRAPs using aquaculture effluents as a source of nutrients, and the conversion of this biomass to liquid biocrude and other commodities.

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Green/Blue Surfactants and Cationized Polysaccharides from Seaweeds Benvegnu, T., Pessel, F., Covis, R., Sassi, J. F., Lerat, Y., Benoit, M.

Speaker Information; BENVEGNU Thierry Professor of Organic Chemistry and Scientific Director of Surfact’Green® [email protected] Tél. 33 2 23 23 80 60 About the author: Thierry Benvegnu was born in Pau (France) in 1966. He graduated in chemistry and chemical engineering from the “Ecole Nationale Supérieure de Chimie de Rennes” (France, 1989) and obtained his Ph.D. at the University of Rennes (1993). After postdoctoral studies with Prof. P. A. Wender at the University of Stanford (USA, 1993–1994), he obtained an academic position as a lecturer at the ENSCR. He was appointed Professor in Organic Chemistry in Rennes (ENSCR, 2004). His research topics deal with the synthesis and the physicochemical evaluation of archaeal membrane lipids as innovative drug/gene delivery systems and in “green” synthesis of biocompatible and biodegradable surfactants from renewable raw materials. He is Scientific Director of the Business unit Surfact’Green® specialized in the production and formulation of Green/Blue Surfactants.

About the company/institute: - Ecole Nationale Supérieure de Chimie de Rennes UMR CNRS 6226 Equipe Chimie Organique & Supramoléculaire 11, Allée de Beaulieu – CS 50837 35708 Rennes Cedex 7 France Tél. 33 2 23 23 80 00 www.ensc-rennes.fr Founded in 1919, the École nationale supérieure de chimie de Rennes (ENSCR) (Rennes School of Chemistry) trains chemical engineers for industry and offers study programs from undergraduate to PhD level.

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- Business unit Surfact’Green® Ecole Nationale Supérieure de Chimie de Rennes 11, Allée de Beaulieu – CS 50837 35708 Rennes Cedex 7 France Tél. 33 2 23 23 80 60 www.surfact-green.com Surfact’Green® is a business unit specialized in the eco-friendly production and in formulation of original eco-compatible surfactants derived from Biomass

Abstract: The development of green surfactants based on natural renewable resources is a concept that is gaining recognition in detergents and cosmetics. This new class of biodegradable and biocompatible products is a response to the increasing consumer demand for products that are both "greener", milder and more efficient. In order to achieve these objectives, it is necessary to use renewable low-cost biomass that is available in large quantities and to design through green processes molecular structures that show improved performance, favourable ecotoxicological properties and reduced environmental impact. Within this context, marine algae represent a rich source of complex polysaccharides and oligosaccharides with innovative structures and functional properties that may find applications as starting materials for the development of green/blue surfactants and cosmetic actives. The Ecole Nationale Supérieure de Chimie de Rennes (ENSCR) and the Centre d’Etude et de Valorisation des Algues (CEVA) in Brittany (France) have developed original surfactants based on alginates (cell-wall polyuronic acids from brown seaweeds) or ulvans (sulfated rhamnouronans from the cell wall of green seaweeds) and fatty hydrocarbon chains derived from vegetable resources.1 Controlled chemical and/or enzymatic depolymerizations of the algal polysaccharides give saturated and/or unsaturated functional oligosaccharides incorporating rare sugars such as uronic acids (mannuronic, guluronic, iduronic, glucuronic acids) and sulphated rhamnose. The functionalization of these oligosaccharides through transesterification / transglycosylation processes in fatty alcohols is solvent-free and yields anomerically pure derivatives. Aqueous basic and acid treatments lead to anionic or neutral single-tailed surfactants. The industrial development of these technologies is in progress within the business unit Surfact’Green®. Cationized polysaccharides through covalent linkages or ionic interactions with surfactants were also developed to exhibit interesting rheological properties and to provide original polysaccharide/surfactant hybrid complexes.2

1. Benvegnu, T., Sassi, J.-F., Topics in Current Chemistry, 294 (2010), 143–164. 2. Covis, R., Vives, T., Gaillard, C., Benoit, M., Benvegnu, T., Carbohydrate Polymers, 121

(2015) 436–448.

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Economics and debate

12.10 Miguel Verhein, Algasol Floating PBRs, Patented platform for low-cost, CAPEX/OPEX, and resource-

efficient production of algae with industrial scalability

12.15 Debate: Economics moderated by Willem Brandenburg and Birgit Schmidt-Puckhaber

Jeremy Brebion, ALGANACT, France Cargill, Joris Peters

SB Roscoff Olmix

Centr´Aqua, Batteur

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Floating PBRs Patented platform for low-cost, CAPEX/OPEX, and resource-efficient production of algae with industrial scalability

Speaker Information: Miguel Verhein Position Executive Director Email: [email protected] (Mobile) phone number: +34609601000

About the author:

Miguel Verhein, Executive Director of Algasol. Part of the Management team and responsible for the commercialization of a proprietary low-cost photobioreactor technology for cultivation of micro algae. Primary responsibilities include: • Technology (Inventor) • Communication, Government Relations • Purchasing Miguel has an economic background from the Stockholm University in Sweden. Miguel has led and completed a number of successful startups in many different areas, ranging from crude oil to complex technical inventions within the food industry. Miguel's diversified background also encompasses several patents. The life-long love and work for the environment has peaked during the last 24 years, establishing Miguel as a force within biofuels.

About the company/institute: Algasol Renewable Po Box no1 07190 Esporles Spain +34971148600 www.algasol.com

Algasol Renewables has naturally transitioned from a technology company, owning the most coveted patent and cost efficient (CAPEX, OPEX and productivity) on algae growth systems towards becoming one of the largest algal biomass manufacturers through its daughter companies, Algasol Bangladesh Ltd and Algae Biomass Bangladesh Ltd.

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Algasol Renewables owns a unique patented PhotoBioReactor (PBR) for low-cost cultivation of micro algae for High Value Products and biofuels. The technology is a flexible (polymer film) PBR floating on water that can be deployed both on land, in ponds, or in the ocean. A worldwide patent covers all key aspects of the PBR technology, by way of density difference. Algasol photobioreactors (PBR’s) produce at least 10 times more protein per area than soybeans and by far more economically. The largest commercial module, i.e. Alga6 covers a hectare for as little as $52,500, (see presentation), that produces well over 100 metric tonnes per year, dry mass, of algae such as Spirulina grown in a sterile environment. Algasol's photobioreactors float on salt-water, avoiding agricultural land use, ILUC. Even floating on the oceans or estuaries Algasol's PBRs require less than 1/10th of the area crops like soybeans require on land. Abstract:

Introduction

While algae are a sustainable source for many end applications, traditional growth systems have, so far, proved to be too expensive and economically impractical to produce algae biomass or economically viable. Two key issues challenge commercialization: 1) the traditional high capital and operational costs of production, and 2) the exploitation of arable land, and the subsequent burden on the global food sector.

Methods

The principles of Algasol’s patented system include:

A) the general concept of controlling the position of a closed photobioreactor by providing a density difference between the fresh/brackish algae culture inside the photobioreactor and the surrounding saltwater,

B) Density Management System (DMS) for submerging/angling the PBR, and

C) Internal aeration system.

The worldwide patented, CAPEX/OPEX technology is a flexible polymer film photobioreactor floating on water that can be deployed on non-arable land, in ponds, or in the ocean. The modular floating PBR technology provides a low-cost design coupled with optimal light exposure, high biomass concentration, low energy consumption and efficient system control with minimum bio fouling,

Algasol's proprietary and patented technology is applied with the use of polymer bags of varying dimensions to optimize the productivity of microalgae at less than 10% of the current productivity systems.

Results

Productivity: >35gr/m2/day Dry Biomass concentration: >10gr/L

CAPEX: Algasol Usd 52,500/ha

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OPEX: A fraction of traditional systems

Conclusion

Algasol's floating patented PBR’s are distinctive in terms of significantly reducing the risk of contamination of the algae culture as well as enabling higher productivity and lower energy use while avoiding the ILUC issue. Compared to the algae fermentation approach, Algasol's PBR is a fraction (<0.30%) of the cost to build and completely eliminates the need for inputs(read sugar) that compete with the global food supply. In short, Algasol's PBR has a 1/10th of the CAPEX of open pond systems while having the benefits of closed photobioreactor with a much higher productivity.

Author: M. Verhein

Presenting author’s e-mail: [email protected]

Affiliations: 1 University of Islas Baleares, Spain 2 Lawrence Berkeley Natinal Lab, CA, USA 3 University of Naples

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ALGAE EUROPE 1-3 December, Lisbon

Press Release 7 September 2015

Spotlight on algae production and commercialization ALGAE EUROPE 2015: Key drivers Products & Consumer trends - Product Development and

Business opportunities - News from Academia

ALGAE EUROPE Kick off

ALGAE EUROPE kicks off on 1-3 December 2015 in the Sana Lisboa Hotel, Lisbon, Portugal. The

yearly joined conference of EABA (European Algae Biomass Association), Directorates General for

Energy and Research & Innovation of the European Commission and DLG BENELUX has a new name.

The organization expects to attract 250 participants for this second edition, as it is an unique opportunity

for the exchange between academia and industry. The conference features a networking based

environment that allows discussion on the evolution of the Algae Biomass sector worldwide and

understand the role of the main European players. Delegates meet on a professional platform for finding

out tomorrow’s solutions today.

Algae future crop

´Algae are the new crop for the future. Algae contain proteins, lipids, carbohydrates, minerals, vitamins

and other elements that can be used as ingredients for a large range of sectors from food & feed to

nutraceutical & cosmeceuticals to biofuels. Algo-biorefineries can maximize the potential of algae

applications and open new opportunities. The valorization of added-value products from algae is setting

the conditions for the development of small or niche markets, which eventually can grow to larger scale´,

says Vítor Verdelho, president of the EABA (European Algae Biomass Association), reacting on the

question why algae are so important.

European Projects

Currently, there are in Europe several projects, from lab scale to demonstration plants and commercial

facilities, aiming to accelerate the commercialization of algal products. The long path from research to

pilot plants and production facilities will be shown in several presentations including some of the most

relevant projects financed by the EC. "The Commission's support for both research and large scale

demonstration projects has stimulated the algae community in the EU and soon we expect to have the

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first commercial algo-biorefineries operating in the EU. Algae can help address several of the EU's policy

objectives for sustainable biofuels and biochemicals."

Unique Platform

ALGAE EUROPE is a unique opportunity to learn and understand the bottlenecks of algae production

and commercialization and interact with the key players. ALGAE EUROPE 2015 offers the opportunity

for all stakeholders to be up-dated on the recent industrial developments in the field, with an overview

of European initiatives. Keynote presentations and panel discussion will provide a high quality forum for

discussion and information exchange. The poster session and the trade show offer even more

possibilities to present innovative products, processes and services.

About ALGAE EUROPE

ALGAE EUROPE kicks off its second edition this year in Lisbon, Portugal. The conference follows the

1st EABA and EC Algae Contractors’ Conference, and the 8th International Algae Congress held in

Florence (Italy) in 2014, which was visited by 188 delegates coming from 34 countries. The Florence

conference had 46 speakers in the program, divided into 11 sessions. There were 3 networking lunches

and a welcome reception.

For more on ALGAE EUROPE 2015 go to www.algaecongress.com.

----- ------ ------ ------- ------ ------ ------ ------- ---------

More information:

EABA - European Algae Biomass Association

President of EABA - Vítor Verdelho Vieira

Telephone: 00351 93 200 601

E-Mail: [email protected]

European Commission Kyriakos Maniatis E-mail: [email protected]

DLG BENELUX

Paulien Hoftijzer Telephone: 0031 65201 1884 E-Mail: [email protected]

Please refer any publications, links or print editions to DLG BENELUX

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During the Seaweed conference, the followingsessions are addressed to explore the potential:-‐ Seaweed harvest and aquaculture -‐ Market, quality and standardization-‐ Seaweeds for humans (food, health and cosmetics)-‐ Seaweeds for animals (feed, health, hygiene)-‐ Seaweeds for plants (ex biostimulants and elicitor)-‐ Green chemistry-‐ Panel: Economics

See you at Seagriculture V in North of Germany!





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