britton chance: the development of in vivo mrs mitchell schnall md, phd
TRANSCRIPT
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Britton Chance: The Development of In Vivo MRS
Mitchell Schnall MD, PhD
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Metabolism as a basis for understanding and curing disease
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Warm Ischemia4 degrees C
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75 second spectrum
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Exercising in the NMR magnet
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The Transfer function: “Furthermore, the slope and extentof the linear portion of Fig. 4 are pivotal in the identification of normal and pathological functions of the limb.”
Figure 4
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Assuming ADP control and Michaelis-Menten kinetics
assuming a constant pH of 7.1, a temperature of 370C, a 1.0-mM magnesium concentration, and a 5-mM ATP concentration giving Kobs, = 132 (1) and Km ADP = 20 AM (19, 24).
Vmax of 52 J/min and a Km of 0.65 ± 0.06.
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PFK
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Recovery kinetics as a Biomarker
PFK deficient PVD patient
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Vitamin K3
Vitamin C
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Detecting Treatment Response
0 5 10
Min
Normal Subject Patient prior to (A) and after (B) therapy
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1985
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BC’s Contribution to MRS• Established feasibility to real time measurement of metabolites in perfused
organs, intact animal models, and humans• Developed a mechanistic approach to interpretation of spectral data for the
purpose of clinical diagnostics• Developed a strategy of using MRS as a pharmaco-dynamic marker of
treatment effect• Established the role of PME (choline in the proton spectrum) as an important
marker of malignancy• Inspired the development of methodology to support further application of
MRS– Surface coils– Chemical shift imaging– Rotating frame imaging– Multinuclear spectroscopy– Hadamard spectroscopy– NMR pulse design methodology– Multimodality Optical-MR spectroscopy
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Brit’s own acknowledgements