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7/29/2019 Brochure NanoWhite

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NanoWhiteTriple activity for skin brightening


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Whitening and Protection

NanoWhite is a liposomal preparation combining

powerul whitening ingredients with antioxidants ora strong brightening eect.

With age, stress and pollution, the skin pigmentationprocess can be disturbed leading to age spots and an

uneven complexion. Thanks to its special composition,

NanoWhite regulates this process through complemen-tary pathways:

Arbutin,whichnaturallyoccursinbearberryleaves,

strongly inhibits tyrosinase activity which is the keyenzyme o the pigment process.

Itssafeprovenactivityisreinforcedbyreduced

glutathione and vitamin C palmitate which haveboth whitening and antioxidant properties.

Theeffectivenessofallthesecomponentsisoptimizedby the liposomal orm.

The whitening eect o NanoWhite has been provenby 3 dierent methods. Results showed that NanoWhite

inhibited both the tyrosinase activity and the activation

o this enzyme. NanoWhite is thus a sae yet powerulwhitening ingredient ideal to reduce and prevent

pigmentation disorders.

Claim Ideas or NanoWhite

Safelyinhibitsthepigmentationprocess*

Reducesmelaninsynthesis*

Foraluminous,evenskintone*invitro

Applications

Whiteningforfaceandbody

Anti-agingwithauniformskintoneBrighteningforaradiantandluminouscomplexion

Formulating with NanoWhite

Recommendeduselevel:25%

Incorporation:Forcoldprocesses,dissolveNanoWhite

intotheaqueousphase.Incold/hotprocesses,addduring the cooling phase below 40 C.

Thermostability:Temperaturesofupto60Covera

short time do not aect the stability o NanoWhite.

INCI / CTFA-Declaration

Lecithin(and)Arbutin(and)LinolenicAcid(and)LinoleicAcid(and)TocopherylAcetate(and)AscorbylPalmitate

(and)Glutathione(and)Alcohol(and)Aqua/Water

NanoWhite

Triple activity for skin brightening

September2012


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Skin Color, Hyper-Pigmentation and Aging

Skincolorlargelydependsonapigmentcalledmelanin.

Itsproductionintheskinisactivatedbysunlightandrequires the presence o a key enzyme called tyrosinase.

Two major orms o melanin exist in humans: eumelanin(brown to black pigment) and pheomelanin (yellow to

red pigment). Each individual has both types o melanin

but in dierent proportions.

Thus, skin color depends on the nature, concentrationand distribution o melanin.

Butfactorssuchasfreeradicals,hormonalchangesand

sun exposure can disturb the pigmentation process.Besides,skinsluminosityanduniformitytendtodecrease

with age. That is the reason why an even-toned skin is

perceived as a sign o youth.

A Combination o Whitening Ingredients

Usedforcenturiesinherbalmedicine,bearberryleaves

contain arbutin which strongly inhibits tyrosinase activity.Arbutinisanaturalderivativeofhydroquinonewhichis

much more stable and saer than hydroquinone itsel

and so can be used regularly or a long period o time.Reducedglutathioneisatripeptidefoundinthehuman

body.Itisoneoftheskinsmostpowerfulnatural

antioxidantsbutitsleveldecreaseswithage.Itinhibitstyrosinase activity due to its reduction potential.

VitaminCpalmitateistheoptimalformofvitaminCfor

topicalapplication.Itcombinesantioxidant,anti-agingand whitening properties and can also lighten the

existing dark spots.

NanoWhite

Whitening with natural arbutin,glutathione and vitamin C


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Enhanced Eciency due to the Liposomal Form

The liposomal orm has 4 main benefts:

Protectionoftheencapsulatedmaterialsagainstoxidation, color change and deactivation

Possibleblendingwithnormallyincompatible

compounds (like water- and oil-soluble components)Increaseofthebioavailabilitythankstothesmallsize

o the liposomes and its high afnity to the stratum

corneumEnhancedandlong-lastingactivity

Mechanism o NanoWhite

MSHNanoWhite

NanoWhite

melanocyte

tyrosinase inactivated

tyrosinase

DOPA

DOPA chrom

DHICA

eumelanin

tyrosine DOPA quinone

cysteinyl DOPA

pheomelanin

cAMP


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NanoWhite

Study results

Eect on Melanin Synthesis

Inastudyconductedonmelanocytes,NanoWhite

wasshowntoinhibitmelaninformation.Speciccells(B-16Melanocytes)werecultivatedwithNDP-MSH,

a stable derivative o the natural hormone -MSHwhich

stimulates skin pigmentation. The melanin content wasdetermined by measurements o the optical density and

thecellviabilitywasevaluatedinparallelbytheMTT

assay.Besides,morphologicalobservationswereper-ormed by microscope.

Resultsshowedthat0.4%NanoWhiteinhibitsmelanin

formationby74%.Besides,thisinhibitionofthepigmentation process is dose-dependent and is not

duetoacytotoxiceffectasshownintheMTTassay.

Visualization o MelanocytesInhibition o Melanin Production

Control1MSH

Control2+MSH

NanoWhite

0.4% 0.13% 0.044%

Control2+MSH

B-16melanocytes,20xmagnied

NanoWhite0.4%

S-187/MibelleBiochemistry S-187/MibelleBiochemistry


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Double Eect on Human Tyrosinase Activity

The NanoWhite mechanism on tyrosinase

activitywasstudiedintwoin-vitrotests.AssayswereperformedbyusingNormalHumanEpidermal

Melanocytes(NHEM)withdifferentconcentrationsof

NanoWhite against controls. The tyrosinase activity wasdetermined by measuring the melanin content (optical

density).

Intherststudy,tyrosinasewasextractedfromNHEM

andincubatedwithNanoWhite.L-DOPA(enzyme

substrate) was then added to start the tyrosinase reaction.Results showed a dose-dependent inhibition o melanin

ormation. NanoWhite can thus directly inhibit tyrosinase

activity.

The second study was perormed on pre-treated

NHEM.Thispre-treatmentconsistedofincubatingNHEMwithNanoWhiteforaspecictime.Theaimwas

to evaluate the eect o NanoWhite on the ormation

and/oractivationoftyrosinase.Afterthisincubationtime, cells were rinsed and lysed in order to release the

tyrosinasewhichmightbeproduced.ThenL-DOPA

(enzyme substrate) was added. Results showed againa dose-dependent inhibiting eect meaning that

NanoWhitecanalsoinhibittheformationand/or

activation o tyrosinase.

Asaconclusion,these2studiesshowthatNanoWhite

can inhibit both the tyrosinase activity and the activationo this enzyme. Thus, NanoWhite can regulate the

tyrosinase activity and prevent pigmentation disorders.

Infuence on Tyrosinase Activation

Inhibitionoftyrosinaseactivityin%

S-195 / Mibelle Biochemistry

0,4 102

Concentration of NanoWhite in %

0

10

20

30

40

50

60

70

80

Inhibitionoftyrosinaseactivationin%

S-195 / Mibelle Biochemistry

0,016 0,40,08

Concentration of NanoWhite in %

0

10

20

30

40

50

60

70

Inhibition o Tyrosinase Activity


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Claim Ideas or NanoWhite

Safelyinhibitsthepigmentationprocess

ReducesmelaninsynthesisForaluminousevenskintone

Applications

Whiteningforfaceandbody

Anti-agingwithauniformskintoneBrighteningforaradiantandluminouscomplexion

Marketing Benets

Targetedactionthankstotheliposomalform

Protectivepropertiesthankstoitshighcontentinantioxidants

Idealforadailyuse

Innovating or your success

MibelleBiochemistrydesignsanddevelopsinnovative,high-qualityactivesbasedon

naturallyderivedcompoundsandprofoundscienticknow-how.Inspiredbynature Realized by science.

Mibe

lle

AG

Bioch

em

istry,

5033Buc

hs

/Sw

itzerlan

d,

Ph

one

+41(0)628361731

,info@m

ibe

lle

bioc

hem

istry.c

om,

www.m

ibe

lle

bio

chem

istry.c

om

NanoWhiteTriple activity for skin brightening

The inormation contained in this publication is provided in good aith and is based on our current knowledge.

No legally binding promise or warranty regarding the suitability o our products or any specifc use is made.

Claimideasareofferedsolelyforyourconsideration,investigationandverication.MibelleBiochemistry willnot

assume any expressed or implied liability in connection with any use o this inormation.

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