camillo golgi nobel prize for the “black stain”. first picture of golgi complex (stained with...
TRANSCRIPT
![Page 1: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/1.jpg)
CAMILLO GOLGI
NOBEL PRIZE FOR THE “BLACK STAIN”
![Page 2: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/2.jpg)
FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN)
CELL BODY OF NEURON
AXON
![Page 3: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/3.jpg)
RAMON Y CAJAL
USED GOLGI’S “BLACK STAIN” TO SHOW NEURONS ARE
INDIVIDUAL CELLS
![Page 4: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/4.jpg)
![Page 5: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/5.jpg)
![Page 6: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/6.jpg)
RAMON Y CAJAL IN HIS LAB
![Page 7: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/7.jpg)
digitalimagingu.com/galleries/digitalvideo/sp...
![Page 8: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/8.jpg)
![Page 9: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/9.jpg)
Wellmann & Heuser '95. Trends cell Biol. 5, 303
![Page 10: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/10.jpg)
http://publications.nigms.nih.gov/insidethecell/chapter1.html
National Institute of General Medical Sciences
By Alisa Zapp Machalek
![Page 11: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/11.jpg)
http://www.nature.com/nature/journal/v441/n7096/extref/nature04717-s16.mov
http://www.nature.com/nature/journal/v441/n7096/extref/nature04717-s16.mov
![Page 12: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/12.jpg)
![Page 13: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/13.jpg)
![Page 14: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/14.jpg)
![Page 15: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/15.jpg)
![Page 16: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/16.jpg)
SCALES
PLASMA MEMBRANE . .. ..... .. RER
TRANSPORT VESICLES
CIS-CISTERNA
TRANS-CISTERNA (aka TGN)
?????
SECRETORY VESICLE
EXOCYTOSIS
SCALE FORMATION IN A PROTOZOAN
THE DEVELOPING SCALES IN THE GOLGI BODY CANNOT FIT INTO
TRANSPORT VESICLES!
![Page 17: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/17.jpg)
J. Cell Sci. 36, 437-459 0979) 437Printed in Great Britain © Company of Biologists Limited 1979
![Page 18: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/18.jpg)
![Page 19: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/19.jpg)
DEVELOPMENT OF SCALES IN Pyranimonas
![Page 20: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/20.jpg)
![Page 21: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/21.jpg)
![Page 22: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/22.jpg)
![Page 23: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/23.jpg)
SCALE FORMATION IN CHRYSOPHYCEAN ALGAE I. Cellulosic and Noncellulosic Wall Components Made by the Golgi ApparatusR. Malcolm Brown, Jr., Werner W. Franke, Hans Kleinig, Heinz Falk, and Peter Sitte
Cell Biol. 1970 May 1; 45(2): 246–271.
Pleurochrysis scherfellii
![Page 24: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/24.jpg)
CHLOROPLAST
WALL MADE OF THIN SCALES
![Page 25: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/25.jpg)
![Page 26: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/26.jpg)
![Page 27: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/27.jpg)
![Page 28: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/28.jpg)
![Page 29: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/29.jpg)
http://publications.nigms.nih.gov/insidethecell/chapter1.html
National Institute of General Medical Sciences
By Alisa Zapp Machalek
![Page 30: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/30.jpg)
(Nature review, Molecular Cell Biology, vol 31, August 2002, 615)
Jennifer Lippincott-Schwartz and Kristien J.M. Zaal
![Page 31: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/31.jpg)
![Page 32: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/32.jpg)
Kristin M. Hager
Assistant Professor Ph.D., University of Alabama-
BirminghamPostdoctoral Fellowship,
University of Pennsylvania School of Medicine
Molecular and Cell Biology of Pathogenic Protozoa
e-mail | labpage
We believe that the protozoan parasite, Toxoplasma gondii, is spectacularly successful due to its ability to secrete proteins that allow it to interact with virtually *any* nucleated host cell during invasion and intracellular survival.
A key step in protein secretion is the organisms' ability to synthesize and properly target these invasion/maintenance proteins to their respective organelles. Our laboratory is interested in dissecting the central steps involved in these phenomena and in general are interested in intracellular trafficking of proteins in protozoan parasites.
![Page 33: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/33.jpg)
![Page 34: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/34.jpg)
![Page 35: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/35.jpg)
1. Ultrastructure of a Toxoplasma gondii tachyzoite. The conoid defines the apical end of the parasite and is thought to be associated with the penetration of the host
cell. Micronemes, rhoptries and dense granules are the three major secretory organelles, found predominately at the apical end of the parasite. Microneme proteins are released very early in the invasion process, facilitating host-cell binding and gliding
motility. Rhoptry proteins are also released during invasion, and can be detected within the lumen and membrane of the newly generated parasitophorous vacuole
(PV). Dense-granule proteins are released during and after the formation of the PV, modifying the PV environment for intracellular survival and replication of the parasite. The apicoplast is a plastid-like four-membrane organelle containing a 35 kb circular
DNA. Most of the proteins functioning within the organelle are encoded by the nucleus, and are specifically targeted to the apicoplast. This targeting involves the
secretory pathway, including the rough endoplasmic reticulum (ER) and a Golgi body situated immediately apical to the nucleus. Targeted proteins have a bipartite N-
terminal extension, consisting of an ER signal sequence followed by a plastid transit peptide. T. gondii cells have a single nucleus and a single mitochondrion. It is hypothesised that reliance on the mitochondrion for cellular metabolism differs
according to the life-cycle stage of the parasite (fig001jac).
![Page 36: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/36.jpg)
![Page 37: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/37.jpg)
![Page 38: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/38.jpg)
![Page 39: CAMILLO GOLGI NOBEL PRIZE FOR THE “BLACK STAIN”. FIRST PICTURE OF GOLGI COMPLEX (STAINED WITH GOLGI’S BLACK STAIN) CELL BODY OF NEURON AXON](https://reader036.vdocuments.net/reader036/viewer/2022062805/5697bfe91a28abf838cb6890/html5/thumbnails/39.jpg)
www.abcam.com/index.html?pageconfig=resource...
Dr Tony Jackson