catalog cromatografie 2015.pdf

7/23/2019 Catalog Cromatografie 2015.pdf

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. . . we Meet your Needs

Columns and suppliescatalog

HPLC GC TLC

SPE and Flash Syringe flters

Vials and caps


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Chromato

graphy

How to contact us

Meeting your needs in all questions concerning ChromatographyTechnical support and customer services Chromatography:

Phone +49 24 21 969-175 or -370

MACHEREY-NAGEL worldwide our products are globally available

Our customers can count on competent and reliable service all over the world:

Branches in France, Switzerland, and the United States with scientically educated sta

Globally operating network of qualied and specially trained distributors in more than 150 coun-tries

For a complete list of branches and authorized distributors see www.mn-net.com/distributors.

Germany and international USA

MACHEREY-NAGEL GmbH & Co. KG

Neumann-Neander-Str. 6852355 Dren

MACHEREY-NAGEL Inc.

2850 Emrick Blvd.Bethlehem, PA 18020

phone +49 24 21 969-0 phone +1 484 821 0984toll-free 0800 2616 000 toll-free 888-321-6224 (MACH)fax +49 24 21 969-199 oder -198 fax +1 484 821 1272e-mail [email protected] E-Mail [email protected]

France Switzerland

MACHEREY-NAGEL EURL

1, rue Gutenberg B.P. 13567722 Hoerdt

MACHEREY-NAGEL AG

Hirsackerstr. 7 Postfach 2554702 Oensingen

phone +33 388 68 22 68 phone +41 62 388 55 00fax +33 388 51 76 88 fax +41 62 388 55 05e-mail [email protected] e-mail [email protected]


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Chromato

graphy

MACHEREY-NAGEL Company prole

Since 1911 MACHEREY-NAGEL has represented high quality, innovation and reliability in chemical andbiomolecular analysis. Friendly expert advice for our highly valued customers as well as outstanding productquality have been the cornerstones of our corporate success for more than 100 years. CEO C. Wagner, the great-granddaughter of the companys founder, has been managing the enterprise since 2000.

Our product ranges:

Filtration Rapid Tests Water Analysis Chromatography Bioanalysis

Dedicated from the very rst:

Milestones of Chromatography at MACHEREY-NAGEL

0 10

160 278 328 370

20 30 40 min

C

1 2

3

4

5

6

7

8

1952:

MN launches the rstproducts for paper

chromatography. In the

same year, Martin andSynge receive the Nobel

Prize in Chemistry forthe development of

partition chromatography.

NUCLEODUR NUCLEOSHELL

1961:

MACHEREY-NAGELbecomes one of the

pioneers in TLC.

1970:

Expansion of theproduct range by columnchromatography

1974:

NUCLEOSIL one of the rstspherical HPLC silicas leads toour core competence in silica

technology

1994:

OPTIMAcapillary columns

for optimum

GC separations

1987:

CHROMABONDcolumns for SPE

2002:NUCLEODUR

high purity

spherical

silica

for HPLC2011:

NUCLEOSHELLcoreshell silica for highest

eciency in HPLC

1982:

rst fused silica capillarycolumns for GC


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Chromato

graphy

MACHEREY-NAGEL on the internet

Visit our Chromatography pages

www.mn-net.com

Product information always up-to-date

ChromaNews

Job opportunities

Material safety data sheets

Free application database with about3000chromatography applications


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Chromato

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Catalog contents

Solid Phase Extraction and Flash chromatographyCHROMABONDphases and accessories for SPE 152

High-throughput SPE and Flash chromatography 5364

Sample claricationCHROMAFILsyringe lters 6676

Vials and closuresVials and closures 77103

Autosampler compatibility 103108

Liquid chromatographyColumns for HPLC 110197

Packings for liquid chromatography 198206

Thin layer chromatographyPlates, kits and adsorbents for TLC 208234

Gas chromatographyCapillary columns for GC 236281

Reagents for GC 282291

Accessories for GC 293297

AppendicesProduct and subject index, index of referencenumbers, trademarks, USP lists

298327


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Samplepre

paration

New products for sample preparation

CHROMABONDHR-Xpert your way to cleaner samples by SPE

The professional concept of innovative RP and mixed-mode ionexchange phases for SPE

CHROMABONDHR-X hydrophobic PS/DVB copolymer CHROMABONDHR-XCand HR-XCWstrong and weak mixed-mode cation exchangers

CHROMABONDHR-XAand HR-XAWstrong and weak mixed-mode anion exchangers

Now available in 2 particle sizes:standard particle size 85 mparticle size 45 mfor smaller sample volumes,lower adsorbent weight andsmaller elution volumes

Order your freesamples [email protected]

Page

10

New CHROMAFILlters for optimum ltration results

CHROMAFILCombi ltersSyringe lters with a coarse glass ber prelterrecommended for solution with a high particle load

New dimensions and pore sizes for Nylon, cellulose acetate and PTFE lters

Page68

Vials and closures completely revised product range

Large selection of

Crimp neck vials, crimp closures, crimping tools Screw neck vials and screw closures Special vials and closures

High quality products at an equitable Page

77

price performance ration

We look forward to sharing our expertise with you:[email protected]


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HPLC

New products for HPLC

NUCLEOSHELLcore-shell silica for rapid, ecient HPLC

Solid core of silicon dioxide,homogeneous shell of porous silica

Highest eciency compared to totally porous materials Pore size 90 ; particle size 2.7 m (core 1.7 m);specic surface 130 m2/g

Lower back pressure enables use onconventional LC systems

Page144

Pressure stability 600 bar

NUCLEOSHELLRP 18 nonpolar high density phase

Key features:

Suitable for LC/MSand HPLC at pH extremes (pH 111) Superior base deactivation, ideal for method development

Recommended application:Overall sophisticated analytical separations,e.g., analgesics, anti-inammatory drugs, antidepressants;herbicides; phytopharmaceuticals; immunosuppressants

Page148

USP L1

O

NH3C

CH3Si(CH3)3

NUCLEOSHELLPFP hydrophobic pentauorophenyl phase

Key features:

Hydrophobic phase with alternative selectivity in comparison to

classical C18modications Separation principle based on 4 retention mechanisms (polar in-

teractions (H bonds), dipole-dipole interactions, - interactions,hydrophobic interactions)

Recommended application:Aromatic and unsaturated compounds, phenols, halogenated com-pounds, isomers, polar compounds like pharmaceuticals, antibiotics;high retention of basic compounds

USP L43

F

F

F

F

F

O

H

O

Page

150

NUCLEOSHELLHILIC zwitterionic phase

NPC IC

HILIC

RPC

Eluent

Analyt

Sorbens

Key features:

Ideal for reproducible and stablechromatography of highly polar analytes

Very short column equilibration times

Page152

Recommended application:Hydrophilic compounds such as organic polar acids and bases, polarnatural compounds, nucleosides, oligonucleotides, amino acids,peptides, water soluble vitamins


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New products for HPLC

NUCLEODURPolarTec RP phase with embedded polar group

Key features:

Excellent base deactivation Suitable for LC/MSand stable in 100 % aqueous mobile phases Pronounced steric selectivity

Recommended application:Exceptional selectivity for phenols and nitrogen containing com-pounds, polar compounds like basic pharmaceuticals, organicacids, pesticides, amino acids, water soluble vitamins, etc.

USP L1and L60

Page

124

NUCLEODURPFP hydrophobic pentauorophenyl phase

Key features:

Hydrophobic phase with alternative selectivity in comparison toclassical C

18modications

Separation principle based on 4 retention mechanisms (polarinteractions (H bonds), dipole-dipole interactions, - interac-tions, hydrophobic interactions)

Suitable for LC/MSdue to low bleeding characteristics

Recommended application:Aromatic and unsaturated compounds, phenols, halogenated com-pounds, isomers, polar compounds like pharmaceuticals, antibiot-ics; high retention of basic compounds

USP L43

Page136

HPLC


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Chromato

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New products for TLC and GC

ALUGRAMXtra optimized TLC silica layers on aluminium

Outstanding wettability for precise colorization results,even with 100 % aqueous derivatization and visualization reagents

Excellent separation eciencyand reproducibility from lot to lot Easy and reliable cuttingdue to an optimized binder system, no aking ofsilica; now available as ALUGRAMXtra SIL Gwith unmodied standard silica ALUGRAMXtra Nano-SILwith unmodied HPTLC silica ALUGRAMXtra SILGURstandard silica with concentrating zone ALUGRAMXtra Nano-SILGURHPTLC with concentrating zone

Pages214218

OPTIMA WAXplusOPTIMAFFAPplus optimized polar phases for GC

WAXplus

Polar phase with improvedcross-linking for reduced col-umn bleeding and better tem-perature stability

H

H

O C

n

OHH

H

H

C

Good solvent stability, extended column life-time, better performance in the MS in com-parison to conventional Wax phases

Recommended application:broad ranges of analyses, e.g., for solventsand alcohols, suitable for aqueous samples

USP G16

Page

261

FFAPplus

Polar polyethylene glycol phase with nitro-terephthalic acid modication and innovativecross-linking

C

O

(OCH2CH2)m O

O2N

C

O

n

Good solvent stability, extended column life-time, better performance in the MS in com-parison to conventional Wax phases,suitable for aqueous samples

Recommended application:FAMEs, allows determination of free carbox-ylic acids without derivatization

USP G35/ close equivalent to G25

Page263


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Chromato

graphy

Notes


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Sample preparation Contents

Solid Phase Extraction (SPE)

Basic principles of SPE 2

CHROMABOND

phases for SPESummary 7

Description of individual phases and products 10

SPE accessories

Vacuum manifolds and accessories 50

Empty columns and accessories 52

High-throughput SPE

Products for automated SPE 53

CHROMABONDMULTI 96 54

Liquid-liquid extraction

CHROMABONDXTR 56

Phase separation

CHROMABONDPTS and PTL 58

Flash chromatography

Glass columns for low pressure ash chromatography 59

CHROMABONDFlash RS cartridges 60

Adsorbents for CHROMABONDFlash cartridges 64

Sample clarication

CHROMAFILsyringe lters 66

CHROMAFILltration cartridges 75

CHROMAFILMULTI 96 lter plates 76

Vials, closures, and accessories

General information on vials and caps 77Vials, closures, and accessories 78

Autosampler compatibility 103

Samplepre

paration


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SolidPhaseExtraction

Solid phase extraction (SPE) is a powerful method forsample preparation and is used by most chromatogra-phers today.

About 25 years ago MACHEREY-NAGEL designed andintroduced CHROMABOND SPE cartridges containing

silica-based adsorbents. Since then we developed thewidest range of phases and products for SPE based onsilica and polymeric materials.

SPE has capabilities in a broad range of applications:

Environmental analyses

Pharmaceutical and biochemical analyses

Organic chemistry

Food analysis

Drinkingwa

ter

U

rine

Food

Soil

Vegetable

Fruit

Table

ts

Blo

od

Was

teoil

PesticidesPAHs PCBs

DrugsDyes

Vitamins

SPE is a form of digital (step-wise) chromatographydesigned to extract, partition, and / or adsorb one ormore components from a liquid phase (sample) ontoa stationary phase (adsorbent or resin). An adsorbedsubstance can be removed from the adsorbent by step-

wise increase of elution strength of the eluent (stepgradient technique). SPE extends a chromatographicsystems lifetime, improves qualitative and quantita-tive analysis, and the demand placed on an analyticalinstrument is considerably lessened.

In general, SPE is used for three important pur-poses in state-of-the-art analyses:

Concentration of the analyte(up to factor 10.000 - increase of chromato-graphic sensibility and improved limits of detec-tion)

Removal of interfering compounds(protection of subsequent analyses like HPLC,GC, TLC, UV or IR spectroscopy, )

Changing an analytes environment to a simplermatrix more suitable for subsequent analyses

Advantages of SPE compared to classical liquid-liquid extraction:

Lower consumption of solvents

Faster enormous time savings

Lower costs per sample

Potential for automation

High consistency in individual sample handling

More specic selectivity because of the broadrange of adsorbents and dierent retentionmechanisms

Optimization of extraction by variation or ad-justing of the solid phase and chromatographicconditions

Separation of food additives

1 2

34

5

Time [min]

1. Acesulfam K2. Saccharin3. Benzoic acid4. Sorbic acid

5. Aspartame

before SPE

after SPE

Basic principles of SPE


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Retention of the analyte

Analyte molecules are enrichedon the adsorbent

Interfering components and

solvent molecules (matrix) arenot retained

Remaining interfering com-ponents are washed from theadsorbent

The analyte is removed fromthe adsorbent by elution with asuitable solvent

3. Washing 4. Elution

Retention of interferingcomponents

Analyte molecules show no

interaction with the adsorbent Interfering components andsolvent molecules (matrix) areretained

Analyte molecules are washedfrom the adsorbent

The solid phase is simply usedto lter the sample

1. Conditioning 2. Sample application

Moisturizing of thesolid phase with thematrix

Sample is pressed ordrawn through thesolid phase

4. Elution

Since analytes can be either adsorbed on the SPE packing material or directly ow through while the interferingsubstances are retained, two general separation procedures are possible both cases are shown in the gureabove.

Main steps of the SPE procedure

1. Conditioning of the adsorbent

Conditioning of the adsorbent is necessary in order toensure reproducible interaction with the analyte. Con-ditioning, also called solvation, results in a wettingof the adsorbent and thus produces an environment,which is suitable for adsorption of the analyte. Non-polar adsorbents are usually conditioned with 23 col-umn volumes of a solvent, which is miscible with water(methanol, THF, 2-propanol etc.), followed by the sol-

vent in which the analyte is dissolved (pure matrix, e.g.,water, buer). Polar adsorbents are conditioned withnonpolar solvents.

After the conditioning step the adsorbent bed must notrun dry, because otherwise solvation is destroyed (de-conditioning).

2. Sample application (adsorption)

Sample application can be performed with positive ornegative pressure with a ow rate of ~3 mL/min. Sam-ple volumes vary from a few mL up to liters.

3. Washing of the adsorbent

Washing of the adsorbent is usually achieved with aspecial wash solution; however, in some cases it maynot be necessary. If the polarity dierence betweenwash solution and eluent is very large, or if both are notmiscible, drying of the adsorbent bed after washing isrecommended to improve elution and recovery.

4. Elution

Elution with a suitable eluent should not be too fast.The elution speed depends on the column or car-tridge dimension and the quantity of adsorbent (about1 mL/min).



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Molecular interactions in SPE

SPE adsorbents are most commonly categorized by thenature of their primary interaction mechanism with theanalyte of interest. The three most common extraction

mechanisms used in SPE are reversed phase (RP), nor-mal phase (NP) and ion exchange.

Typical extraction mechanisms Reversed Phase Extraction of hydrophobic or polar organic analytes from aqueous matrix Normal Phase Extraction of polar analytes from non-polar organic solvents Ion Exchange Extraction of charged analytes from aqueous or non-polar organic samples

Types of retention mechanisms:

Si

Nonpolar interactions

Silica-based: C18ec, C18, C18Hydra, C8Polymer-based: HR-X, HR-P, Easy, PS-RPInteractions: hydrophobicSample: mostly aqueousElution: solvents with lower polarity (compared to water)

CH3OH, CH2Cl2, CHCl3, hexane

N

H

H

OH

SiO

H

OHSi

Polar interactions

Silica-based: SiOH, CN, NH2, OH (diol), C6H5Other: Alox, Florisil

Interactions: hydrogen bonds, dipole-dipole and - interactionsSample: mostly organicElution: polar solvents (compared to sample solvent), e.g.,

(nonprotic) ethers, ketones (MTBE, THF, acetone)CH2Cl2, CHCl3

Si

SO3

R +

NH3

Na+

Na+

Cation exchangers

Silica-based: SA (SCX), PCA (WCX), PSA,Polymer-based: HR-XC, HR-XCW, PS-H+

Interaction: between charged analytes and functional group of cationexchanger

Sample: aqueous (pH 3-5)Elution: acidic: pH 2 (e.g., HCl, or 20 % AcOH in CH3OH CH3CN)

basic: pH 8-9 (e.g., 5 % NH3in CH3OH CH3CN)solvents or buers with higher ionic strength and counterions with high selectivity (e.g., Ca2+)

RNR+ OOC

OH

OH

Si

Anion exchangers

Silica-based: SB (SAX), NH2, DMAPolymer-based: HR-XA, HR-XAW, PS-OH-

Interaction: between charged analytes and functional group of anionexchanger

Sample: aqueous (pH 8-9)Elution: basic: pH 10 (e.g., 20 % NH3in CH3OH CH3CN)

acidic: pH 4-5 (e.g., HCl, or 5 % AcOH in CH3OH CH3CN)solvents or buers with higher ionic strength and counterions with high selectivity (e.g., citrate)

It should be noted, that in SPE the interactions described above are not found in pure form, but in combination. Forexample, modied silicas, unless they have been subjected to endcapping (silanization of residual silanol groupswith short-chain silanes), still possess free silanol groups, which can enter into secondary interactions.



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Sample pretreatment

For direct extraction with adsorbents the sample matrix(sample environment) has to fulll three conditions:

The matrix has to be liquid, if possible with low

viscosity.Solids should be removed from the liquid matrix.

The matrix (sample environment) should be suit-able for retention of the analyte.

For solid samples there are dierent methods to con-vert the sample into a suitable matrix:

Dissolution of the solid sample in a suitable solvent

Lyophilization of the sample and dissolution in asuitable solvent

Extraction of the solid sample with a suitable sol-vent

Homogenization of the sample in a suitable solvent

In order to nd the suitable solvent, one has to con-sider all desired sample components. Also, the suit-able solvent should enhance retention of the analyte.For example, samples with large contents of solids areoften homogenized in nonpolar solvents like hexane,while for samples with high water content dissolutionin acids, bases, buers or very polar solvents such asmethanol is recommended.

Additionally, SPE allows to alter the properties of thesample matrix. If, for example, natural products areextracted with methanol or acetone, the polarity of theextracts can be increased by dilution with water, in or-der to enhance nonpolar solid phase extraction on theC18material.

Our CHROMABONDQC policy Highest production standardour facilities are EN ISO 9001:2008 certied

All of our bonded phases and SPE products are vigorously tested forperfect reproducibilityfrom lot-to-lot and within every single batch

Careful attention to particle size distribution and pore diameters as-sures consistent column ow

Chemical reproducibility is guaranteed by strict quality controlthroughout manufacturing

All products are individually tested to meet our strict quality speci-cations, ensuring our outstanding product reproducibility, reliabilityand performance

Each product is supplied with a certicate of analysisstating theresults of internal examinations and quality control



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CHROMABOND

Sample reservoirpolypropylene or glass

Filterelements

Adsorbent

Luer tip,male

CHROMAFIX

Filterelements

Adsorbent

Luer tip,male

Luer tting, female

CHROMABONDLV

Sample reservoirpolypropylene, 15 mL

Filterelements

Adsorbent

Luer tip,male

CHROMABONDMULTI96

84

90.

1

72.3

Filterelements

Adsorbent

Design of columns, cartridges and 96-well plates

All CHROMABONDcolumns, cartridges and 96-well plates are manufactured from polypropylene (PP) withlowest content of extractables (plasticizers, stabilizers, ) oering blank value free results when using mostcommon solvents. The high quality CHROMABONDadsorbents are kept in place by chemically very inert poly-ethylene lter elements (PE, standard pore size 20 m).

CHROMABONDpolypropylene columns

PP columns with PE lter elements

Dierent sizes from 1, 3, 6 up to 150 mL

Adsorbent weights from 20 mg to 50 g

Male Luer tip as exit

Compatible with most robots (e.g., Gilson ASPEC,Caliper AutoTrace)

CHROMABONDglass columns

Glass columns with chemically very inert glass berlter elements (nominal pore size 1 m)

Two dierent sizes: 3 and 6 mL

Available with all CHROMABONDphases

Excludes any inuence from the column material(e.g., plasticizers)

CHROMAFIXcartridges

PP cartridges with PE lter elements Three dierent sizes with dierent adsorbentweights: Small (0.4 mL), Medium (0.8 mL), Large(1.8 mL)

Female Luer tip at the inlet, male Luer tip as exit

Oers alternative way of handling using positivepressure by syringes or peristaltic pumps

Especially suited for convenient solid phase extrac-tion of small sample volumes

CHROMABONDLV columns

Large volume PP columns with PE lter elements

Three dierent adsorbent weights (100, 200 and500 mg)

Funnel-shaped reservoir with 15 mL volume

Especially for clinical samples - the whole sample(e.g., urine, serum, blood) can be applied to thecolumn in one step

Can be directly used in the Zymatelab robots ofZymark

CHROMABONDMULTI 96 SPE in 96-well format

96-well PP plates with PE lter elements

Cavity volume 1.5 mL

Adsorbent weights 10, 25, 50 and 100 mg

Supplied with any CHROMABONDSPE adsorbents

For simultaneous preparation of 96 samples

Easy method transfer from CHROMABONDcolumns or CHROMAFIXcartridges toCHROMABONDMULTI 96

Readily adaptable to all common automated / ro-botic handling systems (for details see page 54)

Online-SPE (see page 53)

Online columns and cartridges

SPE columns with caps and needles for the GerstelMultiPurposeSampler (MPS)

Columns for Gilson ASPEC systems (ASP)



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For the development kits as well as for all indi-vidual CHROMABOND, CHROMABOND LV andCHROMAFIX types columns are sealed in units ofve columns each to prevent adsorption of contami-nants from the environment, e.g., laboratory air.

Ordering information

Designation Contents of the kit REF

Investigating the best separation mechanism for a clean-up procedureCHROMABONDHR-Xpertdevelopment kit I

columns with 3 mL, 60 mg each (particle size 45 m): 10 columns withHR-X; 5 columns each with HR-XC, HR-XA, HR-XCW, HR-XAW

730723

CHROMABONDHR-Xpertdevelopment kit II

columns with 3 mL, 200 mg each (particle size 85 m): 10 columns withHR-X; 5 columns each with HR-XC, HR-XA, HR-XCW, HR-XAW

730726

CHROMABONDpolymer development kit

5 columns each with 3 mL, 200 mg:HR-X, HR-XC (MCX), HR-XA (MAX), HR-P, Easy, PS-H+, PS-OH

730288

CHROMABONDstandard development kit

5 columns each with 3 mL, 500 mg:C18, C18ec, C8, C6H5, NH2, DMA, OH, CN, SiOH, SA (SCX), SB (SAX)

730496

Selecting the optimum RP phase for a clean-up procedureCHROMABONDRP development kit I 10 columns each with 3 mL, 500 mg: C18, C18ec, C8, C4

and 10 columns each with 3 mL, 200 mg HR-P, HR-X730197

CHROMABONDRP development kit II 10 columns each with 1 mL, 100 mg: C18, C18ec, C8, C4, HR-P, HR-X 730207

CHROMAFIXRP development kit I 10 cartridges each CHROMAFIXS: C18, C18ec, C8, C4, HR-P, HR-X 731883

CHROMABONDRP development kit III 10 columns each with 3 mL, 500 mg: C18, C18ec, C18Hydra, C8and 10 columns each with 3 mL, 200 mg HR-P, HR-X

730490

CHROMABONDRP development kit IV 10 columns each with 1 mL, 100 mg:C18, C18ec, C18Hydra, C8, HR-P, HR-X

730491

CHROMAFIX

RP development kit II 10 cartridges each CHROMAFIX

S: C18, C18ec, C18Hydra, C8, HR-P, HR-X 731886CHROMABONDRP development kit V 10 columns each with 3 mL, 500 mg: C6H5, NO2, C6H11ec, C4, C2 730492

CHROMABONDRP development kit VI 10 columns each with 1 mL, 100 mg: C6H5, NO2, C6H11ec, C4, C2 730493

CHROMAFIXRP development kit III 10 cartridges each CHROMAFIXS: C6H5, NO2, C6H11ec, C4, C2 731887

Selecting the optimum polar phase for a clean-up procedureCHROMABONDpolar development kit I 10 columns each with 3 mL, 500 mg: SiOH, Florisil, NH2, CN, OH 730199

CHROMABONDpolar development kit II 10 columns each with 1 mL, 100 mg: SiOH, Florisil, NH2, CN, OH 730208

CHROMAFIXpolar development kit 10 cartridges each CHROMAFIXS: SiOH, Florisil, NH2, CN, OH 731884

Selecting the optimum ion exchanger for a clean-up procedureCHROMABONDion exchange development kit I

10 columns each with 3 mL, 500 mg: SA (SCX), SB (SAX), HR-XC (MCX),HR-XA (MAX), PS-OH, PS-H+, DMA

730206

CHROMABONDion exchange development kit II

10 columns each with 1 mL, 100 mg: SA (SCX), SB (SAX), HR-XC (MCX),HR-XA (MAX), PS-OH, PS-H+, DMA

730209

CHROMAFIXion exchange development kit I

10 cartridges each CHROMAFIXS: SA (SCX), SB (SAX), HR-XC (MCX),HR-XA (MAX), PS-OH, PS-H+, DMA

731885

CHROMABONDcation exchange development kit I

10 columns each with 3 mL, 500 mg: SA (SCX), PSA, PCA, HR-XC (MCX),HR-XCW (WCX), PS-H+

730494

CHROMAFIXcation exchange development kit

10 cartridges each CHROMAFIXS: SA (SCX), PSA, PCA, HR-XC (MCX),HR-XCW (WCX), PS-H+

731888

Phase selection for clean-up procedures for environmental samplesCHROMABONDkit I for environmentalsample preparation

10 columns each with 3 mL, 200 mg HR-P, 6 mL, 1000 mg C18ec, 6 mL,2000 mg C18PAH, 6 mL, 500/1000 mg CN/SiOH,3 mL, 500/500 mg SA/SiOH

730205

CHROMABONDkit II for environmentalsample preparation

5 columns each with 3 mL, 500/500 mg SiOH-H2SO4/SA, 3 mL, 500 mgSiOH, 6 mL, 1000 mg Florisil, 3 mL, 500/500 mg SA/SiOH, 6 mL,700/2000/700 mg NAN

730349

SPE method development kits


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Code Matrix Modication / Application Similar phases* Page

Reversed phasesHR-X PS/DVB ENVI-Chrom P Strata-X OasisHLB Nexus 14

Easy PS/DVB polar, bifunctional Strata-X OasisHLB Porapak RDX Nexus, Bond

Elut

PPL, Focus Styre Screen

DVB BakerbondH2O-philic DVB IsoluteENV+

20

HR-P PS/DVB Strata SDB-L Bond ElutENV, Bond ElutLMS DCS-PS/DVB, ENV PS-DVB Bakerbond H2O-phobicDVB Isolute101 LiChrolutEN

21

PS-RP PS/DVB removal of organic components like HR-P 22

C18ec silica octadecyl, endcapped Strata C18-E Sep-PaktC18 Bond ElutC18 DSC-18(Lt), ENVI-18, LC-18 CLEAN-UPC18,BakerbondOctadecyl IsoluteC18(EC), LiChrolutRP-18 E

23

C18ec f silica as above, fast ow 23

C18 silica octadecyl, not endcapped Strata C18-U AccuBondC18 BakerbondPolarPlus IsoluteC18 LiChrolutRP-18

24

C18f silica as above, fast ow 24

C18PAH silica special octadecyl phase, for en-richment of PAHs from water

Bakerbond Octadecyl Lightload 42

C18Hydra silica octadecyl, not endcapped, forpolar analytes

25

C8 silica octyl Strata C8 Sep-PakC8 Bond ElutC8 DSC-8,ENVI-8, LC-8 CLEAN-UPC8 AccuBondC8 Bakerbond Octyl IsoluteC8(EC)

26

C4 silica butyl 27

C2 silica dimethyl Bond ElutC2 27

C6H11ec silica cyclohexyl, endcapped Bond ElutCH 28

C6H5 silica phenyl Strata PH Bond ElutPH DSC-Ph CLEAN-UPPhenyl AccuBondPhenyl Bakerbond Phenyl Isolute PH(EC)

29

Normal phases

SiOH silica unmodied Strata Si-1 Bond Elut

silica DSC-Si, LC-Si CLEAN-UPsilica Accubondsilica, Bakerbond silicagel Isolutesilica LiChrolutSi

32

NH2 silica aminopropyl Strata NH2 Sep-PakNH2 Bond Elut NH2 DSC-NH2, LC-NH2 CLEAN-UPaminopropyl AccubondNH2 Bakerbond amino IsoluteNH2 LiChrolutNH2

31

OH (Diol) silica diol DSC-Diol, LC-Diol AccubondDiol (OH) 30

CN silica cyano Strata CN Sep-PakCN Bond ElutCN-U DSC-CN,LC-CN CLEAN-UPCN AccubondCN Bakerbondcyano IsoluteCN LiChrolutCN

30

Alox A aluminium oxide acidic LC-Alumina-A Accubondaluminium oxide A 33

Alox N aluminium oxide neutral LC-Alumina-N Accubondaluminium oxide N 33

Alox B aluminium oxide basic LC-Alumina-B Accubondaluminium oxide B 33

Florisil

magnesium silicate Strata FL-PR Sep-Pak

Florisil

Bond Elut

Florisil

ENVI-Florisil, LC-Florisil CLEAN-UPFlorisilAccubondFlorisil Bakerbond Florisil IsoluteFL LiChrolutFlorisil

34

PA polyamide 6 DPA-6S 34

Ion exchangersSB silica quaternary ammonium anion ex-

changer (SAX)Strata SAX, Sep-PakSAX, Bond ElutSAX DSC-SAX,LC-SAX CLEAN-UPQuaternary Amine AccubondSAX Bakerbond Quaternary Amine IsoluteSAX LiChrolutSAX

37

* Phases which provide a similar selectivity based on chemical or physical properties (list not complete)

Summary of MN phases for SPE


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Code Matrix Modication / Application Similar phases* Page

SA silica benzenesulfonic acid cation exchanger(SCX)

Strata SCX Bond ElutSCX DSC-SCX, LC-SCX CLEAN-UPBenzenesulfonic Acid AccubondSCX Bakerbond Aromatic Sulfonic Acid Iso-luteSCX LiChrolutSCX

36

PCA silica propylcarboxylic acid cation exchanger(WCX) Strata WCX Bond ElutCBA DSC-WCX, LC-WCX CLEAN-UPCarboxylic Acid Bakerbond

Carboxylic Acid IsoluteCBA

35

PSA silica propylsulfonic acid cation exchanger 35

HR-XC PS/DVB strong mixed mode cation exchangerfor basic analytes (MCX)

OasisMCX Strata-X-C HyperSep Retain-CX Styre ScreenDBX

16

HR-XA PS/DVB strong mixed mode anion exchangerfor acidic analytes (MAX)

OasisMAX Strata-X-A HyperSep Retain-AX Styre ScreenQAX

17

HR-XCW PS/DVB weak mixed mode cation exchangerfor basic analytes (WCX)

OasisWCX Strata X-CW 18

HR-XAW PS/DVB weak mixed mode anion exchangerfor acidic analytes (WAX)

OasisWAX Strata X-AW 19

PS-OH PS/DVB strong anion exchanger in OHform 22

PS-H+ PS/DVB strong cation exchanger in H+form 22

PS-Mix PS/DVB mixture of PS-OHand PS-H+ 22PS-Ag+ PS/DVB strong cation exchanger in Ag+form 22

PS-Ba2+ PS/DVB strong cation exchanger in Ba2+form 22

Phases for special applicationsDry Na2SO4 for drying organic samples 47

Drug silica bifunctional C8/SA, for enrichment ofdrugs from urine

Strata Screen-C Bond ElutCertify I DSC-MCAX Clean ScreenDAU AccubondEvidex Bakerbond Narc-2 IsoluteHCX LiChrolutTSC HyperSep Verify CX

38

Drug II silica bifunctional C8/SB, for extraction of THCand derivatives and of acidic analytesfrom biological uids

Strata Screen-A Bond Elut Certify II CleanScreenTHC BakerbondNarc-1 IsoluteHAX HyperSep Verify AX

39

Crosslinks cellulose for enrichment of collagen crosslinks 40

Tetracycline silica special octadecyl phase, for enrichmentof tetracyclines 40

HR-P-AOX PS/DVB for extraction of AOX from water (DIN38409 H22)

41

CN/SiOH silica combination phase for enrichment ofPAHs from soil

44

NH2/C18 silica combination phase for enrichment ofPAHs from water

42

Na2SO4/Florisil combination phase for extraction of hy-drocarbons from water (DIN H-53 / ISODIS 9377-4)

43

SA/SiOH silica combination phase for enrichment of PCBfrom waste oil

Bakerbond PCB-N 45

SiOH-H2SO4/SA

silica combination phase, used together withSiOH for enrichment of PCB from oil

46

NAN silica /AgNO3+Na2SO4

combination phase for enrichment of PCBfrom sludge

44

ABC18 silica octadecyl, with ion exchange functions,for acrylamide analysis

IsoluteM-M 47

Diamino silica primary and secondary amine functions(PSA), for determination of pesticides infood samples (QuEChERS method)

Supelclean PSA, Bond ElutPSA 48

Phase separation CHROMABONDPTL/PTS 58

Liquid-liquid extraction CHROMABONDXTR EXtrelut Chem Elut Hydromatrix 56

* Phases which provide a similar selectivity based on chemical or physical properties (list not complete)

Summary of MN phases for SPE


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The professional concept of innovative SPE phases

The CHROMABONDHR-Xpertfamily comprises 5 polymer-based RP and mixed-mode ionexchange phases:

CHROMABONDHR-X hydrophobic PS/DVB copolymerCHROMABONDHR-XC strong mixed-mode cation exchanger

CHROMABONDHR-XA strong mixed-mode anion exchanger

CHROMABONDHR-XCW weak mixed-mode cation exchanger

CHROMABONDHR-XAW weak mixed-mode anion exchanger

These innovative SPE phases oer

State-of-the-art spherical polymer Two particle sizes (45 m and 85 m) adequate for dierent

sample volumes and matrices Broad spectrum of application with special suitability for enrich-

ment of pharmaceuticals from biological matrices Ideal ow properties due to low content of particulate matter

Optimized pore structureand high specic surface High loadability and outstanding elution properties Low solvent consumption Rapid, economical analyses

High-purity adsorber material Allows highest reproducibility with extremely low blind values Reliable analyses at ultra trace level No method adaptation for new batches necessary

HR-X

conventionalPS/DVB copolymer

0 10 20 30 40 50 60 min

conventional PS/DVB

copolymer

Adsorbent blind values:

CHROMABONDHR-X

The HR-Xpertconcept guarantees:

RP and mixed-mode SPE phases with distinct ion exchange and reversed phase properties:excellent enrichment of neutral, acidic and basic compounds

Modern, spherical support polymer with optimized pore structure and high surface:good reproducibility, reliable and cost-ecient analysis

Possibility for more aggressive washing procedures for matrix removal:

cleaner samples and protection of your HPLC and GC instruments Quantication of analytes also from heavily contaminated samples:lower limits of detection also for critical matrices

CHROMABONDHR-Xpertis the perfect combination for all tasks in sample preparation

CHROMABONDHR-Xpert


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Chemical structures of the phases:

CHROMABONDHR-X

Hydrophobicpolystyrene-divinylbenzene copolymer

spherical base material for efficient enrichmentand ideal flow behavior

CHROMABOND

HR-XCW

CHROMABOND

HR-XA

CHROMABOND

HR-XAW

CHROMABOND

HR-XC

O

OH

N+

OH

N

N

H

SO3

H+

weak acidiccation exchanger

strong basicanion exchanger

weak basicanion exchanger

strong acidiccation exchanger

Similar phases:CHROMABONDHR-X: OasisHLB, Strata-X, Nexus, ENVI-Chrom P

CHROMABONDHR-XC: OasisMCX, Strata-X-C, HyperSep Retain-CX, StyreScreenDBX

CHROMABONDHR-XA: OasisMAX, Strata-X-A, HyperSep Retain-AX, StyreScreenQAX

CHROMABONDHR-XCW: OasisWCX, Strata-X-CW

CHROMABONDHR-XAW: OasisWAX, Strata-X-AW

CHROMABONDHR-Xpert


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2 particle sizes - 1 goal: HR-Xpertfor optimized sample preparationFor dierent application requirements the particle sizes complement each other perfectly.

45 m

Ideal for:

+smaller samplevolumes

+ smalleradsorbent weights

+lower elutionvolumes

85 m

Recommended for:

+ large volume or viscoussamples, heavy matrixload

+operation withoutvacuum possible (e.g.,for volatile analytes)

+ higher adsorbent weightwithout increase in backpressure

Features of 45 m particles

About half the radius results in 8fold particle number per volume for approx. equal adsorbent weight

Same specic surface for both particle sizes: considerably larger freely accessible external surface for 45 m particles

Denser adsorbent packing: enhanced interaction of the analyte with the adsorbent, better extraction results

Ideal elution characteristics

Method:1 mL column with 30 mg CHROMABONDHR-X, 1 mL standard solution (1 mg/mL hexobarbital), dry-ing, elution in portions of 100 L with methanol (see application 305490 at www.mn-net.com/apps)

70

100 200 300 400 500 600

30

40

50

60

0

10

20

Recoveryrate[%]

Elution volume [L]

Recovery rates 45 m Advantages of 45 mparticles:

+faster elution

+lower elution volumes

required

70

100 200 300 400 500 600

30

40

50

60

0

10

20

Recoveryrate[%]

Elution volume [L]

Recovery rates 85 m

Breakthrough behavior in enrichment

Method:1 mL column with 15 mg CHROMABONDHR-X, apply portions of 1 mL standard solution (250 g/mLhexobarbital in water), collect eluates (see application 305480 at www.mn-net.com)45 m (red)The analyte is completely retained up to fraction 10.

85 m (blue)Small amounts even break through with fraction 4.

45 m particles provide better enrichment andbreakthrough behavior for small adsorbent weights.When using larger adsorbent weights this eect isless pronounced, since then analytes have sucientcontact with the 85 m adsorbent particles as well.

0 10 20 30 40 50

0

10

20

30

40

50

60

70

80

90

100

20

15

10

5

0

0 5 10 15 20

85 m45 m

Number of fractions

Breakthrough[%]

Breakthrough[%]

Number of fractions

45 m particles are ideal for small sample and elution volumes, while for large amounts of sample andadsorbent 85 m particles show advantages due to better ow properties.

CHROMABONDHR-Xpert


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The CHROMABONDHR-Xpertconcept

for neutral, acidic and basic analytes

3 paths - 1 goal: cleaner samples

Depending on the character of the analytes HR-Xpertoers suitable adsorbents and optimal methods forsample preparation, cleaning and concentration.

Strong basespKa > 10

CHROMABONDHR-XCW

AcidspKa 28

CHROMABONDHR-XA

Neutralcompounds

CHROMABONDHR-X

Strong acidspKa < 1

CHROMABONDHR-XAW

BasespKa 210

CHROMABONDHR-XC

Method A

1. Samplepretreatment

2. Columnconditioning

3. Sampleapplication

4. Washing 1:(basic)aqueous

5. Washing 2 /Elution 1*:organic

6. Elution 2: or-ganic acidic

Method B




4. Washing:aqueous

5. Elution:organic

Method C




4. Washing 1:(acidic)aqueous

5. Washing 2 /Elution 1*:organic

6. Elution 2:organic basic

Strongbases

Acidiccompounds

Neutralcompounds*

Strongacids

Basiccompounds

* Under organic washing and elution conditions the following compounds will be also eluted:

HR-X: polar compounds such as organic acids and bases

HR-XC, HR-XCW: acidic components and impurities

HR-XA, HR-XAW: basic components and impurities

CHROMABONDHR-Xpert


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HR-X spherical, hydrophobic polystyrene-divinylbenzene adsorbent resin

Hydrophobic polystyrene-divinylbenzene copolymerpH stability 114

High-purity material with highest reproducibility and lowest blankvalues due to an optimized manufacturing process

Spherical particles, size 45 m and 85 m (standard)pore size 5560 ; very high surface 1000 m2/gcapacity 390 mg/g (caeine in water)

Excellent recovery rates especially for the enrichment of pharma-ceuticals and active ingredients due to the spherical structure of theparticles, very homogeneous surface, and optimized pore structure

Recommended application:

Pharmaceuticals / active

ingredients from tablets,creams and water / wastewater

Drugs and pharmaceuticalsfrom urine, blood, serum andplasma

Trace analysis of pesticides,herbicides, phenols, PAHsand PCBs from water

Drugs from water

Column type:CHROMABONDHR-X, 3 mL, 200 mgREF 730931

Sample:1 g/mL each in waterColumn conditioning:5 mL methanol, 5 mL dist. waterSample application:slowly aspirate 500 mL water (pH 3) throughthe columnColumn washing:5 mL waterElution:after drying 3 x 2 mL acetonitrile

Further analysis: HPLC on NUCLEODURC18Gravity, 5 m;see MN Appl. No. 121690

Recovery rate [%]

Compound HR-X Strata X

Ketoprofen 98 92

Ibuprofen 91 93

Pentobarbital 99 95

Meclofenamic acid 92 93

Protriptyline 63 45

Nortriptyline 53 39

MN Appl. No. 304240

Pesticides from water


Sample pretreatment:samples are spiked with 500 ng of eachpesticide in 1000 mL water, adjusted to pH 2 with HCl or pH 7Column conditioning:10 mL methanol, 10 mL dist. waterSample application:slowly pass 1000 mL spiked water sample through the columnwith the aid of a tubing adapter (REF 730243)Elution:after drying 5 mL methanol THF (1:1, v/v)

Further analysis: HPLC

Recovery rates [%]

Compound HR-XpH 2

Compound HR-XpH 7

Metamitron 86 Desisopropylatrazine 90

Quinmerac 90 2,4-Dichlorobenzamide 95Chloridazon 93 Desethylatrazine 89

Picloram 83 Hexazinone 95

Metribuzin 84 Bromacil 103

Cyanazine 83 Simazine 91

Metabenzthiazuron 94 Desethylterbuthylazine 89

Chlortoluron 91 Atrazine 88

Isoproturon 89 Metalaxyl 97

Diuron 91 Metazachlor 93

Dimethenamid-P 89 Propazine 88

Linuron 94 Terbuthylazine 86

Epoxyconazole 85 Metolachlor 97

Penconazole 90

Alachlor 93

Propiconazole-1 89Flufenacet 91

Diufenicam 58

Triallate 42

MN Appl. No. 304250/304260

Options for online-SPE and automated SPE see page 53

Polymer-based reversed phases for SPE


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Highest reproducibility

within each batch from batch to batch

Standard protocol for CHROMABONDHR-X


Sample pretreatment:if necessary, adjust pH value

Column conditioning:5 mL methanolEquilibration:5 mL waterSample application:slowly aspirate the sample through the columnColumn washing:5 mL water methanol (95:5, v/v)Elution:after drying 3 x 2 mL methanol

Further analysis: if necessary, evaporate and redissolve in asuitable solvent; HPLC or GC

MN Appl. No. 304310

Compounds:

AphenobarbitalB pentobarbital

C hexobarbital

0

50

100

A A AB B BC C C

Batch 1 Batch 2 Batch 3

Barbiturates from serum


Sample:100 ng/mL each in serum

Column conditioning:5 mL methanol, 5 mL dist. waterSample application:1 mL spiked serumColumn washing:5 mL waterElution:after drying 3 x 2 mL methanol

Further analysis: HPLC on NUCLEODUR100-5 C18ec, seeMN Appl. No. 117820

MN Appl. No. 304290


Volume Adsorbent weight Pack of

CHROMABONDHR-X polypropylene columns (85 m)30 mg 60 mg 100 mg 200 mg 500 mg 1 g

1 mL 730934 730935 303 mL 730936 730931 730937 30

6 mL 730938 730939 30

15 mL 730940 730941 20

CHROMABONDHR-X polypropylene columns (85 m) BIGpacks200 mg 500 mg

3 mL 730931.250 250

6 mL 730938.250 730939.250 250

CHROMABONDHR-X polypropylene columns (45 m) NEW!30 mg 60 mg 100 mg 200 mg

1 mL 730934P45 730935P45 30

3 mL 730936P45 730931P45 30

CHROMABONDLV-HR-X (85 m)30 mg 60 mg 200 mg

15 mL 732130 732131 732132 30

CHROMABONDMULTI 96 HR-X96 x 10 mg

(45 m)96 x 25 mg

(45 m)96 x 50 mg

(85 m)96 x 100 mg

(85 m)

738530.010M 738530.025M 738530.050M 738530.100M 1

CHROMABONDHR-X adsorbent (85 m)730663 20 g



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HR-XC strong cation exchanger

Strong acidic benzenesulfonic acid cation exchangerexchange capacity 1.0 meq/g, pKa < 1

Base material polystyrene-divinylbenzene copolymerpH stability 114; high purity material, highest reproducibility andlowest blank values due to an optimized production process

Spherical particles, size 45 m and 85 m (standard); pore size 6575 very large specic surface 800 m2/g; pore volume 1.4 cm3/gRP capacity 300 mg/g (caeine in water)

Outstanding recovery rates especially for the enrichment of basic analytes


Basic active ingredients from

heavily matrix-contaminatedsamples like, e.g., urine,plasma, serum

Fungicides from food,melamine from milk

Basic analytes like, e.g.,amines; bases with pKa 210

Fractionation of acidic, neutral and basic

Column type:CHROMABONDHR-XC, 3 mL, 200 mgREF 730952

Sample:1 mL spiked matrix, acidied with 200 L 2 % H3PO4Column conditioning:5 mL methanol, then 5 mL waterSample application:slowly aspirate sample through the columnWashing:2 mL 0.1 mol/L HClElution:2.5 mL methanol (fraction A: neutral and acidic ana-lytes); then 5 mL methanol NH390:10, v/v (fraction B: basicanalytes)

Further analysis for fraction A: HPLC, e.g., on NUCLEODURC18Gravity, see MN Appl. No. 122230; for fraction B: HPLC onNUCLEODURC8Gravity, see MN Appl. No. 118520

Recovery rates [%]Fraction A:neutral and acidicanalytes

Fraction B:basic analytes

Compound HR-XC Compound HR-XC Oasis

MCX

Strata

X-CSuprofen 108 1. Doxepin 101 68 82

Naproxen 85 2. Imipramine 95 71 85

Tolmetin 73 3. Amitriptyline 94 72 78

Phenobarbital 108 4. Trimipramine 92 70 81

Indomethacin 33

Hexobarbital 80 MN Appl. No. 304780

Standard protocol for CHROMABONDHR-XC

Column type:CHROMABONDHR-XC, 3 mL, 200 mgREF 730952

Sample pretreatment:individual sample preparation with refer-ence to analytes and matrixColumn conditioning:5 mL methanolEquilibration:5 mL waterSample application:slowly aspirate sample through the columnWashing 1:2 mL 0.1 mol/L HCl in waterWashing 2 / Elution 1:2 mL methanol (neutral and acidic com-pounds); if necessary, further washing stepsElution 2:after drying 5 mL methanol 5 % NH3(basic com-pounds)


MN Appl. No. 304740



CHROMABONDHR-XC polypropylene columns (85 m)30 mg 60 mg 100 mg 150 mg 200 mg 500 mg

1 mL 730969 730049 303 mL 730956 730952 730953 306 mL 730957 730955 30

CHROMABONDHR-XC polypropylene columns (45 m) NEW!1 mL 730969P45 730049P45 303 mL 730956P45 730952P45 30

CHROMAFIXHR-XC cartridges (85 m)Size

adsorbent weight S

155 mgM

240 mgL

500 mg

731755 731756 731757 30

CHROMABONDHR-XC adsorbent (85 m)730664 100 g

Polymer-based ion exchangers for SPE


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HR-XA strong anion exchanger

Strong basic quaternary ammonium anion exchangerexchange capacity 0.25 meq/g, pKa ~ 18

Base material polystyrene-divinylbenzene copolymerpH stability 114; high purity material with highest reproducibility andlowest blank values due to an optimized production process

Spherical particles, size 45 m and 85 m (standard); pore size 5565 very large specic surface 850 m2/g; pore volume 1.4 cm3/gRP capacity 350 mg/g (caeine in water)

Outstanding recovery rates especially for the enrichment of acidic analytes


Acidic active ingredients

from heavily matrix-con-taminated samples like,e.g., urine, plasma, serum

Phenolic acids,acidic herbicides

Weak/medium-strengthacids with pKa 28

analytes from serum

0

1.000

2.000

3.000

3.500

mAU

0

20

40

60

80

100

0 5 10 15

min

1

23

4

serum sample

without SPE

after SPE with HR-XC

after SPE with HR-XC

Standard protocol for CHROMABONDHR-XA

Column type:CHROMABONDHR-XA, 3 mL, 200 mgREF 730951

Sample pretreatment:individual sample preparation with refer-ence to analytes and matrixConditioning:5 mL methanolEquilibration:5 mL waterSample application:slowly aspirate sample through the columnWashing 1:2 mL 0.1 mol/L NaOH in waterWashing 2 / Elution 1:2 mL methanol (neutral and basic com-pounds), if necessary, further washing stepsElution 2:after drying 5 mL methanol 1 to 10 % formic acid(acidic compounds)

Further analyses: if necessary, evaporate and redissolve in asuitable solvent; HPLC or GC

MN Appl. No. 304970

For further applications onCHROMABONDpolymer phases seeour online application database at

www.mn-net.com/apps



CHROMABONDHR-XA polypropylene columns (85 m)30 mg 60 mg 100 mg 150 mg 200 mg 500 mg

1 mL 730968 730727 303 mL 730950 730951 730954 306 mL 730958 730966 30

CHROMABONDHR-XA polypropylene columns (45 m) NEW!1 mL 730968P45 730727P45 303 mL 730950P45 730951P45 30

CHROMAFIXHR-XA cartridges (85 m)Size

adsorbent weight S

155 mgM

240 mgL

500 mg

731768 731769 731770 50

CHROMABONDHR-XA adsorbent (85 m)730671 100 g



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HR-XCW weak cation exchanger

Weak acidic carboxylic acid cation exchangerexchange capacity >0.7 meq/g, pKa ~ 5

Base material spherical PS/DVB copolymer, pH stability 114high purity material, highest reproducibility andlowest blank values due to an optimized production process

Spherical particles, size 45 m and 85 m (standard); pore size 5060 very large specic surface 850 m2/g; pore volume 1.21.4 cm3/gRP capacity 350 mg/g (caeine in water)

Outstanding recovery rates especially for enrichment of strongly basic analytes


Basic compounds like qua-

ternary amines Active ingredients from

heavily matrix-contami-nated samples like, e.g.,urine, plasma, serum

Strong bases withpKa > 10

Standard protocol for CHROMABONDHR-XCW

Column type:CHROMABONDHR-XCW, 3 mL, 200 mgREF 730739

Sample pretreatment:individual sample preparation with refer-

ence to analytes and matrixColumn conditioning:5 mL methanolEquilibration:5 mL acidied waterSample application:slowly aspirate sample through the columnWashing 1:2 mL acidied waterWashing 2 / Elution 1:2 mL methanol (neutral and acidic com-pounds), if necessary, further washing stepsElution 2:after drying 2 x 2 mL methanol 1 to 5 % formic acid(strongly basic compounds)


MN Appl. No. 305300

Analysis of peruorinated

Column: 125 x 2 mm NUCLEODURSphinx RP, 3 mEluent: A) 10 mmol/L NH4Ac in water methanol (75:25,

v/v); B) 10 mmol/L NH4Ac in acetonitrile methanol(75:25, v/v)

1030 % B in 3 min, 3055 % B in 8 min,5510 % B in 4 min

Flow rate: 0.30 mL/min, temperature 50 CInjection: 2.5 L (5 mg/L each after SPE enrichment)Detection: MS, ESI negative

0

12

3

4

5

6

5 10 min

MN Appl. No. 123340



CHROMABONDHR-XCW polypropylene columns (85 m)30 mg 60 mg 100 mg 150 mg 200 mg 500 mg

1 mL 730731 730733 303 mL 730735 730739 730741 306 mL 730737 730743 30

CHROMABONDHR-XCW polypropylene columns (45 m) NEW!1 mL 730731P45 730733P45 303 mL 730735P45 730739P45 30

CHROMAFIXHR-XCW cartridges (85 m)Size

adsorbent weight S

155 mgM

240 mgL

500 mg

731774 731775 731776 50

CHROMABONDHR-XCW adsorbent (85 m)730674 100 g



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HR-XAW weak anion exchanger

Weak basic secondary and tertiary ammonium anion exchangerexchange capacity >0.5 meq/g, pKa ~ 6

Base material spherical PS/DVB copolymer, pH stability 114high purity material with highest reproducibility andlowest blank values due to an optimized production process

Spherical particles, size 45 m and 85 m (standard); pore size 5565 very large specic surface 850 m2/g; pore volume 1.21.4 cm3/gRP capacity 350 mg/g (caeine in water)

Outstanding recovery rates especially for enrichment of acidic analytes


Peruorinated surfactants

Acidic compounds like sul-fonates

Active ingredients fromheavily matrix-contaminat-ed samples like, e.g., urine,plasma, serum

Strong acids with pKa < 1

surfactants from water

Application in accordance with DIN 38407-42 Column type:

CHROMABONDHR-XAW, 3 mL, 60 mgREF 730747

Sample:500 mL water, spiked with 1 mL standard solution(20 g/L of each compound)Conditioning:2 mL methanol + 5 % ammonia, then 2 mL metha -nol, nally 2 mL waterSample application:slowly aspirate sample through the columnWashing:2 mL water, then 2 mL acetone acetonitrile formicacid (50:50:1, v/v/v), nally 2 mL methanolElution:2 mL methanol with 5 % ammonia

Further analysis: evaporate to dryness in a stream of nitrogenunder slight heating, and redissolve in a suitable solvent forHPLC

Recovery rates [%]:

Compound Recovery

1 Peruoropropionic acid (PFPrA) 103

2 Peruoropentanoic acid (PFPeA) 94

3 Peruorohexanoic acid (PFHxA) 94

4 Peruorooctanoic acid (PFOA) 95

5 Peruorooctane sulfonate K salt (PFOS) 81

6 Peruorododecanoic acid (PFDoDA) 82

MN Appl. No. 305140

impregnated with uorosurfactants?

Standard protocol for CHROMABONDHR-XAW

Column type:CHROMABONDHR-XAW, 3 mL, 200 mgREF 730748

Sample pretreatment:individual sample preparation with refer-ence to analytes and matrixConditioning:5 mL methanolEquilibration:5 mL waterSample application:slowly aspirate sample through the columnWashing 1:25 mmol/L ammonium acetateWashing 2 / Elution 1:2 mL methanol (neutral and basic com-pounds), if necessary, further washing stepsElution 2:after drying 2 x 2 mL methanol 1 to 5 % ammonia(strongly acidic compounds)

Further analyses: if necessary, evaporate and redissolve in asuitable solvent; HPLC or GC

MN Appl. No. 305200



CHROMABONDHR-XAW polypropylene columns (85 m)30 mg 60 mg 100 mg 150 mg 200 mg 500 mg

1 mL 730728 730729 303 mL 730747 730748 730744 306 mL 730749 730745 30

CHROMABONDHR-XAW polypropylene columns (45 m) NEW!1 mL 730728P45 730729P45 303 mL 730747P45 730748P45 30

CHROMAFIXHR-XAW cartridges (85 m)Size

adsorbent weight S

155 mgM

240 mgL

500 mg

731771 731772 731773 50

CHROMABONDHR-XAW adsorbent (85 m)730673 100 g



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Easy polar, bifunctionally modied polystyrene-divinylbenzene copolymer

Polar modied polystyrene-divinylbenzene copolymerwith a weak anion exchanger

Specic surface 650700 m2/g, particle size 80 m,pore size 50 , pH stability 114

The Easy effect:

Without preconditioning Due to bifunctional modication much more hydrophilic

than conventional polystyrene-divinylbenzene polymers Easily wettable with water


polar herbicides and pesticides from water

(acidic, neutral, basic)polar phenols from waterpolyaromatic compoundspolychlorinated biphenylsdrug analysis from urine, blood, serum,plasma, pharmaceuticals and active ingredi-ents from tablets, creams

Recovery of pesticides

Private communication: Mr. Khn, GUB, Waldshut Tiengen, Germany

Column type:

CHROMABONDEasy, 3 mL, 200 mgREF 730754

Column conditioning:1 mL water, 3 mL methanol, 1 mL waterSample application:aspirate the sample through the columnElution:3 x 1 mL acetone

Further analysis:HPLC with NUCLEOSIL120-5 C18

MN Appl. No. 303220

Recovery rates [%]:

Compound Recovery Compound Recovery

Desisopropylatrazine 90 Metalaxyl 962,6-Dichlorobenzamide 93 Isoproturon 94Desethylatrazine 93 Diuron 94Hexazinone 69 Metazachlor 97Terbacil 65 Propazine 95Simazine 81 Terbuthylazine 93Cyanazine 93 Linuron 96Desethylterbuthylazine 91 Metolachlor 97Methabenzthiazuron 94 Triallate 61Chlortoluron 91 Standard 64Atrazine 92



CHROMABONDEasy polypropylene columns30 mg 60 mg 100 mg 200 mg 500 mg 1 g

1 mL 730751 730752 303 mL 730753 730754 730759 306 mL 730755 730756 30

15 mL 730757 730758 20

CHROMABONDEasy polypropylene columns BIGpacks200 mg

3 mL 730754.250 2506 mL 730755.250 250

CHROMABOND

LV-Easy200 mg

15 mL 732472 30

CHROMABONDMULTI 96 Easy96 x 25 mg 96 x 50 mg 96 x 100 mg

738520.025M 738520.050M 738520.100M 1

CHROMABONDEasy adsorbent730661 20 g

Glass columns on request.



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HR-P polystyrene-divinylbenzene adsorbent resin

Highly porous polystyrene-divinylbenzene copolymer

specic surface 1200 m2/g

particle size 50100 m very high binding capacity, up to 30 % of adsorbent

weight (for comparison: silica adsorbents about 3 %)


aromatic compounds

phenols from waternitroaromatics from waterpesticides from waterPAHs from oil

Aromatic amines from water samples

Private communication M. Le, T.C. Schmidt, Department of Chemistry, University Marburg, 1997

Compounds investigated: aromatic amines Column type:

CHROMABONDHR-P, 3 mL, 200 mgREF 730108

Sample pretreatment: adjust to pH 9 using 10 mol/L NaOHColumn conditioning: 2 mL each of methanol, acetonitrile and

105mol/L sodium hydroxide

Sample application:aspirate sample through the column with about 10 mL/minColumn washing:wash with 2 mL dist. water, dry 5 min under vacuumElution: 3 x 1 mL methanol acetonitrile (1:1, v/v)

For recovery rates of numerous aromatic amines please see application 301810 at www.mn-net.com/apps.MN Appl. No. 301810



CHROMABONDHR-P polypropylene columns100 mg 200 mg 500 mg 1 g

1 mL 730280 30

3 mL 730108 730117 30

6 mL 730119 730111 730118 30

CHROMABONDHR-P polypropylene columns BIGpack200 mg

3 mL 730108.250 250

CHROMABONDHR-P glass columns200 mg 500 mg 1 g

3 mL 730108G 30

6 mL 730111G 730118G 30

CHROMABONDLV-HR-P200 mg

15 mL 732108 30

CHROMAFIXHR-P cartridgesSize

Adsorbent weight S

200 mgM

330 mgL

680 mg

731839 731840 731841 50

CHROMABONDMULTI 96 HR-P96 x 100 mg

738111.100M 1

CHROMABONDHR-P adsorbent730615 20 g



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PS-RP / PS-OH/ PS-H+ phases for RP and ion chromatographyPS-Mix / PS-Ag+/ PS-Ba2+

Base material high purity polystyrene-divinylbenzene

copolymers (PS/DVB), pore size 100 , particle size100 m

Very low degree of swelling, thus very well suited forchromatography

Reliable function over the whole pH range from 014

Dierent modications for dierent applications fromelimination of nonpolar compounds up to the removalof specic polar components


Removal of interfering compounds Improves chromatographic separation, if

the interfering components overlap with theanalyte in the chromatogram

Improves lifetime of the chromatographiccolumn, since interfering components canirreversibly block the column packing

Enrichment of the analytes

Properties of the individual modications:

PS-RP hydrophobic PS/DVB copolymer removal of organic interfering components from water

PS-OH strong PS/DVB anion exchanger, OHform

capacity 0.6 meq/g

removal or concentration of anions from water

increasing the pH value in acidic samplesPS-H+ strong PS/DVB cation exchanger, H+form

capacity 2.9 meq/gremoval or concentration of cations from waterdecreasing the pH value of basic samples

PS-Mix mixture of PS-OHand PS-H+ desalting of water

PS-Ag+ strong PS/DVB cation exchanger, Ag+form removal of halide ions from water

PS-Ba2+ strong PS/DVB cation exchanger, Ba2+form removal of sulfate ions from water

Application 301930/302750: removal of halides from aqueous samples shown for the trace analysis ofnitrate besides an excess of chloride or bromide

Compounds investigated:20 ppm nitrate besides2500 ppm chloride or 500 ppm bromide, respectively

Column type:CHROMAFIXPS-Ag+(M)0.8 mL, 480 mg, REF 731865

Column conditioning:1 mL dist. water

Sample application and elution:apply 4 x 1 mL sample fractions to the cartridge, discard 1stmL, collect 2nd,3rdand 4thmL separately

Further analysis: HPLC with column 250 x 4 mm NUCLEOSILAnion II;eluent 2 mmol/L K H phthalate pH 6, 2 mL/min; detection: indirect UV,280 nm (see applications 110440 and 110450 at www.mn-net.com/apps)


Phase Volume / Adsorbent weight Pack of

CHROMABONDPS polypropylene columns3 mL

200 mg3 mL

500 mg6 mL

500 mg6 mL

900 mg

PS-RP 730765 730692 730693 30

PS-OH 730396 730344 730378 30

PS-H+

730690 730376 730377 30PS-Mix 730394 730310 30

CHROMAFIXPS cartridgesSize

S

Adsorbentweight

SizeM

Adsorbentweight

SizeL

Adsorbentweight

PS-RP 731877 200 mg 731875 320 mg 50

PS-OH 731868 200 mg 731860 380 mg 731862 800 mg 50

PS-H+ 731867 230 mg 731861 430 mg 731863 900 mg 50

PS-Mix 731909 230 mg 50

PS-Ag+ 731866 240 mg 731865 480 mg 50

PS-Ba2+ 731871 280 mg 731870 550 mg 50

Polymer-based phases for SPE


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C18ec / C18ec f (f = fast ow) octadecyl silica, endcapped

Base material silica, pore size 60 , particle size45 m for C18ec, 100 m for C18ec f(for fast ow),

specic surface 500 m2

/g, pH stability 28 Octadecyl phases, endcapped, carbon content 14 %

Very nonpolar, hydrophobic interactions with a widevariety of organic compounds

Advantageous for clean-up of samples with largestructural variations (polarity dierences)


nonpolar compounds

aatoxins, amphetamines, antibiotics,antiepileptics, barbiturates, caeine, drugs,preservatives, fatty acids, nicotine, PAHs,pesticides, PCBs, heavy metals, vitamins

very well suited for desalting of samples

C18ec ffor viscous samples



CHROMABONDC18ec polypropylene columns100 mg 200 mg 500 mg 1 g 2 g 5 g 10 g

1 mL 730011 1003 mL 730012 730013 506 mL 730014 730015 730141 30

15 mL 730404 2045 mL 730405 2070 mL 730259 10

CHROMABONDC18ec polypropylene columns BIGpacks500 mg 1 g

3 mL 730013.250 2506 mL 730014.250 730015.250 250

CHROMABONDC18ec glass columns200 mg 500 mg 1 g

3 mL 730012G 730013G 50

6 mL 730014G 730015G 30CHROMABONDLV-C18ec

200 mg 500 mg

15 mL 732012 732013 30

CHROMAFIXC18ec cartridgesSize

Adsorbent weight S

270 mgM

530 mgL

950 mg

731804 731805 731806 50

CHROMABONDMULTI 96 C18ec96 x 25 mg 96 x 50 mg 96 x 100 mg

738011.025M 738011.050M 738011.100M 1CHROMABONDC18ec adsorbent

730611 100 g

CHROMABONDC18ec f polypropylene columns (fast ow)200 mg 500 mg 1 g

3 mL 730269 730018 50

6 mL 730016 730010 30

CHROMABONDC18ec f adsorbent (fast ow)730613 100 g

Silica-based reversed phases for SPE


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C18/ C18f (f = fast ow) octadecyl silica

Base material silica, pore size 60 , particle size 45 m for C18,100 m for C18f(for fast ow), specic surface 500 m2/g,

pH stability 28 Octadecyl phases, not endcapped, carbon content 14 %

Similar to C18ec, however possesses more free silanols (SiOH),which allow secondary interactions with polar groups of the analytes


nonpolar compounds

pesticides C18ffor viscous samples



CHROMABONDC18polypropylene columns100 mg 200 mg 500 mg 1 g 2 g 5 g 10 g

1 mL 730001 100

3 mL 730002 730003 50

6 mL 730004 730005 730130 3015 mL 730028 20

45 mL 730400 20

70 mL 730261 10

CHROMABONDC18polypropylene columns BIGpacks500 mg 1 g

3 mL 730003.250 250

6 mL 730004.250 730005.250 250

CHROMABONDC18glass columns500 mg 1 g

3 mL 730003G 50

6 mL 730004G 730005G 30

CHROMABONDLV-C18200 mg

15 mL 732002 30

CHROMAFIXC18cartridgesSize

Adsorbent weight S

240 mgM

480 mgL

950 mg

731801 731802 731803 50

CHROMABONDMULTI 96 C1896 x 25 mg 96 x 100 mg

738001.025M 738001.100M 1

CHROMABONDC18adsorbent730602 100 g

CHROMABONDC18f polypropylene columns (fast ow)200 mg 500 mg 1 g

3 mL 730402 730008 50

6 mL 730403 730009 30

CHROMABONDC18f adsorbent (fast ow)730612 100 g



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C18Hydra octadecyl silica for polar analytes

Base material silica, pore size 60 ,particle size 45 m, specic surface 500 m2/g,

pH stability 28 Special octadecyl phase for polar analytes, not end-capped, carbon content 15 %


more polar compounds like pesticides and

their polar degradation products,phenols, phenoxycarboxylic acids,nitroaromatics, pharmaceuticals

Pesticides from water

Compounds investigated: triazines and carboxylic amides Column type:

CHROMABONDC18Hydra, 6 mL, 2 gREF 730301

Sample pretreatment: adjust 1000 mL water to pH 78 with diluted NH3and add 100 L of theinternal standards (1 g/L).Column conditioning: 2 x 5 mL methanol, then 2 x 5 mL dist. waterSample application: force or aspirate the sample through the column. Then dry for 2 h with2 bar N

2.

Elution: slowly aspirate 10 mL methanol through the column. Evaporate the eluate to drynessin a tapered ask with a rotation evaporator at 30 C and store in a refrigerator for ~ 15 min.Redissolve the residue in 200 L cold, fresh n-hexane and transfer the solution to a conicHPLC vial (e.g., REF 702891). Store the solution in a refrigerator until chromatography.Recovery rates: between 95 and 100 %

Further analysis: GC with OPTIMA-3 or OPTIMA-6 (e.g., application 250420) or HPLC inaccordance with EN ISO 11369: 1997 on NUCLEOSIL120-3 C18(application 110880)

MN Appl. No. 302060



CHROMABONDC18Hydra polypropylene columns

50 mg 100 mg 200 mg 500 mg 1 g 2 g 3 g1 mL 730294 730295 100

3 mL 730296 730297 730298 50

6 mL 730299 730300 730301 730302 30

CHROMABONDC18Hydra glass columns200 mg 500 mg 1 g

3 mL 730296G 730297G 730298G 50

6 mL 730299G 730300G 30

CHROMABONDLV-C18Hydra200 mg

15 mL 732295 30

CHROMAFIXC18Hydra cartridgesSize

Adsorbent weight S

270 mgM

530 mgL

950 mg

731730 731731 731732 50

CHROMABONDMULTI 96 C18Hydra96 x 100 mg

738294.100M 1

CHROMABONDC18Hydra adsorbent730628 100 g



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C8 octyl silica

Base material silica, pore size 60 , particle size 45 m,specic surface 500 m2/g, pH stability 28

Octyl phase, not endcapped, carbon content 8 %Similar to C18, however slightly more polarsecondary interactions with polar compounds are more pro-nounced due to shorter alkyl chains


pesticides, PCB



CHROMABONDC8polypropylene columns100 mg 200 mg 500 mg 1 g

1 mL 730021 100

3 mL 730022 730023 50

6 mL 730024 730134 30CHROMABONDC8glass columns

500 mg

6 mL 730024G 30

CHROMABONDLV-C8500 mg

15 mL 732023 30


Adsorbent weight

M

520 mg

731808 50

CHROMABONDMULTI 96 C896 x 100 mg

738021.100M 1




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C4 butyl silica


Butyl phase, not endcapped, carbon content 7 %Slightly more polar than C18or C8,due to shorter alkyl chains the silica surface is not completelyshielded


compounds, which are too

strongly retained on C18or C8e.g., analgetics from blood



CHROMABONDC4polypropylene columns100 mg 500 mg

1 mL 730225 100

3 mL 730227 50


Adsorbent weight S

220 mgM

440 mg

731740 731741 50


Glass columns, LV columns and MULTI 96 on request.

C2 dimethyl silica


Dimethyl phase, not endcapped, carbon content 4 %

Similar to C4

Recommended application:e.g., antiepileptics from plasma



CHROMABONDC2polypropylene columns100 mg 500 mg 1 g

1 mL 730169 100

3 mL 730221 50

6 mL 730409 730410 30


Glass columns, LV columns, CHROMAFIXcartridges and MULTI 96 on request.



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C6H11ec cyclohexyl silica, endcapped


Cyclohexyl phase, endcapped, carbon content 9 % Alternative phase for the midpolar range


phenols from water

chloroanilines from wastewateranthelmintics from tissue

Comparison of dierent phases for phenol analysis

Compounds investigated:phenol, 2,4-dinitrophenol, pentachlorophenol

Column types:CHROMABONDC18, 6 mL, 2000 mg, REF 730130CHROMABONDC6H11ec, 6 mL, 2000 mg, REF 730469

Column conditioning:10 mL acetone, 10 mL methanol, and 10 mL dist. water (pH 2)Sample application:aspirate the sample through the column.Elution:10 mL methanol

phenol 2,4-dinitrophenol pentachlorophenolC18 C6H11ec

0

20

40

60

80

100

MN Appl. No. 302150



CHROMABONDC6H11ec polypropylene columns500 mg 1 g

3 mL 730442 50

6 mL 730443 730444 30

CHROMABONDC6H11ec adsorbent730631 100 g


For further applications on CHROMABONDphasessee our online application database at

www.mn-net.com/apps



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C6H5 phenyl silica

Base material silica, pore size 60 , particle size 45 m, specicsurface 500 m2/g, pH stability 28

Phenyl phase, carbon content 8 %Polarity similar to C8

In addition to hydrophobic interactions more selective adsorptionis possible by - interactions due to the electron density of thephenyl ring.


aatoxins

caeinephenols

Flavor compounds from brandy

Compounds investigated: asarone, quinine, coumarin, quassin Column type:

CHROMABONDC6H5, 6 mL, 1000 mgREF 730412

Sample pretreatment:

mix 10 mL sample with 90 mL water and 10 g sodium chloride andadjust to pH 7 with 0.1 mol/L sodium hydroxide solutionColumn conditioning:10 mL methanol, then 10 mL dist. waterSample application:slowly force or aspirate the sample through the columnColumn washing:2.5 mL water, then 2.5 mL pentaneElution:1) 2 x 2.5 mL pentane diethyl ether (7:3, v/v):

asarone, coumarin2) 10 mL 1 mol/L basic methanol diethyl ether (9:1, v/v): quinine3) 5 mL chloroform: quassin

MN Appl. No. 300170



CHROMABONDC6H5polypropylene columns100 mg 200 mg 500 mg

1 mL 730083 1003 mL 730411 730084 50

CHROMABONDC6H5adsorbent730606 100 g




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CN cyanopropyl silica


Cyanopropyl phase, carbon content 5.5 %Polar to midpolar

In addition to weak hydrophobic interactions selective interactionsare possible due to the high electron density of the CN group.


cyclosporins

carbohydrates



CHROMABONDCN polypropylene columns100 mg 200 mg 500 mg

1 mL 730061 100

3 mL 730420 730063 50

6 mL 730421 30CHROMABONDCN adsorbent

730607 100 g


OH (Diol) diol silica


Diol phase, carbon content 5.5 %

PolarProperties similar to SiOH


antibioticsprostaglandins



CHROMABONDOH (Diol) polypropylene columns100 mg 200 mg 500 mg

1 mL 730051 100

3 mL 730417 730053 50

6 mL 730418 30

CHROMABONDOH (Diol) adsorbent

730605 100 g


Silica-based normal phases for SPE


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NH2 aminopropyl silica


Aminopropyl phase, carbon content 3.5 %Polar, weak anion exchanger


trace elements

lipids

Metals: trace elements from water

Compounds investigated: Al, Be, Cu, Cr(VI), Mo(VI), V(V) Column type:

CHROMABONDNH2, 3 mL, 500 mgREF 730033

Sample pretreatment:mix 100 mL water sample with 5 mL 0.001 % alizarinsulfonic acid solution andadjust to pH 5.5 with acetic acid or sodium acetateColumn conditioning:2 column volumes 1 mol/L nitric acid, then 2 column volumes dist. water

Sample application:force or aspirate sample through the column with 34 mL/minColumn washing:2 mL dist. water; dry column under vacuum for 4 minElution:2 column volumes 2 mol/L nitric acid

MN Appl. No. 301910



CHROMABONDNH2polypropylene columns100 mg 200 mg 500 mg 1 g

1 mL 730031

1003 mL 730413 730033 506 mL 730180 730626 30

CHROMABONDNH2polypropylene columns BIGpack500 mg

3 mL 730033.250 250

CHROMABONDNH2glass columns500 mg 1 g

3 mL 730033G 506 mL 730180G 730626G 30

CHROMABONDLV-NH2500 mg

15 mL 732033 30

CHROMAFIXNH2cartridgesSize

Adsorbent weight S

220 mg

731813 50

CHROMABONDMULTI 96 NH296 x 100 mg

738031.100M 1

CHROMABONDNH2adsorbent730603 100 g



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SiOH unmodied silica

Unmodied, weakly acidic silica, pore size 60 , particle size 45 m,specic surface 500 m2/g, pH stability 28

Very polarAdsorbs humidity from air, for this reason it should be kept wellclosed and if necessary dried before use

Due to its high anity for polar compounds it should not be condi-tioned with polar (e.g., methanol) or water-containing solvents.


aatoxins

chloramphenicolpesticidessteroidsvitamins



CHROMABONDSiOH polypropylene columns100 mg 200 mg 500 mg 1 g 2 g 5 g 10 g 50 g

1 mL 730071 100

3 mL 730214 730073 506 mL 730070 730075 730107 30

15 mL 730217 20

45 mL 730406 20

70 mL 730072 10

150 mL 730473 10

CHROMABONDSiOH polypropylene columns BIGpacks500 mg 1 g 2 g

3 mL 730073.250 250

6 mL 730075.250 730107.250 250

CHROMABONDSiOH glass columns200 mg 500 mg 1 g 2 g

3 mL 730214G 730073G 506 mL 730070G 730075G 730107G 30

CHROMABONDLV-SiOH200 mg 500 mg

15 mL 732072 732073 30

CHROMAFIXSiOH cartridgesSize

Adsorb. weight S

230 mgM

420 mgL

880 mg

731828 731829 731830 50

CHROMABONDMULTI 96 SiOH96 x 100 mg

738071.100M 1

CHROMABONDSiOH adsorbent730608 100 g



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Alox A / Alox N / Alox B aluminium oxide, acidic, neutral, basic

Aluminium oxide, high purity, pore volume 0.90 mL/g, particle size 60150 m, specic surface 150 m2/g

Properties of the individual modications:

Alox A: aluminium oxide, acidic pH value 4 0.5

Alox N: aluminium oxide, neutral pH value 7 0.5

Alox B: aluminium oxide, basic pH value 9.5 0.5


Phase Volume Adsorbent weight Pack of

CHROMABONDAlox polypropylene columns500 mg 1 g 4 g

Alox A 3 mL 730452 50

Alox A 6 mL 730453 730017 30

Alox A 45 mL 730455 20Alox N 3 mL 730446 50

Alox N 6 mL 730447 730139 30

Alox N 45 mL 730250 20

Alox B 3 mL 730429 50

Alox B 6 mL 730466 730020 30

Alox B 45 mL 730467 20

CHROMABONDAlox glass columns1 g

Alox N 6 mL 730139G 30

Alox B 6 mL 730020G 30

CHROMABONDLV-Alox

1 gAlox A 15 mL 732210 30

Alox N 15 mL 732091 30

Alox B 15 mL 732205 30

CHROMAFIXAlox cartridgesSize

Adsorb. weight M

850 mgL

1700 mg

Alox N 731844 731845 50

CHROMABONDMULTI 96 Alox96 x 100 mg

Alox A 738253.100M 1

Alox N 738251.100M 1

Alox B 738252.100M 1

CHROMABONDAlox adsorbentsAlox A 730642 100 g

Alox N 730641 100 g

Alox B 730640 100 g

Normal phases for SPE


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Normal phases for SPE

Florisil magnesium silicate

Matrix magnesium silicate (MgO - SiOH 15:85), high purity,particle size 150250 m


organic tin compounds,

aliphatic carboxylic acids,PCBs, PAHs

Ordering informationVolume Adsorbent weight Pack of

CHROMABONDFlorisilpolypropylene columns200 mg 500 mg 1 g 2 g

3 mL 730457 730081 506 mL 730238 730082 730239 30

CHROMABONDFlorisilpolypropylene columns BIGpack1 g

6 mL 730082.250 250

CHROMABONDFlorisilglass columns500 mg 1 g 2 g

6 mL 730238G 730082G 730239G 30

CHROMAFIXFlorisilcartridgesSize

Adsorbent weight L

990 mg

731848 50

CHROMABONDFlorisiladsorbent730622 100 g

LV columns and MULTI 96 on request

PA polyamide 6

Matrix polyamide 6, unmodied, high purity,particle size 4080 m

Recommended application:avonoids, PAHs

Ordering informationVolume Adsorbent weight Pack of

CHROMABONDPA polypropylene columns200 mg 500 mg 1 g

3 mL 730384 730126 50

6 mL 730007 730127 30

CHROMAFIXPA cartridgesSize

Adsorbent weight S

170 mgL

620 mg

731849 731851 50

CHROMABONDPA adsorbent730660 100 g

Glass columns, LV columns and MULTI 96 on request


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Silica-based ion exchangers for SPE

PCA propylcarboxylic acid cation exchanger based on silica


Propylcarboxylic acid modied silica Weakly acidic cation exchanger (WCX)


strong cations



CHROMABONDPCA polypropylene columns500 mg 1 g

3 mL 730482 50

6 mL 730483 730484 30

CHROMABONDLV-PCA500 mg

15 mL 732482 30

CHROMABONDPCA adsorbent730629 100 g

Glass columns, CHROMAFIXcartridges and MULTI 96 on request.

PSA propylsulfonic acid cation exchanger based on silica

Base material silica, pore size 60 , particle size 45 m,

specic surface 500 m2

/g, pH stability 28 Propylsulfonic acid modied silica

Very strong cation exchanger (capacity ~ 0.7 meq/g)

Contrary to the SA phase no - interactions


weak cations



CHROMABONDPSA polypropylene columns100 mg 500 mg 1 g

1 mL 730460 100

3 mL 730462 50

6 mL 730464 30CHROMABONDPSA adsorbent

730630 100 g



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Silica-based ion exchangers for SPE

SA benzenesulfonic acid cation exchanger based on silica (SCX)


Benzenesulfonic acid modied silicastrongly acidic cation exchanger (capacity ~ 0.5 meq/g)adsorbent with hydrophobic and - interactions (benzene ring)

Ion exchange of organic compounds from aqueous matrixelution of interesting compounds with solvent systems, which compen-sate the ionic and nonpolar interactions, e.g., methanolic HCl


amino acids

amineschlorophyllPCB

Sulfonamides in meat and kidney

B. Pacciarelli et al., Mitt. Gebiete Lebensm. Hyg. 82 (1991) 4555Compounds investigated:sulfaguanidine, sulfanilamide, sulfadiazine,sulfathiazole, sulfapyridine, sulfamerazine, sulfamethizole, sulfad-imidine, sulfamethoxypyridazine, sulfachlorpyridazine, sulfadoxine,sulfadimethoxine

Column type:CHROMABONDSA (SCX), 3 mL, 500 mgREF 730077

Sample pretreatment: homogenize 10 g sample and 60 mL dichloro-methane acetone (1:1, v/v) for 30 s with a Polytron. Centrifuge thehomogenate for 10 min at 2500 rpm. Filter the organic phase and washthe lter residue with a little dichlorometh

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