Cell & Tissue damage Research

Innate Immunity · Acute phase proteins · Western blot · TLR · Assays · Antimicrobial peptides · Flow cytometry

· LPS, Microbial toxins · Robust · Complement Research · Antibodies · Easy to use · Scavenger receptors ·

Inflammation · Sensitivity · Adhesion molecules · Unique products · Immunohistochemistry · Coagulation molecules

· Quality control · Cytokines, Chemokines · Cell & Tissue damage · Functional studies · Lipid binding proteins

· Immunoprecipitation · Oxidative stress · Specificity · Organ & Tissue related research · Proteins · Value for money ·

Assays, Human

Name Quantity Cat.#

MPO, Human, ELISA kit 2 x 96 det. HK324H-FABP, Human, ELISA 1 x 96 det. HK401H-FABP, Human, ELISA 2 x 96 det. HK402L-FABP, Human, ELISA * 2 x 96 det. HK404I-FABP, Human, ELISA 2 x 96 det. HK406

* Cross reactivity Sheep, Swine

General introductionHycult Biotech develops, produces and markets antibodies, antibody based products and more specifically immunoassays for innate immunity and directly related fields, with an emphasis on complement, neutrophil proteins, TLR, scavenger receptors and acute phase proteins. Furthermore, we have a strong interest in progressing research in the area of inflammation and cell damage caused by pathogens or oxidation factors or acute organ failure.

Introduction product group Cell & Tissue damageHighly sensitive detection of disturbed cell membrane integrity is of importance in cellular toxicology studies and in various experimental and clinical situations like myocardial infarction and liver transplantation. Small unbound cytosolic proteins show a rapid release from damaged cells. Fatty acid binding proteins (FABP) with tissue specificity are especially useful for early detection of ischemically damaged organs. Interestingly, ischemically damaged organs are characterized histologically by (near) absence of FABP, facilitating recognition of such areas. Aberrant fatty acid metabolism and regulation by fatty acid transporters occurs in diseases like diabetes, obesity, cardiac hypertrophy and failure. Oxidative stress is involved in many human diseases, such as atherosclerosis, Parkinson’s disease and Alzheimer’s disease. In addition, it is assumed to be important in ageing. However, reactive oxygen species can also be beneficial, as they are used by the immune system to attack and kill pathogens.

In this Product Leaflet the following abbreviations for the applications and cross reactivity have been used:

FC: Flow Cytometry

FS: Functional studies

F: Immunohistochemistry Frozen sections

P: Immunohistochemistry Paraffin sections

More information about products within the field of Innate Immunity, Antimicrobial peptides, Microbial toxins, TLR, Inflammation, Cell & Tissue damage, Lipid binding proteins, and Oxidative stress can be found on our website. Please feel free to subscribe to our newsletter to make sure you will be up-to-date of Hycult Biotech’s product information!

Hycult Biotech has worldwide distribution through a network of over 45 distributors. If you have questions regarding pricing and ordering, please contact your local distributor. Distributor information can be found on our website.

We understand technical support is part of the deal when you are interested in or have purchased one of our products. For product specific informa-tion please have a look at our website: www.hycultbiotech.com or contact us: support@hycultbiotech.com.

Why scientists buy our products:· Unique products · Sensitivity · Specificity · Easy to use · Value for money · Two-plates kits · Functional studies · Technical support ·· Standardized assays · Quality control · Inter/intra variation <10% · Well documented products · Broad in applications ·

Assays, Other

Name Quantity Cat.#

MPO, Mouse, ELISA kit 2 x 96 det. HK210MPO, Rat, ELISA kit 2 x 96 det. HK105H-FABP, Mouse, Rat, ELISA * 2 x 96 det. HK403L-FABP, Rat, ELISA ^ 2 x 96 det. HK405L-FABP, Swine, ELISA 2 x 96 det. HK408I-LBP (IL-FABP), Mouse, ELISA # 2 x 96 det. HK409Nitrotyrosine, ELISA kit 2 x 96 det. HK501

* Cross reactivity Horse, Salmon, Swine; ^ Cross reactivity Mouse; # Cross reactivity Rat

Proteins / Peptides

Name Quantity Cat.#

DMPC 5 mg HC4044H-FABP, Human, Recombinant (E.Coli-Derived) 50 µg HC2105I-FABP, Rat, Recombinant (E.Coli-Derived) 50 µg HC3101L-FABP, Rat, Recombinant (E.Coli-Derived) 50 µg HC3102

Oxidized PAPC 1 mg HC4035

Oxidized PAPC 5 mg HC4036PAPC 5 mg HC4043

IA: Immunoassays

IF: Immunofluorescence

IP: Immunoprecipitation

W: Western Blot

Cell & Tissue damage ResearchMonoclonal antibodies, Human

Product name Clone name Quantity Applications Cat. #Apoptotic Neutrophils BOB93 100 µg FC W HM2154Apoptotic Neutrophils, biotinylated BOB93 50 µg FC W HM2154BTApoptotic Neutrophils, FITC BOB93 100 µg FC W HM2154FBeta2-adrenoceptor @ 6H8 100 µg FC FS IA HM2238CD36 FA6-152 100 µg F FC FS IA IP W HM2122CD36, biotinylated FA6-152 50 µg F FC W HM2122BTCD36, FITC FA6-152 100 µg F FC W HM2122FCD68 KP1 1 ml F FC IP P W HM2177CD163 # RM3/1 100 µg F FC IA IP P W HM2157CK-BB + CK-BYK/21E10 100 µg F P W HM2110Endostatin 1837-46 100 µg F FC IA P W HM2188FUS LHC7.15 100 µg IP W IF HM2260H-FABP * 66E2 100 µg F IA IP W HM2016H-FABP, Biotin * 66E2 50 µg F IA IP W HM2017H-FABP 67D3 100 µg F IA IP P W HM2018H-FABP, Biotin 67D3 50 µg F IA P W HM2019L-FABP ^ L2B10 100 µg F IA W HM2049L-FABP, Biotin ^ L2B10 50 µg F IA W HM2050L-FABP K5A6 100 µg F IA W HM2051L-FABP, Biotin K5A6 50 µg F IA W HM2052LOX-1 23C11 100 µg FC HM2138LOX-1, biotinylated 23C11 50 µg FC HM2138BTLOX-1, FITC 23C11 100 µg FC HM2138FM2 muscarinic receptor @ B8E5 100 µg F FS W HM2239Mannose receptor 15-2 100 µg F FC FS IA IP W HM2056Mannose receptor, biotinylated 15-2 50 µg F FC IA W HM2057MARCO $ PLK-1 100 µg F FC FS HM2208MPO 266-6K1 100 µg F IA W HM2164MPO, biotinylated 266-6K1 50 µg F IA W HM2164BTMPO, FITC 266-6K1 100 µg F IA W HM2164F

* Cross reactivity Bovine, Dog, Horse, Mouse, Rat, Salmon, Swine; ^ Cross reactivity Baboon, Dog, Rat, Swine; # Cross reactivity Monkey; $ Cross reactivity Bovine; @ Cross reactivity Guinee pig, Rat; + Cross reacti-vity Mouse, Rabbit, Rat.

Figure 1: Human liver fatty acid binding protein (L-FABP) in the colon epithelial layer of a 9 months old baby. Staining with antibody L2B10 (Cat.# HM2049)

Monoclonal antibodies, Mouse

Product name Clone name Quantity Applications Cat. #CD36 * CRF D2712 200 µg FC FS IP W IF HM1074CD68 * FA-11 100 µg F FC IP W IF HM1070Collagen II ^ CIIC1 100 µg F FS IA P HM1062Collagen II ^ M2139 100 µg F FS IA P HM1063Dectin-1 2A11 100 µg FC FS IP HM1067Mannose receptor MR5D3 100 µg F FC IP W HM1049MARCO ED31 100 µg F FC FS IP HM1068MPO * 8F4 100 µg F FC IA HM1051MPO, biotinylated * 8F4 50 µg F FC IA HM1051BTMPO, FITC * 8F4 100 µg F FC IA HM1051FOSCAR 5B8 100 µg FC FS HM1121SR-A 2F8 100 µg F FC FS IA IP W HM1061TIM-4 Kat5-18 100 µg FC FS IA IF HM1111

* Cross reactivity Rat; ^ Cross reactivity Bovine, Chicken, Human, Rat

Figure 2: MPO in human tonsil tissue. Intravascular staining of paraffin section with polyclonal antibody (Cat.# HP9048)

Figure 3: Myeloperoxidase (MPO) in mouse spleen. Staining of frozen section with antibody 8F4 (Cat. # HM1051)

Monoclonal antibodies, Rat

Product name Clone name Quantity Applications Cat. #CD36 UA009 100 µg F FC FS IA IP HM3019CD68 ED1 100 µg F FC IP P W HM3029CD163 ED2 100 µg F FC IP P HM3025MPO * 2D4 100 µg F FC IA IF HM3030MPO, FITC * 2D4 100 µg F FC IA IF HM3030FMPO, biotinylated * 2D4 50 µg F FC IA IF HM3030BTSR-BI 3D12 100 µg F FC FS HM3027

* Cross reactivity Mouse

P.O. Box 305400 AA UdenThe Netherlands

T +31 413 25 13 35F +31 413 24 83 53E info@hycultbiotech.com

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Cell & Tissue damage Research

Monoclonal antibodies, Other

Product name Clone name Quantity Applications Cat. #BPDE 8E11 100 µg IA HM5008BPDE-DNA 5D11 100 µg F IA P HM5007E-Selectin, Human * ENA1 100 µg F FS IA IP HM4001E-Selectin, Human, biotinylated * ENA1 50 µg F IA HM4002Ethenoadenosine 1G4 100 µg F FC IA IP W HM5005MGO ^ MGO-1 100 µg P W HM5014N-(Carboxymethyl)lysine (CML) ^ CML26 100 µg F P W HM5013Nitrotyrosine HM.11 100 µg F IA P W HM5001Nitrotyrosine, biotinylated HM.11 50 µg F IA P W HM5002Phosphotyrosine P9V9 100 µg IA W HM5003Phosphotyrosine, biotinylated P9V9 50 µg IA W HM5004PrPsc $ 1.5D7 100 µg F P IA W HM5011Ubiquitin Ubi-1 100 µg F IA IP P W IF HM5009

* Cross reactivity Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Monkey; ^ Cross reactivity Multi- species; $ Cross reactivity Hamster, Human, Mouse, Ovine

Polyclonal antibodies

Product name Quantity Applications Cat. #A-FABP, Human * 100 µg IA W HP9028APP, Human + 100 µg IF IP W HP9053ATP7A, Mouse/Rat 100 µg F P IF W HP8040B-FABP, Human 100 µg IA W HP9029Chlorotyrosine 100 µg F IA P HP5002E-FABP, Human 100 µg IA W HP9030IDO, Human 100 µg FC IA P W IF HP5004IDO1, Rat ^ 100 µg F P W HP8036I-FABP, Human # 100 µg IA W HP9020

Product name Quantity Applications Cat. #IL-FABP, Human 100 µg IA W HP9031IL-FABP, Mouse * 100 µg IA P W HP8011L-FABP, Human 100 µg F IP P W HP9021L-FABP, Rat $ 100 µg IA IP P W HP8010M-FABP, Human 100 µg IA W HP9032MPO, Human * ^ 1 ml F IA P HP9048Phosphorylated histone H2AX @ 100 µg W IF HP5001Spi-6, Mouse 100 µg FC W HP8035

Polyclonal antibodies

* Cross reactivity Rat; ^ Cross reactivity Mouse; # Cross reactivity Mouse, Rat, Sheep, Swine; $ Cross reactivity Mouse, Swine; @ Cross reactivity D. melanogaster, Mammalian, X. laevis, Yeast; + Cross reactivity African green Monkey, Human, Mouse, Rat

Figure 4: Myeloperoxidase (MPO) detection in rat neutrophils and monocytes. Staining with antibody 2D4 (Cat. # HM3030)

Figure 5: Benzo[A]pyrenediol-epoxide (BPDE)-DNA in human breast cancer tissue. Staining of frozen section with antibody 5D11 (Cat.# HM5007)