centre for crop and disease management...e-mail: [email protected] . centre for crop and ....
TRANSCRIPT
e-mail: [email protected]
CENTRE FOR CROP AND DISEASE MANAGEMENT
Lars Kamphuis 16/10/2019
Exploiting wild germplasm to expand the genetic diversity and enhance the adaptive potential of domesticated chickpea
@theCCDM
ccdm.com.au
School of Molecular and Life Sciences, Curtin University
@lars_kamphuis
School of Molecular and Life Sciences, Curtin University @theCCDM ccdm.com.au
• Going back to wild germplasm to develop our chickpea crops of the future
von Wettberg et al., 2018
Introducing new genetic diversity
Wild Cicer, a wild mess
School of Molecular and Life Sciences, Curtin University
C. echinospermum
Desi
KabuliC. reticulatum
Two-pronged strategy:
1. Untargeted approach: Identify genetically diverse wild material and introduce diversity into the breeding program
2. Targeted approach: Identify beneficial traits and introduce them into the breeding program
Wild chickpea populations in the field
• Developed 30 F2 populations of wild chickpea x domestic cultivars
• Phenotyped for growth habit, flowering time, time to pod set and vigour in 2018 and 2019
• Post harvest phenotyped for seed shape, colour and size, yield
• F2 populations genotyped using DArTseq
• Made selections with the PBA chickpea breeder in 2018
• 2019 trials in Shenton Park (WA), Mingenew (WA) and Breeza (NSW)
School of Molecular and Life Sciences, Curtin University @theCCDM ccdm.com.au
Screening for disease resistance @ the CCDM
AUDP
C
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
90.00
Leaf
dam
age
(%)
Cicer arietinum Cicer reticulatum Cicer echinospermum
• Identified sources of resistance to Ascochyta blight and sclerotinia stem rot
Ascochyta blight Sclerotinia stem rot
Phd research Virginia Wainaina
School of Molecular and Life Sciences, Curtin University @theCCDM ccdm.com.au
Ascochyta blight resistance in wild germplasm
• Overall C. echinospermum accessions are more resistant than C. reticulatum accessions
• Identified germplasm resistant to multiple ascochyta blight isolates School of Molecular and Life Sciences, Curtin University @theCCDM ccdm.com.au
0
5
10
15
20
25
30
35
40
45
0-0.2 0.2-0.4 0.4-0.6 0.6-0.8 0.8-1.0 1.0-1.2 1.2-1.4 1.4-1.6 1.6-1.8
Num
ber o
f acc
essi
ons
% area damage relative to Kyabra
C. arietinum C. reticulatum C. echinospermum
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
C. echinospermum C. reticulatum
% a
rea
dam
age
rela
tive
to K
yabr
a
Species
*
Future for Chickpeas is bright!
• Sources of disease resistance identified in wild chickpeas
• Broadening the genetic base of Australian chickpeas underway
• Evaluation of material in different environments (WA and NSW) underway
Mingenew Shenton ParkSchool of Molecular and Life Sciences, Curtin University @theCCDM ccdm.com.au
Acknowledgements
The chickpea team (CCDM)Silke JacquesToby Newman Christy GrimeFiona KamphuisYuphin KhentryJared NagyVirginia WainainaRob LeeLars KamphuisRob SymeWinnie Liu
CSIROJens Berger
NSW-DPIKristy Hobson
UWAMaria Pazos-NavarroJudith LichtenzveigJanine Croser
UC DavisDoug CookPeter Chang
Mingenew Irwin GroupMadi George Debbie Gillam
Living FarmJoanne Shaddick
School of Molecular and Life Sciences, Curtin University