cg3476-figures
TRANSCRIPT
Vector
CG4743
CG32103
CG3476
CG4995
colt
SD SG
MME1
Fig. 1
***
C
Vector CG347601234567
Mg
cont
ent (
ng/u
g p r
o te i
n)
b1+b2(mature)500bp
250bp
b1+bI1(precursor)W
T+Vec
tor
WT+C
G3476
A
B
Marker
SD SG
Vector
CG3476
250bp100bp
Fig. 2
A
D
Contro
l
dMME1-R
NAi
rp49p
dMME1
E Control dMME1-RNAi
Fig. 3
F
C
SD SGVector
dMme1-EGFP
B dMme1-EGFP Tom22-mcherry Merge
***
0 10 20 30 40 50 60 700
20
40
60
80
100
NSD NSD-Mg
Time (d)
Surv
ival
rate
s (%
)
0 10 20 30 40 50 60 700
20
40
60
80
100
Time (d)
Surv
ival
rate
s (%
)
Control NSD
Control NSD-Mg
dMME1-RNAi NSD
dMME1-RNAi NSD-Mg
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
2.0
Mg
cont
ent (
ng/u
g pr
otei
n)
0.00
0.02
0.04
0.06
0.08
0.10
0.12
0.14
Zn c
onte
nt (n
g/ug
pro
tein
)
Control NSD
Control NSD-Mg
dMME1-RNAi NSD
dMME1-RNAi NSD-Mg
NS
Control dMME1-RNAi0.00.51.01.52.02.53.03.54.04.5
0.0
0.1
0.2
0.3
0.4
0.5
0.6
Control dMME1-RNAi
Mg
cont
ent (
ng/u
g pr
otei
n)
Zn c
onte
nt (n
g/ug
pro
tein
)***NS
NS NS
C
Fig. 4
Contro
l
dMME1-O
E
rp49
dMME1
BA
D
0 10 20 30 40 50 60 700
20
40
60
80
100
Time (d)
Surv
ival
rate
s (%
)
0 10 20 30 40 50 600
20
40
60
80
100
ND ND+3 mM Mg
Time (d)
Surv
ival
rate
s (%
)
Control dMME1-OE
Control dMME1-OE0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
Control dMME1-OE0.0
0.1
0.2
0.3
0.4
0.5
0.6
Mg
cont
ent (
ng/u
g pr
otei
n)
Zn c
onte
nt (n
g/ug
pro
tein
)
Control ND
Control ND+3mM Mg
dMME1-OE ND
dMME1-OE ND+3mM Mg
0.0
0.1
0.2
0.3
0.4
0.5
0.6
Control ND
Control ND+3mM Mg
dMME1-OE ND
dMME1-OE ND+3mM Mg
0
1
2
3
4
5
Zn c
onte
nt (n
g/ug
pro
tein
)
Mg
cont
ent (
ng/u
g pr
otei
n)
*NS
NS**
NSNS
Control
MME1-OE
dMME1-RNAi
MME1-OE/dMME1-RNAi
*** **
0
20
40
60
80
100
Eclo
sion
rate
s (%
)Fig. 5
Fig. 6
Mg Zn
0
200
400
600
800
1000
Mg
cont
ent (
ng/u
g dr
ied
E. c
oli) Vector
dMme1
**
NS
Vector
Vector
Vector
Vector
Vector
dMme1No IPTG
IPTG
IPTG, 150mM Mg
IPTG, 2mM Zn
IPTG, 150mM Na
100 10-1 10-2 10-3 10-3.5 Dilute
dMme1
dMme1
dMme1
dMme1
Blank dMme1
Vector
dMme1-HIS
A B
C