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    Principles of cheese making

    University of Minnesota

    Origin of cheese

    Good milk gone bad

    - Raw milk l eft at room temperature will sour and

    form a gel that wil l syneresis (whey separation)

    - Can increase syneresis by cutt ing heating andstirring

    -

    curd in a cloth

    Trip across the desert

    - Milk was stored in the stomach of a calf for a trip

    across the desert

    - Enzyme called rennet present in the stomach

    coagulated the mil k and at the end or the trip cheese

    was produced

    Cheese Folk Lore/Trivia

    - Captain Bligh and mut iny on t he HMS Bounty

    - The art of cheese making has been practice since

    several centuries BC in the Mediterranean Basin

    - Largest piece 1964 Worlds fair, Wisconsin Cheddar34,591lbs

    Cheese evolution

    - Cheese has evolved into many varieties with

    various compositi ons, tastes, and shapes

    During most types of cheese manufacture:

    A large port ion of casein and fat in the mi lk i s

    concentrated in the cheese

    Cheese keeps longer than milk during st orage

    (ripening) and its properties (flavor, texture,

    appearance, functi onality) change durin g ripening

    Objective of modern cheese making:

    - Rapidly (manufacture on a time schedule) process

    milk and precisely adjust the composition of thecheese to control quality and yield

    Composition of milk/cheese

    - In its simplest form

    cheese making is a

    process of

    concentrating milk

    Cheese Diversity

    - All cheese manufactu re invol ves col lect ion of mil k,

    cheese making, and ripening

    - Can vary from very simple to com plex modern

    technology

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    Milk transport

    Adapted from Ch eese and Ferment ed Milk Fo ods

    Cheese making

    Adapted from Cheese and Fermen ted Mil k Foods

    Industrial scaleIndustrial scale

    Composition of fresh cheeses

    Adapted from Cheese and Fermen ted Mil k Foods

    Composition of natural ripened cheeses

    Adapted from Ch eese and Ferment ed Milk Foo ds

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    Cheese making steps

    - Complicated process involving numerous steps and

    chemical as well as physical changes

    - Several variable affect yield, composit ion, and qu ality

    of the cheese

    - Optimization of cheese making is i ntricate and the

    process system used influences productio n costs

    - Overall a full understanding of the various physical and

    chemical transformations, their int erdependence, and

    the ways in w hich they can be affected is needed if

    cheese making is going to be based on scientific

    principles instead of rules of thu mb and art

    - Several different discipli nes are required including:

    process engineering, physical chemistr y, biochemistry,

    and microbiology

    Cheese making steps

    Processing steps

    - Pasteurization, culture addition, clotting of milk,

    removal of whey, acid product ion, salting, curd

    fusion, and ripening

    1. Milk pasteurization:

    HTST or LTLT applied to kill pathogen

    2. Addition of culture:

    m croorgan sms

    Also destroys other mi croorg anisms and enzymes

    that may be harmful or helpful during cheese

    ripening

    Usually lactic acid bacteria, specific microorganismsadded are depend on the cheese variety

    Processing steps

    3. Clotting of the milk:

    Enzyme (rennet), acid, or a combination of both

    results in the formation o f a gel

    Caused by casein aggregation and entrapment of fat

    Also plant and mi crobial based enzymes available to

    4. Cutting and removal of whey:

    coagulate milk

    Syneresis of the coagulum formed in st ep 3

    Final curd is 10-30% of the weight of the origi nal milk

    Drier curd is f irmer, resistant to mechanical treatmentand is easier to transport

    Processing steps

    Clotting of the milk:

    Processing steps

    Cutting and removal of whey:

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    Processing steps

    5. Acid product ion:

    Actuall y occurs at all stages of manufacture as a

    result of conversion of l actose into lactic acid by

    lactic acid bacteria (starter culture)

    Changes in the pH of t he curd as a result of acid

    roduction influence curd ro erties flavor and

    6. Salting :

    ripening characteristics

    Salt is added to the cheese (typically 1-4%), salt content

    is often expressed as the ratio of salt:moisture

    Can add salt in various ways but dry salting and brine

    salting are primarily used

    Salt influences texture, flavor, and rip ening

    Processing steps

    7. Curd fusion:

    8. Ripening:

    Can be done by pressing (Cheddar) or melting int o a

    mass (Mozzarella)

    Microbial, biochemical, chemical, and physical processes

    occur and result in changes in flavor, texture, and

    appearance

    - Fresh cheeses are not ripening and may not have

    curd fusion (i.e. cottage cheese)

    Cheese varieties

    - Cheeses can vary widely i n compositi on and can

    be manufactured and cured using a wide variety of

    methods

    - Results: an enormous variety of cheeses are

    available

    consequences?

    Kind of mi lk; st andardization; cheese milk heat treatment;

    pre-acidification of the cheese milk; starter inocul um;

    addition of secondary flora; type of coagulant and

    method of coagulation; cutting; cooking; curd washing;

    curd size and shape; resting; salting; additi ves; pressing;and ripening temp, time, and conditions

    Variations in cheese manufacture

    1. Kind of milk:

    2. Milk s tandardization:

    Cows, goat, sheep, buffalo difference in fatty acid

    profile, fat globule size, and milk pro tein fractions

    ,

    regulations are typically based on moisture content and

    FDB (fat on a dry basis)

    3. Cheese milk heat treatment:

    Influences the natural bacterial flora, lipase activity,

    rennetability of the milk, syneresis, and retention ofprotein in the curd

    Variations in cheese manufacture

    4. Pre-acidi fication of the cheese milk:

    Pre-culture or ripen the milk prior to renneting can

    also add acid to the milk

    Results in a change in the calcium and phosphate

    content and buffering capacity of the cheese

    5. Starter culture inoculum percentage:

    Adjust to obtai n the desi red rate of acid producti on

    Fast acid product ion is desired in most cheese

    Fast acid production shortens the manufacturing time

    Variations in cheese manufacture

    6. Addition of secondary flora:

    Micro flora other than the starter cu lture can be added

    Swiss and other cheese with eyes contain proprionic

    acid bacteria in addition to l actic acid bacteria

    For surface or internal flora development the milk orcheese can be exposed to bacteria (Limburger) or mold

    (blue cheese)

    General the organisms added are mesophilic or

    thermophilic and decompose lactic acid

    They influence pH, flavor, and texture of the cheese via

    proteolysis, lipolysis and formation of flavor

    compounds

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    Variations in cheese manufacture

    7. Type of coagulant and method of addition:

    Rennet or acid (bacteria or direct acidific ation) or a

    combination of rennet and acid

    Many renneting enzymes are available calf, pepsin

    from co ws, pig, chi cken, vegetable (ficin, papain,

    bromelin), molds (mucos mie hei), and bacteria

    They type of coagulant used wi ll influence the gel

    characteristics, syneresis, and ripening characteristics

    of the cheese

    8. Cutting:

    Wire knives of varying size and design or a fixed

    horizontal or vertical rot ating knife can be used

    Syneresis is inf luenced by curd size and stirri ng

    Variations in cheese manufacture

    9. Cooking :

    Cooking time and temperature i nfluence syneresis, and

    rennet activity (proteolysis) duri ng ripening

    Swiss has complete r ennet i nactivation whereas as

    Mozzarella has partial rennet in activation (chymosin is

    more stable at low pH)

    The starter culture is also influence by cooking

    conditions (activity of mesophilic st arter is decreased

    at temps above 30C)

    10. Curd washing:

    As much as 200% water can be added t o decrease t he

    lactose content of the curd influences final cheese

    pH

    Variations in cheese manufacture

    Cooking examples:

    Variations in cheese manufacture

    11. Curd size and cheese shape:

    Final curd size at salting influence salt absorption and

    cooling rate

    Final cheese size and shape infl uences surface area

    and moisture loss during ripening

    arge sca e manu ac ure o e ar c eese u zes

    290 Kg blocks

    12. Resting:

    Time between pressing and salting (Gouda) or time

    between ditch ing and salting (Cheddar)

    Time utilized is variable and depends on the rate ofacid production

    Variations in cheese manufacture

    13. Salting method and salt content:

    Dry salting before pressing (Cheddar and simil ar);

    brine salting after pressing (Mozzarella, Gouda); dry

    salting by rubbing on the surface of the cheese (Feta)

    Salt content varies from .5 to 10% - Feta and Blue are

    Salt content has a major inf luence on flavor, texture,

    and ripening characteristics

    14. Additi ves:

    Spices, etc (caraway, peppers, basil, bacon) are mixed

    with the curd after whey removal

    Variations in cheese manufacture

    15. Pressing :

    Pressing is not utilized for all cheese surface and

    internal mold ri pened cheese is not pressed (mold

    growth requires air)

    Can vary from light pressing to pressing in conj unction

    with a vacuum s stems lar e scale Cheddarproduction)

    Curd can also be pressed under the whey prior to whey

    removal (Gouda, Swiss, and Parmesan)

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    Variations in cheese manufacture

    Pressing:

    Variations in cheese manufacture

    16. Ripening temperature and time:Typically the ripening temp is between 5 and 20C (1

    day to several years)

    A higher t emperature wi ll s peed up the r ipen ingprocess (temperature has a larger effect on lipolysis

    than it does on proteolysis); at high t emperatures

    cheese can sag, oil off, evaporate water, and show

    microbial defects; generally a higher temperature will

    usually leads to cheese defects

    Swiss is the exception it is kept at 23C for 5 wks to

    speed gas production by propionic acid bacteria

    Surface or internal mold ripened (Camembert 85%

    RH; Blue hole pierced in t he cheese; red smear 90-

    95% RH

    How do we look at all these variables?

    - A few main variables are the most cri tical and are

    influenced in combination

    - Concept: Controlled approach to cheese technology

    Objective is the removal of moisture from a coagulum

    Key to cheese manufacture: acid producti on in t he vat

    because the other variable can be control led

    How does acid productio n in the vat influence cheese

    characteristics?

    Moisture expulsion

    Acid production in the vat

    Basic cheese structure is d etermined w hen curd and

    whey is separated (curd mi neral content is crit ical)

    The lowest possible pH is d etermined by the

    residual lactose present and the final salt to

    moisture ratio of the cheese

    Each type of cheese has a characteristic mineral

    content and pH this is the best way to classify

    cheese

    Basic cheese structure and flavor

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    Cheese classification