chp column series - diaion.com · 20×150 20×250 4.6×150 4.6×250 10×150 20×150 20×250...
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Analytical and preparative chromatography columns and materials for pharmaceutical applications5 Analytical and preparative chromatography columns and materials for pharmaceutical applications
MCI GEL™ CHP20/C04
Separation mechanism of CHP series
●Description of MCI GELTM columns and materials
C=ColumnP=Material
Matrix type Particle size
○Polymeric partition chromatography columns and materials MCI GEL™ CHP series
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�M�C�I� �G�E�L�T�M� �s�p�e�c�i�a�l�i�z�e�s� �i�n� �p�o�l�y�m�e�r�-�b�a�s�e�d� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s�.� �T�h�e� �u�s�e� �o�f� �p�o�l�y�m�e�r�-�b�a�s�e�d� �c�o�l�u�m�n�s� �h�a�s� �b�e�c�o�m�e� �m�o�r�e� �w�i�d�e�s�p�r�e�a�d� �t�h�a�n�k�s� �t�o� �t�h�e� �m�a�n�y� �a�d�v�a�n�t�a�g�e�s� �o�f� �t�h�e� �p�o�l�y�m�e�r� �m�a�t�r�i�x� �l�i�k�e� �e�x�c�e�l�l�e�n�t� �s�e�l�e�c�t�i�v�i�t�y�,� �t�h�e� �a�b�s�e�n�c�e� �o�f� �s�p�e�c�i�f�i�c� �a�d�s�o�r�p�t�i�o�n� �w�h�i�c�h� �i�s� �f�o�u�n�d� �c�o�m�m�o�n�l�y� �w�i�t�h� �s�i�l�i�c�a�-�b�a�s�e�d� �p�a�c�k�i�n�g�,� �o�p�e�r�a�b�i�l�i�t�y� �i�n� �a� �w�i�d�e� �p�H� �r�a�n�g�e� �a�n�d� �g�o�o�d� �c�h�e�m�i�c�a�l� �s�t�a�b�i�l�i�t�y� �d�u�e� �t�o� �t�h�e� �i�n�e�r�t� �n�a�t�u�r�e� �o�f� �p�o�l�y�m�e�r�i�c� �m�a�t�e�r�i�a�l�s�.� �T�h�e� �M�C�I� �G�E�L�T�M� �p�a�r�t�i�t�i�o�n� �c�h�r�o�m�a�t�o�g�r�a�p�h�y� �c�o�l�u�m�n�s� �a�r�e� �b�a�s�e�d� �o�n� �a� �p�o�l�y�s�t�y�r�e�n�e� �a�n�d� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�o�r�o�u�s� �p�o�l�y�m�e�r�.� �A�s� �R�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �a�p�p�l�i�e�d� �t�o� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �a� �w�i�d�e� �v�a�r�i�e�t�y� �o�f� �o�r�g�a�n�i�c� �c�o�m�p�o�u�n�d�s�,� �b�o�t�h� �i�n� �t�h�e� �i�s�o�c�r�a�t�i�c� �a�n�d� �g�r�a�d�i�e�n�t� �e�l�u�t�i�o�n� �m�o�d�e�.� �T�h�e� �c�o�m�p�o�u�n�d�s� �i�n�c�l�u�d�e� �p�e�p�t�i�d�e�s�,� �i�n�s�u�l�i�n�,� �s�m�a�l�l� �m�o�l�e�c�u�l�e� �A�P�I�s�,� �n�u�t�r�a�c�e�u�t�i�c�a�l� �c�o�m�p�o�u�n�d�s�,� �w�a�t�e�r�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s� �a�n�d� �n�u�c�l�e�o�t�i�d�e�s�.� �A�s� �N�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �u�s�e�d� �i�n� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �v�a�r�i�o�u�s� �c�a�r�o�t�e�n�o�i�d�s�,� �f�a�t�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s�,� �s�t�e�r�o�i�d�s�,� �a�n�d� �f�o�o�d� �a�d�d�i�t�i�v�e�s�.� �T�h�e�s�e� �c�o�l�u�m�n�s� �t�o�l�e�r�a�t�e� �v�a�r�i�o�u�s� �o�r�g�a�n�i�c� �s�o�l�v�e�n�t�s� �l�i�k�e� �h�e�x�a�n�e�,� �h�e�p�t�a�n�e�,� �m�e�t�h�y�l�e�n�e� �c�h�l�o�r�i�d�e�,� �a�n�d� �a�l�c�o�h�o�l�s�.� �A�s� �N�P� �c�o�l�u�m�n�s�,� �t�h�e�y� �a�r�e� �u�s�e�d� �i�n� �t�h�e� �s�e�p�a�r�a�t�i�o�n� �o�f� �v�a�r�i�o�u�s� �c�a�r�o�t�e�n�o�i�d�s�,� �f�a�t�-�s�o�l�u�b�l�e� �v�i�t�a�m�i�n�s�,� �s�t�e�r�o�i�d�s�,� �a�n�d� �f�o�o�d� �a�d�d�i�t�i�v�e�s�.� �V�a�r�i�o�u�s� �o�r�g�a�n�i�c� �s�o�l�v�e�n�t�s� �l�i�k�e� �H�e�x�a�n�e� �H�e�p�t�a�n�e�,� �m�e�t�h�y�l�e�n�e� �c�h�l�o�r�i�d�e� �a�n�d� �a�l�c�o�h�o�l�s� �c�a�n� �b�e� �u�s�e�d�.
�T�h�e� �M�C�I� �G�E�L�T�M� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �a�r�e� �b�a�s�e�d� �o�n� �t�h�e� �s�a�m�e� �c�h�e�m�i�s�t�r�i�e�s� �o�f�f�e�r�e�d� �i�n� �t�h�e� �D�i�a�i�o�n�T�M� �a�n�d� �S�e�p�a�b�e�a�d�s�T�M� �s�y�n�t�h�e�t�i�c� �a�d�s�o�r�b�e�n�t� �r�e�s�i�n�s�.� �T�h�e�s�e� �p�o�l�y�m�e�r� �c�h�e�m�i�s�t�r�i�e�s�,� �l�i�k�e� �D�i�a�i�o�n�T�M� �H�P� �s�e�r�i�e�s� �a�n�d� �S�e�p�a�b�e�a�d�s�™� �S�P� �s�e�r�i�e�s�,� �a�r�e� �w�i�d�e�l�y� �u�s�e�d� �a�n�d� �d�o�c�u�m�e�n�t�e�d� �i�n� �t�h�e� �b�i�o�p�h�a�r�m�a�c�e�u�t�i�c�a�l� �i�n�d�u�s�t�r�y� �f�o�r� �f�e�r�m�e�n�t�a�t�i�o�n� �e�x�t�r�a�c�t�i�o�n�,� �t�h�e� �f�o�o�d� �i�n�d�u�s�t�r�y� �a�n�d� �i�n� �i�n�d�u�s�t�r�i�a�l� �c�h�r�o�m�a�t�o�g�r�a�p�h�i�c� �s�e�p�a�r�a�t�i�o�n�s�.� �T�h�e� �M�C�I� �G�E�L�T�M� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �a�r�e� �a�v�a�i�l�a�b�l�e� �a�s� �p�a�c�k�e�d� �c�o�l�u�m�n�s� �f�o�r� �a�n�a�l�y�t�i�c�a�l� �a�p�p�l�i�c�a�t�i�o�n�s�,� �a�n�d� �a�s� �b�u�l�k� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �f�o�r� �a�n�a�l�y�t�i�c�a�l�,� �p�r�e�p�a�r�a�t�i�v�e� �a�n�d� �p�r�o�d�u�c�t�i�o�n� �c�h�r�o�m�a�t�o�g�r�a�p�h�y� �a�p�p�l�i�c�a�t�i�o�n�s�.
5 4.6×150CHK45/C05
*CHP20/C04, CHP20/C10: USP classification is L21
Br
None
Cation exchange group
None
C18
4
10
4.6×15020×150
4
10
4.6×15020×200
4.6×1504.6×25010×25020×15020×250
4.6×1504.6×25010×15020×15020×250
CHP20/C04
44.6×15020×200CHPOD/C04
CHP20/C10
CHP07/C04
CHP07/C10
4 4.6×150CHK40/C04
4
10
4.6×15020×150
4.6×1504.6×25010×25020×15020×250
CMG20/C04
CMG20/C10
Weak cationexchange group
Column list●CHP column series
5
�M�C�I� �G�E�L�T�M� �C�H�P� �s�e�r�i�e�s� �a�r�e� �s�u�i�t�a�b�l�e� �f�o�r� �R�P� �a�n�d� �N�P� �c�h�r�o�m�a�t�o�g�r�a�p�h�y�.� �T�h�e�r�e� �a�r�e� �f�o�u�r� �k�i�n�d�s� �o�f� �c�o�l�u�m�n�s� �o�f� �v�a�r�i�o�u�s� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s�;� �p�o�r�o�u�s� �p�o�l�y�s�t�y�r�e�n�e�,� �m�o�d�i�f�i�e�d� �p�o�r�o�u�s� �p�o�l�y�s�t�y�r�e�n�e�,� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e�,� �a�n�d� �m�o�d�i�f�i�e�d� �p�o�r�o�u�s� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e�.� �T�h�i�s� �r�a�n�g�e� �o�f� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l�s� �o�f�f�e�r�s� �t�r�e�m�e�n�d�o�u�s� �s�c�o�p�e� �f�o�r� �a� �p�r�o�p�e�r� �s�e�l�e�c�t�i�o�n� �o�f� �c�o�l�u�m�n�s� �b�a�s�e�d� �o�n� �t�h�e� �p�r�o�p�e�r�t�i�e�s� �o�f� �t�h�e� �t�a�r�g�e�t� �c�o�m�p�o�u�n�d�s�.
Full range
Column size[mm I.D.×mm]
Particle size[μm] pH range
�P�o�l�y�s�t�y�r�e�n�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�2�0�/�C�0�4�,� �C�H�P�2�0�/�C�1�0�M�o�d�i�f�i�e�d� �p�o�l�y�s�t�y�r�e�n�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�0�7�/�C�0�4�,� �C�H�P�0�7�/�C�1�0�,� �C�H�K�4�0�/�C�0�4�P�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�M�G�2�0�/�C�1�0�M�o�d�i�f�i�e�d� �p�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �p�a�c�k�i�n�g�:� �M�C�I� �G�E�L�T�M� � �C�H�P�O�D�/�C�0�4�,� �C�H�K�4�5�/�C�0�5
MCI GEL™ CHP07/C04, C10 > CHP20/C04, C10 > CHPOD/C04 ≥ ODS columns ≥ CMG20/C04, C10�P�o�l�y�m�e�r� �c�o�l�u�m�n�s� �f�o�r� �H�P�L�C�,� �w�i�t�h� �t�h�e�i�r� �s�u�p�e�r�i�o�r� �c�h�e�m�i�c�a�l� �r�e�s�i�s�t�a�n�c�e�,� �c�a�n� �b�e� �u�s�e�d� �w�i�t�h� �v�a�r�i�o�u�s� �m�o�b�i�l�e� �p�h�a�s�e�s� �o�f� �b�r�o�a�d� �p�H� �r�a�n�g�e�,� �a�c�i�d�i�c� �t�h�r�o�u�g�h� �a�l�k�a�l�i�n�e�.� �T�h�e�y� �h�a�v�e� �t�h�e� �f�o�l�l�o�w�i�n�g� �a�d�v�a�n�t�a�g�e�s� �d�u�e� �t�o� �t�h�e�i�r� �h�i�g�h� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s�:�1)�I�n� �r�e�v�e�r�s�e� �p�h�a�s�e� �c�h�r�o�m�a�t�o�g�r�a�p�h�i�c� �m�e�t�h�o�d�s� �t�o� �s�e�p�a�r�a�t�e� �a�c�i�d�i�c� �o�r� �a�l�k�a�l�i�n�e� �c�o�m�p�o�u�n�d�s�,� �e�l�u�e�n�t�s� �t�h�a�t� �c�a�n� �s�u�p�p�r�e�s�s� �t�h�e�
ionic properties of such compounds are generally used. Polymer columns can be applied in these cases where ODS �c�o�l�u�m�n�s� �w�o�u�l�d� �b�e� �u�n�s�u�i�t�a�b�l�e�.
�2)Some extremely hydrophilic compounds, e.g., oligosaccharides, can be separated using strongly hydrophobic �C�H�P�0�7�/�C�0�4� �o�r� �C�H�P�0�7�/�C�1�0� �c�o�l�u�m�n�s�.
�3)� �P�o�l�y�m�e�r� �c�o�l�u�m�n�s� �c�a�n� �b�e� �w�a�s�h�e�d� �w�i�t�h� �a�c�i�d�i�c� �a�n�d�/�o�r� �b�a�s�i�c� �s�o�l�u�t�i�o�n�s� �i�n� �c�a�s�e� �o�f� �c�o�n�t�a�m�i�n�a�t�i�o�n�.�P�o�l�y�m�e�t�h�a�c�r�y�l�a�t�e� �c�o�l�u�m�n�s�,� �C�M�G�2�0�/�C�0�4� �a�n�d� �C�M�G�2�0�/�C�1�0�,� �c�a�n� �b�e� �a�p�p�l�i�e�d� �b�o�t�h� �f�o�r� �r�e�v�e�r�s�e� �p�h�a�s�e� �a�n�d� �n�o�r�m�a�l� �p�h�a�s�e� �c�h�r�o�m�a�t�o�g�r�a�p�h�y�.Modified polystyrene packing, CHK40/C04, is a mixed-mode type material; both hydrophobic and hydrophilic �i�n�t�e�r�a�c�t�i�o�n�s� �o�c�c�u�r� �b�e�t�w�e�e�n� �t�h�e� �p�a�c�k�i�n�g� �m�a�t�e�r�i�a�l� �s�u�r�f�a�c�e� �a�n�d� �t�h�e� �a�n�a�l�y�t�e�s�.� �T�h�i�s� �m�a�t�e�r�i�a�l� �i�s� �u�s�e�f�u�l� �f�o�r� �c�o�m�p�o�u�n�d�s� �t�h�a�t� are difficult to separate using existing ODS or other polymer-based columns. This column is also used in the normal �p�h�a�s�e� �m�o�d�e� �a�n�d� �s�h�o�w�s� �a� �u�n�i�q�u�e� �s�e�p�a�r�a�t�i�o�n� �p�r�o�f�i�l�e�.� All polymeric columns exhibit superior stability and yield in comparison to ODS columns, which may have free silanol groups even when end-capping agents have been used.
�T�h�e� �h�y�d�r�o�p�h�o�b�i�c�i�t�i�e�s� �o�f� �t�h�e� �c�o�l�u�m�n�s� �a�r�e� �i�n� �t�h�e� �f�o�l�l�o�w�i�n�g� �o�r�d�e�r�s�:
Product nameMatrix Type Functinal group USP
2~12
L21
Analytical and preparative chromatography columns and materials for pharmaceutical applicationsCHP column series
Styrene Divinylbenzene
Methacrylates
Analytical and preparative chromatography columns and materials for pharmaceutical applications
2
3
4
5
6
8
9
10
1
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9
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10 10
1
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6
8
7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
2
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5
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7
8
9
1
2
3
5
6
7
8
9
1
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4
6
7
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1
2
3
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5
7
8
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9 9
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10 10 10 10 10 10 10 10
1
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9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
33 34
Durability of polymeric column
Fig. 5-1 Column durability at pH12 comparison between CHP20/C04 and an ODS column
�T�h�e� �p�o�l�y�m�e�r�i�c� �R�P� �c�o�l�u�m�n�s� �a�r�e� �c�h�e�m�i�c�a�l�l�y� �s�t�a�b�l�e�.� �S�p�e�c�i�f�i�c�a�l�l�y�,� �t�h�e� �c�o�l�u�m�n�s� �h�a�v�e� �r�e�s�i�s�t�a�n�c�e� �t�o� �a�n� �a�l�k�a�l�i�n�e� �e�l�u�e�n�t�.� �T�h�e� �f�o�l�l�o�w�i�n�g� �g�r�a�p�h�s� �d�e�m�o�n�s�t�r�a�t�e� �s�t�a�b�i�l�i�t�y� �o�f� �t�h�e� �p�o�l�y�m�e�r�i�c� �c�o�l�u�m�n�s�.� �A�f�t�e�r� �f�e�e�d�i�n�g� �a� �s�o�l�u�t�i�o�n� �o�f� �p�H� �1�2� �i�n�t�o� �t�h�e� �M�C�I� �G�E�L�™� �C�H�P�2�0�/�C�0�4�,� �t�h�e�r�e� �i�s� �n�o� �c�h�a�n�g�e� �o�f� �c�o�l�u�m�n� �p�e�r�f�o�r�m�a�n�c�e�.
100 150 200500
1,2
0.4
0.6
0.2
0.8
1
0
degr
adat
ion
ratio
fiowing time hr.
CHP20/C04
ODS column
100 150 200500
1,2
0.4
0.6
0.2
0.8
1
0
degr
adat
ion
ratio
fiowing time hr.
CHP20/C04
ODS column
:MCI GEL™ CHP20/C04 4.6mmI.D × 150mm:20mM Na2HPO4 pH12/CH3CN/=60/40:0.4:25℃:254nm:1000ppm Dimethyl phthalate 5µL
ConditionsColumn EluentFlow rateColumn temp.DetectionSample
COOH
NH2 H2N-CH2(CH2)2-CH3
Polystyrenic adsorbent Silica gel C4, C18 Polymethacrylic adsorbent
Polymethacrylic adsorbent Silica gel C4, C18
π-π interaction(double bond effect) Alkyl effect
Hydrogen bond effect Hydrogen bond effect
Hydrogen bond effect
HN
NH2
COOHNH
H2N
(CH2)n-CH3O
SiSi
H2N-CH2 HN NH
HN
OHO O
O OCOH
OSi
Si
H2NCOOH
NH2NH
HN
C
O O
O OC
C
Retentiveness in reverse phase mode
Hydrophobic interaction Interaction mechanism
Weak interactionStronge interaction
Hydrophobic interaction
Interaction mechanism
1
2
3
4
5
0 5 10 15 20 25 30 35 40 min.
1
2
3
0 5 10 15 20 25 30 35 40 min.
MeOOC COOMe
Phthalic acid dimethyl ester
EtOOC COOEt
Phthalic acid diethyl ester
PrOOC COOPr
Phthalic acid dipropyl ester
Application data of CHP series
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:50mM Na-phosphate pH2.0, 1.5% Hexanesulfonic acid / CH3CN=80/20:0.25:ambient:280nm:1. Epinephrine 2. Dopamine 3. 5-Hydroxy tryptophan 4. Serotonin 5. Tryptophan
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:H2O/CH3CN=50/50:0.75:60℃:254nm:1. Dimethyl phthalate 2. Diethyl phthalate 3. Dipropyl phthalate
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:H2O/CH3CN=10/90:0.4:25℃:275nm :1.Theophylline 2.Theobromine 3.Caffeine
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:0.1M NaH2PO4 pH2.0 CH3CN=88/12:0.3:25℃:275nm :1.Cinchonine 2.Quinine
Fig. 5-2 Separation of catecholamines Fig. 5-3 Separation of phthalic acid esters
HO
HOOH H
N
Epinephrine
HO
HO NH2
Dopamine
HO
NH
NH2
COOH
5-hydroxy tryptophan
HO
NH
NH2
Serotonin
12
3
0 5 10 15 20 25 min.
1
2
0 5 10 15 20 25 min.
Fig. 5-4 Purine alkaloids Fig. 5-5 Cinchona alkaloids
HN
N N
N
O
O
Theophylline
N
N N
HN
O
O
Theobromine
N
N N
N
O
O
Caffeine
N
HOHH
H
H
N
Cinchonine
N
HOHH
H
H
N
O
Quinine
2
3
4
5
6
8
9
10
1
3
4
5
6
8
9
10
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2
4
5
6
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9
10
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2
3
5
6
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9
10
1
2
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10
1
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5
8 8
9
10
1
2
3
4
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6
9 9
10
1
2
3
4
5
6
10 10
1
2
3
4
5
6
8
1
2
3
4
5
6
8
7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
2
4
5
6
7
8
9
1
2
3
5
6
7
8
9
1
2
3
4
6
7
8
9
1
2
3
4
5
7
8
9
1
2
3
4
5
6
8
9
1
2
3
4
5
6
7
9 9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
35 36
Application data of CHP series
Comparison with an ODS column
Fig. 5-6 Uric acid and related compounds
Fig. 5-7 Bile acidsCHP20/C04
ODS column
1
2
3
1
3
2
13
2
4 5
0 5 10 15 20 min.
0 5 10 15 20 min.
0 5 10 15 20 min.
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:10mM TBA/CH3CN=75/25:0.4:25℃:284nm :1.Hypoxanthine 2.Xanthine 3.Theophylline 4.Uric acid 5.Orotic acid
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:50mM NaH2PO4 pH2.0 /CH3CN=40/60:0.3:25℃:210nm:1.Cholic acid 2.Ursodeoxycholic acid 3.Deoxycholic acid
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:ODS column (5μm) 4.6mm I.D.×150mm:50mM NaH2PO4 pH6.0 /CH3CN=40/60:0.3:25℃:210nm:1.Cholic acid 2.Ursodeoxycholic acid 3.Deoxycholic acid
N
N NH
HN
O
Hypoxanthine
HN
N N
N
O
O
Theophylline
HN
NH
N
HN
O
O
Xanthine
NH
HN
COOH
O
Orotic acid
O
HN
NH
N
HN
O
O
HO
Uric acid
H
OHH
COOH
HO
Ursodesoxycholic acid
H
OHH
COOH
HO
cholic acid
OH
H
H
COOH
HO
deoxycholic acid
OH
Application data of CHP series
Fig. 5-9 Adrenal cortex hormones
Fig. 5-8 Glycyrrhizae radix
min.
min.
Glycyrrhizic acid
0 30 35 4020 2515105
2
3
1
0 3020 2515105
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:2.06% acetic acid/CH3CN =63/37:0.5:45℃:254nm:Extract of glycyrrhizae radix
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:H2O/CH3CN:60/40:0.5:45℃:280nm:1.Hydrocortisone 2.Corticosterone 3.11-Deoxycortisol
Glycyrrhizic acid
HO
HO
H
H
O
H
OH
OOH
HOH
H
HO H
O
H
OHO
HOH
HOH H
HO HHO
O
OHO
HOOH
O
H
H H
O
HOOH
O
H
H H
OHO
OH
O
H
H H
hydrocortisone corticosterone 11-deoxycortisol
2
3
4
5
6
8
9
10
1
3
4
5
6
8
9
10
1
2
4
5
6
8
9
10
1
2
3
5
6
8
9
10
1
2
3
4
6
8
9
10
1
2
3
4
5
8 8
9
10
1
2
3
4
5
6
9 9
10
1
2
3
4
5
6
10 10
1
2
3
4
5
6
8
1
2
3
4
5
6
8
7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
2
4
5
6
7
8
9
1
2
3
5
6
7
8
9
1
2
3
4
6
7
8
9
1
2
3
4
5
7
8
9
1
2
3
4
5
6
8
9
1
2
3
4
5
6
7
9 9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
37 38
Comparison with an ODS column
Fig. 5-10 Ingredients of medicine
Fig. 5-11 Sulfa drugs
1
2
3
4
0 3020 2515105 min.
1
2
34
0 3020 2515105 min.
Application data of CHP series
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm
:20mM phosphate pH6.8/CH3CN =82/18:0.5:45℃:254nm:1.Succinylsulfathiazole 2.Sulfanilamide 3.Sulfathiazole 4.Sulfamerazine
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm:50mM phosphoric acid(pH2.0)/CH3OH =60/40:0.5:45℃:254nm:1.4-Dimethylaminoantipyrine 2.Antipyrine 3.Caffeine 4.Aspirin 5.Phenacetin
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:ODS column 4.6mm I.D.×150mm
:20mM phosphate pH6.8/CH3CN =90/10:0.5:45℃:254nm:1.Succinylsulfathiazole 2.Sulfanilamide 3.Sulfathiazole 4.Sulfamerazine
min.
1
2
3
4
5
0 3020 2515105
NN
O
Antipyrine
NN
O
DimethylaminoantipyrineAminopyrine
N
COOH
OAc
Aspirin
NHAc
O
Phenacetin
N
N N
N
O
O
Caffeine
S
HN
OO
N
N
NH2
Sulfamerazine
S
HN
OO
N
S
HN
OOH
O
Succinylsulphathiazole
S
HN
OO
N
S
NH2
sulphathiazole
SH2N
OO
NH2
Sulfanilamide
Application data of CHP series
0 108642 min.min.
2
1
3
4
Fig. 5-15 Water-soluble vitaminsFig. 5-14 Procainamide, Procaine
1
2
0 252015105
O
ON
H2N
Procaine
NH
ON
H2N
Procainamide
NH2
O
N
Vitamin B3
C-NCo3+
N
N-
N
N
N
NO
H2N
O
H2N
O
NH2O
OH
H2N
O
OHN
NH2
O
NH2
O
OP
HO
O-OO
Vitamin B12
NH
NHOOH
N
HO
OS
ONH
O
NH2HO
ONH
O
NH2HO
OH
O
NHN
N
O
NH
ONH
HN
O
HO
O
NH
O
NHOH
ONH
O
NH2
NH
HO
O
NH
H2N
H
0 108642 min.
2
1
3 4
0 40302010 min.
2
1
3
HN
HN O
OHN
HO O
ONH
O
NH2HO
N
OHHO
HO
Vitamin B6
OHOHO
OHHO
OH
Vitamin C
Gly-Tyr
Met Enkephalin
Leu Enkephalin
Angiotensin ll
Fig. 5-12 Peptides Fig. 5-13 Proteins
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm
:8mM Na2HPO4 pH7.0/CH3CN =85/15:0.5:25℃:254nm:1.Vitamin C 2.Vitamin B6 3.Vitamin B3 4.Vitamin B12
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm
:20mM phosphate pH7.2/CH3CN =65/35:0.5:45℃:254nm:1.Procainamide 2.Procaine
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm
:0.1%TFA/CH3CN =70/30:0.5:25℃:220nm :1.Gly-Tyr 2.Met Enkephalin 3.Leu Enkephalin 4.Angiotensin ll
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
:MCI GEL™ CMG20/C04 4.6mm I.D.×150mm:A;0.05%TFA/CH3CN =80/20 B;0.05%TFA/CH3CN =20/80 A→B 30min.linear:0.5:25℃:280nm:1.Ribonuclease A 2.Cytochrome c 3.α-Chymotrypsinogen A
2
3
4
5
6
8
9
10
1
3
4
5
6
8
9
10
1
2
4
5
6
8
9
10
1
2
3
5
6
8
9
10
1
2
3
4
6
8
9
10
1
2
3
4
5
8 8
9
10
1
2
3
4
5
6
9 9
10
1
2
3
4
5
6
10 10
1
2
3
4
5
6
8
1
2
3
4
5
6
8
7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
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9
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10 10 10 10 10 10 10 10
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3
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5
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9
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11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
39 40
Application data of CHP series
Pravastatin sodiumSimvastatin
MevastatinMevastatin
Impurity Impurity
SimvastatinPravastatin sodium
Nystatin
Amphotericin BAmphotericin B
Nystatin
Filipin
Filipin
OHO OH
O
HO
ONa
OH
Pravastatin sodium
O
O O
OHO
H
(+)-Simvastatin, Simvastatin
O
O
OHO
O
H
(+)-Compactin; Mevastatin
Fig. 5-16 Pravastatin sodium
CHP20/C10 10μm ODS 10μm
min.0 5 10 15 20 25 min.0 5 10 15 20 25
min.0 5 10 15 min.0 5 10 15
CHP20/C10 10μm ODS 10μm
Fig. 5-17 Polyene antibiotics
UV
238
nm (A
U)
UV
238
nm (A
U)
:MCI GEL™ CHP20/C10 (10μm 250 ×4.6mm I.D.) and ODS (10μm 250 ×4.6mm I.D.) : A :0.1% Formic acid; B :0.1% Formic acid in AcCN; A/B=60/40;:1.00:25℃:UV305nm for Nystatin, VIS405nm for Amphotericin B and UV340nm for Filipin; :Pravastatin sodium, Mevastatin and Simvastatin, 1mg/ml each;. :10μl
ConditionsColumn
Eluent
Flow rateColumn temp.Detection
Sample
Injection
:MCI GEL™ CHP20/C10 (10μm 250 ×4.6mm I.D.) and ODS (10μm 250 ×4.6mm I.D.) : A :0.1% Formic acid; B :0.1% Formic acid in AcCN; : 45%B-95%B over 29min.:1.00:25℃:UV238nm :Pravastatin sodium, Mevastatin and Simvastatin, 1mg/ml each;.:5μl
ConditionsColumn
Eluent
GradientFlow rateColumn temp.DetectionSampleInjection
OHOH
O
O
OH
OH
OH OH OH OH
OH
H
Filipin
O
O
O
OH OH OH OH
OH
OHOH
OH
O OH
O
O
OHH2N
HO
Nystatin
O
O
OHH2N
HO
HO
HO
OHO O OH OHOH OH OH
OH
O O
Amphotericin B
min.0
4
3
2
1
10 20 30
5
Application data of CHP series
CHP20/C10 10μm ODS 10μm
Fig. 5-19 Proteins
Ribonuclease A Conalbumin Ribonuclease A Conalbumin
ConditionsColumn Eluent
Flow rateColumn temp.DetectionSampleInjection
:150 ×4.6mm I.D. : A :0.1% TFA; B :0.1% TFA in AcCN:1.00:20%B-60%B over 20min;:UV280nm; :Ribonuclease A and Conalbumin 2mg/ml;. :10μl
Fig. 5-18 Proteins
:MCI GEL™ CMG20/C10 4.6mm I.D.×250mm:A 0.05% TFA/CH3CN=80/20 B 0.05% TFA/CH3CN=30/70 A → B 45min linear gradient:0.5:25℃:280nm:1. Ribonuclease A 2. Cytochrome C 3. Transferrin 4. α-Chymotrypshinogen A 5. β-Lactoglobulin
ConditionsColumn
Eluent
Flow rateColumn temp.DetectionSample
min. min.0 105 15 20 25 0 105 15 20 25
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7 7 7 7 7 7 7 7 7
9
1
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9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
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9
1
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5
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7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
41 42
ConditionsColumn
Eluent
GradientFlow rateColumn temp.DetectionSampleInjection
:
: : :::::
MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmA) 0.1%TFA, H2OB) 0.1%TFA, AcCN10%B→60%B over 25min.1.040℃230nmGhrelin rat and Ghrelin human ,0.1mmol/l each10μl
Fig. 5-21 Ghrelin
Application data of CHP series
CMG20/C10 10μm ODS 10μm
CHP20/C10 10μm
ODS 10μmCMG20/C10 10μm
Insulin glargine
UV2
30nm
(AU
)U
V230
nm(A
U)
UV2
30nm
(AU
)
UV2
30nm
(AU
)
UV2
30nm
(AU
)
Insulin human recombinant
Insulin glargine Insulin human recombinantInsulin glargine Insulin human recombinant
Ghrelin rat Ghrelin humanGhrelin rat
Ghrelin human
0 5 10 15 20min.
0 5 10 15 20min.
0 2 4 6 8 10 1412 1816 20min.
0 2 4 6 8 10 1412 1816 20min.
0 5 10 15 20min.
ConditionsColumn
Eluent
GradientFlow rateColumn temp.DetectionSampleInjection
:MCI GEL™ CHP20/C10 MCI GEL™ CMG20/C10 ODS 10μm 4.6mm I.D.×150mm:A) 0.1%TFA, H2O B) 0.1%TFA, CH3OH:20%B→60%B over 20min.:1.0:40℃:280nm:Insulin Glargine and human recombinant , 1mg/ml each:10μl
Fig. 5-20 Insulin
Application data of CHP series
CMG20/C10 10μm ODS 10μm
TargetU
V210
nm(A
U)
Impurity
Target
Impurity
0 5 10 15 20 25 30 35 40min.
UV2
10nm
(AU
)
0 5 10 15 20 25 30 35 40min.
ConditionsColumn
EluentFlow rateColumn temp.DetectionSampleInjection
:
::: :::
MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mm0.1%TFA,/CH3CN=68/321.0ml/min40℃210nmSifuvirtide crude(purity 35.5%) 2.1mg/ml0.4ml
Fig. 5-23 Sifuvirtide
Fig. 5-22 Leuprorelin
ConditionsColumn
Eluent
GradientFlow rateColumn temp.DetectionSampleInjection
MCI GEL™ CHP20/C10MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmA) 0.1%TFA, H2OB) 0.1%TFA, AcCN20%B→60%B over 20min.1.040℃280nmLeuprorelin,LHRH human, LHRH salmon and Buserelin ,1mg/ml each10μl
:
: ::: :::
0 2 4 6 8 10 12 14 min. 0 2 4 6 8 10 12 14 min.
Leuprorelin LeuprorelinBuserelin
Buserelin
Leuprorelin Buserelin
LH-RH salmon LH-RH salmon
LH-RH human
LH-RH human
LH-RH salmon
LH-RH human
CMG20/C10 10μm
CHP20/C10 10μm
UV2
30nm
(AU
)
ODS 10μm
UV2
30nm
(AU
)
0 2 4 6 8 10 12 14 min.
UV2
30nm
(AU
)
2
3
4
5
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7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
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9
1
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8
1
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3
4
5
6
7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
43 44
Application data of CHP series
Fig. 5-25 Nucleotide
Fig. 5-24 ssRNA Ladder Marker
ConditionsColumn
Eluent
Gradient
Flow rateColumn temp.DetectionSampleInjection
:MCI GEL™ CMG20/C10 ODS 10μm 4.6mm I.D.×150mm:A)100mM TEAA, H2O B)100mM TEAA, CH3CN:CHP10/C10 ODS 10μm:1.0:40℃:260nm:14-30 ssRNA Ladder Marker [max.0.04mg/ml]:5μl
10%B→40%B over 30min8%B→40%B over 30min
0-4.0min 0%B 4.0-5.0min 0→30%B 5.0min-6.0min 30%B 6.0min-7.0min 30→50%B7.0min-10.0min 50→65%B 10.0min-11.0min 65%B 11.0min- 0%B
1.Ura,2.Xan,3.Thy,4.Hyp,5.Gua,6.Cyt,7.Ade, 8.Urd,9.Xao,10.dT,11.Ino,12.Guo,13.Cyd,14.Ado
B)20 mM Na2HPO4 / 100 mM NaClO4 / 30% ACN
ConditionsColumn
Eluent
Gradient
Flow rateColumn temp.DetectionSampleInjection
:MCI GEL™ CHK40/C04 4.6mm I.D.×150mm:A)19 mM H3PO4 / 1 mM NaH2PO4 / 5.0% ACN : :0.8:50℃:UV260nm::20μl
tR / mim
inte
nsity
/ -
Ura
Urd
Xan
Xao
Ino Th
y
dT
Hyp
Guo
Gua
Ade
Cyd
Cyt
Ado
0 2 4 6 8 10 12 14
0 5 10 15min.
ODS 10μm 40℃
CHP20/C10 40℃
CHP20/C10 60℃
UV
260n
m(A
U)
18 base
14 base22 base
26 base30 base
(Data provided by Professor Yokoyama of Yokohama National University)
Application data of CHP series
LinaloolUV2
10nm
(AU
)
0 2 864 10分
CMG20/C10 10μm
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
Injection
:
:::::
:
Fig. 5-26 Linalool
0 5 10 15 20min.
CMG20/C10 10μm
UV2
10nm
(AU
) 12
3
4 5
0 5 10 15 20min.
ODS 10μm
UV2
10nm
(AU
)
1
2
3
4
5
ConditionsColumn
EluentFlow rateColumn temp.DetectionSampleInjection
:MCI GEL™ CMG20/C10 4.6mm I.D.×150mm:Hexan/Ethanol=99.5/0.5:1.0:40℃:210nm:Coriander:10μl
MCI GEL™ CMG20/C10ODS 10μm4.6mm I.D.×150mmHexan/Ethanol=99.5/0.51.040℃210nm1:Linalyl Acrtate 1mg/ml2:Linalool 1mg/ml3:β-Citronellool 1mg/ml4:Nerol 0.5mg/ml5:Geraniol 0.5mg/ml10μl
Fig. 5-27 Coriander
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7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
2
4
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9
1
2
3
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9
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9
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2
3
4
5
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8
9
1
2
3
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9
1
2
3
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5
6
7
9 9
1
2
3
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5
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7
8
1
2
3
4
5
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8
9
1
2
3
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5
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7
8
1
2
3
4
5
6
7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
45 46
Application data of CHP series
(TritonX-100)
min. min.
Fig. 5-32 ODS-1HU (ODS)Fig. 5-31 C18-alkylated aliphatics CHPOD/C04
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ CHPOD/C04 4.6mm I.D.×150mm:50vol%CH3CN:0.50:40℃:254nm:Triton X-100 (Polyoxyethylene octyl phenyl ether) 1% each 10μL
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ ODS-1HU 4.6mm I.D.×250mm:50vol%CH3CN:1.00:40℃:254nm:Triton X-100 (Polyoxyethylene octyl phenyl ether) 1% each 10μL
n=9.6 n=9.6
Fig. 5-33 Application data of Nucleic base/Nucleoside and Beer
0-0.7min 0%B 0.7-3.0min 0→40%B 3.0-3.2min 40%B3.2-3.5min 40→80%B 3.5-8.0min 80%Β 8.0min- 0%B
ConditionsColumn
Eluent
Gradient
Flow rateColumn tempDetectionInjection
:MCI GEL™ CHK45/C05 4.6mm I.D.×150mm:A)8 mM H3PO4
B)10 mM H3PO4 /30% ACN
:1.3:45℃:UV(260nm):20μl
1211109876543210
1211109876543210
1211
Draft Beer
Carbohydrate-free "happoshu-type" beer
A new category of beer that contains 85% less purine
109876543210
Analysis of various category beer
Urd
Ura
dT
Cyd
Urd
Urd
Ura
dT Cyd
Ino
Xan
Guo
Gua
dA Ade
Ino
Ura Ino
Xan
dT Xan
Guo
Guo
Gua
Gua
dA
Ade
0
Inte
nsity
/-
Ino
Ura
Xan
Hyp
Xao
Urd
AMP
UM
P
Cvd
Guo
dT
GD
P
Cvt
dCG
ua
dA
Ade
Ado
tR / min2 4 6 8 10 12
(Data provided by Professor Yokoyama of Yokohama National University)
Application data of CHP series
(Polyphenon 60)Fig. 5-30 Styrene Divinylbenzene CHP20/C04Fig. 5-29 Modified Styrene Divinylbenzene
CHP07/C04ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ CHP20/C04 4.6mm I.D.×150mm:CH3OH/10mM-Acetic acid=60/40:0.46:60℃:280nm:Polyphenon 60(10mg/ ) each 10μL
ConditionsColumn
EluentFlow rateColumn temp.DetectionSample
:MCI GEL™ CHP07/C04 4.6mm I.D.×150mm:CH3OH/10mM-Acetic acid=60/40:0.46:60℃:280nm:Polyphenon 60(10mg/ ) each 10μL
(Data provided by Professor Yokoyama of Yokohama National University)
Fig. 5-28 Application data of CHK40/C04:Sepatation of Sartans
N
O
HO
O
NH
N
NN
Valsartan
N
ON
HO O
NHNNN
Candesartan
O
N
N
HN NN N
Irbesartan
NN
HO
Cl NHNNN
Losartan
ConditionsColumn
Eluent
Gradient
Flow rateColumn temp.DetectionSample
Injection
:MCI GEL™ CHK40/C04 4.6mm I.D.×150mm: :
:1.0:50℃:UV:Candesartan(CAN),Losartan(LOS), Valsartan(VAL), Irbesartan(IRB) :20μl
A)10 mM Na H↓ 2 PO↓ 4 +0.2 mM Na↓ 2 HPO↓ 4 (25%ACN)B)10 mM Na H↓ 2 PO↓ 4 +1.0 mM Na↓ 2 HPO↓ 4 (40%ACN)0.5min 0%B 0.5-2.0min 50%B2.0min- 90%Β
min.min.
min.642
CAN
LOS
VAL
IRB
0
2
3
4
5
6
8
9
10
1
3
4
5
6
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9
10
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10
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1
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1
2
3
4
5
6
8
7 7 7 7 7 7 7 7 7
9
1
2
3
4
5
6
8
7
9
10
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n list
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
SPE sorbent seriesIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Chiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
MCI GEL™ colum
n listChiral separationcolum
ns
SPE sorbent series
SPE sorbent series
SPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n list
SPE sorbent series
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsCom
pounds indexMCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
Compounds index
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
ns
Analytical and preparative chromatography columns and materials for pharm
aceutical applicationsMCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ material list
MCI GEL™ material list
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
SPE sorbent seriesChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Compounds index
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ colum
n listChiral separationcolum
nsAnalytical and preparative chromatography columns and materials for pharm
aceutical applications
Chiral separationcolum
nsSPE sorbent series
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
MCI GEL™ colum
n listBioseparation colum
ns and m
aterialsIon chrom
atography colum
ns and materials
Ion exchange columns
and materials
Column selection
guide
MCI GEL™ material list
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
Compounds index
MCI GEL™ material list
Bioseparation columns
and materials
Ion chromatography
columns and m
aterialsIon exchange colum
ns and m
aterialsColum
n selectionguide
Analytical and preparative chromatography columns and materials for pharm
aceutical applications
2
3
4
5
6
7
8
9
1
3
4
5
6
7
8
9
10 10
1
2
4
5
6
7
8
9
1
2
3
5
6
7
8
9
1
2
3
4
6
7
8
9
1
2
3
4
5
7
8
9
1
2
3
4
5
6
8
9
1
2
3
4
5
6
7
9 9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
1
2
3
4
5
6
7
8
1
2
3
4
5
6
7
8
9
10 10 10 10 10 10 10 10
1
2
3
4
5
6
9
10
11
カラム選択の手引き
イオン交換クロマトグラフィー用
充填カラム及び充填剤
イオンクロマトグラフィー用
充填カラム及び充填剤
生体高分子分離用
充填カラム及び充填剤
医薬精密精製用充填カラム
及び充填剤
アミノ酸DL分離用
充填カラム
MCI
GEL
カラム一覧
MCI
GEL
充填剤一覧
分離例化合物リスト
7
固相吸着剤
47 48