1

SDM COLLEGE OF MEDICAL SCIENCES AND HOSPITAL

Manjushree Nagar, Sattur, Dharwad – 580009

Phone: (0836) 247777

HANDS ON DNA PCR TRAINING WORKSHOP

FOR MICROBIOLOGY, BIOTECHNOLOGY, BIOCHEMISTRY STUDENTS

19TH TO 21ST February 2015

Organised by

SDM College of Medical Sciences & Hospital, Dharwad

Venue

Department of Microbiology

Third Floor, College Building,

SDM College of Medical Sciences and Hospital, Dharwad

2

SDM COLLEGE OF MEDICAL SCIENCES AND HOSPITAL, DHARWAD

Hands on training workshop on DNA-PCR

Programme Schedule:

DAY 1: (19th February 2015)

1 8.30 am Registration and Breakfast

2 9.00 am INAUGURATION CEREMONY

3 10.00 am Introduction to PCR - History, What is PCR?, Types of PCR and Applications of PCR –Dr. Reeta Mani

4 11.00 am Organisation of PCR Laboratory, Instruments and Guest lecture equipment, Chemicals and reagents – Dr. Ajantha G.S.

5 12.00 pm Tea break

6 12.10 pm Extraction protocol and beginning of DNA extraction – Dr. Anuradha S.K.

7 LUNCH BREAK

8 02.00 pm Hands on experience – DNA extraction

9 4.00 pm TEA

3

DAY 2: (20th February 2015)

10 9.00 am Real time PCR – Dr. Reeta Mani

11 10.00 am Hands on experience – DNA extraction

12 11.00 am Tea break

13 11.10 am Washing, sterilization and waste disposal – Dr. Praveen C. Shetty

14 12.00 am Demonstration - Spin column based extraction – Mrs. Radhika Madalgi

15 LUNCH BREAK

16 02.00 pm Hands on experience: Preparation of PCR mix and amplification – Dr. Suma

17 3.00 pm Real time PCR - Demonstration of machine, discussion and reviewing old runs and Gradient PCR – Dr. Deepa Patil

18 4.00 pm GROUP PHOTO AND TEA

4

DAY 3: (21st February 2015)

19 9.00 am Hands on experience: Preparation of PCR mix and amplification - Dr. Suma

20 9.30 am Trouble shooting, dos and don’ts of PCR including handling of samples – Dr. Shubhada C.

21 10.00 am Internal and external quality control, storage of samples, storage of DNA and record keeping - Dr. Manjunath A.H.

22 11.00 am Tea break

23 11.10 am Hands on experience: Preparation of gel, DNA electrophoresis –Dr. Pavithra Jain

24 12.00 am Hands on experience: Preparation of gel, DNA electrophoresis –Dr. Pavithra Jain

25 LUNCH BREAK

26 02.00 pm Hands on experience: Reading the runs and gel documentation - Dr. Ajantha G.S.

27 3.00 pm Hands on experience: Reading the runs and gel documentation - Dr. Ajantha G.S.

28 4.00 pm VALEDICTORY FUNCTION

EXPERTS FOR LABORATORY WORK:

1. Mr. Umesh Biradar

2. Mr. Avinash Kedari

SDM COLLEGE OF MEDICAL SCIENCES AND HOSPITAL

Manjushree Nagar, Sattur, Dharwad – 580009

Work shop date 4/10/2015

Live Laparoscopy workshop conducted by Dept of OB & GYN SDM and Hubli Dharwad OB &

GYN Society at SDM Medical College Faculaty.

19th and 20th December 2015

RCOG Basic Practical skill course conducted by Dept of OBGYN SDM CMSH Dharwad in

Association with Bharatbhoomi Medical Academy, Dharwad.

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