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Computer AssistedSemen Analysis
Touch smart interface
Soft/Hard copy Reports
Wi-Fi Tablet Support
Bar-Code Support
Integrated Sperm Chemistry Analyzer
Computer Assisted Semen Analysis System utilizes modern computer and advanced image processing techniques for clinical test of sperm motility, quality, morphology, vitality, DNA fragmentation, HOS and nuclear protein. Based on the sperm test standards of the WHO 5th criteria (2010), the system can analyze the characteristic of sperm comprehensively through image processing of sperms. The system analyzes these values and generates accurate parameters to reflect sperms’ quality. The whole procedure is fast and provides several important information that are vital to present scientific basis for the male reproductive ability.
Spermolyzer
NATURALLY SHINE
1- Dynamic standard method :• The method is designed to estimate the spermatozoa concentration and their motility in a native specimen. • Determining spermatozoa concentration in ( million/ml)• Measuring spermatozoa movement parameters and classify this movement to classes in accordance of WHO
recommendations .• Measuring spermatozoa dynamic parameters ( VCL/VSL/VAP/MAD/ALH/BCF/LIN/WOB/STR) 2- Morphology standard method:• The method is designed to determine the morphological parameters of Spermatozoa.• The software calculate the ( TZI/SDI/MAI) according to the latest criteria Of the world health organization.
3- Vitality test:This test is important to determine if the non-motile spermatozoa are alive or dead. It should be assessed if a low percentage of sperm are progressively motile, e.g., 30 - 40%.The two most common methods of sperm vitality assessment: • Eosin-nigrosin staining. • The hypo-osmotic swelling test ( HOS).
4- Sperm DNA Fragmentation Test:This test provides a reliable analysis of sperm DNA integrity that may help to identify men who are at risk of failing to initiate a healthy ongoing pregnancy. Information about sperm DNA integrity may help in the clinical diagnosis, management and treatment of male infertility and may be of prognostic value in assessing outcome of assisted conception treatment.
5- HOS: The hypo-osmotic swelling test is based on the semi-permeability of the intact cell membrane, which causes spermatozoa to “swell” under hypo-osmotic conditionsAll the curled tail sperm are labeled as ‘Live Sperm’. The damaged / brokensperm membrane, allows fluids to pass across the membrane without anyaccumulation & hence there is No Cytoplasmic Swelling & the tail remains straight(No Curling). All these sperm are labeled as the Dead sperm.
6- Nuclear Protein Assay:The mature chromatin contains around 85% Protamine & 15% Histone, whereas immature chromatin contains more than 15% Histone.Increased levels of persistent Histone in turn indicate a break in the development sequence of Histone – Transition Protein – Protamines, which significantly affect the DNA chain folding & vulnerability for increased DNAFragmentation & this leads to decline in sperm fertilizing potential. Hence by evaluating Nucleoprotein assessment the diagnosis & prognosis of Male Infertility can be determined.
Creative Constant-Temperature Operation DeskThe operation desk makes provides a constant temperature of 37 during sperm testing during the whole test procedure. This in turn eliminates the influence of excessive low outside temperature on the tested sperm such as sperm velocity, sperm vitality etc. Unique Virtual Grid FunctionThis function has realized the direct comparison of test results between human being and machine in the system. If you wish to use manual counting there is no need to buy an expensive calibrated counting chamber, it is all in your screen with the aid of virtual grid function
Database:• The in-built database enable the user to store, backup and restore any kind Of obtained information .• The in-built database enable the user to generate historical report for any Patient.• The in-built database is capable of helping researcher by generating many Statistics reports.
Other Features of Spermolyzer software:• Spermolyzer software is able to automatically add appropriate comments according to the patient results.• Spermolyzer software has the ability to generate accustom reports like Urine, Stool, CBC and Bone marrow
with any captured image using the system digital camera and microscope.•Spermolyzer software allows the user to record and print the manual test result of semen.
Standard Methods:
• Spermolyzer includes two standard methods ( Dynamic and Morphology)
• The analysis of the parameters is performed according to the recommendations given by The
world health organization (WHO) for the latest 5th edition for motility patterns & Morphometric
assessment of human semen.
System Requirements
Computer
Operating system
Processor
HDD
Ram
Display Adapter
Slide
Camera
Heater
Microscope
Desktop, Laptop or All in one PC
Windows 7 or 8.1 (32 or 64 bits)
Intel core i3 or Higher
1 TB or Higher
4 GB DDR3 or Higher
ntel Integrated graphics or ATi or nvidia 1GB
Special Slide for count and motility
USB 3.0 industrial CMOS digital camera 30 frame /sec640 x 480 minimum resolution
Special temperature controller with attached heateron the microscope stage
Olympus, Micros, Nikon, Zeiss, Leica C-mount 1x, Trinocular tube 1x,Objective : 10x, 20x, 40x, 100x oil plan achromatic lens.
Sperm Vitality
Kit
Andrology Solution
Kit
Sperm Function Test Kit
Spermolyzer (Smart CASA
system)
Viscosity Reagnent
Kit
Sperm Fructose Kit
Sperm pH strips Sperm
HOS reagent
Kit
Sperm Morphology
Kit
Sperm Vitality
TestSperm
Dynamic Test
Sperm Morphology
Test
HOSTest
DNA fragmentation
Test
Zink determination
kits
Nuclear protein
assessment kits
Antisperm antibody
(IgG & IgA) Reagent
Kits
–Glycosidase
kits
Leukocytes Confirmation
kits
DNAfragmentation
reagent Kit
Mediumfor spermSwim up
technique
Semen processing
solutions
Mediumfor sperm
Swim down technique
IUI Catheter.
Non- Spermicidal
semen collection
pouch
Andrology lab will be most Easy and Practical….With specially developed instruments & accessories for all sperm function tests considering their specific
functional and ergonomical needs.The instruments are developed considering in vivo temperature environment of the natural processes,
Where ever needed which brings systematic approach and give quality test results.
Spermolyzer (Smart CASA system):Spermolyzer is a computer assisted semen analysis System which utilizes modern computer and advanced image processing techniques for clinical test of sperm based on the standards of the WHO 5th strict criteria.
Spermolyzer Software support:* Sperm Dynamic Test. * Sperm Morphology Test.
* DNA fragmentation Test. * HOS Test.
* Sperm Vitality Test.
Warmer for Semen specimen:Semen specimen warmer is a temperature control system which is ergonomically designed to give ease of work and specimen handling. The system has heater & sensor programmed to attain & maintain 37°C. It has very high accuracy with ± 0.20C.
Warmer for DNA fragmentation Test.: DNA fragmentation test warmer is a dual temperature control system, designed to expose the agarose tubes at 70°C or at 37°C in separate blocks of instrument. The two blocks for two different temperatures can be used simultaneously.You can thereby cut off the hassle included in conventional method and do the work in ease in just one instrument.Also, Its ergonomics allows user to handle each tube without disturbing others.
Sperm Centrifuge 37°C: Sperm centrifuge machine designed to work at 37°C, that is same as the in-vivo temperature, to maintain the natural functionality of sperm.
Technical specifications: •Maximum speed: 4000 RPM (with rotor 4x 15ml.)
•Maximum RCF: 2,719 X g (with rotor 4x 15ml.)
•Maximum sample volume: 60 ml.
•Speed setting & display: Digital.
•Speed accuracy :± 100RPM.
MIRALAB ANDROLOGY LINE
Normal seminal profile
223Patient Name
Dr.\Galal Ibrahimص 10:31:13 25/02/2015201502251 Name
Lab ID
The system follows WHO (2010) strict criteria for motility patterns & morphometric assessment of human semen.
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
_
0
20 _
40 _
60
80
100
_
_
54.38
35.48
NP
10.14
IMPR
Case no. Date
Ref. by
Collection Abestination
Physical Proerties
pH Volume
Color Odor Viscosity Liquefaction time Liquefaction state
47.7Grey OpalescentNormalNormal20Complete
Test Result Test Concentration Total sperm count Progressive motility (PR)
Masturbation at lab4
Result Status Reference value
Total motility (PR+NP) Morphology index Vaitality
54.15216.654.3889.86
2463
PassedPassedPassedPassedPassedPassed
>= 15 (million/ml) >= 39 (million/ejaculate) >= 32 % >= 40 % >= 4 % >= 58 %
Agglutination & Aggregation Agglutination Agglutination grade Non-specific aggregation
Head to headIsolated ( < 10 spermatozoa per agglutinate, many free spermatozoa )Between active sperm and mucus thread
Other Cells White blood cells Red blood cells Spermatogenic cells Epithelial cells
Comment
2 - 44 - 611 - 2
The semen passed the W.H.O. strict criteria for count, motility and morphology.
Pinheads :
Pinheads sperms are not counted as spermatozoa orhead defects because they possess no chromatin orhead structure anterior to the basal plate.
million/ml1
>= 1.5 ml>= 7.2
Age
< 5 /H.P.F.< 5 /H.P.F./H.P.F./H.P.F.
2 - 5 days
15 - 60 min.
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.
Dynamic parameters (I) Classification Tested sperms
Progressive motility (PR)
Conc. (million/ml) Total number (million) Percentage %
Total motility (PR+NP)
Non progressive (NP) Immotile (IM)
54.15
29.4548.66
19.215.49
Dynamic parameters (II)
Patient Name 201502251
Motility Report
216.6
117.8194.64
76.8421.96
100
54.3889.86
35.4810.14
Progressive motility (PR)
Non-progressive motility (NP)
Immotile (IM)
Spermatozoa moving actively either linearly or in a large circle regardless of speed
All other patterns of motility with absence of progression, i.e. swimming in small circle, the flagellarforce hardly displacing the head, or when only a flagellar beat can be observed.
No movement
VCL (µm/s) 32.44 VSL (µm/s) 16.61 VAP (µm/s) 23.33
MAD (°) 57.57 ALH (µm) 2.99 BCF (Hz) 2.93 LIN (%) WOB (%) STR (%)
VCL VSL VAPCurvilinear velocity Straight line velocity Average path velocity
MAD ALH BCFMean angular degree Amplitude of lateral head displacement Beat-cross frequency
51.21 71.91 71.05
LIN WOB STRLinearity (VSL/VCL) Wobble (VAP/VCL) Straightness (VSL/VAP)
VSL
VCL
VAP
ALH
MAD
Head displacement
Sperm tracks and velocities Legend
DCL (µm) 26.49 DSL (µm) 13.53 DAP (µm) 19.04
DCL DSL DAPDistance curved line Distance straight line Distance average path
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.
Progressive velocity (VSL)
Patient Name 201502251
Charts Report
% %
µm/sµm/s 0-10 10-20 20-30 30-40 40-50 50-60 >60
51.18
21.9810.44 10.20
4.95 0.55 0.69
100
80
60
40
20
0
_
_
_
_
_
100
80
60
40
20
0
_
_
_
_
_
Track speed (VCL)
Path velocity (VAP)
100
80
60
40
20
0
_
_
_
_
_
% %
µm/s
100
80
60
40
20
0
_
_
_
_
_
Sperm distribution
Track image (I) Track image (II)
PR
54.38
35.48
NP
10.14
IM
0-10 10-20 20-30 30-40 40-50 50-60 >60
34.60
12.53 14.019.06 7.13 5.64
17.03
0-10 10-20 20-30 30-40 40-50 50-60 >60
39.16
15.25 15.79 14.94 10.603.57 0.69
PR (Progressive) NP (Non Progressive) IM (Immotile)
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Tail defectsNeck & midpiece defects
24 76
80
1612
0
00
0
00
0
00
4
0
432
52
0
0
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.Patient Name 201502251
Morphology Report
Class4.48
Head length
3.05Length/Width (%)
1.49
Head defects
Normal sperms (Morphology Index) Terato sperms
A. Head abnormalities
% %
Normal sperms (Morphology Index) Terato sperms
C. Principal piece (Tail) abnormalities%%
Normal sperms (Morphology Index) lblPyriformH
%%
Normal sperms (Morphology Index) Terato sperms % Vaculated Small acrosome
%%
Double head %
Big head Small head Tapered head Pyriform head Round head Amorphous
%
Normal sperms (Morphology Index) Terato sperms
%%
Normal sperms (Morphology Index) Terato sperms
%%
Normal sperms (Morphology Index) Terato sperms %
Short tail Bent tail Multi tail Break-down tail Coiled tail Irregualr tail
%
D. Excess residual cytoplasm (ERC)% E. R. C.
B. Neck & Midpiece abnormalities Normal sperms (Morphology Index) Terato sperms
%%
Normal sperms (Morphology Index) Terato sperms
%%
Thin neck Thick neck Sharply bent Asymmetrical insertion
MAI (Multiple Anomalies Index) :The mean number of anomalies per abnormal spermatozoon
1.68
TZI (Teratozoospermic Index) :Total number of defects divided by the number of abnormal sperms
1.32
SDI (Sperm Deformity Index) :Total number of defects divided by the number of sperms counted
1.28
Head width
Mean of normal spermsMean of terato sperms
Reference values
Head area Acrosome (%) Head perimeter
12.53 12.89 37.715.43 3.05 2 14 15.77 177
4 - 5.5 2.5 - 3.5 1.5 - 1.75 7.85 - 15.1 10.8 - 14.9 40 - 70
Tapered Pyriform Round
Amorphous Vaculated Small acrosome
Bent Asymm. insertion
Thick Thin
Short Bent
Coiled E.R.C.
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.Patient Name 201502251
Morphology Images
Normal sperm Thin neck Small acrosomeSmall headThin neck
Normal sperm Thick neck Small acrosomeBig head
Bent neck
Normal sperm Normal Sperm Small acrosome
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.Patient Name 201502251
Vitality Report
Vitality image (I) Vitality image (II)
Total sperms
Live sperms Dead sperms
954
55.5644.44
%#
100
_
0
20 _
40 _
60
80
100
_
_
Live
44.4455.56
Dead
Live Dead
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.Patient Name 201502251
Morphology Images
Normal sperm Thin neck Small acrosomeSmall headThin neck
Normal sperm Thick neck Small acrosomeBig head
Bent neck
Normal sperm Normal Sperm Small acrosome
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Non Fragmented Fragmented Degraded
Name
Sem
en S
cree
n (C
ompu
ter
Ass
iste
d Sem
en A
naly
sis)
Semen Screen (Computer Assisted Semen Analysis)
Case no.Patient Name 201502251
DNA Fragmentation Report
DNA fragmentation image (I) DNA fragmentation image (II)
Total sperms
Non fragmented Fragmented
451332
28.8971.11
%#
Without halo 9 20
%#
Small halo Medium halo 17
1437.7831.11
Big halo 1 2.22
Degraded 4 8.89
_
0
20 _
40 _
60
80
100
_
_
Non Fragmented
71.11
28.89
Fragmented
_
0
20 _
40 _
60
80
100
_
_
Big
2.22
37.78
Medium
8.89
Small
31.1120.00
Without DegradedHalo size
100
DNA Fragmentation Index
%15 > DFI15 < DFI < 3030 < DFI
Excellent fertility potential.Good fertility potential.Poor fertility potential.
DFI 28.89
Comment
The sample has a good fertility potential
HOSPITEXDiagnostics
HOSPITEXDiagnostics
Italy
Tel:+202 2504 4134 Fax:+202 2504 4135 Email:[email protected] www.mira-lab.com
Building No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Egypt & Middle East Head OfficeBuilding No.6105/6 Quds St, Mokattam , Cairo, EGYPT
Tel : +202 2504 4134 / +201150222203 Fax : +202 2504 4135
Alexandria Branch OfficeBuilding No4, Street No7 from El Naser St., Smouha, Alexandria, EGYPT
Tel : +2011 59 88 77 05
www.mi ra - l ab .comE-mail:[email protected]