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Anti Obesity Effects of P. guajavaonObese Induced Rats

Connie Jesse Anyi

2009710617

Supervisor:

PM Dr Hjh Farida Zuraina Hj Mohd Yusof


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Introduction

Obesity is a nutritional disorder resultedfrom excessive accumulation of adipose thatmay impair health if it is not control.

Causes of obesity is due to genetic andenvironmental factor.

Obesity is a strong factor that cause type 2diabetes which related to insulin resistance

of human. Most common risk that leads to diseases

such as cardiovascular problem, menstrualproblem and hypertension.


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Known as pink guava or jambu batu Tropical fruit from the family of Myrtaceae which

often found in a tropical countries (Burkill et al.,1997).

Often used as food, juice and folk medicine. Lately,there are many research has been done on its bark,roots, and fruits (Romeo et al1997).

This study will determine how P.guajavawill treat

obesity in obese induced rats.

Psidium guajava


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Literature Review

Studies done on the leaves indicated that it's aqueous extract haveanti hyperglycemic and hyperlipidemic effect on type II diabetespatient.

Ingestion of guava leaf tea will reduce the blood glucose level andtotal cholesterol (Deguchi & Miyazaki, 2010).

It is also a good source of fiber that contribute to the use of its fruitas anti diarrhea by relaxing the smooth muscle of intestine.

Its fruit puree has been confirmed to have anti hypertensiveproperties and has lipid lowering effect on high fat diet induced rats(Ayub et al., 2010 ) and anti inflammatory properties (Murugadan etal., 2010).


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Problem Statement

Malaysia is one Asian country that possess thehighest number of obese communities compared toother Asian country and obesity is the highest causethat leads to death in Asia. (world health organization)

Obesity affect both children and adults medicalhealth and mental.(Braet et al. 2005)

The use of drug in treating obesity has adverseeffects in some individual and the use of plantmedicine in treating obesity is still new.


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Significance of Study

There is less study focusing on the leaf of P.guajava. Many research done are focusing moreto the puree of this local fruit.

The importance of the leaf can be seen throughthe usage of P. guajavaleaf as traditionalmedicine mainly in South East Asia.

Gives the insight on how obesity in human canbe reduced by using safer and traditional way

rather than surgery or drugs.


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Objectives

To determine the effects of P.guajavaleafextract on the body weight of obese inducedrats.

To determine the effects of P.guajavaon theblood pressure of obese induced rats.

To determine the effects of P.guajavaonblood glucose level and lipid profile of obeseinduced rats.


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Preparation of animal sample

Induction of obesity in treatment groups

Treatmentgroups

Obese low (2%)Obese medium (6%)Obese high (10%)

Control group

Obese Non obese

Preparation of aqueous extract of P.guajava

Treatment of P.guajava leaves extract

Control group

Treatment

groups1. Changes in body weight2. Changes in blood pressure3. Blood glucose level4. Lipid profile

Data Analysis

METHODS AND MATERIAL


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Statistical analysis

1. Correlation and regression will be use to see therelationship between different concentration ofP.guajava leaf extract and:

body weight of obese induce rats blood pressure of obese induce rats

blood glucose level of obese induce rats

2. One way ANOVA

To compare means of different groups


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References1. Norazmir M.N., Ayub M.Y., & Mamot S (2009). Blood and Urine profiles of

spontaneous hypertensive rats supplemented with pink guava ( Psidium guajava)

puree. Sains Malaysiana 38(6): 929 934.

2. Anas, K., Jayasree, P.R., Vijayakumar, T., & Kumar, M.PR. (2007). In vitroantibacterial activity of Psidium guajava Linn.leaf extract on clinical isolates of

multidrug resistant Staphylococcus aureus. Indian journal of experimental

biology 46, 41 46.

3. Prashant, K.R., Jaishwal, D., Mehta, S., & Watal, G. (2009). Anti hyperglycaemic potential of Psidium guajava raw fruit peel. Indian J Med Res

129: 561

565.

4. Won, K.O., Chul H.L., Myung, S.L., Eun, Y.B, Cheon, B.S., Oh H., Bo Y.K., &Jong S.A.(2003). Antidiabetic effects of extracts from Psidium guajava.Journal

of Ethnopharmacology 96: 411 415.

5. Adeyemi, Stephen, O., Akanji, M.A., & Oguntoye, S.A. (2009). Ethalonic leafextract of Psidium guajava: Phytochemical and trypanocidal activity in rats

infected with Trypanosoma brucei brucei. Journal of Medicinal Plants

Research 3(5): 420 423

6. Muruganadan, S., Srinivasan, K., Tandan, S.K., Jawahar L., Suresh C., &Raviprakash, V. (2001). Anti inflamma tory and Analgesic Activities of some

Medicinal Plants. Journal of Medicinal and Aromatic Plant Sciences 4: 56 58.

7. Burkill, H.M. (1997): The useful plants of West Tropical Africa. Botanicgarden Kew 4: 52.


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