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Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation

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Page 1: Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation

Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

Page 2: Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation

Phase Contrast Images

Brightfield Phase Contrast

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Phase Contrast MicroscopyF. Zernike, 1930s

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Phase Contrast Microscopy

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Phase Contrast Images

Brightfield Phase Contrast

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Contrast Microscopy

Koehler Illumination

Dark Field

Phase Contrast

Differential Interference Contrast

Hoffman Modulation

Page 7: Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation

DIC (Nomarski) Images

Phase Contrast Nomarski Optics

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James Maxwell(1831-1879)

Electromagnetism

Electromagnetic Theory of Light

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Wave Nature of Light

Under some conditions, light behaves as an electromagnetic wave (Maxwell, 1855). In this model, electric and magnetic fields oscillate perpendicular to one another and to the direction of propagation of the light wave.

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Refractive Polarization

David Brewster(1781 - 1868)

Brewster Angle[53o - 56o]

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Optical Birefringence

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Differential Interference Contrast Microscopy

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DIC

PhaseContrast

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PhaseContrast

DIC

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Hoffman Modulation

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Hoffman Modulation Images

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