Control of Transcription

BASIC LIFE SCIENCESAlexander Hollaender, General EditorBiology DivisionOak Ridge National Laboratoryand The University of TennesseeKnoxville

1973: Volume 1 • GENE EXPRESSION AND ITS REGULATIONEdited by F. T. Kenney, B. A. Hamkalo. G. Favelukes.and J. T. August

Volume 2 • GENES. ENZYMES, AND POPULATIONSEdited by A. M. Srb

1974: Volume 3 • CONTROL OF TRANSCRIPTIONEdited by B. B. Biswas. R. K. Mandai, A. Stevens,and W. E. Cohn

In preparation:

• REPRODUCTIVE PHYSIOLOGY AND GENETICSEdited by F. Fuchs and E. M. Coutinho

A Continuation Order Plan is available for this series. A continuation orderwill bring delivery of each new volume immediately upon publication. Volumesare billed only upon actual shipment. For further information please contactthe publisher.

Control ofTranscription

Edited by

B. B. Biswas and R. K. MandaiBose InstituteCalcutta, India

and

A. Stevens and W E. CohnBiology Division

Oak Ridge National LaboratoryOak Ridge, Tennessee

PLENUM PRESS · NEW YORK- LONDON

Library of Congress Cataloging in Publication Data

Calcutta Symposium on Control of Transcription, 1973.Control of transcription; [proceedings]

73-20166575.2'1e-ISBN-13: 978-1-4613-4529-9

(Basic life sciences, v.3)Includes bibliographical references.1. Genetic transcription-Congresses. I. Biswas, B. B., ed. II. Title. [DNLM: 1.

Genetics, Microbial-Congresses. 2. Transcription, Genetic-Congresses. QH431 C144c1973]QH450.2.C34 1973ISBN-13: 978-1-4613-4531-2001: 10.1007/978-1-4613-4529-9

Proceedings of the Calcutta Symposium on Control of Transcription,February 12-15, 1973

© 1974 Plenum Press, New YorkSoftcover reprint of the hardcover 15t edition 1974A Division of Plenum Publishing Corporation227 West 17th Street, New York, N.Y. 10011

United Kingdom edition published by Plenum Press, LondonA Division of Plenum Publishing Company, Ltd.

Davis House (4th Floor), 8 Scrubs Lane, Harlesden, London, NW10 6SE, England

All rights reserved

No part of this publication may be reproduced in any form withoutwritten permission from the publisher

ORGANIZING COMMITTEE

Alexander Hol/aender-Oak Ridge National Laboratory, Oak Ridge, Tennessee,U.S.A., and University of Tennessee, Knoxville, Tennessee, U.S.A.

Sankar Mitra-Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A.Salil K. Niyogi-Oak Ridge National Laboratory, Oak Ridge, Tennessee, U.S.A.S. M. Sircar-Bose Institute, Calcutta, India.R. K. Mandai-Bose Institute, Calcutta, India.S. Ghosh-Bose Institute, Calcutta, India.B. B. Biswas, Convener-Bose Institute, Calcutta, India.

SPONSORS

Bose Institute, Calcutta 700009, India.Oak Ridge National Laboratory, Biology Division, Oak

Ridge, Tennessee 37830, U.S.A.United States National Science Foundation.Ministry of Science and Technology, Government of India.

ACKNOWLEDGMENTS

The following institutions and firms have extended their generous collaborationand assistance:

Rabindra Bharati University, Calcutta.United States Consulate, Calcutta.Toshniwal Brothers Pvt. Ltd., Calcutta.Systronics, Calcutta.Borosil Glass Works Ltd .. Calcutta.

Foreword

In numerous conversations with our colleagues from India, it was suggestedthat we help to institute a series of symposia in India similar in nature to thosethat have been conducted by our Latin American colleagues for more than 10years. We were fortunate to have with us in Oak Ridge Dr. Niyogi and Dr. Mitrafrom Indian universities. Their close ties with the Bose Institute in Calcutta andthe resultant correspondence with the Institute Director, Dr. S. M. Sircar,provided the stimulus for organization of this first Indian symposium, which washeld in Calcutta. Under the direction of Dr. Sircar, Dr. B. B. Biswas did anoutstanding job of organizing this conference.

Financial support was arranged through Dr. R. R. Ronkin of the UnitedStates National Science Foundation, who smoothed the way for the use of PL480 funds which were approved by the Indian Government for the organizationand running of this most valuable symposium.

The many Indian scientists who contributed papers and enthusiastically andvigorously entered into the discussions demonstrated the strength of modernscience in India. The topic, Control of Transcription, is a timely one, andconsiderable activity in this area is going on all over the world. The success ofthis symposium speaks well for the future of these Indian conferences andworkshops being planned for the next few years.

Again, the worldwide "community of science" is clearly manifested by theclose cooperation we have observed in this fruitful and successful symposium.

Alexander Hollaender

Biology DivisionOak Ridge National Laboratory

Oak Ridge, Tennesseeand

The University of TennesseeKnoxville, Tennessee

vii

Contents

IntroductionB. B. Biswas xiii

List of Participants xv

I. Fine Structure of Active Genes in Prokaryotes and EukaryotesBarbara A. Hamkalo and O. L. Miller, Jr 1

2. Changes in the Subunit Structures of Bacillus subtilis RNA Poly­merase During Sporulation

Richard Losick 15

3. Control of Transcription in Bacillus brevis by Small MoleculesHenry Paulus and Nilima Sarkar 21

4. Control of Transcription in Phage P22 Infected HostMaharani Chakravorty, P. S. Khandekar,G. R. Koteswara Rao, and Sushil Taneja .35

5. New Small Proteins Associated with DNA-Dependent RNA Poly­merase of Escherichia coli After Infection with T4 Phage

Audrey Stevens and R. Diane Crowder 53

6. The Stringent Response-21 Years OnAndrew Travers 67

7. Elucidation of RNA Initiation (DNA Promoter?) Sequences in T4DNA Transcription using Escherichia coli RNA Polymerase andDinudeoside Monophosphates

Salil K. Niyogi and David J. Hoffman 81

ix

x Contents

8. Stringent Coupling Between Transcription, Translation, andDegradation of Messenger RNA in Inducible Enzyme Systems: ATheoretical Analysis

U. N. Singh 93

9. Gene-Specific Changes in Induced Enzyme Synthesis in Escherichiacoli Infected with Phage </>X 174

Amit Ghosh and Ramendra K. Poddar 105

10. Initiation of Transcription by RNA Polymerases of Escherichia coliand Phage T3

E. K. F. Bautz, W. T. McAllister, H. Kilpper,E. Beck, and F. A. Bautz 115

11. Transcription of Native and Denatured DNA Preparations by Bac­teriophage T3 Induced RNA Polymerase

Umadas Maitra, Prasanta R. Chakraborty, Rino A. Salvo,Henry H. Huang, Pradip Bandyopadhyay, and Probir Sarkar 125

12. Physical Mapping of Transcribing Regions on Coliphage fd DNA bythe Use of Restriction Endonucleases

M. Takanami and T. Okamoto 145

13. Transcriptional Control of the Expression of a Degradative Plasmidin Pseudomonas

A. M. Chakrabarty 157

14. Studies on the Transcription of Simian Virus 40 and AdenovirusType 2

Joe Sambrook, Phillip A. Sharp, Brad Ozanne,and Ulf Pettersson .167

15. Transcription of Reovirus RNAAmiya K. Banerjee, C. Martin Stoltzfus, Richard L. Ward,and Aaron J. Shatkin 181

16. Initiation and Regulation of Transcription in Coliphage AWaclaw Szybalski 201

17. Termination and Antitermination in Transcription: Control of GeneExpression

Sankar Adhya, Max Gottesman, and Benoit de Crombrugghe 213

18. Control of Transcription in Neurospora crassaP. R. Mahadevan and A. S. Bhagwat 223

19. Transcriptional Systems in Eukaryotic CellsMichael Goldberg, Jean-Claude Perriard, Gordon Hager,Richard B. Hallick, and William J. Rutter 241

Contents xi

20. Transcription of Double-Stranded Viral and Cellular DNAs byPurified Mammalian DNA-Dependent RNA Polymerases

P. Chambon, J. L. Mandel, F. Gissinger, C. Kedinger,M. Gross-Bellard, and P. Hossenlopp 257

21. Factors Affecting the Selection of a Template for the Characteriza­tion of Multiple DNA-Dependent RNA Polymerases of MammalianTissues

Peter H. W. Butterworth, Sarah Jane Flint,and C. James Chesterton 269

22. RNA Polymerases and Controlling Factors from Plant Cell NucleiB. B. Biswas, H. Mondal, A. Ganguly,Asis Das, and R. K. Mandai 279

23. Restriction of RNA Synthesis by RNA Polymerase from AvianErythrocyte Nuclei

R. K. Mandai, Hemanta K. Mazumder, and B. B. Biswas 295

24. On the Regulation of Pre-mRNA Biosynthesis and TransportE. M. Lukanidin, O. P. Samarina, A. P. Ryskov,and G. P. Georgiev .303

25. The Poly(A) Sequences in Messenger RNA and HeterogeneousNuclear RNA

Hiroshi Nakazato and Mary Edmonds 317

26. Estradiol-Induced Expression of Genetic Information: Synthesis ofPhosvitin in Birds

G. P. Talwar, B. L. Jailkhani, M. L. Sopori, S. Venkatesan,A. Grover, P. R. Narayanan, and C. Narasimhan .333

27. Comparison of Biochemical Characteristics of Reverse Transcriptasefrom Human Acute Leukemic Cells and Several RNA TumorViruses

P. S. Sarin, J. W. Abrell, and R. C. Gallo .345

28. Synthesis by Reverse Transcriptase of DNA Complementary toGlobin Messenger RNA

lnder M. Verma, Gary F. Temple, Hung Fan,and David Baltimore .355

29. Investigations on Reverse-Transcribed DNA from RNA TemplatesM. R. Das .373

30. Transcriptional Control of M13 Phage DNA ReplicationSankar Mitra 383

xii Contents

31. Conversion of </>X174 and fd DNA to Their Replicative Forms byTwo Enzyme Systems in Escherichia coli

S. Wickner, R. B. Wickner, M. Wright, I. Berkower,and J. Hurwitz .395

32. A Quantitative Estimate of DNA· RNA Hybridization in Neuro­spora crassa

S. K. Dutta and D. R. Jagannath .411

Index 417

Introduction

In recent .years, a revolution has occurred in biology-a revolution yieldingbreakthroughs in many fields. The breakthroughs commenced when biologistsactually concentrated their attention on cells or cell organelles rather than onwhole organisms. Studies at the molecular level yielded much information on thebasic reactions of cells, and now studies are turning to the more complicatedsystems of differentiation and development. Certainly, a more unified molecularconcept in biology has developed, and the barrier between physics and chemistryand biology has gradually become lessened, a situation foreseen by J. C. Bose,the founder of this Institute more than 50 years ago.

Several years ago, plans were initiated to hold an international symposiumon modern biology at Bose Institute, Calcutta, India. In 1971, Drs. S. K. Niyogiand S. Mitra, who are at present working in the Oak Ridge National Laboratory,U.S.A., suggested to Professor A. Hollaender of the Oak Ridge National Labora­tory and The University of Tennessee that meetings be held in India similar tothose he so successfully organized in Latin America. Thereupon, Dr. S. M. Sircar,the Director of Bose Institute, contacted Professor Hollaender, who respondedso quickly that in I year's time the Symposium on Control of Transcription washeld in Calcutta (February 12-15, 1973). Professor Hollaender's untiring effortsand his experiences with the series of symposia in Latin America helped theorganization of this symposium immeasurably. The National Science Founda­tion, U.S.A., was contacted for financing the symposium through the Depart­ment of Science and Technology, Government of India. Both the governmentswere agreeable to allocate money from tied-up United States funds in India.

A number of international symposia on different subjects had already beenheld in this country, but most of them were of wider interest and thus exchangeof ideas between the scientists was rather limited. The specific aim of thismeeting was to discuss a more narrow topic so that a number of Indian scientistsfrom various disciplines could interact profitably. The purpose of the meeting

xiii

xiv Introduction

was also to attract a large number of the young scientists who will be responsiblefor developing modern biological trends in India as well as in other countries.The topic, Control of Transcription, was chosen with special consideration ofthe work going on in this Institute.

DNA-dependent RNA polymerase, the enzyme involved in transcription, i.e.,the copying of DNA molecules to yield RNA, was discovered more than adecade ago. Subsequent work showed many features of the reaction. However,when initiation and termination of specific RNA chains were looked into,additional protein factors were found to be involved. The mode of action andthe precise control exerted by these factors on RNA synthesis are being studiedat this time, and the overall process is certainly more complex than wasenvisaged. Studies of the role of transcription in DNA replication and the reversetranscriptase studies are also very exciting. The objective of arranging a Sympo­sium on Control of Transcription and including topics on (a) viral transcription,both DNA and RNA dependent, (b) prokaryotic RNA polymerases with theircontrolling factors, (c) transcription in eukaryotic systems, and (d) transcrip­tional control of DNA replication was to discuss the present status and thefuture development of the field. The outcome of this discussion is embodied inthis volume, prepared under the active advice of Drs. A. Hollaender, W. E. Cohn,A. Stevens, R. K. MandaI, and S. Ghosh.

The meeting left an excellent impression on the participants from India aswell as on those from other countries. I am sure that new ideas on the process oftranscription will evolve as a result of the meeting. It has been of tremendousvalue to the younger scientists from India who had never had a chance to attendthe same kind of meeting elsewhere. The symposium also has had an effect onthe public, because through the press and the radio the importance of transcrip­tion to medical and allied fields was emphasized. If symposia of this type areheld in India every 2 years, it will surely strengthen science in this country andestablish better human and scientific communication among scientists from allcountries.

Lastly, I want to acknowledge the help of Professors Hollaender and Sircar,the National Science Foundation, U.S.A., the Government of India, and allmembers of the organizing committee as well as the participants in making thesymposium a grand success. Plenum Publishing Corporation deserves specialthanks for processing the publication so efficiently.

B. B. Biswas

Bose InstituteCalcutta, India

List of Participants

INTERNATIONAL SYMPOSIUMON CONTROL OF TRANSCRIPTIONBose Institute, Calcutta, IndiaFebruary 12-15, 1973

Contributors

Adhya, Sankar-Laboratory of Molecular Biology, National Cancer Institute, NIH, Bethesda,Maryland 20014, U.S.A.

Banerjee, A. K. - Roche Institute of Molecular Biology, Nutley, New Jersey 0711 0, U.S.A.Bautz, E. K. F. -Molekulare Genetik der Universitat Heidelberg, 69 Heidelberg, Germany.Bhagwat, A. S.-Bhabha Atomic Research Centre, Trombay, Bombay-8S, India.Biswas, B. B. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Butterworth, Peter H. W. - Department of Biochemistry, London University, London, En-

gland.Chakrabarty, A. M -Physical Chemistry Laboratory, General Electric Corporation, P.O. Box

8, Schenectady, New York 12301, U.S.A.Chakravorty, Maharani-Department of Biochemistry, Institute of Medical Sciences, Banaras

Hindu University, Varanasi-S, India.Chambon, Pierre-Department of Biochemistry, Institut Chimie Bio1ogique, Faculte de

Medicine, Strasbourg, France.Das, M. R. - Tata Institute of Fundamental Research, Bombay-5, India.Dutta, S. K. -Department of Molecular Genetics, P.O. Box 105, Howard University, Wash­

ington, D.C. 20001, U.S.A.Edmonds, Mary-Department of Biochemistry, Faculty of Arts and Sciences, University of

Pittsburgh, Pittsburgh, Pennsylvania 15213, U.S.A.GaUo, R. C. -National Cancer Institute, NIH, Bethesda, Maryland 20014, U.S.A.Ghosh, S.-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Hamkalo, Barbara-Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

37830, U.S.A.HoUaender, Alexander-Biology Division, Oak Ridge National Laboratory, Oak Ridge,

Tennessee, and The University of Tennessee, Knoxville, Tennessee 37830, U.S.A.Kumar, Sushi/-Indian Agricultural Research Institute, New Delhi-12, India.

xv

xvi List of Participants

Losick, R.-Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138,U.S.A.

Lukanidin, E. M -Institute of Molecular Biology, Academy of Sciences, Moscow B-312,USSR.

Maitra, U. - Department of Developmental Biology and Cancer, Albert Einstein College ofMedicine, Bronx, New York 10461, U.S.A.

Manda/, R. K. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Mitra, S.-Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830,

U.S.A.Niyogi, S. K. -Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

37830, U.S.A.Paulus, H. -Department of Biological Chemistry, Harvard Medical School, Boston, Massachu-

setts 02115, U.S.A. ...Poddar, R. K. -Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta-37, India.Rutter, W. J. -Department of Biochemistry and Biophysics, University of California, San

Francisco, California 94122, U.S.A.Sambrook, Joe-Cold Spring Harbor Laboratory, P.O. Box 100, Cold Spring Harbor, New

York 11724, U.S.A. .. . .Singh, U. N. -Molecular Biology Unit, Tata Institute. (jfFundamental Research, Bombay,5,

India.Stevens, Audrey-Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

37830, U.S.A.Szyba/ski, Wac/aw-McArdle Laboratory, University of Wisconsin, Madison, Wisconsin

53706, U.S.A.Takanami, M -Institute for Chemical Research, Kyoto University, Kyoto, Japan.Ta/war, G. P. -All-India Institute of Medical Sciences, New Delhi-16, India.Travers, A. -MRC Laboratory of Molecular Biology, Cambridge, England.Verma, I. M -Department of Biology, Massachusetts Institute of Technology, Cambridge,

Massachusetts 02139, U.S.A.Wickner,· R. B. -Department of Developmental Biology and Cancer, Albert Einstein College

of Medicine; Bronx, New York 10451, U.S.A.

ObserVers

Bagchi, Buddhadev-Department of Physics, Calcutta University, Calcutta-9, India.Banerjee, A. B. -Department of Biochemistry, Calcutta University, Calcutta-19, India.B(lnerjee, S. -Central Drug Laboratory, Calcutta-16, India.Banerjee, Uma-Department of Physics, Calcutta UniversitY,Calcutta-9, India.Barua, A. K. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Basu, A. K. -Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta·9, India.Basu, Sandip-New York Public Health Research Institute, New York, New York 10011,

U.S.A.Bhadra, T. C. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Bhaduri, A mar-Department of Pharmacy, Jadavpur University, Calcutta-32, India.Bhargava, P. M. -Regional Research Laboratory, C.S.I.R., Hyderabad, India.Bhattacharya, Asoke-Department of Biochemistry, Calcutta University, Calcutta-l 9, India.Bhaftacharya, S. B. - Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta~37,

India.Biswas, (Mrs.) S. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Bose, S. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.

List of Participants xvii

Bose, S. K. -Department of Biochemistry, Calcutta University,Calcutta-19, India.Brahmachary, R. L. -Indian Statistical Institute, 203 Barrackpore Trunk Road, Calcutta-35,

India.Burma, D. P. -Bartaras Hindu University, Varanasi-5, India.Chakravorty, D.P. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Chakravorty, P.-Massachusetts Institute of Technology, Cambridge; Massachusetts 02139,

U.S.A.Chanda, (Mrs.) Rita-University of Rochester, Rochester, New York 14627, U.S.A.Chanda, S. K. -University of Rochester, Rochester, New York 14627, U.S.A.Chanda, Sunirmal-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Chatterjee, Asis-Director's Laboratory, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Chatterjee, A. K. -Department of Physiology, Calcutta University, Calcutta-9, India.Chatterjee, G. C. -Department of Biochemistry, Calcutta University, Calcutta-19, India.Chatterjee, I. B. - Department of Biochemistry, Calcutta University, Calcutta-19, India.Chatterjee, K. K. - Institute of Child Health, 95 Dilkhusa Street, Calcutta-l 7, India.Chatterjee, M. L. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Chatterjee, Rajyasree-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla

Chandra Road, Calcutta-9, India.Chatterjee. S. N. -Department of Zoology, Calcutta University, Calcutta-19, India.Chatterjee, Smriti N. -Department of Biophysics, Calcutta School of Tropical Medicine,

Calcutta-12, India.Chatterjee. Subhendu-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla

Chandra Road, Calcutta-9, India.Chaudhury. D. K. - Department of Biochemistry, Calcutta University, Calcutta-19, India.Chaudhury. Kanakendu-Chittaranjan National Cancer Research Centre, Calcutta-26, India.Chaudhury. Utpal-Department of Physics, Calcutta University, Calcutta-9, India.Choudhury, K. L. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Choudhury. Manas-Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chan-

dra Road, Calcutta-9, India.Das, Asis-Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.Das, H. K. - Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-12,

India.Das, J. -Department of Physics, Calcutta University, Calcutta-9, India.Das. Sunil K. -Department of Chemistry, Bose Institute, 93/1 Acharya Prafuila Chandra

Road, Calcutta-9, India.Das. Susanta-Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Das Cupta, Chanchal-Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta­

37, India.Das Gupta. Juthika-Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India. .Das Gupta. Shantanu-Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta-

37, India.Dass. C. M. S. -Delhi University, Department of Zoology, Delhi-7, India.Datta. Asoke G. - Indian Institute of Experimental Medicine, Calcutta-32, India.Datta. Lina-All-lndia Institute of Medical Science, New Delhi-16, India.Datta, Sahana-Director's Laboratory, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.

xviii List of Participants

Datta Gupta, A. K. -Department of Zoology, Calcutta University, Calcutta-19, India.De, Dilip-Department of Biochemistry, Calcutta University, Calcutta-19, India.Dutta, Asis K.-Division of Life Sciences, Jawaharlal Nehru University, New Delhi-53, India.Dutta, J. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Ganguly, Asok-Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Ganguly, Ranjan-Department of Zoology, Calcutta University, Calcutta-19, India.Ganguly, Subhendu-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Ganguly, Subinoy-Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Gayen, Samir-Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Ghora, B. K. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Ghose, A. M -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Ghose, Bharati-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Ghosh, Amit-Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta-37, India.Ghosh, H. P. -Department of Biochemistry, McMaster University, Hamilton, Ontario, Cana-

da.Ghosh, J. J. -Department of Biochemistry, Calcutta University, Calcutta-19, India.Ghosh, (Mrs.) K -Department of Biochemistry, McMaster University, Hamilton, Ontario,

Canada.Ghosh, N. -Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta-37, India.Ghosh, P. K. - Department of Biochemistry, Calcutta University, Calcutta-19, India.Ghosh, S. R. -Bose Institute, Calcutta-9, India.Ghosh, T. N. -School of Tropical Medicine, Chittaranjan Avenue, Calcutta-12, India.Ghoshal, Shamali-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla

Chandra Road, Calcutta-9, India.Guha, Chitrita-Indian Agricultural Research Institute, New Delhi-12, India.Gupta, R. -Physics Department, Calcutta University, Calcutta-9, India.Gurnani, Shantoo-Bhabha Atomic Research Centre, Trombay, Bombay-85, India.Kahn, Regina C -Institut de Biologie Moleculaire, 9 Quai Saint-Bernard, Paris Ve, France.Kanungo, MS.-Department of Zoology, Banaras Hindu University, Varanasi-5, India.Khan, N. C-Biophysics Laboratory, Saha Institute of Nuclear Physics, Calcutta-37, India.Kundu, Asit-Director's Laboratory, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

India.Lahiri Majumder, A. -Department of Botany, Biswa-Bharati, West Bengal, India.Maitra, (Mrs.) Shila-Department of Biochemistry, Calcutta University, Calcutta-19, India.Maity, I. B.-Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.Mazumder, B. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Mazumder, Hemanta K-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9,

India.Mazumder, P. -Saha Institute of Nuclear Physics, Calcutta-37, India.Mehrotra, N. N. -All-India Institute of Medical Science, New Delhi-16, India.Mishra, A. K -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Mishra, Tapan-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Mitra, B. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Mitra, Nivedita-Department of Zoology, Calcutta University, Calcutta-I 9, India.Mitra, Santosh -Chittaranjan National Cancer Research Center, Calcutta-26, India.Mondal, S. - Department of Zoology, Calcutta University, Calcutta-19, India.

List of Participants xix

Mandai, Sisir-Department of Zoology, Calcutta University, Calcutta-l 9, India.Mukherjee, B.B. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Mukherjee, K. L. -Institute of Child Health, Calcutta-I?, India.Mukherjee, (Mrs.) Manju-Department of Biochemistry, Calcutta University, Calcutta-19,

India.Mukherjee, Pranab-Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chan-

dra Road, Calcutta-9, India.Mukherjee, Subhas-Nil Ratan Sarkar Medical College, Calcutta-14, India.Murthy, Leelavati-Bhabha Atomic Research Centre, Trombay, Bombay-85, India.Nandi, P. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Neogy, R. -Chittaranjan National Cancer Research Centre, Calcutta-26, India.Netrawali, M. S. -Bhabha Atomic Research Centre, Trombay, Bombay-85, India.Palit, Pratip-Director's Laboratory, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.Palta, H. K. -Department of Botany, Panjab University, Chandigarh-8, India.Pradhan, D.S. -Bhabha Atomic Research Centre, Trombay, Bombay-85, India.Ranade, S. S. -Cancer Research Centre, Bombay-12, India.Roy, Deb Dutra-Department of Biochemistry and Biophysics, School of Medicine, Univer­

sity of California, San Francisco, California 94122, U.S.A.Roy, Gouranga-Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Roy, Jashodhara-Department of Chemistry, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Roy, Pampa-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Roy, S. C. -Department of Biochemistry, Calcutta University, Calcutta-19, India.Roy, Tapati-Director's Laboratory, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.Roychoudhury, R. -Indian Institute of Experimental Medicine, Calcutta-32, India.Sadhukhan, P. -Department of Physics, Calcutta University, Calcutta-9, India.Salahuddin, A. -Aligarh Muslim University, Aligarh, India.Samanta, H. -Saha Institute of Nuclear Physics, Calcutta-3?, India.Sarkar, P. K. -Department of Botany, Bose Institute, 93/1 Acharya Prafulla Chandra Road,

Calcutta-9, India.Sarkar, (Mrs.) Nilima-Department of Biological Chemistry, Harvard Medical School, Bos-

ton, Massachusetts 02115, U.S.A.Sarkar, S. -Institute of Muscle Research, Boston, Massachusetts 02109, U.S.A.Sen, A. -Bose Institute, 93/1 Acharya Prafulla Chandra Road, Calcutta-9, India.Sharma, A. K.-Department of Botany, Calcutta University, Calcutta-19, India.Siddiqi, O. - Tata Institute of Fundamental Research, Homi Bhabha Road, Bombay-5, India.Singh, Lalji-Department of Zoology, Calcutta University, Calcutta-19, India.Singh, S. K.-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Sinha, N. B.-Department of Microbiology, Bose Institute, 93/1 Acharya Prafulla Chandra

Road, Calcutta-9, India.Sircar, S. M -Bose Institute, 93(1 Acharya Prafulla Chandra Road, Calcutta-9, India.Sirsat, S. -Cancer Research Center, Bombay-12, India.Srivastava, L. M -All-india Institute of Medical Sciences, New Delhi-16, India.Sreenivasan, A.-Bhabha Atomic Research Center, Trombay, Bombay-85, India.Subrahmanyam. C. S. -Indian Agricultural Research Institute, New Deihi-12, India.Thakur, Asok-Department of Physics, Calcutta University, Calcutta-9, India.

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