CONTROL STRATEGY OF FMD IN LIBYA AND POST-VACCINATION MONITORING

Dr. Ibrahim EldaghayesFaculty of Veterinary Medicine

University of TripoliTripoli, Libya

ibrahim.eldaghayes@vetmed.edu.ly

Abdunaser Dayhum; Abdulwahab Kammon; Monier Sharif; Giancarlo Ferrari;

Keith Sumption; Donald King; Santina Grazioli and Emiliana Brocchi

Libya

Libya

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History of FMD in Libya:

Serotype Years

FMDV-O: 1959, 1962, 1967-1968, 1972, 1981-1983, 1988-1989, 1994, 2010-2013

FMDV-A: 1979, 2009

FMDV-C: 0000

FMDV-Asia 1: 0000

FMDV-SAT 1: 0000

FMDV-SAT 2: 2003, 2012

FMDV-SAT 3: 0000

Source: http://www.wrlfmd.org/

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Genotyping

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EuFMD Real-Time Training Course in Kenya (Jan 2013)

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Real-Time Training Course Lectures in Libya

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FMD Scientific DayTripoli, 30/03/2014

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Rapid Response Teams (RRT)

FMD lateral-flow devices: received from EuFMD

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The Progressive Control Pathway for FMD control

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• Progressive Control Pathway (PCP) and Risk Based Strategic Plan implementation:

Component 2.3 of EuFMD workplan (REMESA): Assist national FMD risk management as part of the REMESA action plan.

Week Date

Week 1 26-30/01/2014

Week 2 23-27/03/2014

Week 3 Postponed due to security reasons

Week 4 Postponed due to security reasons

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Mass Vaccination Campaign in LibyaSheep, Goats and Cattle: A & O

• Merial Vaccine• Strains: O Manisa – O 3039 ; A Iran 05; (SAT-2 in 2012)

• Killed and purified vaccine• 250 Vaccination Teams• Once per year (2012 & 2013)• 2014:

• Small ruminant once/year • Cattle twice/year

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Vaccine Matching

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Agreement between the Libyan government and IZSLER, Italy 2012

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Component 1 investigations in FMD suspected outbreaks.

Component 2 focused on the immune response of vaccinated animals.

Component 3 focused on serology as a mean to investigate on the level of FMD virus circulation (anti-NSP antibodies) and on the serotypes present (anti-SP serotype-specific antibodies).

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Component 1 (FMD suspected outbreaks – Clinical cases)

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COMPONENT 2 (immune response in vaccinated animals)

• This component has addressed the immune response of vaccinated animals.

• The outcome to be measured will be the level of antibodies before the vaccine is injected and the level of antibodies after 30 days from the date of vaccination.

• The animals (Cattle and small ruminants) selected for sampling were ear-tagged and blood samples were collected at the day of vaccination and repeated after 30 days.

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Component 2: Immune response in vaccinated animals

Sampling and testing criteria

Anti-NSP Ab and titration of SP Abs (serotypes O, A, SAT2) before and 30 days PV

For each species (goat, sheep, cattle): 150 animals, 6-12 month-old, ear-tagged

Possibly, 10 different owners for each species (15 animals for each owner)

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Immune response to vaccine in small ruminants farms

7 FARMS

Vaccine: type O+A

≈ 30 animals/farm

6 farms NSP-NEG

1 farm NSP-POS

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Immune response to vaccine in small ruminants

6 FARMS NSP neg

Vaccine: type O+A

≈ 30 animals/farm

SEROCONVERSION 30 DPV

Serotype O

135/164 (82%)

Serotype A

152/164 (93%)

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Immune response to vaccine in 1 cattle farm

Seroconversion 30 DPV

O A SAT 2

N + 30/30 30/30 29/30

% + 100 % 100 % 97 %

Vaccine: types O+A+SAT230 cattle, NSP neg

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Current workJan 2015 – Jan 2016

• Cattle: Collection of serum from unvaccinated animals at the time of injection of the vaccine (time 0) and tag the animals from which the sample has been collected (n=99).

• Collect serum after 2, 4 and 6 months.

• Booster vaccination at 6 months for 70 animals.

• Collect serum again after 2, 4 and 6 months.

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EuFMD Team (March 2015)

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Thanks to:• EuFMD

• IZSLER, Brescia and Ministry of Health, Italian Government

• FAO

• OIE

• REMESA

• Pirbright Lab

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