curriculum vitae - s3.amazonaws.com · 1. certified professor – nat taiwan univ sch med/ministry...

CURRICULUM VITAE (Update: 3/1/18)

PERSONAL

Name: Kwang Poo Chang 张光樸 Address: Sex: Male Dept. Microbiology/Immunology Birth date: November 12, 1942 Chicago Medical School/RFUMS Citizenship: Naturalized U.S. 3333 Green Bay Road Dependents: Chin Shen (Wife, Biochemist) North Chicago, IL 60064, USA Patrick (Son, born 1972) TEL 847-578-8837 FAX 847-578-3349 E-mail. [email protected]

EDUCATION:

B.Sc. 1965 Entomology/Plant Pathol, National Taiwan University, Taiwan M.Sc. 1968 Microbiology, University of Guelph, Ontario, Canada Ph.D. 1972 Cell Biology, University of Guelph, Ontario, Canada

TEACHING/RESEARCH POSITION:

Postdoctoral Fellow Oct. 1972 - Aug. 1974, Rockefeller University Research Associate Sept. 1974 - Feb. 1976, Rockefeller University Assistant Professor Mar. 1976 - Aug. 1979, Rockefeller University Associate Professor Sept. 1979 - Mar. 1983, Rockefeller University Professor (Tenured since 1984) April 1983 - Present, Chicago Medical School/UHS, FUHS,

RFUMS

TEACHING EXPERIENCE:

University of Guelph (1967-1971): INSTRUCTOR (Biology/Entomology/Zoology) Rockefeller University (1972-1982): LECTURER (shared responsibilities: Parasitology/Seminar) Chicago Medical School/UHS (1983-2007): LECTURER (shared responsibilities: Medical Microbiology

(Helminths, Protozoa, Entomology, Host-parasite Interactions); Molecular Biology (Molecular & Cell Biology Techniques); Molecular/Experimental Parasitology

Chicago Medical School/RFUMS (2007-Present): LECTURER (shared responsibilities): Medical Microbiology

(Ditto); Integrated Grad Program: (Molecular Techniques; 2007-present Molecular Cellular Biology II - RNA editing;

Method-Immunotherapy/vaccination) National Medical School Review (1996) Medical Parasitology

National Changhwa University, Taiwan (2011) GUEST LECTURER of Biotechnology Graduate School

(Biological/Biomedical Research lectures) 12/17/11-01/04/12 Jinan Medical University, Quanzhou, PRC (2012) GUEST LECTURER School of Medicine (Med. Parasitology/Graduate School Seminars) 03/03-29/12 National Taiwan University, Taipei, Taiwan (2012) GUEST LECTURER Schools of Natural Science/Medicine (Short-course-Special Topics/Res. seminars) 09/23-10/09/12

RESEARCH INTEREST: CELL BIOLOGY/MOLECULAR BIOLOGY/BIOCHEMISTRY OF PARASITISM & SYMBIOSIS IMMUNOLOGY (Immunotherapy/vaccination)

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KPChang CV (Con’t) AFFILIATED APPOINTMENTS: 1. CERTIFIED PROFESSOR – Nat Taiwan Univ Sch Med/Ministry of Education, Taipei, Taiwan 1989- 2. HONORARY PROFESSOR – Nat Inst Parasit Dis/Chinese Academy of Preventive Medicine, Beijing, 1996 3. VISITING PROFESSOR – Taipei Medical University, Taipei, Taiwan, 2013-2018 4. VISITING PROFESSOR – Laboratory of Parasitic Diseases, Xinjiang CDC, Urumqi, PR China, 2017-

HONORS/AWARDS: 1. PRESIDENT’S PRIZE for the best paper, Ann. Meet. Ent. Soc. Ontario, Canada, 1968 2. IRMA T. HIRSCHL CAREER SCIENTIST AWARD, New York City, 1977-1982 3. MORRIS E. PARKER AWARD Merit Sci Res, UHS/The Chicago Medical School, N Chicago, IL, 1985 4. SEYMOUR HUTNER AWARD, Society of Protozoologists, 1987 5. NIH INTERNATIONAL TRAVEL FELLOWSHIP - India-USA Science Exchange Program, Feb. 1992 6. VEL NAIR FELLOWSHIP – Rosalind Franklin University School of Graduate/Postgraduate Studies, 2005

UNIVERSITY COMMITTEE SERVICES: (University of Health Sciences=RFUMS/Chicago Medical School, 1983-present):

Radiation Safety (1986-2007) Histopathology Facility (Ad hoc, 1986) Electron microscopy (1990) Bio-analytical Methods (Ad hoc, 1986) Animal Resources (1989-1999) Patent (Ad hoc, 1983) Faculty Award (1992-2016) Faculty Affairs (1988) Biohazard (2000-2014t) Pharmacology Chair Search (1997) Grad Sch Appointment (Chair) (2013-) University Senate (1986-1987) Miscellaneous departmental Committees.

MEMBERSHIPS IN ACADEMIC SOCIETIES: American Society of Tropical Medicine & Hygiene 1977-2007 American Society of Microbiology 1976-1995; 2006-Present American Society of Parasitologists 1975-Present American Society for Cell Biology 1977-1993 American Federation of Tissue Culture Association 1979-1983 Royal Society of Tropical Medicine and Hygiene 1988-1990 Society of Protozoologists 1976-Present Society of Chinese Bioscientists in America 1986-present (Permanent member) Sigma Xi 1975-1977 New York Academy of Sciences 1975-1977 Parasitolgia Zoologica Academia Sinica 1987-Present

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KPChang CV (Con’t)

GRADUATE COURSES OFFERED: MB 5546 Research Techniques in Cell Biology (1983-2002)

This course is to acquaint students in the Department with the following techniques: tissue and microbial culture, electron microscopy, protein chemistry, hybridoma, FACS, OPFAGE, DNA/RNA techniques. etc. Some or all listed, or other related techniques, are offered, depending on the number and interests of students. Students are encouraged to work on their own subject matters of interest. Fall quarter. One hr of lecture and 3 hrs of lab/wk. (K.-P. Chang and departmental faculty).

MB 5547 Experimental Parasitology (1983-2005) Designed for students interested in basic research of parasitology, this course includes lectures, seminars and discussion with comprehensive coverage of the major tropical parasites currently under investigation. Students are to learn laboratory handling of Entamoeba, Leishmania, Plasmodium, Trypanosoma and other parasites as experimental models and their applications to resolve problems in tropical medicine and biology of parasitism in general. Spring quarter. Two hrs of lecture and three hrs of lab/wk.

MB 5520 Molecular Parasitology (Since 1983 as needed) This course involves conceptual and practical aspects of applying new biotechnology to studying parasites and parasitic mechanisms of major tropical diseases. Emphasis is placed on biotechnology in handling proteins, DNA and RNA. Students are to actively participate in searching and discussing current literature as well as in project-oriented research. Research topics are catered to the background, training and/or interests of the students. Winter quarter. 3 hrs/wk lecture or discussion. Completion of a research project and submission of final report required. Shared responsibilities with other faculty members.

MB5533 Departmental Seminar (1983-1987; 2006) Presentation of current research in Microbiology/Immunology by invited speakers, faculty and students. Required of all departmental students. Autumn, Winter and Spring quarters. One hr/wk. K.-P. Chang and faculty.

MB 5532 Departmental Journal Club (2002-2006)

Literature review by graduate students and faculty members. Articles selected by students and faculty members for evaluation and discussion. Students present and critique selected articles as well as serve as discussants. Autumn, Winter and Spring quarters. One hr/wk. K.-P. Chang and faculty.

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RESEARCH OBJECTIVES AND CURRENT PROGRAMS: Our current supports and emphasis are to develop photo-inactivated Leishmania genetically and chemically as non-viable, but delivery competent carrier for immuno-therapy and -prophylaxis against leishmaniasis and other infectious/malignant diseases.

My main research interest is to study molecular structures and biological functions of extrachromosomal self-replicating entities in eukaryotic cells. Biological models studied include parasitic protozoa in mammalian cells, endosymbiotic bacteria in insects and protozoa, and extrachromosomal circular DNA molecules amplified from chromosomes in response to drug selection. Emphasis is placed on the understanding of molecular integration and discordance of intracellular interactions. Experimental approach is multidisciplinary.

My work supported by NIH has been focused on the Leishmania model for molecular mechanisms of microbial virulence. Specific infection-related molecules are invasins/evasins (invasive/evasive determinants) of the parasites, e. g. zinc protease/major surface glycoprotein (gp63), microsomal glycosyltransferase in the dolichol pathway of protein glycosylation and the ATP scavenging enzyme, e. g. nucleoside diphosphate kinases. Gp63 expression is regulated posttranslationally by N-glycosylation, which is in turn controlled by the glycosyltransferase gene expression. Secretory nucleoside diphosphate kinase is under study as an evasive determinant to prevent ATP-induced cytolysis and P2X7-mediated apoptosis of macrophages.

My past and current research includes the following specific topics: (1) Molecular Biology: Structure, regulation and expression of virulence and virulence-regulating genes cloned from Leishmania DNA studied by molecular genetic approach, i. e. gene replacement and transfection; (2) Biochemistry: Purification and characterization of Leishmania gp63, microsomal N-acetylglucosamine-1-phosphate transferase and the ATP scavenging enzymes; (3) Cell Biology: Post-translational regulation of these molecules and their activities in intracellular parasitism of macrophages by Leishmania; and (4) Molecular immunology: Genetic analysis of immunologically active parasite epitopes as the pathogenic determinants responsible for the clinical symptoms (=virulence) manifested in human leishmaniasis. Prophylactic and diagnostic potentials of these epitopes targeted for preparing attenuated mutants as vaccines and recombinant products as antigens for diagnosis, prophylaxis and therapy; (5) Molecular epidemiology: Genetic/genomic polymorphism of Leishmania single-copy/multicopy genes in relation to their evolution and virulence in clinico-epidemiology.

My overall long-term research interests include: (1) Evolution and function aspects of bacterial endosymbiosis in crithidia spp.; (2) Mitochondrial DNA transkinetoplastidy – A novel phenomenon involving mitochondrial or kinetoplast minicircle DNA amplification/deamplification; (3) Expression vector designs for Leishmania specific gene over-expression and knockdown; (4) Leishmania model for microbial virulence and human porphyria; (5) Development of Leishmania mutants as a universal platform for vaccine and drug delivery against infectious and malignant diseases.

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KPChang CV (Con’t) PATENTS 1. US patent No. 7,238,347 B2, July 3, 2007 Chang, KP, Kolli, BK, et al. Suicidal mutant Leishmania vaccine 2. US patent No. 7,261,887 B2, August 28, 2007 Chang, KP, Sassa, S, Sah, JF, Ito, H, Kolli, BK and Peterson, DA Leishmania as carriers for the delivery of proteins and peptides 3. US patent No. 9,327,017, May 3, 2016 (filing date: May 10, 2012) Chang, KP and Dutta, S. Leishmania-based carrier for vaccine delivery 4. New Patent application Ser, No. 60/204,188 filed Aug. 11, 2016 Chang KP, Kolli BK, Ng KPD, Photosensitized trypanosomatids and vaccine composition thereof. 5. New Patent application Ser. No. 62/323.064 filed April 10, 2017 Chang KP, Kolli BK, Shiao SH

Photodynamic insecticides

6. Provisional patent application#: 62502214 filed May 5, 2017 Chang KP, Kolli BK, Corso R, Manna L Immunotherapy of canine leishmaniasis

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KPChang CV (Con’t) GRANTS/AWARDS: Current support* National Institutes of Health (NIAID) Direct costs

1. Research Grant AI-11916 (1974-1978) Co-P.I. $151,418 Host-parasite membrane interactions in Leishmania (P.I. W. Trager) 2. Research Grant RO1 AI-15183 (=20486) (1978-1981) P.I. $128,835 Leishmania-macrophage cellular interactions in vitro 3. Research Grant RO1 AI-20486 (1982-1985) P.I. $289,926 Leishmania-macrophage cellular interactions in vitro 4. Research Grant RO1 AI-20486 (1985-1989) P.I. $480,885 Leishmania-macrophage cellular interactions in vitro 5. Research Grant RO1 AI-20486 (1989-1994) P.I. $796,111 Leishmania-macrophage cellular interactions in vitro 6. Research Grant RO1 AI-20486 (1995-2001) P.I. $634,449

Leishmania-macrophage cellular interactions in vitro

7. Research Grant RO1 AI-20486 (2002-2006) P.I. $700,000 Leishmania-macrophage cellular interactions in vitro

8. Research Grant R21 AI-68835 (2006-2008) PI $275,000 Transgenic porphyric Leishmania as suicidal live vaccines against leishmaniasis 9. Research Grant R21AI-7712375 (2008-2011) PI $275,000 Toward suicide automation of porphyric Leishmania For photodynamic vaccination 10. *Research Grant R21AI097830-01A1 (2012-2014) PI $275,000 Photo-inactivation of Leishmania for safe and effective delivery of surrogate vaccine No cost extension to 2016 11. Research Grant R21AI 11066421 (2012-2014) PI $275,000 Novel tools for Leishmaina stage-specific gene expression Knockdown (Pending resubmission) 12. STTR Sub-award Tracking #11543714 (2015-2016) $ 60,000 Development of photo-inactivated Leishmania as vaccines/vaccine Carriers (Pending resubmission)

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KPChang CV (Con’t) GRANTS/AWARDS: (Con’t) National Science Foundation

1. Research Grant PCM-8207554 (1983-1985) P.I. $115,000 Intralysosomal symbiosis of Leishmania with macrophages 2. Supplemental to #1 PCM-8309895 (1985-1986) P.I. $5,750 3. NATO Workshop Supplemental Award (1986) $5,375

(PCM-8608353) American Heart Association (Greater Midwest) Grant-in-Aid AHA ID # -755667Z (2007-2009) P.I. $120,000 Porphyric Leishmania as inducible suicidal mutants to Present Trypanosoma cruzi T-cell epitopes in vitro World Health Organization Special Program for Research & Training in Tropical Diseases

1. Research Contract N0. 780040 (1978-1980) P.I. $81,914 Nutritional aspects of intracellular parasitism in leishmaniasis 2. Research Contract N0. 350305 (1985-1988) P.I. $44,690 Monoclonal purified surface gp63 for vaccination and diagnosis of leishmaniasis 3. Research Subcontract N0. 950512 (1995) P.I. $30,000 Transfection of Leishmania for vaccination of helminthiasis

Rockefeller Foundation

1. Research Grant GA HS 8230 (1982) P. I. $10,000 Vaccination in murine leishmaniasis and host immune response (To support visiting scholar Dr. Xiao-su Hu) 2. Research Grant GA HS 8309 (1984) P.I. $6,500 Biology of leishmaniasis in the People’s Rep. of China

Other Sources & miscellaneous 1. Hirschl Award (1977-1981) P.I. $75,000 Intracellular parasitism and symbiosis in trypanosomatids 2. Engelhardt’s Fund (1981-1982) P.I. $2,000 Supplementary support for Dr. Xiao-su Hu 3. GCA Corporation Grant (1984-1985) P.I. $25,000 Application of Zimmerman Fusion System to cell biol. research 4. NATO ARW Award (1986) Director B.Fr. 1,155,000 Host-Parasite cellular and molecular interactions in protozoal infection. Advanced Research Workshop, Acquafredda di Maratea, Italy 5. Monbusho Consultant (l995-1998) (P.I. = Y. Matsumoto) ~$1,000,000 (To YM)

Leishmania and leishmaniases in Central Eurasia

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KPChang CV (Con’t) GRANTS/AWARDS: (Con’t)

6. DRR-BRS Shared Instrumentation Grant (1984-1985) Co-P.I. $237,390 Protein structure analysis in research in parasitology/ Tropical medicine (P.I.: R.G. Kemp)

7. BRSG, UHS/Chicago Medical School (1985, 1988, 1989) P.I. $9,000 Leishmania-macrophage metabolic interactions in vitro 8. University Pilot Study: Photodynamic vaccination (2004-6) P.I. $25,000 (2007-8) P.I. $25,000 9. *NBST (KSA National Plan for Science, Technology and Innovation $200,000 12-MED 2681-02 (Co-PI) (2014-present) Photo-inactivation of Leishmania tropica for prophylaxis and therapy (PI = Saad H. Alkahtani, King Soud University, Riyadh, SA) 10 *Burdine Breast Foundation/Cancer Treatment & Research Center $100,000

“Novel strategy of immunotherapy for treatment of malignant diseases” PI (2015-present)

11. Private donation $100,000 12. UK Innovate/Health Dept Subcontract (PI = Dr. GL Fu, GeneFirst (2017-) £ 63,000

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PROFESSIONAL RESEARCH PERSONNEL: Postdoral Fellows/Research Associates:

Subsequent or current Position: 1980-1982 Dunne Fong, Ph.D. Associate Professor (Research Associate) Department of Biology Rutgers University Piscataway, New Jersey 1981-1983 Xiao-Su Hu, M.D. Emeritus Professor, Dept. of Parasitology (Visiting Scholar) Northwest China University of Medical Science Chengdu, Sichuan, P.R. China 1983-1985 C.S. Chang, Ph.D. Manager (Res. Assistant Professor) QC/QR and R & D Merial Co. (Merck/Avantis) North Brunswick, NJ 1984-1985 V. Prachayasittikul, Ph.D Head/Dean (Postdoctoral Fellow) Faculty of Medical Technology Mahidol University Bangkok, Thailand 1985-1987 S. Detke, Ph.D. Associate Professor (Res. Assistant Professor) Dept. Biochemistry/Mol. Biology University of North Dakota Grand Fork, North Dakota 1985-1986 Yan Peng Research Assistant (Visiting Fellow) Pharmacology & Molecular Biology FUHS/CMS 1987-1989 Ran Zhang, Ph.D. Scientist (Visiting Fellow) Institute of Endocrinology National Institutes of Health Bethesda, MD 1986-1989 Ken Katakura, Ph.D. Professor (Research Associate) Laboratory of Parasitology, Department of Disease Control, Grad. Sch. of Vet. Medicine, Hokkaido University, Hokkaido, Japan 1985-1990 Gautam Chaudhuri, Ph.D. Professor/ Director of Mol Biology (Res. Assistant Professor) Meharry Medical College Nashville, TN 1986-1990 Minu Chaudhuri, Ph.D. Associate Professor (Research Associate) Meharry Medical College Nashville, TN 1990-1991 Carl P. Bergstrom, Ph.D. Department of Discovery Chemistry, (Postdoctoral Fellow) Research and Development, s Bristol Myer

Squibb, Wallingford, CT

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PROFESSIONAL/ESEARCH PERSONNEL: Postdoral Fellows/Research Associates: Subsequent or current Position 1991-1991 Dwight V. Nissley, Ph.D. Principal Scientist/Scientific Program Manager (Postodoctoral Fellow) NCI-Cancer Research & Development Center At SAIC-Frederick, MD 1991-1991 Sibaji Sarkar, Ph.D. Postdoctoral Fellow (Postdoctoral Fellow) Dept. Biochemistry & Molecular Biology

University of Chicago Chicago, IL

1991-1992 Hasan Aksu, M.D./Ph.D. Professor (Visiting Fellow) Dept. Clinical Bacteriology & Infectious Diseases Cukurova University Medical Faculty Adana, Turkey 1992-1993 M. Masoom Yasinzai, Ph.D. Deputy Director (Fulbright Fellow) Institute of Biochemistry University of Baluchistan Quetta; and Vice President, Quaid-e-Azam University, Islamabad, Pakistan 1991-1994 Honggang Lu, M.D./Ph.D. Director of Clinical Pathology (Postdoctoral Fellow) Juliet Hospital Juliet, IL;

Anatomic Pathology & Clinical Pathology - Hayward, CA

1993-1994 Shin- I. Kawazu, D.V.M./Ph.D. Professor (Postdoctoral Fellow) National Research Center for Protozoan

Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaidō, Japan

l993-1994 Li Zhong, M.D. Res. Associate (Visiting Fellow) Department of Pathology University of Wisconsin Madison, WI l993 Jing-Qi Qu, M.D. Senior Investigator (Visiting Scholar) Institute of Parasitic Diseases Chinese Academy of Preventive Medicine Shanghai, PR China 1994 Sarman Singh, M.D. Professor/Chief (Fellow of Indian Government) Laboratory Medicine All India Institute of Medical Sciences New Delhi, India 1994-1995 Rong-Qi Wang, Ph.D. Research Associate (Visiting Fellow) Research Department Evanston Hospital Northwestern University Evanston, IL

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PROFESSIONAL/ESEARCH PERSONNEL: Postdoral Fellows/Research Associates Subsequent or current Position: 1994-1995 Hiroya Ito, D.V.M./Ph.D. Investigator (Visiting Fellow) Laboratory of Bacteriology National Institute of Animal Health Tsukuba, Japan 1996 Dinh Tao Nguyen, M.D./Ph.D. Investigator (Fellow of US/Vietnam Found.) National Institute of Burn and Center for Cancer Research Hanoi, Vietnam 1996 Seray Osenzoy, M.D. Professor (NATO Res. Fellow) Dept. Parasitology Ege University Faculty of Medicine Izmir, Turkey 1996-1997 Mohamed Fakruddin, Ph.D. Research Fellow (Postdoctoral Fellow) Department of Medicine Columbia University New York, NY 1996 - 1998 Deqiao Chen, Ph. D. Research Associate (Postdoctoral Fellow) Research Department Evanston Hospital Northwestern University Evanston, IL 1997-1997 Nagendra Yadava, Ph.D. Associate Scientist (Postdoctoral Fellow) Department of Cell Molecular Biology University of California, San Diego, CA;

Scientist at Pioneer Valley Life Sciences Institute; Springfield, MA

1998-2000 Jerome Franklin Sah Research Associate (Postdoctoral Fellow) Case Western Reserve University Cleveland, OH 2000-2001 Neeloo Singh Scientist Department of Mol Biology, Div. Biochemistry Central Drug Research Institute Lucknow, India 2001 Carlos Muskus Programa de Estudio y Control de

Enfermedades Tropicales-PECET, Universidad de Antioquia, Medellín, Colombia

2009-2010 Ishita Chatterjee Postdoctoral Fellow (Postdoctoral Fellow) School of Medicine University of Illinois, Chicago, IL 2010 Esin Ayden Clinical Fellow (Summer Fellow) Microbiology/Immunology Faculty of Medicine Mersin University Mersin, Turkey

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PROFESSIONAL RESEARCH PERSONNEL: Postdoral Fellows/Research Associates Subsequent or current Position: 1999-2012 Bala Krishna Kolli Senior Research Associate (Senior Research Associate) Clinical Immunology Dept Microbiology/Immunology Chicago Medical School/RFUMS

North Chicago, IL 2007-2012 Sujoy Dutta Research Assistant Professor (Senior Research Associate) Cancer Research Program Dept Microbiology/Immunology Chicago Medical School/RFUMS North Chicago, IL 2013-2014 Pankaj Tripathi University faculty member Lucknow, India 2013-2014 Meral Miraloglu Associate Professor Dept of Clinical Microbiology Tropical Medicine Clinic Cukurova University School of Medicine Adana, Turkey 2014- Bala Krishna Kolli In residence

Graduate Students : 1983-1985 Julia A. Kiehlbauch (PhD) Director (Joint Advisor) Clinical Bacteriology New York State Department of Health Axelrod Institute Albany, NY 1983-1987 John Alan Kink (PhD) Section Manager Infectious Disease Group Ophidian Pharmaceuticals Madison, WI 1988-l993 Xuan Liu (PhD) Research Scientist/Marketing President OriGene Technologies, Inc. Rockville, MD 1989-1994 Yubin Du (PhD) Research Scientist Laboratory of Immunopathology NIAID, NIH Bethesda, MD 1989-1995 Min Huang (PhD) (Joint Advisor) Clinical Pathology Fellow Pathologist Fox Chase Cancer Center Philadelphia, PA 19111-2497

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KPChang CV (Con’t) PROFESSIONAL/ESEARCH PERSONNEL: Graduate Students : Subsequent or current Position: 1991-1995 Bradford S. McGwire (MD/PhD) Associate Professor Department of Microbiology/Internal Medicine Ohio State University, Columbus, OH 1991-1996 Mary Ellen Palko (MSc) Research Staff NCI-Cancer Research & Development Center Frederick, MD 1995-1998 Leyla Akman Anderson (MD/PhD) Assistant Project Scientist Dept. Medical Microbiology and Immunology University of California Davis, CA 2003-2005 Ai-Hua Tang (MSc) PhD candidate University of Mississippi Jackson, MS 2003-2005 Debalina Ray (MSc) PhD candidate University of Illinois Normal, IL 2001-2006 Kayoko Waki (Ph.D.) Postdoctoral Associate HIV Drug Development Programs NCI, Frederick, MD 2002-2007 Sujoy Dutta Research Assistant Professor Cancer Research Program Dept Microbiology/Immunology Chicago Medical School/RFUMS N Chicago, IL 1983- Short-Term lab rotation/Summer Research Fellows:

J Ahmed, L Berstrom, A Halepota, FY Liu, XJ Wang, R Pithawalla, RM Soetikno, T Singh, Z Chen, XH Yang, QW Zeng, Paula Cho, Johanna Schneider, Jun Liu, Howard Liu, Jared Nielson, Qian Zhang, Yi Ja, Reha Toydemir, Monica Nordstrom, Ken Henrick, Drugyn Racine, Falcon Chu, Nagaraj Kurei, Emmie Liang, Rohit Loomba, Bryan Kratz, Gabriela Sciser, Brice Jones; Daniel Proctor, Chris Simokat, Richard Lichangco, Andrew Surro, Benjamin Perea, Emily Harris, Anna Zurek, Kate Marichal, John Tang, Vikram Thanigaivelan.

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ACADEMIC, ADVISORY & CONSULTANT SERVICES:

Grant Proposal Reviewer:

1984-1989 U.S. Army Med. Res. Development Command, Parasitic Diseases Review Panel 1987-1991 U.S.-NIH Tropical Medicine & Parasitology Study Section 1979- Ad Hoc U.S.-Israel Binational Science Programs 1980- Ad Hoc National Science Foundation 1982- Ad Hoc U.S.-NIH-NIAID Ad hoc program committees 1983- Ad Hoc Canadian Medical Research Council 1986- Ad Hoc Montanans on a New Trac for Science 1987- Ad Hoc Wellcome Trust, London, UK 1988- Ad Hoc British Columbia Health Research Foundation 1991- Ad Hoc Veterans’ Administration 1993 NIH-RFP-OTMIR 93-27 Special Review Committee member 1996-1998 Scientific Review Committee, National Health Research Institute, Taiwan 1997-1998 Hong Kong University Grants Committee 1997 The Science Foundation of Israel 1997-1998 Hong Kong City University Grant reviewer 1998-2001 U.S. Army Medical Research/Infectious Disease Programs: AIBS Review Panel 2000 NIH-FIC, SEP, International Clinical Operational Health Research Training Award

(ICOHRTA) 2001 NIH, FIC, SEP, Global Infectious Disease Research Training Program Award 2002 NIH-FIC, SEP, ICOHRTA 2003 NIH-SEP for AOIC, ZRG1 AARR-A (04) 2006 NIH-Immunity and Host-Defense (IHD) Study Section. 2006 NIH-Tropical Medicine Research Center Study Section 2006 NIH-International Research in Infectious Diseases Study Section 2008 NIH-Immunity and Host-Defense (IHD) Study Section 2008 NIH-RFA AI-008-005. Development of novel interventions and tools for the

control of malaria, neglected tropical diseases (NTDs) and their vectors 2009 NIH-ARRA Challenge Grant Reviews: ZRG1 IMM-E 2010 NIH ZRG1 Pathogens/Symbionts SEP-IDM-B (02) 2010 NIH-RPG (RO1/R03/R15/R21) review (8/2/10) 2010 Wellcome Trust Intermediate Fellowship 2010 US-Israel Binational Science Foundation BSF 2009 grant applications 2011 NIH-2011/05 ZRG1 BCMB-A (51) R Transformative R01 Roadmap (04/06/2011) 2011 NIH- Tropical Medicine Research Center review panel member (11/11) 2012 NIH-ZAI1 JTS-M M1 1, NIAID Clinical Trial Planning/Implement. (2/6-7/12) 2012 NIH-ZAI1 RRS-M M4 1, Host Targeted Interventions as Therapeutics For

Infectious Diseases (3/13/12) 2014 US-NIH- IDM-M (02) “Vectors and Pathogens”, Internet review, October 22-23. 2014 US-NIH-NIAID, Int. Collab. Inf. Dis. Res. (ICIDR), Washington, DC Aug. 26-27. 2015 Taiwan-Min. Sci. Technol. -Emerging Inf. Dis. (EID), San Francisco, CA, Dec.

15-16, 2014 2015 UK-MRC (Medical Research Council) “UK-Brazilian Translational Collaborative

Research Programs” Aug., 2015.

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KPChang CV (Con’t) CONSULTANT/INSTRUCTOR/ORGANIZER: 1978, Nov. Member Official Delegation of the American Society of Tropical Medicine & Hygiene to visit PR China 1978-1981 Consultant WHO contract “Polyamine as drug targets on Trypanosoma brucei” (P.I. C.J. Bacchi, Pace University) 1979, Aug. Instructor WHO Malaria parasite in vitro culture training course, Shanghai, PR China (Director: William Trager) 1980-1981 Member Advisory Committee on rickettsia section of Bergey’s Manual of Determinative Bacteriology 1982, Mar. Consultant UNDP-TOKTEN Programme-Biomedical Research, Zhongshan University, Guangzhou, PR China 1983, Aug. Instructor Biology of Parasitism summer course, Marine Biological Laboratory, Woods Hole, Massachusetts 1984, Sep. PI Leishmania and Leishmaniasis in China, Rockefeller Foundation Research Project in PR China 1986, Sep. Organizer NATO Advanced Research Workshop: Host-Parasite cellular and molecular interactions, Aquafredda di Maratea, Italy 1988, May Consultant WHO Special Programs, Institute Strengthening, Shandong Institute of Parasitic Diseases, Jinin, Shandong, PR China 1988-1989 PI Molecular Parasitology Programs, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan 1988- Adj. Professor Dept. Parasitology, National Taiwan University School of Medicine, Taipei, Taiwan. 1992 Coordinator Fogarty International Health - Indo-USA Exchange Programs, Int. Symp. Leishmaniasis Research, IICB, Calcutta, India 1995 Organizer Leishmania Cell and Molecular Biology Round Table Session, VIII International Congress of Parasitology, Izmir, Turkey 1996 Organizer Leishmaniasis Symposium, Glob Meet Parasitic Diseases, New Delhi, India 1996 Organizer Leishmania Molecular Biology Symposium, 14th International Congress of Tropical Medicine & Malaria, Nagasaki, Japan 1996 Co-organizer Leishmania State-of-The-Art Session, 14th International Congress of Tropical Medicine & Malaria, Nagasaki, Japan 1997 Member Organizing Committee, Scientific Programs, World Leish I, Istanbul, Turkey 2000 Organizer Scientific Committee, WorldLeish II, Krete, Greece 2005 Chair Scientific Committee, WorldLeish III, Sicily, Italy 2004-05 Consultant Ford Dodge Animal Health, Iowa

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KPChang CV (Con’t) CONSULTANT/INSTRUCTOR/ORGANIZER: 2009-11 Chair Scientific Committee, WorldLeish IV, Lucknow, India 2009 Invited Consultant “Polymer nanotechnology as Leishmania vaccine adjuvant carriers”, TUBITAK

Project (The Scientific and Technological Research Council of Turkey), Bio-engineering Department, Davupasa Campus, Yildiz Technical University, Istanbul, Turkey, November 5-12, 2009.

2013-15 Chair Scientific Committee, WorldLeish V, Recife, Brazil. 2015 Commentator NIH-NIAID Workshop “Control and elimination of visceral leishmaniasis: Does

vaccination have a role ?, Rockville, MD, September 9-10. 2015-17 Chair Scientific Committee, WorldLeish VI, Toledo, Spain, May 20-26, 2017. 2016 Member Bayer GVS Animal Health Advisory Board Meeting, Miami, FL, June 10th.

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PROFESSIONAL SERVICES - EDITOR OR REVIEWER: Book Editor:

1. “Malaria and Other Protozoal Infections”, Zhongshan University Press, Guangzhou, People’s Rep. of China, 1982.

2. “Leishmaniasis” in “Human Parasitic Diseases” series, Elsevier/North Holland, Amsterdam, 1985. 3. “Host-Parasite Cellular and Molecular Interactions in Protozool Infection”, NATO ASI Series, Springer-Verlag, Heidelberg, 1987. 4. Molecular Biology International, Associate Editor for Special volume “Target identification and intervention strategies against kinetoplastid protozoan parasites”, 2011. 5. Journal of Parasitology Research “Molecular epidemiology and parasite genetic heterogeneity in leishmaniasis”, 2016. MEMBER OF EDITORIAL BOARD: 1. International Journal Endocytob. Cell Res. 1984-1999 2. Experimental Parasitology 1987-2001 3. Journal of Eukaryotic Microbiology 1988-2004 4. Memoir Fund. Oswaldo Cruz 1994- 5. Journal of Parasitic Diseases 1996- 6. Current Drug Targets-Infectious Disorder 2001-2003 7 Parasitology International 2001- 8. Clinical Medicine: Pathology 2007- 9. Journal of Parasitological Research 2010- (Asso. editor “Immunity to protozoan parasites”) 10. Journal Immunology/Immunotherapy 2014- (In progress) 11. JSV Microbiology 2015- 12. Int. Journal of Vaccine/Immunization 2015- 13.Global Vaccine & Immunology 2015- 14. J Vaccines Res & Vaccination 2017- 15. Ann Agricultur & Crop Sci 2017- PERIODICAL JOURNAL REVIEWER: Antimicrobial Agents and Chemotherapy International Endocytobiosis and Cell Research Archives of Biochemistry & Biophysics Journal of Biological Chemistry American Journal of Tropical Medicine and Hygiene Journal of Crude Drug Research Biochemical Medicine and Metabolic Biology Journal of Eukaryotic Microbiology Biochemical Pharmacology Journal of Immunology Biology of the Cell Journal of Infectious Disease BMC Veterinary Research Infection and Immunity Canadian Journal of Microbiology Journal of Parasitology Cell Molecular Biology Report Journal of Virology Chemistry and Biology Life Sciences Clinical Infectious Diseases Molecular and Biochemical Parasitology Clinical and Vaccine Immunology PLoS Neglected Tropical Disease Infectious Diseases of Poverty PLoS ONE CRC Press Molecular Pharmaceutics Eukaryotic Cells Nucleic Acids Research European Journal of Biochemistry Parasitology European Journal of Cell Biology Parasitology International Experimental Biology and Medicine Parasitology Research Experimental Cell Research Proceeding National Academy of Sciences USA

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Experimental Parasitology Science FEMS Trends in Biochemical Sciences Trends in Parasitology Gene Z. Parasitunkende Int Journal of Parasitology

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INTERNATIONAL AND NATIONAL SYMPOSIA - INVITED SPEAKER/CHAIR: 1974 Symposium Society of Experimental Biology No. 29, Bristol, U.K. 1977 V International Congress of Protozoologists, Symposium “Endosymbiosis in trypanosomatid flagellates”, New York, N.Y. 1979 Plenary Session, British Society of Parasitology Annual Meeting, University of Keele, Stoke-On-Trent, U.K. 1979 WHO Workshop on in vitro cultivation of pathogens of tropical diseases, Feb. 4-9, Nairobi, Kenya. 1980 Jenssen Research Foundation Workshop on host-invader interplays, July, Beerse, Belgium. 1981 Gordon Research Conference “Intracellular parasitism in leishmaniasis”, Aug., New Hampshire. 1982 International Congress for Microbiology, Symposium on obligate intracellular parasites, Boston. 1982 Ciba Symposium No. 99, Pathobiology of parasitic diseases, “Cell biology of host-parasite membrane interactions in leishmaniasis”, Caracas, Venezuela 1983 2nd International Colloquim on Endocytobiology,. “Intralysosomal symbiosis in leishmaniasis”, Tubingen, Germany (Chairman) (Declined). 1983 Marine Biological Laboratories General Scientific Meeting, Woods Hole, MA (Chairman). 1984 XI International Congress for Tropical Medicine & Malaria, Calgary, Canada (Speaker and Co-Chair). 1984 IV Mediterranean Multicolloquim Parasitology, Izmir, Turkey. 1984 II Congress Iberam. Histoquim Cytoquim-Microscop. Electron, Maracaibo, Venezuela (Declined). 1985 VII International Congress Protozoologists, Symposium on Phagocytosis of and by protozoa, July, Nairobi, Kenya.

1985 Br./Scand. Joint Meeting Trop. Med. Parasitology, Sep., Copenhagen, Denmark (Declined). 1985 LEISHMANIASIS: The first centenary, Nov., Royal Army Medical College, Millbank-on-the-Thames, London, UK (Declined). 1985 Application of molecular biology to studies of Leishmania - Specialist Meeting, December 3-6, Cotswold, Worcestershire, UK (Speaker and Chair). 1986 1st SCBA International Symposium and Workshop, June 28-July 1, San Francisco. 1986 NATO Advanced Research Workshop “Host-Parasite Molecular Interactions, Sept., Acquafredda di Maratea, Italy (Director).

20


INTERNATIONAL AND NATIONAL SYMPOSIA: INVITED SPEAKER/CHAIR: (CON’T) 1986 Leishmaniasis Workshop, Cent. Nacional Microbiol., Virol., Immunol. Sanitarias, Inst. Nac. Sanidad, October, Madrid, Spain. 1987 Jornadas Internacion Parasitol. Enfermed. Parasit., Fund. Romon Areces, May, Madrid, Spain. 1987 1st Midwest Tropical Diseases Research Meeting, University of Illinois School of Medicine, March, Rockford, Illinois. 1987 Symposia of Advanced Biological & Medical Sciences, Academia Sinica, December, Taipei, Taiwan. 1988 2nd Midwest Tropical Diseases Meeting, University of Wisconsin, March, Madison, WI. 1988 41st Ann. Meeting, Society of the Protozoologists, July, Bristol, England. (Hutner Award Speaker). 1988 P & S Biomedical Sciences Symposium “Immune Recognition & Evasion: Molecular Aspects of Host-parasite Interaction”, June, Arden House, Harriman Campus, Columbia University, NY. 1988 2nd SCBA Int. Symposium and Workshop - Parasitology, June, Berkley, CA (Chair). 1988 Symposium of Molecular Basis of Pathogenesis in Parasitic Diseases, Dec., University of Hong Kong. 1989 Int. Symp. Molecular Parasitology, IBMS, Academia Sinica, April 24-26, Taipei, Taiwan. 1989 8th Int. Congr. Protozool., Symposium on “Intracellular surviving mechanism of protozoa”, July 19-21, Tsukuba, Japan (Chairman) (Declined). 1990 AAAS Seminar “Biology of Parasitism”, Feb. 16-18, New Orleans. 1990 3rd SCBA Int. Symposium and Workshop - Infectious Diseases, June 24-30, Hong Kong. 1990 International Symposium of Medical Parasitology, November 13-16, Shanghai, PR China. 1991 Ann. Meeting Society of Protozoologists, Symposium “Molecular Approaches to the Study of

Endosymbiosis in Protozoa”, June, Bozeman, MT. 1991 Molecular Parasitology Seminar Series, Meharry Medical College “Molecular determinants of Leishmania virulence”, April, Nashville, TN. 1992 International Symposium: Current Trend. Leishmania Research, Feb., 1992, IICB, Calcutta, India (Inauguration and Closing Keynote Speaker). 1992 CSIR Golden Jubilee Symposium, Tropical Diseases and Control Strategies, Chemotherapy & Drug

Design, Feb., Lucknow, India (Chair). 1992 3rd CEC Euroleish Meet. on Leishmaniasis, “Molecular Biology of Leishmania Virulence”, Mar., 1992, Balneario de Archen, Murcia, Spain (Guest Lecturer). 1992 4th International SCBA Symposium ”Molecular Parasitology”, June , Singapore. 1993 Symposium of Host-Parasite interactions, Joint Meet. Am. Society of Trop. Med. & Am. Society of Parasitology., Nov., Atlanta.

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INTERNATIONAL AND NATIONAL SYMPOSIA: INVITED SPEAKER/CHAIRMAN: (CON’T) 1994 XXVI Congr. Turkish Society of Microbiology, April, Antalya, Turkey. 1994 VIII International. Congress of Parasitology, October, Izmir, Turkey (Round Table Chair). 1995 National Congress of Turkish Society of Parasitology, May, Antalya, Turkey. 1995 Ist International Congress of Fever/Environ., October, Cairo, Egypt 1995 IV Seminars of Pan Arab Zoonotic Diseases, November, Amman, Jordan 1996 Global Meet in Parasitic Diseases, March, New Delhi, India (Symposium Chair) 1996 International Symposium on leishmaniasis, Baranas Hindu Univ., March, Varanasi, India 1996 XIV International Congress of Tropical Medicine & Malaria, November, Nagasaki, Japan 1997 WorldLeish I, May, Istanbul, Turkey (Organizer, Symposium Chairman, speakers) 1998 Endocytobiology VII - International Congress of Symbiosis & Biomedicine, April, Freiburg, Germany 1998 International Congress of Parasitology IX, Makihari, Japan (Session Chairs) 2000 Millennium Meeting on Porphyrins and Porphyrias 2000, Institut Pasteur, Paris, France 2002 WorldLeish II, Hersonissos, Crete, Greece (Chair, Scientific Program Committee) 2002 “Int. Conf. Physiol., Immunol. and Aspects of Cancer”, Hanoi Cancer Soc & US-Viet Found, Hanoi 2002 “Advances in Biosciences”, Tokyo University, Tokyo 2003 “Awaji International Forum for Infection and Immunity, Osaka, Japan 2004 “Chicago Area Molecular Parasitology/Mycology” special lecture, Urbana, IL 2004 “1st National Workshop on leishmaniasis” special lecture, Adana, Turkey 2004 “Plenary Forum”, WorldLeish III, Sicily, Italy 2005 “Chemotherapy & Drug Resistance Plenary Session”, XII Int. Cong. Protozool, Kuangzhou, PR China 2005 “Photodynamic chemotherapy and immunotherapy”, 4th Med. Conf. Pharmacy, Mersin, Turkey 2006 Leishmaniac jihad”, Int Conf Env Parasit/Commun Heal Initiativ., Aug Found Dayalbagh Educational

Institute, October 13 - 15, 2007Agra, India. 2007 “Ambiquity of multiple DNA versus single-copy protein coding genes for Leishmania genotyping”.

“WorldLeish 4”, Feb. 3-9, Lucknow, India.

2008 “Photodynamic vaccination with porphyric suicidal mutants against leishmaniasis”, “WorldLeish 4”, Feb. 3-9, Lucknow, India.

22


INTERNATIONAL AND NATIONAL SYMPOSIA: INVITED SPEAKER/CHAIRMAN: (CON’T) 2009 “Leishmania releases nucleoside diphosphate kinase to prevent apoptosis of macrophages”.

“WorldLeish 4”, Feb. 3-9, Lucknow, India. 2009 “Immunity to Leishmaniasis and Vaccination”, International Symposium on Leishmaniasis Vaccines,

March 9-10, Olinda Convention Center, Recife, Brazil. 2009 “Elucidation of paraisitic mechanisms from laboratory to clinico-epidemiologically relevant

Leishmania for developing effective prophylactic and therapeutic strategies”, US-NIH-NIAID/Inst Pasteur Tunis Joint Conference “Leishmania: Collaborative Research Opportunities in North Africa and the Middle East” June 22-25, Tunis, Tunisia.

2009 “Leishmania mechanisms of parasitism: Strains/species of clinico-epidemiological relevance for

leishmanization without leishmaniasis ?”, XIV Colombia Cong Parasitol/Trop Med, Medillin, Colombia, Oct. 8-11.

2010 “Development of photomedicines to minimize drug-resistance”, Symposium #95 “Current challenges in

managing visceral leishmaniasis in Indian subcontinent”, Am Soc Trop Med Hyg Ann Meet, Nov. 5, Atlanta, GA

2011 “40 years of leishmaniac research: Dennis makes it all possible !”, Cell Biology & Immunology of

leishmaniasis Symposium, Laboratory of Parasitic Diseases, NIAID, NIH, September 30, 201, Bethesda, MD

2012 “Photodynamically inactivated Leishmania as a potential novel carrier for vaccine delivery against

infectious and non-infectious diseases”, Turkish Soc. of Infectious Diseases and Clinical Microbiology, May 10th, Green Park, Istanbul, Turkey. 2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine

carriers against infectious and non-infectious diseases”, Proc. 2nd Int. Conf. Vaccines/vaccination, Aug. 20, Northbrook, IL, USA

2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine

carriers against infectious and non-infectious diseases”, 2nd Int. Symp. Leishmaniasis vaccines, September 4th Ouro Preto, Brazil.

2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine

carriers against infectious and non-infectious diseases”, Int. Conf. Clinc. Cell Immunology, Oct. 24, Hilton Chicago-North Shore, IL, USA

2013 “Photo-inactivation of Leishmaia with ROS for vaccination against leishmaniass and other infectious

diseases, Nat. Symp. Of Inf. Dis. June 10-14, Sanliurfa, Turkey. 2014 “Vector/vectorpborne diseases and molecular parasitology/tropical medicine” International Mini-

Symposium, September 10, Department of Entomology, National Taiwan University. 2014 “Photodynamic vaccination: Novel strategy of vaccine delivery by intraphagolysosomal parasites, and

their oxidative inactivation for safe and effective applications” 2nd Int. Congr. Bacteriol/Inf. Dis., November 17-19, Chicago, USA.

2015 “Visceral Leishmaniasis Control and Elimination: Is there a Clear Role for Vaccines in Achieving

Regional and Global Goals” NIH/NIAID, workshop Sept. 9-10, 2015 in Rockville, MD, USA.

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1015 Novel strategy for safe and effective vaccine delivery to elicit cell-mediated immunity against infectious and malignant diseases. December 2, Vaccine R&D-2015, San Francisco, CA, USA.

2016 “New ‘light’ for One-World approach toward safe and effective controls of animal diseases and insect

vectors”, 11th CVBD World Forum, May 9-12, Miami, FL. 2016 “One-Health perspectives of leishmaniasis in South and East Asia”, 11th CVBD World Forum, May 9-

12, Miami, FL. 2017 “Tarim hare s reservoir of desert-type visceral leishmaniasis in Xinjiang, China ? WL6, May 16-20,

Toledo, Spain. 2018 “Progress toward development of photodynamic vaccination against infectious/malignant disease and

photodynamic insecticides, SPIE WEST BIO, Jan. 31, San Francisco, CA.

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PARTICIPANT AS HOST LABORATORY FOR FOREIGN SCHOLAR VISIT OR TRAINING: 1980 NIH-PAHO International Education Branch OIA/HRA (Dr. A. Blanco, Professor of Biochemistry, Argentina). 1981 Rockefeller Foundation International Health Program (Dr. H.L. Zhong, Professor and Director of Beijing Tropical Medicine Research Institute). 1984 WHO Special Program for Research & Training in Tropical Diseases (Dr. You-yun Yi, Professor of Parasitology, First Medical College, Beijing). 1984 NSF U.S.-India Cooperation Science Programme (Dr. D.K. Ghosh, Director Immunochemistry, Calcutta School of Tropical Medicine). 1988 UNDP Project: Molecular Biology/Biotechnology of Parasites (Project Coordinator, Dr. A.N. Bhaduri, Indian Institute of Chemical Biology, Calcutta, India). 1989 UNDP Project: Leishmaniasis (Dr. M.K. Basu, Head, Biomembrane Division, Indian Institute of Chemical Biology, Calcutta, India). 1991 Turkish Ministry of Health: Leishmaniasis in Turkey (Dr. Hasan Aksu, Assoc. Prof., Clinical Microbiology, Cukurova Univ. Med. Sch., Adana, Turkey) 1992 USAID Research Program: Mechanism of drug-resistance in Peruvian clinical Leishmaniasis (Helmer Lazama, Inst. Trop. Med. “Alexander von Humboldt”, Lima, Peru) l993 Fulbright Scholarship: Leishmania and leishmaniasis in Balochistan (Dr. M. Masoom Yasinzai, Institute of Biochemistry, University of Baluchistan, Pakistan) l993 USAID Research Program:Mechanism of drug-resistance in Peruvian clinical Leishmaniasis (Dr. Jorge Arevalo, Inst. Trop. Med. “Alexander von Humboldt, Lima, Peru) l993 NIH/NIAID: Leishmania & leishmaniasis in PR China (Dr. Jing-Qi Qu, Shanghai Inst. Parasit. Dis., Shanghai, PR China) 1994 DBT/India: Drug-resistance of Indian leishmaniasis (Dr. Sarman Singh, All Indian Institute of Medical

Sciences, New Delhi, India) 1996 US/Vietnam Foundation: Anti-microbial/Anti-tumor herbal compounds indigenous to Vietnam (Dr. Dinh Tao Nguyen, Nat. Inst. Burn/Cancer Res. Center, Hanoi, Vietnam) 1996 NATO Scientific Affairs: Sero-diagnosis and -epidemiology of leishmaniasis in Turkey (Seray Osenzoy, Department of Parasitology, Ege University, Izmir, Turkey) 1996 All India Institutes of Medical Sciences: Expression of Plamodium vivax genes in Leishmania (Mohamad Fakruddin, New Delhi, India) 1997 Egypt Educational Bureau Training Fellowship: Diagnosis of parasitic diseases by ELISA and PCR (Dr. E. A. N. El-Sherif, Parasitology, Ain Shams University, Cairo) 1998 WHO Research Fellowship: Expression of Plasmodium TopoII genes in Leishmania. (Dr. Suttiphan

Kitcaroen, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand)

25


PARTICIPANT AS HOST LABORATORY FOR FOREIGN SCHOLAR VISIT OR TRAINING: 1999 TDR/WHO/UNDP: A simian model of visceral leishmaniasis for studying immunology of vaccination

(Dr. A. Dube, CDRI/S. Naik, Sanja Gandi Postgraduate Institute Medical Science, Lucknow, India) 2000 DBT/India: Episomal expression of kDNA minicircle genes for import into mitochondria (Dr. Neeloo

Singh, CDRI, Lucknow, India) 2001 TDR/WHO Research Fellowship: Transfection of Leishmania panamensis with metocyclogenesis-

specific META-1 (Dr. Carlos Muskus, Univ. Antioquia, Medellin, Colombia). 2006 Indian Institute of Immunology: Leishmania receptor for hemoglobin (Dr. Amitabha Mukhopadhyay, New Delhi, India). 2007 Training fellowship for DNA sequencing diagnostics (Dr. Seda Tezcan, Mersin University College of

Medicine, Mersin, Turkey. 2008 Associate Fellowship, Indian Department of Biotechnology (Dr. Tanmoy Bera, Pharmaceutical

Technology, Medicinal Biochemistry, Jadavpur University, Calcutta, India. (Pending) 2009 Student Research Internship, Fatih University, Istanbul, Turkey (Fatma Kurtoglu: Genetic engineering

Sophomore). 2010 PhD student Trainee, Dept Microbiology/Immunology, Faculty of Medicine, University of Mersin,

Mersin Turkey (Esin Ayden: Clinical immunology of cutaneous leishmaniasis). 2011 Exchange Scholar, Central Drug Research Institute, Lucknow, India (Neeloo Singh, Senior Investigator

for project development). 2012 Exchange Scholar, Department of Dermatology, National Institute of Health, Bogota, Colombia

(Clemencia Ovalle, Investigator/PhD candidate on Leishmania-host molecular interactions) 2013 Visiting Fellow, Tropical Disease Research Center, Cukurova University School of Medicine, Adana,

Turkey (Meral Miraloglu, Assistant Professor of Clinical Microbiology). 2016-18 Visiting DVM collaborators, Univ. Naples Federico II/RFUMS Research Collaboration Agreement,

(Laura Mann/Raffaele Corso, Attending DVM, Small Animl Clinic, Univ. Naples School of Veterinary Medicine and Animal production, Naples, Italy)

26

KPChang CV (Con’t) INVITED PRESENTATIONS AT INSTITUTIONS: 1972 University of Toronto School of Medicine, Dept. Pathology, Canada 1972 Rockefeller University, Parasitology, New York, NY 1976 New York University, Department of Microbiology, New York, NY 1977 Columbia University School of Public Health, Division of Tropical Medicine, New York, NY 1978 Cornell University School of Medicine, Division of International Health, New York, NY 1978 New York University, Department of Preventive Medicine, New York, NY 1979 Trudeau Institute, Saranac Lake, NY 1979 Beijing Tropical Medicine Research Institute, PR China 1979 Imperial College University of London, U.K. 1980 Case Western Reserve University, Dept. Geographic Medicine, Cleveland, OH 1980 Merck Institute of Research, Rahway, NJ 1980 International Institute of Cell and Molecular Pathology, Brussels, Belgium 1980 Pasteur Institut, Paris, France 1981 New York Institute of Public Health, New York, NY 1981 University of California San Francisco, Department of Pharmacology, San Francisco, CA 1981 Zhongshan University, Department of Biology, Guangzhou, China 1981 Tokyo University School of Medicine, Third Department of Medicine, Tokyo, Japan 1982 Louisiana State University, Department of Biology, Baton Rouge, LA 1982 State University of New York, Department of Microbiology, Syracuse, NY 1982 Indiana State University, Department of Biology, Terre Haute, IN 1982 UHS/Chicago Medical School, Department of Microbiology, Chicago, IL 1984 Beijing Tropical Medicine Research Institute, Friendship Hospital, Beijing 1984 Shandong Institute of Parasitic Diseases, Jinin, Shandon, China 1984 Shanghai Institute of Parasitic Diseases, Shanghai, China 1984 Sichuan Medical College, Chengdu, Sichuan, China 1984 Ionian University School of Medicine, Ionian, Greece 1984 Pasteur Institut Hellenique, Athens, Greece 1985 UHS/Chicago Medical School, Department of Pharmacology, North Chicago, IL 1986 UHS/Chicago Medical School, Department of Physiology, North Chicago, IL 1986 University of Rochester, Department of Microbiology, Rochester, NY 1986 Medical College of Wisconsin, Department of Biochemistry, Milwaukee, WI 1986 University of Illinois, Department of Biochemistry, Chicago, IL 1987 University of Granada, Department of Parasitology, Granada, Spain 1987 National Taiwan University School of Medicine, Department of Parasitology, Taipei, Taiwan 1988 Abbott Diagnostic Division, North Chicago, IL 1988 Peking University, Department of Biology, Beijing, China 1988 Shandon Institute of Parasitic Diseases, Jinin, Shandon, China 1988 Municipal Department of. Public Health, Dalian, China 1988 Institute of Biological Products, Changchun, Jilin, China 1988 West China University of Medical Science, Chengdu, Sichuan, China 1988 Institute of Parasitic Diseases, Center of Preventive Medicine, Shanghai, China 1988 University of California, Department of Pharmaceutical Chemistry, San Francisco, CA 1988 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan 1989 North Carolina State University, School of Public Health, Chapel Hill, NC

27


INVITED PRESENTATIONS AT INSTITUTIONS: (CON’T) 1990 University of Iowa, Dept. Biology, Iowa City, IA 1990 Xiamen University, Parasitology Research Laboratory, Fujian, China 1991 University of Illinois, School of Veterinary Science, Department of Pathobiology, Urbana, IL 1991 UHS/Chicago Medical School, Molecular & Cellular Sciences Retreat, Zion, IL 1992 Tokyo University, Faculty Agriculture, Tokyo, Japan 1992 University of Nagasaki School of Medicine, Nagasaki, Japan 1992 National Institute of Endemic Diseases, Urumqi, Xinjing, China 1992 UHS/Chicago Medical School, Department of Biochemistry, North Chicago, IL 1993 Purdue University, Veterinary Pathobiology, West Lafayette, IN 1993 University of Georgia, Department of Biology, Athens, GA 1994 Ege University Faculty of Medicine, Department of Parasitology, Izmir, Turkey 1994 Cukurova University/Medical Faculty, Department of Clinical Bacteriology, Adana, Turkey 1994 Municipal Department of Health, Urfa, Turkey 1994 Laboratory of Protozoology, National Institute of Animal Health, Tsukuba, Japan 1994 Seminars in Clinical Research Series, Rockefeller University, NY 1995 Shihezi School of Medicine, Shihezi, Xinjiang, China 1995 Laboratory of Bacteriology, National Institute of Animal Health, Tsukuba, Japan 1995 NAMRU-II, Cairo, Egypt 1995 Ains Sham University, Cairo, Egypt 1996 All India Institute of Medical Sciences, Dept. Laboratory Medicine, New Delhi, India 1996 All India Institute of Medical Sciences, Department of Internal Medicine, New Delhi, India 1996 National Institute of Immunology, New Delhi, India 1996 University of Delhi S. Campus, Dept. Biochemistry, Genetics, Microbiology, New Delhi, India 1997 J. Nehru University, Life Sciences, New Delhi, India 1997 Indian Institute of Chemical Biology, Calcutta, India 1997 TK Jha Kala-azar Research Center, Muzaffarpur, Bihar, India 1997 Indian Society of Parasitology, Central Drug Research Institute, Lucknow, India 1997 Dept. Experimental Medicine/Parasitology, Postgrad. Instistute of Med. Edu. Res., Chandigarh, India 1998 Institute of Genetics, Fudan University, Shanghai, PR China 1998 Department of Parasitology, Ege University, Izmir, Turkey 1998 Institute of Preventive Medicine, Baku, Azerbaijan 1998 Institute of Epidemiology, Ministry of Health & Academy of Natur. Sci, Ashkabat, Turkmenistan 1998 Institute of Medical Epidemiology, Ministry of Health, Tashkent, Uzbekistan 1998 Isaev Institute of Medical Parasitology, Samarkan, Uzbekistan 1998 Infectious Disease Section, Ministry of Health, Bishkek, Gyrgys Republic 1998 Monbusho Annual Meeting, Makahari, Tokyo, Japan 1999 Department of Microbiology/Immunology, University of Illinois at Chicago Circle 1999 Chicago Area Molecular Parasitology Group, Northwestern University, Chicago 1999 Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan 1999 Institute of Preventive Medicine, Defense Department School of Medicine, Taipei, Taiwan 1999 Department of Dermatology, Damascus University, Damascus, Syria 2000 Department of Parasitology, Charles University, Prague, Czech Republic 2000 Developmental Cell Biology Program, Rutgers University, Piscataway, NJ 2001 Municipal Center for Disease Control, Guiyang, Guizhou, PR China 2001 Department of Parasitology, Western China Medical University, Chengdu, Sichuan, China 2002 Department of Biochemistry and Molecular Biology, FHUS/Chicago Med School, N Chicago, IL 2002 Cathy Hospital Research Institute, Siji, Taipei, Taiwan 2002 Molecular Biotechnology, Taipei Medical University, Taipei, Taiwan 2002 Department of Parasitology, National Taiwan University School of Medicine, Taipei, Taiwan 2002 Cathy Hospital, Taipei, Taiwan 2002 Institute of Biochemistry, Cheng Kung National University, Tainan, Taiwan

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INVITED PRESENTATIONS AT INSTITUTIONS: (CON’T) 2002 Department of Parasitology, Cheng Kung National University, Tainan, Taiwan 2002 Department of Pathology, University of Texas, Medical Branch, Galveston, TX 2002 Department of Biotechnology, Shanghai Institute of Biological Products, Shanghai, PR China. 2002 Department of Biotechnology, Hong Kong Polytech University, HK. 2002 Department of Parasitology, National Taiwan University College of Medicine, Taipei, Taiwan. 2003 Department of Entomology, National Taiwan University, Taipei, Taiwan. 2003 School of Agricultural Sciences, Chun Hing University, Taichung, Taiwan. 2003 School of Biotechnology, Xinjiang University, Urumqi, Xinjiang, PR China. 2003 Department of Molecular Immunology, Faculty of Agricultural Sciences, University of Tokyo, Japan. 2004 Medical Center, Ain Sham University, Cairo, Egypt. 2004 Department of Internal Medicine & Infectious Diseases, Cukurova University, Adana, Turkey. 2005 Photomedicine Lecture Series, Wellman Center, Massachusetts General Hospital, Boston, MA. 2005 Departments of Microbiology, Plant Pathology, Entomology, Nat Taiwan University, Taipei, Taiwan. 2005 Department of Biology, Tong-Hai University, Taichung, Taiwan. 2005 Department of Biology, Chang-Hua Normal University, Chang-Hua, Taiwan. 2005 Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. 2005 Department of Biotechnology, National Taipei University of Technology, Taipei, Taiwan. 2005 Institute of Biological Products, Shanghai, PR China. 2005 Faculty of Medicine, Mustafa Kemal University/Municipal Ministry of Health, Antakya, Turkey. 2006 CAMP (Chicago Area Molecular Parasitology), Northwestern University, Chicago. 2006 School of Basic Science, Tongji University, Shanghai, China. 2006 Department of Chemistry, Fudan University, Shanghai, China. 2006 Department of Etiologic Biology, 2nd Military Medical University, Shanghai, China. 2006 Infectious Disease Division, Cukurova University School of Medicine, Adana, Turkey. 2006 Institute of Parasitology, Academy of Sciences, Ceske Budejovice, Czech Republic. 2007 Department of Microbiology/Immunology, University of Illinois, Chicago, IL 2007 “Porphyric Leishmania as suicidal mutants for vaccination”, Yildiz University, Istanbul, Turkey. 2007 “Porphyric Leishmania as suicidal mutants for vaccination”, IICB, Kolkata, India. 2007 “Porphyric Leishmania as suicidal mutants for vaccination”, CDRI, Lucknow, India. 2007 “Porphyric Leishmania as suicidal mutants for vaccination”, AIIMS, New Delhi, India. 2007 “Porphyric Leishmania as suicidal mutants for vaccination”, NII, New Delhi, India. 2008 “Leishmania infection and virulent phenotypes, NCSS, Pune, India. 2008 “Suicidal porphyric Leishmania for photodynamic vaccination”, Parasitology, Pune Univ., India. 2008 “Leishmania nagt genotypes and leishmaniasis”, PGIMS, Chandigarh, India. 2008 “Toward suicidal automation of porphyric Leishmania for photodynamic vaccination”, Materia

Medica, Peking Union Medical Institute, Beijing, PR China. 2008 “Toward suicidal automation of porphyric Leishmania for photodynamic vaccination”, Capital Medical

University, Beijing, PR China. 2008 “Toward suicidal automation of porphyric Leishmania for photodynamic vaccination”, Central for

Disease Control, Urumqi, Xinjiang, PR China. 2008 “Toward suicidal automation of porphyric Leishmania for photodynamic vaccination”, Institute of

Biotechonology, Xijiang University, Urumqi, Xinjiang, PR China. 2008 “Toward suicidal automation of porphyric Leishmania for photodynamic vaccination”, Institute of

Biological Sciences, Zhongshan University, Guangzhou, PR China. 2009 “Leishmania mechanisms of parasitism exploitable for photodynamic prophylaxis and

therapy“,Bioinformatics of Intracellular Pathogen- workshop 7-9th Feb. 2009, JUN Campus, N Delhi, India.

2010 “From endosymbiosis to leishmanic jihad”, Dept. Biol Science, Chicago State Univ, Chicago, IL 2010 “Leishmaniac jihad: Let the evils fight the devils”, Dept. Chemistry, Illinois State Univ, Normal, IL 2011 “Leishmania model for molecular epidemiology/microbial virulence of vector-borne diseases” Institute of Biotechnology, National University of Education, Dec. 20Changhwa, Taiwan

29


INVITED PRESENTATIONS AT INSTITUTIONS: (CON’T) 2011 “From intracellular symbiosis/parasitism to the development of photodynamic medicine/insecticide”

Institute of Biotechnology, National University of Education, Dec. 26, Changhwa, Taiwan 2011 “~40 years of personal experience: Biological and biomedical research/Funding and career path”

Institute of Biotechnology, National University of Education, Dec. 27, Changhwa, Taiwan 2011 “From intracellular symbiosis research to the development of photomedicine”, Dec, 28 Dept of

Entomology, National Chung Hsing University, Taichung, Taiwan 2012 “Leishmania and leishmaniasis as a zoonotic disease”, Department of Microbiology/Immunology, Jinan

University School of Medicine, Mar. 5, Guangzhou, China 2012 “Leishmania model for microbial virulence in infectious diseases”, Department of

Microbiology/Immunology, Jinan University School of Medicine, Mar. 8, Guangzhou, China 2012 “Photo-inactivated eishmania carrier for vaccination against infectious/non-infectious diseases”,

Department of Microbiology/Immunology, Jinan University School of Medicine, Mar. 12, Guangzhou, China

2012 “Photodynamic pesticides”, Department of Microbiology/Immunology, Jinan University School of Medicine, Mar. 15, Guangzhou, China

2012 “Photo-inactivated Leishmania carrier for vaccination against infectious/non-infectious diseases”, School of Public Health/Tropical Medicine, Southern Medical University, Mar. 16, Guanzhou, China

2012 “Photodynamic therapy of leishmaniasis and other diseases”, Department of Microbiology/Immunology, Jinan University School of Medicine, Mar. 19, Guangzhou, China

2012 ~40 years of personal experience in biological/biomedical research, Department of Microbiology/Immunology, Jinan University School of Medicine, Mar. 22, Guangzhou, China

2012 “Leishmania and leishmaniasis”, Department of Biology, Teacher College, King Saud University, May 5, Riyadh, Saudi Arabia

2012 “Host-parasite cellular and molecular interactions in Leishmania infection of macrophages”, King Faisal Specialists Hospital/Research Center, Riyadh, May 5, Saudi Arabia

2012 “Leishmania model for Microbial virulence”, Department of Zoology, King Saud University, Riyadh, May 6, Saudi Arabia

2012 “From intracellular symbiosis research to the development of photomedicine”, Department of Bioengineering, Yildiz Technical University, May 8, Istanbul, Turkey

2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine carriers against infectious and non-infectious diseases”, National Taiwan University School of Medicine, Sep. 26, Taipei, Taiwan.

2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine carriers against infectious and non-infectious diseases”, Chang Gang University School of Medicine, Sep. 28, Taipei, Taiwan

2012 “Integration of Agi- and Medi-pest insect control: One-health initiatives, National Taiwan University, Department of Entomology, Oct. 2, Taipei, Taiwan.

2012 “Photo-inactivation of Leishmania with ROS via chemical and genetic approaches as novel vaccine carriers against infectious and non-infectious diseases”, Academia Sinica, Oct. 8th, Taipei, Taiwan

2013 “Photo-inactivation of Leishmania with ROS for safe and effective delivery of vaccines against infectious and malignant diseases, March 20, National Institute of Parasitic Diseases, Shanghai, China.

2013 “Photo-inactivation of Leishmania with ROS for safe and effective delivery of vaccines against infectious and malignant diseases, March 21, Public Health Clinical Center-Fudan University, Shanghai, China.

2013 “Photo-inactivation of Leishmania with ROS as vaccines/vaccine carriers against infectious and malignant diseases”, June12, National Symposium of Infectious Diseases, Sanliurfa, Turkey.

2013 “Photo-inactivation of Leishmania with ROS as vaccines/vaccine carriers against infectious and malignant diseases”, Aug. 29, “Awareness Week of Leishmaniasis”, FIOCRUZ, Rio, Brazil

2013 “Leishmaniasis and vaccination”, October 17, School of Veterinarian Medicine, University of Napoli, Italy.

2013 “Photo-inactivation of Leishmania with ROS as vaccines/vaccine carriers against infectious and malignant diseases, Nov. 20, National Taiwan University School of Medicine, Taipei, Taiwan.

30


INVITED PRESENTATIONS AT INSTITUTIONS: (CON’T) 2013 “Leishmania model for microbial virulence and vaccination of zoonotic diseases”, Nov. 22, National Taiwan

University School of Veterinarian Medicine, Taipei, Taiwan. 2013 “Leishmaina model for molecular epidemiology and vaccination of zoonotic diseases”, Nov. 26, National Taiwan

University Department of Entomology, Taipei, Taiwan 2013 “Development of photo-vaccines against infectious and malignant diseases”, Nov. 29, Institute of International

Health and Tropical Medicine, Taipei Medical University, Taipei, Taiwan. 2014 “Photodynamic vaccination: Novel strategies of vaccine delivery by intraphagolysosomal parasites and their

oxidative inactivation for safe and effective applications, May 15th, Department of Microbiology/Immunology, Meharry Medical School, Nashville, TN.

2014 “Leishmania photodynamic vaccination”, September 5, Department of Molecular Immunology, University of Tokyo.

2014 “Vaccination for disease prevention and control: The necessity of renewed emphasis and new approaches”, October 9th, Johnny Outzs Memorial Lectures, Delta State University, W. Cleveland, MS.

2015 “Oxidative photo-inactivated Leishmania for safe and effective vaccination”, Feb. 16, Department of Microbiology/Immunology, University of Illinois at Chicago, College of Medicine, Chicago, IL.

2015 “"New strategies to control disease-transmitting insect vectors: Photodynamic and transgenetic approaches", National Taiwan University, College of Biological Resources/Agriculture, Entomology, Dec 14, Taipei, Taiwan.

2015 “Novel strategy of cell-mediated immunotherapy for treatment of malignant diseases”, Chang Gang University School of Medicine, Dec. 18, Taoyuang, Taiwan.

2015 “Porphyrins and phthalocyanines: New strategies for applications in biology and medicine”, Academia Sinica Institute of Chemistry, Dec. 29, Nankang, Taiwan.

2016 "Photodynamic and transgenetic strategies to control disease-transmitting insect vectors and other harmful pests", National Taiwan University, College of Biological Resources/Agriculture, Entomology, Dec 14, 2016, Taipei, Taiwan. (Host: Dr. Wen-Lung Wu).

2016 “Leishmania-mediated immunotherapy against malignant diseases”, Taipei Medical University, Dec. 20, 2016, Taipei, Taiwan. (Host: Dr. Chia-Kwung Fan).

2016 “Ovalbumin as a surrogate vaccine against LSTRA murine tumor model”, Chang Gang Univ, Tao Young, Taiwan Jan. 3, 2017 (Host: Dr. Chao-Lan Yu).

2016 “Light-activable mosquito larvicides”, Department of Parasitology School of Medicine, Jan, 5, 2017, Taipei, Taiwan. (Host: Dr. Shiao Shin-Hong).

2017 “Visceral leishmaniasis special lecture and discussion session”, Xinjiang CDC, June 21, 2017, Urumqi, Xijinag, PR China.

2017 “Photodynamic vaccination and photodynamic insecticide development”, December 28, Academia Sinica, Nankan, Taipei, Taiwan.

2018 “Photodynamic vaccination for immunotherapy of malignant diseases”, January 8th, Division of experimental and clinical Tumor Research, National Health Research Institute, Tainan, Taiwan.

31

KPChang CV (Con’t) PUBLICATIONS: A total of ~130 peer-reviewed papers by Pubmed-search for “chang kp, leishmania (103); “chang kp,symbiotes (10) or symbionts (2) or endosymbionts (3) or endosymbiosis (9)” Journal Articles:

1. Chang, K.-P. & Musgrave, A.J. (1969) Histochemistry and ultrastructure of the mycetome and its “symbiotes” in the pear psylla, Psylla Pyricola Foerster (Homoptera). Tissue and Cell 1: 597-606.

2. Chang, K.-P. & Musgrave, A.J. (1970) Ultrastructure of rickettsia-like microorganisms in the midgut of a

plant bug, Stenotus binotatus Jak. (Heteroptera: Miridae). Can. J. Microbiol. 16: 621-622. 3. Chang, K.-P. & Musgrave, A.J. (1972) Multiple Symbiosis in a leafhopper, Helochara communis fitch

(Cicadellidae: Homoptera): envelopes, nucleoids and inclusions of the symbiotes. J. Cell Sci. 11: 275-293. 4. Chang, K.-P. & Musgrave, A.J. (1973) Morphology, histochemistry and ultrastructure of mycetome and its

rickettsial symbiotes in Cimex lectularius (L.) Can. J. Microbiol. 19: 1075-1081. 5. Chang, K.-P. (1974) Effects of elevated temperature on mycetome and its symbiotes of Cimex lectularius

(L.) J. Invertebr. Pathol. 23: 333-340. 6. Chang, K.-P. & Trager, W. (1974) Nutritional significance of symbiotic bacteria in two species of

hemoflagellates. Science 183: 531-532. 7. Chang, K.-P. (1974) Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia

culicis and Crithidia oncopelti. J. Protozool. 21: 699-707. 8. Chang, K.-P. & Musgrave, A.J. (1975) Conversion of spheroplast symbiotes in a leafhopper, Helochara

communis Fitch (Cicadellidae: Homoptera). Can. J. Microbiol. 21: 196-204. 9. Chang, K.-P. & Musgrave, A.J. (1975) Endosymbiosis in a leafhopper Helochara communis Fitch

(Cicadellidae: Homoptera): symbiote translocation and auxillary cells in the mycetome. Can J. Microbiol. 21: 186-195.

10. Tuan, R.S. & Chang, K.-P. (1975) Isolation of intracellular symbiotes from flagellate protozoa by immune

lysis and characterization of their DNA. J. Cell Biol. 65: 309-323. 11. Chang, K.-P., Chang, C.S. & Sassa, S. (1975) Heme biosynthesis in bacterium-protozoan symbioses:

enzymic defects in host hemoflagellates and complemental role of their intracellular symbiotes. Proc. Nat. Acad. Sci. USA 72: 2979-2983.

12. Chang, K.-P. (1975) Reduced growth of Blastocrithidia culicis and Crithidia oncopelti freed of intracellular

symbiotes by chloramphenicol. J. Protozool. 22: 271-276. 13. Dwyer, D.M. & Chang, K.-P. (1976) Surface membrane carbohydrate alteration of a flagellate protozoan

mediated by bacterial endosymbionts. Proc. Nat. Acad. Sci. USA 73: 852-856. 14. Chang, K.-P. (1976) Symbiont-free hemoflagellates (Blastocrithidia culicis and Crithidia oncopelti): Their

liver factor requirement and their serological identity with infected strains. J. Protozool. 23: 241-244. 15. Chang, K.-P. & Dwyer, D.M. (1976) Multiplication of a human parasite (Leishmania donovani) in

phagolysosomes of hamster macrophages in vitro. Science 193: 678-680.

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PUBLICATIONS: Journal Articles: (con’t) 16. Meshnick, S.R., Chang, K.-P., & Cerami, A. (1977) Heme lysis of the bloodstream forms of Trypanosoma

brucei. Biochem. Pharmacol. 26: 1923-1928. 17. Chang, K.-P., Steiger, R.F., Dave, C. & Cheng, Y.-C. (1978) Effects of methylglyoxal bis

(guanylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in Trypanosoma brucei. J. Protozool. 25: 145-149.

18. Chang, K.-P. & Dwyer, D.M. (1978) Leishmania donovani-hamster macrophage interactions in vitro: cell

entry, intracellular survival and multiplication of amastigotes. J. Exp. Med. 147: 515-530. 19. Chang, K.-P. (1978) Hamster peritoneal macrophages in vitro: substratum adhesion, spreading,

phagocytosis and phagolysosome formation. In vitro 14: 663-674. 20. Chang, K.-P. (1978) Intracellular multiplication of Leishmania donovani during repeated passages in

primary cultures of hamster peritoneal macrophages. J. Parasitol. 64: 931-933. 21. Chang, K.-P. (1978) Leishmania infection of human skin fibroblasts in vitro: absence of phagolysosomal

fusion after induced phagocytosis of promastigotes and their intracellular transformation. Am. J. Trop. Med. Hyg. 27: 1084-96.

22. Chang, K.-P. (1979) Leishmania donovani: Promastigote-macrophage surface interactions in vitro. Exp.

Parasitol. 48: 175-189. 23. Chang, K.-P. (1980) Human cutaneous leishmania in a macrophage line: Propagation and isolation of

intracellular parasite. Science 209: 1240-2. 24. Chang, K.-P. (1980) endocytosis of FITC dextran by leishmania-infected macrophages, fluorimetry of

pinocytosis, lysosome-phagosome fusion and intralysosomal pH, In Host-Invader Interplays, Ed. van den Bossche, North Holland, 231-4.

25. Chang, K.-P. (1981) Leishmanicidal mechanisms of human polymorphonuclear phagocytes. Am. J. Trop.

Med. Hyg. 30: 322-33. 26. Chang, K.-P. (1981) Antibody-mediated inhibition of phagocytosis in Leishmania donovani-human

phagocyte interactions in vitro. Am. J. Trop. Med. Hyg. 30: 334-39. 27. Chang, K.-P. (1981) Leishmania donovani-hamster macrophage binding mediated by their surface

glycoproteins/antigens: Characterization in vitro by a radioactive assay. Mol. Biochem. Parasitol. 4: 67-76. 28. Carson, D.A. & Chang, K.-P. (1981) Phosphorylation and anti-leishmania activity of formycin B. Biochem.

Biophys. Res. Comm. 100: 1377-83. 29. Carson, D.A. & Chang, K.-P. (1981) Selective killing of leishmania-infected mouse macrophages by 6-

methylpurine 2’-deoxyriboside. Life Sci. 29: 1617-21. 30. Chang, K.-P., Bray, R.S. & Leaney, A.J. (1981). Infection of mouse macrophages in vitro by sandfly-

derived promastigotes of Leishmania mexicana amazonensis. Trans. Roy. Soc. Trop. Med. Hyg. 75: 475-6. 31. Fong, D. & Chang, K.-P. (1981) Tubulin biosynthesis in the developmental cycle of a parasitic protozoan,

Leishmania mexicana: Changes during differentiation of motile and non-motile stages. Proc. Nat. Acad. Sci. USA 78: 7624-8.

33


PUBLICATIONS Journal Articles: (con’t)

32. Chang, K.-P. & Chiao, D.W. (1981) Cellular immunity of mice to Leishmania donovani in vitro:

Lymphokine-mediated intracellular killing of parasites in macrophages. Proc. Nat. Acad. Sci. USA 78: 7083-7.

33. Chang, K.-P. & Fong, D. (1982) Antigenic changes during intracellular transformation of leishmanias in

cultured macrophages. Infect. Immun. 36: 430-431. 34. Fong, D. & Chang, K.-P. (1982) Surface antigenic change during differentiation of a parasitic protozoan,

Leishmania mexicana: Identification by monoclonal antibodies. Proc. Nat. Acad. Sci. USA 79: 7366-70. 35. Wallach, M., Fong, D., & Chang, K.-P. (1982) Post-transcriptional control of tubulin biosynthesis in

leishmanial differentiation. Nature 299: 650-2. 36. Hu, X.-S. & Chang, K.-P. (1984) Vaccination of mice with formalin-killed amastigotes of Leishmania

mexicana against Leishmania donovani: Muramyl dipeptide-enhanced protection. Acta Acad. Med. Sichuan 15: 91-97.

37. Fong, D., Wallach, M., Keithly, J., Melera, P.W. & Chang, K.-P. (1984) Differential expression of mRNAs

for tubulin during differentiation of a parasitic protozoan, Leishmania mexicana. Proc. Nat. Acad. Sci. USA 81: 5782-5786.

38. Kiehlbauch, J.A., Albach, R.A., Baum, L.L. & Chang, K.-P. (1985) Phagocytosis of Campylobacter jejuni

and its intracellular survival in mononuclear phagocytes. Infect.Immun. 48: 446-451. 39. Hu, X.-S. & Chang, K.-P. (1985) Immunization of BALB/c mice with cultured amastigotes of Leishmania

mexicana amazonensis: Protection by gamma-irradiated parasites. Acta Acad. Med. Sichuan 16: 1-5. 40. Chang, C.S. and Chang, K.-P. (1985) Heme acquisition and utilization by intracellular and extracellular

stages of L. m. amazonensis. Mol. Biochem. Parasitol. 16: 267-276. 41. Chang, C.S. and Chang, K.-P. (1986) Monoclonal antibody affinity purification of a leishmania membrane

glycoprotein and its inhibition of leishmania-macrophage binding. Proc. Nat. Acad. Sci. USA 83: 100-104. 42. Chang, C.S., Inserra, T.J., Kink, J.A., Fong, D., and Chang, K.-P. (1986) Expression and size heterogeneity

of a 63 kDa membrane glycoprotein during growth and transformation of Leishmania mexicana amazonensis. Mol. Biochem. Parasitol. 18: 197-210.

43. Kink, J.A. & Chang, K.-P. (1987) Tunicamycin-resistant Leishmania mexicana: Virulent phenotype

associated with increased N-acetylglucosamine-1-phosphate transferase activity and amplification of its gene. Proc. Nat. Acad. Sci. USA 84: 1253-1257.

44. Kink, J.A. & Chang, K.-P. (1987) Biological and biochemical characterization of tunicamycin-resistant

Leishmania mexicana. Infect. Immun. 55: 1692-1700. 45. Kink, J.A. & Chang, K.-P. (1988) N-glycosylation as a biochemical basis for virulence in Leishmania

mexicana amazonensis. Mol. Biochem. Parasitol. 27: 181-190.

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46. Chaudhuri, G. & Chang, K.-P. (1988) Acid protease activity of a major surface membrane glycoprotein (gp63) from Leishmania mexicana promastigotes. Mol. Biochem. Parasitol. 27: 43-52.

47. Detke, S., Chaudhuri, G., Kink, J.A., & Chang, K.-P. (1988) DNA amplification in tunicamycin-resistant

Leishmania mexicana amazonensis. Multiple copies of a single 63 Kilobase supercoiled molecule. J. Biol. Chem. 263: 3418-24.

48. Chaudhuri, G. & Chang, K.-P. (1988) A simple method for isolation of extrachromosomal circular DNA in

unicellular eukaryotes. Nucleic Acids Research 16: 2341. 49. Detke, S. & Chang, K.-P. (1989) DNA amplification in sodium arsenite-resistant Leishmania mexicana

amazonensis. Exp. Cell Res. 180: 161-170. 50. Chaudhuri, G., Chaudhuri, M., Pan, A. & Chang, K.-P. (1989) Surface acid protease (gp63) of Leishmania

mexicana. A metalloenzyme capable of protecting liposome-encapsulated proteins from phagolysosomal degradation. J. Biol. Chem. 264:7483-9.

51. Katakura, K. & Chang, K.-P. (1989) H-DNA amplification in Leishmania resistant to both arsenite and

methotrexate. Mol. Biochem. Parasitol. 34: 189-192. 52. Olafson, R.W., Thomas, J.R., Ferguson, M.A.J., Dwek, R.A., Chaudhuri, M., Chang, K.-P. & Rademacher,

T.W. (1990) Structures of the N-linked oligosaccharides of gp63, the major surfact glycoprotein, from Leishmania mexicana amazonensis. J. Biol. Chem. 256:12240-12247.

53. Katakura K., Peng, Y., Pithawalla, R., Detke, S. & Chang, K.-P. (1991) Tunicamycin-resistant variants

from five species of Leishmania contain amplified DNA in extrachromosomal circles of different sizes with a transcriptionally active homologous region. Mol. Biochem Parasitol. 44:233-244.

54. Lee, S.-Y., Lee, S.-T. & Chang, K.-P. (1992) Transkinetoplastidy - A novel phenomenon involving bulk

alteration of mitochondrion-kinetoplast DNA of a trypanosomatid protozoan. J. Protozool. 39:190-196. 55. Liu, Xuan & Chang, K.-P. (1992) Extrachromosomal genetic complementation of surface

metalloproteinase-deficient Leishmania increases its binding to macrophages. Proc. Nat. Acad. Sci. USA 89: 4991-4995.

56. Liu, Xuan & Chang, K.-P. (1992) The 63 kb circular amplicon of tunicamycin-resistant Leishmania

contains a functional N-acetylglucosamine-1-phosphate transferase gene that can be used as a dominant selectable marker in transfection. Mol. Cell. Biol. 12: 4112-4122.

57. Liu, Xuan & Chang, K.-P. (l993) N-acetylglucosamine-1-phosphate transferase gene is conserved in five

Leishmania species and overexpressed due to its amplification in their tunicamycin-resistant variants. Mol. Biochem. Parasitol. 59: 177-180.

58. Lee, S.-T., Tarn, C. & Chang, K.-P. (l993) Characterization of the switch of kinetoplast DNA minicircle

dominance during development and reversion of drug resistance in Leishmania. Mol. Biochem. Parasitol. 58: 187-204.

59. Mensa-Wilmot, K., LeBowitz, J. H., Chang, K.-P., Al-Qahtani, A., McGwire, B. S., Tucker, S. and Morris,

J. C. (l994) A Glycosylphosphatidylinositol (GPI)-negative phenotype produced in Leishmania major by GPI phospholipase C from Trypanosoma brucei: Topology of two GPI pathways. J. Cell Biol. 124: 935-947.

35



60. Lu, H. -G., Zhong, L., Guan, L. -R., Qu, J. -Q, Hu, X. -S., Chai, J. -J., Xu, Z. -B., Wang, C. -T. and Chang,

K. -P. (l994) Separation of Chinese Leishmania isolates into five genotypes by kinetoplast and chromosomal DNA heterogeneity. Am. J. Trop. Med. Hyg. 50: 763-770.

61. Qu, J. -Q., Zhong, L., Masoom-Yasinzai, M., Rab, M. A., Aksu, H. S. Z., Reed, S. G., Chang, K. -P. and

Gilman-Sachs, A. (l994) Serodiagnosis of Asian leishmaniasis with a recombinant antigen from the repetitive domain of a Leishmania kinesin. Trans. Roy. Soc. Trop. Med. Hyg 88:543-545.

62. Liu, Xuan & Chang, K. -P. (l994) Identification by extrachromosomal amplification and over-expression of

alpha-crystallin/NADPH-oxidoreductase homologue constitutively expressed in Leishmania spp. Mol. Biochem. Parasitol. 66:201-210.

63. McGwire, B.S. & Chang, K.-P. (l994) Genetic rescue of surface metalloproteinase-deficiency in

Leishmania amazonensis variants increases their infection of macrophages at the early phase. Mol. Biochem. Parasitol. 66:345-347.

64. Du, Yubin & Chang, K. -P. (l994) Phylogenetic heterogeneity of three Crithdia spp. vs. Crithidia

fasciculata . Mol. Biochem. Parasitol. 66:171-174. 65. Du, Yubin, McLaughlin, G. and Chang, K.-P. (1994) 16S ribosomal DNA sequence identities of _-

proteobacterial endosymbionts in three Crithidia species. J. Bacteriol. 176: 3081-3084.

66. Du, Y., Maslov, D.A. and Chang, K.-P. (1994) Monophyletic origin of _-division proteobacterial endosymbionts and their coevolution with insect trypanosomatid protozoa Blastocrithidia culicis and Crithidia spp. Proc. Natl. Acad. Sci. USA 91: 8437-8441.

67. Guan, L.-R., Yang, Y.-Q., Xu, Y.-X., Qu, J.-Q., Zho, X.-P., Wang, G., Lu, H.-G., Zhong, L & Chang, K.-P.

(1994) Leishmaniasis in Karamay. XIV: Identification of isolates from naturally infected Phlebotmus major wui. Chin. J. Parasitol. Parasit. Dis. 12: 257-261.

68. Huang, M., Albach, R.A., Chang, K.-P., Tripathi, R.L.and Kemp. R.G. (1995) Cloning and sequencing a

putative pyrophosphate-dependent phosphofructokinase gene from Entomoeba histolytica. Biochim. Biophys. Acta 1260: 215-217.

69. Aksoy, S., Arturk, S., Armstrong, M.Y.K., Chang, K.-P., Gottlieb, M., Ozcel, M.A., Richards, F.F. and

Western, K. (1995) The GAP project in southern Turkey: The potential for emergence of diseases. Emerg. End. Dis. 3: 45-48.

70. Singh, S., Gilman-Sachs, A., Chang, K.-P. And Reed, S.G. (1995) Diagnostic and prognostic value of K39

recombinant antigen in Indian leishmaniasis. J. Parasitol. 81: 1000-1003. 71. Singh, S., McGwire, B.S. and Chang, K.-P. (1995) Kinetoplast and nuclear DNA polymorphism of visceral

Leishmania isolates from northern India. J. Protozool. Res. 5: 27-32. 72. Brittingham, A., Morrison, C.J., McMaster, W.R., McGwire, B. S., Chang, K.-P. and Mosser, D.M. (1995)

The role of the Leishmania surface protease, gp63, in complement fixation, cell adhesion and resistance to complement-mediated lysis. J. Immunol. 155: 3102-3111.

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73. McGwire, B.S. and Chang, K.-P. (1996) Posttranslational regulation of a Leishmania HEXXH-

metalloproteinase (gp63). The effects of site-specific mutagenesis of catalytic, zinc-binding, N-glycosylation and GPI-addition sites on N-terminal end cleavage, intracellular stability and extracellular exit. J. Biol. Chem. 271: 7903-9.

74. Chakrabarty, R., Mukherjee, S., Lu, H.-G., McGwire, B.S., Chang, K.-P. & Basu, M.K. (1996) Kinetics of

entry of virulent and avirulent strains of Leishmania donovani into macrophages: A possible of virulence gene. J. Parasitol. 82: 632-635.

75. Yasinzai-Masoom, M. & Chang, K.-P. (1996) Characterization of gp63, a major surface glycoprotein on Leishmania isolates of Pakistan. J. Parasit. Dis. 20: 11-16. 76. Kawazu, S.-I., Lu, H.G. & Chang, K.-P. (1997) Stage-independent splicing of transcripts from two heterlogous neighboring genes in Leishmania amazonensis. Gene 196: 49-59. 77. Lu, H.G., Li, Z., Chang, K.-P. & Docampo, R. (1997) Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonensis amastigotes. J. Biol. Chem. 272: 9464-9473. 78. Ozensoy, S., Ozbel, Y., Turgay, N., Alkan, M.Z., Gul, K., Gilman-Sachs, A., Chang, K.-P., Reed. S.G. &

Ozcel, M. A. (1998) Sero-diagnosis and epidemiology of visceral leishmaniasis in Turkey. Am. J. Trop. Med. Hyg. 59: 363-369.

79. Katakura, K., Kawazu, S.-I., Naya, T., Nagakura, K., Ito, M., Aikawa, M., Qu, J.-Q., Guan, L.-R., Zuo, X.-

P., Chai, J.-J., Chang, K.-P. and Matsumoto, Y. (1998) Diagnosis of Kala-azar by nested PCR based on amplification of the Leishmania mini-exon gene. J. Clin. Microbiol. 36: 2173-2177.

80. Mensa-Wilmot, K., Garg, N., McGwire, B.S., Lu, H.-G., Zhong, L., Armah, D.A., LeBowitz, J.H., Chang,

K.-P. (1999) Roles of free GPIs in amastigotes of Leishmania Mol. Biochem. Parasitol. 99: 103 – 116. 81. Chen, D.Q.. Lu, H.G. and Chang, K.-P. (1999) Replacement of the gene encoding N-acetylglucosamine-1-

phosphate transferase requires episomal rescue in Leishmania amazonensis. Mol. Biochem. Parasitol. 100: 223-227.

82. Brittingham, A., Chen, G., McGwire, B. S., Chang, K.-P. and Mosser, D. (1999) Interaction of Leishmania

gp63 with cellular receptors for fibronectin. Inf. Immun. 67: 4477-4484. 83. Chen, D.-Q, Kolli, B.K., Yadava, N., Lu, H.-G., Gilman-Sachs, A., Peterson, D.A. and Chang, K.-P. (2000)

Episomal expression of specific sense- and anti-sense mRNAs in Leishmania amazonensis; Modulation of gp63 level in promastigotes and their infection of macrophages in vitro. Inf. Immun. 68: 80-86.

84. Chai J, Chang KP, Zuo X, Yan L, Hou Y, Zhang S, Ruziguli, Jiang W, Zhang L. (1999) [Protective effects of

leishmanial antigens against Leishmania infantum infection in Lagurus lagurus]. Chin. J. Parasitol. Parasit. Dis. [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi.]. 17: 237-40.

85. Akman, L., Ozensoy, S., Ozbel, Y., Turgay, N., Alkan, M.Z., Ozcel, M.A., Culha, G., Ozcan, K., Aksu,

H.A.Z. and Chang. K.-P. (2000) Multi-site DNA polymorphism analyses of Leishmania isolates define their genotype predicting clinical epidemiology of leishmaniasis in a specific region. J. Euk. Microbiol. 47: 545-554.

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83. Qu JQ, Guan LR, Shulidan I, Zuo XP, Chai JJ, Chen SB, Kawazu S, Katakura K, Matsumoto Y, Reed SG,

Chang KP (2000) Rapid screening with a recombinant antigen (rK39) for diagnosis of visceral leishmaniasis using dipstick. Chin. J. Parasitol. Parasit. Dis. 18:155-8.

84. Ma Y, Hu XS, Chang KP (2000) [Diagnosis of kala-azar by rK39 dipstick assay] Chin. J. Parasitol. Parasit.

Dis. [Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi] 18: 252. Chinese. 85. Terabe, M., Kuramochi, T., Ito, M., Hatabu, T., Sanjoba, C., Chang, K.-P., Onodera, T., Matsumoto, Y. (2000)

CD4(+) cells are indispensable for ulcer development in murine cutaneous leishmaniasis. Infect Immun. 68:4574-4577.

86. Guan LR, Qu JQ, Cai JJ, Matsumoto Y, Chang KP. (2001) [Detection of canine visceral leishmaniasis by rK 39

antigen dipstick method] Chin. J. Parasitol. Parasit. Dis. 19: 58. 87. Sah, J.F., Ito, H., Kolli, B.K., Peterson, D.A., Sassa, S. and Chang, K.-P. (2002) Genetic rescue of Leishmania

deficiency in porphyrin biosynthesis creates mutants suitable for analysis of cellular events in uroporphyria and for photodynamic therapy. J. Biol. Chem. 277: 14902-14909.

88. Volf, P., Ozbel, Y.,Akkafa, F., Svobodová, M., Votpka, J., and Chang, K.-P. (2002). Sand Flies (Diptera:

Phlebotominae) in Sanliurfa, Turkey: Relationship of Phlebotomus sergenti with the Epidemic of Anthroponotic Cutaneous Leishmaniasis. J. Med. Entomol. 39: 12-15.

89. McGwire, B.S., O'Connell, W.A., Chang, K.-P. and Engman, D.M. (2002) Extracellular release of the

glycosylphosphatidylinositol (GPI)-linked Leishmania surface metalloprotease, gp63, is independent of GPI phospholipolysis. J. Biol. Chem. 277: 8802-8809.

90. Hatabu T, Matsumoto Y, Kawazu S, Nakamura Y, Kamio T, Lu HG, Chang KP, Hashiguchi Y, Kano S,

Onodera T, Matsumoto Y. (2002) The expression system of biologically active canine interleukin-8 in Leishmania promastigotes. Parasitol. Int. 51: 63-71.

91. McGwire BS, Chang KP, Engman DM.(2003) Migration through the Extracellular Matrix by the Parasitic

Protozoan Leishmania Is Enhanced by Surface Metalloprotease gp63. Infect Immun. 71(2):1008-1010. 92. Rosypal AC, Troy GC, Zajac AM, Duncan RB Jr, Waki K, Chang KP, Lindsay DS. (2003) Emergence of

zoonotic canine leishmaniasis in the United States: isolation and immunohistochemical detection of Leishmania infantum from foxhounds from Virginia. J Eukaryot Microbiol. 2003;50 Suppl:691-3.

93. Chan, M.M.Y., Bulinski, J.C., Chang, K.-P. and Fong. D. (2004) Leishmania amazonensis expressing highly

fluorescent green fluorescent protein (GFP) multimer: Their use for rapid in vitro and in vivo drug screening assays. Parasitol. Res. 89: 266-271.

94. Toz SO, Chang KP, Ozbel Y, Alkan MZ. (2004) Diagnostic value of rK39 dipstick in zoonotic visceral

leishmaniasis in Turkey J Parasitol. 90: 1484-6. 95. Katakura K, Fujise H, Takeda K, Kaneko O, Torii M, Suzuki M, Chang KP and Hashiguchi Y (2004)

Overexpression of LaMDR2, a novel multidrug resistance ATP-binding cassette transporter, causes 5-fluorouracil resistance in Leishmania amazonensis. Febs Lett 561(1-3):207-12.

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96. Thiakaki M., Rohousova I., Volfova V., Volf P , Chang K.-P., Soteriadou K (2005) Sand fly species-specificity of saliva-mediated protective immunity in Leishmania amazonensis-BALB/c mouse mode. Microbes Infect. 7: 760-6.

97. Dutta, S, Ray, D, Kolli, BK, Chang, KP (2005) Photosensitization of Leishmania amazonensis in both

extracellular and intracellular stages with phthalocyanine chloride for photolysis in vitro. Antimicrobial Ag Chemother 49: 4474-84.

98. Sivakumar R, Sharma P, Chang KP, Singh S. (2005) Cloning, expression, and purification of a novel

recombinant antigen from Leishmania donovani. Protein Expr Purif. 6 (1):156-65. 99. Culha G, Uzun S, Ozcan K, Memisoglu HR, Chang KP. (2006) Comparison of conventional and polymerase

chain reaction diagnostic techniques for leishmaniasis in the endemic region of Adana, Turkey. Int J Dermatol. 45(5):569-572.

100. Thiakaki M, Kolli B, Chang KP, Soteriadou K. (2006) Down-regulation of gp63 level in Leishmania

amazonensis promastigotes reduces their infectivity in BALB/c mice. Microbes Infect. 8(6):1455-63. 101. Waki, K., Dutta, S., Ray, D., Kolli, B., Kawazu, S.I., Lin, C.-P. and Chang, K.-P. (2007) Transmembrane

molecules for phylogenetic analysis of pathogenic protists: Leishmania-specific informative sites in hydrophilic loops of trans-ER N-acetylglucosamine-1-phosphate transferase. Eukaryotic Cell 6: 198-210.

102. Mehmet S. Serin, Kayoko Waki, Kwang-Poo Chang, Gonul Aslan, Sahin Direkel, Feza Otag, Begum Kayar,

Fatih Koksal, Gurol Emekdas (2007) Consistence of miniexon polymerase chain reaction–restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes. Diagn. Microbiol. Inf. Dis. 57: 295–299.

103. Kolli, B.K., Costa, J, Chakrabarty, A. and Chang, K.-P.(2008) Leishmania release nucleoside diphosphate

kinase to prevent ATP-dependent apoptosis of infected macrophages Mol Biochem Parasitol 158:163-75. Epub 2007 Dec 25.

104. Dutta, S., Sassa, S., Chang, KP (2008) Leishmania spp.: Delta-aminolevulinate-inducible porphyria by

genetic complementation of incomplete heme biosynthesis. Exp Parasitol 118: 629-36. 105. Dutta,S., Kolli,BK, Tang, A., Sassa, S. and Chang, KP (2008) Transgenic Leishmania model for delta-

aminolevulinate-inducible monospecific uroporphyria: Cytolytic phototoxicity initiated by singlet oxygen-mediated inactivation of functional proteins and its ablation by evacuation of cytosolic uroporphyrin. Eukaryot. Cell 7: 1146-1157.

106. Mehta SR, Huang R, Yang M, Zhang XQ, Kolli B, Chang KP, Hoffman RM, Goto Y, Badaro R, Schooley

RT.(2008) Real time in vivo gfp imaging in a murine leishmaniasis model: a new tool for leishmania vaccine and drug discovery. Clin. Vaccine Immunol. 15, 1764–1770.

107. Kumari S, Samant M, Khare P, Misra P, Dutta S, Kolli BK, Sharma S, Chang KP and Dube A. (2009)

Photodynamic vaccination of hamsters with inducible suicidal mutants of Leishmania amazonensis elicits T cell-transferable immunity against visceral leishmaniasis. Eup. J. Immunol. 38: 1-14.

108. Varela M RE, Lorena Muñoz D, Robledo SM, Kolli BK, Dutta S, Chang KP, Muskus C. (2009) Leishmania

(Viannia) panamensis: An in vitro assay using the expression of GFPfor screening of antileishmanial drug. Exp Parasitol. 122:134-9.

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109. Mehta SR, Zhang XQ, Badaro R, Spina C, Day J, Chang KP, Schooley RT. (2010) Flow cytometric screening for anti-leishmanials in a human macrophage cell line. Exp Parasitol. 126(4):617-20.

110. Wang Y, Chen Y, Xin L, Beverley SM, Carlsen ED, Popov V, Chang KP, Wang M, Soong L (2011) The

differential microbicidal effects of human histone proteins H2A and H2B on Leishmania promastigotes and amastigotes. Infect Immun. 79(3):1124-33.

111. Working Group on Research Priorities for Development of Leishmaniasis Vaccines, Costa CH, Peters NC,

Maruyama SR, de Brito EC Jr, Santos IK, et al. (2011) Vaccines for the leishmaniases: proposals for a research agenda. PLoS Negl Trop Dis. 5(3):e943.

112. Dutta, S., Ongarora, B.G., Li, H., Vicente, MGH, Kolli, BK and Chang, KP. (2011) Intracellular targeting

specificity of novel phthalocyanines assessed in a host-parasite model for developing potential photomedicine. PLoS ONE 6(6):e20786.

113. Dutta S, Waki K, Chang KP. (2012) Combinational Sensitization of Leishmania with Uroporphyrin and

Aluminum Phthalocyanine Synergistically Enhances their Photodynamic Inactivation in vitro and in vivo. Photochem Photobiol. 88(3):620-5.

114. Dutta, S, Chang, C, Kolli, BK, Sassa, S, Yousef, Showe, M, Showe, L, and Chang, KP. (2012) Delta-

aminolevulinate-induced host-parasite porphyric disparity for selective photolysis of transgenic Leishmania in the phagolysosomes of mononuclear phagocytes: A potential novel platform for vaccine delivery Euk Cell 11: 430-441.

115. Kaur J, Dutta S, Chang KP, Singh N A (2013) Member of the Ras oncogene family, RAP1A, mediates

antileishmanial activity of monastrol. J Antimicrob Chemother. 68:1071-80. 116. Jecna, L, Dostalova, A, Wilson, R, Seblova, V, Chang, KP, Bates, PA, Volf, P (2013) The role of surface

glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies. Parasitology 140:1026-32.

117. Alam MZ, Nakao R, Sakurai T, Kato H, Qu JQ, Chai JJ, Chang KP, Schönian G, Katakura K. (2014) Genetic

diversity of Leishmania donovani/infantum complex in China through microsatellite analysis. Infect Genet Evol. 22C:112-119.

118. Shio MT, Christian JG, Jung JY, Chang KP, Olivier M. PKC/ROS-Mediated NLRP3 Inflammasome Activation

Is Attenuated by Leishmania Zinc-Metalloprotease during Infection. PLoS Negl Trop Dis. 2015 Jun 26;9(6):e0003868.

119. Al-Qahtani A, Saad Alkahtani S, Kolli BK, Tripathi P, Dutta S,. Al-Kahtane AA, Jiang XJ, Ng DKP, Chang

KP (2016). Amino-phthalocyanine-mediated photodynamic inactivation of Leishmania tropica. Antimicrob Ag Chemther. 2016 Mar 25;60(4):2003-11.

120. Chang KP, Kolli BK & New Light Group (2016): New "light" for one-world approach toward safe and effective control of animal diseases and insect vectors from leishmaniac perspectives. Parasit &Vectors. 2016 Jul 13;9(1):396. doi: 10.1186/s13071-016-1674-3. Review. PMID: 27412129.

121. Mishra AK, Singh N, Agnihotri P, Mishra S, Singh SP, Kolli BK, Chang KP, Sahasrabuddhe AA, Siddiqi MI, Pratap JV (2017). Discovery of novel inhibitors for Leishmania nucleoside diphosphate kinase (NDK) based on its structural and functional characterization. J Comput Aided Mol Des. 2017 May 27. doi: 10.1007/s10822-017-0022-9. [Epub ahead of print]. PMID: 28551817.

40

122. Viana SM, Celes FS, Ramirez L, Kolli B, Ng DKP, Chang KP, de Oliveira CI (2018) Photodynamic Vaccination of BALB/c Mice for Prophylaxis of Cutaneous Leishmaniasis Caused by Leishmania amazonensis.. Front Microbiol. 2018 Feb 6;9:165. doi: 10.3389/fmicb.2018.00165

123. Kwang Poo Chang, Bala K. Kolli, Chia-Kwung Fan, Dennis K. P. Ng, Clarence T. T. Wong, Laura Manna, Raffaele Corso, Neng-Yao Shih, Robert L. Elliott, X. P. Jiang, Shin-Hong Shiao, Guo-Liang Fu (2018) "Progress toward development of photodynamic vaccination against infectious/malignant diseases and photodynamic mosquitocides", Proc. SPIE 10479, Light-Based Diagnosis and Treatment of Infectious Diseases, 1047912 (8 February 2018); doi: 10.1117/12.2281437; https://doi.org/10.1117/12.2281437

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Under revision/preparation:

124. Kayoko Waki, Leyla Akman, Reha Toydemir, Seray Osenzoy-Toz, Yusuf Ozbel, Gulnaz Culha, Henk Schallig, Max Grogl, Mehdi Mohebali, Hatice Ertabaklar, Petr Volf, Milena Svobodova, Ikram Guizani and Kwang-Po0 Chang. Dominant heterozygous genotype of Leishmania tropica in cyclic epidemics of anthroponotic cutaneous leishmaniasis of Asia Minor. (Submitted).

125. Chang. Dominant heterozygous genotype of Leishmania tropica in cyclic epidemics of anthroponotic cutaneous leishmaniasis of Asia Minor. (Submitted).

126. Kolli, BK, Dutta, S., Cedeno, D, Francis, K. and Chang, KP. (2011) Cytolysis of genetically and chemically photosensitized Leishmania by luciferase-generated luminescence. J. Biol. Chem. (In preparation). 127. Kolli BK, Dutta, S, Soteriadou, K and Chang KP. Transfection of Leishmania to over-express nucleoside diphosphate kinase increases their in vivo activity to recruit macrophages and other mononuclear cells in rodents (In preparation). 128. Kolli, B., Chang, K.-P. Activities of nucleoside diphophate kinase and its genomic polymorphism in New World and Old World Leishmania subgenus (In preparations). 129. Lu, H.-G., Chen, D.-Q. and Chang, K.-P. The absence of apparent phenotypic changes with null mutation of a highly conserved gene encoding oxidoreductase in Leishmania amazonensis. Episomal and chromosomal expression of foreign genes at this site. (In preparation).

42


PUBLICATIONS Reviews:

1. Chang, K.-P. (1983) Molecular and biochemical mechanisms of intracellular symbiosis in leishmaniasis Int. Rev. Cytol. Suppl. 14: 267-302.

2. Chang, K.-P. (1983) Leishmania-macrophage cellular and molecular interactions in vitro. Microbiology

380-383.

3. Chang, K.-P., Chaudhuri, G. & Fong D. (1990) Molecular determinants of Leishmania virulence. Ann. Rev. Microbiol. 44:499-529.

4. Chang, K.-P. & McGwire, B.S. (2002) Molecular determinants and regulation of Leishmania virulence

Kinetoplastid Biology and Disease 1:1-7. (http://www.kinetoplastids.com/content/1/1/1).

5. Chang, K.-P., Reed, S.G., McGwire, B.S. & Soong, L. (2003) Leishmania model for microbial virulence: The relevance of parasite multipliction and pathoantigenicity. Acta Trop. 85: 375-90.

6. Chang KP. (2014) Vaccination for Disease Prevention and Control: The Necessity of Renewed Emphasis and New Approaches. J Immunol Immunotech. 2014 1(1): 1-4.

7. Lun ZR, Wu MS, Chen YF, Wang JY, Zhou XN, Liao LF, Chen JP, Chow LM, Chang KP (2015). Visceral Leishmaniasis in China: an Endemic Disease under Control. Clin. Microbial. Rev. 28 (4), 987-1004.

Book Chapters: 1. Chang, K.-P. & Fish, W.R. (1983) Leishmaniasis. In In vitro cultivation of pathogenic protozoa of man

and domestic animals, Ed. J. B. Jensen, CRC Uniscience pp. 111-153. 2. Chang, K.-P., Dasch, G. & Weiss, E. (1984). Endosymbionts of fungi and invertebrates other than insects,

In: Bergey’s Manual of Systematic Bacteriology, Ed. N.R. Krieg, Vol. I, 853-863, William & Wilkins, Baltimore.

3. Dasch, G., Chang, K.-P. & Weiss, E. (1984) Endosymbionts of insects. In: Bergey’s Manual of Systematic

Bacteriology, Ed. N.R. Krieg, Vol. I, 811-830, Williams & Wilkins, Baltimore. 4. Weiss, E., Dasch, G., & Chang, K.-P. (1984) Wolbacheae. In: Bergey’s Manual of Systematic

Bacteriology, Ed. N.R. Krieg, Vol. I, 711-717, Williams & Wilkins, Baltimore. 5. Chang, K.-P., Fong, D and Bray, R.S. (1985) Biology of Leishmania and leishmaniasis. In: Leishmaniasis,

Eds. K.-P. Chang and R.S. Bray, Elsevier Biomedical Press, Amsterdam, pp. 1-27. 6. Chang, K.-P. & Hendricks, D.L. (1985) Laboratory maintenance of leishmaniae. In: Leishmaniasis, Eds.

K.-P. Chang & R.S. Bray, Elsevier Biomedical Press, Amsterdam, pp. 213-246. 7. Chang, K.-P., Nacy, C.A. & Pearson, R.D. (1986) Intracellular parasitism of macrophages in leishmaniasis:

In vitro systems and their applications. In: Phagocytosis and cytotoxicity of macrophages Methods in Enzymol. 132: 603-625.

8. Chang, K.-P. (1990) Cell biology of Leishmania. In: Modern Parasite Biology-Cellular, immunological

and molecular aspects. Ed. D.J. Wyler, W.H. Freeman Co., New York, Chapter 5 pp. 79-90 9. Singh, S. & Chang, K.-P. (1996) Genetic heterogeneity among Indian Leishmania donovani isolates. In

Indian Kala-Azar. Ed. S. Sundar, Banaras Hindu University, Varanasi, pp. 197-204.

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PUBLICATIONS Book chapters (Con’t): 10. Chang, K.-P. (1995) Leishmaniasis. In: Encyclopedia of human biology. Ed. R. Dubelcco, Academic

Press, San Diego, Vol. 5, pp. 275-281.

11. Chang, K.-P. (1997) Leishmaniasis. In: Encyclopedia of human biology. 2nd Edition, Ed. R. Dubelcco,

Academic Press, San Diego. Vol. 5, pp. 275-281. 12. Chang, K.-P. (1999) Leishmaniasis in the Old World. In: Epidemiology and control of leishmaniasis in

central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp. 1-14.

13 .Katakura, K., S.-I. Kawazu, T. Naya, K. Nagakura, M. Ito, M.Aikawa, J.-Q. Qu , L.-R. Guan , X.-P. Zuo ,

J.-J. Chai, K.-P. Chang and Y. Matsumoto (1999) PCR-Based Diagnosis of Kala-Azar in Xinjiang-Uigur Autonomous Region, China. In: Epidemiology and control of leishmaniasis in central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp.37-44.

14. Nakakura, K., T. Naya, K. Katakura, J.-J. Chai, S.-I. Kawazu, M.Ito, J.-Q. Qu, L.-R. Guan, K.-P. Chang, M.

Aikawa and Y. Matsumoto (1999) IFA is a Powerful Tool to Monitor the Progression of Visceral Leishmaniasis In: Epidemiology and control of leishmaniasis in central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp. 45-47.

15. Qu, J.-Q., Q. Feng, I. Shulidan, X.-P. Zuo, J.J. Chai, S.-I. Kawazu, K. Katakura, Y. Matsumoto, S. G. Reed

and K.-P. Chang (1999) Serodiagnosis of Visceral Leishmaniasis in Northwestern China: Rapid Screening with a Recombinant Antigen (rK39) in Dipstick Format and Its Specificity vs.Promastigote Antigens In: Epidemiology and control of leishmaniasis in central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp.48-56.

16. Nagakura, K., T. Naya, K. Katakura, J.-J. Chai, S.-I. Kawazu, M. Ito, J.-Q. Qu, L.-R. Guan, K.-P. Chang,

M. Aikawa and Y. Matsumoto (1999) Parasites Detection Using Biopsies from Chinese Patients with Visceral Leishmaniasis In: Epidemiology and control of leishmaniasis in central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp.75-77

17. Kawazu, S.-I., K. Katakura, K. Nagakura. T. Naya, Mamoru Ito, Y. Nakai, M. Aikawa, Jing-Qi Qu, L.R.

Guan, X.-P. Zuo, J.-J. Chai, K.-P. Chang and Yoshitsugu Matsumoto (1999) Zeta-crystallin/NADPH oxidoreductase homologue (p36) gene sequences for analysis of the phylogenetic relationships among Leishmania spp In: Epidemiology and control of leishmaniasis in central Eurasia, Research report series No. 1 (1996-1998), Ed. Y. Matsumoto, Int. Press Edit. Cent. Inc., Tokyo, pp.78-82.

18. Chang, K.-P., Akman L. and Nielson, J. (1999) Leishmania virulence and genetic heterogeneity, Ed. O.

Omeish, Clin. Dermatol. 17: 269-273.

19. Motta, M.C.M., De Souza, W. and Chang, K.-P. (2002) Endosymbiosis of beta-Proteabacteria with trypanosomatid protozoa. In: Symbiosis, Ed. J. Seckbach, Hebrew University Press, Jerusalem (In press).

20. Chang K-P (2002) Leishmaniases. In: Encyclopedia of Life Sciences, vol. 11, pp. 10-18. London: Nature

Publishing Group. http://www.els.net/

21. Chang K-P (2005) Leishmaniases. In: Encyclopedia of Life Sciences, doi: 10.1038/npg.els.0003824, John Wiley & Sons, Ltd. http://www.els.net/

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PUBLICATIONS

Book chapters (Con’t):

22. Chang, KP. (2006) Molecular and immunological aspects of Leishmania relevant to control and treatment of

leishmaniasis and other diseases in China [English with translation in Chinese]. In: Leishmaniasis and Phlebotomine sandflies in Xinjiang Uygur Autonomous region of the People’s Republic of China, Eds. J.J. Chai and L.R. Guan, Xinjiang People’s Publishing Co, pp. 51-79.

23. Chang KP, Kolli BK, Dutta S, Waki K. (2007) Leishmaniac jihad: Let the evils fight the devils. Proceed Int Conf Environ Parasitol/Commun Heal Care Initiat. Sect 1-4, pp. 13-16, Ed. S Prakash, Allied Publish, N Delhi, India.

24. Chang KP (2012) Leishmaniases. In: Encyclopedia of Life Sciences, DOI: 10.1002/9780470015902.a0001954.pub3, John Wiley & Sons, Ltd. http://www.els.net/

25. Chang, KP (2013) Forward In: “Drug Resistance in Leishmania Parasites, Consequences, Molecular

Mechanisms and Possible Treatments”, Eds. Ponte-Sucre, Alicia; Diaz, Emilia; Padrón-Nieves, Maritza XIV, Springer.

26. Chang KP (2014) Leishmaniases. In: Encyclopedia of Life Sciences, 3rd Edition.

27. Chang KP (2017) Overview of leishmaniasis with special emphasis on kala-azar in South Asia. In: Neglected

tropical diseases, Eds. Sunit K. Singh, Springer (Accepted December, 2016, Published February, 2018).

45


PUBLICATIONS Symposium and Workshop Papers: 1. Chang, K.-P. (1975) Haematophagous insects and haemoflagellates as hosts for prokaryotic endosymbiotes.

Symp. Soc. Exp. Biol. 29: 407-428. 2. Chang, K.-P. and Dave, C. (1980) Modulation of polyamine biosynthesis enzymes by bacterial

endosymbionts in trypanosomatid protozoa. In: Int. Proc. Endosymb. Cell Res., Eds. W. Schwemmler & H. Schenk, Walter de Gruyter, Berlin, 1: 349-360.

3. Chang, K.-P. & Fong, D. (1983) Cell biology of host-parasite membrane interactions in leishmaniasis.

Cytopathology of Parasitic Diseases. Ciba Foundation Symp. 99: 113-131. 4. Fong, D. and Chang, K.-P. (1986) Changes in tubulin mRNAs during differentiation of a parasitic

protozoan, Leishmania mexicana. Ann. N.Y. Acad. Sci. 466: 129. 5. Chang, K.-P., Kink, J.A., and Detke, S. (1987) Tunicamycin-resistant Leishmania mexicana: Increased N-

acetylglucosaminyltransferase, its gene and virulence. In: Molecular Strategy of Parasite Invasion UCLA Winter Symposium., NS 42: 77-86.

6. Chang, K.-P. (1987) Molecular pathogenicity of Leishmania in intracellular parasitism of macrophages:

Association of virulent phenotype with increased expression of a major membrane glycoprotein and microsomal N-acetylglucosaminyltransferase. In: Host-parasite cellular and molecular interactions in protozoal infections, Eds. K.-P. Chang & D. Snary, Springer-Verlag, Heidelberg NATO ASI Series Vol. H11, 107-116.

7. Chaudhuri, G. & Chang, K.-P. (1987) Acid protease activity of Leishmania gp63 and its possible role in

virulence. In: Host-parasite cellular and molecular interactions in protozoal infections, Eds. K.-P. Chang & D. Snary, Springer-Verlag, Heidelberg, NATO ASI Series H11, 169-173.

8. Kahl, L.P., Chang, K.-P., & Liu, F.Y. (1988) the 63kDa promastigote protease of Leishmania: Assessment

of host-protection in BALB/c mice using liposomes as adjuvant. In: Leishmaniasis: The new strategies for control, Ed. D.T. Hart, NATO ASI Series. A, Vol 163, pp 417-422.

9. Olafson, R.W.,Chang, K.-P., & Ferguson, A.J.M. (1988) Biochemical characterization of Leishmania gp63

as an acid protease. In: Leishmaniasis: The new strategies for control, Ed. D.T. Hart, NATO ASI Series. A, Vol 163, pp. 619-625.

10. Chang, K.-P., Chaudhuri, G., Chaudhuri, M., Katakura, K., Detke, S., & Zhang, R. (1989) Molecular

determinants of Leishmania virulence: Surface metallo-proteinase, N-glycosylation and DNA amplification. In: Current Perspectives in Parasitic Diseases, Ed. R.C. Ko, Hong Kong, University Press, Hong Kong, pp.111-128.

11. Chang, K.-P. Chaudhuri, G., Chaudhuri, M. & Katakura, K. (1990) Gp63 as a surface metalloproteinase in

Leishmania virulence: Molecular determinants of proteolytic activity and N-glycosylation. In: Immune Recognition and Evasion: Molecular Aspects of Host-Parasite Interaction, Eds. Lex H.T. Van der Ploeg, C.R., Cantor and H.J. Vogel, P & S Biomedical Science Symposium, Academic Press, New York, pp. 279-293.

12. Chang, K.-P.(l993) Evolution and regulation of Leishmania virulence. Proc. CSIR Symp., Current Trends in

Leishmania Research, CSIR Press, New Dehli, pp. 15-28.

46


PUBLICATIONS - Abstracts: 1. Chang, K.-P. (1968) Studies on the structure, ultrastructure and cytochemistry of the mycetome of the pear

psylla. Proc. Ent. Soc. Ont. 99, 81. 2. Tuan, R.S. & Chang, K.-P. (1974) Isolation and characterization of DNA from symbiotic and aposymbiotic

strains of Blastocrithidia culicis. Proc. 3rd Int. Cong. Parasitol. 3, 1453. 3. Chang, K.-P., Chang, C.S., Tuan, R.S. & Sassa, S. (1974) Ultrastructure and heme biosynthesis of

symbiotic bacteria in two species of hemoflagellates. Proc. 3rd Int. Congr. Parastiol. 2, 26. 4. Dwyer, D.M. & Chang, K.-P. (1975) Surface saccharide alterations in a flagellate protozoan mediated by

bacterial endosymbiotes. J. Cell Biol. 67, 102a. 5. Dwyer, D.M. & Chang, K.-P. (1975) Surface membrane terminal saccharides of Leishmania donovani

promastigotes. J. Protozool. 22, 37-8A. 6. Chang, K.-P. & Dwyer, D.M. (1975) Leishmania donovani-hamster macrophage interaction in vitro. J.

Parasitol. 61 (Suppl.), 71. 7. Meshnick, S., Chang, K.-P. & Cerami, A. (1976) The effects of hemin on salivarian trypanosomes. J.

Parasitol. 62 (Suppl.), 33. 8. Dave, C., Chang, K.-P. & Cheng, Y.C. (1976). Activity of polyamine biosynthesis enzymes in

trypanosomatid protozoa. J. Parasitol. 62 (Suppl.), 32. 9. Chang, K.-P. & Steiger, R.F. (1976) Anti-trypanosome activity of methylgloxal bis (guanylhydrazone). J.

Parasitol. 62 (Suppl.), 32. 10. Chang, K.-P. (1977) Leishmania infection of human skin fibroblasts in vitro. Am. Soc. Trop. Med. Hyg.

25th Ann. Meeting. 11. Chang, K.-P. (1977) Leishmania donovani-hamster macrophage surface interactions in vitro: Quantitation

and characterization of host-parasite attachment using tritium-labeled promastigotes. Int. Cong. Protozool. 5, 463.

12. Chang, K.-P. (1977) Survival and growth of Leishmania donovani in human and murine mononuclear

phagocytes. J. Parasitol. 63 (Suppl.), 43. 13. Chang, K.-P. (1977) Endosymbiosis in trypanosomatid flagellates. Int. Congr. Protozool. 5, 438. 14. Chang, K.-P. & Chiao, J.W. (1978) Visceral leishmaniasis in congenitally athymic mice. Ann. Meet. Am.

Soc. Microbiol. 15. Chang, K.-P. & Chiao, J.W. (1978) Comparison of in vitro leishmanicidal activity in macrophages mediated

by lymphocytes from normal and nude mice. IV Int. Cong. Parasitol. 16. Chang, K.-P. (1978) Phagocytosis and intracellular digestion of leishmanial amastigotes by human

polymorphonuclear phagocytes. J. Protozool. 25, 20A. 17. Chang, K.-P. (1978) Lysosome-phagosome fusion in polymorphonuclear phagocyte-Leishmania donovani

interactions in vitro. J. Parasitol. 64 (Suppl.), 84.

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PUBLICATIONS - Abstracts: (Con’t) 18. Chang, K.-P. (1979) Interactions of leishmanias with mammalian cells in vitro, WHO Workshop on in vitro

cultivation of pathogens of tropical diseases, ILRAD, Nairobi, Kenya. 19. Chang, K.-P. (1980) Endocytosis of FITC-dextran by leishmania-infected macrophages in vitro:

Fluorometry of pinocytosis, lysosome-phagosome fusion and intralysosomal pH. 3rd Int. Symp. Biochemistry of Parasites & Host-Parasite Relationships, Beerse, Belgium.

20. Chang, K.-P. (1981) A complete development cycle of leishmanias in vitro: Some biological and

biochemical studies. Ann. Meet. Am. Soc. Trop. Med. Hyg. 21. Chang, K.-P. (1981) Surface antigen/glycoprotein-mediated binding of Leishmania donovani to hamster

macrophages. Ann. Meet. Soc. Parasitol. 22. Fong, D. & Chang, K.-P. (1981) Protease activities of a parasitic protozoan, Leishmania mexicana. J. Cell

Biol. 9, 43a. 23. Chang, K.-P. & Fong, D. (1982) Identification of surface antigenic proteins of Leishmania mexicana

amazonensis. J. Protozool. 29, 295. 24. Fong, D., Chan, M.M., Chang, K.-P. (1983) Stage-specific monoclonal antibodies specific to Leishmania

mexicana. 5th Int. Cong. Immunol. 806-810. 25. Rivas, L. and Chang, K.-P. (1983) Intraparasitophorous vacuolar pH of leishmania-infected macrophages.

Biol. Bull. 165, 536. 26. Anaya-Velazquez, L.F. & Chang, K.-P. (1983) Phagocytosis and intralysosomal killing of Leishmania

mexicana by Entamoeba histolytica. Biol. Bull. 165, 534. 27. Chang, C.S. & Chang, K.-P. (1984) Heme biosynthesis in Leishmania-macrophage interactions in vitro.

ASBC/AAI Minisymposium KM2 Biochemistry of Protozoa. 28. Chang, K.-P. & Prachayasittikul, V. (1984) Nucleotide heterogeneity of restriction fragments from

kinetoplast DNAs of three Chinese Leishmania species. Eroup. Multicolloq. Parasitol. IV, Izmir, Turkey. 29. Prachayasittikul, V. & Chang, K.-P. (1984) Kinetoplast DNA heterogeneity and homogeneity of three

Chinese Leishmania species determined by dot-blot hybridization. Ann. Meet. Am. Soc. Parasitol. 30. Chang, K.-P. & Chang, C.S. (1984) Leishmanial infection of macrophages in vitro: A quest for biological

and biochemical basis. XI. Int. Cong. Trop. Med. Malaria, Calgary, Canada. 31. Chang, K.-P. (1985) Phagocytosis of leishmanias by macrophages in vitro. VII Int. Cong. of Protozoo.,

Nairobi, Kenya. 32. Chang, K.-P. (1985) Stage-specific molecules of Leishmania. Impact of modern genetic, biochemical and

immunological techniques on human leishmaniasis, Coltswald, UK p. 6. 33. Detke, S., Kink, J.A. & Chang, K.-P. (1986) Characterization of amplified DNA from tunicamycin resistant

Leishmania mexicana. J. Cell. Biochem. 10a (Suppl.), 129. 34. Chang, K.-P., Kink, J.A., Detke, S., & Chang, C.S. (1986) Molecular mechanism of parasite virulence in

leishmaniasis: Possible role of a 63 kDa membrane glycoprotein and glycoglytransferase gene amplification. 1st SCBA Int. Symp., p. 37.

48


PUBLICATIONS - Abstracts: (Con’t) 35. Chang, K.-P., Chaudhuri, G., Peng, Y., Detke, S., Chang, C.S. & Fong, D. (1986) Leishmania gp63 as a major

surface glycoprotein: Molecular heterogeneity and possible biological functions, NATO ARW “Host-parasite molecular recognition and interactions in protozoal infections”, Proc. p. 6.

36. Chaudhuri, G. & Chang, K.-P. (1986) Acid protease activity of leishmania gp63: Possible role in virulence,

NATO ARW “Host-parasite molecular recognition and interactions in protozoal infections”, Proc., p. 7. 37. Detke, S., Kink, J.A., Chaudhuri, G. & Chang, K.-P. (1986) Structure of the amplified DNA in

tunicamycin-resistant Leishmania mexicana. NATO ARW “Host-parasite molecular recognition and interactions in protozoal infections”, Proc. p. 10.

38. Kink, J.A. & Chang, K.-P. (1986) N-acetylglucosaminyltransferase gene amplification associated with

virulent phenotype of Leishmania mexicana. NATO ARW “Host-parasite molecular recognition and interactions in protozoal infections”, Proc., p. 24.

39. Chang, K.-P. (1987) Cellular and molecular aspects of extrachromosomal genetic elements in parasitism

and symbiosis. Symp. Recent Adv. Biol. & Med. Sci., Taipei, Taiwan, Program & Abstract, p. 14. 40. Chang, K.-P. (1987) Molecular determinants of Leishmanial virulence. NATO Adv. Study Inst. on

Leishmaniasis, Program & Abstracts, p. 23. 41. Chang, K.-P. (1988) N-glycosylation of a dominant surface antigen in its diagnostic and prophylactic

potential for leishmaniasis. 2nd SCBA Int. Symp., San Francisco. 42. Chang, K.-P. (1989). Molecular determinants of Leishmania virulence. Int. Symp. Mol. Parasitol., Acad.

Sinica, Taipei, Taiwan. Program and abstract, p. 22. 43. Chang, K.-P. (1989). Molecular determinants of Leishmania virulence. VIII Int. Cong. Protozool.,

Tsukuba, Japan, p. 59. 44. Chang, K.-P. (1989) Molecular determinants of Leishmania virulence. 2nd SE Asian Symposium

Parasitology & Modern Medicine, Molecular Basis of Pathogenesis in Parasitic Diseases, Hong Kong, p.8.

45. Chaudhuri, G., Chaudhuri, M., Latshaw, S.P., Moberley, L., Kemp, R.G., Chang, K.-P. (1989). Biochemical characterization of gp63 as a metallo-proteinase from Leishmania

mexicana amazonensis. Gordon Research Conference, New Hampshire. 46. Katakura, K., Peng, Y., Liu, X., Pithawalla, R., Chaudhuri, M., Detke, S., Chang, K.-P. (1989). DNA

amplification in five species of tunicamycin-resistant Leishmania: Extrachromosomal circles of different sizes with a 20-kb consensus DNA region and over-expression of N-acetylglucosamine-1-phosphate transferase protein. Gordon Research Conference, New Hampshire.

47. Chang, K.-P. (1990) Searching for the molecular determinants of Leishmania virulence - A rational

approach to the control of parasitic diseases. Proc. Symp. Med. Parasitol., Shanghai, pp. 47-48. 48. Chaudhuri, G., Chaudhuri, M., Latshaw, S.P., Moberley, L., Kemp, R.G., Chang, K.-P. (1990). Surface

metallo-proteinase (gp63) of Leishmania mexicana amazonensis-specific primary structure, extended substrate binding site and implication of metallo-enzyme-conserved residues. AAAS Annual Meeting-Biology of Partasitism Seminar, New Orleans.

49


PUBLICATIONS - Abstracts: (Con’t) 49. Katakura, K., Pithawalla, R., Peng, Y., Chaudhuri, G., Chaudhuri, M., Chang, K.-P. (1990). DNA

amplification in tunicamycin-resistant Leishmania spp.: Consensus region in extrachromosomal circles of variable sizes, mutiple transcripts with translational activity and over-production of N-acetylglucosamine-1- phosphate transferase. AAAS Annual Meeting-Biology of Parasitism Seminar, New Orleans.

50. Chang, K.-P. (1990). Surface antigens of Leishmania. 3rd SCBA Int. Symp. Inf. Dis., Hong Kong. 51. Chang, K.-P. (1991) Molecular biology of Leishmania virulence. Proc. Soc. Protozool., Bozman,

Montana, p. 23. 52. Liu, X. & Chang, K.-P. (1991) Leishmania DNA sequence responsible for tunicamycin-resistance. Proc.

Am. Soc. Trop. Med. Hyg. 40, 107. 53. Chang, K.-P. Du, Y. & Liu, X. (1991) Transfection of Leishmania with gp63 metalloproteinase gene and its

expression in the transfectants. Proc. Am. Soc. Trop. Med. Hyg. 40, 108. 54. Chang, K.-P. (1992) Cellular and molecular biology of Leishmania virulence. Proc. Int. Symp. Curr.

Trend. Leishmania Res., Calcutta, p. 8. 55. Chang, K.-P. (1992) Chromosomal and extrachromosomal genes in Leishmania virulence. Symp. Mol.

Parasitol. 4th Int. SCBA Symp. P. 256. 56. Du, Yubin, Chang, K.-P. (1992) PCR amplification of bacterial pre-rRNA genes in endosymbionts-

containing Crithidisa spp., V Int. Coll. Endocytobiology Symbiosis, Kyoto, Japan. P. 73. 57. McGwire, B.S. & Chang, K.-P. (l993) Functional analysis of Leishmania metalloproteinase (gp63) by site-

specific mutagenesis. MBL Mol. Parasitol. Meet. No. 185. 58. Liu, X. & Chang, K.-P. (l993) An overexpressed gene coamplified with tunicamycin-resistance marker is a

_-crystallin/NADPH-oxidoreductase homolog constitutively expressed in Leishmania spp. MBL Mol. Parasitol. Meet. No. 179.

59. Lu, H.G. & Chang, K.-P. (l993) Extrachromosomal rescue of lethal null mutants of N-acetylglucosamine-1-

phosphate transferase gene in Leishmania. MBL Mol. Parasitol. Meet. No. 180. 60. Lu, H.-G. and Chang, K.-P. (l993) Heterogeneity of kinetoplast minicircles is independent of chromosomal

DNA alterations in Leishmania amazonensis. MBL Mol. Parasitol. Meet. No. 176. 61. Kojo, Mensa-Wilmot, Tucker, S., McGwire, B.S., Chang, K.-P. and LeBowitz, J. (l993) Expression of a

glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei in Leishmania causes secretion of gp63: Implications for the topology of glycosylPhosphatidyl inositol biosynthesis in vivo. MBL Mol. Parasitol. Meet. No. 272.

62. Lu, H.G., Zhong, Li, Chai, T. Z., Qu, J.Q. & Chang, K.-P. (l993) Genotype and phenotype heterogeneity of

Leishmania isolates from western China. Joint Meet. Am. Soc. Trop. Med. & Hyg./Parasitol. P. 373. 63. Yasinzai, M. M. & Chang, K.-P. (l993) Characterization of parasites causing cutaneous and visceral

Leishmania from Pakistan. Joint Meet. Am. Soc. Trop. Med. & Hyg./Parasitol. P. 380. 64. Chang, K.-P. (l993) Molecular basis of Leishmania virulence. Joint Meet. Am. Soc. Trop. Med. &

Hyg./Parasitol. P. 417.

50


PUBLICATIONS - Abstracts: (Con’t) 65. Chang, K.-P. (l993) New perspectives of an ancient disease along the “Silk Roads” - Leishmaniasis. XXVI

Congr. Turk. Soc. Microbiol. P. 429. 66. Kawazu, S.-I. and Chang, K.-P. (1994) Transcriptional mapping of two closely linked heterogenous genes

expressed constitutively in Leishmania. 8th Intl. Congr. Parasitol. Abst. 2: 264. 67. Aksu, H.S.Z., Chang, K.-P., Reed, S.G., Ak, M., Ozbel, Y. and Ozcel, M.A. (1994) Serodiagnostic potential

of specific antigens to discirminate cutaneous and visceral leishmaniasis due to genetically heterogenous isolatres. 8th Intl. Congr. Parasitol. Abst. 2: 291.

68. Yasinzai-Masoom, M. and Chang, K.-P. (1994) Leishmaniasis in Pakistan: Parasite characterization and

chemotherapy. 8th Intl. Congr. Parasitol. Abst. 2: 387. 69. Singh, S. and Chang, K.-P. (1994) Genetic heterogeneity of Indian Leishmania donovani isolates. 8th Intl.

Congr. Parasitol. Abst. 2: 344. 70. Guan, L.R., Yang, T.Q., Xu, Y.X., Qu, J.Q., Zho, X.P., Wang, G., Lu, H.G., Zhing, L. and Chang, K.-P. (1994) Genetically different parasites cause human and gerbil cutaneous

leishmaniasis in northwest China. 8th Intl. Congr. Parasitol. Abst. 2: 344. 71. Chang, K.-P. (1994) Hypothetical consideration of Leishmania virulence and control of leishmaniasis. 8th

Intl. Congr. Parasitol. Abst. 1: 59. 72. McGwire, B., Lu, H.G., Zhong, L., Du, Y.B., Kawazu, S.-I., Yang, X.X. and Chang, K.-P. (1994)

Extrachromosomal expression of Leishmania surface zinc proteinase (gp63) and their site-specific mutants. 8th Intl. Congr. Parasitol. Abst. 1: 57.

73. Reed, S.G., Burns, J.M., Chang, K.-P., Chung, P. and Badaro, R. (1994) Cloning and expression of a

Leishmania donovani specific antigen gene and clinical application of its encoded protein. 8th Intl. Congr. Parasitol. Abst. 1: 56.

74. Qu, J.Q., Boa, Y.F., Wuli, G., Reed, S.G. and Chang, K.-P. (1995) Serodiagnosis of leishmaniasis in

Northwest China using a recombinant antigen. Proc. Chin. Protozool. Soc. 8, 114. 75 Guan, L.R., Xu, Y.X., Yang, Y.C., Qi, J.Q., Tsio. X.P., Wang, G., Chai, J.J., Lu, H.G., Li, C. and Chang,

K.-P. (1995) Discovery of cutaneous leishmaniasis due to Leishmania infantum in Nothern Xinjiang, China. Proc. Chin. Protozool. Soc. 8, 115.

76. Chang, K.-P. (1995) A personal view from a leishmaniac on the importance of cooperative and

multidisciplinary research on parasitic diseases in Turkey. Turk. Natl. Congr. Parasitol. 77. Chang, K.-P., Lu, H.G., Wang, R.Q., Aksu, H.A.Z., Culha, G., Ozcan, K., Ozbel, Y., Ozensoy, S., Alkan, Z.

and Ozcel, M.A. (1995) Grouping of Turkish Leishmania isolates by nuclear and kinetoplast DNA polymorphisms. Turk. Natl. Congr. Parasitol.. P. 8

78. Chang, K.-P. (1995) New perspectives of an ancient disease along the “Silk Road” - Leishmaniasis. 1st Intl.

Congr. Fever/Environ. P. 1. 79. Chang, K.-P. (1995) Genetic heterogeneity of Leishmania and their virulence in the strategies of control for

leishmaniasis. Pan Arab Seminar Zoonost. Dis. P. 38-39. 80. Chang, K.-P. (1996) Genetic heterogeneity of Leishmania and their virulence in leishmaniasis: Strategies of

control. Plenary Lecture, Symposium on leishmaniasis. Glob Meet. Parasitic Diseases. P. 3.

51


PUBLICATIONS - Abstracts: (Con’t) 81. Masoom-Yasinzai, M. and Chang, K.-P. (1996) Leishmaniasis in Pakistan: Development of potent

chemotherapeutic agent. Glob Meet. Parasitic Diseases. P. 39. 82. Chang, K.-P. (1996) Leishmania determinants of virulence and genetic heterogeneity: Strategies of control

for leishmaniasis. Leishmaniasis State-of-The-Art Session. 14th Int. Cogr. Trop. Med. P. 238. 83. Kawazu, S-I., Lu, H.G., Zhong, L., Ito, H., Kamio, T. and Chang, K.-P. (1996) Stage-independent co-

transcription of tunicamycin-resistance and neighboring genes in a DNA region suitable for construction of a constitutive expression vector specific to Leishmania. Leishmania Molecular Biology Symp.14th Int. Cogr. Trop. Med. p. 88.

84. Chang, K.-P., Chai, J.J., Zuo, X.-P., Qu, J.Q., Sundar, S., Singh, S. and Reed, S.G. (1996) RK39 distick vs.

conventional procedures for diagnosis of Chinese and Indian visceral leishmaniasis. 14th Int. Cogr. Trop. Med. P. 238.

85. Chang, K.-P. (1996) Leishmania determinants of virulence and genetic heterogeneity: Strategies of control

for leishmaniasis. 14th Int. Cogr. Trop. Med. P. 30. 86. Akman, L., Lu, H.G., Wang, R.Q., Chang, K.-P., Aksu, H.A.Z., Culha, G., Ozcan, K., Ozbel, Y., Ozensoy,

S., Turgay, N., Alkan, Z. and Ozcel, M.A. (1996) Grouping of Turkish Leishmania isolates by nuclear and kinetoplast DNA polymorphisms. 14th Int. Cogr. Trop. Med. P. 91.

87. Chai, J.J., Zuo, X.-P., Lei, Y., Hou, Y.Y., Ruziguli, Zhang, S. and Chang, K.-P. (1996) Preliminary

vaccination trials against visceral leishmaniasis in rodent model indigenous to northwestern regions of China. 14th Int. Cogr. Trop. Med. P. 359.

88. Chang, K.-P., Lu, H.G., McGwire, B.S. and Kawazu, S.-I. (1997) Functional significance of Leishmania

gene encoding N-acetylglucosamine-1-phosphate transferase. WorldLeish I. p. 72. 89. Lu, H.G., Zhong, L., Chang, K.-P. and Docampo, R. (1997) Calcium and cell invasion in Leishmania

amazonensis. WorldLeish I. p. 74. 90. Chang, K.-P., Chai, J.J., Zuo, X.P., Qu, J.Q., Kawazu, S.-I., Katakura, K. and Matsumoto, Y., and Reed,

S.G. (1997) RK39 dipstick vs. conventional procedures for diagnosis of visceral leishmaniasis in northwestern China. WorldLeish I. p.127.

91. Katakura, K., Kawazu, S.-I., Ito, M., Naya, T., Pang, H., Nagakura, K., Aikawa, M., Qu, J.-Q., Guan, L.-R.,

Chai, J.-J., Chang, K.-P. & Matsumoto, Y. (1997) PCR diagnosis of kala-azar patients in Xinjiang, China. WorldLeish I. p. 128.

92. Ozensoy, S., Ozbel, Y., Alkan, M.Z., Turgay, N., Gul, K., Chang, K.-P., Reed, S.G. & Ozcel, M.A. (1997)

The current status of leishmaniasis in Turkey. WorldLeish I. p. 129. 93. Akman, L., Wang, R.Q., Aksu, H.A.Z., Culha, G., Ozcan, K., Ozbel, Y., Ozensoy, S., Turgay, N., Alkan,

Z., Ozcel, M.A. and Chang, K.-P. (1997) Grouping of Turkish Leishmania isolates by nuclear and kinetoplast DNA polymorphisms. WorldLeish I. p. 128.

94. Ozensoy, S., Akman, L., Ozbel, Y., Turgay, N., Korkmaz, M., Ozcel, M.A., Alkan, M.Z., Skeiky, Y., Reed,

S.G. & Chang, K.-P. (1997) Kinensin K39 repetitive epitope found by PCR and Southern analyses in Turkish isolates causing cutaneous and visceral leishmaniasis. WorldLeish I. p. 152.

95. Singh, S., Chang, K.-P. & Reed, S.G. (1997) Blood transfusion transmitted kala-azar: Indian Scene.

WorldLeish I. p. 158.

52


PUBLICATIONS - Abstracts: (Con’t) 96. H.-G. Lu, S.-I. Kawazu, H. Ito, D.-Q. Chen, M. Fakruddin, L. Akman, N. Yadava and K.-P. Chang (1998)

Replacement of P36 with foreign genes for their chromosomal and extrachromosomal expression in Leishmania amazonensis. Parasitol Internation. 47 (Suppl.): 219

97. J.-Q. Qu, L.R. guan, I. Shulidan, X.-P. Zuo, J.-J. Chai, S.-B. Chen, D.-K. Wang, S.-I. Kawazu, K. Katakura, Y.

Matsumoto, S.G. Reed and K.-P. Chang (1998) Rapid serodiagnosis with rk39 dipsticks for viscerla leishmaniasis of low endemicity in northwestern China. Parasitol Internation. 47 (Suppl.): 227.

98. Ozensoy, S., Y. Ozbel, N. Turgay, M.Z. Alkan, M.A. Ozcel, K. Gul, S.G. Reed, A. Gilman-Sachs and K.-P.

Chang (1998) Recombinant K39 antigen for sero-diagnosis and -epidemiology of human and canine visceral leishmaniasis in western Turkey. Parasitol Internation. 47 (Suppl.): 227.

99. Chang, K.-P., J. F. Sah, H. Ito, B. K. Kolli and S. Sassa (2000) Defects of trypanosomatid protozoa in heme

biosynthesis: Porphyria of xenotransgenic Leishmania with mammalian heme pathway enzymes. Mill. Meet. Porphyrins Porphyrias 2000 P. 45.

100. Chang, K.-P. (2001) A “leishmaniac” manifesto. WorldLeish II P. 1. 101. Aguiar F., Cupolillo E., Chang K-P., Kemp S.J., Noyes H.A. (2001) Comparative evolution of the N-

glycosylation associated N-acetylglucosamine-1-phosphate transferase gene and the houskeeping 6-phosphogluconate dehydrogenase gene. WorldLeish II P. 46

102. Culha, G. & Chang, K.-P. (2001) Conventional and PCR methods for diagnosis of cutaneous leishmaniasis in

Cukurova region of Turkey. WorldLeish II P. 115. 103. Hajmova M., Volf P., Kolli B. K. & Chang, K.-P. (2001) Gp63 expression of Leishmania amazonensis affects

their early infection in the midgut of Lutzomyia longipalpis. WorldLeish II P. 124. 104. Hwa K.Y., Khoo K.H., Chang, K.-P. (2001) Correlation between pathological spectra of leishmaniasis and

parasite glycoconjugate structures. WorldLeish II P. 29. 105. Kawazu S.-I., Katakura K., Ozbel Y., Nagakura K., Hatabu T., Ito M., Nakai Y.,Qu J.-Q., Aikawa M., Chang

K.-P., Kamio T., Kano S., and Matsumoto Y. (2001) Differentiation of Leishmania species complexes based on PCR amplification of a highly conserved gene (p36). WorldLeish II P. 48

106. Kolli, B. K., McGwire, B. S., Mottram, J. C and Chang, K.-P. (2001) Release of gp63 and diminished in vitro

infectivity of a GPI -lacking null mutant from Leishmania mexicana. WorldLeish II P. 26. 107. Lu H.G., Chen D.-Q., Kolli B.K.and Chang K.-P. (2001) Replacement of two highly conserved neighboring

genes in Leishmania amazonensis: Lethal single knockout of N-acetylglucosamine-1-phosphate transferase and lack of effect for double knockout of NADPH-oxidoreductase (p36). WorldLeish II P.64.

108. Matsumoto Yasunobu, Hatabu T., Ito M., Sanjoba C., Chang K.-P.& Kawazu S. (2001) Role of

polymorphonuclear leucocytes in cutaneous leishmaniasis. WorldLeish II P.36. 109. Matsumoto Yoshitsugu, Terabe M., Kuramochi T., Ito M., Hatabu T., Sanjoba C., Matsumoto Yasunobu,

Kawazu S., Ondera T., and Chang K.-P (2001) CD4+ cells are indispensable for ulcer development in murine cutaneous leishmaniasis. WorldLeish II P. 8.

110. McGwire, B. S., Chang, K.-P. and Engman, D. M. (2001) Cell Surface Autoproteolytic and secretory release of

leishmanial Ecto-Metalloprotease (gp63): Evidence of a Conserved, Virulence-associated Phenotype. WorldLeish II P. 26.

53


PUBLICATIONS - Abstracts: (Con’t) 111. Özensoy Töz, S. & Chang, K.-P. (2001) rK39 dipstick for diagnosis of visceral leishmaniasis in Turkey.

WorldLeish II P. 58. 112. Qu J.-Q., Guan L.-R., Chen S.-B., Shulida I., Kawazu. S., Katakura K., Matsumoto Y., Reed S. G. and Chang,

K.-P. (2001) rK39 dipstick rapid diagnosis for visceral leishmaniasis of Low endemicity. WorldLeish II P. 116. 113. Singh N., Schneider J., Ramsing Th., Rastogi A.K., Sundar S., & Chang K.-P. (2001) DNA homology of

leishmania donovani isolates from Indian patients of kala azar sensitive and refractory to chemotherapy. Is PG1 relevant to antimonial resistance ? WorldLeish II P.99.

114. Svobodová M., Chang K.-P., Volf P. (2001) Laboratory evidence suggestive of Phlebotomus sergenti–rat

transmission cycle in the endemic focus of cutaneous leishmaniasis due to Leishmania tropica in Sanliurfa, Turkey. WorldLeish II P. 51.

115. Thiakaki M., Konidou G., Chang K.-P. and Soteriadou K. (2001) Modulation of gp63 level in promastigotes

affects the outcome of Leishmania amazonensis infection in BALB/c mice. WorldLeish II P. 100. 116. Kolli, B.K., Kostal, J., Zaborina, A.M., Chakraborty, A.M. and Chang, K.-P. (2002) Leishmania model for

microbial virulence: Secretion of nucleoside diphosphate kinase prevents apoptosis of the host macrophages. 5th Ann. Meet. New & Re-emerging Dis., Urbana-Champaign University of Illinois, P. 25-26.

117. Waki, K., Nordstrom, M., Akman, L., Tdymir, R., Mukhtar, M.M., Schallig, H., Chulha, G., Singh, N. &

Chang, K.-P. (2002) N-acetylglucosmine-1-phosphate transferase gene as a potential marker for genotyping Old World Leishmania isolates. 5th Ann. Meet. New & Re-emerging Dis., Urbana-Champaign University of Illinois, P. 25.

118. Waki K, Akman L, Toydemir R, Schallig H, Mukhtar M, Chai JJ, Guan LR, Culha G, Ozensoy S, Ozbel Y,

Singh N, Saliba EK, Krasnonos L., Nahhas S, Chang KP (2003) Separation of ~100 Old World Leishmania isolates into fourteen genotypes of N-acetylgluocsamine-1-phosphate transferase, Ann Meet Am Soc Trop Med Hyg.

119. Dutta, S., Kolli, B.K., Chang, K.-P. (2003) Therapeutic and prophylactic potentials of Leishmania forward and

reverse genetic mutants in heme biosynthesis pathway. Ann Meet Am Soc Trop Med Hyg. 120. Dutta S, Kolli BK and Chang KP 2004. Forward and Reverse Genetic Mutants in the Heme Biosynthesis

Pathway of Leishmania. Chicago Area Mol. Parasitol. and Mycol. Poster. 121. Ray D., Kolli B.K., Chang, K.-P. 2004. Phototoxicity of Al-phthalocyanine against Leishmania amastigotes and

promastigotes. Chicago Area Mol. Parasitol. and Mycol. Poster. 122. Tang, A.H., Dutta, S., Kolli, B.K., Waki, K., Sassa, S., Chang, K.-P. 2004. Stability of transgenes responsible

for ALA-induced uroporphyria of Leishmania engineered as suicidal mutants. Chicago Area Mol. Parasitol. and Mycol. Poster

123. Kolli, B.K., Chang, C., Showe, L.C., Showe, M., Chang, K.-P. 2004. Expression profiles of macrophages and

their intracellular parasites in infected cultures predict vaccinability of oprphyric suicidal Leishmania. Chicago Area Mol. Parasitol. and Mycol. Poster.

124. Chang, K.-P., et al. 2004. In vivo pathogenicity and immunogenicity of light-sensitive Leishmania suicidal

mutants transgenetically engineered to develop poprhyria: Preliminary appraisals for their potential application as a universal platform for vaccination. Chicago Area Mol. Parasitol. and Mycol. Poster.

54


PUBLICATIONS - Abstracts: (Con’t) 125. Waki, K., Chang. K.-P. et al. 2004. Virulence genes of repetitive A2 and K39 present in both cutanotropic and

visceralizing Leishmania infantum. Chicago Area Mol. Parasitol. and Mycol. Poster. 126. Chang, K.-P. et. al. 2005. Porphyric Leishmania as inducible suicidal mutants for photodynamic vaccination.

Chicago Area Mol. Parasitol. and Mycol. Poster. 127. Dutta S, Kolli BK and Chang KP 2005. Genetic Mutants in the Heme Biosynthesis Pathway of Leishmania.

Chicago Area Mol. Parasitol. and Mycol. Poster. 128. Waki, K., Dutta, S., Ray, D., Kolli, B. K. and Chang, K.-P. 2005 Sequence bioinformatics of Leishmania

single-copy genes and repetitive sequences in relation to phylogenetic genotypes and disease phenotypes. Chicago Area Mol. Parasitol. and Mycol. Poster.

129. Kolli, B.K., Chang, C., Showe, L.C., Showe, M. and Chang, K.-P.2005. Expression profiles of macrophages

and their intracellular parasites in infected cultures predict safety and vaccinability of porphyric suicidal Leishmania. Chicago Area Mol. Parasitol. and Mycol. Poster.

130. Kolli, B.K., Kostal, J., Zaborina, O, Chakraborty, A.M. and Chang, K.-P. 2005.Intracellular Leishmania release

molecules to prevent apoptosis of macrophages. Chicago Area Mol. Parasitol. and Mycol. Poster. 131. Kuo-yuan Hwa, Kay-hooi Khoo, Chan-Wei Chen and Kwang-Poo Chang 2006. Structural correlation of

glycoinositol phospholipids and pathological spectra of leishmaniasis from the Old World parasite isolates FASEB J., March 20 (Meeting Abstract Supplement) LB57.

132. Chang, K.P., Kolli, B.K., Waki, K., Dutta, S., Folkova, D. and Volf, P. 2006. Let the evils fight the devisls:

Parasites against infectious diseases and cancer ? 4th Med. Congr. Pharm., Med. J. Sci. Tech. 2, P. 26. 133. Chang, KP, Kolli, B., Dutta, S. (2007).Leishmaniac jihad, Int Conf Env Parasit/Commun Heal Initiativ., Aug

Found Dayalbagh Educational Institute. Proceeding book. 134. Chang, KP, Waki, K., Kolli, B, Dutta, S. (2009) Ambiguity of multiple DNA versus single-copy protein coding

genes for Leishmania genotyping. # 10, Feb. 2, “WorldLeish 4”, Lucknow, India. 135. Chang, KP, Kolli, B, Dutta, S. (2009) Photodynamic vaccination with porphyric suicidal mutants against

leishmaniasis, Feb. 4, #. 45, “WorldLeish 4”, Lucknow, India 136. Kolli, B., Dutta, S., Chang, KP (2009) Leishmania releases nucleoside diphosphate kinase to prevent apoptosis

of macrophages. Feb. 8, #323, “WorldLeish 4”, Lucknow, India 137. Chang, KP, Dutta, S., Kolli, B (2009) Immunity to Leishmaniasis and Vaccination”, International Symposium

on Leishmaniasis Vaccines, # 25, Mar. 9-11, Recife, Brazil 138. Chang, KP (2009) Elucidation of parasitic mechanisms from laboratory to clinico-epidemiologically relevant

Leishmania for developing effective prophylactic and therapeutic strategies”, US-NIH-NIAID/Inst Pasteur Tunis Joint Conference “Leishmania: Collaborative Research Opportunities in North Africa and the Middle East”, May 5-9, Tunis, Tunisia.

139. Chang, KP (2009) Genetic and chemical engineering of Leishmania for photodynamic vaccination, XIV

Colombia Cong Parasitol/Trop Med., Medellin, Colombia. 140. Mehta, SJ, , Huang R, Yang M, Zhang XQ, Kolli B, Chang KP, Hoffman, Schooley RT. (2010) Evaluation of

SLC11A1 role in host response to Leishmania amazonensis in vitro and in vivo. P. 40, #25, Ann Meet Soc Trop Med Hyg, Nov. 4, Atlanta, GA.

55


PUBLICATIONS - Abstracts: (Con’t) 141. Chang, KP (2012) Photodynamically inactivated Leishmania as a potential novel carrier for vaccine delivery

against infectious and non-infectious diseases. #. 22, 2nd Int Conference Vaccine/Vaccination, Aug. 20-22, Northbrook, IL

142. Chang, KP (2012) Toward automation of Leishmania photodynamic vaccination. 2nd Int. Symp. Leishmaniasis

vaccines. Sep. 2-7, Ouro Preto, Brazil. 143. Chang, KP (2013) Photo-inactivation of Leishmania with ROS for safe and effective delivery of vaccines

against infectious and malignant diseases, P. 6, # 17, Mar. 19, BIT World Vaccine Conference, Hanzhou, China.

144. Shio, MT, Jung JY, Chang, KP, Olivier M. (2013) Impact of Leishmania Metalloprotease GP63 on NLRP3

Inflammasome Complex. #1243. May 13-18, Worldleish 5, Recife, Brazil. 145. Chang, KP (2013) A call for long-term investigation of all endemic sites to elucidate global clinico-

epidemiology of leishmaniasis: a multidisciplinary consortium is required to complete the database and to archive clinical samples for 'leishmaniome’ analysis. #1370. May 13-18, Worldleish 5, Recife, Brazil.

146. Chang, KP (2013) Photoinactivation of leishmania with ROS provides a safe and effective vaccine/vaccine

carrier for photodynamic vaccination. #1319. May 13-18, Worldleish 5, Recife, Brazil. 147. Mishra, AK, Singh SP, Kolli, BK, Bansal, M, Chang, KP and J Pratap, JV(2013) Cloning, overexpression,

purification, crystallization and data processing of Nucleoside Diphosphatase Kinase (NDK) from Leishmania amazonensis.#109, May 13-18, Worldleish 5, Recife, Brazil.

148. Linchangco, R, Tripathi, P, Chang KP (2013) Additional transfection of porphyrinogenic Leishmania with ALA

synthase for automatic production of uroporphyrin. Oct. 20, CMS Summer Fellowship Poster Session, North Chicago, IL.

149. Chang KP, Kolli BK, Tripathi P, Ng DKP, Chang CS, Alkahtani S, Al-Qahtani A, de Oliveira CI, Mello S,

Manna L, Head JF, Elliott RL, Batchu RB (2014). Photodynamic vaccination: Novel strategies of vaccine delivery by intraphagolysosomal parasites and their oxidative inactivation for safe and effective applications, Nov. 17. 2nd Int Congr Bacteriol Inf Dis, Chicago, IL, USA.

150. Andrew Surro, Pankaj Tripathi, KP Chang (2014)“Additional Transfection of Porphyrinogenic Leishmania

with ALA Synthase for Automatic Production of Uroporphyrin”, Summer Student Poster Session, Oct. 31, Chicago Medical School/RFUMS, USA

151. B. Perea, A. Tikoo, A. Gilman Sachs, B. Kolli, KP. Chang (2014) “Photodynamic vaccination in vitro:

Inactivation of GFP fluorescent Leishmania with Human Peripheral Phagocytes”, Summer Student Poster Session, October 31. Chicago Medical School/RFUMS, USA

152. Saad Alqhatani, Ahmed Al-Qhatani, Dennis K. P. Ng, Chin Shen Chang, Kayoko Waki, Pankaj Tripathi, Bala

Kolli, Kwang Poo Chang (2014) “Endosome-targeting photodynamic therapy of cutaneous leishmaniasis due to Leishmania tropica” Int J Art Sci Conf, October. *Presenter, Boston, USA

153. BK Kolli, JP Cruzin, G Ramasamy, PM Myler and KP Chang (2015) “Next-generation sequence analysis for

differential gene expression between promastigote and amastigote stages of Leishmania amazonensis”, All Sch Res Conf. , March 18. . Chicago Medical School/RFUMS, USA

154. S Al-Qahtani, A Al-Qahtani, BK Kolli, P Tripathi, DKP Ng, KP Chang (2015) “Oxidative photo-inactivation

of Leishmania with polyamine-phthalocyanine conjugates for vaccination”, ”, All Sch Res Conf., March 18, . Chicago Medical School/RFUMS, USA

56

155. Zurek A, Chang KP, Kolli BK (2015) Leishmania: Threat and Opportunity – Phylogenetic Identification of the

Parasite Species through DNA Sequencing. DePaul/CMS Student Research Poster Session, October 23, Chicago, IL, USA.

156. Chang KP, Kolli BK (1015) Novel strategy for safe and effective vaccine delivery to elicit cell-mediated

immunity against infectious and malignant diseases. December 2, Vaccine R&D-2015, San Francisco, CA, USA.

157. Chang KP, Kolli BK (1016) New “light” for one-world approach toward safe and effective control of animal

diseases and insect vectors. 11th CVBD Forold Forum, May 9-11, Miami, FL., USA

158. Chang KP (1016) Visceral leishmaniasis in south and east Asia. 11th CVBD Forold Forum, May 9-11, Miami, FL, USA

159. Chang KP (1016) Tarim hare as a potential reservoir for desert-type visceral leishmaniasis in Xinjinag, China .

11th CVBD Forold Forum, May 9-11, Miami, FL, USA 160. Ozbel Y, Sanjoba C, Sun JM, Karakus M, Chang KP, Tsai CW, Matsumoto Y (2016) An Entomological Survey

for Sand flies in Two Counties of Taiwan. Int Symp Phlebotomine Sandflies. Jun 28 to July 1. Reims, France.

161. Kwang Poo Chang (2016) Photodynamically inactivated Leishmania for safe and effective delivery of vaccines for immunotherapy of infectious and malignant diseases. Int Conf Human Papillomavirus. October 20, 2016 Chicago, USA.

162. Chang KP, Kolli BK, Jiang XP, Elliott RL (2017) Photodynamic vaccination: Light inactivation of photo-sensitized Leishmania with singlet oxygen as safe and effective vaccines and vaccine carriers for immuno-prophylaxis and –therapy. WorldLeish 6, May 16-20, Toledo, Spain.

163. Sharma R, Viana SM, Kolli BK, Chang, KP de Oliveira CI (2017). Delta-aminolevulinate-inducible uroporphyrinogensis in transgenic Leishmania braziliensis; a tool for photodynamic vaccination WorldLeish 6, May 16-20, Toledo, Spain.

164. Viana SM, Celes FS, Laura Ramirez G., Kolli BK, Ng DKP, Chang KP, de Oliveira CI (2017). Photo-inactivation of Leishmania amazonensis as an immunoprophylaxis tool for Cutaneous Leishmaniasis. Worldleish 6, May 16-20, Toledo, Spain.

165. Manna IL, Corso R, Kolli BK, Chang KP (2017). Immunotherapy of canine leishmaniasis by photodynamic vaccination. WorldLeish6, May 16-20, Toledo, Spain.

166. Chang KP, Kolli BK, Ng DKP, Shiao SH, Manna L, Elliott RL, Fu GL (2018) Screening novel phthalocyanines for developing photodynamic-vaccination and –insecticides, SPIE- Int Soc Optic & Photonic, Feb. 26 – Mar 1, San Francisco.

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