cytology the structure of the cell at light and …...cytology the structure of the cell at light...
TRANSCRIPT
![Page 1: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/1.jpg)
CYTOLOGY the structure of the CELL at light
and electron-microscopic level
Institute of Histology and EmbryologyFaculty of Medicine
Comenius University
Practical training N.2
![Page 2: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/2.jpg)
SPECIMENS
• DNA - the nuclei of the epithelial cells,
Vagina (IH-E)
• RNA – ribosomes, rough ER, nucleolus,
Spinal cord (HE)
• Mitochondria, Kidney (Regaud)
• Glycogen, Liver of rat (PAS or PAS-H)
• Pigment granules, pigmentary epithelium of
Retina (without staining)
• Brush border, microvilli – Kidney (AP)
![Page 3: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/3.jpg)
Specimen:
DNA - the nuclei of the
epithelial cells,
Vagina
Iron hematoxylin and Eosin
![Page 4: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/4.jpg)
Various types of nuclei in the cells
of stratified squamous epithelium
nonkeratinized
![Page 5: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/5.jpg)
DNA – nuclei of the epithelial cells, Vagina (IH-E)
![Page 6: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/6.jpg)
DNA – nuclei of the epithelila cells, Vagina (IH)
![Page 7: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/7.jpg)
Specimen:
RNA – ribosomes, rough ER,
nucleolus, Spinal cord
hematoxylin and eosin
![Page 8: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/8.jpg)
![Page 9: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/9.jpg)
Nissl substance (tigroid) – rER in neuron
![Page 10: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/10.jpg)
Spinal
cord
![Page 11: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/11.jpg)
Anterior horn
RNA – ribosomes, rough ER, nucleolus, Spinal cord (HE)
![Page 12: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/12.jpg)
![Page 13: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/13.jpg)
![Page 14: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/14.jpg)
![Page 15: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/15.jpg)
![Page 16: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/16.jpg)
![Page 17: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/17.jpg)
Specimen:
Demonstration of mitochondria
in the cells of proximal tubules,
Kidney
Regaud
![Page 18: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/18.jpg)
The cell of proximal tubulein kidney,mitochondria
![Page 19: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/19.jpg)
![Page 20: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/20.jpg)
Mitochondria, kidney (Regaud)
![Page 21: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/21.jpg)
Mitochondria, kidney (Regaud)
erytrocytes
mitochondria
lumen
of tubule
![Page 22: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/22.jpg)
Mitochondria, kidney (Regaud)
![Page 23: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/23.jpg)
Specimen:
Demonstration of glycogen
in hepatocytes, Liver
PAS–H
![Page 24: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/24.jpg)
![Page 25: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/25.jpg)
Glycogen
particules
in hepatocyte
at EM level
![Page 26: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/26.jpg)
Glycogen - Liver (PAS-H)
![Page 27: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/27.jpg)
Glycogen - Liver (PAS-H)
nucleus
![Page 28: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/28.jpg)
Glycogen - Liver (PAS-H)
nuclei
glycogen
![Page 29: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/29.jpg)
Glycogen - Liver (PAS-H)
glycogen
![Page 30: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/30.jpg)
Specimen:
Demonstration of endogenic
pigments –
pigmentary epithelium of Retina,
Eye ball
without any staining
![Page 31: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/31.jpg)
![Page 32: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/32.jpg)
Pigmentary epithelium, retina
![Page 33: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/33.jpg)
![Page 34: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/34.jpg)
Unstained nucleus
Pigment granules
![Page 35: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/35.jpg)
Specimen:
Demonstration of specialized
cellular surfaces: microvilli in the
lining epithelial cells of proximal
tubules, Kidney
Evidence of positivity (red) of alkaline
phosphatase bounded at the surface
of microvilli
![Page 36: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/36.jpg)
![Page 37: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/37.jpg)
Microvilli – kidney (AP)
![Page 38: CYTOLOGY the structure of the CELL at light and …...CYTOLOGY the structure of the CELL at light and electron-microscopic level Institute of Histology and Embryology Specimen: Demonstration](https://reader036.vdocuments.net/reader036/viewer/2022071019/5fd35843ada30b4b1f53b664/html5/thumbnails/38.jpg)