devils lake, sheyenne river, red river and lake winnipeg

11

Devils Lake, Sheyenne River, Red River and Lake Winnipeg

Summary of Results

Parasite and Pathogen Monitoring

IRRB Aquatic Ecosystem CommitteeDavid Donald, Co-Chair, CanadaRick Nelson, Co-Chair, U.S.

Presented by:

22

PROGRAM FOCUSPROGRAM FOCUSU.S. and CanadaU.S. and Canada

PARASITOLOGYPARASITOLOGY

HISTOPATHOLOGY HISTOPATHOLOGY Note: Devils Lake only in U.S. Note: Devils Lake only in U.S.

BACTERIOLOLGYBACTERIOLOLGY

VIROLOGYVIROLOGY

33

1. Determine the presence/absence of fish parasites and pathogens in resident fish from Devils Lake, Sheyenne River, Red River, Red River Delta, and Lake Winnipeg;

2. Provide a comprehensive, and scientifically credible survey of fish parasites and pathogens in fish from Devils Lake, Sheyenne River, Red River and Red River Delta. The data can be used in performing risk analyses associated with transfer of fish parasites and pathogens from the outlet on Devils Lake to aquatic ecosystems in the Red River basin including Lake Winnipeg.

3. Use the comprehensive survey of fish collected during this proposed survey to meet the overall framework for biological monitoring in the Red River basin that is included in the “Work Plan” of the International Red River Board.

Program Objectives

44

Background

February 2006 – AEC submits proposal for funding to IJC

August 2006 – 1st Joint US-Canada Technical Workshop- design sampling program and agree on methods

March 2007 – 2nd Joint US-Canada Technical Workshop- review 2006 data; make agreed upon changes to program;discuss risk analysis with experts

March 2008 – 3rd Joint US-Canada Technical Workshop- review 2007 data; make agreed upon changes to program

55

DL sub-basins covers ~2.4 million acres (~3,800 sq. miles).DL sub-basins covers ~2.4 million acres (~3,800 sq. miles). Devils Lake sub-basin (yellow) located within Hudson Bay Devils Lake sub-basin (yellow) located within Hudson Bay

Drainage (pink).Drainage (pink).

66

FISH PARASITESAND

PATHOGENS

U.S.

Rick Nelson, Co-Chair, United StatesIRRB Aquatic Ecosystem

77

Targeted SpeciesTargeted SpeciesSpeciesSpecies Devils LakeDevils Lake Red BasinRed Basin

Canada + USACanada + USA

Northern PikeNorthern Pike ++ ++

WalleyeWalleye ++ ++

White bassWhite bass ++ ++

Brook SticklebackBrook Stickleback ++ ++

Fathead minnowFathead minnow ++ ++

Yellow perchYellow perch ++ ++

GoldeyeGoldeye ++

Channel catfishChannel catfish ++

SaugerSauger ++

Emerald shinerEmerald shiner ++

88

TARGETED PATHOGENS:TARGETED PATHOGENS:

Virology: Virology: VHS, IPN, IHN, VHS, IPN, IHN,

Bacteriology: Bacteriology: Yersinia ruckeriYersinia ruckeriEdwardsiella ictaulriEdwardsiella ictaulriRenibacterium salmoninarumRenibacterium salmoninarumAeromonas salmonicidaAeromonas salmonicida

Parasitology:Parasitology:Comprehensive analysis – On-site mobile laboratory – U.SComprehensive analysis – On-site mobile laboratory – U.SPreserved samples for laboratory analysisPreserved samples for laboratory analysis

Histopathology:Histopathology:Comprehensive parasite analysisComprehensive parasite analysis

U.S -CanadaU.S -Canada

TARGETED PATHOGENSTARGETED PATHOGENS

1010

2006-2007 PARASITOLOGY2006-2007 PARASITOLOGYPARASITESPARASITES DEVILS LAKEDEVILS LAKE

20062006

624 fish624 fish

9 parasites9 parasites

DEVILS LAKEDEVILS LAKE

20072007

407 fish407 fish


RED RIVER RED RIVER BASIN USA-2006BASIN USA-2006

Sheyenne and Sheyenne and Red River-Red River-

150 fish and 3 150 fish and 3 parasitesparasites


Includes Lake Includes Lake Traverse-674 Traverse-674

Fish 34 Fish 34 parasitesparasites

Apiosoma sp.Apiosoma sp. ++ ++

AchtheresAchtheres sp sp ++

TrichodinaTrichodina sp sp ++ ++ ++

CleidodiscusCleidodiscus sp. sp. ++

CamallanusCamallanus sp sp ++

CorallobrothriumCorallobrothrium sp sp ++

Dactylogyrus sp.Dactylogyrus sp. ++ ++

Diplostomum spathaceumDiplostomum spathaceum ++

Epistylus sp.Epistylus sp. ++ ++

ErgasilusErgasilus sp. sp. ++

Gyrodactylus hoffmaniGyrodactylus hoffmani ++ ++ ++

Ichthyobodo necatorIchthyobodo necator ++

Piscicola punctataPiscicola punctata ++ ++

Paurorhynchus hiodontisPaurorhynchus hiodontis ++

Pomphorhynchus bulbocolliPomphorhynchus bulbocolli ++

1111

PARASITES-ContinuedPARASITES-Continued DEVILS LAKEDEVILS LAKE

20062006

624 fish624 fish



20072007

407 fish407 fish


RED RIVER RED RIVER BASIN USA-BASIN USA-

20062006




20072007



AncyrocephalidaeAncyrocephalidae ++

Posthodiplostomum Posthodiplostomum sp.sp. ++ ++

Myzobdella lugubrisMyzobdella lugubris ++ ++

Neascus sp.Neascus sp. ++ ++

Onchocleidus chrysopsOnchocleidus chrysops ++

Bothriocephalus cuspidatusBothriocephalus cuspidatus ++ ++ ++

Proteocephalus pinguisProteocephalus pinguis ++ ++ ++

HexamitaHexamita sp. sp. ++

HenneguyaHenneguya sp sp ++

Ligula intestinalisLigula intestinalis ++

Microcotyle spMicrocotyle sp ++

MyxobolusMyxobolus sp sp ++

Raphidascaris acusRaphidascaris acus ++ ++

SpinitectusSpinitectus sp. sp. ++

SpiroxysSpiroxys sp. sp. ++

Contracecum Contracecum sppspp ++ ++ ++ ++

1212

PARASITES-cont.PARASITES-cont. DEVILS LAKEDEVILS LAKE

20062006

624 fish624 fish



20072007

407 fish407 fish








Pleistophora ovariaePleistophora ovariae ++

Unicauda sp.Unicauda sp. ++

Clinostomum marginatumClinostomum marginatum ++

Ligictaluridus sp.Ligictaluridus sp. ++

Hunterella nodulosaHunterella nodulosa ++

Khawia iowensisKhawia iowensis ++

Valipora sp.Valipora sp. ++

Rhabdochona sp.Rhabdochona sp. ++

1313

Total Species, and Change in Species Total Species, and Change in Species Composition between years for Fish Parasites Composition between years for Fish Parasites

in Devils Lakein Devils Lake

0

2

4

6

8

10

12

2005 2006 2007

Change in speciescomposition betweenyears

Total species

1414

Collected 6 parasites at Devils Lake not previously observed Collected 6 parasites at Devils Lake not previously observed in surveys from 2005 and 2006.in surveys from 2005 and 2006.– References from Red River basin for most.References from Red River basin for most.– Onchocleidus chrysopsOnchocleidus chrysops commonly reported from gills of commonly reported from gills of

centrarchids, family is wide-spread across N.A. Also centrarchids, family is wide-spread across N.A. Also detected in Canada in 2007.detected in Canada in 2007.

Parasitofauna of fish in Lake Traverse appears more diverse Parasitofauna of fish in Lake Traverse appears more diverse compared to Devils Lake.compared to Devils Lake.

Gyrodactylus hoffmani Gyrodactylus hoffmani confirmed in fathead minnows from confirmed in fathead minnows from Lake Traverse. Also detected in Canada in 2007.Lake Traverse. Also detected in Canada in 2007.

Bothriocephalus acheilognathi (Asian tapeworm) not detected Bothriocephalus acheilognathi (Asian tapeworm) not detected in USin US

2007 Parasite Summary2007 Parasite Summary

1515

BacteriumBacterium 20052005

313 fish-5313 fish-5

bacterialbacterial

isolatesisolates

20062006

383 fish-7 383 fish-7 bacterial bacterial isolatesisolates

20072007

289 fish – 3 289 fish – 3 bacterial bacterial isolatesisolates

20082008


Acinetobacter lwoffiiAcinetobacter lwoffii ++

Aeromonas hydrophila Aeromonas hydrophila ++ ++ + + ++

Brevundimonas diminutaBrevundimonas diminuta ++

Corynebacterium renaleCorynebacterium renale ++

Hafnia alveiHafnia alvei ++ ++ ++

Pleisomonas shigelloidesPleisomonas shigelloides ++

Pseudomonas fluorescens Pseudomonas fluorescens ++

Pseudomonas Pseudomonas spsp ++

Pseudomonas mendocinaPseudomonas mendocina ++

Shewanella putrifaciensShewanella putrifaciens ++ ++

Streptococcus sobrinsStreptococcus sobrins ++

Yokenella regensburgiiYokenella regensburgii ++

BacteriologyDevils Lake

1616

Change in Species Composition Change in Species Composition of Bacteria Speciesof Bacteria Species

Devils LakeDevils Lake

0

1

2

3

4

5

6

2005 2006 2007 2008

1717

BacteriumBacterium

20062006


20072007

488 fish – 7 488 fish – 7 bacterial isolatesbacterial isolates

20082008

Ashtabula Ashtabula pending- Red pending- Red

River not River not sampledsampled

Aeromonas hydrophilaAeromonas hydrophila ++ + +

Citrobacter sp.Citrobacter sp. ++

Enterobacter cloacaeEnterobacter cloacae ++

Hafnia alveiHafnia alvei ++ ++

Pantoea sp.Pantoea sp. ++

Pasturella sp.Pasturella sp. ++

Plesiomonas sp.Plesiomonas sp. ++

Pseudomonas fluorescensPseudomonas fluorescens ++

Shigella sp.Shigella sp. ++

Stenotrophomonas sp.Stenotrophomonas sp. ++

BacteriologySheyenne and Red Rivers

1818

BacteriumBacterium 20072007


20082008

534 fish-2 534 fish-2 bacterial isolatesbacterial isolates

Erwinia sp.Erwinia sp. ++

Enterobacter sp.Enterobacter sp. ++

Edwardsiella tardaEdwardsiella tarda ++

ChlamydialesChlamydiales ++

Hafnia alveiHafnia alvei ++

Pantoea sp.Pantoea sp. ++

Pseudomonas fluorescensPseudomonas fluorescens ++

Stenotrophomonas spStenotrophomonas sp ++

Yersinia ruckeriYersinia ruckeri ++

BacteriologyLake Traverse

1919

2006-2008 BACTERIOLOGY2006-2008 BACTERIOLOGY DISCUSSIONDISCUSSION

Yersinia ruckeriYersinia ruckeri detected in Lake Traverse (BLC)-2007- the only detected in Lake Traverse (BLC)-2007- the only listed pathogen detected. Extremely common bacterial listed pathogen detected. Extremely common bacterial organism found in multiple fish species. Was not associated organism found in multiple fish species. Was not associated with any clinical disease.with any clinical disease.

The additional bacterial isolates appear to be common for The additional bacterial isolates appear to be common for freshwater habitats and multiple aquatic species: freshwater habitats and multiple aquatic species: AeromonasAeromonas, , PsuedomonasPsuedomonas, and Enterics., and Enterics.

EpitheliocystisEpitheliocystis (chlamydia bacteria) seen histologically in (chlamydia bacteria) seen histologically in Black Crappie from Lake Traverse and Walleye in Devils Lake.Black Crappie from Lake Traverse and Walleye in Devils Lake.

Enteric bacteria may be considered opportunistic Enteric bacteria may be considered opportunistic environmental agents.environmental agents.

Clinical significance is uncertainClinical significance is uncertain

2020

Virology Summary- 2006-2008Virology Summary- 2006-2008

DEVILS LAKE: DEVILS LAKE: 1,465 FISH1,465 FISH

RED RIVER: RED RIVER: 547 FISH547 FISH

SHEYENNE RIVER: SHEYENNE RIVER: 449 FISH 449 FISH

LAKE TRAVERSE: LAKE TRAVERSE: 1,067 FISH1,067 FISH

LAKE ASHTABULA: LAKE ASHTABULA: 392 FISH392 FISH

Approximately 4000 fish sampled

2121

VIROLOGY 2006-2008VIROLOGY 2006-2008

SPECIAL NOTESPECIAL NOTE: : THE CELL CULTURE ASSAY THE CELL CULTURE ASSAY

SCREENS FOR VHSSCREENS FOR VHS: : Viral Hemorrhagic SepticemiaViral Hemorrhagic Septicemia

Clinical approach provides broad coverage of Clinical approach provides broad coverage of multiple species and potential replicating agentsmultiple species and potential replicating agents

No viral agents detectedNo viral agents detected

No clinical signs of viral infection or diseaseNo clinical signs of viral infection or disease

2222

Histology-2007Histology-2007

Similar community of parasites were found both macroscopically and microscopically in Similar community of parasites were found both macroscopically and microscopically in fish collected from Devils Lake and Lake Winnipeg. fish collected from Devils Lake and Lake Winnipeg.

Gill protozoans and cestodes in gastrointestinal tract were the most common findings in Gill protozoans and cestodes in gastrointestinal tract were the most common findings in Devils Lake.Devils Lake.

Myxosporeans detected in histology analysis and parasitology work.Myxosporeans detected in histology analysis and parasitology work.

Fewer parasites were found in fish from Devils Lake. Fewer parasites were found in fish from Devils Lake.

No significant pathology was associated with parasite infestation (external) or infection No significant pathology was associated with parasite infestation (external) or infection (internal).(internal).

Histology analysis was consistent with results from parasitology, bacteriology and Histology analysis was consistent with results from parasitology, bacteriology and virology.virology.

Histology reveals only a few microscopic parasites not observed with traditional methods.Histology reveals only a few microscopic parasites not observed with traditional methods.– Primarily myxosporean and early stages of protozoa.Primarily myxosporean and early stages of protozoa.– Speciation is difficultSpeciation is difficult

2323

Summary - 2007Summary - 2007

No unusual findings for bacterial agents for any No unusual findings for bacterial agents for any species in any locationspecies in any location

Bacterial results are not unusual for this type of Bacterial results are not unusual for this type of aquatic environmentaquatic environment

No detection of viral agents-very significantNo detection of viral agents-very significant

No indication of clinical disease resulting from No indication of clinical disease resulting from bacterial or viral agents for any fish species in any bacterial or viral agents for any fish species in any locationlocation

Additional parasites detected in Devils Lake and Additional parasites detected in Devils Lake and Lake Traverse Lake Traverse

2007 results for Devils Lake – no unusual findings – 2007 results for Devils Lake – no unusual findings – including parasitesincluding parasites

2424

2008 RED RIVER BASIN FISH HEALTH MONITORING2008 RED RIVER BASIN FISH HEALTH MONITORING SUMMARY OF SAMPLING June 8th-20th SUMMARY OF SAMPLING June 8th-20th

SAMPLE SITE #1: SAMPLE SITE #1: LAKE ASHTABULALAKE ASHTABULA11 Fish Species11 Fish SpeciesTotal Fish Sampled: 392Total Fish Sampled: 392

SAMPLE SITE #2: SAMPLE SITE #2: DEVILS LAKEDEVILS LAKE (Comprehensive Sampling Included Histology) (Comprehensive Sampling Included Histology)7 Fish Species7 Fish SpeciesTotal Fish Sampled: 296Total Fish Sampled: 296

SAMPLE SITE #3: SAMPLE SITE #3: LAKE TRAVERSELAKE TRAVERSE15 Fish Species15 Fish SpeciesTotal Fish Sampled: 534Total Fish Sampled: 534

SAMPLE SITE #4 SAMPLE SITE #4 RED RIVER AT FARGORED RIVER AT FARGO********* Sampling could not be completed due to flood stage water flows on the Red River: *** Sampling could not be completed due to flood stage water flows on the Red River: Normal Sampling Flow Conditions: 200 CFS. Red River Measured Flows at Sampling Time Normal Sampling Flow Conditions: 200 CFS. Red River Measured Flows at Sampling Time were in excess of 5,000 CFS. Hazardous for boat travel, electrofishing, and netting. No were in excess of 5,000 CFS. Hazardous for boat travel, electrofishing, and netting. No samples for spring season deadline June 21st.samples for spring season deadline June 21st.

GRAND TOTAL ALL FISH SAMPLED: 1,222GRAND TOTAL ALL FISH SAMPLED: 1,222** Additional samples were obtained for comprehensive parasitology screening at** Additional samples were obtained for comprehensive parasitology screening at all sitesall sites

PENDING: HISTOPATHOLOGY, PARASITOLOGYPENDING: HISTOPATHOLOGY, PARASITOLOGY

2525

United States – Red River Basin United States – Red River Basin Pathogen/Parasite SurveyPathogen/Parasite Survey

Rick Nelson (USBR), Co-Chair, U.S.Rick Nelson (USBR), Co-Chair, U.S.IRRB Aquatic Ecosystem CommitteeIRRB Aquatic Ecosystem Committee

Participating Projects:Participating Projects:USFWS Bozeman Fish Health CenterUSFWS Bozeman Fish Health CenterUSFWS LaCrosse Fish Health CenterUSFWS LaCrosse Fish Health CenterUSFWS Dexter Fish Health UnitUSFWS Dexter Fish Health UnitUSFWS Idaho Fish Health CenterUSFWS Idaho Fish Health CenterUSFWS Great Plains Fish and Wildlife Management Assist.USFWS Great Plains Fish and Wildlife Management Assist.USFWS Missouri River Fish and Wildlife Management Assist.USFWS Missouri River Fish and Wildlife Management Assist.USFWS Valley City National Fish HatcheryUSFWS Valley City National Fish HatcheryNorth Dakota Game and FishNorth Dakota Game and FishSouth Dakota Game, Fish and ParksSouth Dakota Game, Fish and ParksMinnesota DNR Division of Fish and WildlifeMinnesota DNR Division of Fish and Wildlife

Total Field Personnel: 24Total Field Personnel: 24

2626

USFWS-Bozeman Fish Health CenterUSFWS-Bozeman Fish Health CenterLaboratory Assays and ReportingLaboratory Assays and Reporting

VIROLOGY: VIROLOGY: Lacey Hopper, Amberly GallubLacey Hopper, Amberly Gallub

BACTERIOLOGY:BACTERIOLOGY: Molly Bensley, Tammy WeissMolly Bensley, Tammy Weiss

PARASITOLOGY: PARASITOLOGY: Linda Vannest, Tammy Weiss, Ken PetersLinda Vannest, Tammy Weiss, Ken Peters

HISTOPATHOLOGY: HISTOPATHOLOGY: Beth MacConnell, Charlie Smith,Beth MacConnell, Charlie Smith,

Tammy WeissTammy Weiss

REPORTING: REPORTING: Crystal Hudson, Ken Peters, Judy Kobus-FiskCrystal Hudson, Ken Peters, Judy Kobus-Fisk

Total Laboratory Personnel: 11Total Laboratory Personnel: 11

TOTAL SURVEY PERSONNEL: 35TOTAL SURVEY PERSONNEL: 35

2727

FISH PARASTIES FISH PARASTIES AND AND

PATHOGENSPATHOGENS

CANADACANADADavid Donald, Co-Chair, CanadaDavid Donald, Co-Chair, Canada

IRRB Aquatic Ecosystem CommitteeIRRB Aquatic Ecosystem Committee

2828

Targeted SpeciesTargeted Species SpeciesSpecies Devils LakeDevils Lake Red BasinRed Basin

Canada + USACanada + USA

Northern PikeNorthern Pike ++ ++

WalleyeWalleye ++ ++

White bassWhite bass ++ ++

Brook SticklebackBrook Stickleback ++ ++

Fathead minnowFathead minnow ++ ++

Yellow perchYellow perch ++ ++

GoldeyeGoldeye ++

Channel catfishChannel catfish ++

SaugerSauger ++

Emerald shinerEmerald shiner ++

2929

Map of ManitobaMap of Manitoba

3030

Sampling LocationsSampling Locations

White BassWhite Bass

(sampling locations (sampling locations 2006)2006)

3131

PARASITES 2006 & 2007PARASITES 2006 & 2007•Ten fish species, 30 individuals of each collected during September October 2006 (Total 300 fish)

•Ten fish species, 30 individuals collected July 2007 (Total – 300 fish)

•Emphasis during Sept/October- fathead minnow and emerald shiner to look for Gyrodactylus hoffmaniGyrodactylus hoffmani

Total – 300 fish

• GRAND TOTAL FOR PARASITOLOGY - 900 FISH

• Fish examined for Internal and external parasites: all metazoan and protozoan parasites recorded.

• Eleven species of fish examined: northern pike, walleye whitebass, brook stickleback, fathead minnow, yellow perch, goldeye, emerald shiner, sauger, channel catfish, common shiner.

3232

PARASITES 2007PARASITES 200725 parasites detected in 2007; 8 more than in 2006. Total 25 parasites detected in 2007; 8 more than in 2006. Total parasites 2007-08 = 28 species.parasites 2007-08 = 28 species.

Gyrodactylus hoffmani Gyrodactylus hoffmani widespread in Red River basin in widespread in Red River basin in Canada on fathead minnow. Also in Devils Lake and Lake Canada on fathead minnow. Also in Devils Lake and Lake Traverse.Traverse.

Invasive Asian tapeworm widespread especially in Emerald Invasive Asian tapeworm widespread especially in Emerald Shiner but also found in 6 other species of fishShiner but also found in 6 other species of fish

Asian tapeworm found at international boundary. Not detected Asian tapeworm found at international boundary. Not detected in U.S. Potential for transfer of invasive species from Canada in U.S. Potential for transfer of invasive species from Canada to U.S.to U.S.

Blooding dwelling trematode, possibly Blooding dwelling trematode, possibly Sanguincola Sanguincola occidentalisoccidentalis prevalent in walleye (27 out of 60 infected). prevalent in walleye (27 out of 60 infected).

3333

2006-2007 PARASITOLOGY2006-2007 PARASITOLOGY

PARASITESPARASITES DEVILS LAKEDEVILS LAKE

20062006

624 fish624 fish



20072007

407 fish407 fish



2006 - 20072006 - 2007

Sheyenne and Sheyenne and Red RiversRed Rivers

Lake TraverseLake Traverse

RED RIVER RED RIVER BASINBASIN

2006 - 20072006 - 2007

CanadaCanada

Apiosoma sp.Apiosoma sp. ++ ++

AchtheresAchtheres sp sp ++

TrichodinaTrichodina sp sp ++ ++ ++ ++

CleidodiscusCleidodiscus sp. sp. ++

CamallanusCamallanus sp sp ++ ++

CorallobrothriumCorallobrothrium sp sp ++

Dactylogyrus sp.Dactylogyrus sp. ++ ++ ++

Diplostomum spathaceumDiplostomum spathaceum ++

Epistylus sp.Epistylus sp. ++ ++

ErgasilusErgasilus sp. sp. ++ ++

Gyrodactylus hoffmaniGyrodactylus hoffmani ++ ++ ++ ++

Ichthyobodo necatorIchthyobodo necator ++

Piscicola punctataPiscicola punctata ++

Paurorhynchus hiodontisPaurorhynchus hiodontis ++ ++

Pomphorhynchus bulbocolliPomphorhynchus bulbocolli ++ ++

3434

PARASITES-ContinuedPARASITES-Continued DEVILS LAKEDEVILS LAKE

20062006

624 fish624 fish



20072007

407 fish407 fish


RED RIVER RED RIVER BASIN USA-BASIN USA-2006 - 20072006 - 2007

Sheyenne and Sheyenne and Red RiversRed Rivers

Lake TraverseLake Traverse

RED RIVER RED RIVER BASINBASIN

2006 - 20072006 - 2007

CanadaCanada

AncyrocephalidaeAncyrocephalidae ++

Posthodiplostomum Posthodiplostomum sp.sp. ++ ++

Myzobdella lugubrisMyzobdella lugubris ++ ++

Neascus sp.Neascus sp. ++ ++

Onchocleidus chrysopsOnchocleidus chrysops ++ ++

Bothriocephalus cuspidatusBothriocephalus cuspidatus ++ ++ ++

Proteocephalus pinguisProteocephalus pinguis ++ ++ ++ ++

HexamitaHexamita sp. sp. ++

HenneguyaHenneguya sp sp ++

Ligula intestinalisLigula intestinalis ++ ++

Microcotyle spMicrocotyle sp ++

MyxobolusMyxobolus sp sp ++

Raphidascaris acusRaphidascaris acus ++ ++

SpinitectusSpinitectus sp. sp. ++

SpiroxysSpiroxys sp. sp. ++

Contracecum Contracecum sppspp ++ ++ ++ ++

3535

HISTOPATHOLOGY HISTOPATHOLOGY 2006-072006-07

Light microscopy examinationLight microscopy examination

Ten species: Emerald shiner, Yellow perch, Fathead minnow, Ten species: Emerald shiner, Yellow perch, Fathead minnow, Brook stickleback, White bass, Channel catfish, Goldeye, Brook stickleback, White bass, Channel catfish, Goldeye, Sauger, Walleye, Northern pike.Sauger, Walleye, Northern pike.

Sixty fish examined of each species in 2006; 30 fish examined Sixty fish examined of each species in 2006; 30 fish examined for each species in spring 2007 samplefor each species in spring 2007 sample

Total fish for histopathology 900.Total fish for histopathology 900.

Interim report submitted July 2007. Final report completed, and Interim report submitted July 2007. Final report completed, and under review at Environment Canada.under review at Environment Canada.

3636

myocardiumepicardium

penetratingwound

hemorrhage

W Figure 3 Myocardial penetrating wound- 1/60

3737

Dermal sarcoma - 2/60

sarcoma

epidermis W Figure 9

3838

HISTOPATHOLOGY HISTOPATHOLOGY 2006-072006-07Majority of agents found were metazoan and Majority of agents found were metazoan and protozoan parasites that caused local tissue protozoan parasites that caused local tissue reactionsreactions

No indication of clinical disease resulting from No indication of clinical disease resulting from bacterial or viral agents for any fish species in any bacterial or viral agents for any fish species in any location. Lesions associated with bacterial and viral location. Lesions associated with bacterial and viral agents were rareagents were rare

Lesions present in tissues would be expected of wild Lesions present in tissues would be expected of wild fishfish

Intracardial trematode Intracardial trematode Sanguincola occidentalisSanguincola occidentalis may may affect walleye healthaffect walleye health

3939

BACTERIOLOGY CanadaBACTERIOLOGY Canada

In 2006, 10 species and a total of 547 fish were In 2006, 10 species and a total of 547 fish were assessed for bacterial isolates.assessed for bacterial isolates.

In 2007, 10 species and a total of 508 fish were In 2007, 10 species and a total of 508 fish were assessed for bacterial isolatesassessed for bacterial isolates

In 2008 10 fish for a total of 583 fish were assessed In 2008 10 fish for a total of 583 fish were assessed for bacterial isolates. for bacterial isolates.

TOTAL FISH FOR BACTERIAL ASSESSMENT 1638TOTAL FISH FOR BACTERIAL ASSESSMENT 1638

4040

BACTERIOLOLGY 2006-2007BACTERIOLOLGY 2006-2007

GOOD NEWSGOOD NEWS

Renibacterium salmoninarum Renibacterium salmoninarum (bacterial kidney (bacterial kidney disease) not detected.disease) not detected.

Myxobolus cerebralisMyxobolus cerebralis (whirling disease) not detected (whirling disease) not detected in 2006 in lake whitefish in Lake Winnipeg.in 2006 in lake whitefish in Lake Winnipeg.

4141

BACTERIOLOGY CanadaBACTERIOLOGY Canada

BacteriumBacterium 20062006 20072007 20082008

Acinetobacter lwoffiiAcinetobacter lwoffii ++

Aeromonas hydrophilaAeromonas hydrophila ++ + + ++

Alcaligenes spp).Alcaligenes spp). ++

Enterobacter agglomeransEnterobacter agglomerans ++ ++

Edwardsiella tardaEdwardsiella tarda ++ ++

Flavobacterium sp.Flavobacterium sp. ++

Hafnia alveiHafnia alvei ++

Klebsiella oxytocaKlebsiella oxytoca ++

Plesiomonas shigelloides,Plesiomonas shigelloides, ++ ++ ++

Pseudomonas aeruginosaPseudomonas aeruginosa ++ ++

Pseudomonas fluorescensPseudomonas fluorescens ++ ++

Pseudomonas paucimobilia Pseudomonas paucimobilia ++

Pseudomonas maltophiliaPseudomonas maltophilia ++

4242

Bacteria Species Not Detected In Bacteria Species Not Detected In Previous YearsPrevious Years

0

1

2

3

4

5

6

2006 2007 2008

4343

BacteriumBacterium 2005-20062005-2006



20072007



20082008



Detected in Red Detected in Red River basinRiver basin

US andUS and

CanadaCanada

2006 - 20082006 - 2008

Acinetobacter lwoffiiAcinetobacter lwoffii ++ CanadaCanada

Aeromonas hydrophilaAeromonas hydrophila ++ + + ++ USA + CanadaUSA + Canada

Brevundimonas diminutaBrevundimonas diminuta ++

Corynebacterium renaleCorynebacterium renale ++

Hafnia alveiHafnia alvei ++ ++ ++ USA + CanadaUSA + Canada

Pseudomonas fluorescensPseudomonas fluorescens ++ USA + CanadaUSA + Canada

Pleisomonas shigelloidesPleisomonas shigelloides ++ CanadaCanada

Pseudomonas Pseudomonas spsp ++

Pseudomonas mendocinaPseudomonas mendocina ++ ++

Shewanella putrifaciensShewanella putrifaciens ++ ++

Streptococcus sobrinsStreptococcus sobrins ++

Yokenella regensburgiiYokenella regensburgii ++

BacteriologyDevils Lake versus Red River Basin

4444

VIROLOGYVIROLOGY

GOOD NEWSGOOD NEWSNine species of fish collected in fall 2006 – total of 547 fish.Nine species of fish collected in fall 2006 – total of 547 fish.

Ten species of fish collected in spring 2007 – total 508 fish.Ten species of fish collected in spring 2007 – total 508 fish.

Ten species of fish collected in spring 2008 - total 583 fish.Ten species of fish collected in spring 2008 - total 583 fish.

Total number of fish screened for targeted bacterial and viral Total number of fish screened for targeted bacterial and viral pathogens: 1638pathogens: 1638

No cytopathic effect observed in cell culture: no viral No cytopathic effect observed in cell culture: no viral agents detectedagents detected

4545

PARASITES AND PATHOGENSPARASITES AND PATHOGENSCANADA CANADA

Project managementProject management:: David Donald, Susan CosensDavid Donald, Susan Cosens

Fish collections: Fish collections: Douglas Watkinson, William Franzin, North-South Douglas Watkinson, William Franzin, North-South Consultants (Richard Remnant, Lee Murray, Darcy Pisiak, Terry Consultants (Richard Remnant, Lee Murray, Darcy Pisiak, Terry Neepin, Julie Brunel, Pat Nelson), Jim Gibson, Neepin, Julie Brunel, Pat Nelson), Jim Gibson,

Ken RowesKen Rowes

Parasitology:Parasitology: (University of Manitoba) – Terry Dick, Collin Gallagher (University of Manitoba) – Terry Dick, Collin Gallagher

Histopathology:Histopathology: (University of Guelph)– John Lumsden, Spencer Russel, (University of Guelph)– John Lumsden, Spencer Russel, Nikki LePage, Samantha AllenNikki LePage, Samantha Allen

Bacteriology/Virology:Bacteriology/Virology: (Fisheries and Oceans Canada)– Brian Souter, (Fisheries and Oceans Canada)– Brian Souter, Andy Dwilow, Sandra Aldous, Jason Allen, Ken RowesAndy Dwilow, Sandra Aldous, Jason Allen, Ken Rowes

4646

• Secure funding for completion of 2008 U.S. and Canada samples and data analyses

• Secure funding for 2009 for fall workshop (risk assessment)

• Secure funding for final report – U.S. and Canada

• Complete summary report with risk assessment using existing parasite and pathogen data (from 2009 workshop: Donald and Nelson - leads)

- undertake steps to secure peer review

• Complete draft, final report (contract to US and Canadian experts)

- undertake steps to secure peer review

• Complete final report

• Timeline for Completion: September 2010

Next Steps

devils lake, sheyenne river, red river and lake winnipeg

Documents

red river basin

red river delta

sheyenne river

lake winnipeg2

international red river

devils lake subbasin

lake traverse blc

multiple fish species