do it with electrons !. microscopy structure determines properties we have discussed crystal...
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![Page 1: Do it with electrons !. Microscopy Structure determines properties We have discussed crystal structure (x-ray diffraction) But consider now different](https://reader035.vdocuments.net/reader035/viewer/2022062516/56649d985503460f94a82db3/html5/thumbnails/1.jpg)
Do it with electrons !
Do it with electrons !
![Page 2: Do it with electrons !. Microscopy Structure determines properties We have discussed crystal structure (x-ray diffraction) But consider now different](https://reader035.vdocuments.net/reader035/viewer/2022062516/56649d985503460f94a82db3/html5/thumbnails/2.jpg)
MicroscopyMicroscopy
Structure determines propertiesStructure determines properties
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
Microstructure ( 微观结构 )- also affects propertiesMicrostructure ( 微观结构 )- also affects properties
Grey cast iron ( 灰色生铁 ) - rather brittle
Grey cast iron ( 灰色生铁 ) - rather brittle
Ductile iron - highly ductileDuctile iron - highly ductile
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MicroscopyMicroscopy
Structure determines propertiesStructure determines properties
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
Microstructure - also affects propertiesMicrostructure - also affects properties
Cemented WC ( 碳化钨 )cutting tool
Cemented WC ( 碳化钨 )cutting tool
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MicroscopyMicroscopy
Structure determines propertiesStructure determines properties
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
Microstructure - also affects propertiesMicrostructure - also affects properties
Ferroelectric domains in BaTiO3
Ferroelectric domains in BaTiO3
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MicroscopyMicroscopy
Structure determines propertiesStructure determines properties
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
We have discussed crystal structure (x-ray diffraction)
But consider now different level of structure
Microstructure - also can be 'art ( 美术 )'Microstructure - also can be 'art ( 美术 )'
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Electron microscopyElectron microscopy
SEM - scanning electron microscopy
tiny electron beam scanned across surface of specimen
backscattered ( 背散射 ) or
secondary electrons ( 二次电子 ) detected
signal output to synchronized display
SEM - scanning electron microscopy
tiny electron beam scanned across surface of specimen
backscattered ( 背散射 ) or
secondary electrons ( 二次电子 ) detected
signal output to synchronized display
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Electron microscopyElectron microscopy
SEM - scanning electron microscopy
Magnification range 15x to 200,000x
Resolution of 50 Å
Excellent depth of focus
Relatively easy sample prep
SEM - scanning electron microscopy
Magnification range 15x to 200,000x
Resolution of 50 Å
Excellent depth of focus
Relatively easy sample prep
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SEM - scanning electron microscopySEM - scanning electron microscopy
Electron gunElectron gun
Don't make x-rays - use electrons directly Don't make x-rays - use electrons directly
Wavelength:
NOT = hc/E (massless photons)
= h/(2melectronqVo)1/2
(non-relativistic)
= h/(2melectronqVo + q2Vo2/c2)1/2
(relativistic ( 相对论的 ))
Wavelength:
NOT = hc/E (massless photons)
= h/(2melectronqVo)1/2
(non-relativistic)
= h/(2melectronqVo + q2Vo2/c2)1/2
(relativistic ( 相对论的 ))
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SEM - scanning electron microscopySEM - scanning electron microscopy
= h / (2melectronqVo + q2Vo2/c2)1/2
= 1.22639 / (Vo + 0.97845 · 10-6Vo2)1/2
(nm) & Vo(volts)
= h / (2melectronqVo + q2Vo2/c2)1/2
= 1.22639 / (Vo + 0.97845 · 10-6Vo2)1/2
(nm) & Vo(volts)
10 kV ——> 0.12 Å
100 kV ——> 0.037 Å
10 kV ——> 0.12 Å
100 kV ——> 0.037 Å
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SEM - scanning electron microscopySEM - scanning electron microscopy
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SEM - scanning electron microscopySEM - scanning electron microscopy
QuickTime™ and a decompressor
are needed to see this picture.
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are needed to see this picture.
Wehnelt
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SEM - scanning electron microscopySEM - scanning electron microscopy
x-rays
composition
backscattered e's
microstructure
secondary e's
microstructure
elastically scattered e's
crystallographic structure
inelastically scattered e's
composition
transmitted e's
microstructure
SIGNALS IN ELECTRON MICROSCOPY
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SEM - scanning electron microscopySEM - scanning electron microscopy
Electron gunElectron gun
Electron emitterElectron emitter
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SEM - scanning electron microscopySEM - scanning electron microscopy
Effects of increasing voltage in electron gun:
Resolution increased ( decreased)
Penetration increases
Specimen charging increases (insulators)
Specimen damage increases
Image contrast decreases
Effects of increasing voltage in electron gun:
Resolution increased ( decreased)
Penetration increases
Specimen charging increases (insulators)
Specimen damage increases
Image contrast decreases
= h/(2melectronqVo + q2Vo2/c2))1/2
= h/(2melectronqVo + q2Vo2/c2))1/2
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SEM - scanning electron microscopySEM - scanning electron microscopy
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
maybe
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SEM - scanning electron microscopySEM - scanning electron microscopy
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
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SEM - scanning electron microscopySEM - scanning electron microscopy
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
cool tip ——> smaller E in beam improved coherence
many electrons from small tip ——> finer probe size, higher current densities (100X >) problems - high vacuum, more $$$, fussy
Field emission electron source:
High electric field at very sharp tip causes electrons to "tunnel"
cool tip ——> smaller E in beam improved coherence
many electrons from small tip ——> finer probe size, higher current densities (100X >) problems - high vacuum, more $$$, fussy
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SEM - scanning electron microscopySEM - scanning electron microscopy
Lenses
electrons focused by Lorentz force from electromagnetic field
F = qv x B
effectively same as optical lenses
Lenses
electrons focused by Lorentz force from electromagnetic field
F = qv x B
effectively same as optical lenses
Lenses are ring-shaped
coils generate magnetic field electrons pass thru hollow center
lens focal length is continuously variable
apertures control, limit beam
Lenses are ring-shaped
coils generate magnetic field electrons pass thru hollow center
lens focal length is continuously variable
apertures control, limit beam
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SEM - scanning electron microscopySEM - scanning electron microscopy
Specimen Specimen
Conducting - little or no preparation attach to mounting stub
for insertion into instrument
may need to provide conductive path
with Ag paint
Conducting - little or no preparation attach to mounting stub
for insertion into instrument
may need to provide conductive path
with Ag paint
Non-conducting - usually coat with conductive very thin layer (Au, C,
Cr)
Non-conducting - usually coat with conductive very thin layer (Au, C,
Cr)
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SEM - scanning electron microscopySEM - scanning electron microscopy
Specimen Specimen
Can examine fracture surfaces electronic devices fibers coatings particles etc.
Can examine fracture surfaces electronic devices fibers coatings particles etc.
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SEM - scanning electron microscopySEM - scanning electron microscopy
Specimen Specimen
Can be tilted, translated
Specimen size limited by size of sample chamber
Can be tilted, translated
Specimen size limited by size of sample chamber
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SEM - scanning electron microscopySEM - scanning electron microscopy
Specimen Specimen
What comes from specimen?
Backscattered electrons
Secondary electrons
Fluorescent X-rays
What comes from specimen?
Backscattered electrons
Secondary electrons
Fluorescent X-rays
high energycompositional contrasthigh energycompositional contrast
low energytopographic contrastlow energytopographic contrastcomposition - EDScomposition - EDS
Brightness of regions in image increases as atomic number increases
Brightness of regions in image increases as atomic number increases(less penetration gives more backscattered electrons)(less penetration gives more backscattered electrons)
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SEM - scanning electron microscopySEM - scanning electron microscopy
Electron energy distribution Electron energy distribution
BSEsSEs
E/Eo
1
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SEM - scanning electron microscopySEM - scanning electron microscopy
Backscattered electron detector - solid state detector Backscattered electron detector - solid state detector
electron energy up to 30-50 keV
annular around incident beam
repel secondary electrons with — biased mesh
electron energy up to 30-50 keV
annular around incident beam
repel secondary electrons with — biased mesh
images are more sensitive to chemical composition (electron yield depends on atomic number)
line of sight necessary
images are more sensitive to chemical composition (electron yield depends on atomic number)
line of sight necessary
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SEM - scanning electron microscopySEM - scanning electron microscopy
Secondary electron detector - scintillation detector Secondary electron detector - scintillation detector
+ bias mesh needed in front of detector to attract low energy electrons
line of sight unnecessary
+ bias mesh needed in front of detector to attract low energy electrons
line of sight unnecessary
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SEM - scanning electron microscopySEM - scanning electron microscopy
Secondary electron detector - scintillation detector Secondary electron detector - scintillation detector
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SEM - scanning electron microscopySEM - scanning electron microscopy
Choose correct detector- topography exampleChoose correct detector- topography example
Fracture surface in ironFracture surface in iron
backscattered electrons secondary electronsbackscattered electrons secondary electrons
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SEM - scanning electron microscopySEM - scanning electron microscopy
Composition - what elements present at a particular spot in specimen?Composition - what elements present at a particular spot in specimen?
Use solid state detector
Do energy scan for fluorescent X-rays
Use solid state detector
Do energy scan for fluorescent X-rays
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SEM - scanning electron microscopySEM - scanning electron microscopy
Composition mapping - x-ray fluorescenceComposition mapping - x-ray fluorescence
Use solid state detector set for X-ray energy for a particular element in specimenUse solid state detector set for X-ray energy for a particular element in specimen
image X-ray mapimage X-ray map
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SEM - scanning electron microscopySEM - scanning electron microscopy
Interaction volume Interaction volume
Backscattered electrons come from whole volume (high energy)
Backscattered electrons come from whole volume (high energy)
Secondary electrons come from neck only (low energy)
Secondary electrons come from neck only (low energy)
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SEM - scanning electron microscopySEM - scanning electron microscopy
Contrast Contrast
Comes from any kind of interaction with electron beam topography composition elements phases grain (crystal) orientation
charging affects contrast
Comes from any kind of interaction with electron beam topography composition elements phases grain (crystal) orientation
charging affects contrast
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SEM - scanning electron microscopySEM - scanning electron microscopy
Magnification Magnification
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SEM - scanning electron microscopySEM - scanning electron microscopy
Resolution Resolution
Determined by probe size
Determined by probe size
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SEM - scanning electron microscopySEM - scanning electron microscopy
Resolution Resolution
Determined by probe size
Determined by probe size
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SEM - scanning electron microscopySEM - scanning electron microscopy
Depth of field Depth of field
d = depth of field = required spatial resolution= convergence angle
d
region of image in focus
tan =0.50.5d
=d
For small angles, tan =
d =
Can control depth of field (d) with convergence angle ()
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SEM - scanning electron microscopySEM - scanning electron microscopy
Depth of field Depth of field
WD
Rap
tanα =Rap
WD