Coordinated by Claudie PavisINRA (National Institute for Agronomic Research, France)
SafePGR: Towards Safer Plant Genetic Resources through improved viral diagnostics
NET-BIOME FINAL CONFERENCE15 February 2012, Paris
1- INRA Guadeloupe (UR ASTRO)2- Cirad Guadeloupe (TGU AGAP)3- Cirad Montpellier (UMR BGPI)4- Cirad La Réunion (UMR PVBMT) 5- INRA Bordeaux (UMR BFP)6- Biotechnology Centre Azores (University)7- ISOPlexis Gene Bank Madeira (University)
1) Partner’s consortium, budget and agenda
Project’s duration: 3 years - March 2012 to February 2015
91 k€ 53 k€180 k€ 33 k€100 k€ 51 k€ 45 k€
Total budget: 1 450 k€ - Requested contribution: 553 k€
1- Inra Guadeloupe (UR ASTRO)2- Cirad Guadeloupe (TGU AGAP)3- Cirad Montpellier (UMR BGPI)4- Cirad La Réunion (UMR PVBMT) 5- Inra Bordeaux (UMR BFP)6- Biotechnology Centre Azores (University)7- ISOPlexis Gene Bank Madeira (University)
1) Partner’s consortium, budget and agenda
2) The five goals of the SafePGR project Develop reliable and portable tools for detecting viruses conserved in tropical and subtropical germplasm collections
Assay the viral biodiversity present within crops that are critical to the agriculture of Guadeloupe, Réunion, Azores and Madeira
Develop regional expertise in the field of viral diagnosis
Facilitate sanitation procedures within biological ressource centres (BRCs)
Secure the dissemination of virus-free germplasm to extension services (within and between European overseas territories)
Improve knowledge on the diversity of viruses infecting crops targeted
by the project : banana, garlic, sugarcane, sweet potato, vanilla and yam
3) Objectives
Develop optimized and portable diagnostic methods for the routine detection of viruses in these 6 crops
Develop bioinformatics tools available for all the partners of the project
What is the molecular diversity of reported (« already-known ») viruses that are present in the main crops conserved in BRCs in Azores, Guadeloupe, Madeira and Réunion?
Conversely, are conventional diagnostic methods best suited to cover the molecular diversity of known virus species infecting crops targeted by the project?
Are metagenomics approaches efficient and portable for improved viral diagnostic?
What are the global and local population structures of the viruses infecting tropical conserved crops? Could we decipher routes of transmissions of these viruses?
3) Research questions
What is the virome (the genomes of all the – known and unknown - viruses that inhabit a particular organism) of plants conserved in BRCs?
Broad inventory of inter and intraspecific viral diversity in crops targeted by the project
Effectiveness of quarantine and sanitation procedures
Safer germplasm movement from BRCs by preventing the introduction and spread of viral diseases
4) Expected results
Capacity-building in modern diagnostic tools for virology labs of Guadeloupe, Azores, Madeira and La Réunion
Enhancement of the management of germplasm collections through the ability of BRCs to carry out:
reliable viral diagnostic safer germplasm movement
4) Perspectives for development
Increased diversity within cropping systems:
Virus-free germplasm is necessary to breeding programmes, which aim at developping improved varieties adapted to changing biotic, climatic and socio-economic contexts
Thank you for your
attention!
A website is already available!
http://internet.antilles.inra.fr/astro/safepgr/