![Page 1: Device to apply a coating on tissue samples for MALDI imaging Holly Liske Laura Piechura Kellen Sheedy Jenna Spaeth Amy Harms, PhD UW Biotechnology Center](https://reader035.vdocuments.net/reader035/viewer/2022062515/56649cc05503460f949874dc/html5/thumbnails/1.jpg)
Device to apply a coating on tissue
samples for MALDI imaging
Holly LiskeLaura PiechuraKellen SheedyJenna Spaeth
Amy Harms, PhDUW Biotechnology Center
Brenda Ogle, PhDDepartment of Biomedical Engineering
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Presentation Overview
Background of MALDI imaging Project motivation Problem statement Design criteria Design matrices Final design Budget evaluation Future work
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MALDI-MSI
www.mc.vanderbilt.edu
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Data Acquisition from MALDI-MSI
Alanine, neuropeptide in crab brain m/z = 1474.6
Valine, m/z = 1502.7
Alanine
Valine
Greg Barrett-Wilt, PhD., UW Biotechnology Center
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Benefits of MALDI-MSI Analysis of entire
sample in one reading
Previous knowledge of molecular composition is not necessary
Allows for investigation of disease formation, progression, and treatment
www.maldi-msi.org
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Matrix Application
Matrix application is vital for quality image resolution
Must contact sample as fine, liquid mist
Current procedure involves manual application with airbrush
100µm raster step
Greg Barrett-Wilt, PhD., UW Biotechnology Center
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Problem Statement
Sample preparation methods MALDI-MSI are difficult to control
Requires accuracy and precision
A device to apply a fine, uniform coating of light-absorbing compounds
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Project Motivation Objective 1:
Simplify matrix application, facilitate MALDI
Objective 2: Centralize a reliable
tool at UW Biotechnology Center
Implications for campus-wide, even global, research
www.buswire.ocr.wisc.edu
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Design Criteria
Spray an even coating of matrix over an 81cm x 123 cm tissue sample
Adjustable spray aperture, air pressure and positioning of the plate and sprayer
Enclosed in a casing and operable within a fume hood
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Design Matrix Adjustment
Several variables controlled by many independent components
Sprayer Method of application Enclosure Orientation
Created a component matrix to decide final design
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Component Matrix
VerticalHorizontalOrientation
Detached covering
Integrated covering
Fume hoodEnclosure
Stationary sprayer,Movable
plate
Movable sprayer,
Stationary plate
Method of Application
Produce irrigation system
Pneumatic sprayer
Nebulizer
Nozzle-valve with pressure vessel
Automatic Spray Gun Sprayer
Ideas Feature
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Criteria for ranking
Reliability Adjustability User interaction Cost Maintenance Ease of manufacture
Ranking System (1-5), highest = best
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Mega MatrixSpraying Component
2.354.104.303.452.251.00Total
214310.05Ease of
Manufacture
243310.10Maintenance
315340.15Cost
354450.20User
Interaction
255410.25Adjustability
254310.25Reliability
Produce irrigation system
Pneumatic sprayer
Automatic Spray Gun
Nozzle-valve and
pressure vessel
NebulizerRankCriteria
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Mega MatrixMethod of Application
3.252.851.00Total
130.05
Ease ofManufacture
330.10Maintenance
420.15Cost
430.20
User Interaction
330.25Adjustability
330.25
Reliability
Movable Plate Stationary Sprayer
Stationary Plate Movable Sprayer
RankCriteria
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Mega MatrixEnclosure
2.452.91.551.00Total
4350.05Ease of
Manufacture
5410.10
Maintenance
3250.15
Cost
4410.20User
Interaction
2510.25
Adjustability
---0.25Reliability
Removable CoverIntegrated CoverFume HoodRankCriteria
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Mega MatrixOrientation
1.25.51.00Total
--0.05Ease of
Manufacture
--0.10Maintenance
--0.15Cost
--0.20User
Interaction
--0.25
Adjustability
520.25
Reliability
Vertical SprayHorizontal
SprayRankCriteria
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Final Design Model
1. Automatic Spray Gun
2. Conveyor
3. DC Timing Motor
4. Integrated Polyethylene Box
14
3
2
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Budget Evaluation
$300.00 budget limit Breakdown
Air Spray Gun: $150.00 Motor/Conveyor: $45.00 Enclosure material: $45.00 Other small parts: $40.00
Total Estimate: $280.00 (under budget)
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Future Work
Final Design modifications Order materials Build prototype Test in client’s lab
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References Ashcroft, Alison. University of Leeds Astbury Centre for Structural
Molecular Biology. “An Introduction to Mass Spectrometry.” <http://www.astbury.leeds.ac.uk/>
Barrett-Wilt, Greg. University of Wisconsin Biotechnology Center. Personal interviews.
Caprioli Research Laboratory Center. Vanderbilt Medical Center.
<http://www.mcvanderbilt.edu> Khatib-Shahidi, Sheerin. “Direct Molecular Analysis of Whole-Body
Animal Tissue Section by Imaging MALDI Mass Spectrometry.” Analytical Chemistry. 2006, 78(18) 6448-6456.
Sugiura, Yuki. “Two-Step Matrix Application Technique to Improve Ionization Efficiency for MALDI-MSI.” Analytical Chemistry. 2006, 78(24) 8227-8235.
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Any Questions?