European Journal of Immunology
Supporting Information
for
Eur. J. Immunol. 10.1002/eji.200636270
Development of nephritis but not sialadenitis in autoimmune-prone BAFF
transgenic mice lacking marginal zone B cells
Carrie A. Fletcher, Andrew P. R. Sutherland, Joanna R. Groom, Marcel L. Batten,
Lai Guan Ng, Jennifer Gommerman and Fabienne Mackay
© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu
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1
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Weight (mg)
IgA
Tite
r (lo
g ba
se 2
)
0
1
2
3
4
5
6
7
8
Weight (mg)
IgM
Tite
r (lo
g ba
se 2
)
Day 0
WT
Day 0
LTβ∆
-BTg
Day 0
BAFF Tg
Day 0
LTβ-/
-
Day 7
WT
Day 7
LTβ∆
-BTg
Day 7
BAFF Tg
Day 7
LTβ-/
-
Day 0
WT
Day 0
LTβ∆
-BTg
Day 0
BAFF Tg
Day 0
LTβ-/
-
Day 7
WT
Day 7
LTβ∆
-BTg
Day 7
BAFF Tg
Day 7
LTβ-/
-
Supplementary Figure 1: T-independent immune response to NP Ficoll. NP-specific IgA (left panel) and IgM (right panel) Ab responses from indicated mice at day 0 and 7 days after immunization with the T-independent Ag NP-Ficoll, symbols represent individual mice and lines indicate mean values.
Supplementary Figure 1. Fletcher et al 2006
B-1bB-1aFollicular
Salivary Gland
Cel
l N
umbe
rs (x
104 )
WT
LTβ∆
-BTg
BAFF TgLT
β-/-
WT
LTβ∆
-BTg
BAFF TgLT
β-/-
WT
LTβ∆
-BTg
BAFF TgLT
β-/-
0
10
20
30
40
50
0
10
20
30
40
50
0
10
20
30
40
50
Supplementary Figure 2. Fletcher et al 2006
Supplementary Figure 2: Enumeration of B lymphocyte subsets infiltrating the salivary gland of 12-14-mo-old WT (o), LTβ—/— (◊), BAFF Tg (Ο) and LTβ∆-BTg (∆) mice, Follicular B cells (left panel), B-1a B cells (Middle panel) and B-1b B cells (right panel) revealed increased absolute numbers of follicular B cells, but not B-1 B cells infiltrating the salivary gland of LTβ∆-BTg. Symbols repre-sent individual mice and lines indicate mean values