European Journal of Immunology

Supporting Information

for

Eur. J. Immunol. 10.1002/eji.200636270

Development of nephritis but not sialadenitis in autoimmune-prone BAFF

transgenic mice lacking marginal zone B cells

Carrie A. Fletcher, Andrew P. R. Sutherland, Joanna R. Groom, Marcel L. Batten,

Lai Guan Ng, Jennifer Gommerman and Fabienne Mackay

© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu

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Weight (mg)

IgA

Tite

r (lo

g ba

se 2

)

0

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Weight (mg)

IgM

Tite

r (lo

g ba

se 2

)

Day 0

WT

Day 0

LTβ∆

-BTg

Day 0

BAFF Tg

Day 0

LTβ-/

-

Day 7

WT

Day 7

LTβ∆

-BTg

Day 7

BAFF Tg

Day 7

LTβ-/

-

Day 0

WT

Day 0

LTβ∆

-BTg

Day 0

BAFF Tg

Day 0

LTβ-/

-

Day 7

WT

Day 7

LTβ∆

-BTg

Day 7

BAFF Tg

Day 7

LTβ-/

-

Supplementary Figure 1: T-independent immune response to NP Ficoll. NP-specific IgA (left panel) and IgM (right panel) Ab responses from indicated mice at day 0 and 7 days after immunization with the T-independent Ag NP-Ficoll, symbols represent individual mice and lines indicate mean values.

Supplementary Figure 1. Fletcher et al 2006

B-1bB-1aFollicular

Salivary Gland

Cel

l N

umbe

rs (x

104 )

WT

LTβ∆

-BTg

BAFF TgLT

β-/-

WT

LTβ∆

-BTg

BAFF TgLT

β-/-

WT

LTβ∆

-BTg

BAFF TgLT

β-/-

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Supplementary Figure 2. Fletcher et al 2006

Supplementary Figure 2: Enumeration of B lymphocyte subsets infiltrating the salivary gland of 12-14-mo-old WT (o), LTβ—/— (◊), BAFF Tg (Ο) and LTβ∆-BTg (∆) mice, Follicular B cells (left panel), B-1a B cells (Middle panel) and B-1b B cells (right panel) revealed increased absolute numbers of follicular B cells, but not B-1 B cells infiltrating the salivary gland of LTβ∆-BTg. Symbols repre-sent individual mice and lines indicate mean values